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https://www.readbyqxmd.com/read/28427249/crystal-structure-of-the-regulatory-domain-of-aphb-from-vibrio-vulnificus-a-virulence-gene-regulator
#1
Nohra Park, Saemee Song, Garam Choi, Kyung Ku Jang, Inseong Jo, Sang Ho Choi, Nam-Chul Ha
The transcriptional activator AphB has been implicated in acid resistance and pathogenesis in the food borne pathogens Vibrio vulnificus and Vibrio cholerae. To date, the full-length AphB crystal structure of V. cholerae has been determined and characterized by a tetrameric assembly of AphB consisting of a DNA binding domain and a regulatory domain (RD). Although acidic pH and low oxygen tension might be involved in the activation of AphB, it remains unknown which ligand or stimulus activates AphB at the molecular level...
April 20, 2017: Molecules and Cells
https://www.readbyqxmd.com/read/28396124/lactobacilli-and-pediococci-as-versatile-cell-factories-evaluation-of-strain-properties-and-genetic-tools
#2
REVIEW
Elleke F Bosma, Jochen Forster, Alex Toftgaard Nielsen
This review discusses opportunities and bottlenecks for cell factory development of Lactic Acid Bacteria (LAB), with an emphasis on lactobacilli and pediococci, their metabolism and genetic tools. In order to enable economically feasible bio-based production of chemicals and fuels in a biorefinery, the choice of product, substrate and production organism is important. Currently, the most frequently used production hosts include Escherichia coli and Saccharomyces cerevisiae, but promising examples are available of alternative hosts such as LAB...
April 7, 2017: Biotechnology Advances
https://www.readbyqxmd.com/read/28394487/graphene-decorated-with-uniform-ultrathin-cop-x-fep-1-x-nanorods-a-robust-non-noble-metal-catalyst-for-hydrogen-evolution
#3
Baocang Liu, Lili Huo, Zhiqing Gao, Guolei Zhi, Geng Zhang, Jun Zhang
Developing high-performance but low-cost hydrogen evolution reaction (HER) electrocatalysts with superior activity and stability for future sustainable energy conversion technologies is highly desired. Tuning of microstructure, configuration, and chemical composition are paramount to developing effective non-noble electrocatalysts for HER. Herein, a universal "nanocasting" method is reported to construct graphene decorated with uniform ternary (CoP)x -(FeP)1-x (0 ≤ x ≤ 1) nanorods hybrids with different chemical compositions [(CoP)x -(FeP)1-x -NRs/G] as a highly active and durable nonprecious-metal electrocatalyst for the HER...
April 10, 2017: Small
https://www.readbyqxmd.com/read/28386249/identification-and-characterization-of-a-novel-salt-tolerant-esterase-from-the-deep-sea-sediment-of-the-south-china-sea
#4
Yi Zhang, Jie Hao, Yan-Qi Zhang, Xiu-Lan Chen, Bin-Bin Xie, Mei Shi, Bai-Cheng Zhou, Yu-Zhong Zhang, Ping-Yi Li
Marine esterases play an important role in marine organic carbon degradation and cycling. Halotolerant esterases from the sea may have good potentials in industrial processes requiring high salts. Although a large number of marine esterases have been characterized, reports on halotolerant esterases are only a few. Here, a fosmid library containing 7,200 clones was constructed from a deep-sea sediment sample from the South China Sea. A gene H8 encoding an esterase was identified from this library by functional screening and expressed in Escherichia coli...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28378582/low-affinity-zinc-sensor-showing-fluorescence-responses-with-minimal-artifacts
#5
Xinhao Yan, Jin Ju Kim, Hye Sun Jeong, Yu Kyung Moon, Yoon Kyung Cho, Soyeon Ahn, Sang Beom Jun, Hakwon Kim, Youngmin You
The study of the zinc biology requires molecular probes with proper zinc affinity. We developed a low-affinity zinc probe (HBO-ACR) based on an azacrown ether (ACR) and an 2-(2-hydroxyphenyl)benzoxazole (HBO) fluorophore. This probe design imposed positive charge in the vicinity of a zinc coordination center, which enabled fluorescence turn-on responses to high levels of zinc without being affected by the pH and the presence of other transition-metal ions. Steady-state and transient photophysical investigations suggested that such a high tolerance benefits from orchestrated actions of proton-induced nonradiative and zinc-induced radiative control...
April 5, 2017: Inorganic Chemistry
https://www.readbyqxmd.com/read/28372761/inhibition-factors-and-kinetic-model-for-ammonium-inhibition-on-the-anammox-process-of-the-snad-biofilm
#6
Zhaoming Zheng, Jun Li, Jing Ma, Jia Du, Fan Wang, Wei Bian, Yanzhuo Zhang, Baihang Zhao
The aim of the present work was to evaluate the anaerobic ammonium oxidation (anammox) activity of simultaneous partial nitrification, anammox and denitrification (SNAD) biofilm with different substrate concentrations and pH values. Kaldnes rings taken from the SNAD biofilm reactor were incubated in batch tests to determine the anammox activity. Haldane model was applied to investigate the ammonium inhibition on anammox process. As for nitrite inhibition, the NH4(+)-N removal rate of anammox process remained 87...
March 2017: Journal of Environmental Sciences (China)
https://www.readbyqxmd.com/read/28365797/bacillus-sphaericus-lmg-22257-is-physiologically-suitable-for-self-healing-concrete
#7
Jianyun Wang, Henk M Jonkers, Nico Boon, Nele De Belie
The suitability of using a spore-forming ureolytic strain, Bacillus sphaericus, was evaluated for self-healing of concrete cracks. The main focus was on alkaline tolerance, calcium tolerance, oxygen dependence, and low-temperature adaptability. Experimental results show that B. sphaericus had a good tolerance. It can grow and germinate in a broad range of alkaline pH. The optimal pH range is 7 ∼ 9. High alkaline conditions (pH 10 ∼ 11) slow down but not stop the growth and germination. Oxygen was strictly needed during bacterial growth and germination, but not an essential factor during bacterial urea decomposition...
April 1, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28364869/a-vaginal-estradiol-softgel-capsule-tx-004hr-has-negligible-to-very-low-systemic-absorption-of-estradiol-efficacy-and-pharmacokinetic-data-review
#8
REVIEW
James A Simon, David F Archer, Ginger D Constantine, James H Pickar, Julia M Amadio, Brian Bernick, Shelli Graham, Sebastian Mirkin
This paper reviews the efficacy, safety, and systemic absorption of estradiol with TX-004HR, an investigational, low-dose 17β-estradiol vaginal softgel capsule, designed to treat vulvar and vaginal atrophy (VVA) in postmenopausal women, with an improved user experience. In phase 2 (NCT02449902) and phase 3 REJOICE (NCT02253173) studies, TX-004HR significantly improved the proportions of vaginal superficial and parabasal cells and vaginal pH, and in the phase 3 study decreased the severity of dyspareunia, vaginal dryness, and vulvar and/or vaginal itching or irritation...
May 2017: Maturitas
https://www.readbyqxmd.com/read/28364708/evaluation-of-buffers-toxicity-in-tobacco-cells-homopiperazine-1-4-bis-2-ethanesulfonic-acid-is-a-suitable-buffer-for-plant-cells-studies-at-low-ph
#9
Lucélia Borgo
Low pH is an important environmental stressor of plant root cells. Understanding the mechanisms of stress and tolerance to acidity is critical; however, there is no widely accepted pH buffer for studies of plant cells at low pH. Such a buffer might also benefit studies of Al toxicity, in which buffering at low pH is also important. The challenge is to find a buffer with minimal cellular effects. We examined the cytotoxicity and possible metabolic disturbances of four buffers that have adequate pKa values and potential use for studies in the pH range of 4...
March 19, 2017: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/28358306/effect-of-the-biofilm-age-and-starvation-on-acid-tolerance-of-biofilm-formed-by-streptococcus-mutans-isolated-from-caries-active-and-caries-free-adults
#10
Shan Jiang, Shuai Chen, Chengfei Zhang, Xingfu Zhao, Xiaojing Huang, Zhiyu Cai
Streptococcus mutans (S. mutans) is considered a leading cause of dental caries. The capability of S. mutans to tolerate low pH is essential for its cariogenicity. Aciduricity of S. mutans is linked to its adaptation to environmental stress in oral cavity. This study aimed to investigate the effect of biofilm age and starvation condition on acid tolerance of biofilm formed by S. mutans clinical isolates. S. mutans clinical strains isolated from caries-active (SM593) and caries-free (SM18) adults and a reference strain (ATCC25175) were used for biofilm formation...
March 30, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28357544/a-new-source-of-resistance-to-2-furaldehyde-from-scheffersomyces-pichia-stipitis-for-sustainable-lignocellulose-to-biofuel-conversion
#11
Xu Wang, Z Lewis Liu, Xiaoping Zhang, Menggen Ma
Aldehyde inhibitory compounds derived from lignocellulosic biomass pretreatment have been identified as a major class of toxic chemicals that interfere with microbial growth and subsequent fermentation for advanced biofuel production. Development of robust next-generation biocatalyst is a key for a low-cost biofuel production industry. Scheffersomyces (Pichia) stipitis is a naturally occurring C-5 sugar utilization yeast; however, little is known about the genetic background underlying its potential tolerance to biomass conversion inhibitors...
March 29, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28349873/cadmium-spiked-soil-modulates-root-organic-acids-exudation-and-ionic-contents-of-two-differentially-cd-tolerant-maize-zea-mays-l-cultivars
#12
M Tariq Javed, M Sohail Akram, Kashif Tanwir, Hassan Javed Chaudhary, Qasim Ali, Eva Stoltz, Sylvia Lindberg
Our earlier work described that the roots of two maize cultivars, grown hydroponically, differentially responded to cadmium (Cd) stress by initiating changes in medium pH depending on their Cd tolerance. The current study investigated the root exudation, elemental contents and antioxidant behavior of the same maize cultivars [cv. 3062 (Cd-tolerant) and cv. 31P41 (Cd-sensitive)] under Cd stress. Plants were maintained in a rhizobox-like system carrying soil spiked with Cd concentrations of 0, 10, 20, 30, 40 and 50 μmol/kg soil...
March 24, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28316047/exogenous-application-of-plant-growth-regulators-pgrs-induces-chilling-tolerance-in-short-duration-hybrid-maize
#13
Muhammad Ahmed Waqas, Imran Khan, Muhammad Javaid Akhter, Mehmood Ali Noor, Umair Ashraf
Chilling stress hampers the optimal performance of maize under field conditions precipitously by inducing oxidative stress. To confer the damaging effects of chilling stress, the present study aimed to investigate the effects of some natural and synthetic plant growth regulators, i.e., salicylic acid (SA), thiourea (TU), sorghum water extract (SWE), and moringa leaf extract (MLE) on chilling stress tolerance in autumn maize hybrid. Foliar application of growth regulators at low concentrations was carried out at six leaf (V6) and tasseling stages...
March 18, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28315928/boosting-the-growth-of-the-probiotic-strain-lactobacillus-paracasei-ssp-paracasei-f19
#14
Desideria Brignone, Pia Radmann, Jürgen Behr, Rudi F Vogel
Single so-called booster substances were added to the fermentation medium of the probiotic strain Lactobacillus (L.) paracasei ssp. paracasei F19 to enhance its growth. A wide screening was carried out in microtiter plates and a statistical analysis of the growth parameters was performed. CFU counts were used to correlate the increase in OD590nm with the increase in viable cell number. Sodium ascorbate, sodium pyruvate, manganese sulfate and cysteine had a remarkable boosting effect on the growth of L. paracasei F19...
March 18, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28293865/the-inhibition-effect-of-lactobacilli-against-growth-and-biofilm-formation-of-pseudomonas-aeruginosa
#15
Dariush Shokri, Mohammad Rabbani Khorasgani, Milad Mohkam, Seyed Masih Fatemi, Younes Ghasemi, Asghar Taheri-Kafrani
The emergence of antibiotic-resistant and food-spoilage microorganisms has renewed efforts to identify safe and natural alternative agents of antibiotics such as probiotics. The aim of this study was the isolation of lactobacilli as potential probiotics from local dairy products with broad antibacterial and anti-biofilm activities against antibiotic-resistant strains of Pseudomonas aeruginosa and determination of their inhibition mechanism. Antibiotic susceptibility and classification of acquired resistance profiles of 80 P...
March 14, 2017: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/28293217/mechanistic-insights-underlying-tolerance-to-acetic-acid-stress-in-vaginal-candida-glabrata-clinical-isolates
#16
Diana V Cunha, Sara B Salazar, Maria M Lopes, Nuno P Mira
During colonization of the vaginal tract Candida glabrata cells are challenged with the presence of acetic acid at a low pH, specially when dysbiosis occurs. To avoid exclusion from this niche C. glabrata cells are expected to evolve efficient adaptive responses to cope with this stress; however, these responses remain largely uncharacterized, especially in vaginal strains. In this work a cohort of 18 vaginal strains and 2 laboratory strains (CBS138 and KUE100) were phenotyped for their tolerance against inhibitory concentrations of acetic acid at pH 4...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28285657/two-component-regulatory-system-acts-actr-is-required-for-sinorhizobium-meliloti-adaptation-to-oxidative-stress
#17
Guirong Tang, Sunjun Wang, Dawei Lu, Leqi Huang, Ningning Li, Li Luo
The two-component system ActS/ActR plays important roles in bacterial adaptation to abiotic stress, including acid tolerance and oxidant resistance. However, the underlying regulatory mechanism is not clear. In this study, we found that the ActS/ActR system is required for adaptation to oxidative stress by regulating the transcription of the genes actR, katB, gshA and gshB1. The actS and actR mutants were sensitive to low pH and oxidants such as H2O2, oxidized glutathione (GSSG) and sodium nitroprusside (SNP)...
May 2017: Microbiological Research
https://www.readbyqxmd.com/read/28278249/expression-of-c-5-sterol-desaturase-from-an-edible-mushroom-in-fisson-yeast-enhances-its-ethanol-and-thermotolerance
#18
Ayushi Kamthan, Mohan Kamthan, Asis Datta
Bioethanol is an environment friendly and renewable source of energy produced by the fermentation of agricultural raw material by a variety of microorganisms including yeast. Obtaining yeast strains that are tolerant to stresses like high levels of ethanol and high temperature is highly desirable as it reduces cost and increases yield during bioethanol production. Here, we report that heterologous expression of C-5 Sterol desaturase (FvC5SD)-an ergosterol biosynthesis enzyme from an edible mushroom Flammulina velutipes in fission yeast, not only imparts increased thermotolerance but also tolerance towards high ethanol concentration and low pH...
2017: PloS One
https://www.readbyqxmd.com/read/28270819/effects-of-low-ph-on-photosynthesis-related-physiological-parameters-and-nutrient-profiles-of-citrus
#19
An Long, Jiang Zhang, Lin-Tong Yang, Xin Ye, Ning-Wei Lai, Ling-Ling Tan, Dan Lin, Li-Song Chen
Seedlings of "Xuegan" (Citrus sinensis) and "Sour pummelo" (Citrus grandis) were irrigated daily with a nutrient solution at a pH of 2.5, 3, 4, 5, or 6 for 9 months. Thereafter, the following responses were investigated: seedling growth; root, stem, and leaf concentrations of nutrient elements; leaf gas exchange, pigment concentration, ribulose-1,5-bisphosphate carboxylase/oxygenase activity and chlorophyll a fluorescence; relative water content, total soluble protein level, H2O2 production and electrolyte leakage in roots and leaves...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28265263/genome-sequence-of-desulfurella-amilsii-strain-tr1-and-comparative-genomics-of-desulfurellaceae-family
#20
Anna P Florentino, Alfons J M Stams, Irene Sánchez-Andrea
The acidotolerant sulfur reducer Desulfurella amilsii was isolated from sediments of Tinto River, an extremely acidic environment. Its ability to grow in a broad range of pH and to tolerate certain heavy metals offers potential for metal recovery processes. Here we report its high-quality draft genome sequence and compare it to the available genome sequences of other members of Desulfurellaceae family: D. acetivorans. D. multipotens, Hippea maritima. H. alviniae, H. medeae, and H. jasoniae. For most species, pairwise comparisons for average nucleotide identity (ANI) and in silico DNA-DNA hybridization (DDH) revealed ANI values from 67...
2017: Frontiers in Microbiology
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