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Low ph tolerance

Naiyu Wang, Can Wang
The release of cyanobacterial toxins during algal bloom has adverse effects on aquatic plants and animals. This study aimed to understand the toxic effects and mechanism of microcystin-LR (MC-LR) on the seedling growth and physiological responses of Iris pseudacorus L. (calamus). After a one-month exposure experiment, the growth and development of the calamus leaves were significantly inhibited, and this inhibitory effect was verified to be concentration dependent. Furthermore, the cell membrane system was damaged, and the photosynthesis was also adversely affected by MC-LR...
April 27, 2018: Aquatic Toxicology
Julio Guerrero-Castro, Luis Lozano, Christian Sohlenkamp
Rhizobium tropici CIAT899 is a nodule-forming α-proteobacterium displaying intrinsic resistance to several abiotic stress conditions such as low pH and high temperatures, which are common in tropical environments. It is a good competitor for Phaseolus vulgaris (common bean) nodule occupancy at low pH values, however little is known about the genetic and physiological basis of the tolerance to acidic conditions. To identify genes in R. tropici involved in pH stress response we combined two different approaches: (1) A Tn 5 mutant library of R...
2018: Frontiers in Microbiology
Peter Andráš, João Xavier Matos, Ingrid Turisová, Maria João Batista, Radoslava Kanianska, Sherif Kharbish
São Domingos belongs among the most important historic Iberian Pyrite Belt Cu mines. The anthrosoil is contaminated by a very high content of heavy metals and metalloids. The study was focused on evaluating the interaction of some chemical elements (Ca, Mg, Fe, Mn, Cu, Pb, Zn, Ag, Cd, Ni, Co, As, Sb) in the system soil vs. five autochthonous dominant plant species: Pinus pinaster Aiton, Quercus rotundifolia Lam., Agrostis sp., Juncus conglomeratus L. and Juncus effusus L. The plants are heavily contaminated by Cu, Pb, As and Zn...
May 11, 2018: Environmental Science and Pollution Research International
Xin Yan, Jun-Wei Huang, Xi-Hui Xu, Dian Chen, Xiang-Ting Xie, Qing Tao, Jian He, Jian-Dong Jiang
The synergistic relationships between plants and their rhizospheric microbes can be used to develop a combinational bioremediation method, overcoming the constraints of individual phytoremediation or bioaugmentation method. Here, we provide a combinational transgenic plant-microbial remediation system for a more efficient removal of phenylurea herbicides (PHs) from contaminated-sites. The transgenic Arabidopsis thaliana synthesizing the bacterial N -demethylase PdmAB in the chloroplast was developed. The constructed transgenic Arabidopsis exhibited significant tolerance to isoproturon (IPU), a typical PH, and it took up the IPU through roots and transported to leaves, where the majority of the IPU was demethylated to 3-(4-isopropylphenyl)-1-methylurea (MDIPU)...
May 11, 2018: Applied and Environmental Microbiology
Peng Guo, Yi-Ping Qi, Yan-Tong Cai, Tao-Yu Yang, Lin-Tong Yang, Zeng-Rong Huang, Li-Song Chen
Citrus are mainly grown in low pH soils with high active aluminum (Al). 'Xuegan' (Citrus sinensis (L.) Osbeck) and 'Shatian pummelo' (Citrus grandis (L.) Osbeck) seedlings were fertilized for 18 weeks with nutrient solution containing either 0 mM (control) or 1 mM (Al toxicity) AlCl3·6H2O. Aluminum induced decreases of biomass, leaf photosynthesis, relative water content and total soluble protein levels, and increases of methylglyoxal levels only occurred in C. grandis roots and leaves. Besides, the Al-induced decreases of pigments and alterations of chlorophyll a fluorescence transients and fluorescence parameters were greater in C...
April 27, 2018: Tree Physiology
Chen Chen, Linfeng Wu, Qinghua Cao, Huanhuan Shao, Xuedan Li, Yizheng Zhang, Haiyan Wang, Xuemei Tan
Zymomonas mobilis has the special Entner-Doudoroff (ED) pathway and it has excellent industrial characteristics, including low cell mass formation, high-specific productivity,ethanol yield, notable ethanol tolerance and wide pH range, a relatively small genome size. In this study, the genome sequences of NRRL B-14023 and NRRL B-12526 were sequenced and compared with other strains to explore their evolutionary relationships and the genetic basis of Z. mobilis. The comparative genomic analyses revealed that the 8 strains share a conserved core chromosomal backbone...
2018: PloS One
Ram Hari Dahal, Dhiraj Kumar Chaudhary, Jaisoo Kim
A novel bacterium, designated strain E9T , was isolated from pine forest soil of Kyonggi University (Suwon, Republic of Korea). Cells were facultatively anaerobic, Gram-staining-negative, catalase-negative, oxidase-positive, non-motile, non-spore-forming, rod-shaped and straw coloured. Prosthecae were absent. Glucose was fermented. The strain grew in the pH range of 5.0-10.0 (optimum, 6.5-8.5) and at 45 °C (optimum, 28-32 °C). E9T was sensitive to NaCl at low concentration and tolerated only 0.2 % NaCl (w/v)...
April 23, 2018: International Journal of Systematic and Evolutionary Microbiology
Rachit Kashyap, Rohit Sharma, Sanjay Kr Uniyal
Loss of green cover, and increasing pollution is a prime global concern. The problem calls for screening of pollution-tolerant tree species that can be integrated into plantation drives. Recognizing this, the study analyzed bio-indicator responses and performance of commonly occurring plant species along a pollution gradient in western Himalaya. Based on distance from the road, three sites viz., highly polluted (HP), moderately polluted (MP), and least polluted (LP), were identified. From these sites, leaves of commonly occurring 26 tree species were collected and analyzed for dust accumulation, total chlorophyll, relative water content (RWC), ascorbic acid, and pH using standard protocols...
April 21, 2018: Environmental Monitoring and Assessment
Wandee Sirichokchatchawan, Puwiya Pupa, Prasert Praechansri, Nutthee Am-In, Somboon Tanasupawat, Piengchan Sonthayanon, Nuvee Prapasarakul
Lactic acid bacteria (LAB) play an important role in pig health and performance that arises from their beneficial impacts on the balance of gastrointestinal microbes, ability to fight enteric pathogens, and capacity to support the immune system. The aim of this study was to evaluate the functional and safety aspects of five previously isolated autochthonous LAB strains, (Lactobacillus plantarum 22F, 25F and 31F, Pediococcus acidilactici 72N and Pediococcus pentosaceus 77F) from pig faeces as potential probiotics for a pig feed supplement...
April 17, 2018: Microbial Pathogenesis
Daojin Li, Hongjun Xia, Lin Wang
Boronate affinity materials have attracted more and more attention in extraction, separation and enrichment of glycoproteins due to the important roles that glycoproteins take on in recent years. However, conventional boronate affinity materials suffer from low binding affinity mainly because of the use of single boronic acids. This makes the extraction of glycoproteins of trace concentration become rather difficult or impossible. Here we present a novel boronate avidity material, polyethyleneimine (PEI)-assisted boronic acid-functionalized silica nanoparticles (SNPs)...
July 1, 2018: Talanta
Alexandra Kotogán, Carolina Zambrano, Anita Kecskeméti, Mónika Varga, András Szekeres, Tamás Papp, Csaba Vágvölgyi, Miklós Takó
Lipase enzymes of the oleaginous fungal group Mortierella are rarely studied. However, considering that most commercial lipases are derived from filamentous fungal sources, their investigation can contribute to the cost-effective development of new biotechnological processes. Here, an extracellular lipase with a molecular mass of 30 kDa was isolated from Mortierella echinosphaera CBS 575.75 and characterized. The purified lipase exhibited an optimal p -nitrophenyl palmitate ( p NPP)-hydrolyzing activity at 25 °C and pH 6...
April 10, 2018: International Journal of Molecular Sciences
Jacob B Campbell, Mads Kuhlmann Andersen, Johannes Overgaard, Jon F Harrison
Oxygen limitation plays a key role in many pathologies; yet, we still lack a fundamental understanding of the mechanisms responsible for variation in anoxia tolerance. Most vertebrate studies suggest that anoxia tolerance involves the ability to maintain cellular ATP despite the loss of aerobic metabolism. However, insects such as adult D. melanogaster are able to survive long periods of anoxia (LT50: ∼8 h) in a hypo-energetic state characterized by low [ATP]. In this study, we tested for possible mechanisms that allow D...
April 3, 2018: Journal of Experimental Biology
Jian Li, Xu-Fei Huang, Qian-Qian Cai, Chen Wang, Hao Cai, Hao Zhao, Lu Zhang, Xin-Xin Cao, Robert Peter Gale, Dao-Bin Zhou
POEMS syndrome is a rare plasma dyscrasia without standard treatment. This phase II prospective trial evaluates the safety and response of 12 cycles of low dose lenalidomide (10mg) plus dexamethasone (Rdex) in patients with newly diagnosed POEMS syndrome. Forty-one patients (28 men) were enrolled and the median age at diagnosis was 49 years (range, 21-70 years). Twenty-one patients (46%) achieved complete hematologic response and the neurologic response rate was 95%. The median serum VEGF declined from 5155 pg/ml (range, 534-14328 pg/ml) to 832 pg/ml (95-6254 pg/ml) after therapy...
March 31, 2018: American Journal of Hematology
Mingxing Tian, Yanqing Bao, Peng Li, Hai Hu, Chan Ding, Shaohui Wang, Tao Li, Jingjing Qi, Xiaolan Wang, Shengqing Yu
Brucellosis is a zoonotic bacterial disease caused by Brucella spp. The virulence of these bacteria is dependent on their ability to invade and replicate within host cells. In a previous study, a putative gene bab_RS27735 encoding an amino acid ABC transporter substrate-binding protein homologous to AapJ protein was found to be involved in Brucella abortus virulence. In this study, we successfully constructed a bab_RS27735 deletion mutant, Δ27735. Compared with the wild-type strain, the lipopolysaccharide pattern of the mutant was not changed, but the growth ability was slightly defected in the exponential phase...
March 29, 2018: Veterinary Research
Guohui Sun, Xin Zhang, Zixian Bao, Xuqian Lang, Zhongzheng Zhou, Yang Li, Chao Feng, Xiguang Chen
To strengthen the mechanical strength of thermo-sensitive hydroxybutyl chitosan (HBC) hydrogel, chitin whiskers were used as sticker to fabricate reinforced HBC (HBCW) hydrogel by using response surface methodology. Unlike the intrinsic network of HBC hydrogel, HBCW hydrogel showed a laminar shape with firm structure. The preparation condition was optimized by three-factor-three-level Box-Behnken design. The maximum mechanical strength (1011.11 Pa) was achieved at 50 °C, when the concentrations of HBC and chitin whiskers were 5...
June 1, 2018: Carbohydrate Polymers
Tamara Aleksandrzak-Piekarczyk, Weronika Puzia, Joanna Żylińska, Jarosław Cieśla, Krzysztof A Gulewicz, Jacek K Bardowski, Roman K Górecki
The aim of this study was to characterize and compare selected Lactobacillus strains originating from different environments (cow milk and hen feces) with respect to their applicative potential to colonize gastrointestinal track of chickens before hatching from an egg. In vitro phenotypic characterization of lactobacilli strains included the investigation of the important prerequisites for persistence in gastrointestinal tract, such as a capability to survive in the presence of bile salts and at low pH, enzymatic and sugar metabolic profiles, adhesion abilities, and resistance to osmolytes, temperature, and antibiotics...
March 25, 2018: MicrobiologyOpen
Fahime Mohammadi, Morteza Eshaghi, Shabnam Razavi, Davood Darban Sarokhalil, Malihe Talebi, Mohammad Reza Pourshafie
The purpose of the present study was to isolate Lactobacillus bacteria from mother's milk and to assess their probiotic potential. Sixty breast milk samples were collected from the volunteered mothers aged from 19 to 35 and from rural areas of Lorestan and Markazi Provinces, Iran. At first, 970 bacill-shaped bacterial colonies were isolated from these samples and stored in proper condition. Two hundred isolates were randomly selected and investigated for their ability to tolerate acidic condition and to tolerate bile salt as well...
March 15, 2018: Microbial Pathogenesis
Chunyan Chen, Ni Sun, Dongsheng Li, Sihua Long, Xiaoyu Tang, Guoqing Xiao, Linyuan Wang
Kitchen waste oil (KWO) from catering industries or households was used as a low-cost carbon source for producing biosurfactants by self-isolated Pseudomonas aeruginosa. Fermentation performance with KWO was superior to those with four other carbon sources, with higher optical density (OD600 ) of 2.33 and lower interfacial tension of 0.57 mN/m. Culture conditions for biosurfactant production were optimized, with optimal pH of 8.0 and nitrogen source concentration of 2.0 g/L, respectively. The results of infrared spectroscopy and liquid chromatography-mass spectrometry (LC-MS) showed that the biosurfactant was a mixture of six rhamnolipid congeners, among which Rha-Rha-C10 -C10 and Rha-C10 -C10 were the main components, with mass fraction of approximately 34...
March 16, 2018: Environmental Science and Pollution Research International
Jiao Jiang, Krista M Sumby, Joanna F Sundstrom, Paul R Grbin, Vladimir Jiranek
High concentrations of ethanol, low pH, the presence of sulfur dioxide and some polyphenols have been reported to inhibit Oenococcus oeni growth, thereby negatively affecting malolactic fermentation (MLF) of wine. In order to generate superior O. oeni strains that can conduct more efficient MLF, despite these multiple stressors, a continuous culture approach was designed to directly evolve an existing ethanol tolerant O. oeni strain, A90. The strain was grown for ∼350 generations in a red wine-like environment with increasing levels of stressors...
August 2018: Food Microbiology
Yuqin Shi, Jun Liu, Qiaojuan Yan, Xin You, Shaoqing Yang, Zhengqiang Jiang
Prebiotic effects of curdlan (1 → 3)-β-d-glucan oligosaccharides (GOS) were examined. GOS was tolerant against simulated gastrointestinal digestion, as well as low pH, thermal, and Maillard reaction conditions likely occurred during food processing. Growth of tested Lactobacillus (L.) strains was improved by GOS except L. brevis NRRL B-4527. E. coli did not grow on GOS as the only carbon source. In vitro batch fermentation using human faecal microbiota showed that GOS significantly increased the population of Lactobacillus sp...
May 15, 2018: Carbohydrate Polymers
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