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https://www.readbyqxmd.com/read/28490091/physico-chemical-characterization-of-dairy-gel-obtained-by-a-proteolytic-extract-from-calotropis-procera-a-comparison-with-chymosin
#1
Issa Ado Rayanatou, ElHadji Gounga Mahamadou, Gilles Garric, Marielle Harel-Oger, Arlette Leduc, Julien Jardin, Valérie Briard-Bion, Chantal Cauty, Hassane Adakal, Jean François Grongnet, Frédéric Gaucheron
Chymosin is the major enzyme used in cheesemaking but latex enzymes are also used. The aim of this work was to characterize the composition and the structure of dairy gel obtained by an extract of Calotropis procera leaves in comparison with those obtained by chymosin. The biochemical and mineral compositions of the curds and the cheese yields obtained by using Calotropis procera extract or chymosin were relatively similar. Quantitative and qualitative evaluations of proteolysis after milk coagulation, determined by the non-protein nitrogen content and chromatography coupled to mass spectrometry, indicated that Calotropis procera extract was more proteolytic than chymosin and that κ-casein was proteolyzed...
October 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28437539/safety-and-efficacy-of-antiviral-therapy-for-prevention-of-cytomegalovirus-reactivation-in-immunocompetent-critically-ill-patients-a-randomized-clinical-trial
#2
Nicholas J Cowley, Andrew Owen, Sarah C Shiels, Joanne Millar, Rebecca Woolley, Natalie Ives, Husam Osman, Paul Moss, Julian F Bion
Importance: Latent cytomegalovirus (CMV) infection is present in more than half the adult population, and a viral reactivation (ie, when the virus becomes measurable in body fluids such as blood) can occur in up to one-third of these individuals during episodes of critical illness. Objective: To determine whether antiviral therapy is safe and effective for preventing CMV reactivation in a general population of critically ill patients. Design, Setting, and Participants: A single-center, open-label, randomized, controlled clinical trial recruited 124 CMV-seropositive patients undergoing mechanical ventilation for at least 24 hours in the intensive care unit between January 1, 2012, and January 31, 2014...
April 24, 2017: JAMA Internal Medicine
https://www.readbyqxmd.com/read/28406170/identification-of-proteins-involved-in-the-anti-inflammatory-properties-of-propionibacterium-freudenreichii-by-means-of-a-multi-strain-study
#3
Stéphanie-Marie Deutsch, Mahendra Mariadassou, Pierre Nicolas, Sandrine Parayre, Rozenn Le Guellec, Victoria Chuat, Vincent Peton, Caroline Le Maréchal, Julien Burati, Valentin Loux, Valérie Briard-Bion, Julien Jardin, Coline Plé, Benoît Foligné, Gwénaël Jan, Hélène Falentin
Propionibacterium freudenreichii, a dairy starter, can reach a population of almost 10(9) propionibacteria per gram in Swiss-type cheese at the time of consumption. Also consumed as a probiotic, it displays strain-dependent anti-inflammatory properties mediated by surface proteins that induce IL-10 in leukocytes. We selected 23 strains with varied anti-inflammatory potentials in order to identify the protein(s) involved. After comparative genomic analysis, 12 of these strains were further analysed by surface proteomics, eight of them being further submitted to transcriptomics...
April 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28398923/the-association-between-visiting-intensivists-and-icu-outcomes
#4
Tony Whitehouse, James Hodson, Philip Pemberton, Tonny Veenith, Catherine Snelson, Julian Bion, Gordon D Rubenfeld
OBJECTIVES: We hypothesized that intensivists unfamiliar with an ICU team and the context of that ICU would affect patient outcomes. We examined differences in mortality when ICU patients were admitted under intensivists routinely working in that ICU and compared with those admitted by intensivists familiar with an ICU elsewhere in the same hospital. DESIGN, SETTINGS, AND PATIENTS: A 5-year natural experimental crossover study involving patients admitted to four ICUs in a large U...
April 10, 2017: Critical Care Medicine
https://www.readbyqxmd.com/read/28385913/the-weekend-effect-in-acute-medicine-a-protocol-for-a-team-based-ethnography-of-weekend-care-for-medical-patients-in-acute-hospital-settings
#5
Carolyn Tarrant, Elizabeth Sutton, Emma Angell, Cassie P Aldridge, Amunpreet Boyal, Julian Bion
INTRODUCTION: It is now well-recognised that patients admitted to hospital on weekends are at higher risk of death than those admitted during weekdays. However, the causes of this 'weekend effect' are poorly understood. Some contend that there is a deficit of medical staff on weekends resulting in poorer quality care, whereas others find that patients admitted to hospital on weekends are sicker and therefore at higher risk of adverse outcomes. Clarifying the causal pathway is clearly important in order to identify effective solutions...
April 5, 2017: BMJ Open
https://www.readbyqxmd.com/read/28322090/the-chemical-inducer-bth-benzothiadiazole-and-root-colonization-by-mycorrhizal-fungi-glomus-spp-trigger-resistance-against-white-rot-sclerotinia-sclerotiorum-in-sunflower
#6
Rita Bán, Gellért Baglyas, Ferenc Virányi, Balázs Barna, Katalin Posta, József Kiss, Katalin Körösi
White rot caused by Sclerotinia sclerotiorum (SS) is one of the most devastating plant diseases of sunflower. Controlling this pathogen by available tools hardly result in acceptable control. The aim of this study was to elucidate the effects of plant resistance inducers, BTH (benzothiadiazole in Bion 50 WG) and arbuscular mycorrhizal fungi (AMF) on disease development of white rot in three sunflower genotypes. Defence responses were characterized by measuring the disease severity and identifying cellular/histological reactions (e...
March 2017: Acta Biologica Hungarica
https://www.readbyqxmd.com/read/28243916/myeloid-precursors-in-the-bone-marrow-of-mice-after-a-30-day-space-mission-on-a-bion-m1-biosatellite
#7
E V Sotnezova, E A Markina, E R Andreeva, L B Buravkova
The content of myeloid stem CFU in bone marrow karyocytes from the tibial bone of C57Bl/6 mice was evaluated after a 30-day Bion-M1 pace flight/ground control experiment and subsequent 7-day recovery period. After the space flight, we observed a significant decrease in the number of erythroid progenitors in the bone marrow, including common myeloid precursor - granulocyte, erythrocyte, monocyte/macrophage, megakaryocyte CFU. After 7-day readaptation, CFU level in flight animals did not recover completely. In the ground control, the count of erythroid burst-forming units was higher than in vivarium animals...
February 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28211036/unconscious-phantasy-as-a-structural-principle-and-organizer-of-mental-life-the-evolution-of-a-concept-from-freud-to-klein-and-some-of-her-successors
#8
Heinz Weiss
Starting with Freud's discovery of unconscious phantasy as a means of accessing his patients' internal world, the author discusses the evolution of the concept in the work of Melanie Klein and some of her successors. Whereas Freud sees phantasy as a wish fulfilling imagination, dominated by primary process functioning and kept apart from reality testing, Klein understands phantasies as a structural function and organizer of mental life. From their very beginnings they involve object relations and gradually evolve from primitive body-near experiences to images and symbolic representations...
February 17, 2017: International Journal of Psycho-analysis
https://www.readbyqxmd.com/read/28181811/a-new-delphi-feature-for-modeling-electrostatic-potential-around-proteins-role-of-bound-ions-and-implications-for-zeta-potential
#9
Arghya Chakravorty, Zhe Jia, Lin Li, Emil Alexov
A new feature of the popular software DelPhi is developed and reported, allowing for computing the surface averaged electrostatic potential (SAEP) of macromolecules. The user is given the option to specify the distance from the van der Waals surface where the electrostatic potential will be outputted. In conjunction with DelPhiPKa and the BION server, the user can adjust the charges of titratable groups according to specific pH values, and add explicit ions bound to the macromolecular surface. This approach is applied to a set of four proteins with "experimentally" delivered zeta (ζ)-potentials at different pH values and salt concentrations...
February 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28165164/synthesis-of-unique-flowerlike-bi2-o2-oh-no3-hierarchical-microstructures-with-high-surface-area-and-superior-photocatalytic-performance
#10
Qiaofeng Han, Jiawei Pang, Xin Wang, Xiaodong Wu, Junwu Zhu
Unique flowerlike Bi2 O2 (OH)(NO3 ) (denoted as BION) hierarchical microstructures assembled by ultrathin nanosheets were hydrothermally synthesized from incomplete hydrolysis of anhydrous bismuth nitrate (Bi(NO3 )3 ) after adsorption of glacial acetic acid (HAc). The structure, composition, and optical properties of the products were characterized by X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), UV/Vis spectroscopy, etc. The as-prepared flowerlike BION possessed an ultra-high surface area and thus exhibited exceptional photocatalytic activity for rhodamine B (RhB) degradation under UV light irradiation with an efficiency of about 17; 6 and 2...
February 6, 2017: Chemistry: a European Journal
https://www.readbyqxmd.com/read/28144642/genetic-distances-in-three-ascidian-species-determined-by-pcr-technique
#11
Jong-Man Yoon
Seven oligonucleotides primers were shown to generate the shared loci, specific loci, unique shared loci to each species and shared loci by the three species which could be obviously scored. In the present study, 7 oligonucleotides primers produced 401 total loci in the Styela clava (SC) species, 390 in the Halocynthia roretzi (HR) and 434 in the Styela plicata (SP), respectively. Seven oligonucleotides primers generated 275 specific loci in the SC, 341 in the HR and 364 in the SP species, respectively. The oligonucleotides primer BION-23 generated 28 unique loci to each species in the SP species...
December 2016: Balsaeng'gwa Saengsig
https://www.readbyqxmd.com/read/28135156/dream-images-and-creation
#12
Céline Masson, Silke Schauder, Simone Korff Sausse
This article links contemporary psychoanalytic theories of the dream, especially Bion's, with the work of the American video artist Bill Viola, who is deeply influenced by altered states of consciousness and produces images of dreamlike quality. We discuss the oneiric and infantile roots of creativity and artistic inspiration, finally taking Viola's monumental artwork The Passing (1991) as paradigmatic of the artist's aesthetic and philosophical elaboration of the relationship between life and death.
February 2017: Psychoanalytic Review
https://www.readbyqxmd.com/read/28091925/changes-in-the-neurochemical-composition-of-motor-neurons-of-the-spinal-cord-in-mice-under-conditions-of-space-flight
#13
V V Porseva, V V Shilkin, A A Strelkov, I B Krasnov, P M Masliukov
Expression of choline acetyltransferase, 200-kDa neurofilament protein, 28-kDa calbindin, neuronal NO synthase, caspase 3, and Ki-67 in the motor neurons of spinal cord segments T3-T5 in male C57Bl/6 mice after 30-day space flight in the Bion-M1 biosatellite was studied by immunohistochemical methods. Under conditions space flight, the size of motoneurons increased, the number of neurons containing choline acetyltransferase and neurofilaments, decreased, and the number of calbindin-positive neurons increased; motoneurons, expressing neuronal NO synthase and caspase 3 appeared, while Ki-67 was not detected...
January 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28088578/the-effect-of-long-term-hindlimb-unloading-on-the-expression-of-risk-neurogenes-encoding-elements-of-serotonin-dopaminergic-systems-and-apoptosis-comparison-with-the-effect-of-actual-spaceflight-on-mouse-brain
#14
E A Kulikova, V A Kulikov, N A Sinyakova, A V Kulikov, N K Popova
The study of spaceflight effects on the brain is technically complex concern; complicated by the problem of applying an adequate ground model. The most-widely used experimental model to study the effect of microgravity is the tail-suspension hindlimb unloading model; however, its compliance with the effect of actual spaceflight on the brain is still unclear. We evaluated the effect of one month hindlimb unloading on the expression of genes related to the brain neuroplasticity-brain neutotrophic factors (Gdnf, Cdnf), apoptotic factors (Bcl-xl, Bax), serotonin- and dopaminergic systems (5-HT2A, Maoa, Maob, Th, D1r, Comt), and compared the results with the data obtained on mice that spent one month in spaceflight on Russian biosatellite Bion-M1...
February 15, 2017: Neuroscience Letters
https://www.readbyqxmd.com/read/28077852/growth-and-turbulence-in-the-container-contained-bion-s-continuing-legacy-edited-by-howard-b-levine-and-lawrence-j-brown-routledge-2013-323pp
#15
Virginia Rachmani
No abstract text is available yet for this article.
December 2016: American Journal of Psychoanalysis
https://www.readbyqxmd.com/read/28076365/gene-expression-profiling-in-slow-type-calf-soleus-muscle-of-30-days-space-flown-mice
#16
Guido Gambara, Michele Salanova, Stefano Ciciliot, Sandra Furlan, Martina Gutsmann, Gudrun Schiffl, Ute Ungethuem, Pompeo Volpe, Hanns-Christian Gunga, Dieter Blottner
Microgravity exposure as well as chronic disuse are two main causes of skeletal muscle atrophy in animals and humans. The antigravity calf soleus is a reference postural muscle to investigate the mechanism of disuse-induced maladaptation and plasticity of human and rodent (rats or mice) skeletal musculature. Here, we report microgravity-induced global gene expression changes in space-flown mouse skeletal muscle and the identification of yet unknown disuse susceptible transcripts found in soleus (a mainly slow phenotype) but not in extensor digitorum longus (a mainly fast phenotype dorsiflexor as functional counterpart to soleus)...
2017: PloS One
https://www.readbyqxmd.com/read/27871948/vestibular-cerebellum-of-thick-toed-geckos-chondrodactylus-turnery-gray-1864-and-c57-bl6n-mice-after-the-long-term-space-flight-on-the-biosatellite-bion-m1
#17
Proshchina Alexandra, Kharlamova Anastasia, Barabanov Valeriy, Gulimova Victoria, Saveliev Sergey
The aim of this study was to estimate the effects of long-term space flights on neuronal and glial cells of the vestibular cerebellum of C57/BL6N mice and thick-toed geckos (Chondrodactylus turnery GRAY, 1864). The cerebella from 26 mice and 13 geckos were used in this study. Ten mice and five geckos were flown aboard the BION-M1 biosatellite. The other animals were used as controls. We used immunohistochemical techniques and classical histological method to reveal cell types in the vestibular cerebellum. Nonspecific pathomorphological changes in the Purkinje cells (such as chromatolysis, vacuolization and hyperchromatosis) were observed in the flight groups...
January 2017: Journal of Chemical Neuroanatomy
https://www.readbyqxmd.com/read/27865996/in-vivo-effects-of-metal-ions-on-conformation-and-mechanical-performance-of-silkworm-silks
#18
Xin Wang, Yi Li, Qingsong Liu, Quanmei Chen, Qingyou Xia, Ping Zhao
BACKGROUND: The mechanism of silk fiber formation is of particular interest. Although in vitro evidence has shown that metal ions affect conformational transitions of silks, the in vivo effects of metal ions on silk conformations and mechanical performance are still unclear. METHODS: This study explored the effects of metal ions on silk conformations and mechanical properties of silk fibers by adding K(+) and Cu(2+) into the silk fibroin solutions or injecting them into the silkworms...
March 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27860373/investigation-of-methane-oxidation-reactions-over-a-dual-bed-catalyst-system-using-18-o-labelled-drifts-coupling
#19
Melissandre Richard, Daniel Duprez, Nicolas Bion, Fabien Can
Low loading Pd-supported (0.2 wt % Pd) Y-stabilized zirconia (YSZ) and LaMnO3 (LM) perovskite were associated to study the partial oxidation of methane using labelled (18) O2 in the gas phase. Synthesis gas production was demonstrated to occur through an indirect reaction in which oxygen is first consumed in the total methane combustion. A Mars-van Krevelen mechanism was observed over Pd/YSZ at 425 °C to yield C(16) O2 and C(16) O. A significant enhancement of the Pd/YSZ catalyst activity was achieved by the association of LM-Pd/YSZ in a dual catalyst bed, resulting in a significant increase of the oxidation rate...
November 9, 2016: ChemSusChem
https://www.readbyqxmd.com/read/27852320/a-complex-endeavour-an-ethnographic-study-of-the-implementation-of-the-sepsis-six-clinical-care-bundle
#20
Carolyn Tarrant, Barbara O'Donnell, Graham Martin, Julian Bion, Alison Hunter, Kevin D Rooney
BACKGROUND: Implementation of the 'Sepsis Six' clinical care bundle within an hour of recognition of sepsis is recommended as an approach to reduce mortality in patients with sepsis, but achieving reliable delivery of the bundle has proved challenging. There remains little understanding of the barriers to reliable implementation of bundle components. We examined frontline clinical practice in implementing the Sepsis Six. METHODS: We conducted an ethnographic study in six hospitals participating in the Scottish Patient Safety Programme Sepsis collaborative...
November 16, 2016: Implementation Science: IS
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