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https://www.readbyqxmd.com/read/28727044/muscle-lipid-metabolism-in-two-rabbit-lines-divergently-selected-for-intramuscular-fat
#1
M Martínez-Álvaro, S Agha, A Blasco, P Hernández
A divergent selection experiment for intramuscular fat (IMF) of LM at 9 wk of age was performed in rabbits. The objective of this work was to compare the lipid metabolism in muscles and fat tissues of the high-IMF and low-IMF lines. Lipogenic, catabolic, and lipolytic activities were studied in 2 muscles with different oxidative patterns (LM and semimembranosus proprius) and in the perirenal fat depot at 2 ages, 9 and 13 wk. In addition, adipocytes were characterized in perirenal fat. In the fifth generation, direct response to selection was 0...
June 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28713400/overexpression-of-e3-ubiquitin-ligase-gene-adbil-contributes-to-resistance-against-chilling-stress-and-leaf-mold-disease-in-tomato
#2
Shuangchen Chen, Hongjiao Zhao, Mengmeng Wang, Jidi Li, Zhonghong Wang, Fenghua Wang, Airong Liu, Golam J Ahammed
Ubiquitination is a common regulatory mechanism, playing a critical role in diverse cellular and developmental processes in eukaryotes. However, a few reports on the functional correlation between E3 ubiquitin ligases and reactive oxygen species (ROS) or reactive nitrogen species (RNS) metabolism in response to stress are currently available in plants. In the present study, the E3 ubiquitin ligase gene AdBiL (Adi3 Binding E3 Ligase) was introduced into tomato line Ailsa Craig via Agrobacterium-mediated method...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28700685/characterization-of-the-molecular-chaperone-clpb-from-the-pathogenic-spirochaete-leptospira-interrogans
#3
Joanna Krajewska, Anna Modrak-Wójcik, Zbigniew J Arent, Daniel Więckowski, Michal Zolkiewski, Agnieszka Bzowska, Sabina Kędzierska-Mieszkowska
Leptospira interrogans is a spirochaete responsible for leptospirosis in mammals. The molecular mechanisms of the Leptospira virulence remain mostly unknown. Recently, it has been demonstrated that an AAA+ chaperone ClpB (a member of the Hsp100 family) from L. interrogans (ClpBLi) is not only essential for survival of Leptospira under the thermal and oxidative stresses, but also during infection of a host. The aim of this study was to provide further insight into the role of ClpB in the pathogenic spirochaetes and explore its biochemical properties...
2017: PloS One
https://www.readbyqxmd.com/read/28647285/contrasting-patterns-of-energy-metabolism-in-northern-vs-southern-peripheral-european-flounder-populations-exposed-to-temperature-rising-and-hypoxia
#4
Nicolas Pédron, Jessy Le Du, Grégory Charrier, José-Luis Zambonino-Infante, Nicolas Le Bayon, Rita P Vasconcelos, Vanessa F Fonseca, Fabienne Le Grand, Jean Laroche
A two months common garden experiment was carried out to explore the potential differences of energy metabolism in northern core (France, 50°N and 47°N) vs southern peripheral (Portugal, 41°N) populations of European flounder Platichthys flesus, submitted to cold condition (CC: water temperature = 10 °C) and to warm and hypoxic condition (WHC: water temperature = 22 °C, and moderate hypoxia with O2 saturation = 40% during the last 6 days). Convergent growth rates (in length) were observed in the different populations and conditions, when the southern peripheral population of Portugal did not grow under cold conditions...
June 15, 2017: Marine Environmental Research
https://www.readbyqxmd.com/read/28633104/transcriptional-and-cellular-effects-of-paracetamol-in-the-oyster-crassostrea-gigas
#5
M J Bebianno, A C P Mello, M A S Serrano, F Flores-Nunes, J J Mattos, F L Zacchi, C E Piazza, M N Siebert, R S Piazza, C H A M Gomes, C M R Melo, A C D Bainy
Acetaminophen (paracetamol) (PAR) is one of the most popular non-steroidal anti-inflammatory drugs (NSAIDs) with analgesic and antipyretic properties consumed worldwide and often detected in the aquatic environment. Due to the fact that PAR induces oxidative stress in mammals, the aim of this study was to evaluate if similar effects were observed in oysters Crassostrea gigas, given their economic and ecological importance and worldwide distribution. Oysters were exposed for 1, 4 and 7 days to two different sublethal PAR concentrations (0, 1 and 100μgL(-1))...
June 17, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28609580/metabolic-defense-against-oxidative-stress-the-road-less-travelled-so-far
#6
REVIEW
Joe Lemire, Azhar Alhasawi, Varun P Appanna, Sujeenthar Tharmalingam, Vasu D Appanna
Bacteria have survived, and many have thrived, since antiquity in the presence of the highly-reactive chalcogen - oxygen (O2 ). They are known to evoke intricate strategies to defend themselves from the reactive by-products of oxygen - reactive oxygen species (ROS). Many of these detoxifying mechanisms have been extensively characterized; superoxide dismutase (SOD), catalases, alkyl hydroperoxide reductase and the glutathione (GSH)-cycling system are responsible for neutralizing specific ROS. Meanwhile, a pool of NADPH - the reductive engine of many ROS-combating enzymes - is maintained by metabolic enzymes including, but not exclusively, glucose-6 phosphate dehydrogenase (G6PDH) and NADP-dependent isocitrate dehydrogenase (ICDH-NADP)...
June 13, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28550410/different-exogenous-sugars-affect-the-hormone-signal-pathway-and-sugar-metabolism-in-red-globe-vitis-vinifera-l-plantlets-grown-in-vitro-as-shown-by-transcriptomic-analysis
#7
Juan Mao, Wenfang Li, Baoqin Mi, Mohammed Mujitaba Dawuda, Alejandro Calderón-Urrea, Zonghuan Ma, Yongmei Zhang, Baihong Chen
Exogenously applied 2% fructose is the most appropriate carbon source that enhances photosynthesis and growth of grape plantlets compared with the same concentrations of sucrose and glucose. The role of the sugars was regulated by the expression of key candidate genes related to hormones, key metabolic enzymes, and sugar metabolism of grape plantlets ( Vitis vinifera L.) grown in vitro. The addition of sugars including sucrose, glucose, and fructose is known to be very helpful for the development of grape (V...
May 26, 2017: Planta
https://www.readbyqxmd.com/read/28535465/altered-glycometabolism-in-zebrafish-exposed-to-thifluzamide
#8
Yang Yang, Wenxian Liu, Dongzhi Li, Le Qian, Bin Fu, Chengju Wang
Thifluzamide exerts toxic effects to zebrafish and causes liver mitochondrial damage. To better understand the further mechanism, adult zebrafish were exposed to a range of thifluzamide concentrations (0, 0.019, 0.19, and 1.90 mg/L) for 28 days. In response to 1.90 mg/L exposure, liver glycogen significantly increased and blood glucose decreased. The expression of genes related to glycometabolism showed corresponding changes. Genes related to mtDNA replication and transcription and genes participating in mitochondrial complexes showed altered expression, which might lead to the inhibition of the tricarboxylic acid cycle (TCA)...
May 10, 2017: Chemosphere
https://www.readbyqxmd.com/read/28529047/bezafibrate-prevents-mitochondrial-dysfunction-antioxidant-system-disturbance-glial-reactivity-and-neuronal-damage-induced-by-sulfite-administration-in-striatum-of-rats-implications-for-a-possible-therapeutic-strategy-for-sulfite-oxidase-deficiency
#9
Mateus Grings, Alana Pimentel Moura, Belisa Parmeggiani, Julia Tauana Pletsch, Gabriela Miranda Fernandez Cardoso, Pauline Maciel August, Cristiane Matté, Angela T S Wyse, Moacir Wajner, Guilhian Leipnitz
Sulfite accumulates in tissues of patients affected by sulfite oxidase (SO) deficiency, a neurometabolic disease characterized by seizures and progressive encephalopathy, often resulting in early death. We investigated the effects of sulfite on mitochondrial function, antioxidant system, glial reactivity and neuronal damage in rat striatum, as well as the potential protective effects of bezafibrate on sulfite-induced toxicity. Thirty-day-old rats were intrastriatally administered with sulfite (2μmol) or NaCl (2μmol; control) and euthanized 30min after injection for evaluation of biochemical parameters and western blotting, or 7days after injection for analysis of glial reactivity and neuronal damage...
May 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28516502/differential-effects-of-exposure-to-parasites-and-bacteria-on-stress-response-in-turbot-scophthalmus-maximus-simultaneously-stressed-by-low-water-depth
#10
J J Rodríguez-Quiroga, C Otero-Rodiño, P Suárez, T P Nieto, J M García Estévez, F San Juan, J L Soengas
The stress response of turbot Scophthalmus maximus was evaluated in fish maintained 8 days under different water depths, normal (NWD, 30 cm depth, total water volume 40 l) or low (LWD, 5 cm depth, total water volume 10 l), in the additional presence of infection-infestation of two pathogens of this species. This was caused by intraperitoneal injection of sublethal doses of the bacterium Aeromonas salmonicida subsp. salmonicida or the parasite Philasterides dicentrarchi (Ciliophora:Scuticociliatida)...
May 17, 2017: Journal of Fish Biology
https://www.readbyqxmd.com/read/28457947/influence-of-temperature-on-the-antioxidant-responses-and-lipid-peroxidation-of-two-species-of-tadpoles-rhinella-schneideri-and-physalaemus-nattereri-exposed-to-the-herbicide-sulfentrazone-boral-500sc%C3%A2
#11
Juliane Silberschmidt Freitas, Fabrício Barreto Teresa, Eduardo Alves de Almeida
Amphibians can experience large temperature fluctuations in their habitats, especially during the larval stage, when tadpoles are restricted to small and ephemeral ponds. Changes in water temperature can alter development, metabolism and behaviour of cold-blooded animals but also the toxicokinetics of chemicals in the environment. In Brazil, pesticides application is intensified during the rainy season, which is the period of reproduction for many amphibian species. We evaluated here the influence of temperature (28, 32, and 36°C) on the toxicity of the herbicide sulfentrazone (Boral®SC) in tadpoles of Physalaemus nattereri and Rhinella schneideri, by analysis of oxidative stress biomarkers...
April 27, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28429330/growth-kinetics-fatty-acid-composition-and-metabolic-activity-changes-of-crypthecodinium-cohnii-under-different-nitrogen-source-and-concentration
#12
Waseem Safdar, Muhammad Shamoon, Xinyi Zan, Junaid Haider, Hafiz Rizwan Sharif, Muhammad Shoaib, Yuanda Song
The effect of varying concentrations of the nitrogen source on the growth kinetics, lipid accumulation, lipid and DHA productivity, and fatty acid composition of C. cohnii was elucidated. Growth of C. cohnii was in three distinct growth stages: cell growth, lipid accumulation and a final lipid turnover stage. Most of lipids were accumulated in lipid accumulation stage (48-120 h) though, slow growth rate was observed during this stage. NaNO3 supported significantly higher lipid content (26.9% of DCW), DHA content (0...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28401536/autodisplay-of-glucose-6-phosphate-dehydrogenase-for-redox-cofactor-regeneration-at-the-cell-surface
#13
Jan Schüürmann, Paul Quehl, Fabian Lindhorst, Kristina Lang, Joachim Jose
Inherent cofactor regeneration is a pivotal feature of whole cell biocatalysis. For specific biotechnological applications, surface display of enzymes is emerging as a tool to circumvent mass transfer limitations or enzyme stability problems. Even complex reactions can be accomplished applying displayed enzymes. Yet, industrial utilization of the technique is still impeded by lacking cofactor regeneration at the cell surface. Here, we report on the surface display of a glucose-6-phoshate dehydrogenase (G6PDH) via Autodisplay to address this limitation and regenerate NADPH directly at the cell surface...
April 12, 2017: Biotechnology and Bioengineering
https://www.readbyqxmd.com/read/28343573/metabolic-and-cellular-stress-responses-of-catfish-horabagrus-brachysoma-g%C3%A3-nther-acclimated-to-increasing-temperatures
#14
Rishikesh S Dalvi, Tilak Das, Dipesh Debnath, Sona Yengkokpam, Kartik Baruah, Lalchand R Tiwari, Asim K Pal
We investigated the metabolic and cellular stress responses in an endemic catfish Horabagrus brachysoma acclimated to ambient (26°C), 31, 33 and 36°C for 30 days. After acclimation, fish were sampled to investigate changes in the levels of blood glucose, tissue glycogen and ascorbic acid, activities of enzymes involved in glycolysis (LDH), citric acid cycle (MDH), gluconeogenesis (FBPase and G6Pase), pentose phosphate pathway (G6PDH), protein metabolism (AST and ALT), phosphate metabolism (ACP and ALP) and energy metabolism (ATPase), and HSP70 levels in various tissues...
April 2017: Journal of Thermal Biology
https://www.readbyqxmd.com/read/28342961/particulate-metal-bioaccessibility-in-physiological-fluids-and-cell-culture-media-toxicological-perspectives
#15
Bérénice Leclercq, Laurent Yves Alleman, Esperanza Perdrix, Véronique Riffault, Mélanie Happillon, Alain Strecker, Jean-Marc Lo-Guidice, Guillaume Garçon, Patrice Coddeville
According to the literature, tiny amounts of transition metals in airborne fine particles (PM2.5) may induce proinflammatory cell response through reactive oxygen species production. The solubility of particle-bound metals in physiological fluids, i.e. the metal bioaccessibility is driven by factors such as the solution chemical composition, the contact time with the particles, and the solid-to-liquid phase ratio (S/L). In this work, PM2.5-bound metal bioaccessibility was assessed in various physiological-like solutions including cell culture media in order to evidence the potential impact on normal human bronchial epithelial cells (NHBE) when studying the cytotoxicity and inflammatory responses of PM2...
July 2017: Environmental Research
https://www.readbyqxmd.com/read/28336089/cold-lake-blend-diluted-bitumen-toxicity-to-the-early-development-of-japanese-medaka
#16
Barry N Madison, Peter V Hodson, Valerie S Langlois
Diluted bitumen (dilbit) from Alberta oil sands (Canada) is transported across major continental watersheds, yet little is known about its toxicity to fish if spilled into aquatic environments. The toxicity of Cold Lake (CLB) dilbit was assessed for medaka embryos (Oryzias latipes) exposed to water accommodated fractions (WAF) and chemically-enhanced WAF (CEWAF) using Corexit(®)EC9500A as dispersant. The effects of CLB toxicity were similar to conventional crude oils and Access Western Blend (AWB) dilbit. The prevalence of malformations and cyp1a mRNA synthesis in hatched fish increased monotonically with concentration during WAF and CEWAF treatments and provided a novel indicator of dilbit PAH toxicity...
June 2017: Environmental Pollution
https://www.readbyqxmd.com/read/28301876/autophagy-inhibitor-chloroquine-increases-sensitivity-to-cisplatin-in-qbc939-cholangiocarcinoma-cells-by-mitochondrial-ros
#17
Xianzhi Qu, Jiyao Sheng, Luyan Shen, Jing Su, Yunjie Xu, Qi Xie, Yao Wu, Xuewen Zhang, Liankun Sun
The tumor cells have some metabolic characteristics of the original tissues, and the metabolism of the tumor cells is closely related to autophagy. However, the mechanism of autophagy and metabolism in chemotherapeutic drug resistance is still poorly understood. In this study, we investigated the role and mechanism of autophagy and glucose metabolism in chemotherapeutic drug resistance by using cholangiocarcinoma QBC939 cells with primary cisplatin resistance and hepatocellular carcinoma HepG2 cells. We found that QBC939 cells with cisplatin resistance had a higher capacity for glucose uptake, consumption, and lactic acid generation, and higher activity of the pentose phosphate pathway compared with HepG2 cells, and the activity of PPP was further increased after cisplatin treatment in QBC939 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28260828/differential-site-based-expression-of-pentose-phosphate-pathway-related-proteins-among-breast-cancer-metastases
#18
Yoon Jin Cha, Woo Hee Jung, Ja Seung Koo
Purpose. We aimed to investigate the expression of pentose phosphate pathway- (PPP-) related proteins in metastatic breast cancer and its relationship with clinicopathologic factors. Methods. Tissue samples from 126 metastatic breast cancers were included in a tissue microarray. Expression of PPP-related proteins [glucose-6-phosphate dehydrogenase (G6PDH), 6-phosphogluconolactonase (6PGL), 6-phosphogluconate dehydrogenase (6PGDH), and nuclear factor erythroid 2-related factor (NRF2)] was determined by immunohistochemistry...
2017: Disease Markers
https://www.readbyqxmd.com/read/28251267/systematic-comparison-of-co-expression-of-multiple-recombinant-thermophilic-enzymes-in-escherichia-coli-bl21-de3
#19
Hui Chen, Rui Huang, Y-H Percival Zhang
The precise control of multiple heterologous enzyme expression levels in one Escherichia coli strain is important for cascade biocatalysis, metabolic engineering, synthetic biology, natural product synthesis, and studies of complexed proteins. We systematically investigated the co-expression of up to four thermophilic enzymes (i.e., α-glucan phosphorylase (αGP), phosphoglucomutase (PGM), glucose 6-phosphate dehydrogenase (G6PDH), and 6-phosphogluconate dehydrogenase (6PGDH)) in E. coli BL21(DE3) by adding T7 promoter or T7 terminator of each gene for multiple genes in tandem, changing gene alignment, and comparing one or two plasmid systems...
March 1, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28224472/effects-of-weight-reduction-after-sleeve-gastrectomy-on-metabolic-variables-in-saudi-obese-subjects-in-aseer-province-of-kingdom-of-saudi-arabia
#20
Mohammed A Bawahab, Abdullah S Assiri, Walid Abdel Maksoud, Ayyub Patel, Osama Kadoumi, Gaffar Sarwar Zaman, Riyad Mohammed Khalil Alessih, Syed Saleem Haider
OBJECTIVES: The objectives of this study were to investigate the occurrence of oxidative stress, status of protective antioxidants enzymes, inflammatory biomarkers, and some metabolic health variables in the blood and to compare the results between those of the normal controls and obese patients submitted to sleeve gastrectomy-induced weight loss over a 1-year follow-up period. MATERIALS AND METHODS: A prospective study was conducted in Aseer Central Hospital and Abha Private Hospital in the Kingdom of Saudi Arabia from January 2012 to January 2013 on 50 normal (BMI = 22-25 kg/m(2)) control subjects and 50 obese (BMI = 45-50 kg/m(2)) patients...
August 2017: Obesity Surgery
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