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Sinorhizobium

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https://www.readbyqxmd.com/read/28990486/nodule-specific-cysteine-rich-peptides-negatively-regulate-nitrogen-fixing-symbiosis-in-a-strain-specific-manner-in-medicago-truncatula
#1
Qi Wang, Jinge Liu, Hua Li, Shengming Yang, Peter Körmöczi, Attila Kereszt, Hongyan Zhu
Medicago truncatula shows a high level of specificity when interacting with its symbiotic partner Sinorhizobium meliloti. This specificity is mainly manifested at the nitrogen-fixing stage of nodule development, such that a particular bacterial strain forms nitrogen-fixing nodules (Nod+/Fix+) on one plant genotype but ineffective nodules (Nod+/Fix-) on another. Recent studies have just begun to reveal the underlying molecular mechanisms that control this specificity. The S. meliloti strain A145 induces the formation of Fix+ nodules on the accession DZA315...
October 9, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28978203/silver-engineered-nanomaterials-and-ions-elicit-species-specific-o2-consumption-responses-in-plant-growth-promoting-rhizobacteria
#2
Ricky W Lewis, Jason Unrine, Paul M Bertsch, David H McNear
Metal containing engineered nanomaterials (ENMs) are now commonly used in various industrial and commercial applications. Many of these materials can be transformed during waste water treatment and ultimately enter terrestrial ecosystems via agriculturally applied biosolids. It is unclear how agriculturally important soil microbes will be affected by exposure to environmentally relevant, sublethal concentrations of ENMs and their transformation products (i.e., ions, aggregates, etc.). A method was developed, which puts O2 consumption responses in terms of viability, and tested by examining the toxic effects of Ag(+), Zn(2+), and Ni(2+) ions on the plant growth promoting rhizobacterium (PGPR) Bacillus amyloliquefaciens GB03...
October 4, 2017: Biointerphases
https://www.readbyqxmd.com/read/28967174/the-nitrate-reduction-gene-cluster-components-exert-lineage-dependent-contributions-to-optimization-of-sinorhizobium-symbiosis-with-soybeans
#3
Li Xue Liu, Qin Qin Li, Yun Zeng Zhang, Yue Hu, Jian Jiao, Hui Juan Guo, Xing Xing Zhang, Biliang Zhang, Wen Xin Chen, Chang Fu Tian
Receiving nodulation and nitrogen fixation genes does not guarantee rhizobia an effective symbiosis with legumes. Here, variations in gene content were determined for three Sinorhizobium species showing contrasting symbiotic efficiency on soybeans. A nitrate-reduction gene cluster absent in S. sojae was found to be essential for symbiotic adaptations of S. fredii and S. sp. III. In S. fredii, the deletion mutation of the nap (nitrate reductase), instead of nir (nitrite reductase) and nor (nitric oxide reductase), led to defects in nitrogen-fixation (Fix(-) )...
October 2, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28945538/gellertiella-hungarica-gen-nov-sp-nov-a-novel-bacterium-of-the-family-rhizobiaceae-isolated-from-a-spa-in-budapest
#4
Erika Tóth, Sára Szuróczki, Zsuzsa Kéki, Károly Bóka, Tibor Szili-Kovács, Peter Schumann
A novel alphaproteobacterium, strain RAM11T, belonging to the family Rhizobiaceae was isolated from the pool water of a thermal bath in Budapest, Hungary. Based on the 16S rRNA gene sequence strain RAM11T shows the highest sequence similarity values to Ensifer adhaerens Casida A (97.44 %), to Ensifer (syn. Sinorhizobium) americanus CFNEI 156T (96.87 %) and to Rhizobium azooxidifex Po 20/26T (96.76 %). The new bacterium is strictly aerobic, its optimum growth occurs at 20-37 °C, between pH 7 and 9 and without NaCl...
September 25, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28936222/the-soybean-rfg1-gene-restricts-nodulation-by-sinorhizobium-fredii-usda193
#5
Yinglun Fan, Jinge Liu, Shanhua Lyu, Qi Wang, Shengming Yang, Hongyan Zhu
Sinorhizobium fredii is a fast-growing rhizobial species that can establish a nitrogen-fixing symbiosis with a wide range of legume species including soybeans (Glycine max). In soybeans, this interaction shows a high level of specificity such that particular S. fredii strains nodulate only a limited set of plant genotypes. Here we report the identification of a dominant gene in soybeans that restricts nodulation with S. fredii USDA193. Genetic mapping in an F2 population revealed co-segregation of the underlying locus with the previously cloned Rfg1 gene...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28916561/succinate-transport-is-not-essential-for-symbiotic-nitrogen-fixation-by-sinorhizobium-meliloti-nor-rhizobium-leguminosarum
#6
Michael J Mitsch, George C diCenzo, Alison Cowie, Turlough M Finan
Symbiotic nitrogen fixation (SNF) is an energetically expensive process performed by bacteria during endosymbiotic relationships with plants. The bacteria require the plant to provide a carbon source for generation of reductant to power SNF. While C4-dicarboxylates (succinate, fumarate, malate) appear to be the primary, if not sole, carbon source provided to the bacteria, the contribution of each C4-dicarboxylate is not known. We address this issue using a genetic and systems-level analyses. Expression of a malate specific transporter (MaeP) in Sinorhizobium meliloti Rm1021 dct mutants unable to transport C4-dicarboxylates resulted in malate import rates up to 30% that of wild type...
September 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28912104/dissection-of-membrane-binding-and-remodeling-regions-in-two-classes-of-bacterial-phospholipid-n-methyltransferases
#7
Linna Danne, Meriyem Aktas, Nadine Grund, Tim Bentler, Ralf Erdmann, Franz Narberhaus
Bacterial phospholipid N-methyltransferases (Pmts) catalyze the formation of phosphatidylcholine (PC) via successive N-methylation of phosphatidylethanolamine (PE). They are classified into Sinorhizobium-type and Rhodobacter-type enzymes. The Sinorhizobium-type PmtA protein from the plant pathogen Agrobacterium tumefaciens is recruited to anionic lipids in the cytoplasmic membrane via two amphipathic helices called αA and αF. Besides its enzymatic activity, PmtA is able to remodel membranes mediated by the αA domain...
September 11, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28905086/identification-and-characterization-of-a-novel-vibo-quercitol-1-dehydrogenase-from-burkholderia-terrae-suitable-for-production-of-vibo-quercitol-from-2-deoxy-scyllo-inosose
#8
Nobuya Itoh, Junji Kurokawa, Hiroshi Toda, Kazunobu Konishi
(-)-vibo-Quercitol is a deoxyinositol (1L-1,2,4/3,5-cyclohexanepentol) that naturally occurs in oak species, honeydew honey, wines aged in oak barrels, and Gymnema sylvestre and is a potential intermediate for pharmaceuticals. We found that (-)-vibo-quercitol is stereoselectively synthesized from 2-deoxy-scyllo-inosose by the reductive reaction of a novel (-)-vibo-quercitol 1-dehydrogenase found in the proteomes of Burkholderia, Pseudomonas, and Arthrobacter. Among them, Burkholderia terrae sp. AKC-020 (40-1) produced a (-)-vibo-quercitol 1-dehydrogenase appropriate for synthesizing (-)-vibo-quercitol with a high diastereomeric excess...
September 13, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28905000/transcriptome-analysis-of-polyhydroxybutyrate-cycle-mutants-reveals-discrete-loci-connecting-nitrogen-utilization-and-carbon-storage-in-sinorhizobium-meliloti
#9
Maya D'Alessio, Ricardo Nordeste, Andrew C Doxey, Trevor C Charles
Polyhydroxybutyrate (PHB) and glycogen polymers are produced by bacteria as carbon storage compounds under unbalanced growth conditions. To gain insights into the transcriptional mechanisms controlling carbon storage in Sinorhizobium meliloti, we investigated the global transcriptomic response to the genetic disruption of key genes in PHB synthesis and degradation and in glycogen synthesis. Under both nitrogen-limited and balanced growth conditions, transcriptomic analysis was performed with genetic mutants deficient in PHB synthesis (phbA, phbB, phbAB, and phbC), PHB degradation (bdhA, phaZ, and acsA2), and glycogen synthesis (glgA1)...
September 2017: MSystems
https://www.readbyqxmd.com/read/28871850/succinoglycan-production-contributes-to-acidic-ph-tolerance-in-sinorhizobium-meliloti-rm1021
#10
Justin P Hawkins, Barney A Geddes, Ivan John Oresnik
In this work, the hypothesis that exopolysaccharide plays a role in the survival of S. meliloti at low pH is addressed. When S. meliloti was grown at pH 5.75 the synthesis of succinoglycan increased, whereas the synthesis of galactoglucan decreased. Succinoglycan that was isolated from cultures grown at low pH had a lower degree of polymerization relative to that which was isolated from cultures grown at neutral pH, suggesting that low molecular weight (LMW) succinoglycan might play a role in adaptation to low pH...
September 5, 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28867628/molecular-and-phenotypic-characterization-of-endophytic-bacteria-isolated-from-sulla-nodules
#11
Hamida Beghalem, Khadidja Aliliche, Ali Chriki, Ahmed Landoulsi
In the current study, bacterial diversity was investigated in root nodules of Sulla pallida and Sulla capitata. The isolates were analyzed on the basis of their phenotypic and molecular characteristics. The phylogenetic analysis based on 16S rRNA and housekeeping genes (recA and atpD) showed that the isolated bacteria related to Sinorhizobium, Neorhizobium, Phyllobacterium, Arthrobacter, Variovorax and Pseudomonas genera. This is the first report of Neorhizobium genus associated with Hedysarum genus. Phenotypically, all strains tolerate the elevated temperature of 40 °C, and salt stress at a concentration of 2%...
October 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28851745/a-key-regulator-of-the-glycolytic-and-gluconeogenic-central-metabolic-pathways-in-sinorhizobium-meliloti
#12
George C diCenzo, Zahed Muhammed, Magne Østerås, Shelley A P O'Brien, Turlough M Finan
The order Rhizobiales contains numerous agriculturally, biotechnologically, and medically important bacteria including the rhizobia, Agrobacterium, Brucella, and Methylobacterium, among others. These organisms tend to be metabolically versatile, but there has been relatively little investigation into the regulation of their central carbon metabolic pathways. Here, RNA-seq and promoter fusion data are presented to show that the PckR protein is a key regulator of central carbon metabolism in Sinorhizobium meliloti; during growth with gluconeogenic substrates, PckR represses expression of the complete Entner-Doudoroff glycolytic pathway and induces expression of the pckA and fbaB gluconeogenic genes...
August 29, 2017: Genetics
https://www.readbyqxmd.com/read/28842338/structure-proteome-and-genome-of-sinorhizobium-meliloti-phage-%C3%AE-m5-a-virus-with-luz24-like-morphology-and-a-highly-mosaic-genome
#13
Matthew C Johnson, Marta Sena-Velez, Brian K Washburn, Georgia N Platt, Stephen Lu, Tess E Brewer, Jason S Lynn, M Elizabeth Stroupe, Kathryn M Jones
Bacteriophages of nitrogen-fixing rhizobial bacteria are revealing a wealth of novel structures, diverse enzyme combinations and genomic features. Here we report the cryo-EM structure of the phage capsid at 4.9-5.7 Å-resolution, the phage particle proteome, and the genome of the Sinorhizobium meliloti-infecting Podovirus ΦM5. This is the first structure of a phage with a capsid and capsid-associated structural proteins related to those of the LUZ24-like viruses that infect Pseudomonas aeruginosa. Like many other Podoviruses, ΦM5 is a T=7 icosahedron with a smooth capsid and short, relatively featureless tail...
August 22, 2017: Journal of Structural Biology
https://www.readbyqxmd.com/read/28827694/a-novel-c-di-gmp-binding-domain-in-glycosyltransferase-bgsa-is-responsible-for-the-synthesis-of-a-mixed-linkage-%C3%AE-glucan
#14
Daniel Pérez-Mendoza, Daniela Bertinetti, Robin Lorenz, María-Trinidad Gallegos, Friedrich W Herberg, Juan Sanjuán
BgsA is the glycosyltransferase (GT) involved in the synthesis of a linear mixed-linkage β-glucan (MLG), a recently described exopolysaccharide activated by c-di-GMP in Sinorhizobium meliloti and other Rhizobiales. Although BgsA displays sequence and structural homology with bacterial cellulose synthases (CS), it does not contain any predictable c-di-GMP binding domain. In this work we demonstrate that the cytoplasmic C-terminal domain of BgsA (C-BgsA) binds c-di-GMP with both high affinity (KD = 0.23 μM) and specificity...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28805544/rna-silencing-in-plant-symbiotic-bacteria-insights-from-a-protein-centric-view
#15
José I Jiménez-Zurdo, Marta Robledo
Extensive work in model enterobacteria has evidenced that the RNA chaperone Hfq and several endoribonucleases, such as RNase E or RNase III, serve pivotal roles in small RNA-mediated post-transcriptional silencing of gene expression. Characterization of these protein hubs commonly provide global functional and mechanistic insights into complex sRNA regulatory networks. The legume endosymbiont Sinorhizobium meliloti is a non-classical model bacterium with a very complex lifestyle in which riboregulation is expected to play important adaptive functions...
August 14, 2017: RNA Biology
https://www.readbyqxmd.com/read/28800133/adaptive-evolution-of-rhizobial-symbiotic-compatibility-mediated-by-co-evolved-insertion-sequences
#16
Ran Zhao, Li Xue Liu, Yun Zeng Zhang, Jian Jiao, Wen Jing Cui, Biliang Zhang, Xiao Lin Wang, Meng Lin Li, Yi Chen, Zhu Qing Xiong, Wen Xin Chen, Chang Fu Tian
Mutualism between bacteria and eukaryotes has essential roles in the history of life, but the evolution of their compatibility is poorly understood. Here we show that different Sinorhizobium strains can form either nitrogen-fixing nodules or uninfected pseudonodules on certain cultivated soybeans, while being all effective microsymbionts of some wild soybeans. However, a few well-infected nodules can be found on a commercial soybean using inocula containing a mixed pool of Tn5 insertion mutants derived from an incompatible strain...
August 11, 2017: ISME Journal
https://www.readbyqxmd.com/read/28768212/effects-of-biosolids-from-a-wastewater-treatment-plant-receiving-manufactured-nanomaterials-on-medicago-truncatula-and-associated-soil-microbial-communities-at-low-nanomaterial-concentrations
#17
Chun Chen, Olga V Tsyusko, Dave H McNear, Jonathan Judy, Ricky W Lewis, Jason M Unrine
Concern has grown regarding engineered nanomaterials (ENMs) entering agricultural soils through the application of biosolids and their possible effects on agroecosystems, even though the ENMs are extensively transformed. The effects of exposure to biosolids containing transformation products of these ENMs at low concentrations remain largely unexplored. We examined the responses of Medicago truncatula and its symbiotic rhizobia Sinorhizobium meliloti exposed to soil amended with biosolids from WWTP containing low added concentrations of ENMs (ENM Low), bulk/dissolved metals (bulk/dissolved Low), or no metal additions (control)...
December 31, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28765224/genome-wide-sensitivity-analysis-of-the-microsymbiont-sinorhizobium-meliloti-to-symbiotically-important-defensin-like-host-peptides
#18
Markus F F Arnold, Mohammed Shabab, Jon Penterman, Kevin L Boehme, Joel S Griffitts, Graham C Walker
The model legume species Medicago truncatula expresses more than 700 nodule-specific cysteine-rich (NCR) signaling peptides that mediate the differentiation of Sinorhizobium meliloti bacteria into nitrogen-fixing bacteroids. NCR peptides are essential for a successful symbiosis in legume plants of the inverted-repeat-lacking clade (IRLC) and show similarity to mammalian defensins. In addition to signaling functions, many NCR peptides exhibit antimicrobial activity in vitro and in vivo Bacterial resistance to these antimicrobial activities is likely to be important for symbiosis...
August 1, 2017: MBio
https://www.readbyqxmd.com/read/28756029/tripartite-symbiosis-of-sophora-tomentosa-rhizobia-and-arbuscular-mycorhizal-fungi
#19
Maíra Akemi Toma, Teotonio Soares de Carvalho, Amanda Azarias Guimarães, Elaine Martins da Costa, Jacqueline Savana da Silva, Fatima Maria de Souza Moreira
Sophora tomentosa is a pantropical legume species with potential for recovery of areas degraded by salinization, and for stabilization of sand dunes. However, few studies on this species have been carried out, and none regarding its symbiotic relationship with beneficial soil microorganisms. Therefore, this study aimed to evaluate the diversity of nitrogen-fixing bacteria isolated from nodules of Sophora tomentosa, and to analyze the occurrence of colonization of arbuscular mycorrhizal fungi on the roots of this legume in seafront soil...
October 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28745538/sinorhizobium-meliloti-chemotaxis-to-multiple-amino-acids-is-mediated-by-the-chemoreceptor-mcpu
#20
Benjamin A Webb, K Karl Compton, Julia S Martin Del Campo, Doris Taylor, Pablo Sobrado, Birgit E Scharf
The legume symbiont Sinorhizobium meliloti is chemoattracted to compounds exuded by germinating seeds of its host alfalfa. This response is mainly mediated by the S. meliloti chemoreceptor McpU. McpU also has a prominent contribution in sensing a synthetic amino acid (aa) mixture mimicking the amounts and composition observed in seed exudate. Here, we used the hydrogel capillary assay to quantify chemotactic responses of S. meliloti to individual aa exuded by germinating alfalfa seeds and to define the role of McpU in this behavior...
July 26, 2017: Molecular Plant-microbe Interactions: MPMI
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