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https://www.readbyqxmd.com/read/29342350/real-time-monitoring-of-chemical-changes-in-three-kinds-of-fermented-milk-products-during-fermentation-using-quantitative-difference-nmr-spectroscopy
#1
Yi Lu, Hiroto Ishikawa, Yeondae Kwon, Fangyu Hu, Takuya Miyakawa, Masaru Tanokura
Fermented milk products are rising in popularity throughout the world because of their health benefits, including improving digestion, normalizing the function of the immune system, and aiding in weight management. This study applies an in situ quantitative NMR method to monitor chemical changes in three kinds of fermented milk products, Bulgarian yogurt, Caspian Sea yogurt and kefir, during fermentation. As a result, the concentration changes in nine organic compounds, α/β-lactose, α/β-galactose, lactic acid, citrate, ethanol, lecithin, and creatine, were monitored in real time...
January 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29342098/mechano-enzymatic-deconstruction-with-a-new-enzymatic-cocktail-to-enhance-enzymatic-hydrolysis-and-bioethanol-fermentation-of-two-macroalgae-species
#2
Sameh Amamou, Cecilia Sambusiti, Florian Monlau, Eric Dubreucq, Abdellatif Barakat
The aim of this study was to explore the efficiency of a mechano-enzymatic deconstruction of two macroalgae species for sugars and bioethanol production, by using a new enzymatic cocktail (Haliatase) and two types of milling modes (vibro-ball: VBM and centrifugal milling: CM). By increasing the enzymatic concentration from 3.4 to 30 g/L, the total sugars released after 72 h of hydrolysis increased (from 6.7 to 13.1 g/100 g TS and from 7.95 to 10.8 g/100 g TS for the green algae U. lactuca and the red algae G...
January 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29341954/carbapenemase-production-of-clinical-isolates-acinetobacter-baumannii-and-pseudomonas-aeruginosa-from-a-bulgarian-university-hospital
#3
Atanaska P Petrova, Irina D Stanimirova, Ivan N Ivanov, Michael M Petrov, Tsonka M Miteva-Katrandzhieva, Vasil I Grivnev, Velichka S Kardjeva, Todor V Kantardzhiev, Mariana A Murdjeva
BACKGROUND: Production of Bla OXA-23, OXA-24, OXA-58 and hyperexpression of OXA-51 due to ISAba1 insertion sequence are the leading causes of carbapenem resistance in Acinetobacter baumannii. The loss of OprD transmembrane protein and the overexpression of some effl ux pumps are considered to be the main factors for carbapenem resistance in Pseudomonas aeruginosa whereas metallo-enzymes' production has a secondary role. AIM: Тo examine the carbapenem resistance due to carbapenemase production among clinically signifi cant Gram-negative non-fermenters from St George University hospital, Plovdiv: A...
December 20, 2017: Folia Medica
https://www.readbyqxmd.com/read/29340150/xylose-absorption-fermentation-and-post-absorptive-metabolism-in-the-pig
#4
REVIEW
Nichole F Huntley, John F Patience
Xylose, as β-1,4-linked xylan, makes up much of the hemicellulose in cell walls of cereal carbohydrates fed to pigs. As inclusion of fibrous ingredients in swine diets continues to increase, supplementation of carbohydrases, such as xylanase, is of interest. However, much progress is warranted to achieve consistent enzyme efficacy, including an improved understanding of the utilization and energetic contribution of xylanase hydrolysis product (i.e. xylooligosaccharides or monomeric xylose). This review examines reports on xylose absorption and metabolism in the pig and identifies gaps in this knowledge that are essential to understanding the value of carbohydrase hydrolysis products in the nutrition of the pig...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29339973/one-step-production-of-c6-c8-carboxylates-by-mixed-culture-solely-grown-on-co
#5
Pinjing He, Wenhao Han, Liming Shao, Fan Lü
Background: This study aimed at producing C6-C8 medium-chain carboxylates (MCCAs) directly from gaseous CO using mixed culture. The yield and C2-C8 product composition were investigated when CO was continuously fed with gradually increasing partial pressure. Results: The maximal concentrations of n-caproate, n-heptylate, and n-caprylate were 1.892, 1.635, and 1.033 mmol L-1, which were achieved at the maximal production rates of 0.276, 0.442, and 0.112 mmol L-1 day-1, respectively...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/29339339/proteomics-and-genetic-analyses-reveal-the-effects-of-arsenite-oxidation-on-metabolic-pathways-and-the-roles-of-aior-in-agrobacterium-tumefaciens-gw4
#6
Kaixiang Shi, Qian Wang, Xia Fan, Gejiao Wang
A heterotrophic arsenite [As(III)]-oxidizing bacterium Agrobacterium tumefaciens GW4 isolated from As(III)-rich groundwater sediment showed high As(III) resistance and could oxidize As(III) to As(V). The As(III) oxidation could generate energy and enhance growth, and AioR was the regulator for As(III) oxidase. To determine the related metabolic pathways mediated by As(III) oxidation and whether AioR regulated other cellular responses to As(III), isobaric tags for relative and absolute quantitation (iTRAQ) was performed in four treatments, GW4 (+AsIII)/GW4 (-AsIII), GW4-ΔaioR (+AsIII)/GW4-ΔaioR (-AsIII), GW4-ΔaioR (-AsIII)/GW4 (-AsIII) and GW4-ΔaioR (+AsIII)/GW4 (+AsIII)...
January 12, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29339235/a-highly-efficient-two-stage-cultivation-strategy-for-lutein-production-using-heterotrophic-culture-of-chlorella-sorokiniana-mb-1-m12
#7
Chun-Yen Chen, I-Chia Lu, Dillirani Nagarajan, Chien-Hsiang Chang, I-Son Ng, Duu-Jong Lee, Jo-Shu Chang
A heterotrophic mutant of Chlorella sorokiniana MB-1-M12 was evaluated for its ability to produce lutein using organic carbon and nitrogen sources and without light irradiation. In batch fermentation, the maximal lutein content (3.67 mg lutein/g biomass) and productivity (2.84 mg/L/d) could be obtained when cultivated in BG-11 medium with 7.5 g/L glucose, 0.75 g/L urea, pH 7.5 and a C/N ratio of 10. A novel two-stage cultivation strategy that integrates fed-batch and semi-batch operations was applied to enhance the lutein production performance...
January 6, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29339233/ftir-as-an-easy-and-fast-analytical-approach-to-follow-up-microbial-growth-during-fungal-pretreatment-of-poplar-wood-with-phanerochaete-chrysosporium
#8
I Cornet, N Wittner, G Tofani, S Tavernier
Since the determination of the fermentation kinetics is one of the main challenges in solid state fermentation, the quantitative measurement of biomass growth during microbial pretreatment by FTIR spectroscopy in Attenuated Total Reflectance mode was evaluated. Peaks at wave numbers of 1651 cm-1 and 1593 cm-1 showed to be affected during pretreatment of poplar wood particles by Phanerochaete chrysosporium MUCL 19343. Samples with different microbial biomass fractions were obtained from two different experiments, i...
January 12, 2018: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/29339003/direct-recovery-of-bacillus-subtilis-xylanase-from-fermentation-broth-with-an-alcohol-salt-aqueous-biphasic-system
#9
Hui Suan Ng, Cindy Xin Yi Chai, Yin Hui Chow, Wai Leng Carmen Loh, Hip Seng Yim, Joo Shun Tan, John Chi-Wei Lan
Xylanase enzyme degrades linear polysaccharide β-1,4 xylan and the hemicellulose of the plant cell wall. There is a growing demand in finding a cost-effective alternative for industrial scale production of xylanase with high purity for pharmaceutical applications. In this study, an alcohol/salt aqueous biphasic system (ABS) was adopted to recover xylanase from the Bacillus subtilis fermentation broth. The effects of several ABS parameters such as types and concentrations of alcohols and salts (i.e., sulphate, phosphate, and citrate), amount of crude loading and pH of the system on the recovery of xylanase were investigated...
January 12, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29338435/three-new-diphenyl-ether-derivatives-from-the-fermentation-products-of-an-endophytic-fungus-phomopsis-fukushii
#10
Yu-Hong Gao, Rui Zheng, Jing Li, Wei-Song Kong, Xin Liu, Lin Ye, Qi-Li Mi, Wei-Song Kong, Min Zhou, Guang-Yu Yang, Qiu-Fen Hu, Gang Du, Hai-Ying Yang, Xue-Mei Li
Three new diphenyl ethers (1-3), together with four known isopentylated diphenyl ethers derivatives (4-7), were isolated from the fermentation products of an endophytic fungus Phomopsis fukushii. Their structures were elucidated by spectroscopic methods, including extensive 1D and 2D NMR techniques. Compounds 1-3 were evaluated for their anti-methicillin-resistant Staphylococcus aureus (anti-MRSA) activity. The results revealed that compounds 1 and 2 showed strong inhibitions with inhibition zone diameters (IZD) of 20...
January 17, 2018: Journal of Asian Natural Products Research
https://www.readbyqxmd.com/read/29338242/in-situ-biocatalytic-production-of-trehalose-with-auto-induction-expression-of-trehalose-synthase
#11
Xincheng Yan, Liying Zhu, Yadong Yu, Qing Xu, He Huang, Ling Jiang
We developed an in situ biocatalytic process that couples auto-induction expression of trehalose synthase (TreS) and whole-cell catalysis for trehalose production. With lactose as the auto-inducer, the activity of recombinant TreS in recombinant Escherichia coli was optimized through a visualization method, which resulted in a maximum value of 12033 ± 730 U/mL in pH-stat fed-batch fermentation mode. Meanwhile, the permeability of the auto-induced E. coli increased significantly, which makes it possible to be directly used as a whole-cell biocatalyst for trehalose production, whereby the by-product glucose can also act as an extra carbon source...
January 17, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29337626/comparative-analysis-of-pasteurella-multocida-isolates-from-acute-and-chronic-fowl-cholera-cases-in-hungary-during-the-period-2005-through-2010
#12
Boglárka Sellyei, Ákos Thuma, Dmitriy Volokhov, Zsuzsanna Varga
Fowl cholera (FC) is a highly contagious and economically important disease of poultry worldwide. This study was performed on 218 Pasteurella multocida isolates collected from separated breeding farms or backyards with acute and chronic FC cases in multiple localities across Hungary during the period 2005-2010. All isolates were characterized by a broad range of biochemical, serological, and molecular methods, as well as their antibiotic susceptibility to aminoglycosides (A), macrolides (M), penicillins (P), quinolones (Q), cephalosporins, sulphonamides (S), and tetracyclines (T) was determined...
December 2017: Avian Diseases
https://www.readbyqxmd.com/read/29337411/the-fourth-wave-of-biocatalysis-emerges-the-13%C3%A2-th-international-symposium-on-biocatalysis-and-biotransformations
#13
EDITORIAL
László Poppe, Beáta G Vértessy
Ride the wave! Biocatalysis uses nature's catalysts, enzymes and whole cell systems, for synthetic purposes. In a biotransformation, the biocatalyst transforms a well-defined substrate to the desired product, in contrast to the fermentation process, which produces the desired product from a complex mixture of nutrients. Biocatalysis has reached an industrially established level through several waves of technological evolution; participants of the BioTrans 2017 conference in Budapest could witness the newest wave of this technology...
January 16, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29337351/selection-of-yeast-strains-for-tequila-fermentation-based-on-growth-dynamics-in-combined-fructose-and-ethanol-media
#14
J A Aldrete-Tapia, D E Miranda-Castilleja, S M Arvizu-Medrano, M Hernández-Iturriaga
The high concentration of fructose in agave juice has been associated with reduced ethanol tolerance of commercial yeasts used for tequila production and low fermentation yields. The selection of autochthonous strains, which are better adapted to agave juice, could improve the process. In this study, a 2-step selection process of yeasts isolated from spontaneous fermentations for tequila production was carried out based on analysis of the growth dynamics in combined conditions of high fructose and ethanol. First, yeast isolates (605) were screened to identify strains tolerant to high fructose (20%) and to ethanol (10%), yielding 89 isolates able to grow in both conditions...
January 16, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29337072/valorization-of-brewer-s-spent-grain-to-prebiotic-oligosaccharide-production-xylanase-catalyzed-hydrolysis-in-vitro-evaluation-with-probiotic-strains-and-in-a-batch-human-fecal-fermentation-model
#15
Mursalin Sajib, Peter Falck, Roya R R Sardari, Sindhu Mathew, Carl Grey, Eva Nordberg Karlsson, Patrick Adlercreutz
Brewer's spent grain (BSG) accounts for around 85% of the solid by-products from beer production. BSG was first extracted to obtain water-soluble arabinoxylan (AX). Using subsequent alkali extraction (0.5 M KOH) it was possible to dissolve additional AX. In total, about 57% of the AX in BSG was extracted with the purity of 45-55%. After comparison of nine xylanases, Pentopan mono BG, a GH11 enzyme, was selected for hydrolysis of the extracts to oligosaccharides with minimal formation of monosaccharides. Growth of Bifidobacterium adolescentis (ATCC 15703) was promoted by the enzymatic hydrolysis to arabinoxylooligosaccharides, while Lactobacillus brevis (DSMZ 1264) utilized only unsubstituted xylooligosaccharides...
January 11, 2018: Journal of Biotechnology
https://www.readbyqxmd.com/read/29337032/biohydrogen-fermentation-of-galactose-at-various-substrate-concentrations-in-an-immobilized-system-and-its-microbial-correspondence
#16
Periyasamy Sivagurunathan, Arivalagan Pugazhendhi, Gopalakrishnan Kumar, Jong-Hun Park, Sang-Hyoun Kim
The effects of substrate concentration on fermentative hydrogen production from galactose at a fixed hydraulic retention time of 12 h were investigated in an immobilized continuously stirred tank reactor. Peak hydrogen production rate and hydrogen yield of 9.57 L/L-d and 1.10 mol/mol galactoseadded, respectively, were obtained at a feed substrate concentration of 30 g/L and an organic loading rate of 60 L/L-d. Quantitative polymerase chain reaction analysis showed that the variations in the performance resulted primarily from metabolic alterations within the metabolism of the established microbial community rather than modifications in the population...
January 11, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29336590/modern-perspectives-on-the-health-benefits-of-kefir-in-next-generation-sequencing-era-improvement-of-the-host-gut-microbiota
#17
Dong-Hyeon Kim, Dana Jeong, Hyunsook Kim, Kun-Ho Seo
Kefir is a natural complex fermented milk product containing more than 50 species of probiotic bacteria and yeast, and has been demonstrated to have multiple properties conferring health benefits, including antiobesity, anti-hepatic steatosis, antioxidative, antiallergenic, antitumor, anti-inflammatory, cholesterol-lowering, constipation-alleviating, and antimicrobial properties. To better understand the underlying mechanisms of these benefits, we here review research on the effect of kefir (and kefir microorganisms) consumption to modulate the host gut microbiota...
January 16, 2018: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29336182/the-effects-of-fermented-milk-containing-lactococcus-lactis-subsp-cremoris-fc-on-defaecation-in-healthy-young-japanese-women-a-double-blind-placebo-controlled-study
#18
Kaoru Ozaki, Toshinari Maruo, Hideki Kosaka, Mari Mori, Hideki Mori, Yukio Yamori, Toshiya Toda
The objective of this double-blind, placebo-controlled study was to elucidate the effects of fermented milk containing Lactococcus lactis subsp. cremoris FC (FC) on defaecation in healthy young women. We included 31 women (18-31 years old) who were randomly selected into two groups. Subjects in the test group consumed fermented milk containing FC, while subjects in the placebo group consumed non-fermented gelled milk. In the test group, defaecation frequency (both in days and times per week) and stool volume significantly increased during the consumption of fermented milk containing FC compared with before consumption...
January 16, 2018: International Journal of Food Sciences and Nutrition
https://www.readbyqxmd.com/read/29336079/the-low-fodmap-diet-in-the-management-of-irritable-bowel-syndrome-an-evidence-based-review-of-fodmap-restriction-reintroduction-and-personalisation-in-clinical-practice
#19
REVIEW
K Whelan, L D Martin, H M Staudacher, M C E Lomer
Dietary restriction of fermentable oligosaccharides, disaccharides, monosaccharides and polyols (FODMAPs) is effective in the management of functional gastrointestinal symptoms that occur in irritable bowel syndrome (IBS). Numerous reviews have been published regarding the evidence for their restriction in the low FODMAP diet; however, few reviews discuss the implementation of the low FODMAP diet in practice. The aim of this review is to provide practical guidance on patient assessment and the implementation and monitoring of the low FODMAP diet...
January 15, 2018: Journal of Human Nutrition and Dietetics: the Official Journal of the British Dietetic Association
https://www.readbyqxmd.com/read/29335876/hydrogen-metabolic-patterns-driven-by-clostridium-streptococcus-community-shifts-in-a-continuous-stirred-tank-reactor
#20
Rodolfo Palomo-Briones, Eric Trably, Nguyen Esmeralda López-Lozano, Lourdes B Celis, Hugo Oscar Méndez-Acosta, Nicolas Bernet, Elías Razo-Flores
The hydrogen (H2) production efficiency in dark fermentation systems is strongly dependent on the occurrence of metabolic pathways derived from the selection of microbial species that either consume molecular H2 or outcompete hydrogenogenic bacteria for the organic substrate. In this study, the effect of organic loading rate (OLR) on the H2 production performance, the metabolic pathways, and the microbial community composition in a continuous system was evaluated. Two bacterial genera, Clostridium and Streptococcus, were dominant in the microbial community depending on the OLR applied...
January 15, 2018: Applied Microbiology and Biotechnology
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