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Mucin 5ac

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https://www.readbyqxmd.com/read/27912076/activating-transcription-factor-3-is-essential-for-cigarette-smoke-induced-mucin-expression-via-interaction-with-activator-protein-1
#1
Yan-Ping Wu, Yin-Fang Wu, Chao Zhang, Hong-Bin Zhou, Chao Cao, Miao Li, Chen Zhu, Song-Min Ying, Zhi-Hua Chen, Hua-Hao Shen, Wen Li
Mucus hypersecretion is an important pathologic feature of chronic obstructive pulmonary disease. Activating transcription factor 3 (ATF3) is an adaptive-response gene that participates in various cellular processes. However, little is known about its role in cigarette smoke (CS)-induced mucus hyperproduction. This study aimed to investigate the role and molecular mechanisms of ATF3 in CS-induced Mucin 5AC (MUC5AC) expression. ATF3 was elevated in lung tissues of mice exposed to CS for 12 weeks. Treatment with CS extract significantly induced ATF3 expression and MUC5AC production in human bronchial epithelial cells, NCI-H292, and mouse tracheal epithelial cells...
November 29, 2016: American Journal of Pathology
https://www.readbyqxmd.com/read/27795812/mucins-in-neoplasms-of-pancreas-ampulla-of-vater-and-biliary-system
#2
REVIEW
Dimitrios Moschovis, Giorgos Bamias, Ioanna Delladetsima
Tumors of the pancreas, the ampulla of Vater, and the extrahepatic and intrahepatic bile ducts have significant histological similarities due to the common embryonic origin of the pancreatobiliary system. This obviates the need for discovery of biomarkers with diagnostic and prognostic value for these tumors. Mucins, especially MUC-1, -2, -4 and -5AC, are important candidates for developing into such reliable biomarkers. Increased expression of MUC1 occurs in pancreatic ductal adenocarcinomas and is associated with increased degrees of dysplasia in pancreatic intraepithelial neoplasia (PanIN)...
October 15, 2016: World Journal of Gastrointestinal Oncology
https://www.readbyqxmd.com/read/27780139/platycodin-d-inhibits-interleukin-13-induced-the-expression-of-inflammatory-cytokines-and-mucus-in-nasal-epithelial-cells
#3
Botao Wang, Ying Gao, Guoxi Zheng, Xiaoyong Ren, Bin Sun, Kang Zhu, Huanan Luo, Zhenghui Wang, Min Xu
Allergic rhinitis (AR) is a common chronic inflammatory condition of the nasal mucosal tissue. Platycodin D (PLD), a triterpenoid saponin isolated from the root of Platycodon grandiflorum, has anti-inflammatory effects in a mouse model of allergic asthma. However, the anti-inflammatory effects of PLD in the nasal mucosa have not been deeply investigated. The objective was to investigate the effect of PLD on inflammatory cytokines and mucus production from nasal epithelial cells. Our study showed that PLD inhibited the levels of granulocyte-macrophage colony-stimulating factor (GM-CSF) and eotaxin in interleukin (IL)-13-stimulated RPMI2650 cells...
October 22, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/27645929/astragalus-polysaccharide-modulates-er-stress-response-in-an-ova-lps-induced-murine-model-of-severe-asthma
#4
Yuan Lu, Qiong-Qiong Xing, Jian-Ya Xu, Dou Ding, Xia Zhao
Endoplasmic reticulum (ER) stress has been recently revealed to play a pivotal role in the pathogenesis of severe asthma. Astragalus polysaccharide (APS), a major bioactive component from Astragalus membranaceus, exerts immunomodulatory and anti-inflammatory effects and has been shown to suppress ER stress in chronic diseases such as type-2 diabetes. However, the pharmaceutical application of APS in the treatment of severe asthma is unknown. The results obtained here indicate that APS significantly attenuates eosinophils and neutrophil-dominant airway inflammation by reducing the mRNA levels of Cxcl5, Il8, and chemokine (C-C motif) ligand 20 (Ccl20) and the protein levels of IL13RA and IL17RA...
September 16, 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27588910/interleukin-13-stimulates-muc5ac-expression-via-a-stat6-tmem16a-erk1-2-pathway-in-human-airway-epithelial-cells
#5
Yanling Qin, Youfan Jiang, Awais Shafiq Sheikh, Shanshan Shen, Jing Liu, Depeng Jiang
Transmembrane protein 16A (TMEM16A), a channel underlying the calcium-activated chloride channel (CaCC) currents, has been shown to be a key regulator of mucus overproduction in airway epithelial cells. However, the precise molecular mechanism involved in the TMEM16A-mediated mucus secretion remains unclear. In the present study, we inquired into a novel signaling mechanism for TMEM16A driving mucin 5AC (MUC5AC) production in human airway epithelial cells. Following treatment for 24-48h with type 13 interleukin (IL-13), an upregulation of TMEM16A expression in both mRNA and protein levels was observed in human bronchial epithelial cell line (HBE16), while signal transducer and activator of transcription 6 (STAT6) inhibition could decrease this elevated expression, suggesting that the regulation of TMEM16A expression by IL-13 was via a STAT6-based transcriptional mechanism...
August 30, 2016: International Immunopharmacology
https://www.readbyqxmd.com/read/27430907/pinellia-ternata-attenuates-mucus-secretion-and-airway-inflammation-after-inhaled-corticosteroid-withdrawal-in-copd-rats
#6
Wei Du, Jinyu Su, Dan Ye, Yuegang Wang, Qiaobing Huang, Xiaowei Gong
Inhaled corticosteroids (ICS) are widely used to manage chronic obstructive pulmonary disease (COPD). However, withdrawal of ICS generally causes various adverse effects, warranting careful management of the ICS withdrawal. Pinellia ternata, a traditional Chinese herbal medicine, has been used to treat respiratory diseases in China for centuries. Here, we investigated its role in antagonizing ICS withdrawal-induced side effects, and explored the underlying mechanisms. The rat COPD model was established using a combination of passive cigarette smoking and intratracheal instillation of lipopolysaccharide (LPS)...
2016: American Journal of Chinese Medicine
https://www.readbyqxmd.com/read/27391093/characterization-of-goblet-cells-in-a-pterygium-biopsy-using-laser-scanning-confocal-microscopy-and-immunohistochemistry
#7
Luisa H Colorado, Nicola Pritchard, Brendan G Cronin, Nathan Efron
PURPOSE: To confirm that structures presumed to be GCs observed using laser scanning confocal microscopy (LSCM) are actually GCs. METHODS: A single tissue sample was obtained from a pterygium that was freshly excised from a 33-year-old male. After viewing what were believed to be GCs in the tissue sample using LSCM, the same sample was observed using laboratory confocal microscopy and immunohistochemistry. GCs were identified by a combination of classic morphologic appearance and the use of immunofluorescence to antibodies for mucin 5AC and cytokeratin-7...
August 2016: Cornea
https://www.readbyqxmd.com/read/27349904/the-applicability-of-elisa-detection-of-gastric-mucosa-expressing-proteins-for-the-identification-of-vomit
#8
Tomoko Akutsu, Hisako Saito, Hirotaro Iwase, Ken Watanabe, Ayari Takamura, Koichi Sakurada, Sachio Miyasaka
The identification of vomit stains may be helpful for crime scene reconstruction. However, there is no specific and convenient method for identifying vomit stain. Therefore, to establish the procedure for forensic identification of vomit stains, we focused on four gastric mucosa-expressing proteins, pepsinogen I (PGA), pepsinogen II (PGC), gastrin (GAST), and mucin 5AC (MUC5AC). We developed enzyme-linked immunosorbent assay (ELISA) procedures for the detection of these four candidate proteins. The specificity and sensitivity of ELISA detection of these proteins were analyzed, and applicability for the identification of vomit in forensic casework samples was also investigated...
June 27, 2016: International Journal of Legal Medicine
https://www.readbyqxmd.com/read/27347157/differential-expression-of-mucins-in-middle-eastern-patients-with-colorectal-cancer
#9
Khayal Al-Khayal, Maha Abdulla, Omar Al-Obaid, Ahmad Zubaidi, Mansoor-Ali Vaali-Mohammed, Abdulmalik Alsheikh, Rehan Ahmad
Mucin overexpression has been implicated in the tumorigenesis and progression of colorectal carcinoma (CRC). However, data obtained on the prognostic importance of mucin expression in CRC is inconsistent. Due to lack of data on mucin expression and the increase in CRC incidence in Saudi Arabia, the aim of the present study was to analyze the mucin expression profile in patients with CRC in this ethnic group. The present study consisted of 22 patients that underwent surgery for CRC. Histopathological and immunohistochemical staining was performed on CRC tumor and adjacent normal tissues...
July 2016: Oncology Letters
https://www.readbyqxmd.com/read/27334783/synergistic-effect-of-dermatophagoides-farinae-and-lipopolysaccharides-in-human-middle-ear-epithelial-cells
#10
Ji Eun Lee, Yeon Hoo Kim, Chae Seo Rhee, Dong Young Kim
PURPOSE: Although the concept of "one airway, one disease," which includes the middle ear space as part of the united airway is well recognized, the role of allergens in otitis media with effusion (OME) is not clearly understood. We aimed to investigate the effect of the interaction between Dermatophagoides farinae (Der f) and lipopolysaccharide (LPS) on the induction of epithelial inflammatory response in vitro. METHODS: Primary human middle ear epithelial cells were exposed to Der f, LPS, or both in different sequences, and the magnitude of the immunologic responses was compared...
September 2016: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/27296651/increased-expression-of-hclca1-in-chronic-rhinosinusitis-and-its-contribution-to-produce-muc5ac
#11
Ha Kyun Kim, Jin Ho Kook, Ka Ram Kang, Dong Ju Oh, Tae Hoon Kim, Sang Hag Lee
OBJECTIVES/HYPOTHESIS: Mucus hypersecretion is a hallmarks of chronic rhinosinusitis. The expression of MUC5AC, a major respiratory mucin gene, is increased in chronic rhinosinusitis. The mechanisms inducing mucus hypersecretion have not been fully evaluated in chronic rhinosinusitis. Human Ca(2+) -activated Cl- channel 1 (hCLCA1) is implicated in the regulation of mucus production, airway fluid, and electrolyte transport. The present study objectives was to investigate the expression of hCLCA1 in chronic rhinosinusitis and evaluate whether its level is altered by stimulation with type 1 T helper (Th1) and Th2 cytokines, and to determine the possible role of hCLCA1 on the regulation of mucin 5AC (MUC5AC) production...
June 14, 2016: Laryngoscope
https://www.readbyqxmd.com/read/27095518/asian-sand-dust-enhances-the-inflammatory-response-and-mucin-gene-expression-in-the-middle-ear
#12
Jiwon Chang, Yoon Young Go, Moo Kyun Park, Sung-Won Chae, Seon-Heui Lee, Jae-Jun Song
OBJECTIVES: Asia sand dust (ASD) is known to cause various human diseases including respiratory infection. The aim of this study was to examine the effect of ASD on inflammatory response in human middle ear epithelial cells (HMEECs) in vitro and in vivo. METHODS: Cell viability was assessed using the cell counting kit-8 assay. The mRNA levels of various genes including COX-2, TNF-a, MUC 5AC, MUC 5B, TP53, BAX, BCL-2, NOX4, and SOD1 were analyzed using semiquantitative realtime polymerase chain reaction...
September 2016: Clinical and Experimental Otorhinolaryngology
https://www.readbyqxmd.com/read/27075970/regulation-of-viral-infection-induced-airway-remodeling-cytokine-production-by-the-tlr3-egfr-signaling-pathway-in-human-bronchial-epithelial-cells
#13
Jingjin Jiang, Yuehong Wang, Xiaoping Tang, Yinan Yao, Jianying Zhou
Toll-like receptor 3 (TLR3) is involved in the virus-induced pulmonary inflammatory response, but its role in airway remodeling after viral infection is unclear. We explored the role of TLR3 in poly(I:C)-induced inflammatory cytokines and mucin 5AC (MUC5AC) production in human bronchial epithelial cells by Western blotting, RT-PCR and ELISA. The expression of TLR3, MUC5AC, Matrixmetalloproteinase (MMP9), Transforming growth factor (TGF-β1) and Vascular endothelial growth factor (VEGF) in human bronchial epithelial cells increased in a dose-dependent manner after exposure to poly(I:C), and this effect was inhibited by treatment with TLR3 siRNA...
December 2016: COPD
https://www.readbyqxmd.com/read/26819423/-effect-of-aminophylline-and-simvastatin-on-airway-inflammation-and-mucus-hypersecretion-in-rats-with-chronic-obstructive-pulmonary-disease
#14
Sheng Wang, Lingling Xiong, Xue Deng, Qun Zhou, Chunying Li, Wei Ren, Chundong Zhu
OBJECTIVE: To observe the role of aminophylline and simvastatin in preventing and curing chronic obstructive pulmonary disease (COPD), and to explore the underlying mechanisms based on airway inflammation and mucus hypersecretion.
 METHODS: The rat model of COPD was established by combination of cigarette smoking with intratracheal lipopolysaccharide (LPS) injection. Male SD rats were randomly divided into 4 groups (n=10 per group): a control group, a COPD group, an aminophylline group and a simvastatin group...
January 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/26819418/-mediated-mechanism-of-ndrg2-in-the-regulation-of-neutrophil-nuclear-transcription-factor-%C3%AE%C2%BAb-signaling-pathway-through-the-deubiquitylating-enzyme-of-zc3h12d
#15
Mengmeng Shi, Qi Li, Xiangdong Zhou, Huaping Huang, Jie Wang
OBJECTIVE: To explore the role NDRG2/Zc3h12d in the regulation of neutrophil nuclear transcription factor-κB signaling pathway and the underlying mechanisms.
 METHODS: Sixteen HBE cells were incubated with lipopolysaccharide (LPS) to establish airway mucus hypersecretion model, which was transfected with NDRG2 or Zc3h12d siRNA. The cells were divided into 5 groups: a LPS+NDRG2 siRNA (Group A), a LPS+ NDRG2 and Zc3h12d siRNAs (Group B), a LPS+Zc3h12d siRNA (Group C), a LPS+ empty plasmid (Group D), and a negative control group (Group E)...
January 2016: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/26735541/hyper-expression-of-mucin-5ac-indicates-poor-cancer-prognoses-a-meta-analysis
#16
REVIEW
Xin Wang, Fei Yan, Run Shi, Xing Huang, Shiming Lu, Lin Xu, Binhui Ren
The aim of the study was to explore the association between mucin 5ac expression and cancer prognosis. A systematically comprehensive search was performed through PubMed, the Web of Science, and the China National Knowledge Infrastructure (CNKI). The prognostic value of mucin 5ac expression in cancer patients was evaluated. The overexpression of mucin 5ac was found to be significantly associated with a poor prognosis in cancer patients (pooled HR: 1.53, 95%CI: 1.158-2.028, P = 0.003). This association was also detected in a biliary subgroup (pooled HR: 1...
January 2016: Medicine (Baltimore)
https://www.readbyqxmd.com/read/26714961/cytoreductive-surgery-plus-hyperthermic-intraperitoneal-chemotherapy-for-pseudomyxoma-peritonei-arising-from-urachus
#17
Yang Liu, Haruaki Ishibashi, Masamitsu Hirano, Kazuyoshi Takeshita, Akiyoshi Mizumoto, Masumi Ichinose, Eisei Nishino, Ippei Kashu, Yoshihiro Yamamoto, Paul H Sugarbaker, Yutaka Yonemura
BACKGROUND: Pseudomyxoma peritonei (PMP) is a rare locoregional disease characterized by disseminated intraperitoneal mucinous tumors. However, little is known about PMP from urachal neoplasm as a result of its rarity. METHODS: A total of 9 patients with PMP of urachal origin were treated by cytoreductive surgery (CRS) plus hyperthermic intraperitoneal chemotherapy (HIPEC) in our institution. All specimens of surgeries were submitted for pathologic examination. Representative slides of tumors and normal urachus were submitted for immunohistochemical staining...
December 29, 2015: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/26549244/resveratrol-inhibits-mucus-overproduction-and-muc5ac-expression-in-a-murine-model-of-asthma
#18
Zhen-Hua Ni, Ji-Hong Tang, Guo Chen, Yi-Min Lai, Qing-Ge Chen, Zao Li, Wei Yang, Xu-Min Luo, Xiong-Biao Wang
Previous in vitro studies have demonstrated that resveratrol is able to significantly inhibit the upregulation of mucin 5AC (MUC5AC), a major component of mucus; thus indicating that resveratrol may have potential in regulating mucus overproduction. However, there have been few studies regarding the resveratrol‑mediated prevention of MUC5AC overproduction in vivo, and the mechanisms by which resveratrol regulates MUC5AC expression have yet to be elucidated. In the present study, an ovalbumin (OVA)‑challenged murine model of asthma was used to assess the effects of resveratrol treatment on mucus production in vivo...
January 2016: Molecular Medicine Reports
https://www.readbyqxmd.com/read/26511846/immunohistochemical-detection-of-markers-for-translational-studies-of-lung-disease-in-pigs-and-humans
#19
David K Meyerholz, Allyn M Lambertz, Leah R Reznikov, Georgina K Ofori-Amanfo, Phil H Karp, Paul B McCray, Michael J Welsh, David A Stoltz
Genetically engineered pigs are increasingly recognized as valuable models for the study of human disease. Immunohistochemical study of cellular markers of disease is an important tool for the investigation of these novel models so as to evaluate genotype and treatment differences. Even so, there remains a lack of validated markers for pig tissues that can serve as a translational link to human disease in organs such as the lung. Herein, we evaluate markers of cellular inflammation (cluster of differentiation [CD]3, CD79a, B cell lymphoma [BCL] 6, ionized calcium-binding adapter molecule [IBA]1, and myeloperoxidase) and those that may be involved with tissue remodeling (alpha-smooth muscle actin, beta-tubulin-III, lactoferrin, mucin [MUC]5AC, MUC5B, and cystic fibrosis transmembrane conductance regulator [CFTR]) for study of lung tissues...
April 2016: Toxicologic Pathology
https://www.readbyqxmd.com/read/26471792/gaba-selectively-increases-mucin-1-expression-in-isolated-pig-jejunum
#20
Hannah-Sophie Braun, Gerhard Sponder, Robert Pieper, Jörg R Aschenbach, Carolin Deiner
The inhibitory neurotransmitter GABA (γ-aminobutyric acid) is synthesized by glutamic acid decarboxylase, which is expressed in the central nervous system and in various other tissues including the intestine. Moreover, GABA can be ingested in vegetarian diets or produced by bacterial commensals in the gastrointestinal tract. As previous studies in lung have suggested a link between locally increased GABA availability and mucin 5AC production, the present study sought to test whether the presence or lack of GABA (and its precursor glutamine) has an effect on intestinal mucin expression...
November 2015: Genes & Nutrition
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