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https://www.readbyqxmd.com/read/28516418/improvement-of-the-stabilization-and-activity-of-protocatechuate-3-4-dioxygenase-isolated-from-rhizobium-sp-lmb-1-and-immobilized-on-fe3o4-nanoparticles
#1
Li-Shuang Zhang, Yue Fang, Ying Zhou, Bang-Ce Ye
Protocatechuate 3,4-dioxygenase (P34O), which is isolated from Rhizobium sp. LMB-1, catalyzes the ring cleavage step in the metabolism of aromatic compounds, and has great potential for environmental bioremediation. However, its structure is very sensitive to different environmental factors, which weaken its activity. Immobilization of the enzyme can improve its stability, allow reusability, and reduce operation costs. In this work, the relative molecular mass of the native P34O enzyme was determined to be 500 kDa by gel filtration chromatography on Sephadex G-200, and the enzyme was immobilized onto (3-aminopropyl) triethoxysilane-modified Fe3O4 nanoparticles (NPs) by the glutaraldehyde method...
May 17, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28500544/diversity-and-plant-growth-promoting-properties-of-rhizobia-isolated-from-root-nodules-of-ononis-arvensis
#2
Sylwia Wdowiak-Wróbel, Monika Marek-Kozaczuk, Michał Kalita, Magdalena Karaś, Magdalena Wójcik, Wanda Małek
This is the first report describing isolates from root nodules of Ononis arvensis (field restharrow). The aim of this investigation was to describe the diversity, phylogeny, and plant growth promoting features of microsymbionts of O. arvensis, i.e., a legume plant growing in different places of the southern part of Poland. Twenty-nine bacterial isolates were characterized in terms of their phenotypic properties, genome fingerprinting, and comparative analysis of their 16S rRNA, nodC and acdS gene sequences...
May 12, 2017: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/28496149/the-active-site-structure-and-catalytic-mechanism-of-arsenite-oxidase
#3
Thomas P Warelow, M Jake Pushie, Julien J H Cotelesage, Joanne M Santini, Graham N George
Arsenite oxidase is thought to be an ancient enzyme, originating before the divergence of the Archaea and the Bacteria. We have investigated the nature of the molybdenum active site of the arsenite oxidase from the Alphaproteobacterium Rhizobium sp. str. NT-26 using a combination of X-ray absorption spectroscopy and computational chemistry. Our analysis indicates an oxidized Mo(VI) active site with a structure that is far from equilibrium. We propose that this is an entatic state imposed by the protein on the active site through relative orientation of the two molybdopterin cofactors, in a variant of the Rây-Dutt twist of classical coordination chemistry, which we call the pterin twist hypothesis...
May 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28495892/lux-bacterial-biosensors-for-in-vivo-spatiotemporal-mapping-of-root-secretion
#4
Francesco Pini, Alison K East, Corinne Appia-Ayme, Jakub Tomek, Ramakrishnan Karunakaran, Marcela Mendoza-Suarez, Anne Edwards, Jason Terpolilli, Joshua Rowoth, J Allan Downie, Phil Poole
Plants engineer the rhizosphere to their advantage by secreting various nutrients and secondary metabolites. Coupling transcriptomic and metabolomic analysis of the Pisum sativum rhizosphere, a suite of bioreporters has been developed in Rhizobium leguminosarum bv. viciae 3841, and these detect metabolites secreted by roots in space and time. Fourteen bacterial lux-fusion bioreporters, specific for sugars, polyols, amino acids, organic acids or flavonoids, have been validated in vitro and in vivo. Using different bacterial mutants (nodC, nifH), the process of colonization and symbiosis has been analyzed, revealing compounds important in the different steps of the rhizobial-legume association...
May 11, 2017: Plant Physiology
https://www.readbyqxmd.com/read/28489199/compatibility-of-azospirillum-brasilense-and-pseudomonas-fluorescens-in-growth-promotion-of-groundnut-arachis-hypogea-l
#5
Andhare A Prasad, Subramanian Babu
We attempted to study the compatibility among plant beneficial bacteria in the culture level by growing them near in the nutrient agar plates. Among all the bacteria tested, Rhizobium was found to inhibit the growth of other bacteria. From the compatible group of PGPR, we have selected one biofertilizer (Azospirillum brasilense strain TNAU) and one biocontrol agent (Pseudomonas fluorescens strain PF1) for further studies in the pot culture. We have also developed a bioformulation which is talc powder based, for individual bacteria and mixed culture...
May 2, 2017: Anais da Academia Brasileira de Ciências
https://www.readbyqxmd.com/read/28488698/the-rhizobium-tropici-ciat-899-nodd2-protein-regulates-the-production-of-nod-factors-under-salt-stress-in-a-flavonoid-independent-manner
#6
Pablo Del Cerro, Francisco Pérez-Montaño, Antonio Gil-Serrano, Francisco Javier López-Baena, Manuel Megías, Mariangela Hungria, Francisco Javier Ollero
In the symbiotic associations between rhizobia and legumes, NodD promotes the expression of the nodulation genes in the presence of appropriate flavonoids. This set of genes is implied in the synthesis of Nodulation factors, which are responsible for launching the nodulation process. Rhizobium tropici CIAT 899 is the most successful symbiont of Phaseolus vulgaris and can nodulate a variety of legumes. This strain produces Nodulation factors under abiotic stress such as acidity or high concentration of salt...
May 10, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28480491/double-genetically-modified-symbiotic-system-for-improved-cu-phytostabilization-in-legume-roots
#7
Patricia Pérez-Palacios, Asunción Romero-Aguilar, Julián Delgadillo, Bouchra Doukkali, Miguel A Caviedes, Ignacio D Rodríguez-Llorente, Eloísa Pajuelo
Excess copper (Cu) in soils has deleterious effects on plant growth and can pose a risk to human health. In the last decade, legume-rhizobium symbioses became attractive biotechnological tools for metal phytostabilization. For this technique being useful, metal-tolerant symbionts are required, which can be generated through genetic manipulation.In this work, a double symbiotic system was engineered for Cu phytostabilization: On the one hand, composite Medicago truncatula plants expressing the metallothionein gene mt4a from Arabidopsis thaliana in roots were obtained to improve plant Cu tolerance...
May 8, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28473386/permanent-draft-genome-sequence-of-rhizobium-sp-strain-lcm-4573-a-salt-tolerant-nitrogen-fixing-bacterium-isolated-from-senegalese-soils
#8
Nathalie Diagne, Erik Swanson, Céline Pesce, Fatoumata Fall, Fatou Diouf, Niokhor Bakhoum, Dioumacor Fall, Mathieu Ndigue Faye, Rediet Oshone, Stephen Simpson, Krystalynne Morris, W Kelley Thomas, Lionel Moulin, Diegane Diouf, Louis S Tisa
The genus Rhizobium contains many species that are able to form nitrogen-fixing nodules on plants of the legume family. Here, we report the 5.5-Mb draft genome sequence of the salt-tolerant Rhizobium sp. strain LCM 4573, which has a G+C content of 61.2% and 5,356 candidate protein-encoding genes.
May 4, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28469708/integrated-systems-for-biopolymers-and-bioenergy-production-from-organic-waste-and-by-products-a-review-of-microbial-processes
#9
REVIEW
Giorgia Pagliano, Valeria Ventorino, Antonio Panico, Olimpia Pepe
Recently, issues concerning the sustainable and harmless disposal of organic solid waste have generated interest in microbial biotechnologies aimed at converting waste materials into bioenergy and biomaterials, thus contributing to a reduction in economic dependence on fossil fuels. To valorize biomass, waste materials derived from agriculture, food processing factories, and municipal organic waste can be used to produce biopolymers, such as biohydrogen and biogas, through different microbial processes. In fact, different bacterial strains can synthesize biopolymers to convert waste materials into valuable intracellular (e...
2017: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/28469244/policing-the-legume-rhizobium-symbiosis-a-critical-test-of-partner-choice
#10
Annet Westhoek, Elsa Field, Finn Rehling, Geraldine Mulley, Isabel Webb, Philip S Poole, Lindsay A Turnbull
In legume-Rhizobium symbioses, specialised soil bacteria fix atmospheric nitrogen in return for carbon. However, ineffective strains can arise, making discrimination essential. Discrimination can occur via partner choice, where legumes prevent ineffective strains from entering, or via sanctioning, where plants provide fewer resources. Several studies have inferred that legumes exercise partner choice, but the rhizobia compared were not otherwise isogenic. To test when and how plants discriminate ineffective strains we developed sets of fixing and non-fixing strains that differed only in the expression of nifH - essential for nitrogen fixation - and could be visualised using marker genes...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28458146/insights-into-signal-transduction-by-a-hybrid-fixl-denaturation-study-of-on-and-off-states-of-a-multi-domain-oxygen-sensor
#11
Wellinson G Guimarães, Ana C S Gondim, Pedro Mikael da Silva Costa, Marie-Alda Gilles-Gonzalez, Luiz G F Lopes, Marta S P Carepo, Eduardo H S Sousa
FixL from Rhizobium etli (ReFixL) is a hybrid oxygen sensor protein. Signal transduction in ReFixL is effected by a switch off of the kinase activity on binding of an oxygen molecule to ferrous heme iron in another domain. Cyanide can also inhibit the kinase activity upon binding to the heme iron in the ferric state. The unfolding by urea of the purified full-length ReFixL in both active pentacoordinate form, met-FixL(Fe(III)) and inactive cyanomet-FixL (Fe(III)-CN(-)) form was monitored by UV-visible absorption spectroscopy, circular dichroism (CD) and fluorescence spectroscopy...
April 13, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28455332/absence-of-ppgpp-leads-to-increased-mobilization-of-intermediately-accumulated-poly-3-hydroxybutyrate-phb-in-ralstonia-eutropha-h16
#12
Janina R Jüngert, Marina Borisova, Christoph Mayer, Christiane Wolz, Christopher J Brigham, Anthony J Sinskey, Dieter Jendrossek
In this study, we constructed a set of single-, double-, and triple-gene deletion strains of Ralstonia eutropha H16 in (p)ppGpp synthase/hydrolase (spoT1), (p)ppGpp synthase (spoT2) and polyhydroxybutyrate (PHB) depolymerase (phaZa1 or phaZa3) genes and determined the impact on the levels of (p)ppGpp and on accumulated PHB. Double deletion mutants in both spoT1 and spoT2 genes were unable to synthesize detectable amounts of (p)ppGpp and accumulated only minor amounts of PHB due to PhaZa1-mediated depolymerization of PHB...
April 28, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28450855/the-abundance-of-endofungal-bacterium-rhizobium-radiobacter-syn-agrobacterium-tumefaciens-increases-in-its-fungal-host-piriformospora-indica-during-the-tripartite-sebacinalean-symbiosis-with-higher-plants
#13
Huijuan Guo, Stefanie P Glaeser, Ibrahim Alabid, Jafargholi Imani, Hossein Haghighi, Peter Kämpfer, Karl-Heinz Kogel
Rhizobium radiobacter (syn. Agrobacterium tumefaciens, syn. "Agrobacterium fabrum") is an endofungal bacterium of the fungal mutualist Piriformospora (syn. Serendipita) indica (Basidiomycota), which together form a tripartite Sebacinalean symbiosis with a broad range of plants. R. radiobacter strain F4 (RrF4), isolated from P. indica DSM 11827, induces growth promotion and systemic resistance in cereal crops, including barley and wheat, suggesting that R. radiobacter contributes to a successful symbiosis. Here, we studied the impact of endobacteria on the morphology and the beneficial activity of P...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28450528/draft-genome-sequence-of-rhizobiumpusense-strain-nrcpb10-t-lmg-25623-t-isolated-from-rhizosphere-soil-of-chickpeas-cicer-arietinum-l-grown-in-india
#14
Jhasketan Badhai, William B Whitman, Subrata K Das
Rhizobium pusense strain NRCPB10(T) was isolated from rhizosphere soil of chickpeas (Cicer arietinum L.). Based upon the draft genome sequence, the genome is 5.28 Mb and encodes 5,064 proteins.
April 27, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28447383/manganese-transport-is-essential-for-n2-fixation-by-rhizobium-leguminosarum-in-bacteroids-from-galegoid-but-not-phaseoloid-nodules
#15
Graham Hood, Vinoy Ramachandran, Alison East, J Allan Downie, Philip S Poole
Rhizobium leguminosarum has two high-affinity Mn(2+) transport systems encoded by sitABCD and mntH. In symbiosis, sitABCD and mntH were expressed throughout nodules and also strongly induced in Mn(2+) -limited cultures of free-living cells. Growth of a sitA mntH double mutant was severely reduced under Mn(2+) limitation and sitA and mntH single mutants were more sensitive to oxidative stress. The double sitA mntH mutant of R. leguminosarum was unable to fix nitrogen (Fix(-) ) with legumes belonging to the galegoid clade (Pisum sativum, Vicia faba and Vicia hirsuta)...
April 26, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28437159/specific-host-responsive-associations-between-medicago-truncatula-accessions-and-sinorhizobium-strains
#16
Théophile Kazmierczak, Marianna Nagymihály, Florian Lamouche, Quentin Barrière, Ibtissem Guefrachi, Benoit Alunni, Mouna Ouadghiri, Jamal Ibijbijen, Éva Kondorosi, Peter Mergaert, Véronique Gruber
Legume plants interact with rhizobia to form nitrogen-fixing root nodules. Legume-rhizobium interactions are specific and only compatible rhizobia and plant species will lead to nodule formation. Even within compatible interactions, the genotype of both the plant and the bacterial symbiont will impact on the efficiency of nodule functioning and nitrogen-fixation activity. The model legume Medicago truncatula forms nodules with several species of the Sinorhizobium genus. However, the efficiency of these bacterial strains is highly variable...
May 2017: Molecular Plant-microbe Interactions: MPMI
https://www.readbyqxmd.com/read/28421040/isolation-and-molecular-characterization-of-novel-chlorpyrifos-and-3-5-6-trichloro-2-pyridinol-degrading-bacteria-from-sugarcane-farm-soils
#17
Smriti Rayu, Uffe N Nielsen, Loïc Nazaries, Brajesh K Singh
Chlorpyrifos (CP) is one of the most widely used organophosphate pesticides in agriculture worldwide, but its extensive use has led to the contamination of various soil and water systems. Microbial bioremediation is considered to be one of the most viable options for the removal of CP from the environment; however, little is known about the soil bacterial diversity that degrade CP. Sequential soil and liquid culture enrichments enabled the isolation of bacterial CP degraders with sequence homologies to Xanthomonas sp...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28408686/permanent-draft-genome-sequence-of-the-french-bean-symbiont-rhizobium-sp-strain-rsm-3-isolated-from-the-eastern-himalayan-region-of-india
#18
Ritu Rai, Erik Swanson, Indrani Sarkar, Dorjay Lama, Feseha Abebe-Aleke, Stephen Simpson, Krystalynne Morris, W Kelley Thomas, Pallab Kar, Maher Gtari, Arnab Sen, Louis S Tisa
The genus Rhizobium contains many species able to form nitrogen-fixing nodules on plants of the legume family. Here, we report the 6.9-Mbp draft genome sequence of Rhizobium sp. strain RSm-3, with a G+C content of 61.4% and 6,511 candidate protein-coding genes.
April 13, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28405399/strain-identification-and-quorum-sensing-inhibition-characterization-of-marine-derived-rhizobium-sp-nao1
#19
Hong Chang, Jin Zhou, Xiaoshan Zhu, Shenchen Yu, Lu Chen, Hui Jin, Zhonghua Cai
A novel strategy for combating pathogens is through the ongoing development and use of anti-quorum sensing (QS) treatments such as therapeutic bacteria or their anti-QS substances. Relatively little is known about the bacteria that inhabit the open ocean and of their potential anti-pathogenic attributes; thus, in an initiative to identify these types of therapeutic bacteria, planktonic microbes from the North Atlantic Ocean were collected, isolated, cultured and screened for anti-QS activity. Screening analysis identified one such strain, Rhizobium sp...
March 2017: Royal Society Open Science
https://www.readbyqxmd.com/read/28398123/heterologous-complementation-reveals-a-specialized-activity-for-baca-in-the-medicago-sinorhizobium-meliloti-symbiosis
#20
George C diCenzo, Maryam Zamani, Hannah N Ludwig, Turlough M Finan
The bacterium Sinorhizobium meliloti Rm2011 forms N2-fixing root nodules on alfalfa and other leguminous plants. The pSymB chromid contains a 110-kb region (the ETR region) showing high synteny to a chromosomally located region in Sinorhizobium fredii NGR234 and related rhizobia. We recently introduced the ETR region from S. fredii NGR234 into the S. meliloti chromosome. Here, we report that, unexpectedly, the S. fredii NGR234 ETR region did not complement deletion of the S. meliloti ETR region in symbiosis with Medicago sativa...
April 2017: Molecular Plant-microbe Interactions: MPMI
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