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https://www.readbyqxmd.com/read/29037785/novel-bilayer-dissolving-microneedle-arrays-with-concentrated-plga-nano-microparticles-for-targeted-intradermal-delivery-proof-of-concept
#1
Lalit K Vora, Ryan F Donnelly, Eneko Larraneta, Patricia González-Vázquez, Raghu Raj Singh Thakur, Pradeep R Vavia
Polymeric microneedle (MN) arrays continue to receive growing attention due to their ability to bypass the skin's stratum corneum barrier in a minimally-invasive fashion and achieve enhanced transdermal drug delivery and "targeted" intradermal vaccine administration. In this research work, we fabricated biodegradable bilayer MN arrays containing nano - microparticles for targeted and sustained intradermal drug delivery. For this study, model drug (vitamin D3, VD3)-loaded PLGA nano- and microparticles (NMP) were prepared by a single emulsion solvent evaporation method with 72...
October 13, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28946696/immunogenicity-of-egfp-marked-recombinant-lactobacillus-casei-against-transmissible-gastroenteritis-virus-and-porcine-epidemic-diarrhea-virus
#2
Meiling Yu, Li Wang, Sunting Ma, Xiaona Wang, Yusai Wang, Ya Xiao, Yanping Jiang, Xinyuan Qiao, Lijie Tang, Yigang Xu, Yijing Li
Porcine transmissible gastroenteritis virus (TGEV) and porcine epidemic diarrhea virus (PEDV) are the causative agents of highly fatal acute diarrhea in pigs, resulting in enormous losses in the pig industry worldwide. To develop an effective bivalent oral vaccine against TGEV and PEDV infection, the D antigenic site of the TGEV spike (S) protein and the major antigen site (core neutralizing epitope-COE) of the PEDV S protein were used as immunogens, and the enhanced green fluorescent protein (eGFP) gene was used as a reporter to construct genetically engineered Lactobacillus casei rLpPG(F)-T7g10-eGFP-6D-COE...
September 25, 2017: Viruses
https://www.readbyqxmd.com/read/28919557/synergistic-effects-of-dendritic-cell-targeting-and-laser-microporation-on-enhancing-epicutaneous-skin-vaccination-efficacy
#3
Yoan Machado, Sanne Duinkerken, Veronika Hoepflinger, Melissa Mayr, Evgeniia Korotchenko, Almedina Kurtaj, Isabel Pablos, Markus Steiner, Angelika Stoecklinger, Joyce Lübbers, Maximillian Schmid, Uwe Ritter, Sandra Scheiblhofer, Michael Ablinger, Verena Wally, Sarah Hochmann, Anna M Raninger, Dirk Strunk, Yvette van Kooyk, Josef Thalhamer, Richard Weiss
Due to its unique immunological properties, the skin is an attractive target tissue for allergen-specific immunotherapy. In our current work, we combined a dendritic cell targeting approach with epicutaneous immunization using an ablative fractional laser to generate defined micropores in the upper layers of the skin. By coupling the major birch pollen allergen Bet v 1 to mannan from S. cerevisiae via mild periodate oxidation we generated hypoallergenic Bet-mannan neoglycoconjugates, which efficiently targeted CD14(+) dendritic cells and Langerhans cells in human skin explants...
September 14, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28900429/plasma-derived-polyreactive-secretory-like-iga-and-igm-opsonizing-salmonella-enterica-typhimurium-reduces-invasion-and-gut-tissue-inflammation-through-agglutination
#4
Gilles Bioley, Justine Monnerat, Marius Lötscher, Cédric Vonarburg, Adrian Zuercher, Blaise Corthésy
Due to the increasing emergence of antibiotic-resistant strains of enteropathogenic bacteria, development of alternative treatments to fight against gut infections is a major health issue. While vaccination requires that a proper combination of antigen, adjuvant, and delivery route is defined to elicit protective immunity at mucosae, oral delivery of directly active antibody preparations, referred to as passive immunization, sounds like a valuable alternative. Along the gut, the strategy suffers, however, from the difficulty to obtain sufficient amounts of antibodies with the appropriate specificity and molecular structure for mucosal delivery...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28859241/mir-125b-1-and-mir-378a-are-predictive-biomarkers-for-the-efficacy-of-vaccine-treatment-against-colorectal-cancer
#5
Hironori Tanaka, Shoichi Hazama, Michihisa Iida, Ryouichi Tsunedomi, Hiroko Takenouchi, Masao Nakajima, Yukio Tokumitsu, Shinsuke Kanekiyo, Yoshitaro Shindo, Shinobu Tomochika, Yoshihiro Tokuhisa, Kazuhiko Sakamoto, Nobuaki Suzuki, Shigeru Takeda, Shigeru Yamamoto, Shigefumi Yoshino, Tomio Ueno, Yoshihiko Hamamoto, Yusuke Fujita, Hiroaki Tanaka, Ko Tahara, Ryoichi Shimizu, Kiyotaka Okuno, Koji Fujita, Masahiko Kuroda, Yusuke Nakamura, Hiroaki Nagano
Many clinical trials of peptide vaccines have been conducted. However, these vaccines have provided clinical benefits in only a small fraction of patients. The purpose of the present study was to explore microRNAs (miRNAs) as novel predictive biomarkers for the efficacy of vaccine treatment against colorectal cancer. First, we carried out microarray analysis of pretreatment cancer tissues in a phase I study, in which peptide vaccines alone were given. Candidate miRNAs were selected by comparison of the better prognosis group with the poorer prognosis group...
November 2017: Cancer Science
https://www.readbyqxmd.com/read/28840259/treatment-outcomes-of-recurrent-respiratory-papillomatosis-retrospective-analysis-of-juvenile-and-adult-cases
#6
V-A Papaioannou, A Lux, S Voigt-Zimmermann, C Arens
BACKGROUND: Recurrent respiratory papillomatosis (RRP) is a rare, chronic disease of viral etiology which is characterized by multiple, recurrent growth of papillomas in the aerodigestive tract. MATERIALS AND METHODS: The surgical outcomes and the recurrence rates of 106 patients with RRP of the larynx were analyzed. The patients were treated at the University of Magdeburg between 1983 and 2014. The surgical outcomes of conventional and laser surgery regarding time to relapse and complications were compared...
August 24, 2017: HNO
https://www.readbyqxmd.com/read/28776443/exploring-the-immunopotentiation-of-chinese-yam-polysaccharide-poly-lactic-co-glycolic-acid-nanoparticles-in-an-ovalbumin-vaccine-formulation-in-vivo
#7
Li Luo, Tao Qin, Yifan Huang, Sisi Zheng, Ruonan Bo, Zhenguang Liu, Jie Xing, Yuanliang Hu, Jiaguo Liu, Deyun Wang
Biocompatible and biodegradable poly(lactic-co-glycolic acid) (PLGA) has been approved by the US Food and Drug Administration and has frequently been used to develop potential vaccine delivery systems. The immunoregulation and immunopotentiation of Chinese yam polysaccharide (CYP) have been widely demonstrated. In the current study, cell uptake mechanisms in dendritic cells (DCs) were monitored in vitro using confocal laser scanning microscopy, transmission electron microscopy, and flow cytometry. To study a CYP-PLGA nanoparticle-adjuvanted delivery system, CYP and ovalbumin (OVA) were encapsulated in PLGA nanoparticles (CYPPs) to act as a vaccine, and the formulation was tested in immunized mice...
November 2017: Drug Delivery
https://www.readbyqxmd.com/read/28755834/immunogenicity-of-adenovirus-vaccines-expressing-the-pcv2-capsid-protein-in-pigs
#8
Delong Li, Qian Du, Bin Wu, Juejun Li, Lingling Chang, Xiaomin Zhao, Yong Huang, Dewen Tong
Porcine circovirus type 2 (PCV2) is the main pathogen of porcine circovirus associated disease (PCVAD), causing great economic losses in pig industry. In previous study, we constructed adenovirus vector vaccines expressing PCV2 Cap either modified with Intron A and WPRE, or CD40L and GMCSF, and evaluated all of these vaccines in mice and in pigs. Although Ad-A-C-W and Ad-CD40L-Cap-GMCSF could induce stronger immune responses than Ad-Cap, neither of them was better than commercial inactivated vaccine PCV2 SH-strain...
July 26, 2017: Vaccine
https://www.readbyqxmd.com/read/28746382/gelatin-nanoparticles-enhance-delivery-of-hepatitis-c-virus-recombinant-ns2-gene
#9
Salwa Sabet, Marina A George, Haidan M El-Shorbagy, Heba Bassiony, Khaled Y Farroh, Tareq Youssef, Taher A Salaheldin
BACKGROUND: Development of an effective non-viral vaccine against hepatitis C virus infection is of a great importance. Gelatin nanoparticles (Gel.NPs) have an attention and promising approach as a viable carrier for delivery of vaccine, gene, drug and other biomolecules in the body. AIM OF WORK: The present study aimed to develop stable Gel.NPs conjugated with nonstructural protein 2 (NS2) gene of Hepatitis C Virus genotype 4a (HCV4a) as a safe and an efficient vaccine delivery system...
2017: PloS One
https://www.readbyqxmd.com/read/28733985/lalf32-51-e7-a-hpv-16-therapeutic-vaccine-candidate-forms-protein-body-like-structures-when-expressed-in-nicotiana-benthamiana-leaves
#10
Romana J R Yanez, Renate Lamprecht, Milaid Granadillo, Isis Torrens, Elsa Arcalís, Eva Stöger, Edward P Rybicki, Inga I Hitzeroth
High-risk human papillomaviruses (HPVs) cause cervical cancer, and while there are good prophylactic vaccines on the market, these are ineffective against established infections, creating a clear need for therapeutic vaccines. The HPV E7 protein is one of the essential oncoproteins for the onset and maintenance of malignancy and is therefore an ideal therapeutic vaccine target. We fused the HPV-16 E7 protein to the Limulus polyphemus antilipopolysaccharide factor (LALF32-51 ), a small hydrophobic peptide that can penetrate cell membranes and that has immunomodulatory properties...
July 22, 2017: Plant Biotechnology Journal
https://www.readbyqxmd.com/read/28722543/comparison-of-ovalbumin-and-ovalbumin-epitope-peptide-for-transdermal-delivery-and-vaccination-mediated-by-the-photothermal-effect-of-gold-nanorods
#11
Aung Thu Haine, Yuji Miyamoto, Taishi Higashi, Keiichi Motoyama, Hidetoshi Arima, Takuro Niidome
Transdermal protein delivery is a powerful and attractive method for protein therapy and dermal vaccination compared with other administrations. However, this delivery method is restricted by the low permeability of the stratum corneum (SC), a hydrophobic barrier that restricts the entry of hydrophilic molecules such as proteins. In this study, we developed an improved gel patch system carrying ovalbumin and ovalbumin epitope peptide, and then compared their permeability into the skin. First, the gel patch was placed on mouse skin to allow contact with the polymer coated gold nanorods and then irradiated by a continuous-wave laser...
July 26, 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28710539/-treatment-outcomes-of-recurrent-respiratory-papillomatosis-retrospective-analysis-of-juvenile-and-adult-cases-german-version
#12
V-A Papaioannou, A Lux, S Voigt-Zimmermann, C Arens
BACKGROUND: Recurrent respiratory papillomatosis (RRP) is a rare, chronic disease of viral etiology which is characterized by multiple, recurrent growth of papillomas in the aerodigestive tract. MATERIALS AND METHODS: The surgical outcomes and the recurrence rates of 106 patients with RRP of the larynx were analyzed. The patients were treated at the University of Magdeburg between 1983 and 2014. The surgical outcomes of conventional and laser surgery regarding time to relapse and complications were compared...
November 2017: HNO
https://www.readbyqxmd.com/read/28710250/near-infrared-1064-nm-laser-modulates-migratory-dendritic-cells-to-augment-the-immune-response-to-intradermal-influenza-vaccine
#13
Kaitlyn Morse, Yoshifumi Kimizuka, Megan P K Chan, Mai Shibata, Yusuke Shimaoka, Shu Takeuchi, Benjamin Forbes, Christopher Nirschl, Binghao Li, Yang Zeng, Roderick T Bronson, Wataru Katagiri, Ayako Shigeta, Ruxandra F Sîrbulescu, Huabiao Chen, Rhea Y Y Tan, Kosuke Tsukada, Timothy Brauns, Jeffrey Gelfand, Ann Sluder, Joseph J Locascio, Mark C Poznansky, Niroshana Anandasabapathy, Satoshi Kashiwagi
Brief exposure of skin to near-infrared (NIR) laser light has been shown to augment the immune response to intradermal vaccination and thus act as an immunologic adjuvant. Although evidence indicates that the NIR laser adjuvant has the capacity to activate innate subsets including dendritic cells (DCs) in skin as conventional adjuvants do, the precise immunological mechanism by which the NIR laser adjuvant acts is largely unknown. In this study we sought to identify the cellular target of the NIR laser adjuvant by using an established mouse model of intradermal influenza vaccination and examining the alteration of responses resulting from genetic ablation of specific DC populations...
August 15, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28686409/structural-characterization-of-biofunctionalized-gold-nanoparticles-by-ultrahigh-resolution-mass-spectrometry
#14
Simone Nicolardi, Yuri E M van der Burgt, Jeroen D C Codée, Manfred Wuhrer, Cornelis H Hokke, Fabrizio Chiodo
Biofunctionalized gold nanoparticles (AuNPs) enable innovative translational research and development in biomedicine. Biomolecules such as peptides, proteins, lipids, and carbohydrates can be assembled onto AuNPs to yield nanomaterials with unique properties for applications in imaging, photothermal therapy, vaccination strategies, and drug delivery. The characterization of functionalized AuNPs still remains an analytical challenge that normally requires the combination of multiple techniques. Laser desorption/ionization (LDI) and matrix-assisted LDI (MALDI) have been applied successfully in combination with time-of-flight (TOF) mass spectrometry (MS) for the analysis of the surface chemistry of AuNPs functionalized with synthetic ligands, however only for ligands with a molecular mass limited to 1000 Da...
August 22, 2017: ACS Nano
https://www.readbyqxmd.com/read/28683213/proteomics-of-culture-filtrate-of-prevalent-mycobacterium-tuberculosis-strains-2d-page-map-and-maldi-tof-ms-analysis
#15
Gavish Kumar, Hari Shankar, Divakar Sharma, Prashant Sharma, Deepa Bisht, Vishwa M Katoch, Beenu Joshi
Although diverse efforts have been done to identify biomarkers for control of tuberculosis using laboratory strain Mycobacterium tuberculosis H37Rv, the disease still poses a threat to mankind. There are many emerging M. tuberculosis strains, and proteomic profiling of these strains might be important to find out potential targets for diagnosis and/or prevention of tuberculosis. We evaluated the comparative proteomic profiling of culture filtrate (CF) proteins from prevalent M. tuberculosis strains (Central Asian or Delhi type; CAS1_Del, East African-Indian; EAI-3 and Beijing family) by 2D polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry...
July 1, 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28677147/looking-beyond-human-papillomavirus-hpv-genotype-16-and-18-defining-hpv-genotype-distribution-in-cervical-cancers-in-australia-prior-to-vaccination
#16
Julia M L Brotherton, Sepehr N Tabrizi, Samuel Phillips, Jan Pyman, Alyssa M Cornall, Neil Lambie, Lyndal Anderson, Margaret Cummings, Diane Payton, James P Scurry, Marsali Newman, Raghwa Sharma, Marion Saville, Suzanne M Garland
Australia has implemented a high-coverage HPV vaccination program but has not, to date, established the distribution of HPV types that occur in cervical cancers in Australia. This information is important for determining the potential for cervical cancer prevention with both current and broader spectrum HPV vaccines. We analysed 847 cervical cancers diagnosed 2005 to 2015 in tertiary centres in the three most populous Australian states with resolution of specimens containing multiple HPV types using laser-capture microdissection...
October 15, 2017: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/28637906/anogenital-warts-and-other-hpv-associated-anogenital-lesions-in-the-hiv-positive-patient-a-systematic-review-and-meta-analysis-of-the-efficacy-and-safety-of-interventions-assessed-in-controlled-clinical-trials
#17
Ricardo Niklas Werner, Lukas Westfechtel, Corinna Dressler, Alexander Nast
OBJECTIVES: Anogenital warts (AGW, condylomata acuminata) and intraepithelial neoplasia (IEN) do not only impact health and social well-being, they are also associated with considerable costs for the healthcare systems. Immunocompromised and HIV-positive patients carry the highest epidemiological burden of human papillomavirus (HPV) infection and comprise a population specifically susceptible to treatment failures and recurrences. This systematic review aimed at identifying and appraising the available evidence from controlled studies of interventions for the treatment of AGW and IEN in immunocompromised patients...
June 21, 2017: Sexually Transmitted Infections
https://www.readbyqxmd.com/read/28629460/interaction-between-pasteurella-multocida-b-2-and-its-derivatives-with-bovine-aortic-endothelial-cell-baec
#18
Nuriqmaliza M Kamal, M Zamri-Saad, Mas Jaffri Masarudin, Sarah Othman
BACKGROUND: Pasteurella multocida B:2 causes bovine haemorrhagic septicaemia (HS), leading to rapid fatalities in cattle and buffaloes. An attenuated derivative of P. multocida B:2 GDH7, was previously constructed through mutation of the gdhA gene and proved to be an effective live attenuated vaccine for HS. Currently, only two potential live attenuated vaccine candidates for HS are being reported; P. multocida B:2 GDH7 and P. multocida B:2 JRMT12. This study primarily aims to investigate the potential of P...
June 19, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28600224/enhancement-of-transdermal-protein-delivery-by-photothermal-effect-of-gold-nanorods-coated-on-polysaccharide-based-hydrogel
#19
Aung Thu Haine, Yuki Koga, Yuta Hashimoto, Taishi Higashi, Keiichi Motoyama, Hidetoshi Arima, Takuro Niidome
Transdermal protein delivery is a useful and attractive method for protein therapy and dermal vaccination. However, this delivery method is restricted by the low permeability of the stratum corneum. The purpose of this study was to develop a transdermal delivery system for enhancement of protein permeability into the skin. First, we prepared a transparent gel patch made of polysaccharides with gold nanorods on the gel surface and fluorescein isothiocyanate-modified ovalbumin (FITC-OVA) inside. Next, the gel patch was placed on mouse skin to allow contact with the coated gold nanorods, and irradiated by a continuous-wave laser...
October 2017: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/28407312/nasopharyngeal-papillomas-treated-with-co2-laser-and-human-papillomavirus-vaccination
#20
Yi Cai, Valeria S Merea, Andrew H Lee, Gloria H Su, Salvatore M Caruana
This case report describes the use of CO2 laser and quadrivalent human papillomavirus (HPV) vaccination in two patients with nasopharyngeal HPV11-positive recurrent respiratory papillomas (RRP). These patients initially underwent CO2 laser excision but developed recurrent lesions and underwent HPV vaccination as adjunct therapy. The recurrent lesions shrank after vaccination and were again excised with CO2 laser. Subsequently, these patients had no recurrence of lesions on long-term follow-up at 33 months or 6 years after surgery...
October 2017: Laryngoscope
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