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https://www.readbyqxmd.com/read/28179116/immunogenicity-of-amino-acids-1-150-of-streptococcus-gapc-displayed-on-the-surface-of-escherichia-coli
#1
Baifen Song, Xijing Yang, Hunan Sun, Liquan Yu, Jinzhu Ma, Zhijun Wu, Yudong Cui
Streptococcus is one of the main pathogens that cause bovine mastitis. They includes into S.agalactiae, S.dysgalactiae, and S.uberis. The GapC protein is a virulence factor that is expressed on the surface of Streptococcus species. GapC is highly antigenic and immunization with GapC confers cross-protection against all three species. Our previous data showed that amino acids 1-150 of GapC (GapC1-150) of S. dysgalactiae conferred similar immunoprotection compared to full-length GapC. Thus, the present study aimed to construct a recombinant Escherichia coli XL1-Blue strain that displayed GapC1-150 on its surface, and to investigate the immunogenicity of the surface-localized GapC1-150...
February 4, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28158302/host-cell-interactions-of-outer-membrane-vesicle-associated-virulence-factors-of-enterohemorrhagic-escherichia-coli-o157-intracellular-delivery-trafficking-and-mechanisms-of-cell-injury
#2
Martina Bielaszewska, Christian Rüter, Andreas Bauwens, Lilo Greune, Kevin-André Jarosch, Daniel Steil, Wenlan Zhang, Xiaohua He, Roland Lloubes, Angelika Fruth, Kwang Sik Kim, M Alexander Schmidt, Ulrich Dobrindt, Alexander Mellmann, Helge Karch
Outer membrane vesicles (OMVs) are important tools in bacterial virulence but their role in the pathogenesis of infections caused by enterohemorrhagic Escherichia coli (EHEC) O157, the leading cause of life-threatening hemolytic uremic syndrome, is poorly understood. Using proteomics, electron and confocal laser scanning microscopy, immunoblotting, and bioassays, we investigated OMVs secreted by EHEC O157 clinical isolates for virulence factors cargoes, interactions with pathogenetically relevant human cells, and mechanisms of cell injury...
February 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28128530/the-effects-of-low-level-laser-therapy-on-both-hiv-1-infected-and-uninfected-tzm-bl-cells
#3
Masixole Yvonne Lugongolo, Sello Lebohang Manoto, Saturnin Ombinda-Lemboumba, Malik Maaza, Patience Mthunzi-Kufa
Human immunodeficiency virus (HIV-1) infection remains a major health problem despite the use of highly active antiretroviral therapy (HAART), which has greatly reduced mortality rates. Due to the unavailability of an effective vaccine and treatment that would completely eradicate the virus in infected individuals, the quest for new therapies continues. Low level laser therapy (LLLT) involves the exposure of cells to low levels of red or infrared light. LLLT has been widely used in different medical conditions, but not in HIV-1 infection...
January 27, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28124198/laser-assisted-topical-corticosteroid-delivery-for-the-treatment-of-keloids
#4
Ji Hye Park, Ji Young Chun, Jong Hee Lee
Laser-assisted drug delivery has generated intense interest. The objectives of this study are to evaluate the clinical benefit of laser-assisted corticosteroid delivery and to compare this technique to corticosteroid intralesional injection, a standard treatment for keloids. Patients with keloids on the left shoulder after BCG vaccination were enrolled in this study. The entire lesion was first treated with an ablative fractional erbium-YAG laser. After this treatment, the lesion was divided into two halves...
January 26, 2017: Lasers in Medical Science
https://www.readbyqxmd.com/read/28117172/skin-vaccination-via-fractional-infrared-laser-ablation-optimization-of-laser-parameters-and-adjuvantation
#5
Sandra Scheiblhofer, Anna Strobl, Veronika Hoepflinger, Theresa Thalhamer, Martin Steiner, Josef Thalhamer, Richard Weiss
BACKGROUND: Methods to deliver an antigen into the skin in a painless, defined, and reproducible manner are essential for transcutaneous immunization (TCI). Here, we employed an ablative fractional infrared laser (P.L.E.A.S.E. Professional) to introduce clinically relevant vaccines into the skin. To elicit the highest possible antibody titers with this system, we optimized different laser parameters, such as fluence and pore number per area, and tested various adjuvants. METHODS: BALB/c mice were immunized with Hepatitis B surface antigen (HBsAg) by laser-microporation...
January 20, 2017: Vaccine
https://www.readbyqxmd.com/read/28043501/comparative-proteomic-analysis-of-sequential-isolates-of-mycobacterium-tuberculosis-sensitive-and-resistant-beijing-type-from-a-patient-with-pulmonary-tuberculosis
#6
Amit Singh, Anil Kumar Gupta, Krishnamoorthy Gopinath, Divakar Sharma, Prashant Sharma, Deepa Bisht, Pawan Sharma, Sarman Singh
AIM & OBJECTIVE: In India, tuberculosis (TB) is a foremost health problem, and the emergence of multidrug-resistant (MDR) and extensively drug resistant (XDR) strains of Mycobacterium tuberculosis (M. tuberculosis) has further complicated the situation. Although various mechanisms have been proposed to elucidate the emergence of resistance, our knowledge remains insufficient. The formation of a very complex network and drugs of proteins are countered by their efflux/modification or target over-expression/modification...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28028922/n-dihydrogalactochitosan-as-a-potent-immune-activator-for-dendritic-cells
#7
Ahmed El-Hussein, Samuel S K Lam, Joseph Raker, Wei R Chen, Michael R Hamblin
Immunotherapy has become one of the fastest growing areas of cancer research. A promising in situ autologous cancer vaccine (inCVAX) uses a novel immune activator, N-dihydrogalactochitosan (GC), that possesses the ability to stimulate dendritic cells (DC). inCVAX is a combination treatment procedure involving treatment of the tumor with a thermal near-infrared laser to liberate whole cell tumor antigens, followed by injection of GC (a glucosamine polymer with galactose attached to the amino groups) into the treated tumor thereby inducing a systemic anti-tumor immune response...
December 28, 2016: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/27959277/endothelial-dysfunction-may-play-a-key-role-in-keloid-and-hypertrophic-scar-pathogenesis-keloids-and-hypertrophic-scars-may-be-vascular-disorders
#8
Rei Ogawa, Satoshi Akaishi
Keloids and hypertrophic scars are fibroproliferative disorders (FPDs) of the skin that result from abnormal healing of injured or irritated skin. They can be called pathological or inflammatory scars. Common causes are trauma, burn, surgery, vaccination, skin piercing, folliculitis, acne, and herpes zoster infection. The pathogenesis of these scars clearly involves local conditions such as delayed wound healing, wound depth, and the tension of the skin around the scars. Scar severity is also shaped by interactions between these local factors and genetic and systemic factors such as hypertension and sex hormones...
November 2016: Medical Hypotheses
https://www.readbyqxmd.com/read/27930728/profiling-and-identification-of-novel-immunogenic-proteins-of-staphylococcus-hyicus-zc-4-by-immunoproteomic-assay
#9
Lei Wang, Zhi-Wei Wu, Yan Li, Jian-Guo Dong, Le-Yi Zhang, Peng-Shuai Liang, Yan-Ling Liu, Ya-Hua Zhao, Chang-Xu Song
Staphylococcus hyicus has caused great losses in the swine industry by inducing piglet exudative epidermitis (EE), sow mastitis, metritis, and other diseases and is a threat to human health. The pathogenesis of EE, sow mastitis, and metritis involves the interaction between the host and virulent protein factors of S. hyicus, however, the proteins that interact with the host, especially the host immune system, are unclear. In the present study, immunoproteomics was used to screen the immunogenic proteins of S...
2016: PloS One
https://www.readbyqxmd.com/read/27919900/n-acetyl-l-cysteine-and-cysteamine-as-new-strategies-against-mixed-biofilms-of-nonencapsulated-streptococcus-pneumoniae-and-nontypeable-haemophilus-influenzae
#10
Mirian Domenech, Ernesto García
Acute otitis media, a polymicrobial disease of the middle ear cavity of children, is a significant public health problem worldwide. It is most frequently caused by encapsulated Streptococcus pneumoniae and nontypeable Haemophilus influenzae, although the widespread use of pneumococcal conjugate vaccines is apparently producing an increase in the carriage of nonencapsulated S. pneumoniae Frequently, pneumococci and H. influenzae live together in the human nasopharynx, forming a self-produced biofilm. Biofilms present a global medical challenge since the inherent antibiotic resistance of their producers demands the use of large doses of antibiotics over prolonged periods...
February 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27833475/noninvasive-transdermal-vaccination-using-hyaluronan-nanocarriers-and-laser-adjuvant
#11
Ki Su Kim, Hyemin Kim, Yunji Park, Won Ho Kong, Seung Woo Lee, Sheldon J J Kwok, Sei Kwang Hahn, Seok Hyun Yun
Vaccines are commonly administered by injection using needles. Although transdermal microneedles are less-invasive promising alternatives, needle-free topical vaccination without involving physical damage to the natural skin barrier is still sought after as it can further reduce needle-induced anxiety and simply administration. However, this long-standing goal has been elusive since the intact skin is impermeable to most macromolecules. Here, we show an efficient, non-invasive transdermal vaccination in mice by employing two key innovations: first, the use of hyaluronan (HA) as vaccine carriers and, second, non-ablative laser adjuvants...
April 19, 2016: Advanced Functional Materials
https://www.readbyqxmd.com/read/27814617/tumor-abolition-and-antitumor-immunostimulation-by-physico-chemical-tumor%C3%A2-ablation
#12
Yona Keisari
Tumor ablation by thermal, chemical and radiological sources has received substantial attention for the treatment of many localized malignancies. The primary goal of most ablation procedures is to eradicate all viable malignant cells within a designated target volume through the application of energy or chemicals. Methods such as radiotherapy, chemical and biological ablation, photodynamic therapy, cryoablation, high-temperature ablation (radiofrequency, microwave, laser, and ultrasound), and electric-based ablation have been developed for focal malignancies...
January 1, 2017: Frontiers in Bioscience (Landmark Edition)
https://www.readbyqxmd.com/read/27778355/skin-pre-ablation-and-laser-assisted-microjet-injection-for-deep-tissue-penetration
#13
Hun-Jae Jang, Seonggu Yeo, Jack J Yoh
BACKGROUND AND AIMS: For conventional needless injection, there still remain many unresolved issues such as the potential for cross-contamination, poor reliability of targeted delivery dose, and significantly painstaking procedures. As an alternative, the use of microjets generated with Er:YAG laser for delivering small doses with controlled penetration depths has been reported. In this study, a new system with two stages is evaluated for effective transdermal drug delivery. First, the skin is pre-ablated to eliminate the hard outer layer and second, laser-driven microjet penetrates the relatively weaker and freshly exposed epidermis...
October 25, 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/27767031/photothermal-therapy-with-immune-adjuvant-nanoparticles-together-with-checkpoint-blockade-for-effective-cancer-immunotherapy
#14
Qian Chen, Ligeng Xu, Chao Liang, Chao Wang, Rui Peng, Zhuang Liu
A therapeutic strategy that can eliminate primary tumours, inhibit metastases, and prevent tumour relapses is developed herein by combining adjuvant nanoparticle-based photothermal therapy with checkpoint-blockade immunotherapy. Indocyanine green (ICG), a photothermal agent, and imiquimod (R837), a Toll-like-receptor-7 agonist, are co-encapsulated by poly(lactic-co-glycolic) acid (PLGA). The formed PLGA-ICG-R837 nanoparticles composed purely by three clinically approved components can be used for near-infrared laser-triggered photothermal ablation of primary tumours, generating tumour-associated antigens, which in the presence of R837-containing nanoparticles as the adjuvant can show vaccine-like functions...
October 21, 2016: Nature Communications
https://www.readbyqxmd.com/read/27661656/committee-opinion-no-675-management-of-vulvar-intraepithelial-neoplasia
#15
(no author information available yet)
Vulvar intraepithelial neoplasia (VIN) is an increasingly common problem, particularly among women in their 40s. Although spontaneous regression has been reported, VIN should be considered a premalignant condition. Immunization with the quadrivalent or 9-valent human papillomavirus vaccine, which is effective against human papillomavirus genotypes 6, 11, 16, and 18, and 6, 11, 16, 18, 31, 33, 45, 52, and 58, respectively, has been shown to decrease the risk of vulvar high-grade squamous intraepithelial lesion (HSIL) (VIN usual type) and should be recommended for girls aged 11-12 years with catch-up through age 26 years if not vaccinated in the target age...
2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27661648/committee-opinion-no-675-summary-management-of-vulvar-intraepithelial-neoplasia
#16
(no author information available yet)
Vulvar intraepithelial neoplasia (VIN) is an increasingly common problem, particularly among women in their 40s. Although spontaneous regression has been reported, VIN should be considered a premalignant condition. Immunization with the quadrivalent or 9-valent human papillomavirus vaccine, which is effective against human papillomavirus genotypes 6, 11, 16, and 18, and 6, 11, 16, 18, 31, 33, 45, 52, and 58, respectively, has been shown to decrease the risk of vulvar high-grade squamous intraepithelial lesion (HSIL) (VIN usual type) and should be recommended for girls aged 11-12 years with catch-up through age 26 years if not vaccinated in the target age...
October 2016: Obstetrics and Gynecology
https://www.readbyqxmd.com/read/27450536/identification-and-characterization-of-an-immunogenic-antigen-enolase-2-among-excretory-secretory-antigens-esa-of-toxoplasma-gondii
#17
Wei Jiang, Jun-Xin Xue, Ying-Chun Liu, Tao Li, Xian-Gan Han, Shao-Hui Wang, Yong-Jun Chen, Jingjing Qi, Sheng-Qing Yu, Quan Wang
An immunogenic protein, enolase 2, was identified among the secreted excretory/secretory antigens (ESAs) from Toxoplasma gondii strain RH using immunoproteomics based on matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Enolase 2 was cloned, sequenced, and heterologously expressed. BLAST analysis revealed 75-96% similarity with enolases from other parasites. Immunoblotting demonstrated good immunoreactivity of recombinant T. gondii enolase (Tg-enolase 2) to T. gondii-infected animal serum...
November 2016: Protein Expression and Purification
https://www.readbyqxmd.com/read/27417316/proteomic-analysis-of-drug-resistant-mycobacterium-tuberculosis-by-one-dimensional-gel-electrophoresis-and-charge-chromatography
#18
Shamsi Yari, Alireza Hadizadeh Tasbiti, Mostafa Ghanei, Mohammad Ali Shokrgozar, Abolfazl Fateh, Reza Mahdian, Fatemeh Yari, Ahmadreza Bahrmand
Multidrug-resistant tuberculosis (MDR-TB) is a form of TB caused by Mycobacterium tuberculosis (M. tuberculosis) that do not respond to, at least, isoniazid and rifampicin, the two most powerful, first-line (or standard) anti-TB drugs. Novel intervention strategies for eliminating this disease were based on finding proteins that can be used for designing new drugs or new and reliable kits for diagnosis. The aim of this study was to compare the protein profiles of MDR-TB with sensitive isolates. Proteomic analysis of M...
January 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/27367671/fourier-transform-mass-spectrometry-and-nuclear-magnetic-resonance-analysis-for-the-rapid-and-accurate-characterization-of-hexacosanoylceramide
#19
Charles W Ross, William J Simonsick, Michael J Bogusky, Recep W Celikay, James P Guare, Randall C Newton
Ceramides are a central unit of all sphingolipids which have been identified as sites of biological recognition on cellular membranes mediating cell growth and differentiation. Several glycosphingolipids have been isolated, displaying immunomodulatory and anti-tumor activities. These molecules have generated considerable interest as potential vaccine adjuvants in humans. Accurate analyses of these and related sphingosine analogues are important for the characterization of structure, biological function, and metabolism...
June 28, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27321476/potential-of-nanoparticles-for-allergen-specific-immunotherapy-use-of-silica-nanoparticles-as-vaccination-platform
#20
Sandra Scheiblhofer, Yoan Machado, Andrea Feinle, Josef Thalhamer, Nicola Hüsing, Richard Weiss
INTRODUCTION: Allergen-specific immunotherapy is the only curative approach for the treatment of allergies. There is an urgent need for improved therapies, which increase both, efficacy and patient compliance. Novel routes of immunization and the use of more advanced vaccine platforms have gained heightened interest in this field. AREAS COVERED: The current status of allergen-specific immunotherapy is summarized and novel routes of immunization and their challenges in the clinics are critically discussed...
July 6, 2016: Expert Opinion on Drug Delivery
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