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https://www.readbyqxmd.com/read/27548814/retinoblastoma-binding-protein-2-rbp2-is-frequently-expressed-in-neuroendocrine-tumors-and-promotes-the-neoplastic-phenotype
#1
E C Maggi, J Trillo-Tinoco, A P Struckhoff, J Vijayaraghavan, L Del Valle, J S Crabtree
Neuroendocrine tumors (NETs), which can have survival rates as low as 4%, currently have limited therapeutic interventions available highlighting the dire need for the identification of novel biological targets for use as new potential drug targets. One such potential target is retinoblastoma-binding protein 2 (RBP2), an H3K4 demethylase whose overexpression has been linked to cancer formation and metastasis in non-endocrine tumor types. We measured RBP2 mRNA and protein levels in enteropancreatic NETs by measuring RBP2 in matched human normal and NET tissue samples...
2016: Oncogenesis
https://www.readbyqxmd.com/read/27453008/mir-34a-promotes-osteogenic-differentiation-of-human-adipose-derived-stem-cells-via-the-rbp2-notch1-cyclin-d1-coregulatory-network
#2
Cong Fan, Lingfei Jia, Yunfei Zheng, Chanyuan Jin, Yunsong Liu, Hao Liu, Yongsheng Zhou
MiR-34a was demonstrated to be upregulated during the osteogenic differentiation of human adipose-derived stem cells (hASCs). Overexpression of miR-34a significantly increased alkaline phosphatase activity, mineralization capacity, and the expression of osteogenesis-associated genes in hASCs in vitro. Enhanced heterotopic bone formation in vivo was also observed upon overexpression of miR-34a in hASCs. Mechanistic investigations revealed that miR-34a inhibited the expression of retinoblastoma binding protein 2 (RBP2) and reduced the luciferase activity of reporter gene construct comprising putative miR-34a binding sites in the 3' UTR of RBP2...
August 9, 2016: Stem Cell Reports
https://www.readbyqxmd.com/read/27449001/the-5-untranslated-region-of-the-rbp1-mrna-is-required-for-translation-of-its-mrna-under-low-temperatures-in-the-cyanobacterium-synechococcus-elongatus
#3
Rie Hayashi, Chieko Sugita, Mamoru Sugita
The unicellular cyanobacterium Synechococcus elongatus has three RNA-binding protein (Rbp) genes, rbp1, rbp2 and rbp3. The rbp1 gene was upregulated by cold treatment while rbp2 and rbp3 expression decreased remarkably after exposure to cold temperatures. To investigate the mechanism underlying cold-induced rbp1 expression, a series of rbp1-luxAB transcriptional fusion constructs were expressed in S. elongatus PCC 7942 under cold conditions. The results showed that the region from -33 to -3 of the transcription initiation site contains an essential sequence for basal transcription of the rbp1 gene and that the 120-bp region (-34 to -153) does not contain critical cis-elements required for cold-shock induction...
July 23, 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27282106/rbp2-induces-stem-like-cancer-cells-by-promoting-emt-and-is-a-prognostic-marker-for-renal-cell-carcinoma
#4
Dahai Zhou, Vinodh Kannappan, Xiang Chen, Jingqin Li, Xuefeng Leng, Jinping Zhang, Shiying Xuan
Renal cell carcinoma (RCC), one of the most common kidney cancers, has a poor prognosis. Epithelial to mesenchymal transition (EMT) is a hallmark of carcinoma invasion and metastasis. Several studies have examined the molecular regulation of EMT, but the relationship between histone demethylases and EMT is little understood. In this study, we investigated the role of retinoblastoma-binding protein-2 (RBP2), a histone demethylase that is highly expressed in RCC and is positively correlated with poor RCC prognosis in the regulation of EMT...
2016: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/27253695/depletion-of-histone-demethylase-jarid1a-resulting-in-histone-hyperacetylation-and-radiation-sensitivity-does-not-affect-dna-double-strand-break-repair
#5
Corina Penterling, Guido A Drexler, Claudia Böhland, Ramona Stamp, Christina Wilke, Herbert Braselmann, Randolph B Caldwell, Judith Reindl, Stefanie Girst, Christoph Greubel, Christian Siebenwirth, Wael Y Mansour, Kerstin Borgmann, Günther Dollinger, Kristian Unger, Anna A Friedl
Histone demethylases have recently gained interest as potential targets in cancer treatment and several histone demethylases have been implicated in the DNA damage response. We investigated the effects of siRNA-mediated depletion of histone demethylase Jarid1A (KDM5A, RBP2), which demethylates transcription activating tri- and dimethylated lysine 4 at histone H3 (H3K4me3/me2), on growth characteristics and cellular response to radiation in several cancer cell lines. In unirradiated cells Jarid1A depletion lead to histone hyperacetylation while not affecting cell growth...
2016: PloS One
https://www.readbyqxmd.com/read/27224921/screen-identified-selective-inhibitor-of-lysine-demethylase-5a-blocks-cancer-cell-growth-and-drug-resistance
#6
Molly Gale, Joyce Sayegh, Jian Cao, Michael Norcia, Peter Gareiss, Denton Hoyer, Jane S Merkel, Qin Yan
Lysine demethylase 5A (KDM5A/RBP2/JARID1A) is a histone lysine demethylase that is overexpressed in several human cancers including lung, gastric, breast and liver cancers. It plays key roles in important cancer processes including tumorigenesis, metastasis, and drug tolerance, making it a potential cancer therapeutic target. Chemical tools to analyze KDM5A demethylase activity are extremely limited as available inhibitors are not specific for KDM5A. Here, we characterized KDM5A using a homogeneous luminescence-based assay and conducted a screen of about 9,000 small molecules for inhibitors...
May 21, 2016: Oncotarget
https://www.readbyqxmd.com/read/27223670/super-elongation-complex-contains-a-tfiif-related-subcomplex
#7
Bruce A Knutson, Marissa L Smith, Nancy Walker-Kopp, Xia Xu
Super elongation complex (SEC) belongs to a family of RNA polymerase II (Pol II) elongation factors that has similar properties as TFIIF, a general transcription factor that increases the transcription elongation rate by reducing pausing. Although SEC has TFIIF-like functional properties, it apparently lacks sequence and structural homology. Using HHpred, we find that SEC contains an evolutionarily related TFIIF-like subcomplex. We show that the SEC subunit ELL interacts with the Pol II Rbp2 subunit, as expected for a TFIIF-like factor...
August 7, 2016: Transcription
https://www.readbyqxmd.com/read/27064087/antitumor-activities-and-immunomodulatory-of-rice-bran-polysaccharides-and-its-sulfates-in-vitro
#8
Li Wang, Yulin Li, Lidan Zhu, Ran Yin, Ren Wang, Xiaohu Luo, Yongfu Li, Yanan Li, Zhengxing Chen
Polysaccharides purified from rice bran show antitumor activity against tumor cells, yet the mechanism of this action remains poorly understood. To address this issue, our study evaluated the effect of rice bran polysaccharides on mouse melanoma cell line B16, and Raw264.7 macrophages. Rice bran polysaccharides (RBP) failed to inhibit B16 cell growth in vitro. However, Raw264.7 macrophages treated by RBP enhancement of cytotoxic effects. The cytotoxicity was confirmed by the stimulation of nitric oxide (NO) production and tumor necrosis factor-α (TNF-α) secretion on Raw264...
July 2016: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/27008505/rbp2-promotes-adult-acute-lymphoblastic-leukemia-by-upregulating-bcl2
#9
Xiaoming Wang, Minran Zhou, Yue Fu, Ting Sun, Jin Chen, Xuemei Qin, Yuan Yu, Jihui Jia, Chunyan Chen
Despite recent increases in the cure rate of acute lymphoblastic leukemia (ALL), adult ALL remains a high-risk disease that exhibits a high relapse rate. In this study, we found that the histone demethylase retinoblastoma binding protein-2 (RBP2) was overexpressed in both on-going and relapse cases of adult ALL, which revealed that RBP2 overexpression was not only involved in the pathogenesis of ALL but that its overexpression might also be related to relapse of the disease. RBP2 knockdown induced apoptosis and attenuated leukemic cell viability...
2016: PloS One
https://www.readbyqxmd.com/read/26788140/sirna-targeting-rbp2-inhibits-expression-proliferation-tumorigenicity-and-invasion-in-thyroid-carcinoma-cells
#10
Ling-Ling Kong, Dong-Mei Man, Tian Wang, Guo-An Zhang, Wen Cui
In order to estimate the effects of small interfering RNA (siRNA) targeting retinoblastoma binding protein 2 (RBP2) on the proliferation, expression, invasion, migration and tumorigenicity abilities of papillary thyroid carcinoma K1 cells, siRNA targeting RBP2 (RBP2-siRNA) and negative control siRNA were transfected into K1 cells. The mRNA levels of RBP2 in the transfected cells were estimated by reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and the protein levels of RBP2 in these cells were evaluated by western blot analysis and immunocytochemical (ICC) analyses...
December 2015: Oncology Letters
https://www.readbyqxmd.com/read/26645689/characterization-of-a-linked-jumonji-domain-of-the-kdm5-jarid1-family-of-histone-h3-lysine-4-demethylases
#11
John R Horton, Amanda Engstrom, Elizabeth L Zoeller, Xu Liu, John R Shanks, Xing Zhang, Margaret A Johns, Paula M Vertino, Haian Fu, Xiaodong Cheng
The KDM5/JARID1 family of Fe(II)- and α-ketoglutarate-dependent demethylases remove methyl groups from tri- and dimethylated lysine 4 of histone H3. Accumulating evidence from primary tumors and model systems supports a role for KDM5A (JARID1A/RBP2) and KDM5B (JARID1B/PLU1) as oncogenic drivers. The KDM5 family is unique among the Jumonji domain-containing histone demethylases in that there is an atypical insertion of a DNA-binding ARID domain and a histone-binding PHD domain into the Jumonji domain, which separates the catalytic domain into two fragments (JmjN and JmjC)...
February 5, 2016: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/26287723/the-rpb2-gene-represents-a-viable-alternative-molecular-marker-for-the-analysis-of-environmental-fungal-communities
#12
Tomáš Větrovský, Miroslav Kolařík, Lucia Žifčáková, Tomáš Zelenka, Petr Baldrian
Although the commonly used internal transcribed spacer region of rDNA (ITS) is well suited for taxonomic identification of fungi, the information on the relative abundance of taxa and diversity is negatively affected by the multicopy nature of rDNA and the existence of ITS paralogues. Moreover, due to high variability, ITS sequences cannot be used for phylogenetic analyses of unrelated taxa. The part of single-copy gene encoding the second largest subunit of RNA polymerase II (rpb2) was thus compared with first spacer of ITS as an alternative marker for the analysis of fungal communities in spruce forest topsoil, and their applicability was tested on a comprehensive mock community...
March 2016: Molecular Ecology Resources
https://www.readbyqxmd.com/read/26264242/retinoblastoma-binding-protein-2-induces-epithelial-mesenchymal-transition-in-esophageal-squamous-cancer-cells
#13
Naian Qiao, Shikun Wang, Likuan Hu
OBJECTIVES: To investigate the effect of retinoblastoma-binding protein 2 (RBP2) on epithelial-mesenchymal transition (EMT) in esophageal squamous cancer cells and to compare the effect of RBP2 in lung squamous cancer cells and esophageal squamous cancer cells. RESULTS: When transfected with RBP2 siRNA, the migrated cells were 36.3 ± 6.03 by transwell migration assay, compared to 107 ± 6.7 cells in the control group. The mRNA level of epithelial cadherin (E-cadherin) was 1...
December 2015: Biotechnology Letters
https://www.readbyqxmd.com/read/25974964/histone-demethylase-rbp2-promotes-malignant-progression-of-gastric-cancer-through-tgf-%C3%AE-1-p-smad3-rbp2-e-cadherin-smad3-feedback-circuit
#14
Xiuming Liang, Jiping Zeng, Lixiang Wang, Li Shen, Xueping Ma, Shuyan Li, Yujiao Wu, Lin Ma, Xinyu Ci, Qing Guo, Mutian Jia, Haiyu Shen, Yundong Sun, Zhifang Liu, Shili Liu, Wenjuan Li, Han Yu, Chunyan Chen, Jihui Jia
Some feedback pathways are critical in the process of tumor development or malignant progression. However the mechanisms through which these pathways are epigenetically regulated have not been fully elucidated. Here, we demonstrated that the histone demethylase RBP2 was crucial for TGF-β1-(p-Smad3)-RBP2-E-cadherin-Smad3 feedback circuit that was implicated in malignant progression of tumors and its knockdown significantly inhibited gastric cancer (GC) metastasis both in vitro and in vivo. Mechanistically, RBP2 can directly bind to E-cadherin promoter and suppress its expression, facilitating EMT and distant metastasis of GC...
July 10, 2015: Oncotarget
https://www.readbyqxmd.com/read/25789538/altered-splicing-of-jarid1b-in-development-of-human-cutaneous-melanoma
#15
Łukasz Kuźbicki, Dariusz Lange, Anita Strączyńska-Niemiec, Barbara W Chwirot
Upregulated expression of histone H3K4 demethylase JARID1B has been found in several types of human cancer, but the expression pattern of this protein in benign naevi and human cutaneous melanomas seems to differ from that described for other tumors. We demonstrate that the apparent contradiction may be because of the fact that the malignant transformation of melanocytes is associated not so much with a general enhancement of a total expression of JARID1B but rather with a change in relative expression levels of individual splicing variants of the protein...
March 2016: Applied Immunohistochemistry & Molecular Morphology: AIMM
https://www.readbyqxmd.com/read/25590587/characterization-of-plasmodium-ovale-curtisi-and-p-ovale-wallikeri-in-western-kenya-utilizing-a-novel-species-specific-real-time-pcr-assay
#16
Robin H Miller, Clifford O Obuya, Elizabeth W Wanja, Bernhards Ogutu, John Waitumbi, Shirley Luckhart, V Ann Stewart
BACKGROUND: Plasmodium ovale is comprised of two genetically distinct subspecies, P. ovale curtisi and P. ovale wallikeri. Although P. ovale subspecies are similar based on morphology and geographical distribution, allelic differences indicate that P. ovale curtisi and P. ovale wallikeri are genetically divergent. Additionally, potential clinical and latency duration differences between P. ovale curtisi and P. ovale wallikeri demonstrate the need for investigation into the contribution of this neglected malaria parasite to the global malaria burden...
January 2015: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/25585692/massive-bowel-resection-upregulates-the-intestinal-mrna-expression-levels-of-cellular-retinol-binding-protein-ii-and-apolipoprotein-a-iv-and-alters-the-intestinal-vitamin-a-status-in-rats
#17
Taku Hebiguchi, Yoshihiro Mezaki, Mayako Morii, Ryo Watanabe, Kiwamu Yoshikawa, Mitsutaka Miura, Katsuyuki Imai, Haruki Senoo, Hiroaki Yoshino
Short bowel (SB) syndrome causes the malabsorption of various nutrients. Among these, vitamin A is important for a number of physiological activities. Vitamin A is absorbed by epithelial cells of the small intestine and is discharged into the lymphatic vessels as a component of chylomicrons and is delivered to the liver. In the present study, we used a rat model of SB syndrome in order to assess its effects on the expression of genes associated with the absorption, transport and metabolism of vitamin A. In the rats with SB, the intestinal mRNA expression levels of cellular retinol-binding protein II (CRBP II, gene symbol Rbp2) and apolipoprotein A-IV (gene symbol Apoa4) were higher than those in the sham-operated rats, as shown by RT-qPCR...
March 2015: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/25575817/histone-demethylase-rbp2-decreases-mir-21-in-blast-crisis-of-chronic-myeloid-leukemia
#18
Minran Zhou, Jiping Zeng, Xiaoming Wang, Xiangyu Wang, Tao Huang, Yue Fu, Ting Sun, Jihui Jia, Chunyan Chen
Chronic myeloid leukemia in the blastic phase (CML-BP) responds poorly to clinical treatments and is usually fatal. In this study, we found that the histone H3 lysine 4 (H3K4) demethylase RBP2 (also called JARID1A and KDM5A) is underexpressed in CML-BP. The RBP2 histone demethylase stimulates leukemia cell differentiation and inhibits cell proliferation. We identified miR-21 was directly downregulated by RBP2 and found that miR-21 downregulated PDCD4 expression in leukemia cells. By binding to miR-21 promoter and by demethylating of trimethylated H3K4 at the miR-21 locus, RBP2 downregulated miR-21 expression...
January 20, 2015: Oncotarget
https://www.readbyqxmd.com/read/25537453/dynamic-epigenetic-regulation-by-menin-during-pancreatic-islet-tumor-formation
#19
Wenchu Lin, Hideo Watanabe, Shouyong Peng, Joshua M Francis, Nathan Kaplan, Chandra Sekhar Pedamallu, Aruna Ramachandran, Agoston Agoston, Adam J Bass, Matthew Meyerson
UNLABELLED: The tumor suppressor gene MEN1 is frequently mutated in sporadic pancreatic neuroendocrine tumors (PanNET) and is responsible for the familial multiple endocrine neoplasia type 1 (MEN-1) cancer syndrome. Menin, the protein product of MEN1, associates with the histone methyltransferases (HMT) MLL1 (KMT2A) and MLL4 (KMT2B) to form menin-HMT complexes in both human and mouse model systems. To elucidate the role of methylation of histone H3 at lysine 4 (H3K4) mediated by menin-HMT complexes during PanNET formation, genome-wide histone H3 lysine 4 trimethylation (H3K4me3) signals were mapped in pancreatic islets using unbiased chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq)...
April 2015: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/25468371/the-nanoscale-geometry-of-tio2-nanotubes-influences-the-osteogenic-differentiation-of-human-adipose-derived-stem-cells-by-modulating-h3k4-trimethylation
#20
Longwei Lv, Yunsong Liu, Ping Zhang, Xiao Zhang, Jianzhang Liu, Tong Chen, Penglei Su, Hongyi Li, Yongsheng Zhou
Nanostructured materials can direct stem cell lineage commitment solely by their various, but controllable, geometric cues, which would be very important for their future application in bone tissue engineering and bone regeneration. However, the mechanisms by which nano-geometric cues dictate the osteogenic differentiation of stem cells remain unclear. Epigenetics is central to cellular differentiation, a process that regulates heritable and long-lasting alterations in gene expression without changing the DNA sequence...
January 2015: Biomaterials
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