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Intracardiac cell transplantation

Xingui Peng, Cong Li, Yingying Bai, Xinyi Wang, Yi Zhang, Yanli An, Gao-Jun Teng, Shenghong Ju
Background: Endothelial progenitor cells (EPCs) play an important role in repairing ischemia tissues. However, the survival, migration and therapeutic efficacy of EPCs after transplantation need to be better understood for further cell therapy. Purpose: This study investigated the migration effect of EPCs labeled with a multimodal imaging agent in a murine ischemic hindlimb model, using magnetic resonance imaging (MRI) and optical imaging after transplantation. Methods: EPCs derived from mouse bone marrow were labeled with a multimodal imaging agent and were administered through intracardiac delivery to mice with ischemic hindlimbs...
2018: International Journal of Nanomedicine
María J Contreras-Zárate, D Ryan Ormond, Austin E Gillen, Colton Hanna, Nicole L Day, Natalie J Serkova, Britta M Jacobsen, Susan M Edgerton, Ann D Thor, Virginia F Borges, Kevin O Lillehei, Michael W Graner, Peter Kabos, Diana M Cittelly
Brain metastases are an increasing burden among breast cancer patients, particularly for those with HER2+ and triple negative (TN) subtypes. Mechanistic insight into the pathophysiology of brain metastases and preclinical validation of therapies has relied almost exclusively on intracardiac injection of brain-homing cells derived from highly aggressive TN MDA-MB-231 and HER2+ BT474 breast cancer cell lines. Yet, these well characterized models are far from representing the tumor heterogeneity observed clinically and, due to their fast progression in vivo , their suitability to validate therapies for established brain metastasis remains limited...
2017: Frontiers in Oncology
Martin Maldonado, Tianhua Huang, Lujun Yang, Lan Xu, Lian Ma
BACKGROUND: We evaluated the therapeutic effect and fate of high doses of human umbilical cord Wharton jelly cells (hUCWJCs) after IP administration to streptozotocin (STZ)-induced diabetic mice. METHODS: Type 1 diabetes (T1D) was induced in Kunming mice via IP injection of STZ. hUCWJCs were labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI). Diabetic animals with sustained hyperglycemia for at least 2 weeks were administered 1 × 107 Dil-hUCWJCs via intraperitoneal injection...
October 17, 2017: Cell Communication and Signaling: CCS
Nikoletta Sztán, Bence Lázár, Nóra Bodzsár, Barbara Végi, Krisztina Liptói, Bertrand Pain, Eszter Patakiné Várkonyi
The conservation of genetic resources of avian species has become increasingly important over the past decade. The aim of the present study was to develop a genome preservation technique for the Hungarian goose Anser anser domestica. To this end, we developed a novel approach combining the simplicity of isolating a blastodermal cell suspension, which includes forming primordial germ cells (PGCs), with the efficiency of targeting future gonads by injecting these cells into the cardiac vein of the developing host embryo...
March 27, 2017: Reproduction, Fertility, and Development
Tiam Feridooni, Adam Hotchkiss, Mark Baguma-Nibasheka, Feixiong Zhang, Brittney Allen, Sarita Chinni, Kishore B S Pasumarthi
β-Adrenergic receptors (β-ARs) and catecholamines are present in rodents as early as embryonic day (E)10.5. However, it is not known whether β-AR signaling plays any role in the proliferation and differentiation of ventricular cells in the embryonic heart. Here, we characterized expression profiles of β-AR subtypes and established dose-response curves for the nonselective β-AR agonist isoproterenol (ISO) in the developing mouse ventricular cells. Furthermore, we investigated the effects of ISO on cell cycle activity and differentiation of cultured E11...
May 1, 2017: American Journal of Physiology. Heart and Circulatory Physiology
Juan Wang, Guang-Liang Chen, Shan Cao, Ming-Chun Zhao, Yong-Qing Liu, Xiao-Xiang Chen, Cheng Qian
Bone marrow (BM) adipocytes are abundant in BM and may be involved in the process of bone metastasis. However, their behaviors in metastatic BM niches during bone metastasis have not been fully explored. In this study, intracardiac transplantation of B16-F10 melanoma cells into immunocompetent C57BL/6 mice was performed. Tibial marrow sections were stained with hematoxylin and eosin, Masson's trichrome, tartrate-resistant acid phosphatase, and fatty acid-binding protein 4 (FABP4) and analyzed using a histomorphometric system...
June 2017: Laboratory Investigation; a Journal of Technical Methods and Pathology
Geoffrey Burnstock
There is nervous control of the heart by ATP as a cotransmitter in sympathetic, parasympathetic, and sensory-motor nerves, as well as in intracardiac neurons. Centers in the brain control heart activities and vagal cardiovascular reflexes involve purines. Adenine nucleotides and nucleosides act on purinoceptors on cardiomyocytes, AV and SA nodes, cardiac fibroblasts, and coronary blood vessels. Vascular tone is controlled by a dual mechanism. ATP, released from perivascular sympathetic nerves, causes vasoconstriction largely via P2X1 receptors...
January 6, 2017: Circulation Research
Muhammad Jamal Afridi, Arun Ross, Xiaoming Liu, Margaret F Bennewitz, Dorela D Shuboni, Erik M Shapiro
PURPOSE: Magnetic resonance imaging (MRI)-based cell tracking has emerged as a useful tool for identifying the location of transplanted cells, and even their migration. Magnetically labeled cells appear as dark contrast in T2*-weighted MRI, with sensitivities of individual cells. One key hurdle to the widespread use of MRI-based cell tracking is the inability to determine the number of transplanted cells based on this contrast feature. In the case of single cell detection, manual enumeration of spots in three-dimensional (3D) MRI in principle is possible; however, it is a tedious and time-consuming task that is prone to subjectivity and inaccuracy on a large scale...
November 2017: Magnetic Resonance in Medicine: Official Journal of the Society of Magnetic Resonance in Medicine
Rui Wei, Jin Yang, Meijuan Gao, Haining Wang, Wenfang Hou, Yiming Mu, Guian Chen, Tianpei Hong
Microenvironment regulates cell fate and function. In this study, we investigated the effects of the infarcted cardiac microenvironment on cardiac differentiation of human embryonic stem cells (hESCs). hESCs were intramyocardially transplanted into infarcted or uninjured rat hearts. After 4 weeks, mesodermal and cardiac lineage markers were detected by immunofluorescence. Cardiac function was assessed by echocardiography. hESCs were differentiated in vitro under hypoxic (5% O2 ), low-nutrient (5% FBS), or control condition...
November 2016: Cell Biology International
Natesh Parashurama, Byeong-Cheol Ahn, Keren Ziv, Ken Ito, Ramasamy Paulmurugan, Jürgen K Willmann, Jaehoon Chung, Fumiaki Ikeno, Julia C Swanson, Denis R Merk, Jennifer K Lyons, David Yerushalmi, Tomohiko Teramoto, Hisanori Kosuge, Catherine N Dao, Pritha Ray, Manishkumar Patel, Ya-Fang Chang, Morteza Mahmoudi, Jeff Eric Cohen, Andrew Brooks Goldstone, Frezghi Habte, Srabani Bhaumik, Shahriar Yaghoubi, Robert C Robbins, Rajesh Dash, Phillip C Yang, Todd J Brinton, Paul G Yock, Michael V McConnell, Sanjiv S Gambhir
Purpose To quantitatively determine the limit of detection of marrow stromal cells (MSC) after cardiac cell therapy (CCT) in swine by using clinical positron emission tomography (PET) reporter gene imaging and magnetic resonance (MR) imaging with cell prelabeling. Materials and Methods Animal studies were approved by the institutional administrative panel on laboratory animal care. Seven swine received 23 intracardiac cell injections that contained control MSC and cell mixtures of MSC expressing a multimodality triple fusion (TF) reporter gene (MSC-TF) and bearing superparamagnetic iron oxide nanoparticles (NP) (MSC-TF-NP) or NP alone...
September 2016: Radiology
Wen-Chi Yang, Ming Yao, Yu-Hsuan Chen, Sung-Hsin Kuo
We present a rare case of intracardiac myeloid sarcoma (MS) of acute myeloid leukemia (AML) and who responds completely well to low-dose radiotherapy. This 19-year-old young man initially presented with AML and received standard chemotherapy followed by allogeneic hematopoietic stem cell transplantation (HSCT). However, he developed intracardiac isolated MS relapse with the presentation of exertional dyspnea and superior vena cava (SVC) syndrome 3 years later. He then received radiotherapy with 24 Gy at a 12 daily fractions using forward "field in field" intensity modulated radiotherapy technique...
June 2016: Journal of Thoracic Disease
Karin Boer, Kadir Caliskan, Annemiek M A Peeters, Marian C van Groningen, Janneke N Samsom, Alexander P W M Maat, Michiel G H Betjes, Willem Weimar, Carla C Baan
BACKGROUND: FOXP3+ regulatory T cells (Treg) either originate in the thymus (natural [n]Treg) or are induced in the periphery by antigen exposure and cytokines (induced [i]Treg). It is currently not elucidated which and to what extent these Treg subsets regulate intracardiac allogeneic responses in transplant patients. METHODS: By using demethylation of the Treg-specific demethylated region in the FOXP3 gene as a marker for nTreg and FOXP3 messenger RNA expression as a marker for the total Treg population, we examined Treg in endomyocardial biopsies (EMBs) of both patients who developed an acute rejection necessitating therapy (rejectors; International Society for Heart and Lung Transplantation rejection grade ≥ 2R) and patients who remained free from rejection (nonrejectors)...
September 2015: Transplantation
Prabhjot Singh Nijjar, Sofia Carolina Masri, Ashenafi Tamene, Helina Kassahun, Kenneth Liao, Uma Valeti
Primary cardiac tumors are far rarer than tumors metastatic to the heart. Angiosarcoma is the primary cardiac neoplasm most frequently detected; lymphomas constitute only 1% of primary cardiac tumors. We present the case of a 55-year-old woman with a recently diagnosed intracardiac mass who was referred to our institution for consideration of urgent orthotopic heart transplantation. Initial images suggested an angiosarcoma; however, a biopsy specimen of the mass was diagnostic for diffuse large B-cell lymphoma...
December 2014: Texas Heart Institute Journal
Paula H Suss, Luiz Guilherme A Capriglione, Fabiane Barchiki, Lye Miyague, Danielle Jackowski, Letícia Fracaro, Andressa V Schittini, Alexandra C Senegaglia, Carmen L K Rebelatto, Márcia Olandoski, Alejandro Correa, Paulo R S Brofman
The development of new therapeutic strategies is necessary to reduce the worldwide social and economic impact of cardiovascular disease, which produces high rates of morbidity and mortality. A therapeutic option that has emerged in the last decade is cell therapy. The aim of this study was to compare the effect of transplanting human umbilical cord-derived stromal cells (UCSCs), human umbilical cord blood-derived endothelial cells (UCBECs) or a combination of these two cell types for the treatment of ischemic cardiomyopathy (IC) in a Wistar rat model...
July 2015: Experimental Biology and Medicine
Fang-Yuan Wei, Kwok-Sui Leung, Gang Li, Jianghui Qin, Simon Kwoon-Ho Chow, Shuo Huang, Ming-Hui Sun, Ling Qin, Wing-Hoi Cheung
Low intensity pulsed ultrasound (LIPUS) has been proven effective in promoting fracture healing but the underlying mechanisms are not fully depicted. We examined the effect of LIPUS on the recruitment of mesenchymal stem cells (MSCs) and the pivotal role of stromal cell-derived factor-1/C-X-C chemokine receptor type 4 (SDF-1/CXCR4) pathway in response to LIPUS stimulation, which are essential factors in bone fracture healing. For in vitro study, isolated rat MSCs were divided into control or LIPUS group. LIPUS treatment was given 20 minutes/day at 37 °C for 3 days...
2014: PloS One
Hv New, Ce Chapman, Md Kilby, S Kumar, Cj Brown, Phb Bolton-Maggs
: For intrauterine transfusion (IUT) specific red cells are provided by UK Blood Services, irradiated to prevent TA-GvHD. A fetus (21/40) was referred to a fetal medicine centre (FMC), severely anaemic (parvovirus) requiring urgent IUT. Maternal blood was transfused to fetus. Following poor cardiac output, further emergency intracardiac transfusion gave improvement. At 32/40 baby was delivered, severely hydropic and pancytopenic (aplasia later confirmed by marrow aspirate). Hyperbilirubinaemia and fungal chest infection followed...
June 2014: Archives of Disease in Childhood. Fetal and Neonatal Edition
Jesse D Vrecenak, Erik G Pearson, Matthew T Santore, Carlyn A Todorow, Haiying Li, Antoneta Radu, Tricia Bhatti, William H Peranteau, Mark P Johnson, Alan W Flake
Evidence supporting the efficacy of in utero hematopoietic cell transplantation (IUHCT) in a valid large animal model is needed prior to clinical application. The objective of this study was to establish clinically relevant levels of hematopoietic chimerism in a canine model of maternal-to-fetal IUHCT. We first assessed immune and hematopoietic ontogeny relevant to IUHCT in the canine model and identified 40 days' gestation (term 63 days) as a time point at the initiation of thymic selection, and prior to bone marrow hematopoiesis, that might be optimal for IUHCT...
September 18, 2014: Blood
Andreas Martens, Sebastian V Rojas, Hassina Baraki, Christian Rathert, Natalie Schecker, Robert Zweigerdt, Kristin Schwanke, Sara Rojas-Hernandez, Ulrich Martin, Shunsuke Saito, Jan D Schmitto, Axel Haverich, Ingo Kutschka
The limited success of cardiac stem cell therapy has lately generated discussion regarding its effectiveness. We hypothesized that immediate cell loss after intramyocardial injection significantly obscures the regenerative potential of stem cell therapy. Therefore, our aim was to assess the distribution and quantity of induced pluripotent stem cells after intramyocardial delivery using in vivo bioluminescence analysis. In this context, we wanted to investigate if the injection of different cell concentrations would exert influence on cardiac cell retention...
November 2014: Artificial Organs
Yuji Shiba, Dominic Filice, Sarah Fernandes, Elina Minami, Sarah K Dupras, Benjamin Van Biber, Peter Trinh, Yusuke Hirota, Joseph D Gold, Mohan Viswanathan, Michael A Laflamme
BACKGROUND: Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) were recently shown to be capable of electromechanical integration following direct injection into intact or recently injured guinea pig hearts, and hESC-CM transplantation in recently injured hearts correlated with improvements in contractile function and a reduction in the incidence of arrhythmias. The present study was aimed at determining the ability of hESC-CMs to integrate and modulate electrical stability following transplantation in a chronic model of cardiac injury...
July 2014: Journal of Cardiovascular Pharmacology and Therapeutics
Anne-Marie Bleau, Javier Freire, María José Pajares, Isabel Zudaire, Iker Anton, Estanislao Nistal-Villán, Miriam Redrado, Caroli Na Zandueta, Irati Garmendia, Daniel Ajona, David Blanco, Ruben Pio, Fernando Lecanda, Alfonso Calvo, Luis M Montuenga
New mouse models with specific drivers of genetic alterations are needed for preclinical studies. Herein, we created and characterized at the genetic level a new syngeneic model for lung cancer and metastasis in Balb-c mice. Tumor cell lines were obtained from a silica-mediated airway chronic inflammation that promotes tumorigenesis when combined with low doses of N-nitrosodimethylamine, a tobacco smoke carcinogen. Orthotopic transplantation of these cells induced lung adenocarcinomas, and their intracardiac injection led to prominent colonization of various organs (bone, lung, liver and brain)...
December 1, 2014: International Journal of Cancer. Journal International du Cancer
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