Katie A Mitzelfelt, Chris McDermott-Roe, Michael N Grzybowski, Maribel Marquez, Chieh-Ti Kuo, Michael Riedel, Shuping Lai, Melinda J Choi, Kurt D Kolander, Daniel Helbling, David P Dimmock, Michele A Battle, Chuanchau J Jou, Martin Tristani-Firouzi, James W Verbsky, Ivor J Benjamin, Aron M Geurts
Genome editing in induced pluripotent stem cells is currently hampered by the laborious and expensive nature of identifying homology-directed repair (HDR)-modified cells. We present an approach where isolation of cells bearing a selectable, HDR-mediated editing event at one locus enriches for HDR-mediated edits at additional loci. This strategy, called co-targeting with selection, improves the probability of isolating cells bearing HDR-mediated variants and accelerates the production of disease models.
February 22, 2017: Stem Cell Reports