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Intracardiac cell transplantation

Rui Wei, Jin Yang, Meijuan Gao, Haining Wang, Wenfang Hou, Yiming Mu, Guian Chen, Tianpei Hong
Microenvironment regulates cell fate and function. In this study, we investigated the effects of the infarcted cardiac microenvironment on cardiac differentiation of human embryonic stem cells (hESCs). hESCs were intramyocardially transplanted into infarcted or uninjured rat hearts. After 4 weeks, mesodermal and cardiac lineage markers were detected by immunofluorescence. Cardiac function was assessed by echocardiography. hESCs were differentiated in vitro under hypoxic (5% O2 ), low-nutrient (5% FBS), or control condition...
November 2016: Cell Biology International
Natesh Parashurama, Byeong-Cheol Ahn, Keren Ziv, Ken Ito, Ramasamy Paulmurugan, Jürgen K Willmann, Jaehoon Chung, Fumiaki Ikeno, Julia C Swanson, Denis R Merk, Jennifer K Lyons, David Yerushalmi, Tomohiko Teramoto, Hisanori Kosuge, Catherine N Dao, Pritha Ray, Manishkumar Patel, Ya-Fang Chang, Morteza Mahmoudi, Jeff Eric Cohen, Andrew Brooks Goldstone, Frezghi Habte, Srabani Bhaumik, Shahriar Yaghoubi, Robert C Robbins, Rajesh Dash, Phillip C Yang, Todd J Brinton, Paul G Yock, Michael V McConnell, Sanjiv S Gambhir
Purpose To quantitatively determine the limit of detection of marrow stromal cells (MSC) after cardiac cell therapy (CCT) in swine by using clinical positron emission tomography (PET) reporter gene imaging and magnetic resonance (MR) imaging with cell prelabeling. Materials and Methods Animal studies were approved by the institutional administrative panel on laboratory animal care. Seven swine received 23 intracardiac cell injections that contained control MSC and cell mixtures of MSC expressing a multimodality triple fusion (TF) reporter gene (MSC-TF) and bearing superparamagnetic iron oxide nanoparticles (NP) (MSC-TF-NP) or NP alone...
September 2016: Radiology
Wen-Chi Yang, Ming Yao, Yu-Hsuan Chen, Sung-Hsin Kuo
We present a rare case of intracardiac myeloid sarcoma (MS) of acute myeloid leukemia (AML) and who responds completely well to low-dose radiotherapy. This 19-year-old young man initially presented with AML and received standard chemotherapy followed by allogeneic hematopoietic stem cell transplantation (HSCT). However, he developed intracardiac isolated MS relapse with the presentation of exertional dyspnea and superior vena cava (SVC) syndrome 3 years later. He then received radiotherapy with 24 Gy at a 12 daily fractions using forward "field in field" intensity modulated radiotherapy technique...
June 2016: Journal of Thoracic Disease
Karin Boer, Kadir Caliskan, Annemiek M A Peeters, Marian C van Groningen, Janneke N Samsom, Alexander P W M Maat, Michiel G H Betjes, Willem Weimar, Carla C Baan
BACKGROUND: FOXP3+ regulatory T cells (Treg) either originate in the thymus (natural [n]Treg) or are induced in the periphery by antigen exposure and cytokines (induced [i]Treg). It is currently not elucidated which and to what extent these Treg subsets regulate intracardiac allogeneic responses in transplant patients. METHODS: By using demethylation of the Treg-specific demethylated region in the FOXP3 gene as a marker for nTreg and FOXP3 messenger RNA expression as a marker for the total Treg population, we examined Treg in endomyocardial biopsies (EMBs) of both patients who developed an acute rejection necessitating therapy (rejectors; International Society for Heart and Lung Transplantation rejection grade ≥ 2R) and patients who remained free from rejection (nonrejectors)...
September 2015: Transplantation
Prabhjot Singh Nijjar, Sofia Carolina Masri, Ashenafi Tamene, Helina Kassahun, Kenneth Liao, Uma Valeti
Primary cardiac tumors are far rarer than tumors metastatic to the heart. Angiosarcoma is the primary cardiac neoplasm most frequently detected; lymphomas constitute only 1% of primary cardiac tumors. We present the case of a 55-year-old woman with a recently diagnosed intracardiac mass who was referred to our institution for consideration of urgent orthotopic heart transplantation. Initial images suggested an angiosarcoma; however, a biopsy specimen of the mass was diagnostic for diffuse large B-cell lymphoma...
December 2014: Texas Heart Institute Journal
Paula H Suss, Luiz Guilherme A Capriglione, Fabiane Barchiki, Lye Miyague, Danielle Jackowski, Letícia Fracaro, Andressa V Schittini, Alexandra C Senegaglia, Carmen L K Rebelatto, Márcia Olandoski, Alejandro Correa, Paulo R S Brofman
The development of new therapeutic strategies is necessary to reduce the worldwide social and economic impact of cardiovascular disease, which produces high rates of morbidity and mortality. A therapeutic option that has emerged in the last decade is cell therapy. The aim of this study was to compare the effect of transplanting human umbilical cord-derived stromal cells (UCSCs), human umbilical cord blood-derived endothelial cells (UCBECs) or a combination of these two cell types for the treatment of ischemic cardiomyopathy (IC) in a Wistar rat model...
July 2015: Experimental Biology and Medicine
Fang-Yuan Wei, Kwok-Sui Leung, Gang Li, Jianghui Qin, Simon Kwoon-Ho Chow, Shuo Huang, Ming-Hui Sun, Ling Qin, Wing-Hoi Cheung
Low intensity pulsed ultrasound (LIPUS) has been proven effective in promoting fracture healing but the underlying mechanisms are not fully depicted. We examined the effect of LIPUS on the recruitment of mesenchymal stem cells (MSCs) and the pivotal role of stromal cell-derived factor-1/C-X-C chemokine receptor type 4 (SDF-1/CXCR4) pathway in response to LIPUS stimulation, which are essential factors in bone fracture healing. For in vitro study, isolated rat MSCs were divided into control or LIPUS group. LIPUS treatment was given 20 minutes/day at 37 °C for 3 days...
2014: PloS One
Hv New, Ce Chapman, Md Kilby, S Kumar, Cj Brown, Phb Bolton-Maggs
: For intrauterine transfusion (IUT) specific red cells are provided by UK Blood Services, irradiated to prevent TA-GvHD. A fetus (21/40) was referred to a fetal medicine centre (FMC), severely anaemic (parvovirus) requiring urgent IUT. Maternal blood was transfused to fetus. Following poor cardiac output, further emergency intracardiac transfusion gave improvement. At 32/40 baby was delivered, severely hydropic and pancytopenic (aplasia later confirmed by marrow aspirate). Hyperbilirubinaemia and fungal chest infection followed...
June 2014: Archives of Disease in Childhood. Fetal and Neonatal Edition
Jesse D Vrecenak, Erik G Pearson, Matthew T Santore, Carlyn A Todorow, Haiying Li, Antoneta Radu, Tricia Bhatti, William H Peranteau, Mark P Johnson, Alan W Flake
Evidence supporting the efficacy of in utero hematopoietic cell transplantation (IUHCT) in a valid large animal model is needed prior to clinical application. The objective of this study was to establish clinically relevant levels of hematopoietic chimerism in a canine model of maternal-to-fetal IUHCT. We first assessed immune and hematopoietic ontogeny relevant to IUHCT in the canine model and identified 40 days' gestation (term 63 days) as a time point at the initiation of thymic selection, and prior to bone marrow hematopoiesis, that might be optimal for IUHCT...
September 18, 2014: Blood
Andreas Martens, Sebastian V Rojas, Hassina Baraki, Christian Rathert, Natalie Schecker, Robert Zweigerdt, Kristin Schwanke, Sara Rojas-Hernandez, Ulrich Martin, Shunsuke Saito, Jan D Schmitto, Axel Haverich, Ingo Kutschka
The limited success of cardiac stem cell therapy has lately generated discussion regarding its effectiveness. We hypothesized that immediate cell loss after intramyocardial injection significantly obscures the regenerative potential of stem cell therapy. Therefore, our aim was to assess the distribution and quantity of induced pluripotent stem cells after intramyocardial delivery using in vivo bioluminescence analysis. In this context, we wanted to investigate if the injection of different cell concentrations would exert influence on cardiac cell retention...
November 2014: Artificial Organs
Yuji Shiba, Dominic Filice, Sarah Fernandes, Elina Minami, Sarah K Dupras, Benjamin Van Biber, Peter Trinh, Yusuke Hirota, Joseph D Gold, Mohan Viswanathan, Michael A Laflamme
BACKGROUND: Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) were recently shown to be capable of electromechanical integration following direct injection into intact or recently injured guinea pig hearts, and hESC-CM transplantation in recently injured hearts correlated with improvements in contractile function and a reduction in the incidence of arrhythmias. The present study was aimed at determining the ability of hESC-CMs to integrate and modulate electrical stability following transplantation in a chronic model of cardiac injury...
February 10, 2014: Journal of Cardiovascular Pharmacology and Therapeutics
Anne-Marie Bleau, Javier Freire, María José Pajares, Isabel Zudaire, Iker Anton, Estanislao Nistal-Villán, Miriam Redrado, Caroli Na Zandueta, Irati Garmendia, Daniel Ajona, David Blanco, Ruben Pio, Fernando Lecanda, Alfonso Calvo, Luis M Montuenga
New mouse models with specific drivers of genetic alterations are needed for preclinical studies. Herein, we created and characterized at the genetic level a new syngeneic model for lung cancer and metastasis in Balb-c mice. Tumor cell lines were obtained from a silica-mediated airway chronic inflammation that promotes tumorigenesis when combined with low doses of N-nitrosodimethylamine, a tobacco smoke carcinogen. Orthotopic transplantation of these cells induced lung adenocarcinomas, and their intracardiac injection led to prominent colonization of various organs (bone, lung, liver and brain)...
December 1, 2014: International Journal of Cancer. Journal International du Cancer
Tomasz B Owczarek, Jarosław Suchanski, Bartosz Pula, Alicja M Kmiecik, Marek Chadalski, Aleksandra Jethon, Piotr Dziegiel, Maciej Ugorski
It was recently proposed that UDP-galactose:ceramide galactosyltransferase (UGT8), enzyme responsible for synthesis of galactosylceramide (GalCer), is a significant index of tumor aggressiveness and a potential marker for the prognostic evaluation of lung metastases in breast cancer. To further reveal the role of UGT8 and GalCer in breast cancer progression, tumorigenicity and metastatic potential of control MDA-MB-231 cells (MDA/LUC) and MDA-MB-231 cells (MDA/LUC-shUGT8) with highly decreased expression of UGT8 and GalCer after stable expression of shRNA directed against UGT8 mRNA was studied in vivo in athymic nu/nu mice...
2013: PloS One
Massimo Muratore, Vlastimil Srsen, Martin Waterfall, Andrew Downes, Ronald Pethig
Myoblasts are muscle derived mesenchymal stem cell progenitors that have great potential for use in regenerative medicine, especially for cardiomyogenesis grafts and intracardiac cell transplantation. To utilise such cells for pre-clinical and clinical applications, and especially for personalized medicine, it is essential to generate a synchronised, homogenous, population of cells that display phenotypic and genotypic homogeneity within a population of cells. We demonstrate that the biomarker-free technique of dielectrophoresis (DEP) can be used to discriminate cells between stages of differentiation in the C2C12 myoblast multipotent mouse model...
September 2012: Biomicrofluidics
Kara White Moyes, Christopher G Sip, Willimark Obenza, Emily Yang, Cody Horst, Robert E Welikson, Stephen D Hauschka, Albert Folch, Michael A Laflamme
An improved understanding of the factors that regulate the migration of human embryonic stem cell-derived cardiomyocytes (hESC-CMs) would provide new insights into human heart development and suggest novel strategies to improve their electromechanical integration after intracardiac transplantation. Since nothing has been reported as to the factors controlling hESC-CM migration, we hypothesized that hESC-CMs would migrate in response to the extracellular matrix and soluble signaling molecules previously implicated in heart morphogenesis...
August 15, 2013: Stem Cells and Development
Jesse V Jokerst, Christine Khademi, Sanjiv S Gambhir
The promises of cardiac stem cell therapy have yet to be fully realized, in part because of poor survival and engraftment efficacy of implanted cells. Cells die after implantation owing to ischemia, inflammation, immune response, as well as mis-injection or implantation into fibrotic tissue. Imaging tools can help implant cells in areas of the heart most receptive to stem cell therapy and monitor the efficacy of treatment by reporting the viability, location, and number of implanted stem cells. We describe a multimodal, silica-based nanoparticle that can be used for cell sorting (fluorescence), real-time guided cell implantation ultrasound, and high-resolution, long-term monitoring by magnetic resonance imaging (MRI)...
March 20, 2013: Science Translational Medicine
Yuyu Yao, Zulong Sheng, YeFei Li, Cong Fu, Genshan Ma, Naifeng Liu, Julie Chao, Lee Chao
Endothelial progenitor cells (EPCs) have been shown to enhance angiogenesis not only by incorporating into the vasculature but also by secreting cytokines, thereby serving as an ideal vehicle for gene transfer. As tissue kallikrein (TK) has pleiotropic effects in inhibiting apoptosis and oxidative stress, and promoting angiogenesis, we evaluated the salutary potential of kallikrein-modified human EPCs (hEPCs; Ad.hTK-hEPCs) after acute myocardial infarction (MI). We genetically modified hEPCs with a TK gene and evaluated cell survival, engraftment, revascularization, and functional improvement in a nude mouse left anterior descending ligation model...
May 2013: Laboratory Investigation; a Journal of Technical Methods and Pathology
Salvatore De Rosa, Florian H Seeger, Jörg Honold, Ulrich Fischer-Rasokat, Ralf Lehmann, Stephan Fichtlscherer, Volker Schächinger, Stefanie Dimmeler, Andreas M Zeiher, Birgit Assmus
BACKGROUND: Cell-based therapies are a promising option in patients with acute myocardial infarction or chronic heart failure (CHF). However, administration of cells requires intracoronary or intracardiac instrumentation, which is potentially associated with periprocedural risks. Therefore, we analyzed periprocedural complications and 30-day outcome in 775 consecutive procedures of intracoronary administration of progenitor cells using the stop-flow technique. METHODS AND RESULTS: Indications for cell administration were acute myocardial infarction (n=126) and CHF of ischemic (n=562) or nonischemic (n=87) etiology...
February 2013: Circulation. Cardiovascular Interventions
Naosumi Sekiya, Kimimasa Tobita, Sarah Beckman, Masaho Okada, Burhan Gharaibeh, Yoshiki Sawa, Robert L Kormos, Johnny Huard
Direct intracardiac cell injection for heart repair is hindered by numerous limitations including: cell death, poor spreading of the injected cells, arrhythmia, needle injury, etc. Tissue-engineered cell sheet implantation has the potential to overcome some of these limitations. We evaluated whether the transplantation of a muscle-derived stem cell (MDSC) sheet could improve the regenerative capacity of MDSCs in a chronic model of myocardial infarction. MDSC sheet-implanted mice displayed a reduction in left ventricle (LV) dilation and sustained LV contraction compared with the other groups...
March 2013: Molecular Therapy: the Journal of the American Society of Gene Therapy
Yunshan Zhao, Tieluo Li, Xufeng Wei, Giacomo Bianchi, Jingping Hu, Pablo G Sanchez, Kai Xu, Pei Zhang, Mark F Pittenger, Zhongjun J Wu, Bartley P Griffith
Progressive cardiac remodeling, including the myopathic process in the adjacent zone following myocardial infarction (MI), contributes greatly to the development of cardiac failure. Cardiomyoplasty using bone marrow-derived mesenchymal stem cells (MSCs) has been demonstrated to protect cardiomyocytes and/or repair damaged myocardium, leading to improved cardiac performance, but the therapeutic effects on cardiac remodeling are still under investigation. Here, we tested the hypothesis that MSCs could improve the pathological remodeling of the adjacent myocardium abutting the infarct...
September 2012: Stem Cells Translational Medicine
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