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https://www.readbyqxmd.com/read/29786079/dissection-of-dna-double-strand-break-repair-using-novel-single-molecule-forceps
#1
Jing L Wang, Camille Duboc, Qian Wu, Takashi Ochi, Shikang Liang, Susan E Tsutakawa, Susan P Lees-Miller, Marc Nadal, John A Tainer, Tom L Blundell, Terence R Strick
Repairing DNA double-strand breaks (DSBs) by nonhomologous end joining (NHEJ) requires multiple proteins to recognize and bind DNA ends, process them for compatibility, and ligate them together. We constructed novel DNA substrates for single-molecule nanomanipulation, allowing us to mechanically detect, probe, and rupture in real-time DSB synapsis by specific human NHEJ components. DNA-PKcs and Ku allow DNA end synapsis on the 100 ms timescale, and the addition of PAXX extends this lifetime to ~2 s. Further addition of XRCC4, XLF and ligase IV results in minute-scale synapsis and leads to robust repair of both strands of the nanomanipulated DNA...
May 21, 2018: Nature Structural & Molecular Biology
https://www.readbyqxmd.com/read/29784126/endobronchial-forceps-assisted-and-excimer-laser-assisted-inferior-vena-cava-filter-removal-the-data-where-we-are-and-how-it-is-done
#2
James X Chen, Jennifer Montgomery, Gordon McLennan, S William Stavropoulos
The recognition of inferior vena cava filter related complications has motivated increased attentiveness in clinical follow-up of patients with inferior vena cava filters and has led to development of multiple approaches for retrieving filters that are challenging or impossible to remove using conventional techniques. Endobronchial forceps and excimer lasers are tools for designed to aid in complex inferior vena cava filter removals. This article discusses endobronchial forceps-assisted and excimer laser-assisted inferior vena cava filter retrievals...
June 2018: Techniques in Vascular and Interventional Radiology
https://www.readbyqxmd.com/read/29780141/hemorrhagic-stress-induced-gastric-ulcer-in-a-healthy-toddler
#3
Katsunori Matsueda, Tatsuya Toyokawa, Masahiro Sakata, Isao Fujita, Jouichiro Horii, Yoshiko Abe, Toru Araki
A 1-year-old female toddler who was under a great amount of psychological stress presented with acute hematemesis. She had no significant medical history; she displayed lethargy and was anemic. Although blood transfusion and famotidine were administered for upper gastrointestinal bleeding (UGIB), the anemia did not improve. Thus, early endoscopy was performed under general anesthesia, revealing a gastric ulcer with exposed vessels in the supra-angular region. Cauterization via bipolar hemostatic forceps was performed on the exposed vessels, and the bleeding was controlled successfully...
May 18, 2018: Internal Medicine
https://www.readbyqxmd.com/read/29780022/a-case-of-felicola-subrostratus-burmeister-1838-phthiraptera-ischnocera-on-a-cat-felis-catus
#4
Bilal Dik
We present this case report to provide information about Felicola subrostratus (Burmeister, 1838) (Phthiraptera: Ischnocera) detected on a 2-year-old stray cat (Felis catus) brought for treatment to a veterinary clinic in Kocaeli, Turkey. Macroscopically, severe louse infestation was detected on the cat. Some of the lice on the cat were collected using forceps and preserved in 70% ethanol. Then, they were sent to Department of Parasitology, Veterinary Faculty, Selçuk University in Konya for identification...
March 2018: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29780020/a-rare-case-of-blebharitis-phthiriasis-palpebrarum
#5
Fatma Altınsoy, Oktay Alver, Selim Doğanay
A six-year-old male patient was admitted to our hospital due to itching and scalding crusts that persisted 10-15 days in both eyes. Upon biomicroscopic examination, 5-6 semi-translucent, yellowish brown living lice attached to the upper eyelashes and a large number of eggs were observed. Following application of pilocarpine hydrochloride (Pilomann 2%, Bausch-Lomb) and topical proparacaine hydrochloride (Alcaine 0.5%, Alcon), the paralyzed parasites and eggs were manually removed by pulling with forceps. The lice were identified as adult forms of pubic louse, Pthirus pubis, and its eggs...
March 2018: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29779498/retrieval-of-large-occlutech-figula-flex-septal-defect-occluders-using-a-commercially-available-bioptome-proof-of-concept
#6
Stanimir Georgiev, Daniel Tanase, Thomas Genz, Peter Ewert, Susanne Naumann, Robert Dalla Pozza, Andreas Eicken
OBJECTIVES: This study aimed to develop a method for retrieval of the new meshed nitinol atrial septal defect occluders - Ceraflex and Occlutech. BACKGROUND: The newly developed atrial septal defect occluders have potential benefits considering implantation, but concerns regarding their removal in case of embolisation have been raised. METHODS: Over 21 years, 1449 patients underwent interventional atrial septal defect occlusion in our institution...
May 21, 2018: Cardiology in the Young
https://www.readbyqxmd.com/read/29779238/the-role-of-transbronchial-cryobiopsy-in-lung-transplantation
#7
M Angeles Montero, Javier de Gracia, Mario Culebras, Jacqueline Mugnier, Antonio Álvarez, Cristina Berastegui, Cristian Ortiz-Villalón
INTRODUCTION: Lung transplant monitoring is usually assessed by forceps transbronchial biopsies. These types of biopsies show limited reliability and high degree of variability due to insufficient material and compression artefact, which lead to misinterpretation and eventually mistreatment of the transplanted patients. The following study was undertaken to assess the diagnostic yield, histological quality and safety of cryobiopsy in comparison with conventional forceps biopsy for sampling lung tissue in transplant recipients...
May 19, 2018: Histopathology
https://www.readbyqxmd.com/read/29771844/an-innovative-and-easy-dome-holding-technique-for-transdomal-suture-in-rhinoplasty
#8
Sureyya Seneldir, Yusuf Muhammed Durna
Suture tip plasty is one the most used methods in nasal tip surgery. Transdomal suture (TDS) used for this purpose is utilized for providing the dome symmetry, thinning the nasal tip, and enhancing the nasal tip projection and rotation. However, there are not sufficient visual data on what the dome position should be during TDS. In this study, it was aimed to be directive in tip plasty and to define the convenient dome holding technique during TDS. Patients, 510 male and 621 female at the age of 18 to 50 years, who underwent the open technique septorhinoplasty between the years of 2003 to 2015 were included in the authors' study...
May 15, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/29770623/quality-of-tissue-from-punch-biopsy-forceps-vs-round-loop-electrode-in-colposcopically-directed-biopsy-a-randomized-controlled-trial
#9
Thanita Wetcho, Athithan Rattanaburi, Kanet Kanjanapradit
OBJECTIVE: To compare the quality of tissue from punch biopsy forceps (PB group) with round loop electrode (LE group) in colposcopically directed biopsy along with the evaluation of pain associated with each procedure. METHODS: Patients with abnormal cervical cytologic results and abnormal colposcopic findings were enrolled into a randomized trial into either a PB group or LE group. The quality of tissue was evaluated in regards to the size of tissue, site of tissue, and tissue damage...
March 12, 2018: Journal of Gynecologic Oncology
https://www.readbyqxmd.com/read/29770435/fetal-rotation-during-vacuum-extractions-for-prolonged-labour-a-prospective-cohort-study
#10
Birgitte Heiberg Kahrs, Sana Usman, Tullio Ghi, Aly Youssef, Erik Andreas Torkildsen, Elsa Lindtjørn, Tilde Borch Østborg, Sigurlaug Benediktsdottir, Lis Brooks, Lotte Harmsen, Kjell Åsmund Salvesen, Cristoph C Lees, Torbjørn Moe Eggebø
INTRODUCTION: The aim of the study was to investigate fetal head rotation during vacuum extraction. MATERIAL AND METHODS: We conducted a prospective cohort study from November 2013 to July 2016 in seven European hospitals. Fetal head position was determined with transabdominal or transperineal ultrasound and categorized as occiput anterior (OA), occiput transverse (OT) or occiput posterior (OP) position. Main outcome was the proportion of fetuses rotating during vacuum extraction...
May 16, 2018: Acta Obstetricia et Gynecologica Scandinavica
https://www.readbyqxmd.com/read/29764299/efficacy-of-sufficient-operation-view-by-ring-shaped-thread-counter-traction-for-safer-duodenal-esd
#11
Yasuhiro Goda, Hirohito Mori, Hideki Kobara, Tsutomu Masaki
BACKGROUND: Duodenal ESD is considered especially difficult with perforation and bleeding. This study assessed safer duodenal ESD procedures, especially with regard to obtaining a good operation view using a ring-thread method and closure of a post-ESD artificial ulcer. METHODS: From 2013 to 2015, 17 patients who were diagnosed with duodenal adenoma or early duodenal cancer >20 mm in diameter underwent conventional ESD (C group). From 2016 to 2017, 12 patients underwent ring-shaped thread counter traction ESD with hemoclips and/or Over-The-Scope Clip (OTSC) (Ovesco Endoscopy GmbH, Tuebingen, Germany) closure of post ESD artificial ulcer (ring group)...
May 15, 2018: Minimally Invasive Therapy & Allied Technologies: MITAT
https://www.readbyqxmd.com/read/29760741/establishing-a-surgical-procedure-for-rhesus-epiretinal-scaffold-implantation-with-hipsc-derived-retinal-progenitors
#12
Ziming Luo, Kang Li, Kaijing Li, Bikun Xian, Ying Liu, Sijing Yang, Chaochao Xu, Zhigang Fan, Shoutao Lu, Haijun Zhang, Jian Ge
Background: To develop an effective surgical procedure for cellular scaffold epiretinal implantation in rhesus, facilitating subsequent epiretinal stem cell transplantation. Methods: Retinal progenitors were seeded onto a poly(lactic-co-glycolic) acid (PLGA) scaffold. First, the cellular scaffolds were delivered by 18G catheter or retinal forceps into rabbit epiretinal space ( n = 50). Then, the cell survival rate was evaluated by Cell Counting Kit-8 (CCK-8). Second, three methods of scaffold fixation, including adhesion after gas-liquid exchange ( n = 1), tamponade by hydrogel ( n = 1), and fixation by retinal tacks ( n = 4), were performed in rhesus monkeys...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29756483/the-glidescope-with-modified-magill-forceps-facilitates-nasogastric-tube-insertion-in-anesthetized-patients-a-randomized-clinical-study
#13
Han Joon Kim, Su In Park, Sang Yun Cho, Min Jae Cho
Objective Insertion of a nasogastric tube (NGT) in patients who have been intubated with an endotracheal tube while under general anesthesia can cause difficulties and lead to complications, including hemorrhage. A visualization-aided modality was recently used to facilitate NGT insertion. Some studies have focused on the role of modified Magill forceps, which have angles similar to those of the GlideScope blade (Verathon, Bothell, WA, USA). Methods Seventy patients were divided into a control group (Group C) and an experimental group (GlideScope and modified Magill forceps, Group M)...
January 1, 2018: Journal of International Medical Research
https://www.readbyqxmd.com/read/29756018/a-structured-light-laser-probe-for-gastrointestinal-polyp-size-measurement-a-preliminary-comparative-study
#14
Marco Visentini-Scarzanella, Hiroshi Kawasaki, Ryo Furukawa, Marco Augusto Bonino, Simone Arolfo, Giacomo Lo Secco, Alberto Arezzo, Arianna Menciassi, Paolo Dario, Gastone Ciuti
Background and study aims:  Polyp size measurement is an important diagnostic step during gastrointestinal endoscopy, and is mainly performed by visual inspection. However, lack of depth perception and objective reference points are acknowledged factors contributing to measurement errors in polyp size. In this paper, we describe the proof-of-concept of a polyp measurement device based on structured light technology for future endoscopes. Patients and methods:  Measurement accuracy, time, user confidence, and satisfaction were evaluated for polyp size assessment by (a) visual inspection, (b) open biopsy forceps of known size, (c) ruled snare, and (d) structured light probe, for a total of 392 independent polyp measurements in ex vivo porcine stomachs...
May 2018: Endoscopy International Open
https://www.readbyqxmd.com/read/29753476/technical-note-the-development-of-a-methodology-for-ruminal-and-colon-tissue-biopsying-of-young-holstein-dairy-calves
#15
J K van Niekerk, M Middeldorp, M A Steele
The objectives of this study were to develop a methodology for biopsying the rumen and colon of young dairy calves and to collect suitable quality tissue samples for microscopic and gene expression analysis. Six Holstein dairy bull calves (45.0 ± 1.5 kg birth weight) were ruminally cannulated during the second week of life and weaned at the end of wk 6. Ruminal and colon tissue samples were collected at the end of wk 5, 6, 7, 8, and 12. Calves were not sedated but were restrained in a chute for sampling. The endoscope (100 cm length, 9...
May 9, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29750926/comparative-safety-and-efficacy-of-a-dedicated-postpartum-iud-inserter-versus-forceps-for-immediate-postpartum-iud-insertion-a-randomized-trial
#16
Paul D Blumenthal, Klaira Lerma, Renita Bhamrah, Sharad Singh
OBJECTIVE: To compare postpartum IUD (PPIUD) insertion using a newly developed dedicated PPIUD inserter (inserter) to insertion with modified Kelly placental forceps (forceps). STUDY DESIGN: We randomized 500 women at five Indian academic centers to PPIUD insertion of a Copper T380A with either the inserter or forceps. The primary outcomes were fundal placement (assessed by post-insertion ultrasound), ease of insertion on a 5-point Likert scale, and safety (infection/perforation)...
May 8, 2018: Contraception
https://www.readbyqxmd.com/read/29750618/modified-approach-to-central-giant-cell-lesion
#17
Lucas Borin Moura, Sandra Beatriz Chaves Tarquinio, Ana Paula Neutzling Gomes, André Ribeiro Schinestsck, Marcos Antonio Torriani
This case report discusses a modified technique for treatment of a central giant cell lesion (CGCL) in children, where a recontouring procedure is performed prior to the treatment with intra lesional corticosteroid. We discuss the advantages of this less invasive technique, specially considering the early age of the patient and its bone growth, as well as the conservative approach for lesions in those cases. The treatment of an 8-year-old female patient exhibiting CGCL in anterior region of mandible with bone expansion is described...
May 11, 2018: Journal of Clinical Pediatric Dentistry
https://www.readbyqxmd.com/read/29737732/-clinical-analysis-of-arytenoid-cartilage-reposition-with-snake-mouth-forceps-for-the-arytenoid-cartilage-dislocation
#18
Qing Xiang Zhang, Shuang Ba He, Zi Gang Che, Hui Ying Hu, Ya Qun Liu, Yuan Yuan Lu, Zhen Kun Yu
OBJECTIVES: To investigate the clinical effect of the arytenoid cartilage reposition using snake mouth reduction forceps under general anesthesia. METHODS: Data of twenty-six cases accepted arytenoid cartilage reposition under intravenous general anesthesia were analyzed, nineteen cases accepted laryngeal CT scan and cricoarytenoid joint reconstruction, all patients underwent endolaryngeal muscle electromyography examination. According to the position of cartilage dislocation prompted by laryngoscope and CT, the arytenoid cartilage was repositoned under the visual laryngoscope using special snake mouth reduction forceps...
March 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
https://www.readbyqxmd.com/read/29731123/the-improved-oval-forceps-suture-guiding-method-for-minimally-invasive-achilles-tendon-repair
#19
Yang Liu, Lixiang Lin, Chuanlu Lin, Qihao Weng, Jianjun Hong
OBJECTIVE: To discuss the effect and advantage of the improved oval forceps suture-guiding method combined with anchor nail in the treatment of acute Achilles tendon rupture. METHODS: A retrospective research was performed on 35 cases of acute Achilles tendon rupture treated with the improved oval forceps suture-guiding method from January 2013 to October 2016. Instead of the Achillon device, we perform the Achillon technique with the use of simple oval forceps, combined with absorbable anchor nail, percutaneously to repair the acute Achilles tendon rupture...
May 2, 2018: Injury
https://www.readbyqxmd.com/read/29723878/biomechanical-comparison-between-preloaded-position-screw-and-lag-screw-fixations-for-their-compressive-effects-in-a-porcine-rib-fracture-model
#20
Ya-Pei Chang, Chi-Yuan Ho, Chao-Chang Chen, Lih-Seng Yeh
OBJECTIVES:  The aim of this study was to compare the compressive effect between preloaded position screws and lag screws in fracture fixation. METHODS:  Pairs of semi-cylindrical bone fragments were created on a porcine rib model to simulate fractured bones. The compressive forces of fracture fixation generated by preloaded position screws (P group) and conventional lag screws (L group) were recorded by a film stress sensor. In the P group, a pair of Angus bone holding forceps was used to compress the interfragmentary interface until reaching the preloading force of 1...
May 3, 2018: Veterinary and Comparative Orthopaedics and Traumatology: V.C.O.T
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