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Renqiang Yuan, Xumeng Zhang, Ying Fang, Yaping Nie, Shufang Cai, Yaosheng Chen, Delin Mo
MicroRNAs are a class of highly conserved ∼20 nucleotides non-coding RNAs that post-transcriptionally regulate gene expression. Many miRNAs were studied in the development of skeletal muscle, such as miR-1, miR-206, and miR-133. In our previous study, miR-127-3p was found highly expressed in porcine fetal skeletal muscle, whereas the detailed functions of miR-127-3p in muscle development is still unclear. In this study, we detected that miR-127-3p also highly expressed in skeletal muscle, cardiac muscle of adult mice and proliferative C2C12 cell lines...
June 19, 2018: Biochemical and Biophysical Research Communications
Jian Wu, Jibo Liu, Yaqin Ding, Mingying Zhu, Kan Lu, Jing Zhou, Xin Xie, Yi Xu, Xiaoyu Shen, Yiyue Chen, Xiaohong Shao, Chunling Zhu
Emerging evidence has shown that microRNAs (miRNAs) play a mediatory role in the pathogenesis of diabetic nephropathy (DN), but the function of the involved miRNAs is still incomplete. Here, we found that miR-455-3p was down-regulated in the human mesangial cells (HMC) and human proximal tubule epithelial cells (HK-2) stimulated with high glucose (HG) or transforming growth factor beta 1 (TGF-β1). Rho-associated coiled coil-containing protein kinase 2 (ROCK2) was identified as a directed target of miR-455-3p...
June 19, 2018: Biochemical and Biophysical Research Communications
Longfei Jia, Michael Chopp, Lei Wang, Xuerong Lu, Alexandra Szalad, Zheng Gang Zhang
Schwann cells actively interact with axons of dorsal root ganglia (DRG) neurons. Exosomes mediate intercellular communication by transferring their biomaterials, including microRNAs (miRs) into recipient cells. We hypothesized that exosomes derived from Schwann cells stimulated by high glucose (HG) exosomes accelerate development of diabetic peripheral neuropathy and that exosomal cargo miRs contribute to this process. We found that HG exosomes contained high levels of miR-28, -31a, and -130a compared to exosomes derived from non-HG-stimulated Schwann cells...
June 22, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Shicheng Li, Xiao Sun, Shuncheng Miao, Tong Lu, Yuanyong Wang, Jia Liu, Wenjie Jiao
BACKGROUND: Increasing evidence has demonstrated that circular RNAs (circRNAs) may play an important role in oncogenesis and tumor development; however, their role in lung adenocarcinoma (LUAD) remains unclear. We identified the differentially expressed circRNAs in LUAD and investigated the potential mechanisms for cancer progression. METHODS: We examined differentially expressed circRNAs in LUAD and paired normal tissues using downloaded circRNA microarrays from the Gene Expression Omnibus...
June 22, 2018: Thoracic Cancer
Ahmed A Moustafa, Hogyoung Kim, Rasha S Albeltagy, Ola H El-Habit, Asim B Abdel-Mageed
MicroRNAs (miRNAs) are a small functional non-coding RNAs that post-transcriptionally regulate gene expression through mRNA degradation or translational repression. miRNAs are key regulatory components of various cellular networks. Current evidence support that multiple mammalian genome-encoded miRNAs impact the cellular biology, including proliferation, apoptosis, differentiation, and tumorigenesis, by targeting specific subsets of mRNAs. This minireview is focused on the current themes underlying the interactions between miRNAs and their mRNA targets and pathways in prostate tumorigenesis and progression, and their potential clinical utility as biomarkers for prostate cancer...
June 2018: Experimental Biology and Medicine
Pedro Paulo Gattai, Edgar Maquigussa, Antonio da Silva Novaes, Rosemara da Silva Ribeiro, Vanessa Araújo Varela, Milene Subtil Ormanji, Mirian Aparecida Boim
Acute kidney injury is mostly reversible, and hepatocyte growth factor (HGF) has a relevant role in the tissue repair. MicroRNA (miR)-26a is an endogenous modulator of HGF. The role of miR-26a in the kidney repair process was evaluated in Wistar rats submitted to an acute kidney injury model of rhabdomyolysis induced by glycerol (6 mL/kg). Animals were evaluated 3, 12, 48, 96, and 120 hours after glycerol injection. Serum creatinine (SCr) and gene expression of HGF, c-met, signal transducer and activator of transcription 3 (STAT3), and miR-26a were estimated...
June 22, 2018: Journal of Cellular Biochemistry
Mary Patrice Eastwood, Jan Deprest, Francesca Maria Russo, Hongmei Wang, Drew Mulhall, Barbara Iwasiow, Thomas H Mahood, Richard Keijzer
OBJECTIVE: Profiling of miR-200b expression and its targets (TGF-β2 and ZEB2) in the surgical rabbit CDH model before and after tracheal occlusion (TO). METHODS: Thirty-eight timed-pregnant rabbits had left DH creation on gestational day (GD) 23. On GD28, 17 randomly selected fetuses had TO. We harvested fetuses at GD23, GD28 or GD30. We calculated lung-to-body-weight ratios (LBWR), processing lungs for miR-200b in-situ hybridization (ISH) and RT-qPCR and evaluating effects on downstream targets TGF-β2 or ZEB2...
June 22, 2018: Prenatal Diagnosis
Giulia Maurizi, Lucia Babini, Lucio Della Guardia
Adipose tissue is a dynamic endocrine organ playing a pivotal role in metabolism modulation. Adipocytes differentiation requires a highly orchestrated series of changes of gene expression in precursor cells. At the same time, white mature adipocytes are plastic cells able to reversibly transdifferentiate toward fibroblast-like cells via the liposecretion process, returning back to a non-committed status of the cells. In particular, adipose tissue microenvironment along with external signaling molecules such as adipokines, cytokines and growth factors can regulate adipocytes physiology through complex molecular networks...
June 22, 2018: Journal of Cellular Physiology
Anupam Kumar, Subhashree Nayak, Pankaj Pathak, Suvendu Purkait, Prit Benny Malgulawar, Mehar Chand Sharma, Vaishali Suri, Arijit Mukhopadhyay, Ashish Suri, Chitra Sarkar
INTRODUCTION: Although role of individual microRNAs (miRNAs) in the pathogenesis of gliomas has been well studied, their role as a clustered remains unexplored in gliomas. METHODS: In this study, we performed the expression analysis of miR-379/miR-656 miRNA-cluster (C14MC) in oligodendrogliomas (ODGs) and also investigated the mechanism underlying modulation of this cluster. RESULTS: We identified significant downregulation of majority of the miRNAs from this cluster in ODGs...
June 21, 2018: Journal of Neuro-oncology
Marie-Pierre Lambert, Sophie Terrone, Guillaume Giraud, Clara Benoit-Pilven, David Cluet, Valérie Combaret, Franck Mortreux, Didier Auboeuf, Cyril F Bourgeois
The Repressor Element 1-silencing transcription factor (REST) represses a number of neuronal genes in non-neuronal cells or in undifferentiated neural progenitors. Here, we report that the DEAD box RNA helicase DDX17 controls important REST-related processes that are critical during the early phases of neuronal differentiation. First, DDX17 associates with REST, promotes its binding to the promoter of a subset of REST-targeted genes and co-regulates REST transcriptional repression activity. During neuronal differentiation, we observed a downregulation of DDX17 along with that of the REST complex that contributes to the activation of neuronal genes...
June 21, 2018: Nucleic Acids Research
Zahra Hatefi, Goljahan Soltani, Sharifeh Khosravi, Mohammad Kazemi, Ahmad Reza Salehi, Rasoul Salehi
Background: The relationship between dyslipidemia and type 2 diabetes mellitus (T2DM) has been frequently reported. Lipoprotein lipase (LPL) is considered to be an effective gene in regulating lipid profile. MicroRNAs (miRNAs) are small noncoding RNAs involved in posttranscriptional regulation of gene expression. In the present study, we have evaluated rs13702 (C/T) polymorphism located in miRNA-410 binding site of LPL gene in subset of Iranian T2DM patients and their normal counterparts...
2018: Advanced Biomedical Research
David Weber, Laurence Amar, Daniel Gödde, Christian Prinz
MicroRNAs (miRNAs) are ∼22-nt molecules exerting control of protein expression in cancer tissues. The current study determined the full spectrum of miRNA dysregulation in freshly isolated human colon or rectal cancer biopsies as well as in controls of healthy adjacent tissue (total of n = 100) using an Illumina sequencing technology. In this work, we aimed to identify miRNAs that may serve as future marker to discern between these two subtypes. DESeq2 analysis revealed 53 significantly dysregulated miRNAs in colon cancer, 67 miRNAs in rectal cancer, and 97 miRNAs in both at a P adj value < 0...
June 5, 2018: Oncotarget
Xudong Huang, Jian Zheng, Jun Li, Xu Che, Wenle Tan, Wen Tan, Mingming Shao, Xinxin Cheng, Zhongli Du, Yanjie Zhao, Chengfeng Wang, Chen Wu, Dongxin Lin
Genetic variation (rs372883C/T) in the 3'-untranslated region of BTB and CNC homology 1 ( BACH1 ) has been associated with pancreatic ductal adenocarcinoma (PDAC) risk in our previous genome-wide association study; however, the action roles of this genetic variation in PDAC remains unknown. Methods: BACH1 expression was measured by quantitative real-time PCR, Western blot and immunohistochemistry. The effects of BACH1 on cell proliferation and sensitivity to gemcitabine were examined by alteration of BACH1 expression in PDAC cells...
2018: Theranostics
Ping Zhu, Nan Ge, Dongyan Liu, Fan Yang, Kai Zhang, Jintao Guo, Xiang Liu, Sheng Wang, Guoxin Wang, Siyu Sun
The incidence of pancreatic cancer is increasing annually in Asia as a whole. Pancreatic cancer ranks sixth in terms of incidence of all malignant tumors. Circular RNA (circRNA) is a type of non-coding RNA which forms a covalently closed continuous loop. CircRNA is extensively expressed in the cytoplasm, and is markedly conservative and stable. MicroRNA (miR)-378a-3p and human (hsa)_circ_0006215 were detected using the reverse transcription-quantitative polymerase chain reaction (RT-qPCR) in tissue and cells...
July 2018: Oncology Letters
Xue-Feng Li, Ju-Kun Song, Jun-Wei Cai, Yu-Qin Zeng, Min Li, Jie Zhu, Yu-Ming Niu
Previous epidemiologic studies have revealed a possible association between microRNA-608 rs4919510 G>C polymorphism and digestive system cancers (DSCs) risk, but the results were not consistent. We therefore performed an updated meta-analysis to explore the association between microRNA-608 rs4919510 G>C polymorphism and DSCs risk. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were calculated to assess the relationship between the microRNA-608 rs4919510 G>C polymorphism and DSCs risk. Heterogeneity, cumulative analyses, sensitivity analyses, and publication bias were also conducted to examine the statistical power...
2018: Frontiers in Physiology
Gaoping Zhao, Tao Jiang, Yanzhuo Liu, Guoli Huai, Chunbin Lan, Guiquan Li, Guiqing Jia, Kang Wang, Maozhu Yang
BACKGROUND: Novel non-invasive biomarkers for gastric cancer (GC) are needed, because the present diagnostic methods for GC are either invasive or insensitive and non-specific in clinic. The presence of stable circulating microRNAs (miRNAs) in plasma suggested a promising role as GC biomarkers. METHODS: Based on the quantitative droplet digital PCR (ddPCR), four miRNAs (miR-21, miR-93, miR-106a and miR-106b) related to the presence of GC were identified in plasma from a training cohort of 147 participants and a validation cohort of 28 participants...
June 22, 2018: BMC Cancer
Chia-Wei Lin, Ming-Shiou Jan, Jung-Hua Steven Kuo
Intercellular communication is known to be involved in various stages of tumor development and metastasis through the secretion of extracellular vesicles (EVs) containing messengers such as microRNAs (miRNAs). Therefore, this study explored miRNA profiles in cancer cell-derived EVs after non-viral gene delivery in order to better understand the molecular information of intercellular communication in cancer cells after gene delivery. Two commonly used non-viral vectors (Lipofectamine 2000 and jet polyethylenimine) were used for the delivery of gene fluorescent protein plasmid in HeLa cancer cells...
June 22, 2018: Journal of Drug Targeting
Xinbo Zhang, Nathan L Price, Carlos Fernández-Hernando
Cardiovascular disease (CVD), the leading cause of death and morbidity in the Western world, begins with lipid accumulation in the arterial wall, which is the initial step in atherogenesis. Alterations in lipid metabolism result in increased risk of cardiometabolic disorders, and treatment of lipid disorders remains the most common strategy aimed at reducing the incidence of CVD. Work done over the past decade has identified numerous classes of non-coding RNA molecules including microRNAs (miRNAs) and long-non-coding RNAs (lncRNAs) as critical regulators of gene expression involved in lipid metabolism and CVD, mostly acting at post-transcriptional level...
June 18, 2018: Vascular Pharmacology
Ling Qian, Shulin Yu, Zhen Chen, Zhiqiang Meng, Shenglin Huang, Peng Wang
Circular RNA (circRNA), a recently discovered subclass of non-coding RNAs (ncRNAs), forms a covalently closed loop with neither a 5' cap structure nor a 3' polyadenylated tail. Generated from precursor mRNA (pre-mRNA) through "backsplicing" (a type of alternative RNA splicing), the majority of circRNAs are located in the cytoplasm and are widespread among living organisms. They are stable and conserved and exhibit spatiotemporal-specific expression. circRNAs are known to be involved in the development and progression of multiple diseases, including cancer, by acting as microRNA (miRNA) sponges and by regulating processes such as transcription and translation...
June 18, 2018: Biochimica et Biophysica Acta
Yuan Yuan, Wei Liu, Yangmei Zhang, Youwei Zhang, Sanyuan Sun
Recently, circular RNA (circRNA) is identified as a novel class of noncoding RNA with important roles in human diseases, such as cancer. However, how circRNA participates in colorectal cancer (CRC) remains unclear. In this study, we aimed to illustrate the role of circ_0026344 in CRC progression. We showed that circ_0026344 expression was downregulated in CRC tissues compared to adjacent normal tissues. Moreover, the level of circ_0026344 was inversely correlated with CRC advance and lymphoid node metastasis...
June 18, 2018: Biochemical and Biophysical Research Communications
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