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https://www.readbyqxmd.com/read/28634885/colorimetric-determination-of-pb-2-in-perfect-aqueous-solution-using-carminic-acid-as-a-selective-chemosensor
#1
Masaru Sakamaki, Shunichi Aikawa, Yasumasa Fukushima
The commercially available natural organic dye, carminic acid (CA), an anthraquinone derivative bearing hydroxyl and carboxyl groups as recognition sites was found to be a colorimetric probe for Pb(2+) in perfect aqueous solution under neutral conditions with specific selectivity and high sensitivity. Upon addition of Pb(2+), the absorption maximum of CA showed a large red shift, and the resulted color change from red to purple could be easily identified even by the naked eye. The chemical stoichiometric ratio between CA and Pb(2+) was determined to be 1:2 through Job plot, Pb(2+) titration, and kinetic experiments...
June 21, 2017: Journal of Fluorescence
https://www.readbyqxmd.com/read/28630637/evaluation-of-circulating-srage-in-osteoporosis-according-to-bmi-adipokines-and-fracture-risk-a-pilot-observational-study
#2
Emanuela Galliera, Monica Gioia Marazzi, Carmine Gazzaruso, Pietro Gallotti, Adriana Coppola, Tiziana Montalcini, Arturo Pujia, Massimiliano M Corsi Romanelli
BACKGROUND: Osteoporosis is a systemic metabolic disease based on age-dependent imbalance between the rates of bone formation and bone resorption. Recent studies on the pathogenesis of this disease identified that bone remodelling impairment, at the base of osteoporotic bone fragility, could be related to protein glycation, in association to oxidative stress. The glycation reactions lead to the generation of glycation end products (AGEs) which, in turn, accumulates into bone, where they binds to the receptor for AGE (RAGE)...
2017: Immunity & Ageing: I & A
https://www.readbyqxmd.com/read/28630469/c2238-anp-gene-variant-promotes-increased-platelet-aggregation-through-the-activation-of-nox2-and-the-reduction-of-camp
#3
Roberto Carnevale, Pasquale Pignatelli, Giacomo Frati, Cristina Nocella, Rosita Stanzione, Daniele Pastori, Simona Marchitti, Valentina Valenti, Maria Santulli, Emanuele Barbato, Teresa Strisciuglio, Leonardo Schirone, Carmine Vecchione, Francesco Violi, Massimo Volpe, Speranza Rubattu, Sebastiano Sciarretta
Subjects carrying the C2238 variant of the atrial natriuretic peptide (ANP) gene have a higher occurrence of stroke and acute coronary syndrome, suggesting an increased predisposition to acute thrombotic events in these subjects. We evaluated for the first time the direct effects of mutant ANP (C2238/αANP) on platelet activation in vitro and in human subjects. In vitro, platelets were incubated with no peptide, with T2238/αANP (WT) or with C2238/αANP at different concentrations. C2238/αANP (10(-10) M) induced higher collagen-induced platelet aggregation with respect to both control without ANP and T2238/αANP...
June 19, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28625511/lung-fluid-clearance-in-chronic-heart-failure-patients
#4
Dani-Louise Dixon, Carmine G De Pasquale, Mark D Lawrence, Elena Cavallaro, Vito Rubino, Andrew D Bersten
Chronic elevation of pulmonary microvascular pressure (Pmv) consistently leads to alveolocapillary barrier thickening and reduction in the filtration coefficient. In animal models of chronic heart failure (CHF) the lung remains dry despite hydrostatic forces. As fluid flux is bi-directional, it has been postulated that an increase in alveolar fluid clearance may facilitate the dry lung when Pmv is chronically elevated. In this study we aimed to examine alveolar fluid clearance in ambulatory patients with CHF secondary to left ventricular (LV) systolic dysfunction compared against non-CHF controls...
May 26, 2017: International Journal of Cardiology
https://www.readbyqxmd.com/read/28625450/vasoplegic-syndrome-an-update-on-perioperative-considerations
#5
REVIEW
Henry Liu, Ling Yu, Longqiu Yang, Michael S Green
Vasoplegic syndrome (VS) is increasingly recognized as an important clinical entity in perioperative medicine. VS is characterized by significant arterial hypotension, normal or high cardiac output, low systemic vascular resistance, and increased requirements for intravenous volume and vasopressors. Tremendous variations exist regarding incidence reported in the literature and management at different institutions; and the incidence of VS is likely significantly higher than many anesthesiologists believe. Thus the aims of this article are to review the pertinent aspects related to VS and alert clinical anesthesiologists to this under-recognized yet very challenging clinical condition...
August 2017: Journal of Clinical Anesthesia
https://www.readbyqxmd.com/read/28619945/transcriptional-activation-of-ragd-gtpase-controls-mtorc1-and-promotes-cancer-growth
#6
Chiara Di Malta, Diletta Siciliano, Alessia Calcagni, Jlenia Monfregola, Simona Punzi, Nunzia Pastore, Andrea N Eastes, Oliver Davis, Rossella De Cegli, Angela Zampelli, Luca G Di Giovannantonio, Edoardo Nusco, Nick Platt, Alessandro Guida, Margret Helga Ogmundsdottir, Luisa Lanfrancone, Rushika M Perera, Roberto Zoncu, Pier Giuseppe Pelicci, Carmine Settembre, Andrea Ballabio
The mechanistic target of rapamycin complex 1 (mTORC1) is recruited to the lysosome by Rag guanosine triphosphatases (GTPases) and regulates anabolic pathways in response to nutrients. We found that MiT/TFE transcription factors-master regulators of lysosomal and melanosomal biogenesis and autophagy-control mTORC1 lysosomal recruitment and activity by directly regulating the expression of RagD. In mice, this mechanism mediated adaptation to food availability after starvation and physical exercise and played an important role in cancer growth...
June 16, 2017: Science
https://www.readbyqxmd.com/read/28618587/general-scaling-relations-for-locomotion-in-granular-media
#7
James Slonaker, D Carrington Motley, Qiong Zhang, Stephen Townsend, Carmine Senatore, Karl Iagnemma, Ken Kamrin
Inspired by dynamic similarity in fluid systems, we have derived a general dimensionless form for locomotion in granular materials, which is validated in experiments and discrete element method (DEM) simulations. The form instructs how to scale size, mass, and driving parameters in order to relate dynamic behaviors of different locomotors in the same granular media. The scaling can be derived by assuming intrusion forces arise from resistive force theory or equivalently by assuming the granular material behaves as a continuum obeying a frictional yield criterion...
May 2017: Physical Review. E
https://www.readbyqxmd.com/read/28617883/ruling-in-and-ruling-out-significant-fibrosis-and-cirrhosis-in-patients-with-chronic-hepatitis-c-using-a-shear-wave-measurement-method
#8
Giovanna Ferraioli, Laura Maiocchi, Raffaella Lissandrin, Carmine Tinelli, Annalisa De Silvestri, Carlo Filice
AIMS: To prospectively assess the cutoff values of a point shear wave measurement (SWM) method for ruling-in and ruling-out significant fibrosis and cirrhosis using transient elastography (TE) as the reference standard. METHOD: Consecutive patients with chronic hepatitis C were enrolled. Liver stiffness was assessed with the SWM method implemented on the HI VISION Ascendus ultrasound system (Hitachi Ltd, Japan) and with the TE method of the FibroScan® device (Echosens, France)...
June 2017: Journal of Gastrointestinal and Liver Diseases: JGLD
https://www.readbyqxmd.com/read/28614107/multifactorial-dermatitis-with-probable-systemic-contact-dermatitis-to-carmine
#9
Gina J Ferris, Margaret Wat, Susan Nedorost
No abstract text is available yet for this article.
June 13, 2017: Dermatitis
https://www.readbyqxmd.com/read/28602192/mediated-electrochemical-method-for-the-determination-of-indigo-carmine-levels-in-food-products
#10
Majid Arvand, Mehrnoosh Saberi, Masoomeh Sayyar Ardaki, Asadollah Mohammadi
In this paper, we have studied the electrochemical behavior of indigo carmine (IC) in the presence and absence of mediator molecules. We have used three azo dyes as mediators in our studies and the effect of mediator molecules on the electron transfer between the IC solution and the electrode surface was explained from the cyclic voltammograms data. Among these dyes, 4-(4-nitrophenilazo)N-benzyl,N-ethylaniline (NBE) showed good performance as an electron transfer mediator. The NBE/carbon paste was characterized with Fourier transform infrared spectroscopy and scanning electron microscopy methods...
October 1, 2017: Talanta
https://www.readbyqxmd.com/read/28601383/pretreatment-serum-concentration-of-vitamin-d-and-breast-cancer-characteristics-a-prospective-observational-mediterranean-study
#11
Giuseppe Buono, Mario Giuliano, Carmine De Angelis, Rossella Lauria, Valeria Forestieri, Matilde Pensabene, Dario Bruzzese, Sabino De Placido, Grazia Arpino
BACKGROUND: Recent studies of the correlation between breast cancer (BC) and vitamin D yielded contrasting results. Although preclinical and clinical evidence has implicated vitamin D in BC prevention and outcome, little is known about the link between vitamin D and specific BC histologically defined subtypes. In the attempt to clarify this association we correlated vitamin D levels with BC characteristics. PATIENTS AND METHODS: We enrolled 220 pre- and postmenopausal women with early BC in this prospective observational trial...
May 19, 2017: Clinical Breast Cancer
https://www.readbyqxmd.com/read/28600449/regional-differences-in-use-of-endoscopic-ultrasonography-in-ontario-a-population-based-retrospective-cohort-study
#12
Paul D James, Mae Hegagi, Lilia Antonova, Jill Tinmouth, Steven J Heitman, Carmine Simone, Elaine Yeung, Elaine Yong
BACKGROUND: Endoscopic ultrasonography is a safe and accurate modality for evaluating and managing hepatobiliary and gastrointestinal conditions (malignant and nonmalignant); its use is increasing. The aim of this study was to describe regional trends in the use of endoscopic ultrasonography in Ontario. METHODS: We conducted a population-based retrospective cohort study using health administrative databases. We identified all patients who underwent an endoscopic ultrasound procedure in Ontario from 2003 to 2011 using physician billing data...
June 7, 2017: CMAJ Open
https://www.readbyqxmd.com/read/28599790/long-term-outcomes-of-central-neck-dissection-for-cn0-papillary-thyroid-carcinoma
#13
Davide Giordano, Andrea Frasoldati, Enrico Gabrielli, Carmine Pernice, Michele Zini, Andrea Castellucci, Simonetta Piana, Alessia Ciarrocchi, Silvio Cavuto, Verter Barbieri
OBJECTIVE: The risk-benefit ratio of central neck dissection (CND) in patients affected by papillary thyroid carcinoma (PTC) without clinical or ultrasonographic (US) evidence of neck lymph node metastasis (cN0) is currently debated. The aim of this study was to evaluate long-term outcome of CND on locoregional recurrence, distant metastasis, survival, and postoperative complications in a large series of patients with cN0-PTC. STUDY DESIGN: Observational retrospective controlled study...
June 7, 2017: American Journal of Otolaryngology
https://www.readbyqxmd.com/read/28597898/maxwell-stefan-diffusion-coefficient-estimation-for-ternary-systems-an-ideal-ternary-alcohol-system
#14
Tariq Allie-Ebrahim, Qingyu Zhu, Pierre Bräuer, Geoff D Moggridge, Carmine D'Agostino
The Maxwell-Stefan model is a popular diffusion model originally developed to model diffusion of gases, which can be considered thermodynamically ideal mixtures, although its application has been extended to model diffusion in non-ideal liquid mixtures as well. A drawback of the model is that it requires the Maxwell-Stefan diffusion coefficients, which are not based on measurable quantities but they have to be estimated. As a result, numerous estimation methods, such as the Darken model, have been proposed to estimate these diffusion coefficients...
June 21, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28589226/the-first-acidobacterial-laccase-like-multicopper-oxidase-revealed-by-metagenomics-shows-high-salt-and-thermo-tolerance
#15
Luka Ausec, Francesca Berini, Carmine Casciello, Mariana Silvia Cretoiu, Jan Dirk van Elsas, Flavia Marinelli, Ines Mandic-Mulec
Metagenomics is a powerful tool that allows identifying enzymes with novel properties from the unculturable component of microbiomes. However, thus far only a limited number of laccase or laccase -like enzymes identified through metagenomics has been subsequently biochemically characterized. This work describes the successful bio-mining of bacterial laccase-like enzymes in an acidic bog soil metagenome and the characterization of the first acidobacterial laccase-like multicopper oxidase (LMCO). LMCOs have hitherto been mostly studied in fungi and some have already found applications in diverse industries...
June 6, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28587250/removal-of-carmine-from-aqueous-solution-by-carbonated-hydroxyapatite-nanorods
#16
Guanxiong Liu, Caibao Xue, Peizhi Zhu
In this study, carbonated hydroxyapatite (CHA) nanorods were prepared by a novel hydrothermal method. The crystallinity and chemical structure of synthesized CHA nanorods was characterized by transmission electron microscopy (TEM), X-ray diffraction (XRD), Raman spectroscopy and X-ray photoelectron spectroscopy (XPS), respectively. Carmine was selected as representative organic dyes to study the adsorption capacities of CHA nanorods. Mechanistic studies of carmine adsorption by CHA nanorods show that the adsorption processes both follow the pseudo-second-order kinetic model and fit the Langmuir isotherm model well...
June 5, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28578125/efficient-fluorescence-detection-of-protoporphyrin-ix-in-metastatic-lymph-nodes-of-murine-colorectal-cancer-stained-with-indigo-carmine
#17
Hisataka Matsuo, Yoshinori Harada, Takeo Minamikawa, Yoshiyuki Kato, Yasutoshi Murayama, Eigo Otsuji, Tetsuro Takamatsu, Hideo Tanaka
Protoporphyrin IX (PpIX), a biochemical converted from 5-aminolevulinc acid (5-ALA) in living cells, is useful for intraoperative fluorescent detection of cancer metastasis in lymph nodes (LNs). However, unknown is whether the fluorescence of PpIX can be detected in the LNs when they coexist with indigo carmine, a blue dye commonly used for identification of sentinel LNs during surgery. To address this issue, we sought to evaluate the diagnostic usefulness of PpIX fluorescence in the presence of indigo carmine in a mouse LN metastasis model of rectal cancer after administration of 5-ALA...
May 31, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28577414/dynamic-measurement-of-speed-of-sound-in-n-heptane-by-ultrasonics-during-fuel-injections
#18
Elisa Minnetti, Giuseppe Pandarese, Piersavio Evangelisti, Francisco Rodriguez Verdugo, Carmine Ungaro, Alessandro Bastari, Nicola Paone
The paper presents a technique to measure the speed of sound in fuels based on pulse-echo ultrasound. The method is applied inside the test chamber of a Zeuch-type instrument used for indirect measurement of the injection rate (Mexus). The paper outlines the pulse-echo method, considering probe installation, ultrasound beam propagation inside the test chamber, typical signals obtained, as well as different processing algorithms. The method is validated in static conditions by comparing the experimental results to the NIST database both for water and n-Heptane...
May 22, 2017: Ultrasonics
https://www.readbyqxmd.com/read/28574450/stable-abts-immobilized-in-the-mil-100-fe-metal-organic-framework-as-an-efficient-mediator-for-laccase-catalyzed-decolorization
#19
Youxun Liu, Yuanyuan Geng, Mingyang Yan, Juan Huang
The successful encapsulation of 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), a well-known laccase mediator, within a mesoporous metal-organic framework sample (i.e., MIL-100(Fe)) was achieved using a one-pot hydrothermal synthetic method. The as-prepared ABTS@MIL-100(Fe) was characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transform infrared (FT-IR) spectroscopy, nitrogen sorption, and cyclic voltammetry (CV). Our ABTS@MIL-100(Fe)-based electrode exhibited an excellent electrochemical response, indicating that MIL-100(Fe) provides an appropriate microenvironment for the immobilization and electroactivity of ABTS molecules...
June 2, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28572119/right-ventricular-dyssynchrony-and-exercise-capacity-in-idiopathic-pulmonary-arterial-hypertension
#20
Roberto Badagliacca, Silvia Papa, Gabriele Valli, Beatrice Pezzuto, Roberto Poscia, Manuela Reali, Giovanna Manzi, Elisa Giannetta, Daniele Berardi, Susanna Sciomer, Paolo Palange, Francesco Fedele, Robert Naeije, Carmine Dario Vizza
Survival in patients with pulmonary arterial hypertension (PAH) is determined by right ventricular (RV) function adaptation to afterload. How altered RV function impacts on exercise capacity in PAH is not exactly known.104 idiopathic PAH (IPAH) patients aged 52±14 years underwent a diagnostic right heart catheterisation, a comprehensive echocardiography including two-dimensional speckle tracking for RV dyssynchrony evaluation and a cardiopulmonary exercise test. Multivariate analyses were performed to identify independent predictors of peak oxygen uptake (peak V'O2 )...
June 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
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