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https://www.readbyqxmd.com/read/28735452/characterization-of-polymeric-substance-classes-in-cereal-based-beverages-using-asymmetrical-flow-field-flow-fractionation-with-a-multi-detection-system
#1
Georg Krebs, Thomas Becker, Martina Gastl
Cereal-based beverages contain a complex mixture of various polymeric macromolecules including polysaccharides, peptides, and polyphenols. The molar mass of polymers and their degradation products affect different technological and especially sensory parameters of beverages. Asymmetrical flow field-flow fractionation (AF4) coupled with multi-angle light scattering (MALS) and refractive index detection (dRI) or UV detection (UV) is a technique for structure and molar mass distribution analysis of macromolecules commonly used for pure compound solutions...
July 22, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28735321/betaine-improves-polymer-grade-d-lactic-acid-production-by-sporolactobacillus-inulinus-using-ammonia-as-green-neutralizer
#2
Guoping Lv, Chengchuan Che, Li Li, Shujing Xu, Wanyi Guan, Baohua Zhao, Jiansong Ju
The traditional CaCO3-based fermentation process generates huge amount of insoluble CaSO4 waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/L of D-lactic acid production and 0.89 g per g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by adding 100 mg/L betaine in the simple batch fermentation process. The addition of betaine was experimentally proven to protect cell at high concentration of ammonium ion, increase the D-lactate dehydrogenase specific activity and thus promote the production of D-lactic acid...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735312/effect-of-lyophilization-on-survivability-and-growth-kinetic-of-trichoderma-strains-preserved-on-various-agriculture-by-products
#3
Danuta Witkowska, Katarzyna Buska-Pisarek, Wojciech Łaba, Michał Piegza, Anna Kancelista
Growth of four Trichoderma strains were tested on lignocellulosic by-products in solid state fermentation (SSF). The strains were also analyzed for their survival rate and growth after lyophilization on these carriers. All applied monocomponent and bicomponent media were substrates for the production and preservation of Trichoderma biomass. However, the maximum number of colony forming units (CFU/g dm) was acquired on bicomponent media based on dried grass and beet pulp or grass with corn cobs, when compared to monocomponent media...
July 6, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735228/the-role-of-nitrogen-uptake-on-the-competition-ability-of-three-vineyard-saccharomyces-cerevisiae-strains
#4
Chiara Vendramini, Gemma Beltran, Chiara Nadai, Alessio Giacomini, Albert Mas, Viviana Corich
Three vineyard strains of Saccharomyces cerevisiae, P301.4, P304.4 and P254.12, were assayed in comparison with a commercial industrial strain, QA23. The aim was to understand if nitrogen availability could influence strain competition ability during must fermentation. Pairwise-strain fermentations and co-fermentations with the simultaneous presence of the four strains were performed in synthetic musts at two nitrogen levels: control nitrogen condition (CNC) that assured the suitable assimilable nitrogen amount required by the yeast strains to complete the fermentation and low nitrogen condition (LNC) where nitrogen is present at very low level...
July 13, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28734601/influence-of-saccharomyces-cerevisiae-fermentation-products-smartcare-in-milk-replacer-and-original-xpc-in-calf-starter-on-the-performance-and-health-of-preweaned-holstein-calves-challenged-with-salmonella-enterica-serotype-typhimurium
#5
T L Harris, Y Liang, K P Sharon, M D Sellers, I Yoon, M F Scott, J A Carroll, M A Ballou
This study was designed to investigate the effects of supplementing SmartCare (SC; Diamond V, Cedar Rapids, IA) in milk replacer and Original XPC (XPC; Diamond V) in calf starter on performance and health of preweaned calves following an oral challenge with Salmonella enterica. The study was performed in two 35-d periods with 30 Holstein bull calves (2 ± 1 d of age) per period. In each period, calves were blocked by location in the barn and randomly assigned to treatments that included control, base milk replacer and calf starter with no added Saccharomyces cerevisiae fermentation products; SC, milk replacer with 1 g of SC/calf per day and base calf starter; and SC+XPC, milk replacer with 1 g of SC/calf per day and calf starter with 0...
July 19, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28734365/automatic-feed-phase-identification-in-multivariate-bioprocess-profiles-by-sequential-binary-classification
#6
Ramin Nikzad-Langerodi, Edwin Lughofer, Susanne Saminger-Platz, Thomas Zahel, Patrick Sagmeister, Christoph Herwig
In this paper, we propose a new strategy for retrospective identification of feed phases from online sensor-data enriched feed profiles of an Escherichia Coli (E. coli) fed-batch fermentation process. In contrast to conventional (static), data-driven multi-class machine learning (ML), we exploit process knowledge in order to constrain our classification system yielding more parsimonious models compared to static ML approaches. In particular, we enforce unidirectionality on a set of binary, multivariate classifiers trained to discriminate between adjacent feed phases by linking the classifiers through a one-way switch...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734355/highly-sensitive-strategy-for-identification-of-trace-chemicals-in-complex-matrix-application-to-analysis-of-monacolin-analogues-in-monascus-fermented-rice-product
#7
Meng-Ning Li, Chao-Ran Li, Wen Gao, Ping Li, Hua Yang
Trace analysis of chemical analogues was always a hot topic and attracted the researchers' attentions. In this study, a database/multi-step filtering strategy was developed by using HPLC-Chip-MS system for comprehensive characterization of monacolin analogues in monascus-fermented rice product (MFRP). This strategy mainly included three steps, including chemical profile of MFRP by HPLC-Chip-MS, establishment of monacolin analogue database, multi-step filtering of monacolins based on modified mass defect filtering...
August 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28734048/non-anthocyanin-polyphenolic-transformation-by-native-yeast-and-bacteria-co-inoculation-strategy-during-vinification
#8
Apramita Devi, K M Archana, P K Bhavya, K A Anu Appaiah
BACKGROUND: Co-inoculation has been adapted by many wine-producing countries as it enhances the success of malolactic fermentation and reduces the fermentation cost as well as time. However, wine phenolics have been sparsely highlighted during co-inoculation even though polyphenols are important parameter affecting wine colour, astringency and aroma. Here, we investigate the impact of co-inoculation on non-anthocyanin polyphenol profile for two different grape varieties. RESULT: Co-inoculation of native yeast strain (AAV2) along with Oenococcus oeni was adapted for Cabernet Sauvignon and Shiraz wine...
July 22, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28733762/reconsidering-betaine-as-a-natural-anti-heat-stress-agent-in-poultry-industry-a-review
#9
REVIEW
Muhammad Saeed, Daryoush Babazadeh, Muhammad Naveed, Muhammad Asif Arain, Faiz Ul Hassan, Sun Chao
Betaine is found ubiquitously in plants, animals, microorganisms, and rich dietary sources including seafood, spinach, and wheat bran. The chief physiological role of betaine is to function as a methyl donor and an osmolyte. Betaine also acts as an osmolyte, to maintain the avian's cellular water and ion balance to improve the avian's capacity against heat stress via preventing dehydration and osmotic inactivation. It helps in maintaining the protective osmolytic activity, especially in heat-stressed birds...
July 21, 2017: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/28733718/antimicrobial-resistance-in-the-next-30%C3%A2-years-humankind-bugs-and-drugs-a-visionary-approach
#10
REVIEW
Matteo Bassetti, Garyphallia Poulakou, Etienne Ruppe, Emilio Bouza, Sebastian J Van Hal, Adrian Brink
PURPOSE: To describe the current standards of care and major recent advances with regard to antimicrobial resistance (AMR) and to give a prospective overview for the next 30 years in this field. METHODS: Review of medical literature and expert opinion were used in the development of this review. RESULTS: There is undoubtedly a large clinical and public health burden associated with AMR in ICU, but it is challenging to quantify the associated excess morbidity and mortality...
July 21, 2017: Intensive Care Medicine
https://www.readbyqxmd.com/read/28733671/short-chain-fatty-acids-and-inulin-but-not-guar-gum-prevent-diet-induced-obesity-and-insulin-resistance-through-differential-mechanisms-in-mice
#11
Karolin Weitkunat, Christin Stuhlmann, Anna Postel, Sandra Rumberger, Maria Fankhänel, Anni Woting, Klaus Jürgen Petzke, Sabrina Gohlke, Tim J Schulz, Michael Blaut, Susanne Klaus, Sara Schumann
The role of dietary fibre and short-chain fatty acids (SCFA) in obesity development is controversially discussed. Here, we investigated how various types of dietary fibre and different SCFA ratios affect metabolic syndrome-related disorders. Male mice (B6) were fed high-fat diets supplemented with dietary fibres (either cellulose, inulin or guar gum) or different Ac:Pr ratios (high acetate (HAc) or propionate (HPr)) for 30 weeks. Body-fat gain and insulin resistance were greatly reduced by inulin, but not by guar gum, and completely prevented by SCFA supplementation...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733629/improving-nitrogen-source-utilization-from-defatted-soybean-meal-for-nisin-production-by-enhancing-proteolytic-function-of-lactococcus-lactis-f44
#12
Jiaheng Liu, Jianjian Zhou, Lihong Wang, Zelin Ma, Guangrong Zhao, Zhiqiang Ge, Hongji Zhu, Jianjun Qiao
Nisin, one kind of natural antimicrobial peptide, is produced by certain Lactococcus lactis strains, which generally require expensive high-quality nitrogen sources due to limited ability of amino acids biosynthesis. Here we use defatted soybean meal (DSM) as sole nitrogen source to support L. lactis growth and nisin production. DSM medium composition and fermentation conditions were optimized using the methods of Plackett-Burman design and central composite design. The highest nisin production of 3879.58 IU/ml was obtained in DSM medium, which was 21...
July 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28733286/the-regulator-ldhr-and-the-d-lactate-dehydrogenase-ldha-of-burkholderia-multivorans-play-a-role-in-carbon-overflow-and-in-planktonic-cellular-aggregates-formation
#13
Inês N Silva, Marcelo J Ramires, Lisa A Azevedo, Ana R Guerreiro, Andreia C Tavares, Jörg D Becker, Leonilde M Moreira
LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co-transcribed with ldhA encoding a d-lactate dehydrogenase, and evaluate their implication in virulence traits like exopolysaccharide (EPS) synthesis and biofilm formation...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28733284/specific-signatures-of-the-gut-microbiota-and-increased-levels-of-butyrate-in-children-treated-with-fermented-cow-s-milk-containing-heat-killed-lactobacillus-paracasei-cba-l74
#14
Roberto Berni Canani, Francesca De Filippis, Rita Nocerino, Manolo Laiola, Lorella Paparo, Antonio Calignano, Carmen De Caro, Lorena Coretti, Lorenzo Chiariotti, Jack A Gilbert, Danilo Ercolini
We recently demonstrated that cow's milk fermented with the probiotic L.paracasei CBA L74 (FM-CBAL74) reduces the incidence of respiratory and gastrointestinal tract infections in young children attending school. This effect apparently derives from a complex regulation of non-immune and immune protective mechanisms. We investigated wheter FM-CBAL74 could regulate gut microbiota composition and butyrate production.We randomly selected 20 healthy children (12-48 months) from the previous randomized controlled trial, before (t0) and after 3 months (t3) of dietary treatment with FM-CBAL74 (FM), or placebo (PL)...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28731630/improved-pks-gene-expression-with-strong-endogenous-promoter-resulted-in-geldanamycin-yield-increase
#15
Xinran Wang, Xinjuan Ning, Qinqin Zhao, Qianjin Kang, Linquan Bai
The type I polyketide geldanamycin is a potent anti-tumor reagent. Its biosynthesis includes three steps: the biosynthesis of precursors, such as 3-amino-5-hydroxybenzoic acid (AHBA), the PKS chain extension, and the post-PKS modifications. According to the genomic and transcriptomic analysis, the PKS chain extension was deduced to be the rate-limiting step for geldanamycin production in Streptomyces hygroscopicus XM201. in order to improve the expression of PKS genes, a strong endogenous promoter 5063p was obtained based on the transcriptomic analysis and XylE enzymatic assay...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731563/improving-fructose-utilization-and-butanol-production-by-clostridium-acetobutylicum-via-extracellular-redox-potential-regulation-and-intracellular-metabolite-analysis
#16
Li-Jie Chen, You-Duo Wu, Chuang Xue, Feng-Wu Bai
Jerusalem artichoke (JA) can grow well in marginal lands with high biomass yield, and thus is a potential energy crop for biorefinery. The major biomass of JA is from tubers, which contain inulin that can be easily hydrolyzed into a mixture of fructose and glucose, but fructose utilization for producing butanol as an advanced biofuel is poor compared to glucose-based ABE fermentation by Clostridium acetobutylicum. In this article, the impact of extracellular redox potential (ORP) on the process was studied using a mixture of fructose and glucose to simulate the hydrolysate of JA tubers...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731533/improvement-of-d-lactic-acid-production-in-saccharomyces-cerevisiae-under-acidic-conditions-by-evolutionary-and-rational-metabolic-engineering
#17
Seung-Ho Baek, Eunice Y Kwon, Sang-Jeong Bae, Bo-Ram Cho, Seon-Young Kim, Ji-Sook Hahn
Microbial lactic acid (LA) production under acidic fermentation conditions is favorable to reduce the production cost, but circumventing LA toxicity is a major challenge. We generated D-LA-producing Saccharomyces cerevisiae strain JHY5610 by expressing D-lactate dehydrogenase gene (Lm. ldhA) from Leuconostoc mesenteroides, while deleting genes involved in ethanol production (ADH1, ADH2, ADH3, ADH4, and ADH5), glycerol production (GPD1 and GPD2), and degradation of D-LA (DLD1). Adaptive laboratory evolution of JHY5610 led to a strain JHY5710 having higher LA tolerance and D-LA-production capability...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731528/improving-succinate-productivity-by-engineering-a-cyanobacterial-co2-concentrating-system-ccm-in-escherichia-coli
#18
Mengyong Xiao, Xinna Zhu, Changhao Bi, Yanhe Ma, Xueli Zhang
Biologically fixation of CO2 has great potential as a significant carbon source for biosynthesis, which is also a major way to reduce CO2 accumulation in atmosphere. Phosphoenolpyruvate (PEP) carboxylation is the key step of anaerobic succinate production in Escherichia coli. In this reaction, one mole CO2 is assimilated with PEP to form oxaloacetate by PEP carboxykinase (PCK). The preferred substrate of PCK is CO2 , which is very limited in cytoplasm. In this study, the carbon concentration mechanism (CCM) of cyanobacteria was introduced into E...
July 21, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28731503/impact-of-soybean-aging-conditions-on-tofu-sensory-characteristics-and-acceptance
#19
Neide Kiyoko Kondo Kamizake, Lilian Catarini Pereira Silva, Sandra Helena Prudencio
BACKGROUND: Tofu from aged soybeans has poor quality, mainly texture. Texture defects related in literature are contradictory. No study has approached all sensory properties simultaneously. The objective of this study was to evaluate the effect of soybean aging conditions (natural: ambient temperature and RH, 18 months, and accelerated: 30 °C, 84% RH, 6 months) on all the sensory characteristics and acceptance of tofu. Texture and color were also evaluated by instrumental method. Control condition was -20 °C, 47% RH...
July 21, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28731144/dietary-fiber-in-irritable-bowel-syndrome-review
#20
Magdy El-Salhy, Synne Otterasen Ystad, Tarek Mazzawi, Doris Gundersen
Irritable bowel syndrome (IBS) is a common chronic gastrointestinal disorder. It is widely believed that IBS is caused by a deficient intake of dietary fiber, and most physicians recommend that patients with IBS increase their intake of dietary fiber in order to relieve their symptoms. However, different types of dietary fiber exhibit marked differences in physical and chemical properties, and the associated health benefits are specific for each fiber type. Short-chain soluble and highly fermentable dietary fiber, such as oligosaccharides results in rapid gas production that can cause abdominal pain/discomfort, abdominal bloating/distension and flatulence in patients with IBS...
July 19, 2017: International Journal of Molecular Medicine
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