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"small animal imaging"

Andrew Brannen, Matthew Eggert, Matthias Nahrendorf, Robert Arnold, Peter Panizzi
Pre-clinical monitoring of tumor growth and identification of distal metastasis requires a balance between accuracy and expediency. Bioluminescence imaging (BLI) is often used to track tumor growth but is primarily limited to planar 2-dimensional (2D) imaging. Consistent subject placement within a standard top-mounted, single-detector small animal imager is vital to reducing variability in repeated same-animal measures over time. Here, we describe a method for tracking tumor development using a multi-angle BLI and photo-acoustic workflow...
February 20, 2018: Scientific Reports
Christin Neuber, Birgit Belter, Sebastian Meister, Frank Hofheinz, Ralf Bergmann, Hans-Jürgen Pietzsch, And Jens Pietzsch
Experimental evidence has associated receptor tyrosine kinase EphB4 with tumor angiogenesis also in malignant melanoma. Considering the limited in vivo data available, we have conducted a systematic multitracer and multimodal imaging investigation in EphB4-overexpressing and mock-transfected A375 melanoma xenografts. Tumor growth, perfusion, and hypoxia were investigated by positron emission tomography. Vascularization was investigated by fluorescence imaging in vivo and ex vivo. The approach was completed by magnetic resonance imaging, radioluminography ex vivo, and immunohistochemical staining for blood and lymph vessel markers...
February 17, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Stéphanie Bricq, Hiliwi Leake Kidane, Jorge Zavala-Bojorquez, Alexandra Oudot, Jean-Marc Vrigneaud, François Brunotte, Paul Michael Walker, Alexandre Cochet, Alain Lalande
PET images deliver functional data, whereas MRI images provide anatomical information. Merging the complementary information from these two modalities is helpful in oncology. Alignment of PET/MRI images requires the use of multi-modal registration methods. Most of existing PET/MRI registration methods have been developed for humans and few works have been performed for small animal images. We proposed an automatic tool allowing PET/MRI registration for pre-clinical study based on a two-level hierarchical approach...
February 7, 2018: Medical & Biological Engineering & Computing
Robert H Wilson, Christian Crouzet, Mohammad Torabzadeh, Afsheen Bazrafkan, Maryam H Farahabadi, Babak Jamasian, Dishant Donga, Juan Alcocer, Shuhab M Zaher, Bernard Choi, Yama Akbari, Bruce J Tromberg
Quantifying rapidly varying perturbations in cerebral tissue absorption and scattering can potentially help to characterize changes in brain function caused by ischemic trauma. We have developed a platform for rapid intrinsic signal brain optical imaging using macroscopically structured light. The device performs fast, multispectral, spatial frequency domain imaging (SFDI), detecting backscattered light from three-phase binary square-wave projected patterns, which have a much higher refresh rate than sinusoidal patterns used in conventional SFDI...
October 2017: Neurophotonics
Gemma M Dias, Caterina F Ramogida, Julie Rousseau, Nicholas A Zacchia, Cornelia Hoehr, Paul Schaffer, Kuo-Shyan Lin, François Bénard
INTRODUCTION: Zirconium-89 (89Zr, t1/2=78.4h) liquid target (LT) production offers an approach to introduce this positron-emitting isotope to cyclotron centres without the need for a separate solid target (ST) production set up. We compared the production, purification, and antibody radiolabeling yields of 89Zr-(LT) and 89Zr-(ST), and assessed the feasibility of 89Zr-(LT) for preclinical PET/CT. METHODS: 89Zr-(ST) production was performed with an 89Y foil on a TR 19 cyclotron at 13...
November 16, 2017: Nuclear Medicine and Biology
Mihaela Ghita, Stephen J McMahon, Hannah F Thompson, Conor K McGarry, Raymond King, Sarah O S Osman, Jonathan L Kane, Amanda Tulk, Giuseppe Schettino, Karl T Butterworth, Alan R Hounsell, Kevin M Prise
BACKGROUND: Preclinical radiation biology has become increasingly sophisticated due to the implementation of advanced small animal image guided radiation platforms into laboratory investigation. These small animal radiotherapy devices enable state-of-the-art image guided therapy (IGRT) research to be performed by combining high-resolution cone beam computed tomography (CBCT) imaging with an isocentric irradiation system. Such platforms are capable of replicating modern clinical systems similar to those that integrate a linear accelerator with on-board CBCT image guidance...
December 28, 2017: Radiation Oncology
Tasuku Kiyuna, Yasunori Tome, Fuminari Uehara, Takashi Murakami, Yong Zhang, Ming Zhao, Fuminori Kanaya, Robert M Hoffman
BACKGROUND: We previously developed a color-coded imaging model that can quantify the length of nascent blood vessels using Gelfoam® implanted in nestin-driven green fluorescent protein (ND-GFP) nude mice. In this model, nascent blood vessels selectively express GFP. We also previously showed that osteosarcoma cells promote angiogenesis in this assay. We have also previously demonstrated the tumor-targeting bacteria Salmonella typhimurium A1-R (S. typhimurium A1-R) can inhibit or regress all tested tumor types in mouse models...
January 2018: Anticancer Research
Ali Hariri, Jeanne Lemaster, Junxin Wang, AnanthaKrishnan S Jeevarathinam, Daniel L Chao, Jesse V Jokerst
Photoacoustic imaging (PAI) is a non-invasive, high-resolution hybrid imaging modality that combines optical excitation and ultrasound detection. PAI can image endogenous chromophores (melanin, hemoglobin, etc.) and exogenous contrast agents in different medical applications. However, most current equipment uses sophisticated and complicated OPO lasers with tuning and stability features inconsistent with broad clinical deployment. As the number of applications of PAI in medicine increases, there is an urgent need to make the imaging equipment more compact, portable, and affordable...
March 2018: Photoacoustics
Payvand Taherparvar, Alireza Sadremomtaz
GATE is currently considered in scintigraphic imaging as a powerful tool to develop, design and optimize nuclear medicine modalities. This paper describes the GATE simulation of a pixelated gamma camera which is dedicated to high resolution of small animals imaging. It consists of a CsI(Na) crystal array coupled to position sensitive photomultiplier tube. The simulation model includes photon tracking through low energy high resolution hexagonal parallel holes collimator, CsI(Na) pixelated crystal, back-compartment, and camera shielding...
March 2018: Australasian Physical & Engineering Sciences in Medicine
Jeffrey A Ketterling, Orlando Aristizábal, Billy Y S Yiu, Daniel H Turnbull, Colin K L Phoon, Alfred C H Yu, Ronald H Silverman
Real-time imaging of the embryonic murine cardiovascular system is challenging due to the small size of the mouse embryo and rapid heart rate. High-frequency, linear-array ultrasound systems designed for small-animal imaging provide high-frame-rate and Doppler modes but are limited in regards to the field of view that can be imaged at fine-temporal and -spatial resolution. Here, a plane-wave imaging method was used to obtain high-speed image data from in utero mouse embryos and multi-angle, vector-flow algorithms were applied to the data to provide information on blood flow patterns in major organs...
November 30, 2017: Scientific Reports
Joshua J Yim, Martina Tholen, Alwin Klaassen, Jonathan Sorger, Matthew Bogyo
Molecularly targeted optical contrast agents have the potential to enable surgeons to visualize specific molecular markers that can help improve surgical precision and thus outcomes. Fluorescently quenched substrates can be used to highlight tumor lesions by targeting proteases that are highly abundant in the tumor microenvironment. However, the majority of these and other molecularly targeted optical contrast agents are labeled with reporter dyes that are not ideally matched to the properties of clinical camera systems, which are typically optimized for detection of indocyanine-green (ICG)...
December 14, 2017: Molecular Pharmaceutics
Song Yeul Lee, Min Lin, Aeju Lee, Yong Il Park
Lanthanide-doped nanoparticles exhibit unique optical properties, such as a long luminescence lifetime (up to several milliseconds), sharp emission peaks, and upconversion luminescence over the range of wavelengths from near-infrared to visible. Exploiting these optical properties, lanthanide-doped nanoparticles have been widely utilized for cellular and small animal imaging with the absence of background autofluorescence. In addition, these nanoparticles have advantages of high signal-to-noise ratio for highly sensitive and selective diagnostic detection...
November 23, 2017: Nanomaterials
Y Y Liu, C Wang, P F Luo, Z F Xia
Objective: To establish 3-{4-[2-hydroxyl-(1-methylethylamino) propoxy] phenyl} propionic acid cetylesters (PAC) modified nanoparticles, and preliminarily explore its cardiomyocyte-targeting function and protection effects on myocardium. Methods: (1) HL-1 myocardial cells were divided into cyanidin-3 (Cy3) marked non-targeted small interference RNA (Cy3-siNC) group and Cy3 marked small interference RNA designed for the nuclear factor kappa B (NF-κB)-p65 gene (Cy3-si435) group according to the random number table, with 3 wells in each group...
November 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
Kathyayini Sivasubramanian, Vijitha Periyasamy, Manojit Pramanik
Translation of photoacoustic imaging into the clinic is a major challenge. Handheld real-time clinical photoacoustic imaging systems are very rare. Here, we report a combined photoacoustic and clinical ultrasound imaging system by integrating an ultrasound probe with light delivery for small animal imaging. We demonstrate this by showing sentinel lymph node imaging in small animals along with minimally invasive real-time needle guidance. A clinical ultrasound platform with access to raw channel data allows the integration of photoacoustic imaging leading to a handheld real-time clinical photoacoustic imaging system...
October 16, 2017: Journal of Visualized Experiments: JoVE
Lili Zhao, Jiehui Jiang, Yuexia Shu, Zhuangzhi Yan, Xin Liu
Cone-beam X-ray luminescence optical tomography (CB-XLOT) plays an important role in in vivo small animal imaging study, which can non-invasively image the three-dimensional (3-D) distribution of x-ray-excitable nanophosphors deeply embedded in imaged object. However, CB-XLOT suffers from a low spatial resolution due to the ill-posed nature of optical reconstruction. To alleviate the ill-posedness of reconstruction and improve the imaging performance of XLOT, in this paper, we propose an iterative weighted L1 minimization method which is achieved by incorporating YALL1 (Your algorithm for L1 norm problems)...
July 2017: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
Tiffany C Kwong, Farouk Nouizi, Jaedu Cho, Yuting Lin, Uma Sampathkumaran, Gultekin Gulsen
Previously, we demonstrated that temperature-modulated fluorescence tomography (TM-FT) could provide fluorescence images with high quantitative accuracy and the spatial resolution of focused ultrasound. TM-FT is based on scanning the focused ultrasound across the medium to activate temperature-reversible fluorescent nanoprobes (ThermoDots). This technique can resolve small fluorescent targets located several centimeters deep in turbid media with millimeter resolution. Our past studies with this multimodality technique used agar phantoms, which could not represent the true heterogeneous nature of the acoustic and optical properties of biological tissue...
October 1, 2017: Applied Optics
Anchal Ghai, Dolonchampa Maji, Nicholas Cho, Chantiya Chanswangphuwana, Michael Rettig, John DiPersio, Walter Akers, Farrokh Dehdashti, Samuel Achilefu, Ravi Vij, Monica Shokeen
Multiple myeloma (MM) is a plasma B-cell hematologic cancer that causes significant skeletal morbidity. Despite improvements in survival, heterogeneity in response remains a major challenge in MM. Cluster of differentiation 38 (CD38) is a type II transmembrane glycoprotein over-expressed in myeloma cells and is implicated in MM cell signaling. Daratumumab is US Food and Drug Administration approved high-affinity monoclonal antibody targeting CD38 that is clinically benefiting refractory MM patients. Here, we evaluated [89Zr]Zr-DFO-daratumumab positron emission tomography/computed tomography (PET/CT) imaging in MM tumor models...
October 12, 2017: Journal of Nuclear Medicine: Official Publication, Society of Nuclear Medicine
Christine R Lattin, Maxwell A Emerson, Jean-Dominique Gallezot, Tim Mulnix, J Elliott Brown, Richard E Carson
BACKGROUND: One potential barrier to using in vivo imaging in any new animal species is solving the basic problem of how to hold animals safely and securely during scans. NEW METHOD: In this paper, we describe the design, fabrication, use, and positional reproducibility of a 3D-printed plastic device (the Avian Imaging Device, or AID) for imaging the brain of 1 or 2 small songbirds. We designed two different types of head cones to use with this device: one that was not contoured and designed for anesthesia induction, and one contoured to the shape of a house sparrow head, designed to be used with a pre-anesthetized animal...
January 1, 2018: Journal of Neuroscience Methods
Jonathan C DeLong, Takashi Murakami, Paul J Yazaki, Robert M Hoffman, Michael Bouvet
BACKGROUND: The success of a curative surgery for cancer is dependent on the complete removal of all cancer cells. Tumor visualization by the surgeon can be enhanced through fluorescent-antibody targeting. To further develop such technology, we selected humanized anti-carcinoembryonic antigen (CEA) conjugated to a near-infrared dye to target orthotopically-implanted human colon cancer in nude mice. MATERIALS AND METHODS: The HT-29 human colon cancer cell line was grown in culture and subcutaneously injected in mice...
October 2017: Journal of Surgical Research
Zihui Li, Ralph Müller, Davide Ruffoni
Anchorage of orthopedic implants depends on the interfacial bonding between the implant and the host bone as well as on the mass and microstructure of peri-implant bone, with all these factors being continuously regulated by the biological process of bone (re)modeling. In osteoporotic bone, implant integration may be jeopardized not only by lower peri-implant bone quality but also by reduced intrinsic regeneration ability. The first aim of this review is to provide a critical overview of the influence of osteoporosis on bone regeneration post-implantation...
October 3, 2017: Journal of Orthopaedic Research: Official Publication of the Orthopaedic Research Society
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