keyword
MENU ▼
Read by QxMD icon Read
search

Sinorhizobium

keyword
https://www.readbyqxmd.com/read/28314132/rhizobial-symbiosis-alleviates-polychlorinated-biphenyls-induced-systematic-oxidative-stress-via-brassinosteroids-signaling-in-alfalfa
#1
Xiaomi Wang, Ying Teng, Ning Zhang, Peter Christie, Zhengao Li, Yongming Luo, Jun Wang
The role of symbiotic rhizobia in the alleviation of polychlorinated biphenyl (PCB)-induced phytotoxicity in alfalfa and the brassinosteroid (BR) hormone signaling involved were investigated during phytoremediation. The association between alfalfa and Sinorhizobium meliloti was adopted as a remediation model. Phytotoxicity due to PCB 77 (3,3',4,4'-tetrachlorobiphenyl) exerted adverse impacts on plant performance (biomass accumulation and photosynthesis) and elicited cellular oxidative stress (overproduction of reactive oxygen species, lipid peroxidation, and cell necrosis) which was largely attenuated by pre-inoculation with S...
March 14, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28285657/two-component-regulatory-system-acts-actr-is-required-for-sinorhizobium-meliloti-adaptation-to-oxidative-stress
#2
Guirong Tang, Sunjun Wang, Dawei Lu, Leqi Huang, Ningning Li, Li Luo
The two-component system ActS/ActR plays important roles in bacterial adaptation to abiotic stress, including acid tolerance and oxidant resistance. However, the underlying regulatory mechanism is not clear. In this study, we found that the ActS/ActR system is required for adaptation to oxidative stress by regulating the transcription of the genes actR, katB, gshA and gshB1. The actS and actR mutants were sensitive to low pH and oxidants such as H2O2, oxidized glutathione (GSSG) and sodium nitroprusside (SNP)...
May 2017: Microbiological Research
https://www.readbyqxmd.com/read/28284052/high-yield-production-of-rhizobium-nodb-chitin-deacetylase-and-its-use-for-in%C3%A2-vitro-synthesis-of-lipo-chitinoligosaccharide-precursors
#3
Rémi Chambon, Stéphanie Pradeau, Sébastien Fort, Sylvain Cottaz, Sylvie Armand
Lipo-chitinoligosaccharides (LCOs) are key molecules for the establishment of plant-microorganisms symbiosis. Interactions of leguminous crops with nitrogen-fixing rhizobial bacteria involve Nod factors, while Myc-LCOs improve the association of most plants with arbuscular mycorrhizal fungi. Both Nod factors and Myc-LCOs are composed of a chitinoligosaccharide fatty acylated at the non-reducing end accompanied with various substituting groups. One straightforward way to access LCOs is starting from chitin hydrolysate, an abundant polysaccharide found in crustacean shells, followed by regioselective enzymatic cleavage of an acetyl group from the non-reducing end of chitin tetra- or pentaose, and subsequent chemical introduction of N-acyl group...
February 27, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28273103/functional-metagenomics-reveals-novel-%C3%AE-galactosidases-not-predictable-from-gene-sequences
#4
Jiujun Cheng, Tatyana Romantsov, Katja Engel, Andrew C Doxey, David R Rose, Josh D Neufeld, Trevor C Charles
The techniques of metagenomics have allowed researchers to access the genomic potential of uncultivated microbes, but there remain significant barriers to determination of gene function based on DNA sequence alone. Functional metagenomics, in which DNA is cloned and expressed in surrogate hosts, can overcome these barriers, and make important contributions to the discovery of novel enzymes. In this study, a soil metagenomic library carried in an IncP cosmid was used for functional complementation for β-galactosidase activity in both Sinorhizobium meliloti (α-Proteobacteria) and Escherichia coli (γ-Proteobacteria) backgrounds...
2017: PloS One
https://www.readbyqxmd.com/read/28264559/a-family-of-single-copy-repabc-type-shuttle-vectors-stably-maintained-in-the-alpha-proteobacterium-sinorhizobium-meliloti
#5
Johannes Döhlemann, Marcel Wagner, Carina Happel, Martina Carrillo, Patrick Sobetzko, Tobias J Erb, Martin Thanbichler, Anke Becker
A considerable share of bacterial species maintains segmented genomes. Plant symbiotic α-proteobacterial rhizobia contain up to six repABC-type replicons in addition to the primary chromosome. These low or unit-copy replicons, classified as secondary chromosomes, chromids, or megaplasmids, are exclusively found in α-proteobacteria. Replication and faithful partitioning of these replicons to the daughter cells is mediated by the repABC region. The importance of α-rhizobial symbiotic nitrogen fixation for sustainable agriculture and Agrobacterium-mediated plant transformation as a tool in plant sciences has increasingly moved biological engineering of these organisms into focus...
March 20, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28253454/common-dyes-used-to-determine-bacterial-polysaccharides-on-agar-are-affected-by-medium-acidification
#6
Justin P Hawkins, Barney A Geddes, Ivan John Oresnik
In this work the effects of pH on bacterial phenotypes when using the bacteriological dyes Aniline blue, Congo red, and Calcofluor white to analyze polysaccharide production. Study of galactose catabolism in <i>Sinorhizobium melilot</i>i led to the isolation of a mutation in <i>dgok1</i>, which was observed to overproduce exopolysaccharides when grown in the presence of galactose. When this strain was spotted onto plates containing Aniline blue, Congo red, or Calcofluor white, the intensity of the associated staining was strikingly different when compared to the Wild-type...
March 2, 2017: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/28235609/massive-parallel-insertion-site-sequencing-of-an-arrayed-sinorhizobium-meliloti-signature-tagged-mini-tn-5-transposon-mutant-library
#7
Javier Serrania, Tobias Johner, Oliver Rupp, Alexander Goesmann, Anke Becker
Transposon mutagenesis in conjunction with identification of genomic transposon insertion sites is a powerful tool for gene function studies. We have implemented a protocol for parallel determination of transposon insertion sites by Illumina sequencing involving a hierarchical barcoding method that allowed for tracking back insertion sites to individual clones of an arrayed signature-tagged transposon mutant library. This protocol was applied to further characterize a signature-tagged mini-Tn 5 mutant library comprising about 12,000 mutants of the symbiotic nitrogen-fixing alphaproteobacterium Sinorhizobium meliloti (Pobigaylo et al...
February 21, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28217917/a-comprehensive-phylogenetic-analysis-of-copper-transporting-p1b-atpases-from-bacteria-of-the-rhizobiales-order-uncovers-multiplicity-diversity-and-novel-taxonomic-subtypes
#8
Ciro Cubillas, Fabiola Miranda-Sánchez, Antonio González-Sánchez, José Pedro Elizalde, Pablo Vinuesa, Susana Brom, Alejandro García-de Los Santos
The ubiquitous cytoplasmic membrane copper transporting P1B-1 and P1B-3 -type ATPases pump out Cu(+) and Cu(2+) , respectively, to prevent cytoplasmic accumulation and avoid toxicity. The presence of five copies of Cu-ATPases in the symbiotic nitrogen-fixing bacteria Sinorhizobium meliloti is remarkable; it is the largest number of Cu(+) -transporters in a bacterial genome reported to date. Since the prevalence of multiple Cu-ATPases in members of the Rhizobiales order is unknown, we performed an in silico analysis to understand the occurrence, diversity and evolution of Cu(+) -ATPases in members of the Rhizobiales order...
February 20, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28216096/alkalinity-of-lanzarote-soils-is-a-factor-shaping-rhizobial-populations-with-sinorhizobium-meliloti-being-the-predominant-microsymbiont-of-lotus-lancerottensis
#9
Milagros León-Barrios, Juan Pérez-Yépez, Paola Dorta, Ana Garrido, Concepción Jiménez
Lotus lancerottensis is an endemic species that grows widely throughout Lanzarote Island (Canary Is.). Characterization of 48 strains isolated from root nodules of plants growing in soils from eleven locations on the island showed that 38 isolates (79.1%) belonged to the species Sinorhizobium meliloti, whereas only six belonged to Mesorhizobium sp., the more common microsymbionts for the Lotus. Other genotypes containing only one isolate were classified as Pararhizobium sp., Sinorhizobium sp., Phyllobacterium sp...
February 2, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28197647/the-overproduction-of-indole-3-acetic-acid-iaa-in-endophytes-upregulates-nitrogen-fixation-in-both-bacterial-cultures-and-inoculated-rice-plants
#10
Roberto Defez, Anna Andreozzi, Carmen Bianco
Endophytic bacteria from roots and leaves of rice plants were isolated and identified in order to select the diazotrophs and improve their nitrogen-fixing abilities. The nitrogen-fixing endophytes were identified by PCR amplification of the nifH gene fragment. For this purpose, two isolates, Enterobacter cloacae RCA25 and Klebsiella variicola RCA26, and two model bacteria (Herbaspirillum seropedicae z67 and Sinorhizobium fredii NGR234) were transformed to increase the biosynthesis of the main plant auxin indole-3-acetic acid (IAA)...
February 14, 2017: Microbial Ecology
https://www.readbyqxmd.com/read/28194158/role-and-regulation-of-acc-deaminase-gene-in-sinorhizobium-meliloti-is-it-a-symbiotic-rhizospheric-or-endophytic-gene
#11
Alice Checcucci, Elisa Azzarello, Marco Bazzicalupo, Anna De Carlo, Giovanni Emiliani, Stefano Mancuso, Giulia Spini, Carlo Viti, Alessio Mengoni
Plant-associated bacteria exhibit a number of different strategies and specific genes allow bacteria to communicate and metabolically interact with plant tissues. Among the genes found in the genomes of plant-associated bacteria, the gene encoding the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase (acdS) is one of the most diffused. This gene is supposed to be involved in the cleaving of plant-produced ACC, the precursor of the plant stress-hormone ethylene toning down the plant response to infection...
2017: Frontiers in Genetics
https://www.readbyqxmd.com/read/28180335/sinorhizobium-meliloti-ybey-is-an-endoribonuclease-with-unprecedented-catalytic-features-acting-as-silencing-enzyme-in-riboregulation
#12
Margarida Saramago, Alexandra Peregrina, Marta Robledo, Rute G Matos, Rolf Hilker, Javier Serrania, Anke Becker, Cecilia M Arraiano, José I Jiménez-Zurdo
No abstract text is available yet for this article.
February 17, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/28167519/regulation-of-polyhydroxybutyrate-accumulation-in-sinorhizobium-meliloti-by-the-trans-encoded-small-rna-mmgr
#13
Antonio Lagares, Germán Ceizel Borella, Uwe Linne, Anke Becker, Claudio Valverde
Riboregulation has a major role in the fine-tuning of multiple bacterial processes. Among the RNA players, trans-encoded untranslated small RNAs (sRNAs) regulate complex metabolic networks by tuning expression from multiple target genes in response to numerous signals. In Sinorhizobium meliloti, over 400 sRNAs are expressed under different stimuli. The sRNA MmgR-standing for Makes more granules Regulator-has been of particular interest to us since its sequence and structure are highly conserved among the α-proteobacteria, and its expression is regulated by the amount and quality of the bacterium's available nitrogen source...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28141492/rnase-e-and-rnase-j-are-needed-for-s-adenosylmethionine-homeostasis-in-sinorhizobium-meliloti
#14
Kathrin Baumgardt, Hendrik Melior, Ramakanth Madhugiri, Sebastian Thalmann, Adam Schikora, Matthew McIntosh, Anke Becker, Elena Evguenieva-Hackenberg
The ribonucleases (RNases) E and J play major roles in Escherichia coli and Bacillus subtilis, respectively, and co-exist in Sinorhizobium meliloti. We analyzed S. meliloti 2011 mutants with mini-Tn5 insertions in the corresponding genes rne and rnj and found many overlapping effects. We observed similar changes in mRNA levels including lower mRNA levels of the motility and chemotaxis related genes flaA, flgB, and cheR and higher levels of ndvA (important for glucan export). The acyl-homoserine lactone (AHL) levels were also higher during exponential growth in both RNase mutants, despite no increase in the expression of the sinI AHL synthase gene...
January 28, 2017: Microbiology
https://www.readbyqxmd.com/read/28138345/genomic-characterization-of-eight-ensifer-strains-isolated-from-pristine-caves-and-a-whole-genome-phylogeny-of-ensifer-sinorhizobium
#15
Heerman Kumar Sandra Kumar, Han Ming Gan, Mun Hua Tan, Wilhelm Wei Han Eng, Hazel A Barton, André O Hudson, Michael A Savka
A total of eight Ensifer sp. strains were isolated from two pristine cave environments. One strain was isolated from a cave water pool located in the Wind Cave National Park, South Dakota, USA and the remaining seven strains were isolated from Lechuguilla Cave of Carlsbad Caverns National Park, New Mexico, USA. Whole genome sequencing and comparative genomic analyses of the eight isolates compared to various type strains from the genera Ensifer and Sinorhizobium demonstrates that although members in these genera can be phylogenetically separated into two distinct clades, the percentage of conserved proteins (POCP) between various type strains from Ensifer and Sinorhizobium are consistently higher than 50%, providing strong genomic evidence to support the classification of the genera Ensifer and Sinorhizobium into a single genus...
2017: Journal of Genomics
https://www.readbyqxmd.com/read/28088165/genomic-characterization-of-ensifer-aridi-a-proposed-new-species-of-nitrogen-fixing-rhizobium-recovered-from-asian-african-and-american-deserts
#16
Antoine Le Quéré, Nisha Tak, Hukam Singh Gehlot, Celine Lavire, Thibault Meyer, David Chapulliot, Sonam Rathi, Ilham Sakrouhi, Guadalupe Rocha, Marine Rohmer, Dany Severac, Abdelkarim Filali-Maltouf, Jose-Antonio Munive
BACKGROUND: Nitrogen fixing bacteria isolated from hot arid areas in Asia, Africa and America but from diverse leguminous plants have been recently identified as belonging to a possible new species of Ensifer (Sinorhizobium). In this study, 6 strains belonging to this new clade were compared with Ensifer species at the genome-wide level. Their capacities to utilize various carbon sources and to establish a symbiotic interaction with several leguminous plants were examined. RESULTS: Draft genomes of selected strains isolated from Morocco (Merzouga desert), Mexico (Baja California) as well as from India (Thar desert) were produced...
January 14, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28058638/functional-analysis-of-the-two-cyclophilin-isoforms-of-sinorhizobium-meliloti
#17
Eirini-Evangelia Thomloudi, Aggeliki Skagia, Anastasia Venieraki, Panagiotis Katinakis, Maria Dimou
The nitrogen fixing Sinorhizobium meliloti possesses two genes, ppiA and ppiB, encoding two cyclophilin isoforms which belong to the superfamily of peptidyl prolyl cis/trans isomerases (PPIase, EC: 5.2.1.8). Here, we functionally characterize the two proteins and we demonstrate that both recombinant cyclophilins are able to isomerise the Suc-AAPF-pNA synthetic peptide but neither of them displays chaperone function in the citrate synthase thermal aggregation assay. Furthermore, we observe that the expression of both enzymes increases the viability of E...
February 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28017611/regulation-of-differentiation-of-nitrogen-fixing-bacteria-by-microsymbiont-targeting-of-plant-thioredoxin-s1
#18
Carolina Werner Ribeiro, Fabien Baldacci-Cresp, Olivier Pierre, Marie Larousse, Sofiane Benyamina, Annie Lambert, Julie Hopkins, Claude Castella, Julie Cazareth, Geneviève Alloing, Eric Boncompagni, Jérémy Couturier, Peter Mergaert, Pascal Gamas, Nicolas Rouhier, Françoise Montrichard, Pierre Frendo
Legumes associate with rhizobia to form nitrogen (N2)-fixing nodules, which is important for plant fitness [1, 2]. Medicago truncatula controls the terminal differentiation of Sinorhizobium meliloti into N2-fixing bacteroids by producing defensin-like nodule-specific cysteine-rich peptides (NCRs) [3, 4]. The redox state of NCRs influences some biological activities in free-living bacteria, but the relevance of redox regulation of NCRs in planta is unknown [5, 6], although redox regulation plays a crucial role in symbiotic nitrogen fixation [7, 8]...
January 23, 2017: Current Biology: CB
https://www.readbyqxmd.com/read/28007516/using-transposition-to-introduce-egfp-fusions-in-sinorhizobium-meliloti-a-tool-to-analyze-protein-localization-patterns-in-bacteria
#19
Hanna Bednarz, Karsten Niehaus
Conventional methods used for the in vivo analysis of subcellular protein localizations and their spatio-temporal dynamics in prokaryotes are based on either the engineering of N(amino)- or C(carboxy)-terminal fusions of fluorescent proteins with the protein of interest, or involved probing internal sites for tag integration. In addition, the use of inducible or constitutive promoters for the expression of fluorescent fusion proteins can lead to overexpression and result in localization artifacts. Here, we describe a method for the synthesis of fluorescent fusion proteins using transposable elements, which can randomly integrate in the internal sections of the protein coding sequence to produce full-length fluorescent fusion proteins expressed at endogenous levels...
December 19, 2016: Journal of Biotechnology
https://www.readbyqxmd.com/read/27986730/the-sinorhizobium-ensifer-fredii-hh103-nodulation-outer-protein-nopi-is-a-determinant-for-efficient-nodulation-of-soybean-and-cowpea-plants
#20
Irene Jiménez-Guerrero, Francisco Pérez-Montaño, Carlos Medina, Francisco Javier Ollero, Francisco Javier López-Baena
The type III secretion system (T3SS) is a specialized secretion apparatus that is commonly used by many plant and animal pathogenic bacteria to deliver proteins, termed effectors, to the interior of the host cells. These effectors suppress host defenses and interfere with signal transduction pathways to promote infection. Some rhizobial strains possess a functional T3SS, which is involved in the suppression of host defense responses, host range determination, and symbiotic efficiency. The analysis of the genome of the broad-host-range rhizobial strain Sinorhizobium fredii HH103 identified eight genes that code for putative T3SS effectors...
March 1, 2017: Applied and Environmental Microbiology
keyword
keyword
19550
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"