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https://www.readbyqxmd.com/read/28324650/genome-engineering-of-virulent-lactococcal-phages-using-crispr-cas9
#1
Marie-Laurence Lemay, Denise M Tremblay, Sylvain Moineau
Phages are biological entities found in every ecosystem. Although much has been learned about them in past decades, significant knowledge gaps remain. Manipulating virulent phage genomes is challenging. To date, no efficient gene-editing tools exist for engineering virulent lactococcal phages. Lactococcus lactis is a bacterium extensively used as a starter culture in various milk fermentation processes and its phage sensitivity poses a constant risk to the cheese industry. The lactococcal phage p2 is one of the best-studied models for these virulent phages...
March 21, 2017: ACS Synthetic Biology
https://www.readbyqxmd.com/read/28324615/construction-of-low-ethanol-wine-yeasts-through-partial-deletion-of-the-saccharomyces-cerevisiae-pdc2-gene
#2
Raúl Andrés Cuello, Karina Johana Flores Montero, Laura Analía Mercado, Mariana Combina, Iván Francisco Ciklic
We propose an alternative GMO based strategy to obtain Saccharomyces cerevisiae mutant strains with a slight reduction in their ability to produce ethanol, but with a moderate impact on the yeast metabolism. Through homologous recombination, two truncated Pdc2p proteins Pdc2pΔ344 and Pdc2pΔ519 were obtained and transformed into haploid and diploid lab yeast strains. In the pdc2Δ344 mutants the DNA-binding and transactivation site of the protein remain intact, whereas in pdc2Δ519 only the DNA-binding site is conserved...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28319775/dilute-alkali-pretreatment-of-softwood-pine-a-biorefinery-approach
#3
Ali Safari, Keikhosro Karimi, Marzieh Shafiei
Dilute alkali pretreatment was performed on softwood pine to maximize ethanol and biogas production via a biorefinery approach. Alkali pretreatments were performed with 0-2% w/v NaOH at 100-180°C for 1-5h. The liquid fraction of the pretreated substrates was subjected to anaerobic digestion. The solid fraction of the pretreatment was used for separate enzymatic hydrolysis and fermentation. High ethanol yields of 76.9‒78.0% were achieved by pretreatment with 2% (w/v) NaOH at 180°C. The highest biogas yield of 244mL/g volatile solid (at 25°C, 1bar) was achieved by the pretreatment with 1% (w/v) NaOH at 180°C...
March 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319764/electrofluid-hydrolysis-enhances-the-production-of-fermentable-sugars-from-corncob-via-in-reverse-phase-induced-voltage
#4
Fengfeng Wu, Yamei Jin, Dandan Li, Yuyi Zhou, Lunan Guo, Mengyue Zhang, Xueming Xu, Na Yang
To improve the economic value of lignocellulosic biomasses, an innovative electrofluidic technology has been applied to the efficient hydrolysis of corncob. The system combines fluidic reactors and induced voltages via magnetoelectric coupling effect. The excitation voltage had a positive impact on reducing sugar content (RSC). But, the increase of voltage frequency at 400-700Hz caused a slight decline of the RSC. Higher temperature limits the electrical effect on the hydrolysis at 70-80°C. The energy efficiency increased under the addition of metallic ions and series of in-phase induced voltage to promote hydrolysis...
March 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28319022/recovery-of-lignin-peroxidase-from-submerged-liquid-fermentation-of-amauroderma-rugosum-blume-t-nees-torrend-using-polyethylene-glycol-salt-aqueous-two-phase-system
#5
Wan Yng Linda Jong, Pau Loke Show, Tau Chuan Ling, Yee Shin Tan
Amauroderma rugosum is a wild mushroom species widely distributed in tropics and is classified under the class of Basidiomycetes. Basidiomycetes are well-known for their abilities of producing lignocellulolytic enzymes such as lignin peroxidase (LiP), laccase (Lac) and manganese peroxidase (MnP). Different factors such as nutrient sources, incubation period and agitation affect the production of lignocellulolytic enzymes. The A. rugosum produced LiP in the medium supplemented with potato dextrose broth (PDB), 0...
March 16, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28318581/characterization-of-the-chemical-structures-and-physical-properties-of-exopolysaccharides-produced-by-various-streptococcus-thermophilus-strains
#6
U Pachekrepapol, J A Lucey, Y Gong, R Naran, P Azadi
Exopolysaccharides (EPS) produced by some lactic acid bacteria are often used by the dairy industry to improve the rheological and physical properties of yogurt, but the relationship between their structure and functional effect is still unclear. The EPS from different species, or different strains from the same species, may differ in terms of molar mass, repeating unit structure, and EPS yield during fermentation of milk. This study aimed to characterize the detailed properties of EPS produced from 7 strains of Streptococcus thermophilus, which is one of the key cultures used for yogurt manufacture...
March 16, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28318400/new-frontiers-in-fibers-innovative-and-emerging-research-on-the-gut-microbiome-and-bone-health
#7
Taylor C Wallace, Massimo Marzorati, Lisa Spence, Connie M Weaver, Patricia S Williamson
The complex interactions between the diet, gut microbiome, and host characteristics that provide a functional benefit to the host are an area of great interest and current exploration in the nutrition and health science community. New technologies are available to assess mechanisms that may explain these functional benefits to the host. One emerging functional benefit from changes in the gut microbiome is increased calcium absorption, increased calcium retention, and improved indices of bone health. Prebiotic fibers enhance microbial fermentation in the gut, providing an ecological advantage to specific nonpathogenic bacteria that have the ability to modify an individual's metabolic potential...
March 20, 2017: Journal of the American College of Nutrition
https://www.readbyqxmd.com/read/28318272/investigations-on-the-aroma-of-cocoa-pulp-theobroma-cocoa-l-and-its-influence-on-the-odor-of-fermented-cocoa-beans
#8
Irene Chetschik, Markus Kneubühl, Karin Chatelain, Ansgar Schlüter, Konrad Bernath, Tilo Hühn
The odor active constituents of cocoa pulp have been analyzed by aroma extract dilution analysis (AEDA) for the first time. Pulps of three different cocoa varieties have been investigated. The variety CCN51 showed low flavor intensities in terms of FD-factors, in comparison to the varieties FSV41 and UF564, for which floral and fruity notes were detected in higher intensities. In order to gain first insights on molecular level how the cocoa pulp odorants impacted the odor quality of cocoa beans during fermentation, quantitative measurements of selected aroma compounds were conducted in pulp and bean at different time points of the fermentation...
March 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28317770/characterization-enhancement-and-modelling-of-mannosylerythritol-lipid-production-by-fungal-endophyte-ceriporia-lacerate-chzju
#9
Yongwu Niu, Linlin Fan, Di Gu, Jianan Wu, Qihe Chen
The glycolipid biosurfactants mannosylerythritol lipids (MELs) attract great attention for their biodegradability, super emulsifying properties and versatile bioactivities. In this study, the MEL deriving from Ceriporia lacerate CHZJU was identified as MEL-A, and its critical micelle concentration and emulsifying activities were assessed. To examine the production of MELs from Ceriporia lacerate, a Plackett-Burman design and response surface methodology were used to optimize the culture nutrients. The optimal medium contains 1g/L yeast extract, 1...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317762/evaluation-of-aroma-enhancement-for-ecolly-dry-white-wines-by-mixed-inoculation-of-selected-rhodotorula-mucilaginosa-and-saccharomyces-cerevisiae
#10
Xing-Chen Wang, Ai-Hua Li, Marta Dizy, Niamat Ullah, Wei-Xuan Sun, Yong-Sheng Tao
To improve the aroma profile of Ecolly dry white wine, the simultaneous and sequential inoculations of selected Rhodotorula mucilaginosa and Saccharomyces cerevisiae were performed in wine making of this work. The two yeasts were mixed in various ratios for making the mixed inoculum. The amount of volatiles and aroma characteristics were determined the following year. Mixed fermentation improved both the varietal and fermentative aroma compound composition, especially that of (Z)-3-hexene-1-ol, nerol oxide, certain acetates and ethyls group compounds...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317753/bioactive-amines-and-phenolic-compounds-in-cocoa-beans-are-affected-by-fermentation
#11
Brenda de Nazaré do Carmo Brito, Renan Campos Chisté, Rosinelson da Silva Pena, Maria Beatriz Abreu Gloria, Alessandra Santos Lopes
Cocoa is the target of increased scientific research as it is one of the richest source of bioactive compounds. The formation of bioactive amines and their changes in cocoa beans during seven days of traditional fermentation was investigated for the first time. In addition, total phenolic compounds, anthocyanins contents and the scavenging capacity against ABTS radical were determined to monitor the fermentation process. Only two biogenic amines (tryptamine and tyramine) and two polyamines (spermidine and spermine) were detected in cocoa beans during fermentation...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317725/simultaneous-quantification-of-twenty-amadori-products-in-soy-sauce-using-liquid-chromatography-tandem-mass-spectrometry
#12
Hiroshi Katayama, Yuki Tatemichi, Ayako Nakajima
A liquid chromatography-tandem mass spectrometry method using a pentafluorophenylpropyl-bonded silica column was developed to simultaneously quantify twenty Amadori products (APs), including N-(1-Deoxy-d-fructosyl-1-yl)-l-isoleucine (Fru-Ile) and N-(1-Deoxy-d-fructosyl-1-yl)-l-leucine (Fru-Leu), in soy sauce, without the need for an ion-pairing reagent or sample derivatization. The method was applied to six types of soy sauce, to determine the total AP levels and the levels of individual APs. The level of total APs widely varied between the eight samples, from 358mg/L to 24347mg/L...
August 1, 2017: Food Chemistry
https://www.readbyqxmd.com/read/28317285/changes-in-the-relative-population-size-of-selected-ruminal-bacteria-following-an-induced-episode-of-acidosis-in-beef-heifers-receiving-viable-and-non-viable-active-dried-yeast
#13
R Mohammed, D Vyas, W Z Yang, K A Beauchemin
AIMS: To characterize the changes in the relative population size (RPS) of select ruminal bacteria and rumen fermentation variables in beef heifers supplemented with a strain of Saccharomyces cerevisiae as viable active dried (ADY) or killed dried (KDY) yeast following an induced episode of ruminal acidosis. METHODS AND RESULTS: Six ruminally-cannulated beef heifers fed a diet consisting of 50% forage and 50% grain (dry matter basis) were used in a replicated 3 × 3 Latin square design with three 28 d periods...
March 20, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28315963/enhancement-of-2-3-butanediol-production-from-jerusalem-artichoke-tuber-extract-by-a-recombinant-bacillus-sp-strain-brc1-with-increased-inulinase-activity
#14
Jang Min Park, Baek-Rock Oh, In Yeong Kang, Sun-Yeon Heo, Jeong-Woo Seo, Seung-Moon Park, Won-Kyung Hong, Chul Ho Kim
A Bacillus sp. strain named BRC1 is capable of producing 2,3-butanediol (2,3-BD) using hydrolysates of the Jerusalem artichoke tuber (JAT), a rich source of the fructose polymer inulin. To enhance 2,3-BD production, we undertook an extensive analysis of the Bacillus sp. BRC1 genome, identifying a putative gene (sacC) encoding a fructan hydrolysis enzyme and characterizing the activity of the resulting recombinant protein expressed in and purified from Escherichia coli. Introduction of the sacC gene into Bacillus sp...
March 18, 2017: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28315928/boosting-the-growth-of-the-probiotic-strain-lactobacillus-paracasei-ssp-paracasei-f19
#15
Desideria Brignone, Pia Radmann, Jürgen Behr, Rudi F Vogel
Single so-called booster substances were added to the fermentation medium of the probiotic strain Lactobacillus (L.) paracasei ssp. paracasei F19 to enhance its growth. A wide screening was carried out in microtiter plates and a statistical analysis of the growth parameters was performed. CFU counts were used to correlate the increase in OD590nm with the increase in viable cell number. Sodium ascorbate, sodium pyruvate, manganese sulfate and cysteine had a remarkable boosting effect on the growth of L. paracasei F19...
March 18, 2017: Archives of Microbiology
https://www.readbyqxmd.com/read/28315821/1-3-propanediol-production-by-a-newly-isolated-strain-clostridium-perfringens-gyl
#16
Yali Guo, Lu Dai, Bo Xin, Fei Tao, Hongzhi Tang, Yaling Shen, Ping Xu
1,3-Propanediol (1,3-PD), a valuable bulk industrial material, has attracted increasing interest in recent years. A novel strain GYL isolated from soil samples could efficiently convert glycerol to 1,3-PD anaerobically. The physiological and biochemical characteristics of strain GYL were determined, indicating that strain GYL is a member of Clostridium perfringens with the neighbor-joining method of 16S rRNA gene sequences. The fermentation properties of strain GYL were also investigated systematically, which showed that the strain has a fast growth speed and high tolerance to 200g/L glycerol...
February 28, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28315603/dynamic-membrane-assisted-fermentation-of-food-wastes-for-enhancing-lactic-acid-production
#17
Jialing Tang, Xiaochang C Wang, Yisong Hu, Huu Hao Ngo, Yuyou Li
A dynamic membrane (DM) module was inserted into a fermentation reactor to separate soluble products from the fermented mixture to increase lactic acid (LA) production from food wastes under acidogenic conditions (uncontrolled pH, pH 4 and 5). With a high total suspended solid content (20-40g/L) in the fermenter, a stable DM could be maintained through regular backwashing. By effectively intercepting suspended solids and lactic acid bacteria (LAB), the fermenter was able to increase microbial activity and largely promote LA yield...
March 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28315440/optimized-production-and-characterization-of-a-detergent-stable-protease-from-lysinibacillus-fusiformis-c250r
#18
REVIEW
Sondes Mechri, Mouna Kriaa, Mouna Ben Elhoul Berrouina, Maroua Omrane Benmrad, Nadia Zaraî Jaouadi, Hatem Rekik, Khelifa Bouacem, Amel Bouanane-Darenfed, Alif Chebbi, Sami Sayadi, Mohamed Chamkha, Samir Bejar, Bassem Jaouadi
In this study, we aimed to optimize the cultural and nutritional conditions for protease production by Lysinibacillus fusiformis strain C250R in submerged fermentation process using statistical methodology. The most significant factors (gruel, wheat bran, yeast extract, and FeSO4) were identified by Plackett-Burman design. Response surface methodology (RSM) was used to determine the optimum levels of the screened factors and their interaction. Under the optimized conditions, protease yield 3100U/mL was 4.5 folds higher than those obtained by the use of the initial conditions (680U/ml)...
March 14, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28315397/efficient-extracellular-expression-of-transpeptidase-sortase-a-in-pichia-pastoris
#19
Xinrui Zhao, Haofei Hong, Zhimeng Wu
In order to achieve efficient extracellular expression of Sortase A (SrtA), various strategies in Pichia pastoris system were applied in this study. Among different constructed recombinant strains, the SMD1168 strain integrated 5.7 copies of srtA gene under control of AOX1 promoter was proved to be the best strain for the extracellular SrtA expression. After the optimization of fermentation conditions (induction 72 h at 28 °C, initial pH 6.0, supplemented with 1.5% methanol), the highest yield and activity of extracellular SrtA reached 97...
March 15, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/28314829/phosphorylation-of-wrinkled1-by-kin10-results-in-its-proteasomal-degradation-providing-a-link-between-energy-homeostasis-and-lipid-biosynthesis
#20
Zhiyang Zhai, Hui Liu, John Shanklin
WRINKLED1 (WRI1), a member of the APETALA2 (AP2) class of transcription factors, positively regulates glycolysis and lipid biosynthesis in Arabidopsis thaliana. Here we identify mechanistic links between KIN10, the major SUCROSE NON-FERMENTATION-1 (SNF1)-RELATED KINASE 1 (SnRK1) involved in sugar/energy homeostasis and the posttranslational regulation of WRI1. Transient expression of WRI1 with OLEOSIN1 (OLE1) in Nicotiana benthamiana stimulates triacylglycerol (TAG) accumulation, but their coexpression with KIN10 abrogates this effect by inducing proteasomal degradation of WRI1...
March 17, 2017: Plant Cell
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