Read by QxMD icon Read

Ultra MIS

Vladyslav S Bondarenko, Mikhail S Gelfand
A typical eukaryotic gene is comprised of alternating stretches of regions, exons and introns, retained in and spliced out a mature mRNA, respectively. Although the length of introns may vary substantially among organisms, a large fraction of genes contains short introns in many species. Notably, some Ciliates (Paramecium and Nyctotherus) possess only ultra-short introns, around 25 bp long. In Paramecium, ultra-short introns with length divisible by three (3n) are under strong evolutionary pressure and have a high frequency of in-frame stop codons, which, in the case of intron retention, cause premature termination of mRNA translation and consequent degradation of the mis-spliced mRNA by the nonsense-mediated decay mechanism...
2016: PloS One
Timothy Y Wang, S Harrison Farber, Scott S Perkins, Adam G Back, Sarah A Byrd, Debbie Chi, David Vincent, Isaac O Karikari
STUDY DESIGN: Randomized controlled trial OBJECTIVE.: Compare radiation exposure between ultra-low radiation imaging (ULRI) with image enhancement and standard-dose fluoroscopy for patients undergoing minimally invasive transforaminal lumbar interbody fusion (MIS TLIF) SUMMARY OF BACKGROUND DATA.: While the benefits of MIS are lauded by many, there is a significant amount of radiation exposure to surgeon and operating room (OR) personnel. Our goal with this work was to see if by using ultra-low dose radiation settings coupled with image enhancement, this exposure could be minimized...
May 31, 2016: Spine
Anders Ståhlberg, Paul M Krzyzanowski, Jennifer B Jackson, Matthew Egyud, Lincoln Stein, Tony E Godfrey
Detection of cell-free DNA in liquid biopsies offers great potential for use in non-invasive prenatal testing and as a cancer biomarker. Fetal and tumor DNA fractions however can be extremely low in these samples and ultra-sensitive methods are required for their detection. Here, we report an extremely simple and fast method for introduction of barcodes into DNA libraries made from 5 ng of DNA. Barcoded adapter primers are designed with an oligonucleotide hairpin structure to protect the molecular barcodes during the first rounds of polymerase chain reaction (PCR) and prevent them from participating in mis-priming events...
June 20, 2016: Nucleic Acids Research
Hyun Jeong, Hye Min Oh, Seungho Bang, Hyeon Jun Jeong, Sung-Jin An, Gang Hee Han, Hyun Kim, Seok Joon Yun, Ki Kang Kim, Jin Cheol Park, Young Hee Lee, Gilles Lerondel, Mun Seok Jeong
We present a novel metal-insulator-semiconductor (MIS) diode consisting of graphene, hexagonal BN, and monolayer MoS2 for application in ultrathin nanoelectronics. The MIS heterojunction structure was fabricated by vertically stacking layered materials using a simple wet chemical transfer method. The stacking of each layer was confirmed by confocal scanning Raman spectroscopy and device performance was evaluated using current versus voltage (I-V) and photocurrent measurements. We clearly observed better current rectification and much higher current flow in the MIS diode than in the p-n junction and the metal-semiconductor diodes made of layered materials...
March 9, 2016: Nano Letters
D Batouche, K Elhalimi, S Abassini, N Benatta, H Messali, N Chaib, M Negadi, Z Mentouri
OBJECTIFS: L'objectif est de fournir les informations spécifiques d'un orage adrénergique lors d'un stress violent chez l'enfant. MéTHODES: 2 observations cliniques chez des enfants ayant subi un traumatisme violent. RéSULTATS: 1(er) cas : CA 12 ans, admis en réanimation pédiatrique pour une noyade stade3, le 2 août 2015. Il a été intubé, sédaté ventilé en auto-contrôle, avec une peep à 7. Une administration de la noradrénaline à 0,2 gamma/kg/min pour optimiser les pressions artérielles moyennes (PAM) qui restaient stables aux alentours de 88mmHg...
December 2015: Annales de Cardiologie et D'angéiologie
Tim Jäger, Claudia Scherr, Devika Shah, Vera Majewsky, Ursula Wolf, Lucietta Betti, Stephan Baumgartner
OBJECTIVES: The objective was to evaluate homeopathic basic research studies that use plant-based bioassays. With this in view, a compilation was made of the findings of three systematic literature reviews covering plant-based bioassays in the three fields of healthy, abiotically, or biotically stressed plants. This compilation focused on investigations using advanced experimental methods and detailed descriptions, also with the aim of supporting the design of future experiments. METHODS: Publications included had to report on studies into the effects of homeopathic preparations on whole plants, seeds, plant parts and cells...
October 2015: Homeopathy: the Journal of the Faculty of Homeopathy
Sarah A Sabatinos, Nimna S Ranatunga, Ji-Ping Yuan, Marc D Green, Susan L Forsburg
DNA replication stress causes genome mutations, rearrangements, and chromosome missegregation, which are implicated in cancer. We analyze a fission yeast mutant that is unable to complete S phase due to a defective subunit of the MCM helicase. Despite underreplicated and damaged DNA, these cells evade the G2 damage checkpoint to form ultrafine bridges, fragmented centromeres, and uneven chromosome segregations that resembles micronuclei. These micronuclei retain DNA damage markers and frequently rejoin with the parent nucleus...
October 1, 2015: Molecular Biology of the Cell
Jerzy Garcarek, Jacek Kurcz, Maciej Guziński, Mirosław Banasik, Marcin Miś, Tomasz Gołębiowski
BACKGROUND: High volume of intravenous contrast in CT-angiography may result in contrast-induced nephropathy. Intraarterial ultra-low volume of contrast medium results in its satisfactory blood concentration with potentially good image quality. The first main purpose was to assess the influence of the method on function of transplanted kidney in patients with impaired graft function. The second main purpose of the study was to evaluate the usefulness of this method for detection of gastrointestinal and head-and-neck haemorrhages...
2015: Polish Journal of Radiology
Sen Wang, Jiedan Chen, Wenpan Zhang, Yan Hu, Lijing Chang, Lei Fang, Qiong Wang, Fenni Lv, Huaitong Wu, Zhanfeng Si, Shuqi Chen, Caiping Cai, Xiefei Zhu, Baoliang Zhou, Wangzhen Guo, Tianzhen Zhang
BACKGROUND: SNPs are the most abundant polymorphism type, and have been explored in many crop genomic studies, including rice and maize. SNP discovery in allotetraploid cotton genomes has lagged behind that of other crops due to their complexity and polyploidy. In this study, genome-wide SNPs are detected systematically using next-generation sequencing and efficient SNP genotyping methods, and used to construct a linkage map and characterize the structural variations in polyploid cotton genomes...
2015: Genome Biology
A Capa-Grasa, J M Rojo-Manaute, F C Rodríguez, J V Martín
BACKGROUND: Authors have reported better outcomes, by reducing surgical dissection for carpal tunnel syndromes requiring surgery. Recently, a new sonographically guided technique for ultra minimally invasive (Ultra-MIS) carpal tunnel release (CTR) through 1mm incision has been described. HYPOTHESIS: We hypothesized that a clinical trial for comparing Ultra-MIS versus Mini-open Carpal Tunnel Release (Mini-OCTR) was feasible. MATERIALS AND METHODS: To test our hypothesis, we conducted a pilot study for studying Ultra-MIS versus Mini-OCTR respectively performed through a 1mm or a 2 cm incision...
May 2014: Orthopaedics & Traumatology, Surgery & Research: OTSR
Franco Canestri
OBJECTIVE: This article describes a mathematical approach to identifying the absolute shortest laser optical focusing head f(min) associated with the smallest not-to-exceed ablative crater radius and depth to be used during ultra-conservative minimally invasive surgery (UCMIS) procedures with pulsed CO(2) lasers. This value is important because it allows forecasting the micro-boundary ablative conditions of a laser device implemented in the operating room (OR) in conjunction with minimally invasive tools...
November 2011: Photomedicine and Laser Surgery
Tobias Frauenrath, Fabian Hezel, Wolfgang Renz, Thibaut de Geyer d'Orth, Matthias Dieringer, Florian von Knobelsdorff-Brenkenhoff, Marcel Prothmann, Jeanette Schulz Menger, Thoralf Niendorf
BACKGROUND: To demonstrate the applicability of acoustic cardiac triggering (ACT) for imaging of the heart at ultrahigh magnetic fields (7.0 T) by comparing phonocardiogram, conventional vector electrocardiogram (ECG) and traditional pulse oximetry (POX) triggered 2D CINE acquisitions together with (i) a qualitative image quality analysis, (ii) an assessment of the left ventricular function parameter and (iii) an examination of trigger reliability and trigger detection variance derived from the signal waveforms...
2010: Journal of Cardiovascular Magnetic Resonance
Marzio Angelo Zullo, Alfonso Ruggiero, Roberto Montera, Francesco Plotti, Ludovico Muzii, Roberto Angioli, Pierluigi Benedetti Panici
OBJECTIVE: To evaluate use of bulking agents (polydimethylsiloxane) as an alternative treatment of female stress urinary incontinence (SUI) in older patients. STUDY DESIGN: Prospective cohort study. MAIN OUTCOME MEASURES: Women with age >75 years old, affected by stress urinary incontinence, presenting to our institution between January 2005 and January 2008 were considered for eligibility in this prospective study. All patients were submitted to transurethral implantation using Macroplastique Implantation System (MIS) (Uroplasty, Minneapolis, Minnesota)...
March 2010: Maturitas
Zhenbin Chen, Jinong Feng, Carolyn H Buzin, Qiang Liu, Lawrence Weiss, Kemp Kernstine, George Somlo, Steve S Sommer
BACKGROUND: Tumor DNA has been shown to be present both in circulating tumor cells in blood and as fragments in the plasma of metastatic cancer patients. The identification of ultra-rare tumor-specific mutations in blood would be the ultimate marker to measure efficacy of cancer therapy and/or early recurrence. Herein we present a method for detecting microinsertions/deletions/indels (MIDIs) at ultra-high analytical selectivity. MIDIs comprise about 15% of mutations. METHODS AND FINDINGS: We describe MIDI-Activated Pyrophosphorolysis (MAP), a method of ultra-high analytical selectivity for detecting MIDIs...
2009: PloS One
Tobias Frauenrath, Fabian Hezel, Uwe Heinrichs, Sebastian Kozerke, Jane F Utting, Malte Kob, Christoph Butenweg, Peter Boesiger, Thoralf Niendorf
OBJECTIVES: To circumvent the challenges of conventional electrocardiographic (ECG)-gating by examining the efficacy of an MR stethoscope, which offers (i) no risk of high voltage induction or patient burns, (ii) immunity to electromagnetic interference, (iii) suitability for all magnetic field strengths, and (iv) patient comfort together with ease of use for the pursuit of reliable and safe (ultra)high field cardiac gated magnetic resonance imaging (MRI). MATERIALS AND METHODS: The acoustic gating device consists of 3 main components: an acoustic sensor, a signal processing unit, and a coupler unit to the MRI system...
September 2009: Investigative Radiology
Kerry Ann Miller, Scott Davidson, Angela Liaros, John Barrow, Marissa Lear, Danielle Heine, Stefan Hoppler, Alasdair MacKenzie
Double knockouts of the Msx1 and Msx2 genes in the mouse result in severe cardiac outflow tract malformations similar to those frequently found in newborn infants. Despite the known role of the Msx genes in cardiac formation little is known of the regulatory systems (ligand receptor, signal transduction and protein-DNA interactions) that regulate the tissue-specific expression of the Msx genes in mammals during the formation of the outflow tract. In the present study we have used a combination of multi-species comparative genomics, mouse transgenic analysis and in-situ hybridisation to predict and validate the existence of a remote ultra-conserved enhancer that supports the expression of the Msx1 gene in migrating mouse cardiac neural crest and the outflow tract primordia...
May 15, 2008: Developmental Biology
Fred S Apple, Stephen W Smith, Lesly A Pearce, Ranka Ler, Maryann M Murakami
BACKGROUND: We determined the diagnostic accuracy of the Advia Centaur TnI-Ultra assay for detecting myocardial infarction (MI) and assessing risk of adverse events in patients presenting with ischemic symptoms suggestive of acute coronary syndrome. METHODS: We measured cardiac troponin I (cTnI) on admission and 6-24 h after admission (follow-up) in plasma specimens from 371 consecutive patients. The end point was the first of cardiac event or death within 60 days...
April 2008: Clinical Chemistry
Chee Khiang Pang, Eng Hong Ong, Guoxiao Guo, Hua Qian
Currently, the rotational speed of spindle motors in HDDs (Hard-Disk Drives) are increasing to improve high data throughput and decrease rotational latency for ultra-high data transfer rates. However, the disk platters are excited to vibrate at their natural frequencies due to higher air-flow excitation as well as eccentricities and imbalances in the disk-spindle assembly. These factors contribute directly to TMR (Track Mis-Registration) which limits achievable high recording density essential for future mobile HDDs...
January 2008: ISA Transactions
Yifei Liu, Markolf Niemz
Although lasers are nowadays widely accepted as a popular scalpel of minimally invasive surgery (MIS), one of the most common orthopedic surgeries-the replacement of the knee joint-is still performed using an ordinary oscillating saw. Since ultra-short laser pulses are usually considered to be inefficient regardless of their high precision, the newest development of femtosecond laser systems has not yet been clinically applied to any mass ablation situation. However, thin disk Yb:KYW lasers meanwhile provide sufficient output power to ablate bone tissue within a reasonable time frame...
September 2007: Lasers in Medical Science
Gautam Dantas, Alexander L Watters, Bradley M Lunde, Ziad M Eletr, Nancy G Isern, Toby Roseman, Jan Lipfert, Sebastian Doniach, Martin Tompa, Brian Kuhlman, Barry L Stoddard, Gabriele Varani, David Baker
We recently used computational protein design to create an extremely stable, globular protein, Top7, with a sequence and fold not observed previously in nature. Since Top7 was created in the absence of genetic selection, it provides a rare opportunity to investigate aspects of the cellular protein production and surveillance machinery that are subject to natural selection. Here we show that a portion of the Top7 protein corresponding to the final 49 C-terminal residues is efficiently mis-translated and accumulates at high levels in Escherichia coli...
October 6, 2006: Journal of Molecular Biology
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"