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https://www.readbyqxmd.com/read/28926817/safety-of-bacillus-thuringiensis-cry1c-protein-for-daphnia-magna-based-on-different-functional-traits
#1
Yi Chen, Yan Yang, Haojun Zhu, Jörg Romeis, Yunhe Li, Yufa Peng, Xiuping Chen
Cry1C is a Bacillus thuringiensis (Bt) insecticidal protein and it can be produced by transgenic rice lines developed in China. Cladocera species are common aquatic arthropods that may be exposed to insecticidal proteins produced in Bt-transgenic plants through ingestion of pollen or crop residues in water. As the cladoceran Daphnia magna plays an important role in the aquatic food chain, it is important to assess the possible effects of Bt crops to this species. To evaluate the safety of the Cry1C protein for D...
September 16, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28924217/global-transcriptome-analysis-and-identification-of-genes-involved-in-nutrients-accumulation-during-seed-development-of-rice-tartary-buckwheat-fagopyrum-tararicum
#2
Juan Huang, Jiao Deng, Taoxiong Shi, Qijiao Chen, Chenggang Liang, Ziye Meng, Liwei Zhu, Yan Wang, Fengli Zhao, Shizhou Yu, Qingfu Chen
Tartary buckwheat seeds are rich in various nutrients, such as storage proteins, starch, and flavonoids. To get a good knowledge of the transcriptome dynamics and gene regulatory mechanism during the process of seed development and nutrients accumulation, we performed a comprehensive global transcriptome analysis using rice tartary buckwheat seeds at different development stages, namely pre-filling stage, filling stage, and mature stage. 24 819 expressed genes, including 108 specifically expressed genes, and 11 676 differentially expressed genes (DEGs) were identified...
September 18, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28923810/an-innovative-community-based-model-for-improving-preventive-care-in-rural-counties
#3
Zsolt J Nagykaldi, Dewey Scheid, Daniel Zhao, Bhawani Mishra, Tracy Greever-Rice
OBJECTIVE: This quasi-experimental pilot study aimed to implement and evaluate a sustainable, rural community-based patient outreach model for preventive care provided through primary care practices (PCPs) located in a rural county in Oklahoma. A Wellness Coordinator (WC) working with PCPs, the county health department, the county hospital, and a health information exchange (HIE) organization helped county residents receive evidence-based preventive services. METHODS: The WC used a community wellness registry connected to electronic medical records via HIE and called patients at the county level based on PCP-prioritized and tailored protocols...
September 2017: Journal of the American Board of Family Medicine: JABFM
https://www.readbyqxmd.com/read/28922774/crop-model-assisted-phenomics-and-genome-wide-association-study-for-climate-adaptation-of-indica-rice-1-phenology
#4
Michael Dingkuhn, Richard Pasco, Julie M Pasuquin, Jean Damo, Jean-Christophe Soulié, Louis-Marie Raboin, Julie Dusserre, Abdoulaye Sow, Baboucarr Manneh, Suchit Shrestha, Alpha Balde, Tobias Kretzschmar
Phenology and time of flowering are crucial determinants of rice adaptation to climate variation. A previous study characterized flowering responses of 203 diverse indica rices (the ORYTAGE panel) to ten environments in Senegal (six sowing dates) and Madagascar (two years and two altitudes) under irrigation in the field. This study used the physiological phenology model RIDEV V2 to heuristically estimate component traits of flowering such as cardinal temperatures (base temperature (Tbase) and optimum temperature), basic vegetative phase, photoperiod sensitivity and cold acclimation, and to conduct a genome-wide association study for these traits using 16 232 anonymous single-nucleotide polymorphism (SNP) markers...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922773/crop-model-assisted-phenomics-and-genome-wide-association-study-for-climate-adaptation-of-indica-rice-2-thermal-stress-and-spikelet-sterility
#5
Michael Dingkuhn, Richard Pasco, Julie Mae Pasuquin, Jean Damo, Jean-Christophe Soulié, Louis-Marie Raboin, Julie Dusserre, Abdoulaye Sow, Baboucarr Manneh, Suchit Shrestha, Tobias Kretzschmar
Low night and high day temperatures during sensitive reproductive stages cause spikelet sterility in rice. Phenotyping of tolerance traits in the field is difficult because of temporal interactions with phenology and organ temperature differing from ambient. Physiological models can be used to separate these effects. A 203-accession indica rice diversity panel was phenotyped for sterility in ten environments in Senegal and Madagascar and climate data were recorded. Here we report on sterility responses while a companion study reported on phenology...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922766/altered-accumulation-of-osa-mir171b-contributes-to-rice-stripe-virus-infection-by-regulating-disease-symptoms
#6
Aizi Tong, Quan Yuan, Shu Wang, Jiejun Peng, Yuwen Lu, Hongying Zheng, Lin Lin, Hairu Chen, Yifu Gong, Jianping Chen, Fei Yan
Viral infection affects the pattern of plant miRNA expression. It has been presumed that reduction of miR171 and several other miRNAs influences viral symptoms in plants. We here experimentally demonstrate the association of osa-miR171b with rice stripe virus (RSV) symptoms in rice. Inhibition of osa-miR171b caused stunting with reduced chlorophyll content in leaves similar to viral symptoms. Overexpression of osa-miR171b by an artificial miRNA extended vegetative growth and enhanced chlorophyll accumulation in leaves...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922754/rice-phytochrome-interacting-factor-like1-ospil1-is-involved-in-the-promotion-of-chlorophyll-biosynthesis-through-feed-forward-regulatory-loops
#7
Yasuhito Sakuraba, Eun-Young Kim, Su-Hyun Han, Weilan Piao, Gynheung An, Daisuke Todaka, Kazuko Yamaguchi-Shinozaki, Nam-Chon Paek
In phototrophic plants, the highly conserved and tightly regulated process of chlorophyll (Chl) biosynthesis comprises multi-step reactions involving more than 15 enzymes. Since the efficiency of Chl biosynthesis strongly affects plant productivity, understanding the underlying regulatory mechanisms in crop plants can be useful for strategies to increase grain and biomass yields. Here, we show that rice (Oryza sativa) Phytochrome-Interacting Factor-Like1 (OsPIL1), a basic helix-loop-helix transcription factor, promotes Chl biosynthesis...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922752/current-understanding-of-the-pathways-of-flavonoid-biosynthesis-in-model-and-crop-plants
#8
Takayuki Tohge, Leonardo Perez de Souza, Alisdair R Fernie
Flavonoids are a signature class of secondary metabolites formed from a relatively simple collection of scaffolds. They are extensively decorated by chemical reactions including glycosylation, methylation, and acylation. They are present in a wide variety of fruits and vegetables and as such in Western populations it is estimated that 20-50 mg of flavonoids are consumed daily per person. In planta they have demonstrated to contribute to both flower color and UV protection. Their consumption has been suggested to presenta wide range of health benefits...
July 10, 2017: Journal of Experimental Botany
https://www.readbyqxmd.com/read/28922748/cesium-uptake-by-rice-roots-largely-depends-upon-a-single-gene-hak1-which-encodes-a-potassium-transporter
#9
Hiroki Rai, Saki Yokoyama, Namiko Satoh-Nagasawa, Jun Furukawa, Takiko Nomi, Yasuka Ito, Shigeto Fujimura, Hidekazu Takahashi, Ryuichiro Suzuki, ELMannai Yousra, Akitoshi Goto, Shinichi Fuji, Shin-Ichi Nakamura, Takuro Shinano, Nobuhiro Nagasawa, Hiroetsu Wabiko, Hiroyuki Hattori
Incidents at the Fukushima and Chernobyl nuclear power stations have resulted in widespread environmental contamination by radioactive nuclides. Among them, 137cesium has a 30 year half-life, and its persistence in soil raises serious food security issues. It is therefore important to prevent plants, especially crop plants, from absorbing radiocesium. In Arabidopsis thaliana, cesium ions are transported into root cells by several different potassium transporters such as high-affinity K+ transporter 5 (AtHAK5)...
September 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28922746/ectopic-expression-of-winding-1-leads-to-asymmetrical-distribution-of-auxin-and-a-spiral-phenotype-in-rice
#10
Ming-Lung Cheng, Shuen-Fang Lo, An-Shan Hsiao, Ya-Fang Hong, Su-May Yu, Tuan-Hua David Ho
Ectopic expression of the rice WINDING 1 (WIN1) gene leads to a spiral phenotype only in shoots but not in roots. Rice WIN1 belongs to a specific class of proteins in cereal plants containing a Bric-a-Brac/Tramtrack/Broad (BTB) complex, a non-phototropic hypocotyl 3 (NPH3) domain and a coiled-coil motif. The WIN1 protein is predominantly localized to the plasma membrane, but is also co-localized to plasmodesmata, where it exhibits a punctate pattern. It is observed that WIN1 is normally expressed in roots and the shoot-root junction, but not in the rest of shoots...
September 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28922744/sugar-transporters-in-plants-new-insights-and-discoveries
#11
Benjamin T Julius, Kristen A Leach, Thu M Tran, Rachel A Mertz, David M Braun
Carbohydrate partitioning is the process of carbon assimilation and distribution from source tissues, such as leaves, to sink tissues, such as stems, roots and seeds. Sucrose, the primary carbohydrate transported long distance in many plant species, is loaded into the phloem and unloaded into distal sink tissues. However, many factors, both genetic and environmental, influence sucrose metabolism and transport. Therefore, understanding the function and regulation of sugar transporters and sucrose metabolic enzymes is key to improving agriculture...
September 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28922554/performance-comparison-of-dispersive-solid-phase-extraction-and-multiplug-filtration-cleanup-methods-for-the-determination-of-tefuryltrione-in-plant-and-environmental-samples-using-uhplc-ms-ms
#12
Yang Yu, Yan Tao, Fengshou Dong, Jun Xu, Xingang Liu, Xiaohu Wu, Mingshan Ji, Yongquan Zheng
The detection frequencies of tefuryltrione, a new type of 4-hydroxyphenyl-pyruvate dioxygenase inhibitor herbicide, are rarely reported, probably because of the paucity of analytical methods. Herein, an effective and sensitive analytical method has been developed to detect tefuryltrione in vegetables (tomato and cucumber), cereals (rice and corn), soil and water by ultra high performance liquid chromatography coupled with tandem mass spectrometry. Comparisons of the performances of dispersive solid-phase extraction and multiplug filtration cleanup methods were carried out for tefuryltrione in complex matrices...
September 18, 2017: Journal of Separation Science
https://www.readbyqxmd.com/read/28922368/a-tree-of-life-based-on-ninety-eight-expressed-genes-conserved-across-diverse-eukaryotic-species
#13
Pawan Kumar Jayaswal, Vivek Dogra, Asheesh Shanker, Tilak Raj Sharma, Nagendra Kumar Singh
Rapid advances in DNA sequencing technologies have resulted in the accumulation of large data sets in the public domain, facilitating comparative studies to provide novel insights into the evolution of life. Phylogenetic studies across the eukaryotic taxa have been reported but on the basis of a limited number of genes. Here we present a genome-wide analysis across different plant, fungal, protist, and animal species, with reference to the 36,002 expressed genes of the rice genome. Our analysis revealed 9831 genes unique to rice and 98 genes conserved across all 49 eukaryotic species analysed...
2017: PloS One
https://www.readbyqxmd.com/read/28921303/registry-study-to-assess-hair-loss-prevention-with-the-penguin-cold-cap-in-breast-cancer-patients-receiving-chemotherapy
#14
Brooke A Rice, Elizabeth S Ver Hoeve, Amy N DeLuca, Laura J Esserman, Hope S Rugo, Michelle E Melisko
PURPOSE: Chemotherapy-induced alopecia is a distressing side effect of cancer treatment. The aim of this registry study was to assess efficacy and tolerability of scalp hypothermia using Penguin Cold Caps (Penguin) in breast cancer patients. METHODS: Hair loss was assessed by patients using a 100-point Visual Analog Scale (VAS) and by physicians using the 5-point Dean Scale at baseline, every 3-4 weeks during chemotherapy, and at least 1 month after completion of chemotherapy...
September 18, 2017: Breast Cancer Research and Treatment
https://www.readbyqxmd.com/read/28921169/effects-of-acute-salt-stress-on-modulation-of-gene-expression-in-a-malaysian-salt-tolerant-indigenous-rice-variety-bajong
#15
Brandon Pei Hui Yeo, Mrinal Bhave, Siaw San Hwang
The small genome size of rice relative to wheat and barley, together with its salt sensitivity, make it an ideal candidate for studies of salt stress response. Transcriptomics has emerged as a powerful technique to study salinity responses in many crop species. By identifying a large number of differentially expressed genes (DEGs) simultaneously after the stress induction, it can provide crucial insight into the immediate responses towards the stressor. In this study, a Malaysian salt-tolerant indigenous rice variety named Bajong and one commercial rice variety named MR219 were investigated for their performance in plant growth and ion accumulation properties after salt stress treatment...
September 18, 2017: Journal of Plant Research
https://www.readbyqxmd.com/read/28920826/hydrogenophaga-soli-sp-nov-isolated-from-rice-field-soil
#16
Dahye Yang, Seho Cha, Jiwon Choi, Taegun Seo
A novel Gram-stain-negative bacterial strain, designated strain S10T, was isolated from soil collected in a rice field in Goyang, South Korea. Cells of strain S10T were strictly aerobic, motile and rod-shaped. Colonies were round, convex, smooth and white. The strain grew optimally at 37 °C, pH 7.0 and 0 % (w/v) NaCl. Phylogenetic analysis of the 16S rRNA gene sequence of strain S10T revealed that the bacterium belongs to the family Comamonadaceae and is related to members of the genus Hydrogenophaga, with Hydrogenophaga caeni EMB71T being its closest relative (97...
September 18, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28920415/effects-of-pig-breed-on-nutrient-and-amino-acid-digestibility-in-diets-with-different-fiber-sources
#17
Jinbiao Zhao, Qiuyun Wang, Ling Liu, Yiqiang Chen, Aiming Jin, Guoliang Liu, Kaizhen Li, Defa Li, Changhua Lai
Objective: This experiment was conducted to determine the differences in the apparent ileal (AID) and total tract digestibility (ATTD) of nutrient and indispensable amino acid (IAA) in high-fiber diets with wheat middlings, rice bran or alfalfa meal fed to Duroc x Landrace x Yorkshire (DLY) and Duroc x Berkshire x Jiaxing (DBJ) growing pigs. Methods: Eighteen DLY and 18 DBJ pigs were randomly allotted to a 2 x 3 factorial arrangement involving 2 pig breeds and 3 high-fiber diets...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28919813/a-prospective-double-blinded-randomized-comparison-of-ultrasound-guided-femoral-nerve-block-with-lateral-femoral-cutaneous-nerve-block-versus-standard-anesthetic-management-for-pain-control-during-and-after-traumatic-femur-fracture-repair-in-the-pediatric-population
#18
Nicole M Elsey, Joseph D Tobias, Kevin E Klingele, Ralph J Beltran, Tarun Bhalla, David Martin, Giorgio Veneziano, Julie Rice, Dmitry Tumin
BACKGROUND: Traumatic injury of the femur resulting in femoral fracture may result in significant postoperative pain. As with other causes of acute pain, regional anesthesia may offer a benefit over conventional therapy with intravenous opioids. This study prospectively assesses the effects of femoral nerve blockade with a lateral femoral cutaneous nerve block (FN-LFCN) on intraoperative anesthetic requirements, postoperative pain scores, and opioid requirements. MATERIALS AND METHODS: Seventeen pediatric patients (age 2-18 years) undergoing surgical repair of a traumatic femur fracture fulfilled the study criteria and were randomly assigned to general anesthesia with either an FN-LFCN block (n = 10) or intravenous opioids (n = 7)...
2017: Journal of Pain Research
https://www.readbyqxmd.com/read/28918076/simultaneous-detection-of-wheat-dwarf-virus-northern-cereal-mosaic-virus-barley-yellow-striate-mosaic-virus-and-rice-black-streaked-dwarf-virus-in-wheat-by-multiplex-rt-pcr
#19
Peipei Zhang, Yan Liu, Wenwen Liu, Sebastien Massart, Xifeng Wang
Wheat dwarf virus (WDV), barley yellow striate mosaic virus (BYSMV), rice black-streaked dwarf virus (RBSDV) and northern cereal mosaic virus (NCMV) are four viruses infecting wheat and causing similar symptoms. In this paper, a multiplex reverse transcription polymerase chain reaction (m-RT-PCR) method has been developed for the simultaneous detection and discrimination of these viruses. The protocol uses specific primer set for each virus and produces four distinct fragments (273, 565, 783 and 1296bp), detecting the presence of RBSDV, BYSMV, WDV and NCMV, respectively...
September 13, 2017: Journal of Virological Methods
https://www.readbyqxmd.com/read/28918028/human-dmbt1-derived-cell-penetrating-peptides-for-intracellular-sirna-delivery
#20
Martina Tuttolomondo, Cinzia Casella, Pernille Lund Hansen, Ester Polo, Luciana M Herda, Kenneth A Dawson, Henrik J Ditzel, Jan Mollenhauer
Small interfering RNA (siRNA) is a promising molecule for gene therapy, but its therapeutic administration remains problematic. Among the recently proposed vectors, cell-penetrating peptides show great promise in in vivo trials for siRNA delivery. Human protein DMBT1 (deleted in malignant brain tumor 1) is a pattern recognition molecule that interacts with polyanions and recognizes and aggregates bacteria. Taking advantage of these properties, we investigated whether specific synthetic DMBT1-derived peptides could be used to formulate nanoparticles for siRNA administration...
September 15, 2017: Molecular Therapy. Nucleic Acids
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