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https://www.readbyqxmd.com/read/28342671/apoptosis-of-peritoneal-leucocytes-during-early-stages-of-fasciola-hepatica-infections-in-sheep
#1
A Escamilla, R Pérez-Caballero, R Zafra, M J Bautista, I L Pacheco, M T Ruiz, M S Martínez-Cruz, A Martínez-Moreno, V Molina-Hernández, J Pérez
Several immunomodulatory properties have been described in Fasciola hepatica infections. Apoptosis has been shown to be an effective mechanism to avoid the immune response in helminth infections. The aim of the present work was to study apoptosis in peritoneal leucocytes of sheep experimentally infected with F. hepatica during the early stages of infection. Five groups (n=5) of sheep were used. Groups 2-5 were orally infected with 200 metacercariae (mc) and sacrificed at 1, 3, 9 and 18days post-infection (dpi), respectively...
March 18, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28333957/immune-response-of-rats-vaccinated-orally-with-various-plant-expressed-recombinant-cysteine-proteinase-constructs-when-challenged-with-fasciola-hepatica-metacercariae
#2
Malgorzata Kesik-Brodacka, Agnieszka Lipiec, Monika Kozak Ljunggren, Luiza Jedlina, Katarzyna Miedzinska, Magdalena Mikolajczak, Andrzej Plucienniczak, Andrzej B Legocki, Halina Wedrychowicz
BACKGROUND: Cysteine proteinases of Fasciola hepatica are important candidates for vaccine antigens because of their role in fluke biology and host-parasite relationships. In our previous experiments, we found that a recombinant cysteine proteinase cloned from adult F. hepatica (CPFhW) can protect rats against liver fluke infections when it is administered intramuscularly or intranasally in the form of cDNA. We also observed considerable protection upon challenge following mucosal vaccination with inclusion bodies containing recombinant CPFhW produced in Escherichia coli...
March 23, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28327829/-western-blot-diagnostic-yield-for-simultaneous-antibody-detection-in-patients-with-human-cysticercosis-hydatidosis-and-human-fascioliasis
#3
Kelly Davelois, Hermes Escalante, César Jara
. To determine the diagnostic yield using western blotting to simultaneously detect antibodies in patients with human cysticercosis, hydatidosis, and human fascioliasis. Materials and methods . Cross-sectional study of diagnostic yield assessment. Excretory/secretory antigens were obtained from Taenia solium larvae, Echinococcus granulosus cysts, and the adult flukes of Fasciola hepática, which were then separated using the polyacrylamide gel electrophoresis technique, transferred, and attached to a nitrocellulose membrane to be probed with sera from the patient infected with the three parasites...
October 2016: Revista Peruana de Medicina Experimental y Salud Pública
https://www.readbyqxmd.com/read/28300559/expression-purification-and-in-vitro-refolding-of-the-recombinant-truncated-saposin-like-protein-2-antigen-for-development-of-diagnosis-of-human-fascioliasis
#4
Abolfazl Mirzadeh, Zarrintaj Valadkhani, Asiyeh Yoosefy, Jalal Babaie, Majid Golkar, Ahmad Reza Esmaeili Rastaghi, Elham Kazemi-Rad, Keyhan Ashrafi
Early diagnosis of fascioliasis is critical in prevention of injury to the liver and bile ducts. Saposin-like protein (FhSAP-2) is probably the most ideal antigen of Fasciola hepatica for development of ELISA kits. SAP-2 has a conserved tertiary structure containing three disulfide bonds and conformational epitopes. Therefore, antigenicity of SAP-2 is greatly depends on disulfide bond formation and proper folding. We produced the recombinant truncated SAP-2 (rtSAP-2) in the SHuffle® T7 and Rosetta strain of E...
March 11, 2017: Acta Tropica
https://www.readbyqxmd.com/read/28285514/fasciola-hepatica-infection-status-of-freshwater-snails-collected-from-gangwon-do-province-korea
#5
Jae-Hyung Lee, Juan-Hua Quan, In-Wook Choi, Gab-Man Park, Guang-Ho Cha, Hyun-Ju Kim, Jae-Min Yuk, Young-Ha Lee
Fasciola hepatica is a trematode that causes zoonosis, mainly in cattle and sheep, and occasionally in humans. Few recent studies have determined the infection status of this fluke in Korea. In August 2015, we collected 402 samples of freshwater snails at Hoenggye-ri (upper stream) and Suha-ri (lower stream) of Song-cheon (stream) in Daegwalnyeong-myeon, Pyeongchang-gun in Gangwon-do (Province) near many large cattle or sheep farms. F. hepatica infection was determined using PCR on the nuclear ribosomal internal transcribed spacer 2 (ITS-2)...
February 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28285513/seroprevalence-and-risk-factors-of-fascioliasis-in-yaks-bos-grunniens-from-three-counties-of-gansu-province-china
#6
Xiao-Xuan Zhang, Sheng-Yong Feng, Jian-Gang Ma, Wen-Bin Zheng, Ming-Yang Yin, Si-Yuan Qin, Dong-Hui Zhou, Quan Zhao, Xing-Quan Zhu
The aim of this study was to determine the seroprevalence and risk factors of fascioliasis in yaks, Bos grunniens, from 3 counties of Gansu Province in China. A total of 1,584 serum samples, including 974 samples from white yaks from Tianzhu, 464 from black yaks from Maqu, and 146 from black yaks from Luqu County, were collected and analyzed using ELISA to detect IgG antibodies against Fasciola hepatica. The overall F. hepatica seroprevalence was 28.7% (454/1,584), with 29.2% in white yaks (284/974) and 27...
February 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28285507/human-cases-of-fascioliasis-in-fujian-province-china
#7
Lin Ai, Yu-Chun Cai, Yan Lu, Jia-Xu Chen, Shao-Hong Chen
Fascioliasis is a foodborne zoonotic parasitic disease. We report 4 cases occurring in the same family, in whom diagnosis of acute fascioliasis was established after series of tests. One case was hospitalized with fever, eosinophilia, and hepatic lesions. MRI showed hypodense changes in both liver lobes. The remaining 3 cases presented with the symptom of stomachache only. Stool analysis was positive for Fasciola eggs in 2 adult patients. The immunological test and molecular identification of eggs were confirmed at the National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, China...
February 2017: Korean Journal of Parasitology
https://www.readbyqxmd.com/read/28272650/fascioliasis-a-rare-parasitic-infection-mimicking-tumor-in-the-liver-report-of-two-cases
#8
Emine Şamdanci, Nurhan Şahin, Adile Ferda Dağli, Ayşe Nur Akatli, Nasuhi Engin Aydin
Fascioliasis is a rarely encountered parasitic infection in Turkey that mainly affects the liver and bile ducts. Other defined localizations of the parasite are the lungs, gastrointestinal system, and subcutaneous fatty tissue. Two cases of female patients who presented to the hospital with abdominal pain and whose physical examination and laboratory findings were normal except peripheral eosinophilia, were detected to have liver masses with necrotic areas. Segmental hepatectomies were performed in both cases with the preliminary diagnosis of liver tumors...
February 4, 2017: Türk Patoloji Dergisi
https://www.readbyqxmd.com/read/28242355/activities-of-ectonucleotidases-and-adenosine-deaminase-in-platelets-of-cattle-experimentally-infected-by-fasciola-hepatica
#9
Mateus Fracasso, Aleksandro S Da Silva, Matheus D Baldissera, Nathieli B Bottari, Mateus E Gabriel, Manoela M Piva, Fernanda A Stedille, Ricardo Christ, Leandro A Rhoden, Luan C Henker, Vera M Moresch, Maria Rosa C Schetinger, Ricardo E Mendes
The enzymatic activities of NTPDase, 5'-nucleotidase and adenosine deaminase (ADA) are important in regulating the concentration of adenine nucleotides, molecules known to be involved on platelet aggregation. Fasciolosis causes coagulation disorders that have not been completely elucidated. Taking into consideration the association between the purinergic system and hemostasis, this study aimed to evaluate the enzymatic activities of NTPDase (hydrolyze ATP and ADP), 5'-nucleotidase (hydrolyze AMP) and ADA (deamination of adenosine) in platelets from cattle experimentally infected by Fasciola hepatica on days 20, 40, 60 and 80 post-infection (PI)...
February 24, 2017: Experimental Parasitology
https://www.readbyqxmd.com/read/28228149/fasciolopsis-buski-digenea-fasciolidae-from-china-and-india-may-represent-distinct-taxa-based-on-mitochondrial-and-nuclear-ribosomal-dna-sequences
#10
Jun Ma, Miao-Miao Sun, Jun-Jun He, Guo-Hua Liu, Lin Ai, Mu-Xin Chen, Xing-Quan Zhu
BACKGROUND: Fasciolopsis buski is a zoonotic intestinal fluke infecting humans and pigs, but it has been seriously neglected. It is yet to know whether there is any genetic diversity among F. buski from different geographical locations, particularly in sequences of nuclear ribosomal DNA (rDNA) and mitochondrial (mt) DNA. Therefore, we determined the sequences of partial 18S, the complete internal transcribed spacer (ITS) rDNA and the complete mt genome of F. buski from China, compared the rDNA and mtDNA sequences with those of isolates from India and Vietnam, and assessed the phylogenetic relationships of this fluke and related fasciolid trematodes based on the mtDNA dataset...
February 22, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28215874/first-paleoparasitological-record-of-digenean-eggs-from-a-native-deer-from-patagonia-argentina-cueva-parque-diana-archaeological-site
#11
Beltrame María Ornela, Tietze Eleonor, Pérez Alberto Enrique, Sardella Norma Haydeé
Eggs representative of a digenean species were found in coprolites belonged to an endemic deer from Patagonia. Samples were collected from the archaeological site named "Cueva Parque Diana". This site is a cave located at the Lanín National Park, Neuquén Province, Argentina. The coprolites were dated from 2370±70 to 580±60 years B.P. The eggs were ellipsoidal, operculated, yellowish and thin-shelled. Measurements (n=65) ranged from 120.0 to 142.5 (133.2±6.53) μm long and 62.5 to 87.5 (72.6±6.15) μm wide...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28186433/production-of-monoclonal-antibody-against-excretory-secretory-antigen-of-fasciola-hepatica-and-evaluation-of-its-efficacy-in-the-diagnosis-of-fascioliasis
#12
Samaneh Abdolahi Khabisi, Bahador Sarkari, Abdolali Moshfe, Sedigheh Jalali
Parasitological methods are not helpful for the diagnosis of fascioliasis in acute and invasive periods of the disease. Detection of coproantigens seems to be a suitable alternative approach in the diagnosis of fascioliasis. The present study aimed to develop a reliable antigen detection system, using monoclonal antibodies raised against excretory-secretory (ES) antigen of Fasciola hepatica, for the diagnosis of fascioliasis. Fasciola adult worms were collected from the bile ducts of infected animals. Species of the fluke was determined by polymerase chain reaction-restriction fragment length polymorphism (RFLP-PCR)...
February 2017: Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
https://www.readbyqxmd.com/read/28162101/fasciola-hepatica-infections-in-cattle-and-the-freshwater-snail-galba-truncatula-from-dakhla-oasis-egypt
#13
W M Arafa, A I Hassan, S A M Snousi, Kh M El-Dakhly, P J Holman, T M Craig, S M Aboelhadid
Infection by Fasciola species was investigated in seven districts of Dakhla Oasis, Egypt, through abattoir inspection of cattle livers for adult worms and sedimentation of faecal samples from local cattle to detect Fasciola eggs. In addition, lymnaeid snails collected from the study area were examined microscopically for developmental stages of Fasciola spp. Abattoir inspection revealed that 51 out of 458 cattle livers (11.1%) contained adult flukes, which were identified morphologically as Fasciola hepatica...
February 6, 2017: Journal of Helminthology
https://www.readbyqxmd.com/read/28158188/dna-multigene-characterization-of-fasciola-hepatica-and-lymnaea-neotropica-and-its-fascioliasis-transmission-capacity-in-uruguay-with-historical-correlation-human-report-review-and-infection-risk-analysis
#14
María Dolores Bargues, Valeria Gayo, Jaime Sanchis, Patricio Artigas, Messaoud Khoubbane, Soledad Birriel, Santiago Mas-Coma
BACKGROUND: Fascioliasis is a pathogenic disease transmitted by lymnaeid snails and recently emerging in humans, in part due to effects of climate changes, anthropogenic environment modifications, import/export and movements of livestock. South America is the continent presenting more human fascioliasis hyperendemic areas and the highest prevalences and intensities known. These scenarios appear mainly linked to altitude areas in Andean countries, whereas lowland areas of non-Andean countries, such as Uruguay, only show sporadic human cases or outbreaks...
February 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28152102/anthelmintic-potential-of-thymoquinone-and-curcumin-on-fasciola-gigantica
#15
Rizwan Ullah, Abdur Rehman, Mohd Faraz Zafeer, Lubna Rehman, Yasir A Khan, M A Hannan Khan, Shahper N Khan, Asad U Khan, S M A Abidi
Fasciolosis an economically important global disease of ruminants in the temperate and tropical regions, caused by Fasciola hepatica and F. gigantica, respectively, also poses a potential zoonotic threat. In India alone it causes huge losses to stakeholders. Anthelmintics including triclabendazole have been used to control this menace but the emerging resistance against the available compounds necessitates identification of novel and alternative therapeutic measures involving plant derived natural compounds for their anthelmintic potential...
2017: PloS One
https://www.readbyqxmd.com/read/28145217/rumen-fluke-calicophoron-daubneyi-on-welsh-farms-prevalence-risk-factors-and-observations-on-co-infection-with-fasciola-hepatica
#16
Rhys Aled Jones, Peter M Brophy, E Sian Mitchell, Hefin Wyn Williams
Reports of Calicophoron daubneyi infecting livestock in Europe have increased substantially over the past decade; however, there has not been an estimate of its farm level prevalence and associated risk factors in the UK. Here, the prevalence of C. daubneyi across 100 participating Welsh farms was recorded, with climate, environmental and management factors attained for each farm and used to create logistic regression models explaining its prevalence. Sixty-one per cent of farms studied were positive for C...
February 2017: Parasitology
https://www.readbyqxmd.com/read/28114888/transcriptome-profiling-of-gene-expression-during-immunisation-trial-against-fasciola-hepatica-identification-of-genes-and-pathways-involved-in-conferring-immunoprotection-in-a-murine-model
#17
Jose Rojas-Caraballo, Julio López-Abán, Darwin Andrés Moreno-Pérez, Belén Vicente, Pedro Fernández-Soto, Esther Del Olmo, Manuel Alfonso Patarroyo, Antonio Muro
BACKGROUND: Fasciolosis remains a significant food-borne trematode disease causing high morbidity around the world and affecting grazing animals and humans. A deeper understanding concerning the molecular mechanisms by which Fasciola hepatica infection occurs, as well as the molecular basis involved in acquiring protection is extremely important when designing and selecting new vaccine candidates. The present study provides a first report of microarray-based technology for describing changes in the splenic gene expression profile for mice immunised with a highly effective, protection-inducing, multi-epitope, subunit-based, chemically-synthesised vaccine candidate against F...
January 23, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28103965/oxidative-stress-and-changes-in-adenosine-deaminase-activity-of-cattle-experimentally-infected-by-fasciola-hepatica
#18
Aleksandro S DA Silva, Matheus D Baldissera, Nathieli B Bottari, Matheus E Gabriel, Leandro A Rhoden, Manoela M Piva, Ricardo Christ, Fernanda A Stedille, Anderson Gris, Vera M Morsch, Maria Rosa Schetinger, Ricardo E Mendes
The aim of this study was to evaluate the oxidative stress in serum and liver and adenosine deaminase (ADA) activity of cattle experimentally infected by Fasciola hepatica. The group A consisted of five healthy animals (uninfected), and the group B was composed of five animals orally infected with 200 metacercariae of F. hepatica. On days 20, 40, 60 and 80 post-infection (PI) serum was collected to measure oxidative stress variables. On day 100 PI, animals were humanely euthanized and liver samples were collected...
January 20, 2017: Parasitology
https://www.readbyqxmd.com/read/28096855/helminth-infections-of-meriones-persicus-persian-jird-mus-musculus-house-mice-and-cricetulus-migratorius-grey-hamster-a-cross-sectional-study-in-meshkin-shahr-district-northwest-iran
#19
Zabiholah Zarei, Mehdi Mohebali, Zahra Heidari, Jaber Davoodi, Afshin Shabestari, Afsaneh Motevalli Haghi, Khadijeh Khanaliha, Eshrat Beigom Kia
BACKGROUND: Rodents have important role as reservoirs of different parasites. The aim of this study was to determine helminth parasites of abundant rodents in Meshkin-Shahr, Ardabil Province northwest Iran. METHODS: From April 2014 to March 2015; 205 rodents including 118 Meriones persicus, 63 Mus musculus and 24 Cricetulus migratorius were collected, using live traps. All rodents were dissected and their different tissues examined for infectivity with helminth parasites...
April 2016: Iranian Journal of Parasitology
https://www.readbyqxmd.com/read/28088688/randomized-phase-iii-study-to-assess-efficacy-and-safety-of-adjuvant-capox-with-or-without-bevacizumab-in-patients-after-resection-of-colorectal-liver-metastases-hepatica-study
#20
Nikol Snoeren, Richard van Hillegersberg, Sander B Schouten, Andre M Bergman, Erikv van Werkhoven, Otilia Dalesio, Rob A E M Tollenaar, Henk M Verheul, Joost van der Sijp, Inne H M Borel Rinkes, E E Voest
Bevacizumab is a humanized monoclonal antibody targeting vascular endothelial growth factor (VEGF). Recurrence after resection of colorectal liver metastases (CRLMs), presumably caused by VEGF-mediated outgrowth of micrometastases, might decrease when VEGF is inhibited. This study examines the efficacy and safety of adding bevacizumab to an adjuvant regimen of CAPOX in patients undergoing radical resection for their CRLMs. Patients with resected CRLMs were randomized after surgery to receive CAPOX and bevacizumab (arm A) or CAPOX alone (arm B) as adjuvant treatment...
February 2017: Neoplasia: An International Journal for Oncology Research
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