keyword
https://read.qxmd.com/read/31771337/inpatient-antibiotic-stewardship-interventions-in-the-adult-oncology-and-hematopoietic-stem-cell-transplant-population-a-review-of-the-literature
#21
REVIEW
Kelly E Pillinger, Jeannette Bouchard, Sarah T Withers, Krutika Mediwala, Edoabasi U McGee, Geneen M Gibson, Christopher M Bland, P Brandon Bookstaver
Objective: To review the use of antibiotic stewardship interventions in the adult oncology and hematopoietic cell transplantation (HCT) populations. Data Sources: A literature search of PubMed was performed from inception to October 31, 2019. The general search terms used were oncology, cancer, hematologic malignancy, antimicrobial stewardship, antibiotic stewardship, febrile neutropenia, neutropenic fever, de-escalation, discontinuation, prophylaxis, practice guidelines, clinical pathway, rapid diagnostics, Filmarray, Verigene, MALDI-TOF, antibiotic allergy , and antimicrobial resistance ...
June 2020: Annals of Pharmacotherapy
https://read.qxmd.com/read/31751768/rapid-molecular-tests-for-detection-of-antimicrobial-resistance-determinants-in-gram-negative-organisms-from-positive-blood-cultures-a-systematic-review-and-meta-analysis
#22
JOURNAL ARTICLE
G De Angelis, A Grossi, G Menchinelli, S Boccia, M Sanguinetti, B Posteraro
BACKGROUND: Timely detection of antimicrobial (cephalosporin/carbapenem) resistance (AMR) determinants is crucial to the clinical management of bloodstream infections caused by Gram-negative bacteria (GNB). OBJECTIVES: To review and meta-analyse the evidence for using commercially available molecular tests for the direct detection of AMR determinants in GNB-positive blood cultures (PBCs). DATA SOURCES: PubMed, Scopus and ISI Web of Knowledge...
March 2020: Clinical Microbiology and Infection
https://read.qxmd.com/read/31065712/prospective-investigation-of-an-automated-pcr-nucleic-acid-microarray-based-platform-for-enteric-pathogen-testing
#23
JOURNAL ARTICLE
Amy K Beckman, Patricia Ferrieri
BACKGROUND: The Verigene Enteric Pathogens Test (Luminex Corporation) is a polymerase chain reaction (PCR)/nucleic acid microarray-based assay targeting 8 bacterial and viral pathogens that cause diarrhea. OBJECTIVE: To compare traditional enteric culture methods with stool testing by Verigene EP (PCR/microarray). METHODS: Tests were performed using PCR/microarray between February and August 2016. All specimens also underwent culture for Salmonella and Shigella; specimens that tested positive for bacterial pathogen(s) had confirmatory cultures...
May 8, 2019: Laboratory Medicine
https://read.qxmd.com/read/30690542/susceptibility-provision-enhances-effective-de-escalation-speed-utilizing-rapid-phenotypic-susceptibility-testing-in-gram-negative-bloodstream-infections-and-its-potential-clinical-impact
#24
JOURNAL ARTICLE
Jack G Schneider, James B Wood, Bryan H Schmitt, Christopher L Emery, Thomas E Davis, Nathan W Smith, Sarah Blevins, Jon Hiles, Armisha Desai, Justin Wrin, Brittany Bocian, John J Manaloor
Objectives: We evaluated the performance and time to result for pathogen identification (ID) and antimicrobial susceptibility testing (AST) of the Accelerate Pheno™ system (AXDX) compared with standard of care (SOC) methods. We also assessed the hypothetical improvement in antibiotic utilization if AXDX had been implemented. Methods: Clinical samples from patients with monomicrobial Gram-negative bacteraemia were tested and compared between AXDX and the SOC methods of the VERIGENE® and Bruker MALDI Biotyper® systems for ID and the VITEK® 2 system for AST...
January 1, 2019: Journal of Antimicrobial Chemotherapy
https://read.qxmd.com/read/30630335/-is-bc-gn-applicable-for-analysis-of-strains-and-urine-sputum-specimens
#25
JOURNAL ARTICLE
Tomoko Ohno, Hiroyuki Suematsu, Yuka Yamagishi, Narimi Miyazaki, Atsuko Yamada, Yuzuka Kawamoto, Daisuke Sakanashi, Isao Koita, Hiroshige Mikamo
As the reagent that can simultaneously detect bacterial nucleic acid/drug-resistant genes from the culture-positive liquid of blood cultures, Verigene® system includes the Verigene® Gram-Positive Blood Culture test (BC-GP) and the Verigene® Gram Negative Blood Culture test (BC-GN). This study used BC-GN to identify the names of bacteria from stock strains, urine samples, and sputum specimens and detect drug-resistant genes. The stock strains included 28 clinical isolates, 9 urine samples in which the target bacterial strain grew to 106CFU/ml or more in culture, and 9 sputum specimens in which the target bacterial strain grew to 105CFU/ml or more in culture...
December 25, 2018: JARMAM: Journal of the Association for Rapid Method and Automation in Microbiology
https://read.qxmd.com/read/30568975/validation-of-an-antimicrobial-stewardship-driven-verigene-blood-culture-gram-negative-treatment-algorithm-to-improve-appropriateness-of-antibiotics
#26
JOURNAL ARTICLE
Kimberly C Claeys, K E Schlaffer, E L Heil, S Leekha, J K Johnson
Rapid diagnostic testing (RDT) allows for early adjustment of antibiotic therapy. This study examined the potential impact of a stewardship-driven antibiotic treatment algorithm, incorporating RDT into the management of Gram-negative bacteremia. The proposed algorithm would have resulted in 88.4% of cases receiving appropriate antibiotic therapy versus 78.1% by standard of care ( P = .014).
October 2018: Open Forum Infectious Diseases
https://read.qxmd.com/read/30559141/the-hypothetical-impact-of-accelerate-pheno-acc-on-time-to-effective-therapy-and-time-to-definitive-therapy-for-bloodstream-infections-due-to-drug-resistant-gram-negative-bacilli
#27
JOURNAL ARTICLE
Oryan Henig, Keith S Kaye, Suganya Chandramohan, Christopher C Cooper, Paul Lephart, Hossein Salimnia, Maureen Taylor, Jason M Pogue
BACKGROUND: Strategies are needed to improve time to optimal therapy in patients with bloodstream infections (BSI) due to resistant Gram-negative (GN) pathogens. Accelerate Pheno™ (ACC) can provide antimicrobial susceptibility results within 7 hours of a positive culture and may more rapidly optimize therapy. OBJECTIVES: The primary objective was to evaluate the hypothetical impact of ACC on time to effective therapy (TTET) and time to definitive therapy (TTDT) among patients with BSI due to resistant GN pathogens...
December 17, 2018: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/30477953/reliability-of-the-verigene-system-for-the-identification-for-gram-positive-bacteria-and-detection-of-antimicrobial-resistance-markers-from-children-with-bacteremia
#28
COMPARATIVE STUDY
Martina Beckman, Matthew C Washam, Barbara DeBurger, David B Haslam, Joshua D Courter, Heidi Andersen, Joshua K Schaffzin, Michael J Tchou, Andrea Ankrum, Joel Mortensen
BACKGROUND: Targeted antimicrobial therapy can reduce morbidity in patients with sepsis. Molecular methodologies used in the clinical laboratory can provide information about infectious agents faster than traditional culture methods. Using molecular information to make clinical decisions more quickly has been shown to improve patient outcomes, and reduce length of stay and healthcare cost in adults. Its effect on pediatric care is less well described. METHODS: Blood cultures growing Gram-positive cocci or Gram-positive bacilli on Gram stain were evaluated by molecular and traditional methodologies...
March 2019: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/30239599/rapid-molecular-diagnostics-to-inform-empiric-use-of-ceftazidime-avibactam-and-ceftolozane-tazobactam-against-pseudomonas-aeruginosa-primers-iv
#29
JOURNAL ARTICLE
Scott R Evans, Thuy Tien T Tran, Andrea M Hujer, Carol B Hill, Kristine M Hujer, Jose R Mediavilla, Claudia Manca, T Nicholas Domitrovic, Federico Perez, Michael Farmer, Kelsey M Pitzer, Brigid M Wilson, Barry N Kreiswirth, Robin Patel, Michael R Jacobs, Liang Chen, Vance G Fowler, Henry F Chambers, Robert A Bonomo
BACKGROUND: Overcoming β-lactam resistance in pathogens such as Pseudomonas aeruginosa is a major clinical challenge. Rapid molecular diagnostics (RMDs) have the potential to inform selection of empiric therapy in patients infected by P. aeruginosa. METHODS: In this study, we used a heterogeneous collection of 197 P. aeruginosa that included multidrug-resistant isolates to determine whether 2 representative RMDs (Acuitas Resistome test and VERIGENE gram-negative blood culture test) could identify susceptibility to 2 newer β-lactam/β-lactamase inhibitor (BL-BLI) combinations, ceftazidime/avibactam (CZA) and ceftolozane/tazobactam (TOL/TAZO)...
May 17, 2019: Clinical Infectious Diseases
https://read.qxmd.com/read/30099638/a-5-year-study-of-the-performance-of-the-verigene-gram-positive-blood-culture-panel-in-a-pediatric-hospital
#30
JOURNAL ARTICLE
Chairut Vareechon, Javier Mestas, Claudia M Polanco, Jennifer Dien Bard
High accuracy of direct from positive blood culture molecular panels is imperative, particularly for the detection of resistance determinants as it allows for antimicrobial optimization prior to conventional susceptibility testing. In this study, we provide extensive data since implementation of the Verigene Gram-positive blood culture panel (BC-GP) in 2013. Within 5 years, 1636 blood culture bottles positive for a Gram-positive organism were tested on the BC-GP panel. The BC-GP panel identified 1520 Gram-positive organisms in 1636 (92...
November 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://read.qxmd.com/read/29899000/clinical-evaluation-of-the-icubate-ic-gpc-assay-for-detection-of-gram-positive-bacteria-and-resistance-markers-from-positive-blood-cultures
#31
JOURNAL ARTICLE
Paul A Granato, Melissa M Unz, Raymond H Widen, Suzane Silbert, Stephen Young, Kathryn L Heflin, Matt S Conover, Blake W Buchan, Nathan A Ledeboer
The iC-GPC Assay (iCubate, Huntsville, AL) is a qualitative multiplex test for the detection of five of the most common Gram-positive bacteria ( Staphylococcus aureus , Staphylococcus epidermidis , Streptococcus pneumoniae , Enterococcus faecalis , and Enterococcus faecium ) responsible for bacterial bloodstream infections, performed directly from positive blood cultures. The assay also detects the presence of the mecA , vanA , and vanB resistance determinants. This study comparatively evaluated the performance of the iC-GPC Assay against the Verigene Gram-positive blood culture (BC-GP) assay (Luminex Corp...
September 2018: Journal of Clinical Microbiology
https://read.qxmd.com/read/29483115/an-antibiotic-stewardship-program-blueprint-for-optimizing-verigene-bc-gn-within-an-institution-a-tale-of-two-cities
#32
JOURNAL ARTICLE
Jason M Pogue, Emily L Heil, Paul Lephart, J Kristie Johnson, Ryan P Mynatt, Hossein Salimnia, Kimberly C Claeys
Rapid diagnostic tests (RDTs) have revolutionized the management of Gram-negative bacteremia by allowing antimicrobial stewardship teams the ability to escalate therapy and improve patient outcomes through timely organism identification and detection of certain resistance determinants. However, given the complex nature of Gram-negative resistance, stewardship teams are left without clear direction for how to respond when resistance determinants are absent, as the safety of de-escalation in this setting is unknown...
May 2018: Antimicrobial Agents and Chemotherapy
https://read.qxmd.com/read/29449043/the-verigene-dilemma-gram-negative-polymicrobial-bloodstream-infections-and-clinical-decision-making
#33
JOURNAL ARTICLE
Kimberly C Claeys, Emily L Heil, Jason M Pogue, Paul R Lephart, J Kristie Johnson
Verigene Blood-Culture Gram-Negative (GN) results in rapid identification of key GNs in bloodstream infections. Its use clinically is limited by low sensitivity in polymicrobial GN infections and concerns for inappropriate antibiotic modification. In a retrospective review of 1003 blood culture sets, the incidence of missed GNs was infrequent, <4%, with the potential to negatively impact the management of GN BSIs in <2% of cases.
June 2018: Diagnostic Microbiology and Infectious Disease
https://read.qxmd.com/read/29258920/optimization-of-antimicrobial-therapy-in-vancomycin-resistant-enterococcal-bacteraemia-using-a-rapid-detection-gram-positive-blood-culture-assay
#34
JOURNAL ARTICLE
R Nakagawa, R Jain, A B Bryan, J D Chan
Rapid molecular blood culture Gram-positive (BC-GP) assay can promptly identify vancomycin-resistant enterococcal (VRE) bloodstream infections (BSIs). We sought to evaluate patients with VRE BSI following the pre (N = 44) and post (N = 20) implementation of Verigene BC-GP assay. The average time to detection of VRE was 25.9 ± 4.1h (95% confidence interval (CI): 17.6-34.1; P < 0.001) earlier with Verigene BC-GP assay. Compared to patients in the pre-Verigene BC-GP period, the mean adjusted difference in time to administration of anti-VRE therapy was 18...
June 2018: Journal of Hospital Infection
https://read.qxmd.com/read/29208560/multiplex-pcr-system-for-the-rapid-diagnosis-of-respiratory-virus-infection-systematic-review-and-meta-analysis
#35
REVIEW
H-S Huang, C-L Tsai, J Chang, T-C Hsu, S Lin, C-C Lee
OBJECTIVES: To provide a summary of evidence for the diagnostic accuracies of three multiplex PCR systems (mPCRs)-BioFire FilmArray RP (FilmArray), Nanosphere Verigene RV+ test (Verigene RV+) and Hologic Gen-Probe Prodesse assays-on the detection of viral respiratory infections. METHODS: A comprehensive search up to 1 July 2017 was conducted on Medline and Embase for studies that utilized FilmArray, Verigene RV+ and Prodesse for diagnosis of viral respiratory infections...
October 2018: Clinical Microbiology and Infection
https://read.qxmd.com/read/29020181/a-healthcare-improvement-intervention-combining-nucleic-acid-microarray-testing-with-direct-physician-response-for-management-of-staphylococcus-aureus-bacteremia
#36
JOURNAL ARTICLE
Joshua C Eby, Morgan M Richey, James A Platts-Mills, Amy J Mathers, Wendy M Novicoff, Heather L Cox
Background: Nucleic acid microarray (NAM) testing for detection of Staphylococcus aureus bacteremia (SAB) and S. aureus resistance gene determinants can reduce time to targeted antibiotic administration. Evidence-based management of SAB includes bedside infectious diseases (ID) consultation. As a healthcare improvement initiative at our institution, with the goal of improving management and outcomes for subjects with SAB, we implemented NAM with a process for responding to positive NAM results by directly triggered, mandatory ID consultation...
January 6, 2018: Clinical Infectious Diseases
https://read.qxmd.com/read/28966443/clinical-uptake-of-antimicrobial-stewardship-recommendations-following-nanosphere-verigene-blood-culture-gram-negative-reporting
#37
JOURNAL ARTICLE
Aaron Belknap, Daniel S Grosser, Daniel A Hale, Benjamin J Lang, Peter Colley, Raul Benavides, Neelam Dhiman
We performed a retrospective chart review of patients to determine if the Verigene Gram-negative blood culture (BC-GN) results would lead to earlier deescalation of empiric therapy for inpatients with GN bacteremia with Citrobacter spp., Enterobacter spp., Klebsiella spp., and Escherichia coli to appropriate targeted coverage. A total of 899 records were reviewed from April 2014 to February 2016 from three institutions within the Baylor Scott & White Health network. The cases were reviewed for initial antibiotic coverage, timing of Verigene results, change in antibiotic coverage, and how these changes related to the timing of Verigene results...
October 2017: Proceedings of the Baylor University Medical Center
https://read.qxmd.com/read/28961942/sustained-impact-of-a-rapid-microarray-based-assay-with-antimicrobial-stewardship-interventions-on-optimizing-therapy-in-patients-with-gram-positive-bacteraemia
#38
JOURNAL ARTICLE
Edina Avdic, Ruibin Wang, David X Li, Pranita D Tamma, Stephanie E Shulder, Karen C Carroll, Sara E Cosgrove
Objectives: To compare antibiotic optimization and outcomes of patients before implementation of the Verigene Gram-Positive Blood Culture (Verigene BC-GP) nucleic acid microarray assay to after implementation with antimicrobial stewardship (AS) interventions and after discontinuation of AS interventions. Methods: A retrospective pre-post-post quasi-experimental study was conducted to compare the three periods. AS interventions consisted of real-time guidance to clinicians on antibiotic selection...
November 1, 2017: Journal of Antimicrobial Chemotherapy
https://read.qxmd.com/read/28885937/utility-of-the-nanosphere-verigene-in-the-identification-of-bacteremia-isolates-from-the-burn-intensive-care-unit
#39
JOURNAL ARTICLE
Aaron Farmer, Charlotte A Lanteri, Eric Steele, Katrin Mende, Jeremy Pamplin, Kevin S Akers
INTRODUCTION: The Nanosphere Verigene Blood Culture Nucleic Acid Tests allow pathogen and antimicrobial resistance marker identification within 2.5 hours of a positive blood culture. This study assessed the sensitivity of the Verigene among Burn Intensive Care Unit (BICU) isolates and acceleration to targeted antibiotic therapy. METHODS: Bacterial identifications from BICU patients with positive blood cultures over 8 months were compared using 2 different platforms, the Verigene Gram-positive and Gram-negative blood culture tests vs...
September 2017: Military Medicine
https://read.qxmd.com/read/28751156/performance-evaluation-of-the-verigene-%C3%A2-clostridium-difficile-nucleic-acid-test-an-automated-multiplex-molecular-testing-system-for-detection-of-c-difficile-toxin
#40
JOURNAL ARTICLE
Kosuke Kosai, Yuki Iwanaga, Norihiko Akamatsu, Yuya Okada, Norihito Kaku, Naoki Uno, Yoshitomo Morinaga, Hiroo Hasegawa, Taiga Miyazaki, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara
The Verigene® Clostridium difficile nucleic acid test (Verigene® CDF test) is an automatic and rapid detection system for the genes encoding tcdA, tcdB, binary toxin, and the single nucleotide deletion at base pair 117 in the tcdC based on microarray and PCR amplification. We compared the performance of the Verigene® CDF test to that of two enzyme immunoassays, C. DIFF QUIK CHEK COMPLETE and X/Pect Toxin A/B, using 118 specimens. We found overall concordance rates of 81.4% and 78.8% between C. DIFF QUIK CHEK COMPLETE and Verigene® CDF test, and X/Pect Toxin A/B and Verigene® CDF test...
October 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
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