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https://www.readbyqxmd.com/read/29208560/multiplex-pcr-system-for-the-rapid-diagnosis-of-respiratory-virus-infection-a-systematic-review-and-meta-analysis
#1
REVIEW
Huai-Shiuan Huang, Chi-Lin Tsai, Jeffrey Chang, Tzu-Chun Hsu, Samuel Lin, Chien-Chang Lee
OBJECTIVES: We aim to provide a summary of evidence for the diagnostic accuracies of three multiplex PCR systems (mPCRs): BioFire Filmarray RP (Filmarray), Nanosphere Verigene RV+ test (Verigene) and Hologic Gen-Probe Prodesse assays on the detection of viral respiratory infections. METHODS: A comprehensive search up to July 1st, 2017 was conducted on Medline and Embase for studies that utilized Filmarray, Verigene and Prodesse for diagnosis of viral respiratory infections...
December 2, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/29020181/a-healthcare-improvement-intervention-combining-nucleic-acid-microarray-testing-with-direct-physician-response-for-management-of-staphyloccocus-aureus-bacteremia
#2
Joshua C Eby, Morgan M Richey, James A Platts-Mills, Amy J Mathers, Wendy M Novicoff, Heather L Cox
Background: Nucleic acid microarray (NAM) testing for detection of Staphylococcus aureus bacteremia (SAB) and S. aureus resistance gene determinants can reduce time to targeted antibiotic administration. Evidence-based management of SAB includes bedside Infectious Diseases (ID) consultation. As a healthcare improvement initiative at our institution, with the goal of improving management and outcomes for subjects with SAB, we implemented NAM with a process for responding to positive NAM results by directly triggered, mandatory ID consultation...
August 17, 2017: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/28966443/clinical-uptake-of-antimicrobial-stewardship-recommendations-following-nanosphere-verigene-blood-culture-gram-negative-reporting
#3
Aaron Belknap, Daniel S Grosser, Daniel A Hale, Benjamin J Lang, Peter Colley, Raul Benavides, Neelam Dhiman
We performed a retrospective chart review of patients to determine if the Verigene Gram-negative blood culture (BC-GN) results would lead to earlier deescalation of empiric therapy for inpatients with GN bacteremia with Citrobacter spp., Enterobacter spp., Klebsiella spp., and Escherichia coli to appropriate targeted coverage. A total of 899 records were reviewed from April 2014 to February 2016 from three institutions within the Baylor Scott & White Health network. The cases were reviewed for initial antibiotic coverage, timing of Verigene results, change in antibiotic coverage, and how these changes related to the timing of Verigene results...
October 2017: Proceedings of the Baylor University Medical Center
https://www.readbyqxmd.com/read/28961942/sustained-impact-of-a-rapid-microarray-based-assay-with-antimicrobial-stewardship-interventions-on-optimizing-therapy-in-patients-with-gram-positive-bacteraemia
#4
Edina Avdic, Ruibin Wang, David X Li, Pranita D Tamma, Stephanie E Shulder, Karen C Carroll, Sara E Cosgrove
Objectives: To compare antibiotic optimization and outcomes of patients before implementation of the Verigene Gram-Positive Blood Culture (Verigene BC-GP) nucleic acid microarray assay to after implementation with antimicrobial stewardship (AS) interventions and after discontinuation of AS interventions. Methods: A retrospective pre-post-post quasi-experimental study was conducted to compare the three periods. AS interventions consisted of real-time guidance to clinicians on antibiotic selection...
November 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28885937/utility-of-the-nanosphere-verigene-in-the-identification-of-bacteremia-isolates-from-the-burn-intensive-care-unit
#5
Aaron Farmer, Charlotte A Lanteri, Eric Steele, Katrin Mende, Jeremy Pamplin, Kevin S Akers
INTRODUCTION: The Nanosphere Verigene Blood Culture Nucleic Acid Tests allow pathogen and antimicrobial resistance marker identification within 2.5 hours of a positive blood culture. This study assessed the sensitivity of the Verigene among Burn Intensive Care Unit (BICU) isolates and acceleration to targeted antibiotic therapy. METHODS: Bacterial identifications from BICU patients with positive blood cultures over 8 months were compared using 2 different platforms, the Verigene Gram-positive and Gram-negative blood culture tests vs...
September 2017: Military Medicine
https://www.readbyqxmd.com/read/28751156/performance-evaluation-of-the-verigene-%C3%A2-clostridium-difficile-nucleic-acid-test-an-automated-multiplex-molecular-testing-system-for-detection-of-c-%C3%A2-difficile-toxin
#6
Kosuke Kosai, Yuki Iwanaga, Norihiko Akamatsu, Yuya Okada, Norihito Kaku, Naoki Uno, Yoshitomo Morinaga, Hiroo Hasegawa, Taiga Miyazaki, Koichi Izumikawa, Hiroshi Mukae, Katsunori Yanagihara
The Verigene(®)Clostridium difficile nucleic acid test (Verigene(®) CDF test) is an automatic and rapid detection system for the genes encoding tcdA, tcdB, binary toxin, and the single nucleotide deletion at base pair 117 in the tcdC based on microarray and PCR amplification. We compared the performance of the Verigene(®) CDF test to that of two enzyme immunoassays, C. DIFF QUIK CHEK COMPLETE and X/Pect Toxin A/B, using 118 specimens. We found overall concordance rates of 81.4% and 78.8% between C. DIFF QUIK CHEK COMPLETE and Verigene(®) CDF test, and X/Pect Toxin A/B and Verigene(®) CDF test...
October 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
https://www.readbyqxmd.com/read/28742143/impact-of-rapid-identification-of-positive-blood-cultures-using-the-verigene-system-on-antibiotic-prescriptions-a-prospective-study-of-community-onset-bacteremia-in-a-tertiary-hospital-in-japan
#7
Kayoko Hayakawa, Kazuhisa Mezaki, Masao Kobayakawa, Kei Yamamoto, Yoshikazu Mutoh, Motoyuki Tsuboi, Takehiro Hasimoto, Maki Nagamatsu, Satoshi Kutsuna, Nozomi Takeshita, Yuichi Katanami, Masahiro Ishikane, Norio Ohmagari
BACKGROUND: Rapid identification of positive blood cultures is important for initiation of optimal treatment in septic patients. Effects of automated, microarray-based rapid identification systems on antibiotic prescription against community-onset bacteremia (COB) remain unclear. METHODS: We prospectively enrolled 177 patients with 185 COB episodes (occurring within 72 h of admission) over 17 months. Bacteremia episodes due to gram-positive bacteria (GP) and gram-negative bacteria (GN) in the same patient were counted separately...
2017: PloS One
https://www.readbyqxmd.com/read/28637912/parallel-evaluation-of-the-maldi-sepsityper-and-verigene-bc-gn-assays-for-rapid-identification-of-gram-negative-bacilli-from-positive-blood-cultures
#8
Miguel A Arroyo, Gerald A Denys
Rapid identification of microorganisms from positive blood cultures has improved clinical management and antimicrobial stewardship. The advent of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has reduced the time to identification of cultured isolates and is now often the definitive method used in the clinical microbiology laboratory. The commercial in vitro diagnostic MALDI Sepsityper (Sepsityper) kit has the potential for standardization and clinical routine use for the rapid identification of a broad range of bacteria from positive blood cultures...
September 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28534213/impact-of-antimicrobial-stewardship-and-rapid-microarray-testing-on-patients-with-gram-negative-bacteremia
#9
K R Rivard, V Athans, S W Lam, S M Gordon, G W Procop, S S Richter, E Neuner
A rapid microarray assay, Nanosphere Verigene® Gram-negative blood culture test (BC-GN), detects four Gram-negative species, four Gram-negative genera, and six resistance genes directly from positive blood culture samples, shortening the time from Gram stain to pathogen and resistance-gene identification. The purpose of this study was to determine the impact of the BC-GN paired with an antimicrobial stewardship intervention on antimicrobial and clinical outcomes. Patients with Gram-negative bacteremia were compared before (n = 456) and after (n = 421) BC-GN implementation...
October 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28405178/utilizing-bd-max%C3%A2-enteric-bacterial-panel-to-detect-stool-pathogens-from-rectal-swabs
#10
Barbara DeBurger, Sarah Hanna, Eleanor A Powell, Cindi Ventrola, Joel E Mortensen
BACKGROUND: The BD MAX™ Enteric Bacterial Panel (BDM-EBP) is designed and FDA-cleared to detect Salmonella, Shigella, Campylobacter, and Shiga toxin genes stx1/2 from stool samples. However, rectal swabs, which are not FDA-cleared for clinical testing with the BDM-EBP, are common specimens received from pediatric patients for enteric pathogen testing. The purpose of this study was to evaluate the ability of the BDM-EBP to detect stool pathogens from rectal swabs. METHODS: Routine cultures, Shiga toxin testing, and molecular testing with BDM-EBP were performed on 272 sequential rectal swabs collected from August 2015 to December 2015...
2017: BMC Clinical Pathology
https://www.readbyqxmd.com/read/28316631/potential-impact-of-rapid-blood-culture-testing-for-gram-positive-bacteremia-in-japan-with-the-verigene-gram-positive-blood-culture-test
#11
Ken Kikuchi, Mari Matsuda, Shigekazu Iguchi, Tomonori Mizutani, Keiichi Hiramatsu, Michiru Tega-Ishii, Kaori Sansaka, Kenta Negishi, Kimie Shimada, Jun Umemura, Shigeyuki Notake, Hideji Yanagisawa, Hiroshi Takahashi, Reiko Yabusaki, Hideki Araoka, Akiko Yoneyama
Background. Early detection of Gram-positive bacteremia and timely appropriate antimicrobial therapy are required for decreasing patient mortality. The purpose of our study was to evaluate the performance of the Verigene Gram-positive blood culture assay (BC-GP) in two special healthcare settings and determine the potential impact of rapid blood culture testing for Gram-positive bacteremia within the Japanese healthcare delivery system. Furthermore, the study included simulated blood cultures, which included a library of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) isolates reflecting different geographical regions in Japan...
2017: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/28222641/genotyping-guided-approach-versus-the-conventional-approach-in-selection-of-oral-p2y12-receptor-blockers-in-chinese-patients-suffering-from-acute-coronary-syndrome
#12
RANDOMIZED CONTROLLED TRIAL
Chor Cheung Tam, Janette Kwok, Anthony Wong, Arthur Yung, Catherine Shea, Shun Ling Kong, Wing Hong Tang, David Siu, Raymond Chan, Stephen Lee
Objective The CYP2C19 loss-of-function (LoF) allele is present in half of the East Asian population and is associated with high on-treatment platelet reactivity (HTPR). This study aimed to investigate whether a rapid genotyping-guided approach is feasible and efficacious for selecting P2Y12 receptor blockers in Chinese patients suffering from acute coronary syndrome (ACS). Methods This was a single-centre, prospective, randomized, open-label study. A total of 132 patients with ACS were randomized to the rapid genotyping-guided treatment group (GG, N = 65) or the standard treatment group (SG, N = 67)...
February 2017: Journal of International Medical Research
https://www.readbyqxmd.com/read/27818808/persistent-bacteremia-from-pseudomonas-aeruginosa-with-in-vitro-resistance-to-the-novel-antibiotics-ceftolozane-tazobactam-and-ceftazidime-avibactam
#13
Louie Mar Gangcuangco, Patricia Clark, Cynthia Stewart, Goran Miljkovic, Zane K Saul
Ceftazidime-avibactam and ceftolozane-tazobactam are new antimicrobials with activity against multidrug-resistant Pseudomonas aeruginosa. We present the first case of persistent P. aeruginosa bacteremia with in vitro resistance to these novel antimicrobials. A 68-year-old man with newly diagnosed follicular lymphoma was admitted to the medical intensive care unit for sepsis and right lower extremity cellulitis. The patient was placed empirically on vancomycin and piperacillin-tazobactam. Blood cultures from Day 1 of hospitalization grew P...
2016: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/27767002/experience-with-rapid-microarray-based-diagnostic-technology-and-antimicrobial-stewardship-for-patients-with-gram-positive-bacteremia
#14
Elizabeth A Neuner, Andrea M Pallotta, Simon W Lam, David Stowe, Steven M Gordon, Gary W Procop, Sandra S Richter
OBJECTIVE To describe the impact of rapid diagnostic microarray technology and antimicrobial stewardship for patients with Gram-positive blood cultures. DESIGN Retrospective pre-intervention/post-intervention study. SETTING A 1,200-bed academic medical center. PATIENTS Inpatients with blood cultures positive for Staphylococcus aureus, Enterococcus faecalis, E. faecium, Streptococcus pneumoniae, S. pyogenes, S. agalactiae, S. anginosus, Streptococcus spp., and Listeria monocytogenes during the 6 months before and after implementation of Verigene Gram-positive blood culture microarray (BC-GP) with an antimicrobial stewardship intervention...
November 2016: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/27720206/performance-of-the-verigene%C3%A2-enteric-pathogens-test-biofire-filmarray%C3%A2-gastrointestinal-panel-and-luminex-xtag%C3%A2-gastrointestinal-pathogen-panel-for-detection-of-common-enteric-pathogens
#15
COMPARATIVE STUDY
Richard S P Huang, Coreen L Johnson, Lauryn Pritchard, Richard Hepler, Trang T Ton, James J Dunn
BACKGROUND: Multiplex syndromic panels have the capability of identifying causes of diarrheal illness. This study evaluated the performance characteristics of three multiplex molecular assays for the detection of common stool pathogens. METHODS: A total of 152 stool specimens were tested using three platforms: Verigene Enteric Pathogens Test (Verigene), Biofire FilmArray Gastrointestinal Panel (Biofire) and Luminex xTAG® Gastrointestinal Pathogen Panel (Luminex)...
December 2016: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27624327/comparison-of-rapid-hybridization-based-pathogen-identification-and-resistance-evaluation-in-sepsis-using-the-verigene%C3%A2-device-paired-with-good-old-culture
#16
Michael Berktold, Wolfgang Mutschlechner, Dorothea Orth-Höller
Rapid microbial diagnostics is important for septicemic patients. The current gold standard is blood culture with consecutive pathogen identification and antimicrobial susceptibility testing. However, these culture-based methods need at least 48 h.The aim of this study was to compare Verigene(®) (Nanosphere, Northbrook, IL, USA), a rapid hybridization-based method, with conventional culture-based methods for detection of pathogens and resistance markers from positive blood cultures of septic patients.In 85 of 100 tested blood culture samples (85 %), pathogen identification as well as resistance profile were identical in Verigene and conventional culture...
June 2017: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/27599796/implementation-of-rapid-diagnostics-with-antimicrobial-stewardship
#17
REVIEW
Emi Minejima, Annie Wong-Beringer
Antimicrobial stewardship (ASP) is an intervention-based program to improve patient outcomes to infection while limiting spread of resistance and unintended consequences. Many rapid diagnostic tools are now FDA cleared for clinical use, with three evaluated across multiple settings: Matrix Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry, Verigene, and FilmArray. Areas covered: This review will focus on studies published that evaluated ASP intervention with rapid diagnostic implementation on outcomes of infection...
November 2016: Expert Review of Anti-infective Therapy
https://www.readbyqxmd.com/read/27098961/clinical-impact-of-laboratory-implementation-of-verigene-bc-gn-microarray-based-assay-for-detection-of-gram-negative-bacteria-in-positive-blood-cultures
#18
Tamar Walker, Sandrea Dumadag, Christine Jiyoun Lee, Seung Heon Lee, Jeffrey M Bender, Jennifer Cupo Abbott, Rosemary C She
Gram-negative bacteremia is highly fatal, and hospitalizations due to sepsis have been increasing worldwide. Molecular tests that supplement Gram stain results from positive blood cultures provide specific organism information to potentially guide therapy, but more clinical data on their real-world impact are still needed. We retrospectively reviewed cases of Gram-negative bacteremia in hospitalized patients over a 6-month period before (n = 98) and over a 6-month period after (n = 97) the implementation of a microarray-based early identification and resistance marker detection system (Verigene BC-GN; Nanosphere) while antimicrobial stewardship practices remained constant...
July 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27026755/the-frequency-of-some-thrombophilic-mutations-in-eastern-turkey
#19
Nurinnisa Ozturk, Ebubekir Bakan, Mehmet Ali Gul, Nuri Bakan, Engin Sebin, Ahmet Kiziltunc
OBJECTIVE: Factor V / Factor II / Methylenetetrahydrofolate reductase, gene polymorphisms are closely associated with thrombophilia. Regional frequencies of these mutations may show a characteristic state. The aim of our study was to evaluate the frequency of commonly seen Factor V / Factor II / Methylenetetrahydrofolate reductase gene polymorphisms in Eastern Turkey. MATERIALS AND METHODS: In 433 patients sent to the laboratory with the suspicion of thrombophilia, using whole blood samples, an automated Nucleic Acid Test was used for mutation determinations in Verigene System...
February 2016: Eurasian Journal of Medicine
https://www.readbyqxmd.com/read/26904669/evaluation-of-verigene-blood-culture-test-systems-for-rapid-identification-of-positive-blood-cultures
#20
Jae-Seok Kim, Go-Eun Kang, Han-Sung Kim, Hyun Soo Kim, Wonkeun Song, Kyu Man Lee
The performance of molecular tests using the Verigene Gram-Positive and Gram-Negative Blood Culture nucleic acid tests (BC-GP and BC-GN, resp.; Naosphere, Northbrook, IL, USA) was evaluated for the identification of microorganisms detected from blood cultures. Ninety-nine blood cultures containing Gram-positive bacteria and 150 containing Gram-negative bacteria were analyzed using the BC-GP and BC-GN assays, respectively. Blood cultures were performed using the Bactec blood culture system (BD Diagnostic Systems, Franklin Lakes, NJ, USA) and conventional identification and antibiotic-susceptibility tests were performed using a MicroScan system (Siemens, West Sacramento, CA, USA)...
2016: BioMed Research International
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