Hui Zhao, Jiachen Li, Siyuan Feng, Lin Xu, Bin Yan, Chengjuan Li, Meisong Li, Yaxuan Wang, Yaxin Li, Lujie Liang, Dianrong Zhou, Jia Wan, Wenli Wang, Guo-Bao Tian, Bing Gu, Xi Huang
Rifampicin is the most powerful first-line antibiotic for tuberculosis, which is caused by Mycobacterium tuberculosis. Although accumulating evidence from sequencing data of clinical M. tuberculosis isolates suggested that mutations in the rifampicin-resistance-determining region (RRDR) are strongly associated with rifampicin resistance, the comprehensive characterization of RRDR polymorphisms that confer this resistance remains challenging. By incorporating I-SceI sites for I-SceI-based integrant removal and utilizing an L5 swap strategy, we efficiently replaced the integrated plasmid with alternative alleles, making mass allelic exchange feasible in mycobacteria...
March 25, 2024: International Journal of Antimicrobial Agents