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https://www.readbyqxmd.com/read/28531267/alignment-of-1000-genomes-project-reads-to-reference-assembly-grch38
#1
Xiangqun Zheng-Bradley, Ian Streeter, Susan Fairley, David Richardson, Laura Clarke, Paul Flicek
BACKGROUND: The 1000 Genomes Project produced more than 100 trillion basepairs of short read sequence from more than 2600 samples in 26 populations over a period of five years. In its final phase, the project released over 85 million genotyped and phased variants on human reference genome assembly GRCh37. An updated reference assembly, GRCh38, was released in late 2013, but there was insufficient time for the final phase of the project analysis to change to the new assembly. Although it is possible to lift the coordinates of the 1000 Genomes project variants to the new assembly, this is a potentially error prone process as coordinate remapping is most appropriate only for non-repetitive regions of the genome and those that did not see significant change between the two assemblies...
May 20, 2017: GigaScience
https://www.readbyqxmd.com/read/28531108/regulation-of-metabolic-activity-by-p53
#2
REVIEW
Jessica Flöter, Irem Kaymak, Almut Schulze
Metabolic reprogramming in cancer cells is controlled by the activation of multiple oncogenic signalling pathways in order to promote macromolecule biosynthesis during rapid proliferation. Cancer cells also need to adapt their metabolism to survive and multiply under the metabolically compromised conditions provided by the tumour microenvironment. The tumour suppressor p53 interacts with the metabolic network at multiple nodes, mostly to reduce anabolic metabolism and promote preservation of cellular energy under conditions of nutrient restriction...
May 20, 2017: Metabolites
https://www.readbyqxmd.com/read/28530619/-current-status-of-laparoscopic-sacrocolpopexy-in-the-treatment-of-pelvic-organ-prolapse
#3
Carmen González-Enguita, Natalia Gennaro-DellaRossa, Esther López-López, Juliana Escobar-Castaño, Percy Miguel Rodríguez-Castro, Raquel González-López
OBJECTIVES: Surgical restoration of pelvic floor anatomy in pelvic organ prolapse (POP) should avoid extensive areas that may injure healthy tissues and lead to scar fibrosis producing dysfunctional rigidity. Laparoscopic sacrocolpopexy corrects POP by lifting pelvic elements with a minimally invasive procedure.Various current strategies and approaches make it a diverse procedure. METHODS: We performed a bibliographic review on the published experience about abdominal sacrocolpopexy over the last 20 years...
May 2017: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/28530130/injectable-graft-substitute-active-on-bone-tissue-regeneration
#4
Michela Bosetti, Alessia Borrone, Massimiliano Leigheb, Prasad Shastri, Mario Cannas
With the aim to obtain an injectable bioactive scaffold that can accelerate bone formation in sinus lift augmentation, in bony void and in fracture repair, we have developed a three-dimensional (3D) jelly collagen containing Lysophosphatidic acid (LPA) and 1α,25-Dihydroxyvitamin D3 (1,25D3). Using an in vitro 3D culture model of bone fracture we show that the contraction of the collagen gel is mediated by Rho-kinase activation in osteoblasts. This contraction was cell concentration dependent and was increased by LPA which favored apposition and fastening of bone fragments approach...
May 20, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28529384/the-pelskin-project-part-v-towards-the-control-of-the-flow-around-aerofoils-at-high-angle-of-attack-using-a-self-activated-deployable-flap
#5
Marco E Rosti, Laura Kamps, Christoph Bruecker, Mohammad Omidyeganeh, Alfredo Pinelli
During the flight of birds, it is often possible to notice that some of the primaries and covert feathers on the upper side of the wing pop-up under critical flight conditions, such as the landing approach or when stalking their prey (see Fig. 1) . It is often conjectured that the feathers pop up plays an aerodynamic role by limiting the spread of flow separation . A combined experimental and numerical study was conducted to shed some light on the physical mechanism determining the feathers self actuation and their effective role in controlling the flow field in nominally stalled conditions...
2017: Meccanica
https://www.readbyqxmd.com/read/28528368/preparation-of-allogeneic-bone-for-alveolar-ridge-augmentation
#6
Kornel Krasny, Artur Kamiński, Marta Krasny, Tomasz Czech, Andrzej Wojtowicz
Implant treatment is safe and predictable with sufficient amount and quality of bone tissue. In case of severely reduced bone tissue after a tooth was lost, augmentation of such tissue is necessary before implant embedment. Retrospective evaluation covered 380 alveolar ridge reconstructions. The study material consisted of human grafts prepared by the Department of Transplantology and Central Tissue Bank, Medical University of Warsaw. Presentation of laboratory procedures in the context of physical parameters of frozen, radiation sterilised, allogeneic corticocancellous material was presented...
May 20, 2017: Cell and Tissue Banking
https://www.readbyqxmd.com/read/28528237/skin-deformation-during-shoulder-movements-and-upper-extremity-activities
#7
Weiqing Ge, Alison Sfara, Brittany Hians
BACKGROUND: The necessity of sternal precautions for patients following cardiac surgery with median sternotomy has been questioned by clinicians, researchers, and even patients. The primary purpose was to determine if sternal skin deformation during shoulder movements and upper extremity activities is compressive or distractive and if there are any significant differences between the skin deformation at different positions during shoulder movements and upper extremity activities. The secondary purpose was to determine if sternal skin deformation is correlated with scapular stabilizer muscle strength...
May 12, 2017: Clinical Biomechanics
https://www.readbyqxmd.com/read/28527583/molecular-characterization-of-aspergillus-fumigatus-tcsc-a-characteristic-type-iii-hybrid-histidine-kinase-of-filamentous-fungi-harboring-six-hamp-domains
#8
Anja Spadinger, Frank Ebel
The type III hybrid histidine kinase (HHK) TcsC enables the pathogenic mold Aspergillus fumigatus to thrive under hyperosmotic conditions. It is, moreover, of particular interest, since it is the target of certain antifungal agents, such as fludioxonil. This study was aimed at a functional characterization of the domains that constitute the sensing and the kinase module of TcsC. The sensing module consists of six HAMP domains, an architecture that is commonly found in type III HHKs of filamentous fungi. To dissect the functional role of the individual domains, we have analyzed a set of truncated derivatives of TcsC with respect to their impact on fungal growth and their ability to respond to hyperosmotic stress and fludioxonil...
May 11, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28526513/a-leadership-intervention-to-further-the-training-of-female-faculty-lift-off-in-radiology
#9
Lucy B Spalluto, Stephanie E Spottswood, Lori A Deitte, Alexander Chern, Charlene M Dewey
RATIONALE AND OBJECTIVES: Women are under-represented in the field of radiology, occupy a minority of leadership positions, and, at our institution, have not achieved the same level of academic success as their male counterparts. Consequently, the authors designed, implemented, and evaluated the Leadership Intervention to Further the Training of Female Faculty (LIFT-OFF) program to (1) improve access to opportunities for women's faculty development and advancement, and (2) improve clarification of expectations about the role and path of advancement...
March 24, 2017: Academic Radiology
https://www.readbyqxmd.com/read/28526082/proof-of-concept-non-invasive-estimation-of-peripheral-venous-oxygen-saturation
#10
Musabbir Khan, Chris G Pretty, Alexander C Amies, Joel Balmer, Houda E Banna, Geoffrey M Shaw, J Geoffrey Chase
BACKGROUND: Pulse oximeters continuously monitor arterial oxygen saturation. Continuous monitoring of venous oxygen saturation (SvO2) would enable real-time assessment of tissue oxygen extraction (O2E) and perfusion changes leading to improved diagnosis of clinical conditions, such as sepsis. METHODS: This study presents the proof of concept of a novel pulse oximeter method that utilises the compliance difference between arteries and veins to induce artificial respiration-like modulations to the peripheral vasculature...
May 19, 2017: Biomedical Engineering Online
https://www.readbyqxmd.com/read/28522914/one-year-experience-with-computer-assisted-propofol-sedation-for-colonoscopy
#11
Otto S Lin, Danielle La Selva, Richard A Kozarek, Deborah Tombs, Wade Weigel, Ryan Beecher, Johannes Koch, Susan McCormick, Michael Chiorean, Fred Drennan, Michael Gluck, Nanda Venu, Michael Larsen, Andrew Ross
AIM: To report our one-year experience with computer assisted propofol sedation (CAPS) for colonoscopy as the first United States Medical Center to adopt CAPS technology for routine clinical use. METHODS: Between September 2014 and August 2015, 2677 patients underwent elective outpatient colonoscopy with CAPS at our center. All colonoscopies were performed by 1 of 17 gastroenterologists certified in the use of the CAPS system, with the assistance of a specially trained nurse...
April 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28522246/patient-obesity-influences-pelvic-lift-during-cup-insertion-in-total-hip-arthroplasty-through-a-lateral-transgluteal-approach-in-supine-position
#12
Steffen Brodt, Dimitri Nowack, Benjamin Jacob, Linda Krakow, Christoph Windisch, Georg Matziolis
BACKGROUND: Movement of the pelvis during implantation of total hip arthroplasty (THA) has a major influence on the positioning of the acetabular cup. Strong traction caused by retractors leads to iatrogenic pelvic lift and can thus be partly responsible for cup malpositioning. The objective of this study was to investigate such factors that influence pelvic lift. METHODS: The dynamic movement of the pelvis was measured during implantation of THA in 67 patients...
April 21, 2017: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28520629/blepharoplasty-effect-on-a-described-algorithmic-approach-to-external-ptosis-repair-is-it-time-for-unbundling
#13
Tal J Rubinstein, Daniel J Repp, Bryan S Sires
PURPOSE: To investigate the effects of performing a previously described algorithmic levator resection for involutional ptosis with a blepharoplasty instead of through a small incision. METHODS: Eyelids with involutional ptosis and normal levator function were included in the study. An upper blepharoplasty was performed first. An external levator resection was then performed based on a described technique involving 2 mm resection of aponeurosis for 1 mm of desired lift, consistent tension on the aponeurosis between surgical cases, and standardized suture placement...
May 17, 2017: Ophthalmic Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28516295/judgments-of-differences-and-ratios-of-subjective-heaviness
#14
Sergio Cesare Masin, Andrea Brancaccio
Experimental instructions to judge differences or ratios of subjective heaviness numerically are generally assumed to produce judgments linearly proportional to the respective heaviness differences or heaviness ratios. In this study, participants were instructed to numerically judge the difference or ratio of heaviness between two weights being lifted separately, either unimanually or bimanually. Weight values were combined factorially. Patterns of factorial curves revealed that unimanual lifting triggered linear judgments of heaviness differences, whereas bimanual lifting triggered nonlinear judgments of heaviness ratios...
May 17, 2017: Attention, Perception & Psychophysics
https://www.readbyqxmd.com/read/28515839/hypotensive-responses-of-reciprocal-supersets-versus-traditional-resistance-training-in-apparently-healthy-men
#15
Claudio M Bentes, Pablo B Costa, Victor G Corrêa Neto, Roberto Simão, Gabriel A Paz, Marianna F Maia, Tiago Figueiredo, Gabriel R Neto, Jefferson S Novaes, Humberto Miranda
The purpose of this study was to compare the hypotensive responses of reciprocal supersets (SS) versus traditional training (TRAD) methods. Thirteen men with at least five years of recreational experience in resistance training (RT) volunteered for the study. When completing the TRAD protocol, participants performed the following exercises separately in sequence: chest press (CP), low row (LR), leg extension (LE), leg curl (LC), pull down (PD), and shoulder press (SP). The SS method required participants to complete the same exercises as in the TRAD protocol, but exercises were coupled such that muscles sequentially served both as an agonist for lift one and then antagonist for lift two and vice versa...
2017: International Journal of Exercise Science
https://www.readbyqxmd.com/read/28515837/resistance-training-contribute-to-the-aerobic-components-of-an-exercise-session-in-adults-but-not-as-much-in-older-adults
#16
Neha Bharti, Travis Hrubeniuk, Andrea Mayo, Martin Sénéchal, Danielle R Bouchard
Previous research has indicated that active adults are able to achieve moderate intensity as measured via heart rate during a typical resistance training (RT) session. The main objective was to identify if overweight adults and older adults can reach aerobic moderate intensity at a rate comparable to adults displaying a recommended body mass index. Twenty participants in each group were asked to visit a fitness facility twice if they self-reported doing RT for a minimum two days per week. At the first session baseline characteristics and maximal lifting capacity for each RT exercise...
2017: International Journal of Exercise Science
https://www.readbyqxmd.com/read/28513472/experimental-and-numerical-studies-of-beetle-inspired-flapping-wing-in-hovering-flight
#17
Tien Van Truong, Tuyen Quang Le, Hoon Cheol Park, Doyoung Byun
In this paper, we measure unsteady forces and visualize 3D vortices around a beetle-like flapping wing model in hovering flight by experiment and numerical simulation. The measurement of unsteady forces and flow patterns around the wing were conducted using a dynamically scaled wing model in the mineral-oil tank. The wing kinematics were directly derived from the experiment of a real beetle. The 3D flow structures of the flapping wing were captured by using air bubble visualization while forces were measured by a sensor attached at the wing base...
May 17, 2017: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/28512698/repair-of-the-pronator-quadratus-after-volar-plate-fixation-in-distal-radius-fractures-a-systematic-review
#18
REVIEW
Marjolein A M Mulders, Monique M J Walenkamp, Fernande J M E Bos, Niels W L Schep, J Carel Goslings
To position the volar plate on the distal radius fracture site, the pronator quadratus muscle needs to be detached from its distal and radial side and lifted for optimal exposure to the fracture site. Although the conventional approach involves repair of the pronator quadratus, controversy surrounds the merits of this repair. The purpose of this study was to compare the functional outcomes of patients with distal radius fractures treated with pronator quadratus repair after volar plate fixation versus no pronator quadratus repair...
May 17, 2017: Strategies in Trauma and Limb Reconstruction
https://www.readbyqxmd.com/read/28510538/measuring-and-assessing-individual-external-doses-during-the-rehabilitation-phase-in-iitate-village-after-the-fukushima-daiichi-nuclear-power-plant-accident
#19
Wataru Naito, Motoki Uesaka, Tadahiro Kurosawa, Yujiro Kuroda
After the Fukushima Daiichi Nuclear Power Plant accident in 2011, Iitate village was placed under an evacuation order because the level of radioactive materials drifting from the nuclear plant to the village were above a government-set level for allowing residents to live in the area. The evacuation advisory for most of the village was lifted on March 31, 2017. For displaced residents deciding whether or not to return to their homes, it is important to correctly understand and estimate the realistic individual external doses they will receive after returning to the village...
May 16, 2017: Journal of Radiological Protection: Official Journal of the Society for Radiological Protection
https://www.readbyqxmd.com/read/28508761/posterior-branches-of-lumbar-spinal-nerves-part-ii-lumbar-facet-syndrome-pathomechanism-symptomatology-and-diagnostic-work-up
#20
Katarzyna Kozera, Bogdan Ciszek, Paweł Szaro
Posterior branches of the lumbar spinal nerves are the anatomic substrate of pain in the lower back, sacrum and the gluteal area. Such pain may be associated with various pathologies which cause pain in the posterior branches of the lumbar spinal nerves due to entrapment, mechanical irritation or inflammatory reaction and/or degeneration. The posterior branches are of significant functional importance, which is related to the function of the structures they supply, including facet joints, which are the basic biomechanical units of the spine...
April 12, 2017: Ortopedia, Traumatologia, Rehabilitacja
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