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https://www.readbyqxmd.com/read/29038332/gata-transcription-factors-in-testicular-adrenal-rest-tumours
#1
Manon Engels, Paul N Span, Rod T Mitchell, Joop J T M Heuvel, Monica A Marijnissen-van Zanten, Antonius Eduard van Herwaarden, Christina A Hulsbergen-van de Kaa, Egbert Oosterwijk, Nike M Stikkelbroeck, Lee B Smith, Fred C G J Sweep, Hedi L Claahsen-van der Grinten
Testicular adrenal rest tumours (TARTs) are benign adrenal-like testicular tumours that frequently occur in male patients with congenital adrenal hyperplasia. Recently, GATA transcription factors have been linked to the development of TARTs in mice. The aim of our study was to determine GATA expression in human TARTs and other steroidogenic tissues. We determined GATA expression in TARTs (n=16), Leydig cell tumours (LCTs; n=7), adrenal (foetal (n=6) + adult (n=10)), and testis (foetal (n=13) + adult (n=8))...
October 16, 2017: Endocrine Connections
https://www.readbyqxmd.com/read/29035371/lncrna-mir100hg-derived-mir-100-and-mir-125b-mediate-cetuximab-resistance-via-wnt-%C3%AE-catenin-signaling
#2
Yuanyuan Lu, Xiaodi Zhao, Qi Liu, Cunxi Li, Ramona Graves-Deal, Zheng Cao, Bhuminder Singh, Jeffrey L Franklin, Jing Wang, Huaying Hu, Tianying Wei, Mingli Yang, Timothy J Yeatman, Ethan Lee, Kenyi Saito-Diaz, Scott Hinger, James G Patton, Christine H Chung, Stephan Emmrich, Jan-Henning Klusmann, Daiming Fan, Robert J Coffey
De novo and acquired resistance, which are largely attributed to genetic alterations, are barriers to effective anti-epidermal-growth-factor-receptor (EGFR) therapy. To generate cetuximab-resistant cells, we exposed cetuximab-sensitive colorectal cancer cells to cetuximab in three-dimensional culture. Using whole-exome sequencing and transcriptional profiling, we found that the long non-coding RNA MIR100HG and two embedded microRNAs, miR-100 and miR-125b, were overexpressed in the absence of known genetic events linked to cetuximab resistance...
October 16, 2017: Nature Medicine
https://www.readbyqxmd.com/read/28977863/clinical-roles-of-the-aberrantly-expressed-lncrnas-in-lung-squamous-cell-carcinoma-a-study-based-on-rna-sequencing-and-microarray-data-mining
#3
Wen-Jie Chen, Rui-Xue Tang, Rong-Quan He, Dong-Yao Li, Liang Liang, Jiang-Hui Zeng, Xiao-Hua Hu, Jie Ma, Shi-Kang Li, Gang Chen
Lung squamous cell carcinoma (LUSC) accounts for a significant proportion of lung cancer and there have been few therapeutic alternatives for recurrent LUSC due to the lack of specific driver molecules. To investigate the prospective role of lncRNAs in the tumorigenesis and progression of LUSC, the aberrantly expressed lncRNAs were calculated based on The Cancer Genome Atlas RNA-seq data. Of 7589 lncRNAs with 504 LUSC cases, 884 lncRNAs were identified as being aberrantly expressed (|log2 fold change| >2 and adjusted P<0...
September 22, 2017: Oncotarget
https://www.readbyqxmd.com/read/28975155/genome-wide-scan-in-hispanics-highlights-candidate-loci-for-brain-white-matter-hyperintensities
#4
Ashley Beecham, Chuanhui Dong, Clinton B Wright, Nicole Dueker, Adam M Brickman, Liyong Wang, Charles DeCarli, Susan H Blanton, Tatjana Rundek, Richard Mayeux, Ralph L Sacco
OBJECTIVE: To investigate genetic variants influencing white matter hyperintensities (WMHs) in the understudied Hispanic population. METHODS: Using 6.8 million single nucleotide polymorphisms (SNPs), we conducted a genome-wide association study (GWAS) to identify SNPs associated with WMH volume (WMHV) in 922 Hispanics who underwent brain MRI as a cross-section of 2 community-based cohorts in the Northern Manhattan Study and the Washington Heights-Inwood Columbia Aging Project...
October 2017: Neurology. Genetics
https://www.readbyqxmd.com/read/28952437/a-tissue-specific-gata6-driven-transcriptional-program-instructs-remodeling-of-the-mature-arterial-tree
#5
Marta Losa, Victor Latorre, Munazah Andrabi, Franck Ladam, Charles Sagerström, Ana Novoa, Peyman Zarrineh, Laure Bridoux, Neil A Hanley, Moises Mallo, Nicoletta Bobola
Connection of the heart to the systemic circulation is a critical developmental event that requires selective preservation of embryonic vessels (aortic arches). However, why some aortic arches regress while others are incorporated into the mature aortic tree remains unclear. By microdissection and deep sequencing in mouse, we find that neural crest (NC) only differentiates into vascular smooth muscle cells (SMCs) around those aortic arches destined for survival and reorganization, and identify the transcription factor Gata6 as a crucial regulator of this process...
September 27, 2017: ELife
https://www.readbyqxmd.com/read/28943339/%C3%AE-catenin-coordinates-with-jup-and-the-tcf1-gata6-axis-to-regulate-human-embryonic-stem-cell-fate
#6
Hongwei Sun, Xiaohu Wang, Kuisheng Liu, Mengmeng Guo, Yan Zhang, Qi-Long Ying, Shoudong Ye
β-catenin-mediated signaling has been extensively studied in regard to its role in the regulation of human embryonic stem cells (hESCs). However, the results are controversial and the mechanism by which β-catenin regulates the hESC fate remains unclear. Here, we report that β-catenin and γ-catenin are functionally redundant in mediating hESC adhesion and are required for embryoid body formation, but both genes are dispensable for hESC maintenance, as the undifferentiated state of β-catenin and γ-catenin double deficient hESCs can be maintained...
September 21, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28943029/generation-of-mouse-and-human-organoid-forming-intestinal-progenitor-cells-by-direct-lineage-reprogramming
#7
Shizuka Miura, Atsushi Suzuki
Intestinal organoids hold great promise as a valuable tool for studying and treating intestinal diseases. The currently available sources of human intestinal organoids, tissue fragments or pluripotent stem cells, involve invasive procedures or complex differentiation protocols, respectively. Here, we show that a set of four transcription factors, Hnf4α, Foxa3, Gata6, and Cdx2, can directly reprogram mouse fibroblasts to acquire the identity of fetal intestine-derived progenitor cells (FIPCs). These induced FIPCs (iFIPCs) form spherical organoids that develop into adult-type budding organoids containing cells with intestinal stem cell properties...
October 5, 2017: Cell Stem Cell
https://www.readbyqxmd.com/read/28938416/pancreatic-histopathology-of-human-monogenic-diabetes-due-to-causal-variants-in-kcnj11-hnf1a-gata6-and-lmna
#8
May Sanyoura, Laura Jacobsen, David Carmody, Daniela Del Gaudio, Gorka Alkorta-Aranburu, Kelly Arndt, Ying Ying Hu, Frances Kobiernicki, Irina Kusmartseva, Mark A Atkinson, Louis H Philipson, Desmond Schatz, Martha Campbell-Thompson, Siri Atma W Greeley
Context: Monogenic diabetes is thought to account for 2% of all diabetes cases but most patients are misdiagnosed as type 1 or type 2 diabetes. To date, little is known about the histopathological features of pancreases from patients with monogenic diabetes. Objective: Retrospective study of the JDRF Network for Pancreatic Organ donors with Diabetes biorepository was used to identify possible cases with monogenic diabetes and to compare effects of genetic variants on pancreas histology...
August 16, 2017: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28935706/suppression-of-erk-signalling-abolishes-primitive-endoderm-formation-but-does-not-promote-pluripotency-in-rabbit-embryo
#9
Anna Piliszek, Zofia E Madeja, Berenika Plusa
Formation of epiblast (EPI) - the founder line of all embryonic lineages - and extra-embryonic supportive tissues is one of the key events in mammalian development. The prevailing model of early mammalian development is based almost exclusively on the mouse. Here we provide a comprehensive, stage-by-stage analysis of the EPI and extraembryonic primitive endoderm (PrE) formation during pre-implantation development of the rabbit. Whilst we observed that rabbit embryos have several features in common with mouse embryos, including a stage-related initiation of lineage specification, our results demonstrate the existence of some key differences in lineage specification among mammals...
September 21, 2017: Development
https://www.readbyqxmd.com/read/28877476/mtorc2-signaling-selectively-regulates-the-generation-and-function-of-tissue-resident-peritoneal-macrophages
#10
Min-Hee Oh, Samuel L Collins, Im-Hong Sun, Ada J Tam, Chirag H Patel, Matthew L Arwood, Yee Chan-Li, Jonathan D Powell, Maureen R Horton
Tissue-resident macrophages play critical roles in sentinel and homeostatic functions as well as in promoting inflammation and immunity. It has become clear that the generation of these cells is highly dependent upon tissue-specific cues derived from the microenvironment that, in turn, regulate unique differentiation programs. Recently, a role for GATA6 has emerged in the differentiation programming of resident peritoneal macrophages. We identify a critical role for mTOR in integrating cues from the tissue microenvironment in regulating differentiation and metabolic reprogramming...
September 5, 2017: Cell Reports
https://www.readbyqxmd.com/read/28870993/klf5-maintains-the-balance-of-primitive-endoderm-to-epiblast-specification-during-mouse-embryonic-development-by-suppression-of-fgf4
#11
Takuya Azami, Tsuyoshi Waku, Ken Matsumoto, Hyojung Jeon, Masafumi Muratani, Akihiro Kawashima, Jun Yanagisawa, Ichiro Manabe, Ryozo Nagai, Tilo Kunath, Tomonori Nakamura, Kazuki Kurimoto, Mitinori Saitou, Satoru Takahashi, Masatsugu Ema
The inner cell mass of the mouse blastocyst gives rise to the pluripotent epiblast (EPI), which forms the embryo proper, and the primitive endoderm (PrE), which forms extra-embryonic yolk sac tissues. All inner cells co-express lineage markers such as Nanog and Gata6 at embryonic day (E) 3.25, and the EPI and PrE precursor cells eventually segregate to exclusively express Nanog and Gata6, respectively. Fibroblast growth factor (FGF)/extracellular signal-regulated kinase (ERK) signalling is involved in segregation of the EPI and PrE lineages; however, the mechanism involved in Fgf4-regulation is poorly understood...
September 4, 2017: Development
https://www.readbyqxmd.com/read/28859285/bovine-lineage-specification-revealed-by-single-cell-gene-expression-analysis-from-zygote-to-blastocyst
#12
Qingqing Wei, Liang Zhong, Shaopeng Zhang, Haiyuan Mu, Jinzhu Xiang, Liang Yue, Yunping Dai, Jianyong Han
Preimplantation embryos undergo zygotic genome activation and lineage specification resulting in three distinct cell types in the late blastocyst. The molecular mechanisms underlying this progress are largely unknown in bovines. Here, we sought to analyze an extensive set of regulators at the single-cell level to define the events involved in the development of the bovine blastocyst. Using a quantitative microfluidics approach in single cells, we analyzed mRNA levels of 96 genes known to function in early embryonic development and maintenance of stem cell pluripotency in parallel in 384 individual cells from bovine preimplantation embryos...
July 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28841465/design-of-a-nanocomposite-substrate-inducing-adult-stem-cell-assembly-and-progression-toward-an-epiblast-like-or-primitive-endoderm-like-phenotype-via-mechanotransduction
#13
Francesco Morena, Ilaria Armentano, Pia Montanucci, Chiara Argentati, Elena Fortunati, Simona Montesano, Ilaria Bicchi, Teresa Pescara, Ilaria Pennoni, Samantha Mattioli, Luigi Torre, Loredana Latterini, Carla Emiliani, Giuseppe Basta, Riccardo Calafiore, Josè Maria Kenny, Sabata Martino
This work shows that the active interaction between human umbilical cord matrix stem cells and Poly (l-lactide)acid (PLLA) and PLLA/Multi Walled Carbon Nanotubes (MWCNTs) nanocomposite films results in the stem cell assembly as a spheroid conformation and affects the stem cell fate transition. We demonstrated that spheroids directly respond to a tunable surface and the bulk properties (electric, dielectric and thermal) of plain and nanocomposite PLLA films by triggering a mechanotransduction axis. This stepwise process starts from tethering of the cells' focal adhesion proteins to the surface, together with the adherens junctions between cells...
August 16, 2017: Biomaterials
https://www.readbyqxmd.com/read/28836411/evidence-of-oocyte-polarity-in-bovine-implications-for-intracytoplasmic-sperm-injection-and-somatic-cell-nuclear-transfer
#14
Seyed Morteza Hosseini, Fariba Moulavi, Nima TanhaieVash, Naser Shams-Esfandabadi, Mohammad Hossein Nasr-Esfahani, Abolfazl Shirazi
OBJECTIVES: We recently demonstrated spatial regionalization of maternal transcripts and proteins within unfertilized ovine oocyte. Here, we investigated the likelihood of oocyte polarity for the first time in bovine. MATERIALS AND METHODS: In this experimental study, in vitro matured bovine oocytes were used for manual bisection [into oocyte halve that were near-to (HNS) and far-from (FS) spindle] or trisection [into MII-spindle (S), the spindle-side half (NS), and the distal half unassociated with the spindle (FS)]...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28814615/single-cell-gene-expression-of-the-bovine-blastocyst
#15
Verónica M Negrón-Pérez, Yanping Zhang, Peter J Hansen
The first two differentiation events in the embryo result in three cell types - epiblast, trophectoderm (TE) and hypoblast. The purpose here was to identify molecular markers for each cell type in the bovine and evaluate the differences in gene expression among individual cells of each lineage. The cDNA from 67 individual cells of dissociated blastocysts was used to determine transcript abundance for 93 genes implicated as cell lineage markers in other species or potentially involved in developmental processes...
November 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28782694/co-regulation-of-micrornas-and-transcription-factors-in-cardiomyocyte-specific-differentiation-of-murine-embryonic-stem-cells-an-aspect-from-transcriptome-analysis
#16
Lin Gan, Bernd Denecke
The differentiation process of embryonic stem cells is a comprehensive process regulated by a variety of factors in response to stimulus. Studies of this process can be focused on cell biology as well as on molecular biology level. In this paper we identified the co-regulation of molecular regulators and their interactions during cardiomyocyte specific differentiation of mouse embryonic stem cells based on parallel genome wide transcriptome analyses of mRNA and microRNA. Differentially expressed mRNAs and microRNAs were identified according to their expression profiles...
August 4, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28737214/decline-in-cellular-function-of-aged-mouse-c-kit-cardiac-progenitor-cells
#17
Alessandra Castaldi, Ramsinh Mansinh Dodia, Amabel M Orogo, Cristina M Zambrano, Rita H Najor, Åsa B Gustafsson, Joan Heller Brown, Nicole H Purcell
KEY POINTS: While autologous stem cell-based therapies are currently being tested on elderly patients, there are limited data on the function of aged stem cells and in particular c-kit(+) cardiac progenitor cells (CPCs). We isolated c-kit(+) cells from young (3 months) and aged (24 months) C57BL/6 mice to compare their biological properties. Aged CPCs have increased senescence, decreased stemness and reduced capacity to proliferate or to differentiate following dexamethasone (Dex) treatment in vitro, as evidenced by lack of cardiac lineage gene upregulation...
October 1, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28710293/probing-gata-factor-function-in-mouse-leydig-cells-via-testicular-injection-of-adenoviral-vectors
#18
Gervette M Penny, Rebecca B Cochran, Marjut Pihlajoki, Antti Kyrönlahti, Anja Schrade, Merja Häkkinen, Jorma Toppari, Markku Heikinheimo, David B Wilson
Testicular Leydig cells produce androgens essential for proper male reproductive development and fertility. Here, we describe a new Leydig cell ablation model based on Cre/Lox recombination of mouse Gata4 and Gata6, two genes implicated in the transcriptional regulation of steroidogenesis. The testicular interstitium of adult Gata4(flox/flox) ; Gata6(flox/flox) mice was injected with adenoviral vectors encoding Cre + GFP (Ad-Cre-IRES-GFP) or GFP alone (Ad-GFP). The vectors efficiently and selectively transduced Leydig cells, as evidenced by GFP reporter expression...
October 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28684780/retinoic-acid-affects-lung-adenocarcinoma-growth-by-inducing-differentiation-via-gata6-activation-and-egfr-and-wnt-inhibition
#19
Giovanni Zito, Flores Naselli, Laura Saieva, Stefania Raimondo, Giovanna Calabrese, Claudio Guzzardo, Stefano Forte, Christian Rolfo, Rosalba Parenti, Riccardo Alessandro
A fundamental task in cancer research aims at the identification of new pharmacological therapies that can affect tumor growth. Differentiation therapy might exploit this function not only for hematological diseases, such as acute promyelocytic leukemia (APML) but also for epithelial tumors, including lung cancer. Here we show that Retinoic Acid (RA) arrests in vitro and in vivo the growth of Tyrosine Kinase Inhibitors (TKI) resistant Non Small Cell Lung Cancer (NSCLC). In particular, we found that RA induces G0/G1 cell cycle arrest in TKI resistant NSCLC cells and activates terminal differentiation programs by modulating the expression of GATA6, a key transcription factor involved in the physiological differentiation of the distal lung...
July 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28675489/synergy-between-auraptene-ionizing-radiation-and-anticancer-drugs-in-colon-adenocarcinoma-cells
#20
Mahdi Moussavi, Farhang Haddad, Fatemeh B Rassouli, Mehrdad Iranshahi, Shokouhozaman Soleymanifard
Colorectal cancer is a growing health concern with increasing mortality rates, and resistance to anticancer drugs and radiotherapy is a serious drawback in its treatment. Auraptene is a natural prenyloxycoumarin with valuable anticancer effects. The aim of current study was to determine the synergy between auraptene, ionizing radiation, and chemotherapeutic drugs in colon adenocarcinoma cells for the first time. To do so, HT29 cells were treated with combination of auraptene + cisplatin, + doxorubicin, or + vincristine...
September 2017: Phytotherapy Research: PTR
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