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https://www.readbyqxmd.com/read/29782826/sheets-of-human-retinal-progenitor-transplants-improve-vision-in-rats-with-severe-retinal-degeneration
#1
Bin Lin, Bryce T McLelland, Anuradha Mathur, Robert B Aramant, Magdalene J Seiler
Loss of photoreceptors and other retinal cells is a common endpoint in retinal degenerate (RD) diseases that cause blindness. Retinal transplantation is a potential therapy to replace damaged retinal cells and improve vision. In this study, we examined the development of human fetal retinal sheets with or without their retinal pigment epithelium (RPE) transplanted to immunodeficient retinal degenerate rho S334ter-3 rats. Sheets were dissected from fetal human eyes (11-15.7 weeks gestation) and then transplanted to the subretinal space of 24-31 d old RD nude rats...
May 18, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29780649/sonographic-demonstration-of-intracranial-hemorrhage-in-a-fetus-with-hydrops-fetalis-due-to-rh-alloimmunization-after-intrauterine-intravascular-transfusion-a-case-report-and-review-of-the-literature
#2
Rauf Melekoglu, Ebru Celik, Hasim Kural
Intrauterine transfusion is the most common and successful intrauterine procedure for the treatment of fetal anemia due to red cell alloimmunization. Fetal intracranial hemorrhage is a very rare complication of intrauterine transfusion in patients with Rh(D) alloimmunization and it has been demonstrated only in a few case reports in the literature. Herein, we described a case of grade IV intraventricular hemorrhage that was diagnosed following the first intrauterine transfusion and reviewed the literature about the fetal intracranial hemorrhage that occurred after intrauterine intravascular transfusion procedure...
2018: Case Reports in Obstetrics and Gynecology
https://www.readbyqxmd.com/read/29779467/calcium-sodium-and-trp-channel-expression-in-human-fetal-midbrain-derived-neural-progenitor-cells
#3
Nancy Stanslowsky, Salini Tharmarasa, Selma Staege, Norman Kalmbach, Martin Klietz, Sigrid Schwarz, Andreas Leffler, Florian Wegner
Voltage-gated sodium and calcium channels as well as transient receptor potential (TRP) channels are expressed during the differentiation of human neural progenitor cells (hNPCs) and are likely to be involved in regulating neurogenesis. However, the molecular composition of these ion channels in proliferating and differentiating hNPCs is largely unknown. Here, we investigated fetal mesencephalic hNPCs in respect to their sodium, calcium, and TRP channel subunit expression and function. Quantitative real-time PCR indicated a significant upregulation of voltage-gated sodium and calcium channel subunits in hNPCs after differentiation for 3 weeks in vitro...
May 19, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29778942/transcriptomic-and-methylomic-analysis-reveal-the-toxicological-effect-of-2-3-7-8-tetrachlorodibenzodioxin-on-human-embryonic-stem-cell
#4
Keng Po Lai, Jing Woei Li, Ting Fung Chan, Andy Chen, Cherie Yin Lau Lee, William Shu Biu Yeung, Chris Kong Chu Wong
Cumulating epidemiological studies demonstrated that environmental exposure to endocrine disrupting chemicals (EDCs) during the early stages of fetal development is associated with the increase in disease susceptibility in later life. The fetal developmental plasticity is considered as a protective mechanism against an undesirable prenatal environment. Dioxin is one of the environmental contaminants and is considered a diabetogenic factor. Experimental animal and human epidemiological studies have revealed that dioxin exposure was associated with insulin resistance and altered beta cell function...
May 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29778664/gestational-diabetes-mellitus-modulates-cholesterol-homeostasis-in-human-fetoplacental-endothelium
#5
Yidan Sun, Susanne Kopp, Jasmin Strutz, Chaitanya Chakravarthi Gali, Martina Zandl-Lang, Elham Fanaee-Danesh, Andrijana Kirsch, Silvija Cvitic, Saša Frank, Richard Saffery, Ingemar Björkhem, Gernot Desoye, Christian Wadsack, Ute Panzenboeck
Gestational diabetes mellitus (GDM) is associated with excessive oxidative stress which may affect placental vascular function. Cholesterol homeostasis is crucial for maintaining fetoplacental endothelial function. We aimed to investigate whether and how GDM affects cholesterol metabolism in human fetoplacental endothelial cells (HPEC). HPEC were isolated from fetal term placental arterial vessels of GDM or control subjects. Cellular reactive oxygen species (ROS) were detected by H2 DCFDA fluorescent dye. Oxysterols were quantified by gas chromatography-mass spectrometry analysis...
May 17, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29778312/biomarker-signatures-of-sickle-cell-disease-severity
#6
Mengtian Du, Sarah Van Ness, Victor Gordeuk, Sayed M Nouraie, Sergei Nekhai, Mark Gladwin, Martin H Steinberg, Paola Sebastiani
Identifying sickle cell disease patients at high risk of complications could lead to personalized treatment and better prognosis but despite many advances prediction of the clinical course of these patients remains elusive. We propose a system-type approach to discover profiles of multiple, common biomarkers that correlate with morbidity and mortality in sickle cell disease. We used cluster analysis to discover 17 signatures of 17 common circulating biomarkers in 2320 participants of the Cooperative Study of Sickle Cell Disease, and evaluated the association of these signatures with risk for stroke, pain, leg ulceration, acute chest syndrome, avascular necrosis, seizure, death, and trend of fetal hemoglobin and hemolysis using longitudinally collected data...
May 16, 2018: Blood Cells, Molecules & Diseases
https://www.readbyqxmd.com/read/29777389/iron-based-metal-organic-frameworks-as-a-theranostic-carrier-for-local-tuberculosis-therapy
#7
Gabriela Wyszogrodzka, Przemysław Dorożyński, Barbara Gil, Wieslaw J Roth, Maciej Strzempek, Bartosz Marszałek, Władysław P Węglarz, Elżbieta Menaszek, Weronika Strzempek, Piotr Kulinowski
PURPOSE: The purpose of the study was initial evaluation of applicability of metal organic framework (MOF) Fe-MIL-101-NH2 as a theranostic carrier of antituberculous drug in terms of its functionality, i.e. drug loading, drug dissolution, magnetic resonance imaging (MRI) contrast and cytotoxic safety. METHODS: Fe-MIL-101-NH2 was characterized using X-ray powder diffraction, FTIR spectrometry and scanning electron microscopy. The particle size analysis was determined using laser diffraction...
May 18, 2018: Pharmaceutical Research
https://www.readbyqxmd.com/read/29776850/what-is-the-best-protocol-to-cryopreserve-immature-mouse-testicular-cell-suspensions
#8
Jaime Onofre, Katrien Faes, Prashant Kadam, Elena Vicini, Ans M M van Pelt, Ellen Goossens
RESEARCH QUESTION: From a clinical perspective, which parameters grant optimal cryopreservation of mouse testicular cell suspensions? DESIGN: We studied the effect of different cryopreservation rates, the addition of sugars, different vessels and the addition of an apoptotic inhibitor on the efficiency of testicular cell suspension cryopreservation. After thawing and warming, testicular cell suspensions were transplanted to recipient mice for further functional assay...
April 27, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/29776631/molecular-pathology-in-transfusion-medicine-new-concepts-and-applications
#9
REVIEW
Matthew B Elkins, Robertson D Davenport, Martin H Bluth
Virtually all the red blood cell and platelet antigen systems have been characterized at the molecular level. Highly reliable methods for red blood cell and platelet antigen genotyping are now available. Genotyping is a useful adjunct to traditional serology and can help resolve complex serologic problems. Although red blood cell and platelet phenotypes can be inferred from genotype, knowledge of the molecular basis is essential for accurate assignment. Genotyping of blood donors is an effective method of identifying antigen-negative and/or particularly rare donors...
June 2018: Clinics in Laboratory Medicine
https://www.readbyqxmd.com/read/29774769/serum-mediated-activation-of-bone-marrow-derived-mesenchymal-stem-cells-in-ischemic-stroke-patients-a-novel-preconditioning-method
#10
Gyeong Joon Moon, Yeon Hee Cho, Dong Hee Kim, Ji Hee Sung, Jeong Pyo Son, Sooyoon Kim, Jae Min Cha, Oh Young Bang
Stroke induces complex and dynamic, local and systemic changes including inflammatory reactions, immune responses, and repair and recovery processes. Mesenchymal stem cells (MSCs) have been shown to enhance neurological recovery after stroke. We hypothesized that serum factors play a critical role in the activation of bone marrow (BM) MSCs after stroke such as by increasing proliferation, paracrine effects, and rejuvenation. Human MSCs (hMSCs) were grown in fetal bovine serum (FBS), normal healthy control serum (NS), or stroke patient serum (SS)...
January 1, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/29774532/the-fetus-at-the-tipping-point-modifying-the-outcome-of-fetal-asphyxia
#11
Simerdeep K Dhillon, Christopher A Lear, Robert Galinsky, Guido Wassink, Joanne O Davidson, Sandra Juul, Nicola J Robertson, Alistair J Gunn, Laura Bennet
Brain injury around birth is associated with nearly half of all cases of cerebral palsy. Although brain injury is multifactorial, particularly after preterm birth, acute hypoxia-ischaemia is a major contributor to injury. It is now well established that the severity of injury after HI is determined by a dynamic balance between injurious and protective processes. In addition, mothers who are at risk of premature delivery have high rates of diabetes and antepartum infection/inflammation and are almost universally given treatments such as antenatal glucocorticoids and magnesium sulphate to reduce the risk of death and complications after preterm birth...
May 17, 2018: Journal of Physiology
https://www.readbyqxmd.com/read/29774106/hematopoietic-restricted-ptpn11e76k-reveals-indolent-mpn-progression-in-mice
#12
Stefan P Tarnawsky, Wen-Mei Yu, Cheng-Kui Qu, Rebecca J Chan, Mervin C Yoder
Juvenile Myelomonocytic Leukemia (JMML) is a pediatric myeloproliferative neoplasm (MPN) that has a poor prognosis. Somatic mutations in Ptpn11 are the most frequent cause of JMML and they commonly occur in utero . Animal models of mutant Ptpn11 have probed the signaling pathways that contribute to JMML. However, existing models may inappropriately exacerbate MPN features by relying on non-hematopoietic-restricted Cre-loxP strains or transplantations into irradiated recipients. In this study we generate hematopoietic-restricted models of Ptpn11E76K-mediated disease using Csf1r-MCM and Flt3Cre...
April 24, 2018: Oncotarget
https://www.readbyqxmd.com/read/29774074/placenta-specific-drug-delivery-by-trophoblast-targeted-nanoparticles-in-mice
#13
Baozhen Zhang, Lunbo Tan, Yan Yu, Baobei Wang, Zhilong Chen, Jinyu Han, Mengxia Li, Jie Chen, Tianxia Xiao, Balamurali K Ambati, Lintao Cai, Qing Yang, Nihar R Nayak, Jian Zhang, Xiujun Fan
Rationale: The availability of therapeutics to treat pregnancy complications is severely lacking, mainly due to the risk of harm to the fetus. In placental malaria, Plasmodium falciparum- infected erythrocytes (IEs) accumulate in the placenta by adhering to chondroitin sulfate A (CSA) on the surfaces of trophoblasts. Based on this principle, we have developed a method for targeted delivery of payloads to the placenta using a synthetic placental CSA-binding peptide (plCSA-BP) derived from VAR2CSA, a CSA-binding protein expressed on IEs...
2018: Theranostics
https://www.readbyqxmd.com/read/29771870/hyperfibrinolysis-is-an-important-cause-of-early-hemorrhage-in-patients-with-acute-promyelocytic-leukemia
#14
Yu-Hua Song, Chun Qiao, Li-Chan Xiao, Run Zhang, Hua - Lu
BACKGROUND The objective of the current study was to guide the early clinical treatment strategies by assessing the recovery of abnormal coagulation in acute promyelocytic leukemia (APL) patients during induction therapy. MATERIAL AND METHODS Retrospective analysis was performed in 112 newly-diagnosed patients with APL during induction treatment. RESULTS The early death (ED) rate in our study was 5.36% and the main cause was fetal hemorrhage. The presence of bleeding symptoms was significantly correlated with low platelet and fibrinogen levels...
May 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29771299/at-the-crossroads-of-fate-somatic-cell-lineage-specification-in-the-fetal-gonad
#15
Emmi Rotgers, Anne Jørgensen, Humphrey Hung-Chang Yao
The reproductive endocrine systems are vastly different between male and female. This sexual dimorphism of endocrine milieu originates from sex-specific differentiation of the somatic cells in the gonads during fetal life. The majority of gonadal somatic cells arise from the adrenogonadal primordium. After separation of the adrenal and gonadal primordia, the gonadal somatic cells initiate sex-specific differentiation during gonadal sex determination with the specification of the supporting cell lineages: Sertoli cells in the testis vs...
May 15, 2018: Endocrine Reviews
https://www.readbyqxmd.com/read/29770989/vitrification-for-bovine-embryos-with-low-quality-grade
#16
Hiroshi Tajimi, Tsugumi Yamazaki, Syoko Oike, Tatsuyuki Yoshida, Konosuke Okada, Masashige Kuwayama, Hitoshi Ushijima
This study was conducted to examine the utility of vitrification for bovine embryos with low-quality grade, and simple cryoprotectants dilution method for practitioners. In Experiment 1, survival of frozen embryos was compared with that of vitrified embryos using minimum volume cooling (MVC). Then, vitrified embryos were used to confirm the optimum sucrose concentration in Experiment 2. The survival rates of embryos that had been vitrified following diluted cryoprotectants with the one-step in-straw method were compared with those of fresh control embryos in Experiment 3...
May 17, 2018: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/29769607/facs-seq-analysis-of-pax3-derived-cells-identifies-non-myogenic-lineages-in-the-embryonic-forelimb
#17
Arun J Singh, Chih-Ning Chang, Hsiao-Yen Ma, Stephen A Ramsey, Theresa M Filtz, Chrissa Kioussi
Skeletal muscle in the forelimb develops during embryonic and fetal development and perinatally. While much is known regarding the molecules involved in forelimb myogenesis, little is known about the specific mechanisms and interactions. Migrating skeletal muscle precursor cells express Pax3 as they migrate into the forelimb from the dermomyotome. To compare gene expression profiles of the same cell population over time, we isolated lineage-traced Pax3+ cells (Pax3 EGFP ) from forelimbs at different embryonic days...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769550/prenatal-alcohol-exposure-increases-the-susceptibility-to-develop-aggressive-prolactinomas-in-the-pituitary-gland
#18
Shaima Jabbar, Kenneth Reuhl, Dipak K Sarkar
Excess alcohol use is known to promote development of aggressive tumors in various tissues in human patients, but the cause of alcohol promotion of tumor aggressiveness is not clearly understood. We used an animals model of fetal alcohol exposure that is known to promote tumor development and determined if alcohol programs the pituitary to acquire aggressive prolactin-secreting tumors. Our results show that pituitaries of fetal alcohol-exposed rats produced increased levels of intra-pituitary aromatase protein and plasma estrogen, enhanced pituitary tissue growth, and upon estrogen challenge developed prolactin-secreting tumors (prolactinomas) that were hemorrhagic and often penetrated into the surrounding tissue...
May 16, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29769429/mechanisms-involved-in-the-development-of-the-small-intestine-mucosal-layer-in-postnatal-piglets
#19
T H Skrzypek, W Kazimierczak, H Skrzypek, J L Valverde Piedra, M M Godlewski, R Zabielski
The use of complementary visualization and measurement techniques allowed accurate description and quantification of changes in the intestinal mucosal architecture and provided a comprehensive outlook on the dynamics of remodelling and maturation processes of the mucosal layer taking place in the small intestine of piglets from birth to weaning. The aim of the study was to examine the early postnatal development of the small intestine in pigs. Three techniques were used: scanning electron microscopy (measurements of villus density and shape, height of enterocytes and microvilli, cell exfoliation, and location of extrusion zones), optical microscopy (cross section, measurement of structures: villus length and width; crypt depth; mucosal thickness), and confocal microscopy (cell localization, apoptosis, exfoliation and migration)...
February 2018: Journal of Physiology and Pharmacology: An Official Journal of the Polish Physiological Society
https://www.readbyqxmd.com/read/29769009/mesenchymal-stem-cell-research-in-veterinary-medicine
#20
Mudasir Bashir Gugjoo, Amarpal, Vikash Chandra, Mohd Yaqoob Wani, Kuldeep Dhama, Gutulla Taru Sharma
BACKGROUND: The stem cells due to their unique characteristic features like self-renewal, multiplication, immuno-modulation and differentiation, are seen and hoped to bring new horizons in the field of biology, in general and medicine, in particular. OBJECTIVE: Among various stem cell types, currently mesenchymal stem cells (MSCs) are extensively studied in regenerative medicine owing to their readily available sources, easy harvesting and ability to differentiate both into mesodermal as well as non-mesodermal tissues with little associated ethical issues...
May 16, 2018: Current Stem Cell Research & Therapy
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