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https://www.readbyqxmd.com/read/29234944/investigating-the-mincing-method-for-isolation-of-adipose-derived-stem-cells-from-pregnant-women-fat
#1
Yuan-Sheng Li, Pao-Jen Chen, Li-Wei Wu, Pei-Wen Chou, Li-Yi Sun, Tzyy-Wen Chiou
The success of stem cell application in regenerative medicine, usually require a stable source of stem or progenitor cells. Fat tissue represents a good source of stem cells because it is rich in stem cells and there are fewer ethical issues related to the use of such stem cells, unlike embryonic stem cells. Therefore, there has been increased interest in adipose-derived stem cells (ADSCs) for tissue engineering applications. Here, we aim to provide an easy processing method for isolating adult stem cells from human adipose tissue harvested from the subcutaneous fat of the abdominal wall during gynecologic surgery...
December 12, 2017: Cytotechnology
https://www.readbyqxmd.com/read/29232530/pediatric-endoscopic-pilonidal-sinus-treatment-a-revolutionary-technique-to-adopt-in-children-with-pilonidal-sinus-fistulas-our-preliminary-experience
#2
Ciro Esposito, Serena Izzo, Francesco Turrà, Mariapina Cerulo, Giovanni Severino, Alessandro Settimi, Marta Iannazzone, Lorenzo Masieri, Giuseppe Cortese, Maria Escolino
AIM: This study aimed to report our preliminary experience with pediatric endoscopic pilonidal sinus treatment (PEPSiT). PATIENTS AND METHODS: We retrospectively reviewed the reports of 15 patients, 6 girls and 9 boys, with an average age of 16 years (range 13-18) with noninfected pilonidal sinus disease who underwent PEPSiT in our institution over an 18-month period. Four cases were redo-procedures, for recurrence of disease after open excision repair. Surgical outcomes of sinus healing, recurrence of disease, postoperative pain, hospital stay, analgesic requirements, and patient satisfaction levels were evaluated and a comparison analysis with classic open repair was performed...
December 12, 2017: Journal of Laparoendoscopic & Advanced Surgical Techniques. Part A
https://www.readbyqxmd.com/read/29227514/management-and-outcomes-of-retained-lenticules-and-lenticule-fragments-removal-after-failed-primary-smile-a-case-series
#3
Sri Ganesh, Sheetal Brar, Apostolos Lazaridis
PURPOSE: To describe the management of and report the outcomes following the removal of retained lenticules or lenticule fragments after a complicated small incision lenticule extraction (SMILE). METHODS: Three patients were referred for consultation due to intraoperative complications during SMILE. In case 1, the lenticule was torn during extraction and a central fragment was retained in the pocket. In case 2, the inferior part of the lenticule remained attached at the anterior plane and its detached, superior part was dislocated and folded at the inferior part of the pocket...
December 1, 2017: Journal of Refractive Surgery
https://www.readbyqxmd.com/read/29225197/percutaneous-transgastrostomic-interventional-radiology-operated-endoscopy-facilitates-foreign-body-removal-using-rigid-endobronchial-forceps
#4
Mamdouh Khayat, Jawad S Hussain, Jeffrey Forris Beecham Chick, Anthony N Hage, Ravi N Srinivasa
Retained foreign bodies within the stomach and proximal small bowel may be problematic in patients with prior cerebrovascular injury or head, neck and esophageal malignancy, given the increased vulnerability of this patient population to complications from aspiration and increased difficulty of esophagogastroduodenoscopy in cases of tumoral obstruction. This article presents an alternative method for foreign body retrieval through an existing gastrostomy tract, which offers the benefits of fast procedure times, reduction in radiation dose and fluoroscopy time, and allows for safer retrieval of foreign bodies by using direct visualization...
December 8, 2017: Diagnostic and Interventional Radiology: Official Journal of the Turkish Society of Radiology
https://www.readbyqxmd.com/read/29216530/brain-angiometastasis-from-a-non-seminomatous-germ-cell-tumor-a-case-report
#5
Alejandro Monroy-Sosa, Gervith Reyes-Soto, Bernardo Cacho-Díaz, Martín Granados-García, Allan Hernández Estrada, Ana María Cano-Valdez, Ángel Herrera-Gómez
INTRODUCTION: Brain metastasis from non-seminomatous germ cell tumors (NSGCT) is rare. Herein, we describe the second reported case of brain metastasis from a NSGCT with high-flow arteriovenous (AV) shunts, and propose a novel surgical treatment plan. CLINICAL CASE: The patient was a 34-year-old male who presented with hemiparesis and hemianesthesia. Magnetic resonance angiography revealed three vascular lesions with afferent vessels and efferent vessels. Angiography displayed two high-flow AV shunts...
December 2, 2017: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/29214060/interstitial-lung-disease-the-diagnostic-role-of-bronchoscopy
#6
REVIEW
Jad Kebbe, Tony Abdo
Interstitial lung diseases (ILDs) form one of the most fascinating fields in pulmonary medicine. They also pose one of the greatest challenges for accurate diagnosis and proper treatment. Even within the recommended and warranted multidisciplinary approach, differentiating between one disease and another may lead to frustration, especially when proper lung tissue is not available for adequate pathological review. A surgical lung biopsy (SLB) might render enough tissue for histopathology, but this could come at the expense of high morbidity and even mortality, as in the case of usual interstitial pneumonia (UIP)...
September 2017: Journal of Thoracic Disease
https://www.readbyqxmd.com/read/29208822/b-hex-pupil-expander-pupil-expansion-redefined
#7
Suven Bhattacharjee
The B-HEX® Pupil Expander (Med Invent Devices) is a disposable 6.5 mm flexible hexagonal device with notches at corners and flanges at sides. Alternate flanges with positioning holes are tucked under the iris to engage the notches to the margin of the pupil to provide a 5.5 mm expanded pupil. Unlike devices with scrolls or pockets which require an injector to avoid snagging the incision, the preloaded B-HEX is inserted and removed through a 1 mm or larger incision using a manipulator or 23-gauge micro-forceps...
December 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29205394/vessel-sealer-and-divider-instrument-temperature-during-laparoscopic-ovariectomy-in-horses
#8
Jeremiah T Easley, Kirk C McGilvray, Dean A Hendrickson, Jason Bruemmer, Eileen S Hackett
OBJECTIVE: To determine the temperature of a vessel sealer and divider device during unilateral paralumbar laparoscopic ovariectomy in standing, sedated mares. STUDY DESIGN: Prospective study. ANIMALS: Fifteen healthy research mares. METHODS: Healthy mares with normal ovarian palpation and ultrasonographic appearance were enrolled. Horses were restrained in standing stocks and sedated. A right or left paralumbar ovariectomy was performed with a laparoscopic portal and 2 instrument portals...
December 3, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29200087/rapid-explantation-of-rapid-deployment-sutureless-valve-in-case-of-acute-endocarditis-how-to-remove-safely-sutureless-perceval-s-prostheses
#9
Lorenzo Di Bacco, Steffen Pfeiffer, Theodor J M Fischlein, Giuseppe Santarpino
We report a case of infective endocarditis on a Perceval S aortic valve bioprosthesis, and we describe a feasible and useful technique that can help remove the infected valve. These maneuvers consist of an "x-movement" reached with the aid of anatomical forceps. The risk of infective endocarditis in patients with prosthetic valves is a well-known phenomena in cardiac surgery, and it can represent a life-threatening event in many cases. According to guidelines, an emergency surgical intervention is required in case of high risk of embolization, vegetation superior than 1 cm or acute cardiac failure secondary to the acute valve dysfunction...
December 1, 2017: Innovations: Technology and Techniques in Cardiothoracic and Vascular Surgery
https://www.readbyqxmd.com/read/29199444/steering-sheath-for-2-nostril-transnasal-office-laryngoscopy
#10
Henry T Hoffman, Helen Stegall, Troy Wingler, Andrew Blitzer
OBJECTIVE: The aim was to study the feasibility of performing office-based laryngeal procedures employing a flexible hollow steerable sheath placed contralateral to the nostril through which a standard flexible video endoscope is placed. METHODS: The study design included simulation of transnasal endoscopic laryngeal procedures evaluating the use of a flexible steering sheath in laboratory and clinic settings. Transnasal laryngeal procedures were performed in an otolaryngology office setting employing an airway-management-trainer mannequin and then repeated in a human cadaver lab with standard transnasal flexible video laryngoscopy...
December 1, 2017: Annals of Otology, Rhinology, and Laryngology
https://www.readbyqxmd.com/read/29197159/intra-ocular-diathermy-forceps
#11
Koen A van Overdam, Emine Kilic, Robert M Verdijk, Sonia Manning
PURPOSE: The purpose of this study was to develop intra-ocular diathermy forceps and test them on perfused porcine cadaver eyes. METHODS: We designed two types of 23-gauge intra-ocular bipolar diathermy forceps by modifying commercially available membrane peeling forceps. In the first type, the emitting electrode is connected to one-half of the core and the return electrode to the other half, with one jaw of the forceps attached to each half. In the second type, the emitting electrode is attached to the core and both jaws of the forceps, and the return electrode to the surrounding tube...
December 2, 2017: Acta Ophthalmologica
https://www.readbyqxmd.com/read/29183203/assessing-the-diagnostic-yield-of-controllable-biopsy-forceps-for-biliary-strictures
#12
Tadahisa Inoue, Rena Kitano, Yuji Kobayashi, Norimitsu Ishii, Kazumasa Sakamoto, Tomohiko Ohashi, Yukiomi Nakade, Yoshio Sumida, Kiyoaki Ito, Haruhisa Nakao, Masashi Yoneda
OBJECTIVE: Biliary forceps biopsies are essential for differentially diagnosing biliary strictures and evaluating the preoperative superficial intraductal spread of bile duct cancers; however, these biopsies are technically demanding and time consuming. Using controllable biopsy-forceps (C-BF), which enable the tip's angle to be adjusted by up to 90°, may facilitate the procedure and improve the diagnostic yield for biliary biopsies. This study aimed to examine the efficacy of C-BF associated with the diagnosis of biliary strictures...
November 28, 2017: Scandinavian Journal of Gastroenterology
https://www.readbyqxmd.com/read/29163330/white-matter-changes-in-hiv-women-with-a-history-of-cocaine-dependence
#13
Kathryn-Mary Wakim, Ciara J Molloy, Ryan P Bell, Lars A Ross, John J Foxe
Cocaine use is associated with the transmission of human immunodeficiency (HIV) virus through risky sexual behavior. In HIV+ individuals, cocaine use is linked with poor health outcomes, including HIV-medication non-adherence and faster disease progression. Both HIV and cocaine dependence are associated with reduced integrity of cerebral white matter (WM), but the effects of HIV during cocaine abstinence have not yet been explored. We used diffusion tensor imaging (DTI) to understand the effect of combined HIV+ serostatus and former cocaine dependence on cerebral WM integrity...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/29162988/suture-assisted-punctoplasty
#14
Amun Sachdev, Suresh R Sagili
Purpose: To describe a surgical technique in which a suture, instead of forceps, is used to improve access for the introduction of scissors and more easily achieve an appropriately-sized punctoplasty. Materials and Methods: In this technique, a new modification of the 2-snip punctoplasty, a 6-0 polyglactin 910 suture is passed through the posterior wall of the punctum to apply traction. A video of the technique is provided. Results: This technique improves the surgical field of view and eases access for introduction of Vannas scissors into the punctum to perform the punctoplasty...
2017: Digital Journal of Ophthalmology: DJO
https://www.readbyqxmd.com/read/29162068/new-steps-of-robot-assisted-radical-prostatectomy-using-the-extraperitoneal-approach-a-propensity-score-matched-comparison-between-extraperitoneal-and-transperitoneal-approach-in-japanese-patients
#15
Satoshi Kurokawa, Yukihiro Umemoto, Kentaro Mizuno, Atsushi Okada, Akihiro Nakane, Hidenori Nishio, Shuzo Hamamoto, Ryosuke Ando, Noriyasu Kawai, Keiichi Tozawa, Yutaro Hayashi, Takahiro Yasui
BACKGROUND: Robot-assisted radical prostatectomy (RARP) is commonly performed using the transperitoneal (TP) approach with six trocars over an 8-cm distance in the steep Trendelenburg position. In this study, we investigated the feasibility and the benefit of using the extraperitoneal (EP) approach with six trocars over a 4-cm distance in a flat or 5° Trendelenburg position. We also introduced four new steps to the surgical procedure and compared the surgical results and complications between the EP and TP approach using propensity score matching...
November 21, 2017: BMC Urology
https://www.readbyqxmd.com/read/29158099/a-novel-dissection-method-using-a-flexible-neuroendoscope-for-resection-of-tumors-around-the-aqueduct-of-sylvius-a-case-report
#16
Takayuki Ishikawa, Kazuhito Takeuchi, Nobuhiro Tsukamoto, Teppei Kawabata, Toshihiko Wakabayashi
Flexible endoscopes have both a wide range of movement and a wide field of view and are thus widely used for endoscopic third ventriculostomy and biopsy. However, tumor resection using flexible endoscopes has scarcely been reported, likely owing to their single port, which has a diameter of 1.2-2.0 mm. Furthermore, flexible endoscopes do not allow some of the basic neurosurgical manipulations, especially sharp dissection with counter tension. Additionally, the position of the forceps and coagulators is fixed within the endoscope...
November 17, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29155777/transurethral-instillation-procedure-in-adult-male-mouse
#17
Sanghee Lee, Alonso Carrasco, Randall B Meacham, Anna P Malykhina
Transurethral instillation can be used to deliver different solutions with active ingredients (e.g., drugs, chemicals, bacteria, and viruses) locally into the urinary bladder to either induce animal models of bladder pathologies or evaluate the effectiveness of intravesical treatments. Most rodent models of lower urinary tract (LUT) pathologies are induced in female mice due to ease of intravesical instillation of the substances via the female urethra. However, due to anatomical differences between the female and male LUT, transurethral instillation in a male mouse has been deemed a very challenging procedure, and it has not been previously described...
November 2, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155140/pregnancy-and-parturition-negatively-impact-vaginal-angle-and-alters-expression-of-vaginal-mmp-9
#18
Sallie Oliphant, Timothy Canavan, Stacy Palcsey, Leslie Meyn, Pamela Moalli
BACKGROUND: Parity is the greatest risk factor for the development of pelvic organ prolapse. The normally supported vagina is pulled up and back over the levator ani. Loss of vaginal angulation has been associated with prolapse and may represent injury to the vaginal supportive tissues. OBJECTIVE: We propose that 1) pregnancy and delivery impact support to the vagina leading to loss of vaginal angle; 2) vaginal angulation is restored postpartum; and 3) uncomplicated vaginal delivery (VD) is associated with accelerated remodeling of the vaginal fibrillar matrix...
November 16, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29151936/multiple-guided-technologies-based-on-radial-probe-endobronchial-ultrasound-for-the-diagnosis-of-solitary-peripheral-pulmonary-lesions-a-single-center-study
#19
Huang Haidong, Ning Yunye, Zhang Wei, Paul Zarogoulidis, Wolfgang Hohenforst-Schmidt, Yan-Gao Man, Yang Yuguang, Dong Yuchao, Bai Chong
Objective To evaluate the value of multiple guided technologies based on radial probe endobronchial ultrasound (R-EBUS) in the application of the diagnosis of solitary pulmonary peripheral lesions (PPLs). Methods Analysis of diagnostic yield in 4 groups patients with different combined multiple guided technologies based on R-EBUS, 94 patients with 94 solitary PPLs from Mar, 2013 to Nov, 2014 in Changhai Hospital. Results The overall diagnostic yield was 75% (70/94), the diagnostic yield of Group A (R-EBUS) was 62%(16/26), Group B (R-EBUS with guided sheath, EBUS-GS) was 76% (34/45), Group C (EBUS-GS with fluoroscopy) was 82% (9/11), Group D (virtual bronchoscopic navigation guided EBUS-GS with fluoroscopy) was 92% (11/12)...
2017: Journal of Cancer
https://www.readbyqxmd.com/read/29150230/cesarean-birth-what-s-in-a-name
#20
L Ni, A Elsaharty, I McConachie
BACKGROUND: Cesarean birth is known as both cesarean section (CS) or cesarean delivery (CD). The International Journal of Obstetric Anesthesia (IJOA) is the leading obstetric anesthesia journal, and a barometer of attitudes within the profession. The journal recently published the hundredth issue, spanning 25 years (to December 2016). It is an opportune time to examine the evolution of surgical birth terminology (CS versus CD) during that period. METHODS: We examined 1583 articles in IJOA, subdividing them into editorials, papers, review articles, debates and case reports...
September 28, 2017: International Journal of Obstetric Anesthesia
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