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https://www.readbyqxmd.com/read/29670088/a-cancer-vaccine-mediated-postoperative-immunotherapy-for-recurrent-and-metastatic-tumors
#1
Tingting Wang, Dangge Wang, Haijun Yu, Bing Feng, Fangyuan Zhou, Hanwu Zhang, Lei Zhou, Shi Jiao, Yaping Li
Vaccines to induce effective and sustained antitumor immunity have great potential for postoperative cancer therapy. However, a robust cancer vaccine simultaneously eliciting tumor-specific immunity and abolishing immune resistance continues to be a challenge. Here we present a personalized cancer vaccine (PVAX) for postsurgical immunotherapy. PVAX is developed by encapsulating JQ1 (a BRD4 inhibitor) and indocyanine green (ICG) co-loaded tumor cells with a hydrogel matrix. Activation of PVAX by 808 nm NIR laser irradiation significantly inhibits the tumor relapse by promoting the maturation of dendritic cells and eliciting tumor infiltration of cytotoxic T lymphocytes...
April 18, 2018: Nature Communications
https://www.readbyqxmd.com/read/29660407/application-of-water-soluble-polyvinyl-alcohol-based-film-patches-on-laser-microporated-skin-facilitates-intradermal-macromolecule-and-nanoparticle-delivery
#2
Laura Engelke, Gerhard Winter, Julia Engert
The intradermal delivery of biologics has long been recognized as attractive approach for cutaneous immunotherapy, particularly vaccination. Although intradermal (i.d.) or subcutaneous (s.c.) injection provide reproducible dosing and good cost- and delivery efficiency, the major objective to avoid sharps and the need for enhanced storage stability have renewed the interest in alternative needle-free delivery strategies. This study presents a new concept for the delivery of macromolecules and nanoparticles to viable skin layers with a high density of professional antigen-presenting cells (APCs)...
April 13, 2018: European Journal of Pharmaceutics and Biopharmaceutics
https://www.readbyqxmd.com/read/29594918/squamous-cell-papillomatosis-in-the-setting-of-recurrent-respiratory-papillomatosis
#3
Pasha L Bentley, Michael J Coulter, Brenda L Nelson
A 23 year old male presented to the Otolaryngology clinic with 6 months of hoarseness and poor voice projection without improvement from speech therapy or medical anti-reflux medication. Upon examination he was found to have multiple polypoid lesions emanating from bilateral false vocal folds, left true vocal fold, and the anterior commissure. Biopsy and potassium titanyl phosphate (KTP) laser ablation with bevacizumab injection provided treatment and confirmed the clinical suspicion of squamous cell papilloma...
March 28, 2018: Head and Neck Pathology
https://www.readbyqxmd.com/read/29593217/structural-characterization-of-a-highly-potent-v3-glycan-broadly-neutralizing-antibody-bound-to-natively-glycosylated-hiv-1-envelope
#4
Christopher O Barnes, Harry B Gristick, Natalia T Freund, Amelia Escolano, Artem Y Lyubimov, Harald Hartweger, Anthony P West, Aina E Cohen, Michel C Nussenzweig, Pamela J Bjorkman
Broadly neutralizing antibodies (bNAbs) isolated from HIV-1-infected individuals inform HIV-1 vaccine design efforts. Developing bNAbs with increased efficacy requires understanding how antibodies interact with the native oligomannose and complex-type N-glycan shield that hides most protein epitopes on HIV-1 envelope (Env). Here we present crystal structures, including a 3.8-Å X-ray free electron laser dataset, of natively glycosylated Env trimers complexed with BG18, the most potent V3/N332gp120 glycan-targeting bNAb reported to date...
March 28, 2018: Nature Communications
https://www.readbyqxmd.com/read/29556225/differential-immunoreactivity-to-bovine-convalescent-serum-between-mycoplasma-bovis-biofilms-and-planktonic-cells-revealed-by-comparative-immunoproteomic-analysis
#5
Shengli Chen, Huafang Hao, Ping Zhao, Wenheng Ji, Mingxia Li, Yongsheng Liu, Yuefeng Chu
Mycoplasma bovis is a major bovine pathogen that causes considerable economic losses in the cattle industry worldwide. Moreover, M. bovis biofilm can persist in the environment and its host. To date, M. bovis biofilm antigens recognized by bovine convalescent sera and their comparison with planktonic cells have not yet been explored. This study utilized an immunoproteomic approach using two-dimensional electrophoresis, immunoblotting using convalescent bovine serum, and subsequent matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF MS) to identify the immunoreactive proteins expressed in biofilm- and planktonic-grown M...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29508543/photothermally-controlled-mhc-class-i-restricted-cd8-t-cell-responses-elicited-by-hyaluronic-acid-decorated-gold-nanoparticles-as-a-vaccine-for-cancer-immunotherapy
#6
Fengqiang Cao, Mengmeng Yan, Yijia Liu, Lanxia Liu, Guilei Ma
Cancer vaccines aim to induce a strong major histocompatibility complex class I (MHC-I)-restricted CD8+ cytotoxic T-cell response, which is an important prerequisite for successful cancer immunotherapy. Herein, a hyaluronic acid (HA) and antigen (ovalbumin, OVA)-decorated gold nanoparticle (AuNPs)-based (HA-OVA-AuNPs) vaccine is developed for photothermally controlled cytosolic antigen delivery using near-infrared (NIR) irradiation and is found to induce antigen-specific CD8+ T-cell responses. Chemical binding of thiolated HA and OVA to AuNPs facilitates antigen uptake of dendritic cells via receptor-mediated endocytosis...
March 6, 2018: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/29492465/current-and-future-management-of-recurrent-respiratory-papillomatosis
#7
REVIEW
Ryan Ivancic, Hassan Iqbal, Brad deSilva, Quintin Pan, Laura Matrka
Objectives: Recurrent respiratory papillomatosis (RRP) is a chronic disease of the respiratory tract that occurs in both children and adults. It is caused by the human papillomavirus (HPV), in particular low-risk HPV6 and HPV11, and aggressiveness varies among patients. RRP remains a chronic disease that is difficult to manage. This review provides perspectives on current and future management of RRP. Results: The current standard of care is surgical excision, with adjuvant therapies as needed...
February 2018: Laryngoscope Investigative Otolaryngology
https://www.readbyqxmd.com/read/29491457/towards-in-cellulo-virus-crystallography
#8
Helen M E Duyvesteyn, Helen M Ginn, Maija K Pietilä, Armin Wagner, Johan Hattne, Jonathan M Grimes, Elina Hirvonen, Gwyndaf Evans, Marie-Laure Parsy, Nicholas K Sauter, Aaron S Brewster, Juha T Huiskonen, David I Stuart, Geoff Sutton, Dennis H Bamford
Viruses are a significant threat to both human health and the economy, and there is an urgent need for novel anti-viral drugs and vaccines. High-resolution viral structures inform our understanding of the virosphere, and inspire novel therapies. Here we present a method of obtaining such structural information that avoids potentially disruptive handling, by collecting diffraction data from intact infected cells. We identify a suitable combination of cell type and virus to accumulate particles in the cells, establish a suitable time point where most cells contain virus condensates and use electron microscopy to demonstrate that these are ordered crystalline arrays of empty capsids...
February 28, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29447484/cationic-dda-tdb-liposome-as-a-mucosal-vaccine-adjuvant-for-uptake-by-dendritic-cells-in-vitro-induces-potent-humoural-immunity
#9
Wenjing Qu, Na Li, Rui Yu, Wenbao Zuo, Tingting Fu, Wenling Fei, Yanhui Hou, Yanhua Liu, Jianhong Yang
The cationic dimethyldioctadecylammonium/trehalose 6,6,9-dibehenate (DDA/TDB) liposome is as a strong adjuvant system for vaccines, with remarkable immunostimulatory activity. The mucosal administration of vaccines is a potential strategy for inducing earlier and stronger mucosal immune responses to infectious diseases. In this study, we assessed whether the intranasal administration of cationic DDA/TDB liposomes combined with influenza antigen A (H3N2) can be used as a highly efficacious vaccine to induce mucosal and systemic antibody responses...
February 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29436537/photosensitizer-induced-self-assembly-of-antigens-as-nanovaccines-for-cancer-immunotherapy
#10
F Q Cao, M M Yan, Y J Liu, L X Liu, L Lu, H Wang, Ch Zhang, H F Sun, D L Kong, G L Ma
Herein, the photosensitizer indocyanine green (ICG) is used to induce the self-assembly of antigens to form nanovaccines. Under near-infrared (NIR) laser irradiation, reactive oxygen species can be generated by nanovaccines to disrupt the membranes of endo/lysosomes, which helps to release antigens into the cytosol efficiently, thereby enhancing antigen cross-presentation and anti-cancer immunity. To the best of our knowledge, this study represents the first example of ICG as a biocompatible adjuvant to improve cancer vaccine efficacy...
February 27, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29420802/maldi-tof-ms-analysis-of-bovine-and-zoonotic-trichophyton-verrucosum-isolates-reveals-a-distinct-peak-and-cluster-formation-of-a-subgroup-with-trichophyton-benhamiae
#11
Theresa Bartosch, Tilo Heydel, Silke Uhrlaß, Pietro Nenoff, Hendrik Müller, Christoph Georg Baums, Wieland Schrödl
The zoophilic dermatophyte Trichophyton verrucosum is the most important causative agent of bovine dermatophytosis. Additionally, it causes profound and poorly healing skin infections in humans indicating the high zoonotic potential. The objective of this study was to establish differentiation of T. verrucosum from other dermatophytes by mass spectrometry and to identify distinct features of the mass spectra. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was successful for identification of this pathogen only after extension of the database of the manufacturer with spectra from T...
October 9, 2017: Medical Mycology: Official Publication of the International Society for Human and Animal Mycology
https://www.readbyqxmd.com/read/29416336/maturation-of-dendritic-cells-in-vitro-and-immunological-enhancement-of-mice-in-vivo-by-pachyman-and-or-ova-encapsulated-poly-d-l-lactic-acid-nanospheres
#12
Sisi Zheng, Tao Qin, Yu Lu, Yifan Huang, Li Luo, Zhenguang Liu, Ruonan Bo, Yuanliang Hu, Jiaguo Liu, Deyun Wang
Background: Poly lactide (PLA) was proved in the last years to be good for use in sustained drug delivery and as carriers for vaccine antigens. In our previous research, pachyman (PHY)-encapsulated PLA (PHYP) nanospheres were synthesized and their function of controlling drug release was demonstrated. Purpose: In order to modify the fast drug-release rate of PHY when inoculated alone, the maturation of bone marrow dendritic cells (BMDCs) in vitro and their immunological enhancement in vivo were explored using PHYP nanospheres...
2018: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29382195/vx3-functionalized-alumina-nanoparticles-assisted-enrichment-of-ubiquitinated-proteins-from-cancer-cells-for-enhanced-cancer-immunotherapy
#13
Jinjin Zhao, Ning Pan, Fang Huang, Mohanad Aldarouish, Zhifa Wen, Rong Gao, Yuye Zhang, Hong-Ming Hu, Yanfei Shen, Li-Xin Wang
A simple and effective strategy was developed to enrich ubiquitinated proteins (UPs) from cancer cell lysate using the α-Al2 O3 nanoparticles covalently linked with ubiquitin binding protein (Vx3) (denoted as α-Al2 O3 -Vx3) via a chemical linker. The functionalized α-Al2 O3 -Vx3 showed long-term stability and high efficiency for the enrichment of UPs from cancer cell lysates. Flow cytometry analysis results indicated dendritic cells (DCs) could more effectively phagocytize the covalently linked α-Al2 O3 -Vx3-UPs than the physical mixture of α-Al2 O3 and Vx3-UPs (α-Al2 O3 /Vx3-UPs)...
March 21, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29360648/artificial-intelligence-estimates-the-impact-of-human-papillomavirus-types-in-influencing-the-risk-of-cervical-dysplasia-recurrence-progress-toward-a-more-personalized-approach
#14
Giorgio Bogani, Antonino Ditto, Fabio Martinelli, Mauro Signorelli, Valentina Chiappa, Umberto Leone Roberti Maggiore, Francesca Taverna, Claudia Lombardo, Chiara Borghi, Cono Scaffa, Domenica Lorusso, Francesco Raspagliesi
The objective of this study was to determine whether the pretreatment human papillomavirus (HPV) genotype might predict the risk of cervical dysplasia persistence/recurrence. Retrospective analysis of prospectively collected data of consecutive 5104 women who underwent the HPV-DNA test were matched with retrospective data of women undergoing either follow-up or medical/surgical treatment(s) for genital HPV-related infection(s). Artificial neuronal network (ANN) analysis was used in order to weight the importance of different HPV genotypes in predicting cervical dysplasia persistence/recurrence...
January 22, 2018: European Journal of Cancer Prevention
https://www.readbyqxmd.com/read/29344312/an-endogenous-vaccine-based-on-fluorophores-and-multivalent-immunoadjuvants-regulates-tumor-micro-environment-for-synergistic-photothermal-and-immunotherapy
#15
Ling Li, Suleixin Yang, Linjiang Song, Yan Zeng, Tao He, Ning Wang, Chuan Yu, Tao Yin, Li Liu, Xiawei Wei, Qinjie Wu, Yuquan Wei, Li Yang, Changyang Gong
Recently, near-infrared (NIR) light-based photothermal therapy (PTT) has been widely applied in cancer treatment. However, in most cases, the tissue penetration depth of NIR light is not sufficient and thus photothermal therapy is unable to completely eradicate deep, seated tumors inevitably leading to recurrence of the tumor. Due to this significant limitation of NIR, improved therapeutic strategies are urgently needed. Methods: We developed an endogenous vaccine based on a novel nanoparticle platform for combinatorial photothermal ablation and immunotherapy...
2018: Theranostics
https://www.readbyqxmd.com/read/29337913/proteomic-analysis-and-identification-of-possible-allergenic-proteins-in-mature-pollen-of-populus-tomentosa
#16
Liuqiang Wang, Xiaoling Zhang, Jin Zhang, Wei Fan, Mengzhu Lu, Jianjun Hu
Pollen grains from Populus tomentosa , a widely cultivated tree in northern area of China, are considered to be an important aeroallergen causing severe allergic diseases. To gain insight into their allergenic components, mature Populus tomentosa pollen proteins were analyzed by two-dimensional gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF/TOF MS). A total of 412 spots from mature pollen were resolved on pH 4-7 immobilized pH gradient (IPG) strips and 159 distinct proteins were identified from 242 spots analyzed...
January 16, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29334981/production-of-highly-and-broad-range-specific-monoclonal-antibodies-against-hemagglutinin-of-h5-subtype-avian-influenza-viruses-and-their-differentiation-by-mass-spectrometry
#17
Violetta Sączyńska, Anna Bierczyńska-Krzysik, Violetta Cecuda-Adamczewska, Piotr Baran, Anna Porębska, Katarzyna Florys, Marcin Zieliński, Grażyna Płucienniczak
BACKGROUND: The highly pathogenic avian influenza viruses of the H5 subtype, such as the H5N1 viral strains or the novel H5N8 and H5N2 reassortants, are of both veterinary and public health concern worldwide. To combat these viruses, monoclonal antibodies (mAbs) against H5 hemagglutinin (HA) play a significant role. These mAbs are effective diagnostic and therapeutic agents and powerful tools in vaccine development and basic scientific research. The aim of this study was to obtain diagnostically valuable mAbs with broad strain specificity against H5-subtype AIVs...
January 15, 2018: Virology Journal
https://www.readbyqxmd.com/read/29321677/recognition-by-host-nuclear-transport-proteins-drives-disorder-to-order-transition-in-hendra-virus-v
#18
Sarah C Atkinson, Michelle D Audsley, Kim G Lieu, Glenn A Marsh, David R Thomas, Steven M Heaton, Jason J Paxman, Kylie M Wagstaff, Ashley M Buckle, Gregory W Moseley, David A Jans, Natalie A Borg
Hendra virus (HeV) is a paramyxovirus that causes lethal disease in humans, for which no vaccine or antiviral agent is available. HeV V protein is central to pathogenesis through its ability to interact with cytoplasmic host proteins, playing key antiviral roles. Here we use immunoprecipitation, siRNA knockdown and confocal laser scanning microscopy to show that HeV V shuttles to and from the nucleus through specific host nuclear transporters. Spectroscopic and small angle X-ray scattering studies reveal HeV V undergoes a disorder-to-order transition upon binding to either importin α/β1 or exportin-1/Ran-GTP, dependent on the V N-terminus...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29259271/clearance-of-a-persistent-picornavirus-infection-is-associated-with-enhanced-pro-apoptotic-and-cellular-immune-responses
#19
Carolina Stenfeldt, Michael Eschbaumer, George R Smoliga, Luis L Rodriguez, James Zhu, Jonathan Arzt
Long-term persistent viral infections cause substantial morbidity and associated economic losses in human and veterinary contexts. Yet, the mechanisms associated with establishment of persistent infections are poorly elucidated. We investigated immunomodulatory mechanisms associated with clearance versus persistence of foot-and-mouth disease virus (FMDV) in micro-dissected compartments of the bovine nasopharynx by microarray. The use of laser-capture microdissection allowed elucidation of differential gene regulation within distinct anatomic compartments critical to FMDV infection...
December 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29220398/a-minimally-invasive-optical-trapping-system-to-understand-cellular-interactions-at-onset-of-an-immune-response
#20
David G Glass, Niall McAlinden, Owain R Millington, Amanda J Wright
T-cells and antigen presenting cells are an essential part of the adaptive immune response system and how they interact is crucial in how the body effectively fights infection or responds to vaccines. Much of the experimental work studying interaction forces between cells has looked at the average properties of bulk samples of cells or applied microscopy to image the dynamic contact between these cells. In this paper we present a novel optical trapping technique for interrogating the force of this interaction and measuring relative interaction forces at the single-cell level...
2017: PloS One
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