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https://www.readbyqxmd.com/read/29447580/hplc-pda-determination-of-carminic-acid-and-4-aminocarminic-acid-using-relative-molar-sensitivities-with-respect-to-caffeine
#1
Yuzo Nishizaki, Naoko Sato-Masumoto, Aki Yokota, Tsuyoshi Mikawa, Koichi Nakashima, Taichi Yamazaki, Miho Kuroe, Masahiko Numata, Toshihide Ihara, Yusai Ito, Naoki Sugimoto, Kyoko Sato
To accurately determine carminic acid (CA) and its derivative 4-aminocarminic acid (4-ACA), a novel, high-performance liquid chromatography with photodiode array detector (HPLC/PDA) method using relative molar sensitivity (RMS) was developed. The method requires no analytical standards of CA and 4-ACA; instead it uses the RMS values with respect to caffeine (CAF), which is used as an internal standard. An off-line combination of 1 H-quantitative nuclear magnetic resonance spectroscopy ( 1 H-qNMR) and HPLC/PDA was able to precisely determine the RMSs of CA 274nm /CAF 274nm and 4-ACA 274nm /CAF 274nm ...
February 15, 2018: Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment
https://www.readbyqxmd.com/read/29439278/delineation-of-the-extent-of-early-gastric-cancer-by-magnifying-narrow-band-imaging-and-chromoendoscopy-a-multicenter-randomized-controlled-trial
#2
Takashi Nagahama, Kenshi Yao, Noriya Uedo, Hisashi Doyama, Tetsuya Ueo, Kunihisa Uchita, Hideki Ishikawa, Takashi Kanesaka, Yasuhito Takeda, Kurato Wada, Kentaro Imamura, Hisatomi Arima, Toshio Shimokawa
BACKGROUND: Accurate delineation of tumor margins is necessary for curative resection of early gastric cancer (EGC). The objective of this multicenter, randomized, controlled study was to compare the accuracy with which magnifying narrow-band imaging (M-NBI) and indigo carmine chromoendoscopy delineate EGC margins. METHODS: Patients with EGC ≥ 10 mm undergoing endoscopic or surgical resection were enrolled. The oral-side margins of the lesions were first evaluated with conventional white-light endoscopy in both groups and then delineated by either chromoendoscopy or M-NBI...
February 13, 2018: Endoscopy
https://www.readbyqxmd.com/read/29437994/metabolic-profiling-of-visceral-adipose-tissue-from-obese-subjects-with-or-without-metabolic-syndrome
#3
Eleonora Candi, Manfredi Tesauro, Carmine Cardillo, Anna Maria Lena, Francesca Schinzari, Giuseppe Rodia, Giuseppe Sica, Paolo Gentileschi, Valentina Rovella, Margherita Annicchiarico-Petruzzelli, Nicola Di Daniele, Gerry Melino
Obesity represents one of the most complex public health challenges and has recently reached epidemic proportions.  Obesity is also considered to be primarily responsible for the rising prevalence of metabolic syndrome, defined as the coexistence in the same individual of several risk factors for atherosclerosis, including dyslipidaemia, hypertension and hyperglycaemia, as well as for cancer. Additionally, the presence of three of the five risk factors (abdominal obesity, low HDL cholesterol, high triglycerides, high fasting glucose and high blood pressure) characterizes metabolic syndrome, which has serious clinical consequences...
February 8, 2018: Biochemical Journal
https://www.readbyqxmd.com/read/29433905/quality-of-life-compliance-safety-and-effectiveness-in-fit-older-metastatic-colorectal-patients-with-cancer-treated-in-first-line-with-chemotherapy-plus-cetuximab-a-restrospective-analysis-from-the-observer-study
#4
Gerardo Rosati, Carmine Pinto, Francesca Di Fabio, Silvana Chiara, Ivan R Lolli, Enzo M Ruggeri, Libero Ciuffreda, Raimondo Ferrara, Lorenzo Antonuzzo, Daniela Adua, Patrizia Racca, Domenico Bilancia, Elena Benincasa, Maria Elena Stroppolo, Francesco Di Costanzo
OBJECTIVES: The influence of age (<70 years and ≥70 years) was retrospectively studied on the quality of life (QoL), incidence of side effects (including skin reactions) and efficacy of chemotherapy plus cetuximab in patients with KRAS wild type (WT) metastatic colorectal cancer (mCRC). METHODS: 225 patients of the Observed study (PS 0-1) were retrieved based on age (< 70 and ≥70 years) and evaluated through EORTC QLQ-C30 and DLQI questionnaires...
February 9, 2018: Journal of Geriatric Oncology
https://www.readbyqxmd.com/read/29433190/peptidome-profiles-and-bioactivity-elucidation-of-buffalo-milk-dairy-products-after-gastrointestinal-digestion
#5
Manuela Giovanna Basilicata, Giacomo Pepe, Eduardo Sommella, Carmine Ostacolo, Michele Manfra, Gennaro Sosto, Giuseppe Pagano, Ettore Novellino, Pietro Campiglia
Buffalo milk is highly appreciated for its nutritive properties and highly employed in dairy products, despite this the release of bioactive peptides has not been investigated thoroughly. The aim of this work was to characterize in detail the bioaccesible peptides from buffalo-milk dairy products. Six products were subjected to in vitro simulated gastrointestinal digestion and then analyzed by LC-HRMS. The identified peptides were 165 in Yoghurt, 152 in Scamorza, 146 in Mozzarella, 136 in Grana and Ricotta, 120 in Ice Cream samples, belonging to both buffalo caseins (αs1-, β-, k-CN) and whey proteins (α-LA, β-LG)...
March 2018: Food Research International
https://www.readbyqxmd.com/read/29428835/epicardial-pacing-wire-migration-into-the-thoracic-aorta
#6
Pietro G Malvindi, Vito Margari, Antonella Favale, Georgios Kounakis, Giuseppe Visicchio, Domenico Paparella, Carmine Carbone
No abstract text is available yet for this article.
February 8, 2018: Annals of Thoracic Surgery
https://www.readbyqxmd.com/read/29427340/influence-of-the-feed-pipe-position-of-an-industrial-scale-two-phase-decanter-on-extraction-efficiency-and-chemical-sensory-characteristics-of-virgin-olive-oil
#7
Francesco Caponio, Giacomo Squeo, Lucio Brunetti, Antonella Pasqualone, Carmine Summo, Vito M Paradiso, Pasquale Catalano, Biagio Bianchi
BACKGROUND: Nowadays, olive oil extraction is basically made by means of two-phase decanters, which allow to reduce water consumption and leaching of phenolic compounds. Despite this, most of the working settings derive from studies carried out on three-phase decanters. Hence, aim of the present study has been assessing the influence of two-phase decanter feed pipe position (FP) on the extraction efficiency and chemical-sensory characteristics of virgin olive oil. Three different positions have been considered, at 825 mm (FP1), 610 mm (FP2), and 520 mm (FP3) from the outlet of the oily phase...
February 10, 2018: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/29423057/interleukin-34-sustains-pro-tumorigenic-signals-in-colon-cancer-tissue
#8
Eleonora Franzè, Vicenzo Dinallo, Angela Rizzo, Martina Di Giovangiulio, Gerolamo Bevivino, Carmine Stolfi, Flavio Caprioli, Alfredo Colantoni, Angela Ortenzi, Antonio Di Grazia, Giuseppe Sica, Pier Paolo Sileri, Piero Rossi, Giovanni Monteleone
Interleukin-34 (IL-34), a cytokine produced by a wide range of cells, binds to the macrophage colony-stimulating factor receptor (M-CSFR-1) and receptor-type protein-tyrosine phosphatase zeta (PTP-z) and controls myeloid cell differentiation, proliferation and survival. various types of cancers over-express IL-34 but the role of the cytokine in colorectal cancer (CRC) remains unknown. We here investigated the expression and functional role of IL-34 in CRC. A more pronounced expression of IL-34 was seen in CRC samples as compared to matched normal/benign colonic samples and this occurred at both RNA and protein level...
January 9, 2018: Oncotarget
https://www.readbyqxmd.com/read/29422253/robotic-versus-laparoscopic-radical-hysterectomy-in-early-cervical-cancer-a-case-matched-control-study
#9
Valerio Gallotta, Carmine Conte, Alex Federico, Giuseppe Vizzielli, Salvatore Gueli Alletti, Lucia Tortorella, Luigi Pedone Anchora, Francesco Cosentino, Vito Chiantera, Anna Fagotti, Marco D'Indinosante, Silvia Pelligra, Giovanni Scambia, Gabriella Ferrandina
BACKGROUND: This study aims at evaluating the feasibility, surgical outcome and oncological results observed after robotic radical hysterectomy (RH) compared to laparoscopy for patients with early stage cervical cancer (ECC) patients. METHODS: Between January 2010 and October 2016, 210 patients underwent RH for treatment of ECC: 70 underwent robotic approach (Cases), and 140 underwent laparoscopic approach (Controls). RESULTS: There was no statistically significant difference between the two approaches with regard to clinical patient characteristics and in terms of extent of RH and rate of pelvic and aortic lymphadenectomy...
February 5, 2018: European Journal of Surgical Oncology
https://www.readbyqxmd.com/read/29421998/the-role-of-selenium-in-oxidative-stress-and-in-nonthyroidal-illness-syndrome-ntis-an-overview
#10
Andrea Silvestrini, Alvaro Mordente, Giuseppe Martino, Carmine Bruno, Edoardo Vergani, Elisabetta Meucci, Antonio Mancini
Selenium is a trace element, nutritionally classified as essential micronutrient, involved in maintaining the correct function of several enzymes incorporating the selenocysteine residue, namely the selenoproteins. The human selenoproteome including 25 proteins is here extensively described. The most relevant selenoproteins, including glutathione peroxidases, thioredoxin reductases and iodothyronine deiodinases are required for the proper cellular redox homeostasis as well as for the correct thyroid function, thus preventing oxidative stress and related diseases...
January 31, 2018: Current Medicinal Chemistry
https://www.readbyqxmd.com/read/29421793/phytotherapy-in-functional-gastrointestinal-disorders
#11
Olaf Kelber, Rudolf Bauer, Wolfgang Kubelka
BACKGROUND: Phytotherapy is an important therapeutic option in functional gastrointestinal diseases (FGID). It has a large tradition, with different approaches in different regions of the world, some of which have made their way into modern evidence-based medicine (EBM). SUMMARY: Taking into account the number of herbs in use, and also the cumulated scientific evidence on them, FGID are possibly the most important indication in phytotherapy. This does not only apply for European phytotherapy, but also for other regions, such as Asia...
February 8, 2018: Digestive Diseases
https://www.readbyqxmd.com/read/29420792/assessment-of-neurological-toxicity-of-hydroxyethyl-starch-130-0-4-injected-in-the-intrathecal-space-in-rats
#12
O Vassal, P Del Carmine, F-P Desgranges, L Bouvet, M Lilot, N Gadot, Q Timour-Chah, D Chassard
Objective: Epidural blood patch is the procedure of choice to relieve postdural puncture headache. Hydroxyethyl-starch (HES) has been proposed as a patch in some circumstances such as in the case of hematological disease due to the theoretical risk of neoplastic seeding to the central nervous system. Acute neurological HES toxicity has been excluded by a previous animal study, but the long-term neurological toxicity has not been evaluated. Methods: Rats were randomly assigned to one of three groups: no intrathecal injection, 20 μL of intrathecal saline, or a 20-μL intrathecal HES (6% hydroxyethyl starch 130/0...
February 6, 2018: Pain Medicine: the Official Journal of the American Academy of Pain Medicine
https://www.readbyqxmd.com/read/29416701/exploring-the-molecular-aspects-associated-with-testicular-germ-cell-tumors-a-review
#13
REVIEW
Gaetano Facchini, Sabrina Rossetti, Carla Cavaliere, Carmine D'Aniello, Rossella Di Franco, Gelsomina Iovane, Giovanni Grimaldi, Raffaele Piscitelli, Paolo Muto, Gerardo Botti, Sisto Perdonà, Bianca Maria Veneziani, Massimiliano Berretta, Micaela Montanari
Testicular germ cell tumors (TGCTs) represent the most common solid tumors affecting young men. They constitute a distinct entity because of their embryonic origin and their unique biological behavior. Recent preclinical data regarding biological signaling machinery as well as genetic and epigenetic mechanisms associated with molecular patterns of tumors have contribute to explain the pathogenesis and the differentiation of TGCTs and to understand the mechanisms responsible for the development of resistance to treatment...
January 2, 2018: Oncotarget
https://www.readbyqxmd.com/read/29415476/the-impact-of-aging-on-cardio-and-cerebrovascular-diseases
#14
REVIEW
Carmine Izzo, Albino Carrizzo, Antonia Alfano, Nicola Virtuoso, Mario Capunzo, Mariaconsiglia Calabrese, Eros De Simone, Sebastiano Sciarretta, Giacomo Frati, Marco Oliveti, Antonio Damato, Mariateresa Ambrosio, Francesco De Caro, Paolo Remondelli, Carmine Vecchione
A growing number of evidences report that aging represents the major risk factor for the development of cardio and cerebrovascular diseases. Understanding Aging from a genetic, biochemical and physiological point of view could be helpful to design a better medical approach and to elaborate the best therapeutic strategy to adopt, without neglecting all the risk factors associated with advanced age. Of course, the better way should always be understanding risk-to-benefit ratio, maintenance of independence and reduction of symptoms...
February 6, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29399612/validity-of-conventional-endoscopy-using-non-extension-sign-for-optical-diagnosis-of-colorectal-deep-submucosal-invasive-cancer
#15
Takashi Hisabe, Sumio Tsuda, Toshio Hoashi, Hiroshi Ishihara, Kazutomo Yamasaki, Tatsuhisa Yasaka, Fumihito Hirai, Toshiyuki Matsui, Kenshi Yao, Hiroshi Tanabe, Akinori Iwashita
Background and study aims:  The non-extension sign relates to a localized increase in thickness and rigidity due to deep submucosal invasive (SM-d: depth of 1000 μm or more) cancer. The present study aimed to evaluate the efficacy of the non-extension sign in assessing the optical diagnosis of colorectal SM-d cancer. Patients and methods:  We retrospectively analyzed 309 patients with 315 early colorectal cancers that had been endoscopically or surgically resected...
February 2018: Endoscopy International Open
https://www.readbyqxmd.com/read/29399336/cardiovascular-characteristics-of-chronic-fatigue-syndrome
#16
Sara Bozzini, Andrea Albergati, Enrica Capelli, Lorenzo Lorusso, Carmine Gazzaruso, Gabriele Pelissero, Colomba Falcone
Patients with chronic fatigue syndrome (CFS) commonly exhibit orthostatic intolerance. Abnormal sympathetic predominance in the autonomic cardiovascular response to gravitational stimuli was previously described in numerous studies. The aim of the current study was to describe cardiological and clinical characteristics of Italian patients with CFS. All of the patients were of Caucasian ethnicity and had been referred to our center, the Cardiology Department of the University Hospital of Pavia (Pavia, Italy) with suspected CFS...
January 2018: Biomedical Reports
https://www.readbyqxmd.com/read/29390909/colpocytological-abnormalities-in-hiv-infected-and-uninfected-pregnant-women-prevalence-persistence-and-progression
#17
Carmine Carriero, Fabiana Divina Fascilla, Paola Cramarossa, Achiropita Lepera, Stefano Bettocchi, Antonella Vimercati
In this retrospective case-control study, we analyse data of 48 HIV-positive pregnant patients, versus a control group of 99 HIV-negative pregnant women, followed as outpatients by our department from 2009 to 2014. The aims of the study were to investigate the prevalence, persistence and progression of cervical squamous intraepithelial lesions (SIL) in each group and to correlate colpo-cytological lesions to the socio-demographic and clinical-laboratory findings in the HIV + pregnant women. In our study we observed that immunosuppression, HPV infection and vaginal coinfections were predictive of cervical lesions...
February 1, 2018: Journal of Obstetrics and Gynaecology: the Journal of the Institute of Obstetrics and Gynaecology
https://www.readbyqxmd.com/read/29389861/angiopoietin-like-proteins-in-angiogenesis-inflammation-and-cancer
#18
REVIEW
Carmine Carbone, Geny Piro, Valeria Merz, Francesca Simionato, Raffaela Santoro, Camilla Zecchetto, Giampaolo Tortora, Davide Melisi
Altered expression of secreted factors by tumor cells or cells of the tumor microenvironment is a key event in cancer development and progression. In the last decade, emerging evidences supported the autocrine and paracrine activity of the members of the Angiopoietin-like (ANGPTL) protein family in angiogenesis, inflammation and in the regulation of different steps of carcinogenesis and metastasis development. Thus, ANGPTL proteins become attractive either as prognostic or predictive biomarkers, or as novel target for cancer treatment...
February 1, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29389566/a-novel-poly-glycine-biosensor-towards-the-detection-of-indigo-carmine-a-voltammetric-study
#19
Jamballi Gangadharappa Gowda Manjunatha
The electrochemical behavior of indigo carmine (IC) at poly (glycine) modified carbon paste electrode (PGMCPE) was investigated by cyclic and differential pulse voltammetry. The oxidation peak of IC was observed in phosphate buffer of pH 6.5. The influence of different pH, scan rate, and concentration were analyzed. The probable reaction mechanism involved in the oxidation of IC was also proposed. Results showed that PGMCPE a remarkable electrocatalytic activity for the oxidation of IC under optimal conditions...
January 2018: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/29386141/analysis-of-bluetongue-serotype-3-spread-in-tunisia-and-discovery-of-a-novel-strain-related-to-the-bluetongue-virus-isolated-from-a-commercial-sheep-pox-vaccine
#20
Alessio Lorusso, Soufien Sghaier, Marco Di Domenico, Mohamed Elias Barbria, Guendalina Zaccaria, Aida Megdich, Ottavio Portanti, Imed Ben Seliman, Massimo Spedicato, Federica Pizzurro, Irene Carmine, Liana Teodori, Mejdi Mahjoub, Iolanda Mangone, Alessandra Leone, Salah Hammami, Maurilia Marcacci, Giovanni Savini
Bluetongue (BT), is one of the OIE-listed major diseases of ruminants. Following the official report of BT virus serotype 3 (BTV-3) in a sheep in Cap Bon (Tunisia), blood and serum samples of ruminants were collected from some areas of Tunisia to further investigate the presence of this virus in the country. To this aim, a specific quantitative real time RT-PCR has been first developed for the detection and quantitation of BTV-3 RNA from field specimens. Out of 62 collected blood samples, 23 were shown to be positive for BTV-3 RNA...
January 28, 2018: Infection, Genetics and Evolution
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