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https://www.readbyqxmd.com/read/29241086/practical-aspects-of-the-automated-preparation-of-aqueous-two-phase-systems-for-the-analysis-of-biological-macromolecules
#1
Rana Hameed, Jonathan Huddleston, Svetlana Ignatova
A robust strategy for the automated preparation of aqueous two-phase systems (ATPS) using a liquid handling sample processor was developed using gravimetric methods: to determine the accuracy of preparation. The major robotic control parameters requiring adjustment were; speed of aspiration and dispense; delay times following aspiration and dispense alongside measures to control cross-contamination during phase sampling. In general mixture compositions of both polymer/polymer and polymer/salt mixtures could be prepared with a target bias accuracy of less than 5%...
October 3, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29240523/injectable-platelet-rich-fibrin-using-the-low-speed-centrifugation-concept-improves-cartilage-regeneration-when-compared-to-platelet-rich-plasma
#2
Mustafa Abd El Raouf, Xuzhu Wang, Si Miusi, Jihua Chai, Abdel Basit Mohamed AbdEl-Aal, Mekkawy M Nefissa Helmy, Shahram Ghanaati, Joseph Choukroun, Elisa Choukroun, Yufeng Zhang, Richard J Miron
The aim of the present study was to evaluate the effect of injectable platelet-rich fibrin (i-PRF) on cultivated chondrocytes and osteochondral regeneration in critical-sized osteochondral defect of the rabbit's knee in comparison to autologous platelet-rich plasma (PRP). Chondrocytes were first investigated for their ability to proliferate and differentiate in response to PRP and i-PRF. Thereafter, full-thickness critical-sized osteochondral defects 5 mm in diameter and 5 mm in depth were created in the knee joint of 12 adult female New Zealand White rabbits...
December 14, 2017: Platelets
https://www.readbyqxmd.com/read/29236984/longitudinal-association-between-brain-amyloid-beta-and-gait-in-the-mayo-clinic-study-of-aging
#3
Alexandra M V Wennberg, Timothy G Lesnick, Christopher G Schwarz, Rodolfo Savica, Clinton E Hagen, Rosebud O Roberts, David S Knopman, John H Hollman, Prashanthi Vemuri, Clifford R Jack, Ronald C Petersen, Michelle M Mielke
Background: The longitudinal association between cerebral amyloid-beta (Aβ) and change in gait, and whether this association is mediated by cortical thickness, has yet to be determined. Methods: We included 439 clinically normal (CN) participants, aged 50-69 years and enrolled in the Mayo Clinic Study of Aging with cerebral Aβ, cortical thickness, and gait measurements. Cerebral Aβ deposition was assessed by Pittsburgh Compound B (PiB)-PET in multiple regions of interest (ROIs) (i...
December 9, 2017: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
https://www.readbyqxmd.com/read/29230379/cross-cultural-and-hemispheric-laterality-effects-on-the-ensemble-coding-of-emotion-in-facial-crowds
#4
Hee Yeon Im, Sang Chul Chong, Jisoo Sun, Troy G Steiner, Daniel N Albohn, Reginald B Adams, Kestutis Kveraga
In many social situations, we make a snap judgment about crowds of people relying on their overall mood (termed "crowd emotion"). Although reading crowd emotion is critical for interpersonal dynamics, the sociocultural aspects of this process have not been explored. The current study examined how culture modulates the processing of crowd emotion in Korean and American observers. Korean and American (non-East Asian) participants were briefly presented with two groups of faces that were individually varying in emotional expressions and asked to choose which group between the two they would rather avoid...
October 2017: Culture and Brain
https://www.readbyqxmd.com/read/29229821/critical-dynamics-in-population-vaccinating-behavior
#5
A Demetri Pananos, Thomas M Bury, Clara Wang, Justin Schonfeld, Sharada P Mohanty, Brendan Nyhan, Marcel Salathé, Chris T Bauch
Vaccine refusal can lead to renewed outbreaks of previously eliminated diseases and even delay global eradication. Vaccinating decisions exemplify a complex, coupled system where vaccinating behavior and disease dynamics influence one another. Such systems often exhibit critical phenomena-special dynamics close to a tipping point leading to a new dynamical regime. For instance, critical slowing down (declining rate of recovery from small perturbations) may emerge as a tipping point is approached. Here, we collected and geocoded tweets about measles-mumps-rubella vaccine and classified their sentiment using machine-learning algorithms...
December 11, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29229343/heat-generation-during-bony-decompression-of-lumbar-spinal-stenosis-using-high-speed-diamond-drill-with-or-without-automated-irrigation-and-ultrasonic-bone-cutting-knife
#6
Marc Matthes, Dirk Thomas Pillich, Ehab El Refaee, Henry W S Schroeder, Jan-Uwe Müller
OBJECTIVE: The main objective of the present prospective randomized single-blinded controlled study was to measure heat during bony decompression of lumbar spinal stenosis with high-speed drills, and ultrasonic bone cutting knife. METHODS: 90 patients diagnosed lumbar spinal stenosis were included in this study, and randomized for lumbar spinal canal decompression using either a high-speed drill with automatic irrigation (HSA), high-speed drill with manual irrigation (HSM), or an ultrasonic bone cutting knife with automatic irrigation (USBCD)...
December 8, 2017: World Neurosurgery
https://www.readbyqxmd.com/read/29228409/short-term-effects-of-combined-high-intensity-strength-and-sprint-interval-training-on-anthropometric-characteristics-and-physical-performance-of-elite-team-handball-players
#7
Souhail Hermassi, Mohamed Souhail Chelly, Georg Fieseler, Thomas Bartels, Stephan Schulze, Karl-Stefan Delank, Roy J Shepard, René Schwesig
Muscular strength and speed are critical physical factors in determining the technical and tactical skills of elite team handball players. This study thus investigated the effect of high-intensity muscular strength and sprint interval training (SIT) on lower limb explosive performance and anthropometric characteristics in 22 male handball athletes aged 20.2 ± 0.1 years. A training group (TG, n = 12) and a control group (CG, n = 10) were followed over 8 weeks parallel to regular handball training...
December 2017: Sportverletzung Sportschaden: Organ der Gesellschaft Für Orthopädisch-Traumatologische Sportmedizin
https://www.readbyqxmd.com/read/29228162/prospective-memory-in-depression-review-of-an-emerging-field
#8
Craig P McFarland, Jennifer J Vasterling
Depressive disorders have been linked to a variety of neuropsychological deficits, including in the areas of processing speed, memory, and executive functioning. These neurocognitive disturbances may contribute to the impairments in daily functioning often experienced by those suffering with depression. Prospective memory (PM), which refers to remembering to execute a previously formed intention at some point in the future, has been shown to play a critical role in daily functioning and may be particularly relevant in the context of depression...
December 8, 2017: Archives of Clinical Neuropsychology: the Official Journal of the National Academy of Neuropsychologists
https://www.readbyqxmd.com/read/29228027/an-insulin-resistance-associated-neural-correlate-of-impulsivity-in-type-2-diabetes-mellitus
#9
Kristen L Eckstrand, Nishit Mummareddy, Hakmook Kang, Ronald Cowan, Minchun Zhou, David Zald, Heidi J Silver, Kevin D Niswender, Malcolm J Avison
Central insulin resistance (IR) influences striatal dopamine (DA) tone, an important determinant of behavioral self-regulation. We hypothesized that an association exists between the degree of peripheral IR and impulse control, mediated by the impact of IR on brain circuits controlling the speed of executing "go" and/or "stop" responses. We measured brain activation and associated performance on a stop signal task (SST) in obese adults with type 2 diabetes (age, 48.1 ± 6.9 yrs (mean ± SD); BMI, 36.5 ± 4...
2017: PloS One
https://www.readbyqxmd.com/read/29226613/large-scale-culture-of-a-megakaryocytic-progenitor-cell-line-with-a-single-use-bioreactor-system
#10
Retno Wahyu Nurhayati, Yoshihiro Ojima, Takeaki Dohda, Masahiro Kino-Oka
The increasing application of regenerative medicine has generated a growing demand for stem cells and their derivatives. Single-use bioreactors offer an attractive platform for stem cell expansion owing to their scalability for large-scale production and feasibility of meeting clinical-grade standards. The current work evaluated the capacity of a single-use bioreactor system (1 L working volume) for expanding Meg01 cells, a megakaryocytic (MK) progenitor cell line. Oxygen supply was provided by surface aeration to minimize foaming and orbital shaking was used to promote oxygen transfer...
December 11, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/29223876/joint-moments-required-to-hold-a-posture-while-somersaulting
#11
Joanne Mikl
A pure somersault is a key skill in diving and gymnastics, and involves rotation about the transverse axis of the body. As the rotational speed increases the effort required to maintain a specified posture increases. This paper derives equations for the joint moments required for an athlete to hold three sport specific postures as a function of rotational speed. The joint moment is related to the isometric muscular strength and is the limiting factor of an athlete in their ability to hold a fixed posture while somersaulting...
December 7, 2017: Human Movement Science
https://www.readbyqxmd.com/read/29220753/a-systematic-review-of-the-gait-characteristics-associated-with-cerebellar-ataxia
#12
REVIEW
Ellen Buckley, Claudia Mazzà, Alisdair McNeill
BACKGROUND: Cerebellar Ataxias are a group of gait disorders resulting from dysfunction of the cerebellum, commonly characterised by slowly progressing incoordination that manifests as problems with balance and walking leading to considerable disability. There is increasing acceptance of gait analysis techniques to quantify subtle gait characteristics that are unmeasurable by current clinical methods This systematic review aims to identify the gait characteristics able to differentiate between Cerebellar Ataxia and healthy controls...
December 1, 2017: Gait & Posture
https://www.readbyqxmd.com/read/29219343/revision-of-bubble-bursting-universal-scaling-laws-of-top-jet-drop-size-and-speed
#13
Alfonso M Gañán-Calvo
The collapse of a bubble of radius R_{o} at the surface of a liquid generating a liquid jet and a subsequent first drop of radius R is universally scaled using the Ohnesorge number Oh=μ/(ρσR_{o})^{1/2} and a critical value Oh^{*} below which no droplet is ejected; ρ, σ, and μ are the liquid density, surface tension, and viscosity, respectively. First, a flow field analysis at ejection yields the scaling of R with the jet velocity V as R/l_{μ}∼(V/V_{μ})^{-5/3}, where l_{μ}=μ^{2}/(ρσ) and V_{μ}=σ/μ...
November 17, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/29218225/the-gill-parasite-paramoeba-perurans-compromises-aerobic-scope-swimming-capacity-and-ion-balance-in-atlantic-salmon
#14
Malthe Hvas, Egil Karlsbakk, Stig Mæhle, Daniel William Wright, Frode Oppedal
The parasitic amoeba Paramoeba perurans is an aetiological agent of amoebic gill disease (AGD), a serious problem in seawater salmonid aquaculture globally. Other finfish species are also infected and infection events may be associated with periods of unusual high temperatures. Currently little is known about the impact of AGD on wild fish, but in a time with global warming and increasing aquaculture production this potential threat could be on the rise. A better understanding of the pathophysiology of infected fish is therefore warranted...
2017: Conservation Physiology
https://www.readbyqxmd.com/read/29214028/reprogramming-of-a-defense-signaling-pathway-in-rough-lemon-and-sweet-orange-is-a-critical-element-of-the-early-response-to-candidatus-liberibacter-asiaticus
#15
Qibin Yu, Chunxian Chen, Dongliang Du, Ming Huang, Jiqiang Yao, Fahong Yu, Ronald H Brlansky, Frederick G Gmitter
Huanglongbing (HLB) in citrus infected by Candidatus Liberibacter asiaticus (CLas) has caused tremendous losses to the citrus industry. No resistant genotypes have been identified in citrus species or close relatives. Among citrus varieties, rough lemon (Citrus jambhiri) has been considered tolerant due to its ability to produce a healthy flush of new growth after infection. The difference between tolerance and susceptibility is often defined by the speed and intensity of a plant's response to a pathogen, especially early defense responses...
2017: Horticulture Research
https://www.readbyqxmd.com/read/29212842/lean-mean-lipolytic-machines-lipid-mobilization-in-rainbow-trout-during-graded-swimming
#16
Eric D Turenne, Jean-Michel Weber
The mobilization of mammalian lipid reserves is strongly stimulated during exercise to reach a maximum at moderate intensities, but the effects of swimming speed on fish lipolysis have never been quantified. Continuous infusion of 2-[3H]glycerol was used to measure Ra glycerol (=rate of appearance of glycerol or lipolytic rate) in rainbow trout kept at rest, or during graded exercise in a swim tunnel up to critical swimming speed (Ucrit). Results show that Ra glycerol is 1.67±0.18 µmol kg-1min-1 in control animals, and remains at a steady level of 1...
December 6, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29208996/relativistic-inverse-compton-scattering-of-photons-from-the-early-universe
#17
Siddharth Malu, Abhirup Datta, Sergio Colafrancesco, Paolo Marchegiani, Ravi Subrahmanyan, D Narasimha, Mark H Wieringa
Electrons at relativistic speeds, diffusing in magnetic fields, cause copious emission at radio frequencies in both clusters of galaxies and radio galaxies through non-thermal radiation emission called synchrotron. However, the total power radiated through this mechanism is ill constrained, as the lower limit of the electron energy distribution, or low-energy cutoffs, for radio emission in galaxy clusters and radio galaxies, have not yet been determined. This lower limit, parametrized by the lower limit of the electron momentum - pmin - is critical for estimating the total energetics of non-thermal electrons produced by cluster mergers or injected by radio galaxy jets, which impacts the formation of large-scale structure in the universe, as well as the evolution of local structures inside galaxy clusters...
December 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29208898/diffusion-of-treatment-in-social-networks-and-mass-drug-administration
#18
Goylette F Chami, Andreas A Kontoleon, Erwin Bulte, Alan Fenwick, Narcis B Kabatereine, Edridah M Tukahebwa, David W Dunne
Information, behaviors, and technologies spread when people interact. Understanding these interactions is critical for achieving the greatest diffusion of public interventions. Yet, little is known about the performance of starting points (seed nodes) for diffusion. We track routine mass drug administration-the large-scale distribution of deworming drugs-in Uganda. We observe friendship networks, socioeconomic factors, and treatment delivery outcomes for 16,357 individuals in 3491 households of 17 rural villages...
December 5, 2017: Nature Communications
https://www.readbyqxmd.com/read/29208077/response-time-consistency-is-an-indicator-of-executive-control-rather-than-global-cognitive-ability
#19
Brandon P Vasquez, Malcolm A Binns, Nicole D Anderson
OBJECTIVES: Intraindividual variability increases with age, but the relative strength of association with cognitive domains is still unclear. The objective of this study was to examine the relation between cognitive domains and the shape and spread of response time (RT) distributions as indexed by intraindividual standard deviation (ISD), and ex-Gaussian parameters (μ, σ, τ). METHODS: Healthy adults (40 young [aged 18-30 years], 40 young-old [aged 65-74 years], and 41 old-old [aged 75-85 years]) completed neuropsychological testing and a touch-screen attention task from which ISD and ex-Gaussian parameters were derived...
December 6, 2017: Journal of the International Neuropsychological Society: JINS
https://www.readbyqxmd.com/read/29206624/use-of-highways-in-the-sky-and-a-virtual-pad-for-landing-head-up-display-symbology-to-enable-improved-helicopter-pilots-situation-awareness-and-workload-in-degraded-visual-conditions
#20
Neville A Stanton, Katherine L Plant, Aaron P Roberts, Craig K Allison
Flight within degraded visual conditions is a great challenge to pilots of rotary-wing craft. Environmental cues typically used to guide interpretation of speed, location and approach can become obscured, forcing the pilots to rely on data available from in-cockpit instrumentation. To ease the task of flight during degraded visual conditions, pilots require easy access to flight critical information. The current study examined the effect of "Highways in the Sky" symbology and a conformal virtual pad for landing presented using a Head Up Display (HUD) on pilots' workload and situation awareness for both clear and degraded conditions across a series of simulated rotary-wing approach and landings...
December 5, 2017: Ergonomics
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