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https://www.readbyqxmd.com/read/28829890/mir-367-promotes-uveal-melanoma-cell-proliferation-and-migration-by-regulating-pten
#1
J W Ling, P R Lu, Y B Zhang, S Jiang, Z C Zhang
We aimed to investigate the biological role of miR-367 in uveal melanoma cell growth and migration, and the underlying mechanism responsible. Quantitative real-time polymerase chain reaction was performed to evaluate miR-367 expression in uveal melanoma tissue samples and cell lines. A miR-367 mimic, miR-367 inhibitor, and negative control oligonucleotide were transfected into these cells to investigate the function of this microRNA. In addition, the role of PTEN in miR-367-mediated uveal melanoma cell growth and migration was evaluated...
August 17, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28829888/mir-200c-inhibits-metastasis-of-breast-tumor-via-the-downregulation-of-foxf2
#2
T Zhang, J G Wan, J B Liu, M Deng
The forkhead box F2 (Foxf2) gene suppresses epithelial-mesenchymal transition via the modulation of transcription of zinc finger E-box-binding homeobox 1 (Zeb1) and epithelial (E)-cadherin, thereby inhibiting tumor metastasis. Additionally, the specific binding of microRNA (miR)-200c to Foxf2 mRNA impedes metastatic pulmonary cancer. However, the role of miR-200c in breast cancer is still unknown. Therefore, in this study, miR-200c mimics were transfected into the highly metastatic breast cancer cell line MDA-MB-231...
August 17, 2017: Genetics and Molecular Research: GMR
https://www.readbyqxmd.com/read/28829800/identification-of-protoplast-isolation-responsive-micrornas-in-citrus-reticulata-blanco-by-high-throughput-sequencing
#3
Xiaoyong Xu, Xiaoling Xu, Yipeng Zhou, Shaohua Zeng, Weiwen Kong
Protoplast isolation is a stress-inducing process, during which a variety of physiological and molecular alterations take place. Such stress response affects the expression of totipotency of cultured protoplasts. MicroRNAs (miRNAs) play important roles in plant growth, development and stress responses. However, the underlying mechanism of miRNAs involved in the protoplast totipotency remains unclear. In this study, high-throughput sequencing technology was used to sequence two populations of small RNA from calli and callus-derived protoplasts in Citrus reticulata Blanco...
2017: PloS One
https://www.readbyqxmd.com/read/28829792/effect-of-endurance-exercise-on-micrornas-in-myositis-skeletal-muscle-a-randomized-controlled-study
#4
Jessica F Boehler, Marshall W Hogarth, Matthew D Barberio, James S Novak, Svetlana Ghimbovschi, Kristy J Brown, Li Alemo Munters, Ingela Loell, Yi-Wen Chen, Heather Gordish-Dressman, Helene Alexanderson, Ingrid E Lundberg, Kanneboyina Nagaraju
OBJECTIVE: To identify changes in skeletal muscle microRNA expression after endurance exercise and associate the identified microRNAs with mRNA and protein expression to disease-specific pathways in polymyositis (PM) and dermatomyositis (DM) patients. METHODS: Following a parallel clinical trial design, patients with probable PM or DM, exercising less than once a week, and on stable medication for at least one month were randomized into two groups at Karolinska University Hospital: a 12-week endurance exercise group (n = 12) or a non-exercised control group (n = 11)...
2017: PloS One
https://www.readbyqxmd.com/read/28829698/the-pro-%C3%AE-1-v-collagen-gene-col5a1-is-coordinately-regulated-by-mir-29b-with-core-promoter-in-cultured-cells
#5
Juan Juan Zhang, Hiroyuki Yano, Takako Sasaki, Noritaka Matsuo, Hidekatsu Yoshioka
AIMS: Col5a1 encodes the α1 chain of type V collagen, a quantitatively minor fibrillar collagen that is critical for the formation and function of the organs in the body. MicroRNAs (miRNAs) are small noncoding RNAs that post-transcriptionally regulate biological functions by binding to the 3'- untranslated region (3'UTR) of specific target mRNA. In this study, we investigated the post-transcriptional regulation of miRNAs on the Col5a1 gene expression. MATERIALS AND METHODS: We cultured osteoblasts and fibroblasts of cell lines...
August 22, 2017: Connective Tissue Research
https://www.readbyqxmd.com/read/28829507/microrna-613-attenuates-the-proliferation-migration-and-invasion-of-wilms-tumor-via-targeting-frs2
#6
H-F Wang, Y-Y Zhang, H-W Zhuang, M Xu
OBJECTIVE: Wilms' tumor is the most common malignant tumor in children worldwide. Considering the poor therapeutic effect on Wilms' tumor, we determined the effects of microRNA-613 on cell proliferation and metastasis in vitro, providing therapeutic targets for the treatment of Wilms' tumor. PATIENTS AND METHODS: Quantitative real-time PCR (qRT-PCR) was employed to identify the expression level of miR-613. CCK8 and colony formation assays were incorporated to assess cell viability and proliferation capacity...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829506/effects-of-mir-455-on-pik3r1-gene-expression-regulation-and-kidney-cancer-cell-functions
#7
Y-D Wang, Z-L Sun
OBJECTIVE: Migration and proliferation of kidney cancer cells are critical factors affecting effective treatment. Abnormal gene expression exists during cancer pathogenesis. The study showed certain effects of PIK3R1 gene on migration and proliferation of kidney cancer cells, although a few studies have been performed regulatory mechanism of microRNA on PIK3R1 gene. Previous sequencing of PIK3R1 gene found the existence of mir-455 binding site. This study confirmed the regulation of PIK3R1 gene expression in kidney cancer cells of mir-455 by in vitro assay, and analyzed its effects on migration or proliferation of kidney cancer cells...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829505/dnmt3a-mediated-down-regulation-of-microrna-105-promotes-gastric-cancer-cell-proliferation
#8
G-Q Zhou, F Han, Z-L Shi, L Yu, X-F Li, C Yu, C-L Shen, D-W Wan, X-G Zhu, R Li, S-B He
OBJECTIVE: It has been well-established that microRNAs (miRNAs), a class of short non-coding RNA molecules, play an important role in the development of gastric cancer. In the present study, we focused on miR-105, a novel miRNA not previously linked to gastric cancer. PATIENTS AND METHODS: 36 paired surgically resected gastric cancer tissues and matched adjacent normal tissues were used to detect the expression of miR-105. AGS cells were used to overexpress or silence of miR-105 and to determine its effect on several tumorigenic properties...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829503/microrna-124-inhibits-cell-invasion-and-epithelial-mesenchymal-transition-by-directly-repressing-snail2-in-gastric-cancer
#9
S-L Li, H-L Gao, X-K Lv, Y-R Hei, P-Z Li, J-X Zhang, N Lu
OBJECTIVE: MicroRNAs (miRNAs) act as critical regulators of genes expression involved in tumor biological processes. The study aimed to investigate the clinical significance and biological role of miR-124 in gastric cancer (GC). PATIENTS AND METHODS: MiR-124 expression was analyzed from 88 GC tissues and adjacent normal tissues by quantitative Real-time PCR (qRT-PCR). Kaplan-Meier curves and log-rank test was used to evaluate the association between miR-124 and the over survival (OS) time of GC patients...
August 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28829439/an-integrated-expression-atlas-of-mirnas-and-their-promoters-in-human-and-mouse
#10
Derek de Rie, Imad Abugessaisa, Tanvir Alam, Erik Arner, Peter Arner, Haitham Ashoor, Gaby Åström, Magda Babina, Nicolas Bertin, A Maxwell Burroughs, Ailsa J Carlisle, Carsten O Daub, Michael Detmar, Ruslan Deviatiiarov, Alexandre Fort, Claudia Gebhard, Daniel Goldowitz, Sven Guhl, Thomas J Ha, Jayson Harshbarger, Akira Hasegawa, Kosuke Hashimoto, Meenhard Herlyn, Peter Heutink, Kelly J Hitchens, Chung Chau Hon, Edward Huang, Yuri Ishizu, Chieko Kai, Takeya Kasukawa, Peter Klinken, Timo Lassmann, Charles-Henri Lecellier, Weonju Lee, Marina Lizio, Vsevolod Makeev, Anthony Mathelier, Yulia A Medvedeva, Niklas Mejhert, Christopher J Mungall, Shohei Noma, Mitsuhiro Ohshima, Mariko Okada-Hatakeyama, Helena Persson, Patrizia Rizzu, Filip Roudnicky, Pål Sætrom, Hiroki Sato, Jessica Severin, Jay W Shin, Rolf K Swoboda, Hiroshi Tarui, Hiroo Toyoda, Kristoffer Vitting-Seerup, Louise Winteringham, Yoko Yamaguchi, Kayoko Yasuzawa, Misako Yoneda, Noriko Yumoto, Susan Zabierowski, Peter G Zhang, Christine A Wells, Kim M Summers, Hideya Kawaji, Albin Sandelin, Michael Rehli, Yoshihide Hayashizaki, Piero Carninci, Alistair R R Forrest, Michiel J L de Hoon
MicroRNAs (miRNAs) are short non-coding RNAs with key roles in cellular regulation. As part of the fifth edition of the Functional Annotation of Mammalian Genome (FANTOM5) project, we created an integrated expression atlas of miRNAs and their promoters by deep-sequencing 492 short RNA (sRNA) libraries, with matching Cap Analysis Gene Expression (CAGE) data, from 396 human and 47 mouse RNA samples. Promoters were identified for 1,357 human and 804 mouse miRNAs and showed strong sequence conservation between species...
August 21, 2017: Nature Biotechnology
https://www.readbyqxmd.com/read/28829370/mir-30a-5p-inhibits-epithelial-to-mesenchymal-transition-and-upregulates-expression-of-tight-junction-protein-claudin-5-in-human-upper-tract-urothelial-carcinoma-cells
#11
Yueh-Hua Chung, Sung-Chou Li, Ying-Hsien Kao, Hao-Lun Luo, Yuan-Tso Cheng, Pey-Ru Lin, Ming-Hong Tai, Po-Hui Chiang
The involvement of microRNAs (miRNAs) in cancer development and their potential as prognostic biomarkers are becoming increasingly known. However, the signature of miRNAs and their regulatory roles in tumorigenesis of upper tract urothelial carcinoma (UTUC) remain to be elucidated. This study aimed to profile the miRNA expression pattern in UTUC tumor tissues and identify candidate miRNAs with prognostic and/or therapeutic functions. METHODS AND RESULTS: We collected 22 UTUC tissue and adjacent normal tissues samples from patients who underwent nephroureterectomy...
August 22, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28829278/glucose-6-phosphatase-%C3%AE-participates-in-dopaminergic-differentiation
#12
Jing Chen, Ting Wang, Xiao-Yan Zhou, Chuan-Xi Tang, Dian-Shuai Gao
OBJECTIVE:  Induction of dopaminergic (DA) differentiation is a cell-based therapy for Parkinson's disease (PD). Here, we explore the key factors of DA differentiation with a focus on glucose-6-phosphatase (G6Pase), a marker enzyme for the endoplasmic reticulum (ER) associated with cell differentiation. METHODS:  We cultured SH-SY5Y human neuroblastoma cells, a model system for PD research, and added glial cell-derived neurotrophic factor (GDNF; 25, 50, or 100 ng/ml) to stimulate differentiation...
August 22, 2017: Neurological Research
https://www.readbyqxmd.com/read/28829207/invasive-candidiasis-future-directions-in-non-culture-based-diagnosis
#13
Wilfried Posch, David Heimdörfer, Doris Wilflingseder, Cornelia Lass-Flörl
Delayed initial antifungal therapy is associated with high mortality rates caused by invasive Candida infections, since accurate detection of the opportunistic pathogenic yeast and its identification display a diagnostic challenge. Diagnosis of Candida infections relies on time-consuming methods such as blood cultures, serologic and histopathologic examination. To allow for fast detection and characterization of invasive candidiasis, there is a need to improve diagnostic tools. Trends in diagnostics switch to non-culture-based methods, which allow specified diagnosis within significantly shorter periods of time in order to provide early and appropriate antifungal treatment...
August 22, 2017: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/28828971/molecular-biomarkers-of-anaplastic-thyroid-carcinoma
#14
F Bozorg-Ghalati, M Hedayati
Anaplastic thyroid carcinoma is the rarest but extremely aggressive thyroid cancer subtype. This neoplasia is composed of undifferentiated tumor cells with poor prognosis and resistant to common thyroid cancer therapy. Early stage identification of this cancer for prompt treatment is very vital. Presently, cytological evaluation of fine needle aspiration biopsy (FNAB) which is known as invasive recognition assay, is the standard diagnostic method for the diagnosis of malignant thyroid tumors. Frequent studies have suggested that using the molecular biomarkers of thyroid cancer tissue alongside cytological examination, increase the accuracy of diagnostic tests...
August 21, 2017: Current Molecular Medicine
https://www.readbyqxmd.com/read/28828670/isolation-of-extracellular-vesicles-from-breast-milk
#15
Xin Wang
Isolation of extracellular vesicles (EVs) from breast milk enables the downstream analysis of the contents, including various proteins, lipids, and RNAs (messenger RNAs and microRNAs). Here, we present the method of centrifugation with ExoQuick™ precipitation to isolate EVs from breast milk. It has been proven to be a rapid and effective alternative compared to conventional differential centrifugation method.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28828665/extracellular-vesicle-mirna-detection-using-molecular-beacons
#16
Won Jong Rhee, Seunga Jeong
Many studies have recently focused on extracellular vesicles. Extracellular vesicles including exosomes are nanosized vesicles that are produced and secreted by cells. Extracellular vesicles contain various types of nucleic acid biomarkers, including microRNAs (miRNAs ) from their parent cells. As high concentrations of extracellular vesicles are found in most body fluids, miRNA detection of exosomes in body fluids, including blood and urine, enables early and easy disease diagnosis. In this context, the in situ detection method of extracellular vesicles miRNA using molecular beacons is described here...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28828398/poly-adp-ribose-polymerase-1-parp-1-induction-by-cocaine-is-post-transcriptionally-regulated-by-mir-125b
#17
Sabyasachi Dash, Muthukumar Balasubramaniam, Tanu Rana, Arthur Godino, Emily G Peck, Jeffery Shawn Goodwin, Fernando Villalta, Erin S Calipari, Eric J Nestler, Chandravanu Dash, Jui Pandhare
Cocaine exposure alters gene expression in the brain via methylation and acetylation of histones along with methylation of DNA. Recently, poly (ADP-ribose) polymerase-1 (PARP-1) catalyzed PARylation has been reported as an important regulator of cocaine-mediated gene expression. In this study, we report that the cellular microRNA "miR-125b" plays a key role for cocaine-induced PARP-1 expression. Acute and chronic cocaine exposure resulted in the downregulation of miR-125b concurrent with upregulation of PARP-1 in dopaminergic neuronal cells and nucleus accumbens (NAc) of mice but not in the medial prefrontal cortex (PFC) or ventral tegmental area (VTA)...
July 2017: ENeuro
https://www.readbyqxmd.com/read/28828340/cell-free-fetal-nucleic-acid-identifier-markers-in-maternal-circulation
#18
REVIEW
Mahboubeh Ramezanzadeh, Sharifeh Khosravi, Rasoul Salehi
From the discovery of cell-free fetal (cff)-DNA in 1997 so far, many studies have been performed on various aspects of cff-nucleic acid. It is undoubted that currently, invasive prenatal diagnosis progresses to the noninvasive test. However, there are many problems. One of the most challenging issues in this field is differentiation and detection of the small amount of cff-nucleic acid in maternal plasma. Many markers and methods have been used for this purpose. This review makes an attempt to review and compare the studies in the field...
2017: Advanced Biomedical Research
https://www.readbyqxmd.com/read/28828274/micrornas-tend-to-synergistically-control-expression-of-genes-encoding-extensively-expressed-proteins-in-humans
#19
Xue Chen, Wei Zhao, Ye Yuan, Yan Bai, Yong Sun, Wenliang Zhu, Zhimin Du
Considering complicated microRNA (miRNA) biogenesis and action mechanisms, it was thought so high energy-consuming for a cell to afford simultaneous over-expression of many miRNAs. Thus it prompts that an alternative miRNA regulation pattern on protein-encoding genes must exist, which has characteristics of energy-saving and precise protein output. In this study, expression tendency of proteins encoded by miRNAs' target genes was evaluated in human organ scale, followed by quantitative assessment of miRNA synergism...
2017: PeerJ
https://www.readbyqxmd.com/read/28828217/a-pilot-study-on-the-effects-of-probiotic-supplementation-on-neuropsychological-performance-and-microrna-29a-c-levels-in-antiretroviral-treated-hiv-1-infected-patients
#20
Giancarlo Ceccarelli, Mariangela Fratino, Carla Selvaggi, Noemi Giustini, Sara Serafino, Ivan Schietroma, Giuseppe Corano Scheri, Paolo Pavone, Giulia Passavanti, Danilo Alunni Fegatelli, Ivano Mezzaroma, Guido Antonelli, Vincenzo Vullo, Carolina Scagnolari, Gabriella d'Ettorre
INTRODUCTION: The gut microbiota is involved in the regulation of cognition, mood, anxiety, and pain, and can impact cognitive functions by producing neuroactive substances or releasing bacterial by-products and metabolites. No information is available on the effects of a probiotic supplementation on brain function of HIV+ subjects. In light of the above considerations, we performed a pilot study in cART-treated HIV-1-positive patients with long-term virologic suppression. The aims were to analyze the effect of high-concentration multistrain probiotic supplementation (Vivomixx®; Visbiome®) on several neurocognitive abilities and to evaluate the safety of this supplementation...
August 2017: Brain and Behavior
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