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https://www.readbyqxmd.com/read/29028629/a-dual-energy-micro-ct-methodology-for-visualization-and-quantification-of-biofilm-formation-and-dentin-demineralization
#1
Paula Maciel Pires, Thais Pires Dos Santos, Andrea Fonseca-Gonçalves, Matheus Melo Pithon, Ricardo Tadeu Lopes, Aline de Almeida Neves
OBJECTIVE: The aim of this study was to induce artificial caries in human sound dentin by means of a microcosm model using human saliva as source of bacteria and to apply a novel dual-energy micro-CT technique to quantify biofilm formation and evaluate its demineralization potential. DESIGN: Eight sound third molars had the occlusal enamel removed by cutting with a diamond disk and five cylindrical cavities (±2mm diameter; ±1.5mm depth) were prepared over the dentin surface in each specimen (n=40 cavities)...
October 7, 2017: Archives of Oral Biology
https://www.readbyqxmd.com/read/29022762/polymorphism-rs11656696-in-gas7-is-not-associated-with-primary-open-angle-glaucoma-in-a-saudi-cohort
#2
Altaf A Kondkar, Taif A Azad, Faisal A Almobarak, Hatem Kalantan, Tahira Sultan, Saleh A Al-Obeidan, Khaled K Abu-Amero
AIMS: To conduct a case-control study to investigate the association between the polymorphism rs11656696 located in the growth arrest-specific 7 gene on human chromosome 17p13.1 and primary open angle glaucoma (POAG). METHODS: The polymorphism rs11656696 was genotyped using the TaqMan(®) assay in 187 subjects comprising 92 unrelated POAG cases and 95 controls of Saudi Arabian origin. RESULTS: Association analysis between cases and controls revealed no significant genotype distribution under additive (p = 0...
October 12, 2017: Genetic Testing and Molecular Biomarkers
https://www.readbyqxmd.com/read/28964545/diagnostic-imaging-of-discospondylitis
#3
REVIEW
Catherine M Ruoff, Sharon C Kerwin, Amanda R Taylor
Discospondylitis can affect dogs of any age and breed and may be seen in cats. Although radiography remains the gold standard, advanced imaging, such as CT and MRI, has benefits and likely allows earlier diagnosis and identification of concurrent disease. Because discospondylitis may affect multiple disk spaces, imaging of the entire spine should be considered. There is a lengthening list of causative etiologic agents, and successful treatment hinges on correct identification. Image-guided biopsy should be considered in addition to blood and urine cultures and Brucella canis screening and as an alternative to surgical biopsy in some cases...
September 27, 2017: Veterinary Clinics of North America. Small Animal Practice
https://www.readbyqxmd.com/read/28954541/standardized-reactors-for-the-study-of-medical-biofilms-a-review-of-the-principles-and-latest-modifications
#4
Inês B Gomes, Ana Meireles, Ana L Gonçalves, Darla M Goeres, Jelmer Sjollema, Lúcia C Simões, Manuel Simões
Biofilms can cause severe problems to human health due to the high tolerance to antimicrobials; consequently, biofilm science and technology constitutes an important research field. Growing a relevant biofilm in the laboratory provides insights into the basic understanding of the biofilm life cycle including responses to antibiotic therapies. Therefore, the selection of an appropriate biofilm reactor is a critical decision, necessary to obtain reproducible and reliable in vitro results. A reactor should be chosen based upon the study goals and a balance between the pros and cons associated with its use and operational conditions that are as similar as possible to the clinical setting...
September 27, 2017: Critical Reviews in Biotechnology
https://www.readbyqxmd.com/read/28948122/a-case-of-persistent-low-back-pain-in-a-young-female-caused-by-a-trauma-induced-schmorl-s-node-in-the-lumbar-spine-five-vertebra
#5
Kurt M Mohty, Divneet Mandair, Brent Munroe, Deborah Baldemor
Physicians are often faced with managing difficult conditions such as chronic lower back pain. Intervertebral disk herniation typically occurs horizontally, leading to impingement of the spinal cord which can potentially cause radicular symptoms or other spinal cord pathologies; however, disk herniations can also occur vertically and extend through the endplate of an adjacent cranial or caudal vertebra: a phenomenon known as a Schmorl's node. Although Schmorl's nodes can be seen in many asymptomatic individuals, they can be a cause of degenerative disk disease and low back pain...
July 21, 2017: Curēus
https://www.readbyqxmd.com/read/28945642/clinical-and-radiologic-comparison-of-minimally-invasive-surgery-with-traditional-open-transforaminal-lumbar-interbody-fusion-a-review-of-452-patients-from-a-single-center
#6
Julian P Price, John M Dawson, James D Schwender, Kurt P Schellhas
STUDY DESIGN: Retrospective analysis of prospective data for parallel, consecutive series of patients (Level III). OBJECTIVE: Compare clinical results and radiographic outcomes of minimally invasive surgery (MIS) versus open techniques for transforaminal lumbar interbody fusion (TLIF). SUMMARY OF BACKGROUND DATA: Minimally invasive techniques allow transforaminal exposure with decreased soft tissue disruption, but the question remains whether surgical and functional outcomes are equivalent to open techniques...
September 22, 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28937464/comparison-of-clinical-outcomes-after-anterior-cervical-discectomy-and-fusion-versus-cervical-total-disk-replacement-in-patients-with-modic-2-changes-on-mri
#7
Jia Li, Di Zhang, Wenyuan Ding, Yingze Zhang, Yong Shen
STUDY DESIGN: A retrospective investigation. OBJECTIVE: The aim of this research is to compare the clinical and radiologic outcomes of patients with Modic-2 changes who underwent anterior cervical discectomy and fusion (ACDF) or cervical total disk replacement (TDR) in single level. SUMMARY OF BACKGROUND DATA: There were few studies focused on the clinical and radiologic outcomes of patients with Modic-2 changes who underwent ACDF or cervical TDR in single level...
October 2017: Clinical Spine Surgery
https://www.readbyqxmd.com/read/28924335/optical-coherence-tomography-angiography-to-better-understand-glaucoma
#8
EDITORIAL
Gábor Holló
The term optical coherence tomography angiography (OCTA) comprises different OCT-based technologies which all allow noninvasive assessment of retinal perfusion, based on moving red blood cells. The main areas where OCTA is currently used are investigation of perfusion and vascular structure of the macular retina (e.g., in macular degenerations and diabetic macular diseases) and the disk and peripapillary retina (in glaucoma and other optic disk diseases). The current editorial provides a brief overview on the potential of OCTA and its use to measure perfusion in the peripapillary retina in glaucoma...
May 2017: Journal of Current Glaucoma Practice
https://www.readbyqxmd.com/read/28919687/presence-and-characterization-of-escherichia-coli-virulence-genes-isolated-from-diseased-pigs-in-the-central-region-of-argentina
#9
Fernando A Bessone, Gabriela Bessone, Sebastián Marini, María B Conde, Fabrisio E Alustiza, Gustavo Zielinski
BACKGROUND: The main pathogen of neonatal and post weaning diarrhea and edema disease (ED) is Escherichia coli and pathotypes involved are enterotoxigenic, enteropathogenic, and shiga toxigenic (ETEC, EPEC, and STEC, respectively). Those diseases cause economic loss in pig production. AIM: The aim of this work was to evaluate the presence of strains expressing virulence markers genes and the antibiotic susceptibility profiles of E. coli from clinical cases of post weaning diarrhea and ED in farms in the central area of Argentina...
August 2017: Veterinary World
https://www.readbyqxmd.com/read/28916321/analysis-of-resistance-genes-in-pan-resistant-myroides-odoratimimus-clinical-strain-pr63039-using-whole-genome-sequencing
#10
De-Song Ming, Qing-Qing Chen, Xiao-Tin Chen
To clarify the antibiotic resistance mechanisms of Myroides odoratimimus, pan-resistant M. odoratimimus strain PR63039 was isolated and its genome sequenced and analyzed. Antimicrobial susceptibility testing was conducted using the Kirby-Bauer disk diffusion method, and the Phoenix-100 Automated Microbiology System with a NMIC/ID-4 panel including aminoglycosides, β-lactams, polypeptides, quinolones, sulfonamides, chloramphenicols, and tetracyclines. Single-molecule real-time whole genome sequencing was conducted using the PacBio RSII system, and genome annotation was performed using RAST and IMG ER...
September 12, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28878510/antimicrobial-activity-of-essential-oil-and-aqueous-and-ethanol-extracts-of-teucrium-polium-l-subsp-gabesianum-l-h-from-tunisia
#11
Mahmoud Ben Othman, Karima Bel Hadj Salah-Fatnassi, Saida Ncibi, Amer Elaissi, Lazhar Zourgui
The antimicrobial effects of essential oil, ethanol and aqueous extracts of Teucrium polium L. were investigated against 13 microorganisms. Extracts and essential oil were obtained from maceration, decoction and hydrodistillation respectively. Samples were tested for their antimicrobial activity using the disk diffusion, the agar dilution and the agar incorporation method. Essential oil was analysed using GC/MS, results showed that β-pinene (35.97%) and α-pinene (13.32%) were the main components. Furthermore, essential oil exhibited the highest antimicrobial activity, it was most effective against Proteus mirabilis, Staphylococcus aureus and Citrobacter freundei where inhibition zone ranged between 15 and 25 mm, and with the microbial inhibitory concentration (MIC) values of 0...
July 2017: Physiology and Molecular Biology of Plants: An International Journal of Functional Plant Biology
https://www.readbyqxmd.com/read/28869798/antimicrobial-activity-of-plant-extracts-against-the-honeybee-pathogens-paenibacillus-larvae-and-ascosphaera-apis-and-their-topical-toxicity-to-apis-mellifera-adults
#12
Veeranan Chaimanee, Ubonwan Thongtue, Nantaporn Sornmai, Sineenard Songsri, Jeffery S Pettis
AIMS: To explore alternative non-chemical control measures against two honeybee pathogens, Paenibacillus larvae and Ascosphaera apis, thirty-seven plant species were screened for antimicrobial activity. METHODS AND RESULTS: The activity of selected plant extracts was screened using a in vitro disk diffusion assay and the minimal inhibitory concentration (MIC) was determined by the broth microdilution method. The results showed that thirty-six plant extracts had some antibacterial activity on P...
September 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28869295/the-clinical-potential-of-senolytic-drugs
#13
James L Kirkland, Tamara Tchkonia, Yi Zhu, Laura J Niedernhofer, Paul D Robbins
Senolytic drugs are agents that selectively induce apoptosis of senescent cells. These cells accumulate in many tissues with aging and at sites of pathology in multiple chronic diseases. In studies in animals, targeting senescent cells using genetic or pharmacological approaches delays, prevents, or alleviates multiple age-related phenotypes, chronic diseases, geriatric syndromes, and loss of physiological resilience. Among the chronic conditions successfully treated by depleting senescent cells in preclinical studies are frailty, cardiac dysfunction, vascular hyporeactivity and calcification, diabetes mellitus, liver steatosis, osteoporosis, vertebral disk degeneration, pulmonary fibrosis, and radiation-induced damage...
October 2017: Journal of the American Geriatrics Society
https://www.readbyqxmd.com/read/28861286/comparison-of-periodontitis-associated-oral-biofilm-formation-under-dynamic-and-static-conditions
#14
Won Sub Song, Jae-Kwan Lee, Se Hwan Park, Heung-Sik Um, Si Young Lee, Beom-Seok Chang
PURPOSE: The purpose of this study was to compare the characteristics of single- and dual-species in vitro oral biofilms made by static and dynamic methods. METHODS: Hydroxyapatite (HA) disks, 12.7 mm in diameter and 3 mm thick, were coated with processed saliva for 4 hours. The disks were divided into a static method group and a dynamic method group. The disks treated with a static method were cultured in 12-well plates, and the disks in the dynamic method group were cultured in a Center for Disease Control and Prevention (CDC) biofilm reactor for 72 hours...
August 2017: Journal of Periodontal & Implant Science
https://www.readbyqxmd.com/read/28846710/apparent-diffusion-coefficient-maps-in-the-assessment-of-surgical-patients-with-lumbar-spine-degeneration
#15
Evgenii Belykh, Andrey A Kalinin, Arpan A Patel, Eric J Miller, Michael A Bohl, Ivan A Stepanov, Liudmila A Bardonova, Talgat Kerimbaev, Anton O Asantsev, Morgan B Giers, Mark C Preul, Vadim A Byvaltsev
PURPOSE: To assess the utility of apparent diffusion coefficient (ADC) maps for the assessment of patients with advanced degenerative lumbar spine disease and describe characteristic features of ADC maps in various degenerative lumbar spinal conditions. METHODS: T1-weighted, T2-weighted and diffusion weighted (DWI) MR images of 100 consecutive patients admitted to the spinal surgery service were assessed. ADC maps were generated from DWI images using Osyrix software...
2017: PloS One
https://www.readbyqxmd.com/read/28830451/bacterial-pathogens-associated-with-external-ocular-infections-alongside-eminent-proportion-of-multidrug-resistant-isolates-at-the-university-of-gondar-hospital-northwest-ethiopia
#16
Ergibnesh Getahun, Baye Gelaw, Abate Assefa, Yared Assefa, Anteneh Amsalu
BACKGROUND: External ocular infection is a public health problem in Ethiopia. The aim of this study was to determine the prevalence and antimicrobial susceptibility pattern of external ocular bacterial infections. METHODS: A cross sectional study was conducted at the University of Gondar Hospital among patients seeking health services at the Department of Ophthalmology from January to April, 2016. All patients with suspected external ocular infections were examined under slit lamp microscope...
August 22, 2017: BMC Ophthalmology
https://www.readbyqxmd.com/read/28820764/severe-case-of-renal-coloboma-syndrome-in-long-term-follow-up
#17
Lise Sels, Werner Dirven, Koenraad Devriendt, Anita Leys
PURPOSE: To characterize the ocular features of a severe case of renal coloboma syndrome in a long-term follow-up. METHODS: Observational case report over a period of 45 years. Examination under anesthesia at the age of 3 months, repeated ophthalmologic examination (age 7, 14, 25, 45 years), fluorescein and indocyanine green angiography, electroretinography, ocular ultrasound, optical coherence tomography, computed tomography scan orbits, and magnetic resonance imaging of the brain...
August 17, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28816862/unilateral-optic-neuritis-and-central-retinal-vasculitis-due-to-ocular-syphilis
#18
Murtaza S Khan, Dulanji K Kuruppu, Tanav A Popli, Ramana S Moorthy, Devin D Mackay
PURPOSE: Report a case of concurrent unilateral optic neuritis and central retinal artery occlusion as the presenting signs of syphilis. METHODS: A case report of a 22-year-old man with progressive unilateral vision loss. RESULTS: With no known previous history of syphilis, genital lesions, or other extraocular manifestations, the patient presented with pain with eye movements and decreased color vision. His vision dramatically worsened after a course of oral steroids...
August 14, 2017: Retinal Cases & Brief Reports
https://www.readbyqxmd.com/read/28812009/the-bacteriological-quality-safety-and-antibiogram-of-salmonella-isolates-from-fresh-meat-in-retail-shops-of-bahir-dar-city-ethiopia
#19
Melkamnesh Azage, Mulugeta Kibret
The habit of raw meat consumption in addition to the poor hygienic standards and lack of knowledge contribute to food-borne diseases outbreaks. The objective of this research was to assess the bacterial quality and safety of fresh meat from retail Bahir Dar City, Ethiopia. A total of 30 fresh meat samples were collected from butcher shops. Standard bacteriological methods were used to isolate and enumerate bacteria. Kirby-Bauer disk diffusion method was used for antimicrobial susceptibility testing of Salmonella isolates...
2017: International journal of food science
https://www.readbyqxmd.com/read/28802761/ultrasound-versus-magnetic-resonance-imaging-of-the-temporomandibular-joint-in-juvenile-idiopathic-arthritis-a-systematic-review
#20
REVIEW
B L Hechler, J A Phero, H Van Mater, N S Matthews
A systematic review of published articles on ultrasound (US) and magnetic resonance imaging (MRI) of the temporomandibular joint (TMJ) in juvenile idiopathic arthritis (JIA) was performed to answer the question "What is the sensitivity and specificity of US as compared to MRI in diagnosing acute and chronic joint changes in patients with JIA?" The most recent evidence was sought in published articles via a search of the PubMed, Ovid, and Embase databases. Article appraisal was performed by two reviewers. Nineteen articles reporting prospective or ambispective studies comparing US to MRI in TMJ imaging were found...
August 9, 2017: International Journal of Oral and Maxillofacial Surgery
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