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https://www.readbyqxmd.com/read/28627450/impact-of-fixation-artifacts-and-threshold-selection-on-high-resolution-melting-analysis-for-kras-mutation-screening
#1
Wendy Pérez-Báez, Ethel A Garcia-Latorre, Hector Aquiles Maldonado-Martinez, Iris Coronado-Martinez, Leonardo Flores-Garcia, Lucía Taja-Chayeb
INTRODUCTION: Treatment in the metastatic colorectal cancer has expanded with monoclonal antibodies targeting Epidermal Growth Factor Receptor, but is restricted to patients with a wild-type (WT) KRAS mutational status. The most sensitive assays for KRAS mutation detection in formalin-fixed paraffin embedded (FFPE) tissues are based on Real-time PCR. Among them, high resolution melting analysis (HRMA), is a simple, fast, highly sensitive, specific and cost-effective method, proposed as adjunct for KRAS mutation detection...
June 13, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28536543/adoption-of-task-specific-sets-of-visual-attention
#2
Mike Wendt, Svantje T Kähler, Aquiles Luna-Rodriguez, Thomas Jacobsen
Evidence from behavioral and physiological studies suggests attentional weighting of stimulus information from different sources, according to task demands. We investigated the adoption of task-specific attentional sets by administering a flanker task, which required responding to a centrally presented letter while ignoring two adjacent letters, and a same-different judgment task, which required a homogenous/heterogeneous classification concerning the complete three-letter string. To assess the distribution of attentional weights across the letter locations we intermixed trials of a visual search task, in which a target stimulus occurred randomly in any of these locations...
2017: Frontiers in Psychology
https://www.readbyqxmd.com/read/28484204/hydrogen-sulfide-improves-cardiomyocyte-function-in-a-cardiac-arrest-model
#3
Nahuel Aquiles Garcia, Javier Moncayo-Arlandi, Alejandro Vazquez, Patricia Genovés, Conrado J Calvo, José Millet, Nuria Martí, Carmen Aguado, Erwin Knecht, Iñigo Valiente-Alandi, José A Montero, Antonio Díez-Juan, Pilar Sepúlveda
BACKGROUND Cardioplegic arrest is a common procedure for many types of cardiac surgery, and different formulations have been proposed to enhance its cardio-protective effect. Hydrogen sulfide is an important signaling molecule that has cardio-protective properties. We therefore studied the cardio-protective effect of hydrogen sulfide in cardiac cell culture and its potential therapeutic use in combination with cardioplegia formulations. MATERIAL AND METHODS We added hydrogen sulfide donor GYY4137 to HL-1 cells to study its protective effect in nutrient starved conditions...
May 9, 2017: Annals of Transplantation: Quarterly of the Polish Transplantation Society
https://www.readbyqxmd.com/read/28456306/characterization-and-establishment-of-a-reference-deltamethrin-and-cypermethrin-resistant-tick-line-ivri-iv-of-rhipicephalus-boophilus-microplus
#4
Srikant Ghosh, Snehil Gupta, K G Ajith Kumar, Anil Kumar Sharma, Sachin Kumar, Gaurav Nagar, Rinesh Kumar, Souvik Paul, Ashutosh Fular, Gajanan Chigure, Abhijit Nandi, H V Manjunathachar, Aquil Mohammad, M R Verma, B C Saravanan, Debdatta Ray
The problem of ticks and tick borne diseases is a global threat and growing reports of resistance to commonly used insecticides further aggravated the condition and demands for country specific resistance monitoring tools and possible solutions of the problem. Establishment of standard reference is prerequisite for development of monitoring tools. For studying possible role of different mechanisms involved in development of resistance in Rhipicephalus (Boophilus) microplus population and to develop newer drug to manage the problem of resistance, a deltamethrin exposed and selected tick colony, referred to as IVRI-IV, was characterized using reference susceptible IVRI-I tick line as control...
May 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28368156/-strategic-control-over-extent-and-timing-of-distractor-based-response-activation-correction-to-jost-et-al-2017
#5
(no author information available yet)
Reports an error in "Strategic control over extent and timing of distractor-based response activation" by Kerstin Jost, Mike Wendt, Aquiles Luna-Rodriguez, Andreas Löw and Thomas Jacobsen (Journal of Experimental Psychology: Learning, Memory, and Cognition, 2017[Feb], Vol 43[2], 326-333). In the article, the images to Figures 2 and 3 were switched during production. The online version of this article has been corrected. (The following abstract of the original article appeared in record 2016-48458-001.) In choice reaction time (RT) tasks, performance is often influenced by the presence of nominally irrelevant stimuli, referred to as distractors...
April 2017: Journal of Experimental Psychology. Learning, Memory, and Cognition
https://www.readbyqxmd.com/read/28356978/reproducibility-of-the-egfr-immunohistochemistry-scores-for-tumor-samples-from-patients-with-advanced-non-small-cell-lung-cancer
#6
Alejandro Avilés-Salas, Saé Muñiz-Hernández, Héctor Aquiles Maldonado-Martínez, José G Chanona-Vilchis, Laura-Alejandra Ramírez-Tirado, Norma HernáNdez-Pedro, Rita Dorantes-Heredia, José Manuel RuíZ-Morales, Daniel Motola-Kuba, Oscar Arrieta
Epidermal growth factor receptor (EGFR) is overexpressed in >60% of non-small cell lung cancer (NSCLC) cases. In combination with radiotherapy or chemotherapy, first-line treatments with antibodies against EGFR, including cetuximab and necitumumab, have demonstrated benefits by increasing overall survival (OS), particularly in patients who overexpress EGFR. The present study evaluated the interobserver agreement among three senior pathologists, who were blinded to the clinical outcomes and assessed tumor samples from 85 patients with NSCLC using the H-score method...
February 2017: Oncology Letters
https://www.readbyqxmd.com/read/28190980/effect-of-low-dose-short-term-creatine-supplementation-on-muscle-power-output-in-elite-youth-soccer-players
#7
Aquiles Yáñez-Silva, Cosme F Buzzachera, Ivan Da C Piçarro, Renata S B Januario, Luis H B Ferreira, Steven R McAnulty, Alan C Utter, Tacito P Souza-Junior
BACKGROUND: To determine the effects of a low dose, short-term Creatine monohydrate (Cr) supplementation (0.03 g.kg.d(-1) during 14 d) on muscle power output in elite youth soccer players. METHODS: Using a two-group matched, double blind, placebo-controlled design, nineteen male soccer players (mean age = 17.0 ± 0.5 years) were randomly assigned to either Cr (N = 9) or placebo (N = 10) group. Before and after supplementation, participants performed a 30s Wingate Anaerobic Test (WAnT) to assess peak power output (PPO), mean power output (MPO), fatigue index (FI), and total work...
2017: Journal of the International Society of Sports Nutrition
https://www.readbyqxmd.com/read/28018922/influence-of-the-expression-of-inflammatory-markers-on-kidney-after-fetal-programming-in-an-experimental-model-of-renal-failure
#8
Carlos Donizete Pereira Júnior, Camila Souza de Oliveira Guimarães, Aline Cristina Souza da Silva, Aldo Rogelis Aquiles Rodrigues, Maria Aparecida da Glória, Vicente de Paula Antunes Teixeira, Niels Olsen Saraiva Câmara, Lenaldo Branco Rocha, Marlene Antônia Dos Reis, Juliana Reis Machado, Laura Penna Rocha, Fernanda Rodrigues Helmo, Rosana Rosa Miranda Corrêa
Objective. To evaluate the expression of inflammatory markers in experimental renal failure after fetal programming. Methods. The offspring aged two and five months were divided into four groups: CC (control dams, control offspring); DC (diabetic dams, control offspring); CFA (control dams, folic acid offspring, 250 mg/Kg); and DFA (diabetic dams, folic acid offspring). Gene expression of inflammatory markers MCP-1, IL-1, NOS3, TGF-β, TNF-α, and VEGF was evaluated by RT-PCR. Results. MCP-1 was increased in the CFA and DFA groups at two and five months of age, as well as in DC5 when compared to CC5...
2016: Journal of Immunology Research
https://www.readbyqxmd.com/read/27973071/epidemiological-profiling-of-chronic-headache-patients-at-a-public-tertiary-care-setting-in-india
#9
A K Najmi, M U Hoda, S Hussain, A Habib, P Kapur, A Anwar, M Akhtar, M Aquil
No abstract text is available yet for this article.
November 2016: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/27926850/background-suppression-in-imaging-gold-nanorods-through-detection-of-anti-stokes-emission
#10
Aquiles Carattino, Veer I P Keizer, Marcel J M Schaaf, Michel Orrit
Metallic nanoparticles have opened the possibility of imaging, tracking, and manipulating biological samples without time limitations. Their low photoluminescence quantum yield, however, makes them hard to detect under high background conditions. In this study we show that it is possible to image gold nanorods by detecting their anti-Stokes emission under resonant excitation. We show that even in the membrane of a cell containing the fluorescent dye Atto 647N, the signal/background of the anti-Stokes emission can be >10, while it is impossible to image the particles with the Stokes emission...
December 6, 2016: Biophysical Journal
https://www.readbyqxmd.com/read/27832230/inflammatory-and-immunological-profiles-in-patients-with-copd-relationship-with-fev-1-reversibility
#11
Cleriston Farias Queiroz, Antonio Carlos Moreira Lemos, Maria de Lourdes Santana Bastos, Margarida Célia Lima Costa Neves, Aquiles Assunção Camelier, Natália Barbosa Carvalho, Edgar Marcelino de Carvalho
Objective: To determine whether COPD severity correlates with sputum cell counts, atopy, and asthma. Methods: This was a cross-sectional study involving 37 patients with COPD and 22 healthy subjects with normal lung function (controls). Sputum cell counts were determined by microscopy after centrifugation of samples. Skin prick tests were performed, and serum cytokines were determined by ELISA. Results: Patients were stratified by bronchodilator response: a non-reversible airflow limitation (nonRAL) group comprised 24 patients showing no significant post-bronchodilator change in FEV1; and a partially reversible airflow limitation (partialRAL) group comprised 13 patients showing FEV1 reversibility (post-bronchodilator FEV1 increase ≥ 12%)...
July 2016: Jornal Brasileiro de Pneumologia: Publicaça̋o Oficial da Sociedade Brasileira de Pneumologia e Tisilogia
https://www.readbyqxmd.com/read/27816203/-relationship-between-balance-tests-and-mobility-scale-fear-of-falling-and-the-number-of-falls-in-the-elderly-in-a-rehabilitation-department
#12
Haviley Oliveira Martins, Karoline Mayara de Aquiles Bernardo, Maristela Santini Martins, Fábio Marcon Alfieri
No abstract text is available yet for this article.
November 2, 2016: Revista Española de Geriatría y Gerontología
https://www.readbyqxmd.com/read/27741241/noninvasive-diagnosis-of-visceral-leishmaniasis-development-and-evaluation-of-two-urine-based-immunoassays-for-detection-of-leishmania-donovani-infection-in-india
#13
Sarfaraz Ahmad Ejazi, Pradyot Bhattacharya, Md Asjad Karim Bakhteyar, Aquil Ahmad Mumtaz, Krishna Pandey, Vidya Nand Ravi Das, Pradeep Das, Mehebubar Rahaman, Rama Prosad Goswami, Nahid Ali
BACKGROUND: Visceral Leishmaniasis (VL), a severe parasitic disease, could be fatal if diagnosis and treatment is delayed. Post kala-azar dermal leishmaniasis (PKDL), a skin related outcome, is a potential reservoir for the spread of VL. Diagnostic tests available for VL such as tissue aspiration are invasive and painful although they are capable of evaluating the treatment response. Serological tests although less invasive than tissue aspiration are incompetent to assess cure. Parasitological examination of slit-skin smear along with the clinical symptoms is routinely used for diagnosis of PKDL...
October 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27732019/strategic-control-over-extent-and-timing-of-distractor-based-response-activation
#14
Kerstin Jost, Mike Wendt, Aquiles Luna-Rodriguez, Andreas Löw, Thomas Jacobsen
In choice reaction time (RT) tasks, performance is often influenced by the presence of nominally irrelevant stimuli, referred to as distractors. Recent research provided evidence that distractor processing can be adjusted to the utility of the distractors: Distractors predictive of the upcoming target/response were more attended to and also elicited stronger motor responses. In an event-related potential (ERP) study, we investigated whether not only the extent of distractor processing (as suggested by these previous results), but also the timing of distractor-based response activation is subject to strategic control...
October 10, 2016: Journal of Experimental Psychology. Learning, Memory, and Cognition
https://www.readbyqxmd.com/read/27692692/recommendations-on-the-use-of-deep-neuromuscular-blockade-by-anaesthesiologists-and-surgeons-aquiles-anestesia-quir%C3%A3%C2%BArgica-para-lograr-eficiencia-y-seguridad-consensus
#15
C L Errando-Oyonarte, C Moreno-Sanz, P Vila-Caral, J C Ruiz de Adana-Belbel, E Vázquez-Alonso, J M Ramírez-Rodríguez, G Veiga-Ruiz, E Guasch-Arévalo, J I Lora-Tamayo D'Ocón
INTRODUCTION: Neuromuscular blockade enables airway management, ventilation and surgical procedures. However there is no national consensus on its routine clinical use. The objective was to establish the degree of agreement among anaesthesiologists and general surgeons on the clinical use of neuromuscular blockade in order to make recommendations to improve its use during surgical procedures. METHODS: Multidisciplinary consensus study in Spain. Anaesthesiologists experts in neuromuscular blockade management (n=65) and general surgeons (n=36) were included...
February 2017: Revista Española de Anestesiología y Reanimación
https://www.readbyqxmd.com/read/27412010/molecular-disturbance-underlies-to-arrhythmogenic-cardiomyopathy-induced-by-transgene-content-age-and-exercise-in-a-truncated-pkp2-mouse-model
#16
Javier Moncayo-Arlandi, Eduard Guasch, Maria Sanz, Marta Casado, Nahuel Aquiles García, Lluis Mont, Marta Sitges, Ralph Knöll, Byambajav Buyandelger, Campuzano Oscar, Antonio Diez-Juan, Ramon Brugada
Arrhythmogenic cardiomyopathy (ACM) is a disorder characterized by a progressive ventricular myocardial replacement by fat and fibrosis which lead to ventricular arrhythmias and sudden cardiac death. Mutations in the desmosomal gene Plakophilin-2 (PKP2) accounts for >40% of all known mutations, generally causing a truncated protein. In a PKP2 truncated mouse model, we hypothesize that content of transgene, endurance training and aging will be determinant in disease progression. In addition, we investigated the molecular defects associated with the phenotype in this model...
July 12, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/27383933/viability-of-gait-speed-test-in-hospitalized-elderly-patients
#17
Bruno Prata Martinez, Anne Karine Menezes Santos Batista, Isis Resende Ramos, Júlio Cesar Dantas, Isabela Barboza Gomes, Luiz Alberto Forgiarini, Fernanda Rosa Warken Camelier, Aquiles Assunção Camelier
OBJECTIVE: The gait speed test (GST) is a physical test that can predict falls and aid in the diagnosis of sarcopenia in the elderly. However, to our knowledge, there have been no studies evaluating its reproducibility in hospitalized elderly patients. The objective of this study was to evaluate the safety and reproducibility of the six-meter GST (6GST) in hospitalized elderly patients. METHODS: This repeated measures study involved hospitalized elderly patients (≥ 60 years of age) who underwent the 6GST by the fifth day of hospitalization, were able to walk without assistance, and presented no signs of dyspnea or pain that would prevent them from performing the test...
May 2016: Jornal Brasileiro de Pneumologia: Publicaça̋o Oficial da Sociedade Brasileira de Pneumologia e Tisilogia
https://www.readbyqxmd.com/read/27318212/protein-creatinine-index-a-possible-predictor-of-nephropathy-in-hypertensives-in-northern-india
#18
Akif Ahsan, Kush Manna, Gajraj S Yadav, Arsalan Moinuddin, Aquil Ahmad, R C Gupta, Sana Alam
BACKGROUND: >33% of the hypertensive Indians develops nephropathy. Proteinuria is an early indicator of nephropathy. Gold standard for determining proteinuria is 24-hour urinary protein excretion which is a troublesome task with poor patient compliance. Protein creatinine index (PCI) in a random urine sample has been advocated by some researchers as an alternative approach. Aim of this study was to evaluate the association of PCI with the severity and duration of hypertension, in North Indian population...
September 1, 2016: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/27221741/in-situ-tuning-of-gold-nanorod-plasmon-through-oxidative-cyanide-etching
#19
Aquiles Carattino, Saumyakanti Khatua, Michel Orrit
Single gold nanorods exhibit great opportunities for bio-sensing, enhanced spectroscopies and photothermal therapy. A key property of these particles is the surface plasmon resonance, that is strongly dependent on their shape. Methods for tuning this resonance after the synthesis of the particles are of great interest for many applications. In this work we show that, through very well known chemistry between gold atoms and cyanide ions, it is possible to tune the surface plasmon of single 25 × 50 nm rods by more than 100 nm towards longer wavelengths...
June 21, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27172851/interlocutor-differential-effects-on-the-expressive-language-skills-of-spanish-speaking-english-learners
#20
Raúl Rojas, Aquiles Iglesias, Ferenc Bunta, Brian Goldstein, Claude Goldenberg, Leslie Reese
PURPOSE: The primary objective of this study was to investigate the relationship between language use with different interlocutors, maternal education level and the expressive language skills of US English learners (ELs) in Spanish and English. METHOD: Two hundred and twenty-four Spanish-speaking ELs in kindergarten provided narrative language samples in Spanish and English. Parents completed a questionnaire of maternal education level and language use with parents, older siblings and peers...
April 2016: International Journal of Speech-language Pathology
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