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https://www.readbyqxmd.com/read/28910549/upregulation-of-%C3%AE-enolase-protects-cardiomyocytes-from-phenylephrine-induced-hypertrophy
#1
Si Gao, Xueping Liu, Lihua Wei, Jing Lu, Peiqing Liu
Cardiac hypertrophy often refers to the abnormal growth of heart muscle through a variety of factors. The mechanisms of cardiomyocyte hypertrophy have been extensively investigated using neonatal rat cardiomyocytes treated with phenylephrine. α-Enolase is a glycolytic enzyme with "multifunctional jobs" beyond its catalytic activity. Its possible contribution to cardiac dysfunction remains to be determined. The present study aimed to investigate the change of α-enolase during cardiac hypertrophy and explore its role in this pathological process...
September 14, 2017: Canadian Journal of Physiology and Pharmacology
https://www.readbyqxmd.com/read/28902616/mef2c-loss-of-function-mutation-associated-with-familial-dilated-cardiomyopathy
#2
Fang Yuan, Zhao-Hui Qiu, Xing-Hua Wang, Yu-Min Sun, Jun Wang, Ruo-Gu Li, Hua Liu, Min Zhang, Hong-Yu Shi, Liang Zhao, Wei-Feng Jiang, Xu Liu, Xing-Biao Qiu, Xin-Kai Qu, Yi-Qing Yang
BACKGROUND: The MADS-box transcription factor myocyte enhancer factor 2C (MEF2C) is required for the cardiac development and postnatal adaptation and in mice-targeted disruption of the MEF2C gene results in dilated cardiomyopathy (DCM). However, in humans, the association of MEF2C variation with DCM remains to be investigated. METHODS: The coding regions and splicing boundaries of the MEF2C gene were sequenced in 172 unrelated patients with idiopathic DCM. The available close relatives of the index patient harboring an identified MEF2C mutation and 300 unrelated, ethnically matched healthy individuals used as controls were genotyped for MEF2C...
September 13, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28898757/extracellular-matrix-derived-extracellular-vesicles-promote-cardiomyocyte-growth-and-electrical-activity-in-engineered-cardiac-atria
#3
Minae An, Kihwan Kwon, Junbeom Park, Dong-Ryeol Ryu, Jung-A Shin, Jihee Lee Kang, Ji Ha Choi, Eun-Mi Park, Kyung Eun Lee, Minna Woo, Minsuk Kim
Extracellular matrix (ECM) plays a critical role in the provision of the necessary microenvironment for the proper regeneration of the cardiac tissue. However, specific mechanisms that lead to ECM-mediated cardiac regeneration are not well understood. To elucidate the potential mechanisms, we investigated ultra-structures of the cardiac ECM using electron microscopy. Intriguingly, we observed large quantities of micro-vesicles from decellularized right atria. RNA and protein analyses revealed that these contained exosomal proteins and microRNAs (miRNAs), which we referred to herein as ECM-derived extracellular vesicles (ECM-EVs)...
September 4, 2017: Biomaterials
https://www.readbyqxmd.com/read/28883797/genetic-bases-of-bicuspid-aortic-valve-the-contribution-of-traditional-and-high-throughput-sequencing-approaches-on-research-and-diagnosis
#4
REVIEW
Betti Giusti, Elena Sticchi, Rosina De Cario, Alberto Magi, Stefano Nistri, Guglielmina Pepe
Bicuspid aortic valve (BAV) is a common (0.5-2.0% of general population) congenital heart defect with increased prevalence of aortic dilatation and dissection. BAV has an autosomal dominant inheritance with reduced penetrance and variable expressivity. BAV has been described as an isolated trait or associated with syndromic conditions [e.g., Marfan Marfan syndrome or Loeys-Dietz syndrome (MFS, LDS)]. Identification of a syndromic condition in a BAV patient is clinically relevant to personalize aortic surgery indication...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28864900/expression-of-master-regulatory-genes-of-embryonic-development-in-pancreatic-tumors
#5
L G Kondratyeva, I P Chernov, M V Zinovyeva, E P Kopantzev, E D Sverdlov
The expression level of some important master regulators of embryonic development of the pancreas in the tumor samples of this human organ was determined. We found that the transcription of SOX9, GATA4, PDX1, PTF1a, and HNF1b genes in the tumor samples was reduced as compared to the samples of normal pancreatic tissues, and the KLF5 gene expression in the tumor cells was elevated. We assume that all the studied genes, except KLF5, form a single regulatory module that supports the identity of tumor progenitor cells...
July 2017: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/28863192/e2-er-%C3%AE-inhibit-iso-induced-cardiac-cellular-hypertrophy-by-suppressing-ca2-calcineurin-signaling
#6
Cheng-Yen Tsai, Wei-Wen Kuo, Marthandam Asokan Shibu, Yueh-Min Lin, Chien-Nam Liu, Yi-Hui Chen, Cecilia-Hsuan Day, Chia-Yao Shen, Vijaya Padma Viswanadha, Chih-Yang Huang
Cardiovascular incidences are markedly higher in men than in pre-menstrual women. However, this advantage in women declines with aging and therefore can be correlated with the sex hormone 17β-Estradiol (E2) which is reported to protect heart cells by acting though estrogen receptors (ERs). In this study we have determined the effect of E2/ERβ against ISO induced cellular hypertrophy in H9c2 cardiomyoblast cells. The results confirm that ISO induced cardiac-hypertrophy by elevating the levels of hypertrophy associated proteins, ANP and BNP and further by upregulating p-CaMKII, calcineurin, p-GATA4 and NFATc3 which was correlated with a significant enlargement of the H9c2 cardiomyoblast...
2017: PloS One
https://www.readbyqxmd.com/read/28858485/discovery-of-small-molecules-targeting-the-synergy-of-cardiac-transcription-factors-gata4-and-nkx2-5
#7
Mika J Välimäki, Marja A Tölli, Sini M Kinnunen, Jani Aro, Raisa Serpi, Lotta Pohjolainen, Virpi Talman, Antti Poso, Heikki J Ruskoaho
Transcription factors are pivotal regulators of gene transcription, and many diseases are associated with the deregulation of transcriptional networks. In the heart, the transcription factors GATA4 and NKX2-5 are required for cardiogenesis. GATA4 and NKX2-5 interact physically, and the activation of GATA4, in cooperation with NKX2-5, is essential for stretch-induced cardiomyocyte hypertrophy. Here, we report the identification of four small molecule families that either inhibit or enhance the GATA4-NKX2-5 transcriptional synergy...
September 15, 2017: Journal of Medicinal Chemistry
https://www.readbyqxmd.com/read/28856758/the-gonadal-soma-controls-ovarian-follicle-proliferation-through-gsdf-in-zebrafish
#8
Yi-Lin Yan, Thomas Desvignes, Ruth BreMiller, Catherine Wilson, Danielle Dillon, Samantha High, Bruce Draper, Charles Loren Buck, John Postlethwait
BACKGROUND: Aberrant signaling between germ cells and somatic cells can lead to reproductive disease and depends on diffusible signals, including TGFB-family proteins. The TGFB-family protein Gsdf (gonadal soma derived factor) controls sex determination in some fish and is a candidate for mediating germ cell/soma signaling. RESULTS: Zebrafish expressed gsdf in somatic cells of bipotential gonads and expression continued in ovarian granulosa cells and testicular Sertoli cells...
August 30, 2017: Developmental Dynamics: An Official Publication of the American Association of Anatomists
https://www.readbyqxmd.com/read/28852100/mechanisms-of-stem-cell-based-cardiac-repair-gap-junctional-signaling-promotes-the-cardiac-lineage-specification-of-mesenchymal-stem-cells
#9
Heiko Lemcke, Ralf Gaebel, Anna Skorska, Natalia Voronina, Cornelia Aquilina Lux, Janine Petters, Sarah Sasse, Nicole Zarniko, Gustav Steinhoff, Robert David
Different subtypes of bone marrow-derived stem cells are characterized by varying functionality and activity after transplantation into the infarcted heart. Improvement of stem cell therapeutics requires deep knowledge about the mechanisms that mediate the benefits of stem cell treatment. Here, we demonstrated that co-transplantation of mesenchymal stem cells (MSCs) and hematopoietic stem cells (HSCs) led to enhanced synergistic effects on cardiac remodeling. While HSCs were associated with blood vessel formation, MSCs were found to possess transdifferentiation capacity...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28843068/identification-of-intronic-splice-site-mutations-in-gata4-gene-in-indian-patients-with-congenital-heart-disease
#10
Divya Bose, Vaigundan D, Mitesh Shetty, Krishnappa J, A V M Kutty
Congenital Heart Disease (CHD) is the most common birth defect among congenital anomalies that arise before birth. GATA4 transcription factor plays an important role in foetal heart development. Mutational analysis of GATA4 gene in CHD patients revealed five known heterozygous mutations (p.T355S, p.S377G, p.V380M, p.P394T and p.D425N) identified in exons 5 and 6 regions and fifteen intronic variants in the non-coding regions (g.76885T>C/Y,g.76937G>S, g.78343G>R, g.83073T>Y, g.83271C>A/M, g.83318G>K, g...
August 18, 2017: Mutation Research
https://www.readbyqxmd.com/read/28836411/evidence-of-oocyte-polarity-in-bovine-implications-for-intracytoplasmic-sperm-injection-and-somatic-cell-nuclear-transfer
#11
Seyed Morteza Hosseini, Fariba Moulavi, Nima TanhaieVash, Naser Shams-Esfandabadi, Mohammad Hossein Nasr-Esfahani, Abolfazl Shirazi
OBJECTIVES: We recently demonstrated spatial regionalization of maternal transcripts and proteins within unfertilized ovine oocyte. Here, we investigated the likelihood of oocyte polarity for the first time in bovine. MATERIALS AND METHODS: In this experimental study, in vitro matured bovine oocytes were used for manual bisection [into oocyte halve that were near-to (HNS) and far-from (FS) spindle] or trisection [into MII-spindle (S), the spindle-side half (NS), and the distal half unassociated with the spindle (FS)]...
October 2017: Cell Journal
https://www.readbyqxmd.com/read/28834610/nestin-expression-is-dynamically-regulated-in-cardiomyocytes-during-embryogenesis
#12
Vanessa Hertig, Adrianna Matos Nieves, Vidu Garg, Louis Villeneuve, Maya Mamarbachi, Laurie Caland, Angelino Calderone
The transcriptional factors implicated in the expression of the intermediate filament protein nestin in cardiomyocytes during embryogenesis remain undefined. In the heart of 9,5-10,5 day embryonic mice, nestin staining was detected in atrial and ventricular cardiomyocytes and a subpopulation co-expressed Tbx5. At later stages of development, nestin immunoreactivity in cardiomyocytes gradually diminished and was absent in the heart of 17,5 day embryonic mice. In the heart of wild type 11,5 day embryonic mice, 54 ± 7% of the trabeculae expressed nestin and the percentage was significantly increased in the hearts of Tbx5(+/-) and Gata4(+/-) embryos...
August 23, 2017: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/28825623/single-construct-polycistronic-doxycycline-inducible-vectors-improve-direct-cardiac-reprogramming-and-can-be-used-to-identify-the-critical-timing-of-transgene-expression
#13
Tomohiko C Umei, Hiroyuki Yamakawa, Naoto Muraoka, Taketaro Sadahiro, Mari Isomi, Sho Haginiwa, Hidenori Kojima, Shota Kurotsu, Fumiya Tamura, Rina Osakabe, Hidenori Tani, Kaori Nara, Hiroyuki Miyoshi, Keiichi Fukuda, Masaki Ieda
Direct reprogramming is a promising approach in regenerative medicine. Overexpression of the cardiac transcription factors Gata4, Mef2c, and Tbx5 (GMT) or GMT plus Hand2 (GHMT) directly reprogram fibroblasts into cardiomyocyte-like cells (iCMs). However, the critical timing of transgene expression and the molecular mechanisms for cardiac reprogramming remain unclear. The conventional doxycycline (Dox)-inducible temporal transgene expression systems require simultaneous transduction of two vectors (pLVX-rtTA/pLVX-cDNA) harboring the reverse tetracycline transactivator (rtTA) and the tetracycline response element (TRE)-controlled transgene, respectively, leading to inefficient cardiac reprogramming...
August 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28810055/impact-of-mir-26b-on-cardiomyocyte-differentiation-in-p19-cells-through-regulating-canonical-non-canonical-wnt-signalling
#14
Duo Wang, Chang Liu, Yumei Wang, Wenjing Wang, Kang Wang, Xiujuan Wu, Zhigang Li, Cuimei Zhao, Li Li, Luying Peng
BACKGROUND AND OBJECTIVES: The control of cardiomyocyte differentiation is tightly linked to microRNAs (miRNAs), which have been emerging as important players in heart development. However, the regulation mechanisms mediated by miRNAs in early heart development remains speculative. Here, we evaluated the impact of miR-26b during the progression of cardiomyocyte differentiation from the P19 cell line. MATERIALS AND METHODS: The overexpression of miR-26b in P19 cells was performed by transduction with lentivirus vector...
August 15, 2017: Cell Proliferation
https://www.readbyqxmd.com/read/28805231/the-histone-methyltransferase-mixed-lineage-leukemia-mll-3-may-play-a-potential-role-on-clinical-dilated-cardiomyopathy
#15
Ding-Sheng Jiang, Xin Yi, Rui Li, Yun-Shu Su, Jing Wang, Min-Lai Chen, Li-Gang Liu, Min Hu, Cai Cheng, Ping Zheng, Xue-Hai Zhu, Xiang Wei
Histone modifications play a critical role in the pathological processes of dilated cardiomyopathy (DCM). While the role and expression pattern of histone methyltransferases (HMTs), especially mixed lineage leukemia (MLL) families on DCM are unclear. To this end, twelve normal and fifteen DCM heart samples were included in the present study. A murine cardiac remodelling model was induced by transverse aortic constriction (TAC). Real-time PCR was performed to detect the expression levels of MLL families in the mouse and human left ventricles...
August 9, 2017: Molecular Medicine
https://www.readbyqxmd.com/read/28802159/increased-%C3%AE-catenin-accumulation-and-nuclear-translocation-are-associated-with-concentric-hypertrophy-in-cardiomyocytes
#16
Cheng-Yu Lee, Wei Wen Kuo, Rathinasamy Baskaran, Cecilia Hsuan Day, Pei Ying Pai, Chao Hung Lai, Yu-Feng Chen, Ray-Jade Chen, Viswanadha Vijaya Padma, Chih Yang Huang
Defective Wnt/β-Catenin signaling, activated under various pathological conditions, can result in cardiac and vascular abnormalities. In the present study, the possible role of β-catenin over expression during cardiac hypertrophy was investigated. Ten samples from hearts of human patients with acute infarction, and granulation tissue from 20 patients and 10 from normal ones were collected in order to investigate roles of β-catenin in cardiac hypertrophy. H9c2 cardiomyoblast cells and Wistar rat primary neonatal cardiomyocytes were overexpressed with β-catenin...
July 12, 2017: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
https://www.readbyqxmd.com/read/28801055/therapy-with-mesenchymal-stromal-cells-or-conditioned-medium-reverse-cardiac-alterations-in-a-high-fat-diet-induced-obesity-model
#17
P S Daltro, B C Barreto, P G Silva, P Chenaud Neto, P H F Sousa Filho, D Santana Neta, G B Carvalho, D N Silva, B D Paredes, A C de Alcantara, L A R Freitas, R D Couto, R R Santos, B S F Souza, M B P Soares, S G Macambira
BACKGROUND: Obesity is associated with numerous cardiac complications, including arrhythmias, cardiac fibrosis, remodeling and heart failure. Here we evaluated the therapeutic potential of mesenchymal stromal cells (MSCs) and their conditioned medium (CM) to treat cardiac complications in a mouse model of high-fat diet (HFD)-induced obesity. METHODS: After obesity induction and HFD withdrawal, obese mice were treated with MSCs, CM or vehicle. Cardiac function was assessed using electrocardiography, echocardiography and treadmill test...
August 8, 2017: Cytotherapy
https://www.readbyqxmd.com/read/28795648/human-embryonic-stem-cell-derived-cardiomyocytes-self-arrange-with-areas-of-different-subtypes-during-differentiation
#18
Maj Linea Vestergaard, Søren J Grubb, Karen Koefod Rasmussen, Zoe Anderson-Jenkins, Kristina Grunnet-Lauridsen, Kristine Calloe, Christian Clausen, Søren T Christensen, Kjeld Møllgård, Claus Yding Andersen
The derivation of functional cardiomyocytes (CMs) from human embryonic stem cells (hESC) represents a unique way of studying human cardiogenesis, including the development of CM subtypes. In this study, we investigated the development and organization of CMs derived from hESCs (hESC-CMs) and examined how the expression of CM subtypes correspond to human in vivo cardiogenesis. Beating clusters were used to determine cardiac differentiation, which was evaluated by the expression of cardiac genes GATA4 and TNNT2 and subcellular localization of GATA4 and NKX2...
August 10, 2017: Stem Cells and Development
https://www.readbyqxmd.com/read/28791052/inhibition-of-histone-methyltransferase-histone-deacetylase-and-%C3%AE-catenin-synergistically-enhance-the-cardiac-potential-of-bone-marrow-cells
#19
Jinpu Yang, Keerat Kaur, John G Edwards, Carol A Eisenberg, Leonard M Eisenberg
Previously, we reported that treatment with the G9a histone methyltransferase inhibitor BIX01294 causes bone marrow mesenchymal stem cells (MSCs) to exhibit a cardiocompetent phenotype, as indicated by the induction of the precardiac markers Mesp1 and brachyury. Here, we report that combining the histone deacetylase inhibitor trichostatin A (TSA) with BIX01294 synergistically enhances MSC cardiogenesis. Although TSA by itself had no effect on cardiac gene expression, coaddition of TSA to MSC cultures enhanced BIX01294-induced levels of Mesp1 and brachyury expression 5...
2017: Stem Cells International
https://www.readbyqxmd.com/read/28789786/more-strata-and-another-gata-novel-roles-for-gata4-in-postinfarct-myocardial-repair
#20
EDITORIAL
Leora B Balsam
No abstract text is available yet for this article.
July 18, 2017: Journal of Thoracic and Cardiovascular Surgery
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