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A Clarner, W Uter, L Ruhmann, N Wrenger, A Martin, H Drexler
BACKGROUND: Psychological first aid is a common approach for providing support after potentially traumatic events (PTEs). In Germany, a peer support model is recommended by the German Statutory Accident Insurance (DGUV) to reduce sickness absence after occupational accidents, especially in public transportation. However, data on the effectiveness of peer support are very sparse. AIMS: To analyse whether peer support has an impact on sickness absence after work-related traumatic events in public transportation...
October 25, 2016: Occupational Medicine
Carla Brancia, Barbara Noli, Marina Boido, Andrea Boi, Roberta Puddu, Giuseppe Borghero, Francesco Marrosu, Paolo Bongioanni, Sandro Orrù, Barbara Manconi, Filomena D'Amato, Irene Messana, Federica Vincenzoni, Alessandro Vercelli, Gian-Luca Ferri, Cristina Cocco
VGF mRNA is widely expressed in areas of the nervous system known to degenerate in Amyotrophic Lateral Sclerosis (ALS), including cerebral cortex, brainstem and spinal cord. Despite certain VGF alterations are reported in animal models, little information is available with respect to the ALS patients. We addressed VGF peptide changes in fibroblast cell cultures and in plasma obtained from ALS patients, in parallel with spinal cord and plasma samples from the G93A-SOD1 mouse model. Antisera specific for the C-terminal end of the human and mouse VGF proteins, respectively, were used in immunohistochemistry and enzyme-linked immunosorbent assay (ELISA), while gel chromatography and HPLC/ESI-MS/MS were used to identify the VGF peptides present...
2016: PloS One
Matías Alvarez-Saavedra, Yves De Repentigny, Doo Yang, Ryan W O'Meara, Keqin Yan, Lukas E Hashem, Lemuel Racacho, Ilya Ioshikhes, Dennis E Bulman, Robin J Parks, Rashmi Kothary, David J Picketts
Exercise has been argued to enhance cognitive function and slow progressive neurodegenerative disease. Although exercise promotes neurogenesis, oligodendrogenesis and adaptive myelination are also significant contributors to brain repair and brain health. Nonetheless, the molecular details underlying these effects remain poorly understood. Conditional ablation of the Snf2h gene impairs cerebellar development producing mice with poor motor function, progressive ataxia, and death between postnatal days 25-45...
October 11, 2016: Cell Reports
Camila Maria da Silva Martinelli, André van Helvoort Lengert, Flavio Mavignier Cárcano, Eduardo Caetano Albino Silva, Mariana Brait, Luiz Fernando Lopes, Daniel Onofre Vidal
Testicular germ cell tumors (TGCT) represent the second main cause of cancer-related death in young men. Despite high cure rates, refractory disease results in poor prognosis. Epigenetic reprogramming occurs during the development of seminomas and non-seminomas. Understanding the molecular and genetic basis of these tumors would represent an important advance in the search for new TGCT molecular markers. Hence the frequency of methylation of a gene panel (VGF, MGMT, ADAMTS1, CALCA, HOXA9, CDKN2B, CDO1 and NANOG) was evaluated in 72 primary TGCT by quantitative methylation specific PCR...
August 10, 2016: Oncotarget
Anne-Li Lind, Payam Emami Khoonsari, Marcus Sjödin, Lenka Katila, Magnus Wetterhall, Torsten Gordh, Kim Kultima
OBJECTIVES: Electrical neuromodulation by spinal cord stimulation (SCS) is a well-established method for treatment of neuropathic pain. However, the mechanism behind the pain relieving effect in patients remains largely unknown. In this study, we target the human cerebrospinal fluid (CSF) proteome, a little investigated aspect of SCS mechanism of action. METHODS: Two different proteomic mass spectrometry protocols were used to analyze the CSF of 14 SCS responsive neuropathic pain patients...
August 2016: Neuromodulation: Journal of the International Neuromodulation Society
Soon Gang Choi, Qian Wang, Jingjing Jia, Maria Chikina, Hanna Pincas, Georgia Dolios, Kazuki Sasaki, Rong Wang, Naoto Minamino, Stephen R J Salton, Stuart C Sealfon
Reproductive function is controlled by the pulsatile release of hypothalamic gonadotropin-releasing hormone (GnRH), which regulates the expression of the gonadotropins luteinizing hormone and FSH in pituitary gonadotropes. Paradoxically, Fshb gene expression is maximally induced at lower frequency GnRH pulses, which provide a very low average concentration of GnRH stimulation. We studied the role of secreted factors in modulating gonadotropin gene expression. Inhibition of secretion specifically disrupted gonadotropin subunit gene regulation but left early gene induction intact...
September 30, 2016: Journal of Biological Chemistry
Ubadah Sabbagh, Saman Mullegama, Gerald J Wyckoff
The purpose of this study was to find genes linked with eating disorders and associated with both metabolic and neural systems. Our operating hypothesis was that there are genetic factors underlying some eating disorders resting in both those pathways. Specifically, we are interested in disorders that may rest in both sleep and metabolic function, generally called Night Eating Syndrome (NES). A meta-analysis of the Gene Expression Omnibus targeting the mammalian nervous system, sleep, and obesity studies was performed, yielding numerous genes of interest...
2016: BioMed Research International
Anne N Flynn, Michelle King, Mollie Rieff, Jill Krapf, Andrew T Goldstein
INTRODUCTION: Lichen sclerosus (LS) is a chronic inflammatory dermatosis, usually affecting the anogenital skin in women. This chronic inflammation can cause scarring of genitalia including narrowing of the introitus and phimosis of the clitoris. These architectural changes can lead to recurrent tearing during intercourse (vulvar granuloma fissuratum) and decreased clitoral sensation. Surgical correction of vulvar granuloma fissuratum (VGF) and clitoral phimosis can be performed, but there is little data on the patient satisfaction and complications following these surgical procedures...
December 2015: Sexual Medicine
Yang Lu, Xiaoniao Chen, Zhong Yin, Shuying Zhu, Di Wu, Xiangmei Chen
Mesangial proliferative nephritis (MesPGN) is a common kidney disease worldwide. The main feature of the disease is mesangial cell proliferation-induced injury to kidney function. In this study, we explored serum biomarkers for kidney function injury in anti-Thy1 nephritis. We found that mesangial proliferation were increased on days 5 and 7, and recovered by day 14 in anti-Thy1 nephritis. 24-h urine protein, the ratio of urine protein to urine creatine, serum creatine, and blood urea nitrogen, were increased at days 5 and 7 in the model...
March 2016: Proteomics
Grant D Foglesong, Wei Huang, Xianglan Liu, Andrew M Slater, Jason Siu, Vedat Yildiz, Stephen R J Salton, Lei Cao
Environmental enrichment (EE), a housing condition providing complex physical, social, and cognitive stimulation, leads to improved metabolic health and resistance to diet-induced obesity and cancer. One underlying mechanism is the activation of the hypothalamic-sympathoneural-adipocyte axis with hypothalamic brain-derived neurotrophic factor (BDNF) as the key mediator. VGF, a peptide precursor particularly abundant in the hypothalamus, was up-regulated by EE. Overexpressing BDNF or acute injection of BDNF protein to the hypothalamus up-regulated VGF, whereas suppressing BDNF signaling down-regulated VGF expression...
March 2016: Endocrinology
Viktória Koroknai, Szilvia Ecsedi, Laura Vízkeleti, Tímea Kiss, István Szász, Andrea Lukács, Orsolya Papp, Róza Ádány, Margit Balázs
Malignant melanoma is one of the most aggressive human cancers. Invasion of cells is the first step in metastasis, resulting in cell migration through tissue compartments. We aimed to evaluate genomic alterations specifically associated with the invasive characteristics of melanoma cells. Matrigel invasion assays were used to determine the invasive properties of cell lines that originated from primary melanomas. Array comparative genomic hybridization analyses were carried out to define the chromosome copy number alterations (CNAs)...
April 2016: Melanoma Research
Jo E Lewis, John M Brameld, Phil Hill, Dana Wilson, Perry Barrett, Francis J P Ebling, Preeti H Jethwa
The Siberian hamster (Phodopus sungorus) survives winter by decreasing food intake and catabolizing abdominal fat reserves, resulting in a sustained, profound loss of body weight. Hypothalamic tanycytes are pivotal for this process. In these cells, short-winter photoperiods upregulate deiodinase 3, an enzyme that regulates thyroid hormone availability, and downregulate genes encoding components of retinoic acid (RA) uptake and signaling. The aim of the current studies was to identify mechanisms by which seasonal changes in thyroid hormone and RA signaling from tanycytes might ultimately regulate appetite and energy expenditure...
February 2016: Journal of Molecular Endocrinology
Filomena D'Amato, Barbara Noli, Laura Angioni, Efisio Cossu, Michela Incani, Irene Messana, Barbara Manconi, Paola Solinas, Raffaella Isola, Stefano Mariotti, Gian-Luca Ferri, Cristina Cocco
To address the possible involvement of VGF peptides in obesity and diabetes, we studied type 2 diabetes (T2D) and obese patients, and high-fat diet induced obese mice. Two VGF peptides (NAPP-19 and QQET-30) were identified in human plasma by HPLC-ESI-MS. The VGF C-terminus, the above two cleaved peptides, and the TLQP-21 related peptide/s were studied using ELISA and immunohistochemistry. In euglycemic patients, plasma NAPPE and TLQP like peptides were significantly reduced with obesity (74±10 vs. 167±28, and 92±10 vs...
2015: PloS One
Barbara Noli, Carla Brancia, Roberta Pilleri, Filomena D'Amato, Irene Messana, Barbara Manconi, Francis J P Ebling, Gian-Luca Ferri, Cristina Cocco
VGF mRNA is induced in specific hypothalamic areas of the Siberian hamster upon exposure to short photoperiods, which is associated with a seasonal decrease in appetite and weight loss. Processing of VGF generates multiple bioactive peptides, so the objective of this study was to determine the profile of the VGF-derived peptides in the brain, pituitary and plasma from Siberian hamsters, and to establish whether differential processing might occur in the short day lean state versus long day fat. Antisera against short sequences at the C- or N- termini of proVGF, as well as against NERP-1, TPGH and TLQP peptides, were used for analyses of tissues, and both immunohistochemistry and enzyme linked immunosorbent assay (ELISA) coupled with high-performance liquid (HPLC) or gel chromatography were carried out...
2015: PloS One
Andrew M Hernandez, J Anthony Seibert, John M Boone
PURPOSE: Current dosimetry methods in mammography assume that the breast is comprised of a homogeneous mixture of glandular and adipose tissues. Three-dimensional (3D) dedicated breast CT (bCT) data sets were used previously to assess the complex anatomical structure within the breast, characterizing the statistical distribution of glandular tissue in the breast. The purpose of this work was to investigate the effect of bCT-derived heterogeneous glandular distributions on dosimetry in mammography...
November 2015: Medical Physics
P X Wang, S Shu, C Xia, Z Wang, L Wu, B Wang, C C Xu, J Liu
AIM: To determine differences in plasma proteomic profiles between healthy cows and those with subclinical hypocalcaemia within 12 hours after calving, and thereby explore the underlying biological mechanism of subclinical hypocalcaemia in dairy cows. METHODS: Plasma samples were collected within 6 hours of calving from Holstein cows on a farm in Heilongjiang, China; 32 with subclinical hypocalcaemia (plasma calcium concentration 1.38-2.00 mmol/L and no clinical signs) and 59 control cows (plasma calcium concentration 2...
March 2016: New Zealand Veterinary Journal
Valeria Bianchini, Laura Giusti, Maurizio Malavolta, Donatella Ussorio, Maria Grazia Cifone, Leila Fabiani, Massimo Casacchia, Rita Roncone, Rocco Pollice
AIM: Observational study on a young sample at the onset of affective disorder seeking help to a dedicated service, with the aim to evaluate the age of onset, the duration of untreated psychosis (DUP), and the functional outcome at 2-year follow-up. METHODS: For 57 young people at First Episode Psychosis (FEP) of Affective Disorder, consecutively recruited to SMILE service, were collected socio-demographic and clinical (DUP, psychiatric history, age of onset) data and psychopathological (BPRS e SCL-90), perceived distress (GHQ-12) and functioning (VGF) evaluations...
July 2015: Rivista di Psichiatria
Thierry Schmidlin, Arjen J Boender, Christian K Frese, Albert J R Heck, Roger A H Adan, A F Maarten Altelaar
Understanding regulation and action of endogenous peptides, especially neuropeptides, which serve as inter- and intracellular signal transmitters, is key in understanding a variety of functional processes, such as energy balance, memory, circadian rhythm, drug addiction, etc. Therefore, accurate and reproducible quantification of these bioactive endogenous compounds is highly relevant. The biosynthesis of endogenous peptides, involving multiple possible trimming and modification events, hinders the de novo prediction of the active peptide sequences, making MS-based measurements very valuable in determining the actual active compounds...
October 6, 2015: Analytical Chemistry
Mehran Rahimi, Manlio Vinciguerra, Mojtaba Daghighi, Behiye Özcan, Vishtaseb Akbarkhanzadeh, Fareeba Sheedfar, Marzyeh Amini, Tommaso Mazza, Valerio Pazienza, Mahdi M Motazacker, Morteza Mahmoudi, Felix W M De Rooij, Eric Sijbrands, Maikel P Peppelenbosch, Farhad Rezaee
Despite numerous developed drugs based on glucose metabolism interventions for treatment of age-related diseases such as diabetes neuropathies (DNs), DNs are still increasing in patients with type 1 or type 2 diabetes (T1D, T2D). We aimed to identify novel candidates in adipose tissue (AT) and pancreas with T2D for targeting to develop new drugs for DNs therapy. AT-T2D displayed 15 (e.g. SYT4 up-regulated and VGF down-regulated) and pancreas-T2D showed 10 (e.g. BAG3 up-regulated, VAV3 and APOA1 down-regulated) highly differentially expressed genes with neuronal functions as compared to control tissues...
October 6, 2015: Oncotarget
Jo E Lewis, John M Brameld, Phil Hill, Perry Barrett, Francis J P Ebling, Preeti H Jethwa
INTRODUCTION: The viral 2A sequence has become an attractive alternative to the traditional internal ribosomal entry site (IRES) for simultaneous over-expression of two genes and in combination with recombinant adeno-associated viruses (rAAV) has been used to manipulate gene expression in vitro. NEW METHOD: To develop a rAAV construct in combination with the viral 2A sequence to allow long-term over-expression of the vgf gene and fluorescent marker gene for tracking of the transfected neurones in vivo...
December 30, 2015: Journal of Neuroscience Methods
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