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https://www.readbyqxmd.com/read/28736979/in-vitro-characteristics-of-lactobacillus-spp-strains-isolated-from-the-chicken-digestive-tract-and-their-role-in-the-inhibition-of-campylobacter-colonization
#1
Patrycja A Kobierecka, Agnieszka K Wyszyńska, Tamara Aleksandrzak-Piekarczyk, Maciej Kuczkowski, Anna Tuzimek, Wioletta Piotrowska, Adrian Górecki, Iwona Adamska, Alina Wieliczko, Jacek Bardowski, Elżbieta K Jagusztyn-Krynicka
Campylobacter jejuni/coli infections are the leading cause of bacterial diarrheal illnesses in humans. Many epidemiological studies indicate that improperly prepared meat from chickens that carry a high load of Campylobacter in their intestinal tracts is the key source of human infections. LAB, mainly members of the Lactococcus and Lactobacillus genera, increasingly have been tested as vehicles for the delivery of heterologous bacterial or viral antigens to animal mucosal immune systems. Thus, the objective of this study was to isolate, identify, and characterize Lactobacillus spp...
July 24, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28736550/probiotic-enterococcus-mundtii-isolate-protects-the-model-insect-tribolium-castaneum-against-bacillus-thuringiensis
#2
Thorben Grau, Andreas Vilcinskas, Gerrit Joop
Enterococcus mundtii strains isolated from the larval feces of the Mediterranean flour moth Ephestia kuehniella show antimicrobial activity against a broad spectrum of Gram-positive and Gram-negative bacteria. The in vitro probiotic characterization of one isolate revealed a high auto-aggregation score, a hydrophilic cell surface, tolerance for low pH, no hemolytic activity, and susceptibility to all tested antibiotics. We used the red flour beetle Tribolium castaneum, an established model organism, for the in vivo characterization of one probiotic E...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28733934/draft-genome-sequence-of-the-deep-sea-bacterium-moritella-sp-jt01-and-identification-of-biotechnologically-relevant-genes
#3
Robert Cardoso de Freitas, Estácio Jussie Odisi, Chiaki Kato, Marcus Adonai Castro da Silva, André Oliveira de Souza Lima
Deep-sea bacteria can produce various biotechnologically relevant enzymes due to their adaptations to high pressures and low temperatures. To identify such enzymes, we have sequenced the genome of the polycaprolactone-degrading bacterium Moritella sp. JT01, isolated from sediment samples from Japan Trench (6957 m depth), using a Illumina HiSeq2000 sequencer (12.1 million paired-end reads) and CLC Genomics Workbench (version 6.5.1) for the assembly, resulting in a 4.83-Mb genome (42 scaffolds). The genome was annotated using Rapid Annotation using Subsystem Technology (RAST), Protein Homology/analogY Recognition Engine V 2...
July 22, 2017: Marine Biotechnology
https://www.readbyqxmd.com/read/28726711/the-use-of-constructed-wetlands-for-the-treatment-and-reuse-of-urban-wastewater-for-the-irrigation-of-two-warm-season-turfgrass-species-under-mediterranean-climatic-conditions
#4
Mario Licata, Teresa Tuttolomondo, Claudio Leto, Salvatore La Bella, Giuseppe Virga
Constructed wetlands (CWs) represent low-cost technology for the treatment and reuse of wastewater in urban areas. This study aimed to evaluate the pollutant removal efficiency of a CW system and to assess the effects of irrigation using treated urban wastewater on soil and on two warm-season turf species. The research was carried out in Sicily (Italy) on a pilot-scale horizontal subsurface flow system which was fed with treated urban wastewater following secondary treatment from an activated-sludge wastewater treatment plant...
July 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/28713415/repeated-stand-replacing-crown-fires-affect-seed-morphology-and-germination-in-aleppo-pine
#5
Antonio Saracino, Alessandro Bellino, Emilia Allevato, Antonio Mingo, Stefano Conti, Sergio Rossi, Giuliano Bonanomi, Domenico Carputo, Stefano Mazzoleni
Post-fire reproductive niche of Aleppo pine (Pinus halepensis) is deeply interlaced with fire products. Indeed, the high pH and low osmotic potentials of ash beds under burnt crowns constitute the main constraints to seed germination. In this study, we aim to investigate whether fire recurrence, through the physico-chemical constraints imposed by the ash beds, affects the reproduction ability of P. halepensis at the germination stage. To this aim, Aleppo pine seeds were collected in neighboring even-aged stands subjected to 0, 1, or 2 fires (namely fire cohorts), and seed morphology and germination performance, in terms of cumulative germination and germination kinetics, were studied under increasing osmotic potentials (from 0...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28710262/med15b-regulates-acid-stress-response-and-tolerance-in-candida-glabrata
#6
Yanli Qi, Hui Liu, Jiayin Yu, Xiulai Chen, Liming Liu
Candida glabrata is a promising producer of organic acids. To elucidate the physiological function of the Mediator tail subunit Med15B in the response to low pH stress, we constructed a deletion strain, med15BΔ, and overexpression strain, HTUΔ/CgMED15B Deletion of MED15B caused biomass production, glucose consumption rate, and cell viability to decrease by 28.3%, 31.7%, and 26.5%, respectively, compared with those of the parent strain (HTUΔ) at pH 2.0. Expression of lipid metabolism-related genes was significantly downregulated in the med15BΔ strain, whereas key genes of ergosterol biosynthesis showed abnormal upregulation...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28705771/different-frequencies-of-porphyromonas-gingivalis-infection-in-cancers-of-the-upper-digestive-tract
#7
Xiang Yuan, Yiwen Liu, Jinyu Kong, Bianli Gu, Yijun Qi, Xinshuai Wang, Man Sun, Pan Chen, Wei Sun, Huizhi Wang, Fuyou Zhou, Shegan Gao
The high incidence rate of multiple carcinomas in the upper digestive tract is often explained in terms of involvement of the same underlying risk factors. It has been reported that the oral bacterium Streptococcus anginosus is associated with esophageal, gastric, and pharyngeal cancers. We previously reported occurrence of Porphyromonas gingivalis (P. gingivalis) DNA in esophagus cancer. In this study, the presence of P. gingivalis in specimens of various types of cancer from the upper digestive tract was investigated...
July 11, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28690100/arginase-purified-from-endophytic-pseudomonas-aeruginosa-ih2-induce-apoptosis-through-both-cell-cycle-arrest-and-mmp-loss-in-human-leukemic-hl-60%C3%A2-cells
#8
Islam Husain, Kiran Bala, Abubakar Wani, Ubaid Makhdoomi, Fayaz Malik, Anjana Sharma
Arginase is a therapeutic enzyme for arginine-auxotrophic cancers but their low anticancer activity, less proteolytic tolerance and shorter serum half-life are the major shortcomings. In this study, arginase from Pseudomonas aeruginosa IH2 was purified to homogeneity and estimated as 75 kDa on native-PAGE and 37 kDa on SDS-PAGE. Arginase showed optimum activity at pH 8 and temperature 35 °C. Mn(2+) and Mg(2+) ions enhanced arginase activity while, Li(+), Cu(2+), and Al(3+) ions reduced arginase activity...
July 6, 2017: Chemico-biological Interactions
https://www.readbyqxmd.com/read/28689267/the-novel-srna-s015-improves-nisin-yield-by-increasing-acid-tolerance-of-lactococcus-lactis-f44
#9
Jiakun Qi, Qinggele Caiyin, Hao Wu, Kairen Tian, Binbin Wang, Yanni Li, Jianjun Qiao
Nisin, a polycyclic antibacterial peptide produced by Lactococcus lactis, is stable at low pH. Improving the acid tolerance of L. lactis could thus enhance nisin yield. Small non-coding RNAs (sRNAs) play essential roles in acid tolerance by regulating their target mRNAs at the post-transcriptional level. In this study, a novel sRNA, s015, was identified in L. lactis F44 via the use of RNA sequencing, qRT-PCR analysis, and Northern blotting. s015 improved the acid tolerance of L. lactis and boosted nisin yield at low pH...
July 9, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28683398/polyethyleneimine-protected-silver-nanoclusters-luminescence-probe-for-sensitive-detection-of-cobalt-ii-in-living-cells
#10
Lei Meng, Quan Zhu, Jian-Hang Yin, Na Xu
A simple luminescence sensor, based on polyethyleneimine protected silver nanoclusters (AgNCs@PEI) is successfully fabricated via one-pot reduction method. The obtained AgNCs@PEI are characterized by high-resolution transmission electron microscopy (HR-TEM), Dynamic light scattering (DLS), transient and steady-state fluorescence, and UV-vis spectroscopy. The NCs show large Stocks-shift (∼130nm), high tolerability to extreme pH and high ionic strengths, and excellent photo-stability under UV irradiation, laying the basement for the practical applications...
June 29, 2017: Journal of Photochemistry and Photobiology. B, Biology
https://www.readbyqxmd.com/read/28681289/diversity-and-functional-properties-of-acid-tolerant-bacteria-isolated-from-tea-plantation-soil-of-assam
#11
Gunajit Goswami, Priyadarshini Deka, Pompi Das, Sudipta Sankar Bora, Ramkrishna Samanta, Robin Chandra Boro, Madhumita Barooah
In this study, we report on the bacterial diversity and their functional properties prevalent in tea garden soils of Assam that have low pH (3.8-5.5). Culture-dependent studies and phospholipid fatty acid analysis revealed a high abundance of Gram-positive bacteria. Further, 70 acid-tolerant bacterial isolates characterized using a polyphasic taxonomy approach could be grouped to the genus Bacillus, Lysinibacillus, Staphylococcus, Brevundimonas, Alcaligenes, Enterobacter, Klebsiella, Escherichia, and Aeromonas...
July 2017: 3 Biotech
https://www.readbyqxmd.com/read/28680127/the-discovery-of-novel-heat-stable-keratinases-from-meiothermus-taiwanensis-wr-220-and-other-extremophiles
#12
Wan-Ling Wu, Mei-Yi Chen, I-Fan Tu, Yu-Ching Lin, Nadendla EswarKumar, Ming-Yi Chen, Meng-Chiao Ho, Shih-Hsiung Wu
Billions of tons of keratin bio-wastes are generated by poultry industry annually but discarded that result in serious environmental pollution. Keratinase is a broad spectrum protease with the unique ability to degrade keratin, providing an eco-friendly way to convert keratin wastes to valuable amino acids. In this report, a feather-degrading thermophilic bacterium, Meiothermus taiwanensis WR-220, was investigated due to its ability to apparently complete feather decay at 65 °C in two days. By genomics, proteomics, and biochemical approaches, the extracellular heat-stable keratinase (MtaKer) from M...
July 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28677149/isolating-and-evaluating-lactic-acid-bacteria-strains-for-effectiveness-on-silage-quality-at-low-temperatures-on-the-tibetan-plateau
#13
REVIEW
Siran Wang, Xianjun Yuan, Zhihao Dong, Junfeng Li, Tao Shao
Four lactic acid bacteria (LAB) strains isolated from straw silages on the Tibetan Plateau were characterized, and their effects on the fermentation quality of Italian ryegrass (Lolium multiflorum Lam.) at different temperatures (10°C, 15°C and 25°C) were studied. These LAB isolates were evaluated using the acids production ability test, morphological observation, Gram staining, physiological, biochemical and acid tolerance tests. All the isolates (M1, LM8, LO7 and LOG9) could grow at 5-20°C, pH 3.5-7.0 and NaCl (3...
July 5, 2017: Animal Science Journal, Nihon Chikusan Gakkaihō
https://www.readbyqxmd.com/read/28667115/cgmed3-changes-membrane-sterol-composition-to-help-candida-glabrata-tolerate-low-ph-stress
#14
Xiaobao Lin, Yanli Qi, Dongni Yan, Hui Liu, Xiulai Chen, Liming Liu
Candida glabrata is a promising microorganism for organic acid production. The present study aimed to investigate the role of Mediator complex subunit 3 (CgMed3p) in protecting C. glabrata at low pH conditions. To this end, the genes CgMED3A and CgMED3B were deleted, resulting in the double deletion Cgmed3ABΔ strain. Final biomass and cell viability of Cgmed3ABΔ decreased by 64.5% and 35.8%, respectively, compared to the wild-type at pH 2.0. In addition, lack of CgMed3ABp resulted in selective repression of a subset of genes in the lipid biosynthesis and metabolism pathways...
June 30, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28666069/setting-the-dry-weight-and-its-cardiovascular-implications
#15
Arjun D Sinha, Rajiv Agarwal
Volume overload is common and associated with adverse outcomes in the hemodialysis population including systemic hypertension, pulmonary hypertension, left ventricular hypertrophy, and mortality. Since the beginning of the era of maintenance dialysis, prescribing and maintaining a dry weight remains the standard of care for managing volume overload on hemodialysis. Reducing dry weight even by relatively small amounts has been shown to improve blood pressure and has been associated with reductions in left ventricular hypertrophy...
June 30, 2017: Seminars in Dialysis
https://www.readbyqxmd.com/read/28666017/potential-of-eucalyptus-camaldulensis-for-phytostabilization-and-biomonitoring-of-trace-element-contaminated-soils
#16
Paula Madejón, Teodoro Marañón, Carmen M Navarro-Fernández, María T Domínguez, José M Alegre, Brett Robinson, José M Murillo
Soil pollution by trace elements (TEs) from mining and industrial activity is widespread and presents a risk to humans and ecosystems. The use of trees to immobilize TEs (phytostabilization) is a low-cost and effective method of soil remediation. We aimed to determine the chemical composition of leaves and flower buds of Eucalyptus camaldulensis in seven sites along the Guadiamar River valley (SW Spain), an area contaminated by a mine-spill in 1998. E. camaldulensis trees in the spill-affected area and adjacent non affected areas were growing on a variety of soils with pH from 5...
2017: PloS One
https://www.readbyqxmd.com/read/28659931/establishment-growth-and-yield-potential-of-the-perennial-grass-miscanthus-%C3%A3-giganteus-on-degraded-coal-mine-soils
#17
Stanisław Jeżowski, Michal Mos, Sam Buckby, Joanna Cerazy-Waliszewska, Wojciech Owczarzak, Andrzej Mocek, Zygmunt Kaczmarek, Jon P McCalmont
Miscanthus × giganteus is a giant C4 grass native to Asia. Unlike most C4 species, it is relatively cold tolerant due to adaptations across a wide range of altitudes. These grasses are characterized by high productivity and low input requirements, making them excellent candidates for bioenergy feedstock production. The aim of this study was to investigate the potential for growing Miscanthus on extremely marginal soils, degraded by open lignite (brown coal) mining. Field experiments were established within three blocks situated on waste heaps originating from the lignite mine...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28648288/characterization-of-bacillus-megaterium-bacillus-pumilus-and-paenibacillus-polymyxa-isolated-from-a-pinot-noir-wine-from-western-washington-state
#18
Nicolas H von Cosmos, Bruce A Watson, J K Fellman, D S Mattinson, Charles G Edwards
This report provides the first confirmed evidence of Bacillus-like bacteria present in a wine from Washington State. These bacteria were isolated from a 2013 Pinot noir wine whose aroma was sensorially described as being 'dirty' or 'pond scum.' Based on physiological traits and genetic sequencing, three bacterial isolates were identified as Bacillus megaterium (strain NHO-1), Bacillus pumilus (strain NHO-2), and Paenibacillus polymyxa (strain NHO-3). These bacteria grew in synthetic media of low pH (pH 3.5) while some survived ethanol concentrations up to 15% v/v...
October 2017: Food Microbiology
https://www.readbyqxmd.com/read/28647525/catalytic-hydrolysis-of-starch-for-biohydrogen-production-by-using-a-newly-identified-amylase-from-a-marine-bacterium-catenovulum-sp-x3
#19
Yi-Rui Wu, Aihua Mao, Chongran Sun, Sabarathinam Shanmugam, Jin Li, Mingqi Zhong, Zhong Hu
An identified cold-adaptive, organic solvents-tolerant alkaline α-amylase (HP664) from Catenovulum sp. strain X3 was heterologously expressed and characterized in E. coli, and it was further applied to starch saccharification for biohydrogen production. The recombinant HP664 belongs to a member of glycoside hydrolase family 13 (GH13), with a molecular weight of 69.6kDa without signal peptides, and also shares a relatively low similarity (49%) to other reported amylases. Biochemical characterization demonstrated that the maximal enzymatic activity of HP664 was observed at 35°C and pH 9...
June 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28642761/overexpression-of-bdmate-gene-improves-aluminum-tolerance-in-setaria-viridis
#20
Ana P Ribeiro, Wagner R de Souza, Polyana K Martins, Felipe Vinecky, Karoline E Duarte, Marcos F Basso, Bárbara A D B da Cunha, Raquel B Campanha, Patrícia A de Oliveira, Danilo C Centeno, Geraldo M A Cançado, Jurandir V de Magalhães, Carlos A F de Sousa, Alan C Andrade, Adilson K Kobayashi, Hugo B C Molinari
Acidic soils are distributed worldwide, predominantly in tropical and subtropical areas, reaching around 50% of the arable soil. This type of soil strongly reduces crop production, mainly because of the presence of aluminum, which has its solubility increased at low pH levels. A well-known physiological mechanism used by plants to cope with Al stress involves activation of membrane transporters responsible for organic acid anions secretion from the root apex to the rhizosphere, which chelate Al, preventing its absorption by roots...
2017: Frontiers in Plant Science
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