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Laura Rubio-Sanz, Belén Brito, Jose Palacios
Natural habitats containing high amounts of heavy metals provide a valuable source of bacteria adapted to deal with metal toxicity. A functional analysis of the population of legume endosymbiotic bacteria in an ultramafic soil was undertaken by studying a collection of Rhizobium leguminosarum bv viciae (Rlv) isolates obtained using pea as trap plant. One of the isolates, Rlv UPM1137, was selected on the basis of its higher tolerance to nickel and cobalt and presence of inducible mechanisms for such tolerance...
January 17, 2018: FEMS Microbiology Letters
Antoine Berger, Renaud Brouquisse, Pradeep Kumar Pathak, Imène Hichri, Inderjit Singh, Sabhyata Bhatia, Alexandre Boscari, Abir U Igamberdiev, Kapuganti Jagadis Gupta
The interaction between legumes and rhizobia leads to the establishment of a beneficial symbiotic relationship. Recent advances in legume - rhizobium symbiosis revealed that various reactive oxygen and nitrogen species including nitric oxide (NO) play important roles during this process. Nodule development occurs with a transition from a normoxic environment during the establishment of symbiosis to a microoxic environment in functional nodules. Such oxygen dynamics are required for activation and repression of various NO production and scavenging pathways...
January 19, 2018: Plant, Cell & Environment
Christopher M Elvin, Robert E Asenstorfer, Maarten H Ryder, Scott C Donner, Graham P Jones, Max E Tate
Agrocin 108 is a 3'-O-β-D-xylopyranosyl-cytidine-5'-O-phosphodiester of an ascorbate-carbocyclic cyclopentenone analogue, with bacteriocin-like properties. This bacteriocin exhibits orders of magnitude greater than the inhibition zone diameter towards the indicator strain than either ampicillin or streptomycin. It has been isolated from cultures of Rhizobium rhizogenes strain K108. The structure of the agrocin 108 without detail, has been previously published. We now report a detailed structure elucidation, including the hitherto undetermined residual 5'-phospho-diester fragment by a combination of 1D and 2D NMR studies at various pH values in H2O/D2O, high resolution MS, pKa determination, and chemical degradation...
January 18, 2018: Journal of Antibiotics
Marta Robledo, Esther Menéndez, Jose Ignacio Jimenez-Zurdo, Raúl Rivas, Encarna Velazquez, Eustoquio Martinez-Molina, Giles Oldroyd, Pedro F Mateos
The infection of legume plants by rhizobia is tightly regulated to ensure accurate bacterial penetration, infection and development of functionally efficient nitrogen-fixing root nodules. Rhizobial Nod Factors (NF) have key roles in the elicitation of nodulation signalling. Infection of white clover roots also involves the tightly regulated specific breakdown of the non-crystalline apex of cell walls in growing root hairs, which is mediated by Rhizobium leguminosarum bv. trifolii cellulase CelC2. Here, we have analyzed the impact of this endoglucanase on symbiotic signalling in the model legume Medicago truncatula...
January 15, 2018: Molecular Plant-microbe Interactions: MPMI
Maria J Tavares, Francisco X Nascimento, Bernard R Glick, Márcio J Rossi
Ethylene acts as an inhibitor of the nodulation process of leguminous plants. However, some bacteria can decrease deleterious ethylene levels by the action of the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase which degrades ACC, the ethylene precursor in all higher plants. Co-inoculation of rhizobia with endophytes enhances the rhizobial symbiotic efficiency with legumes, improving both nodulation and nitrogen fixation. However, not much is understood about the mechanisms employed by these endophytic bacteria...
January 11, 2018: Letters in Applied Microbiology
Hanna Rose, Edgar Maiss
The complete genome sequence of a German isolate of celery mosaic virus (CeMV, a potyvirus) from Quedlinburg (DSMZ PV-1003) was determined (MF962880). This represents the second fully sequenced genome of this virus, along with a Californian isolate (HQ676607.1). The positive-sense single-stranded RNA is 10,000 nucleotides in length and shows the typical organization of potyviruses but has a shorter PIPO than CeMV California. In comparison to CeMV isolates from different origins, CeMV-Quedlinburg and isolates from the Netherlands (AF203531...
January 11, 2018: Archives of Virology
Alejandro Jiménez-Gómez, José David Flores-Félix, Paula García-Fraile, Pedro F Mateos, Esther Menéndez, Encarna Velázquez, Raúl Rivas
The growing interest in a healthy lifestyle and in environmental protection is changing habits regarding food consumption and agricultural practices. Good agricultural practice is indispensable, particularly for raw vegetables, and can include the use of plant probiotic bacteria for the purpose of biofertilization. In this work we analysed the probiotic potential of the rhizobial strain PEPV40, identified as Rhizobium laguerreae through the analysis of the recA and atpD genes, on the growth of spinach plants...
January 10, 2018: Scientific Reports
Chunjie Liang, Ting He, Tao Li, Yunhuang Yang, Jiang Zhu, Maili Liu
AHSA1 protein family is one of the four largest families in the Bet v1-like protein superfamily. The functions and structures of proteins in AHSA1 family are still largely unknown. CHU_1110 with 167 amino acids and a molecular weight of 19.2 kDa is a member of the AHSA1 family from Cytophaga hutchinsonii, a soil bacterium known for its ability to digest crystalline cellulose. Here we report the complete 1H, 13C and 15N chemical shift assignments of CHU_1110. The secondary structural elements of CGL2373 are consistent with the canonical AHSA1 structure...
January 9, 2018: Biomolecular NMR Assignments
Djellali Belhadi, Philippe de Lajudie, Nacer Ramdani, Christine Le Roux, Farida Boulila, Pierre Tisseyre, Abdelghani Boulila, Amar Benguedouar, Yahia Kaci, Gisèle Laguerre
Fifty-eight rhizobial strains were isolated from root nodules of Vicia faba cv. Equina and Vicia faba cv. Minor by the host-trapping method in soils collected from eleven sites in Bejaia, Eastern Algeria. Eleven genotypic groups were distinguished based on the combined PCR/RFLP of 16S rRNA, 16S-23S rRNA intergenic spacer and symbiotic (nodC and nodD-F) genes and further confirmed by multilocus sequence analysis (MLSA) of three housekeeping genes (recA, atpD and rpoB), the 16S rRNA gene and the nodulation genes nodC and nodD...
December 27, 2017: Systematic and Applied Microbiology
Kaoutar Taha, El Bekkay Berraho, Imane El Attar, Samia Dekkiche, Jamal Aurag, Gilles Béna
Genetic diversity and population structure of 268 Lens culinaris symbiotic rhizobia collected from 40 cultivated fields in the main lentil production regions in Morocco were estimated. Three chromosomal housekeeping genes (recA, glnII and atpD) and one common symbiotic gene (nodC) were sequenced and analyzed in order to identify the local symbionts of lentil. The molecular phylogeny of the concatenated housekeeping genes clustered more than 95% of the isolates in one main clade together with Rhizobium laguerreae species...
December 21, 2017: Systematic and Applied Microbiology
Manoj-Kumar Arthikala, Kalpana Nanjareddy, Miguel Lara
BYPASS1 (BPS1), which is a well-conserved gene in plants, is required for normal root and shoot development. In the absence of BPS1 gene function, Arabidopsis overproduces a mobile signalling compound (the BPS1 signal) in roots, and this transmissible signal arrests shoot growth and causes abnormal root development. In addition to the shoot and root meristem activities, the legumes also possess transient meristematic activity in root cortical cells during Rhizobium symbiosis. We explored the role of Phaseolus vulgaris BPS1 during nodule primordium development using an RNA-interference (RNAi) silencing approach...
January 3, 2018: Genes
Eunae Cho, Seunho Jung
The poor water solubility of paclitaxel causes significant problems in producing cancer therapeutic formulations. Here, we aimed to solubilize paclitaxel using biocompatible cyclic carbohydrates. Generally recognized as safe, labeled β-cyclodextrin (β-CD), a cyclic α-1,4-glucan consisting of seven glucoses, was prepared, and bio-sourced cyclosophoraoses (CyS), which are unbranched cyclic β-1,2-glucans with 17-23 glucose units, were purified using various chromatographic methods from Rhizobium leguminosarum cultural broth...
January 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Paulo Cardoso, Sofia Corticeiro, Rosa Freitas, Etelvina Figueira
Soil contamination with metals is a widespread problem posing risks to humans and ecosystems. Metal contaminated soils often hold poor microbial density and biodiversity. Among soil bacteria, rhizobia have a great agronomic and environmental significance and are major contributors to a sustainable maintenance of soil fertility. This group of microorganisms are severely affected by metals, such as cadmium (Cd), but information about metal resistance mechanisms in rhizobia is still limited. A concerted approach of the different mechanisms conferring Cd tolerance to rhizobia was conducted using two Rhizobium strains with contrasting tolerances to Cd...
December 28, 2017: Ecotoxicology and Environmental Safety
Marta Robledo, Natalia I García-Tomsig, José I Jiménez-Zurdo
High-throughput transcriptome profiling (RNAseq) has uncovered large and heterogeneous populations of small noncoding RNA species (sRNAs) with potential regulatory roles in bacteria. A large fraction of sRNAs are differentially regulated and rely on protein-assisted antisense interactions to trans-encoded target mRNAs to fine-tune posttranscriptional reprogramming of gene expression in response to external cues. However, annotation and function of sRNAs are still largely overlooked in nonmodel bacteria with complex lifestyles...
2018: Methods in Molecular Biology
Agnieszka Golicz, Mohan B Singh, Prem L Bhalla
Long intergenic non-coding RNAs (lincRNAs) are emerging as important regulators of diverse biological processes. However, our understanding of lincRNA abundance and function remains very limited especially for agriculturally important plants. Soybean is a major legume crop plant providing over a half of global oilseed production. Moreover, soybean can form symbiotic relationships with Rhizobium bacteria to fix atmospheric nitrogen. Soybean has a complex paleopolyploid genome and exhibits many vegetative and floral development complexities...
December 28, 2017: Plant Physiology
Xiang Sheng, Yury Patskovsky, Anna Vladimirova, Jeffrey B Bonanno, Steven C Almo, Fahmi Himo, Frank M Raushel
γ-Resorcylate decarboxylase (γ-RSD) has evolved to catalyze the reversible decarboxylation of 2,6-dihydroxybenzoate to resorcinol in a nonoxidative fashion. This enzyme is of significant interest because of its potential for the production of γ-resorcylate and other benzoic acid derivatives under environmentally sustainable conditions. Kinetic constants for the decarboxylation of 2,6-dihydroxybenzoate catalyzed by γ-RSD from Polaromonas sp. JS666 are reported and the enzyme is shown to be active with 2,3-dihydroxybenzoate, 2,4,6-trihydroxybenzoate and 2,6-dihydroxy-4 methylbenzoate...
December 28, 2017: Biochemistry
Antonio González-Sánchez, Ciro A Cubillas, Fabiola Miranda, Araceli Dávalos, Alejandro García-de Los Santos
Copper (Cu) is an essential micronutrient for all aerobic forms of life. Its oxidation states (Cu+ /Cu2+ ) make this metal an important cofactor of enzymes catalyzing redox reactions in essential biological processes. In gram-negative bacteria, Cu uptake is an unexplored component of a finely regulated trafficking network, mediated by protein-protein interactions that deliver Cu to target proteins and efflux surplus metal to avoid toxicity. Rhizobium etliCFN42 is a facultative symbiotic diazotroph that must ensure its appropriate Cu supply for living either free in the soil or as an intracellular symbiont of leguminous plants...
December 27, 2017: MicrobiologyOpen
Paula M Corsini, Kenneth T Walker, Joanne M Santini
Rhizobium sp. str. NT-26 is a Gram-negative facultative chemolithoautotrophic arsenite oxidizer that has been used as a model organism to study various aspects of arsenite oxidation including the regulation of arsenite oxidation. The three regulatory genes, aioX, aioS, and aioR, are cotranscribed when NT-26 was grown in the presence or absence of arsenite. The aioXSR operon is upregulated in stationary phase but not by the presence of arsenite in the growth medium. The two transcription start sites upstream of aioX were determined which led to the identification of two promoters, the housekeeping promoter RpoD and the growth-phase-dependent promoter RpoE2...
December 17, 2017: MicrobiologyOpen
Kamila Rachwał, Paulina Lipa, Iwona Wojda, José-María Vinardell, Monika Janczarek
Rhizobium leguminosarum bv. trifolii is a soil bacterium capable of establishing a symbiotic relationship with clover (Trifolium spp.). Previously, the rosR gene, encoding a global regulatory protein involved in motility, synthesis of cell-surface components, and other cellular processes was identified and characterized in this bacterium. This gene possesses a long upstream region that contains several regulatory motifs, including inverted repeats (IRs) of different lengths. So far, the role of these motifs in the regulation of rosR transcription has not been elucidated in detail...
December 15, 2017: Genes
Sheila McCormick
No abstract text is available yet for this article.
January 2018: Plant Journal: for Cell and Molecular Biology
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