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https://www.readbyqxmd.com/read/29232690/rna-interference-in-fasciola-gigantica-establishing-and-optimization-of-experimental-rnai-in-the-newly-excysted-juveniles-of-the-fluke
#1
Arun Anandanarayanan, Opinder Krishen Raina, Hniang Lalrinkima, Ajayta Rialch, Muthu Sankar, Anju Varghese
Fasciolosis caused by Fasciola gigantica is a neglected tropical disease but a constraint on the growth and productivity of cattle, buffaloes and sheep in the tropical countries of Asia and Africa. Resistance to commonly used anthelmintics in Fasciola has increased the need to search for alternative therapeutic targets. RNA interference is the current tool of choice in the search for such targets in Fasciola. The susceptibility of juvenile Fasciola hepatica to double stranded (ds) RNA induced RNAi has been established but in F...
December 12, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29221457/capillaria-hepatica-calodium-hepaticum-infection-in-a-horse-a-case-report
#2
Akihiro Ochi, Tatsuro Hifumi, Takanori Ueno, Yoshinari Katayama
BACKGROUND: Capillaria hepatica is a zoonotic parasite in humans and animals and has a worldwide distribution. However, infections in mammals apart from rodents, which are natural hosts of the parasite, have rarely been reported. This report describes the first known case of C. hepatica infection in a horse in Japan. CASE PRESENTATION: A 3-year-old filly without clinical signs was presented at a slaughterhouse in Japan. Gross examination revealed white to tan nodules 0...
December 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29199372/comparative-assessment-of-recombinant-and-native-immunogenic-forms-of-fasciola-hepatica-proteins-for-serodiagnosis-of-sheep-fasciolosis
#3
Kobra Mokhtarian, Ahmad Reza Meamar, Majid Khoshmirsafa, Elham Razmjou, Leila Masoori, Majid Khanmohammadi, Lame Akhlaghi, Reza Falak
Laboratory diagnosis of sheep fasciolosis is commonly performed by coprological examinations; however, this method may lead to false negative results during the acute phase of the infection. Furthermore, the poor sensitivity of coprological methods is considered to be a paradox in the chronic phase of the infection. In this study, we compared the immunoreactivity of native and recombinant forms of Fasciola hepatica excretory/secretory antigens and determined their capabilities for the development of F. hepatica-specific immunoassays...
December 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29187710/hybrid-origin-of-asian-aspermic-fasciola-flukes-is-confirmed-by-analyzing-two-single-copy-genes-pepck-and-pold
#4
Kei Hayashi, Madoka Ichikawa-Seki, Uday Kumar Mohanta, Takuya Shoriki, Pannigan Chaichanasak, Tadashi Itagaki
Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages for obtaining a definitive evidence of the hybrid origin of aspermic Fasciola flukes. All the flukes categorized into the aspermic nad1 lineages possessed both the fragment patterns of F...
November 29, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29185852/lethal-fascioliasis-in-capybaras-hydrochoerus-hydrochaeris-in-brazil
#5
Marcelo Bahia Labruna, Francisco Costa, Marcio Port-Carvalho, Alice Oliveira, Silvio Souza, Marcio Castro
The liver fluke Fasciola hepatica (Trematoda: Fasciolidae) causes fascioliasis, which affects mostly domestic ruminants and humans worldwide. This parasite has an Old World origin and was introduced into the New World by European colonizers. Capybara (Hydrochoerus hydrochaeris) is the largest living rodent species, with adults weighing over 60 kg. We report a fascioliasis outbreak caused by F. hepatica, that reduced a capybara group from 21 to 2 animals within a 9-mo period. Animal infection and associated lesions were confirmed by post-mortem examinations, which revealed extensive liver damage associated with the presence of large number of adult and immature forms of F...
November 29, 2017: Journal of Parasitology
https://www.readbyqxmd.com/read/29173540/characterization-of-multiple-life-stages-of-two-australian-fasciola-hepatica-isolates-in-sheep
#6
S D George, K Baker, L Lake, K Vanhoff, R D'Arcy, D Emery, P F Rolfe
Information on the susceptibility status of Fasciola hepatica isolates is lacking in the literature, even for those isolates considered to be laboratory reference strains. Four controlled efficacy studies were conducted on two Fasciola hepatica isolates from Australia, viz. 'Oberon' and 'Sunny Corner' with treatment at either 2, 6 or 10 weeks post-infection (wpi) as defined in each study. Fluke burdens and examination of livers occurred at necropsy in weeks 12 (Study 1) or 13 (Studies 2, 3 and 4) post-infection...
December 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/29169803/expression-purification-and-characterization-of-two-leucine-aminopeptidases-of-the-blood-fluke-schistosoma-mansoni
#7
Gabriela Maggioli, Gabriel Rinaldi, Ines Giaudrone, Patricia Berasain, José F Tort, Paul J Brindley, Carlos Carmona
Schistosomiasis is a major neglected tropical disease (NTD) and considered the most important of the human helminthiases in terms of morbidity and mortality. Whereas treatment with praziquantel has been effective since the 1980s, the potential for the emergence of drug resistance has propelled the search for new interventions. Studies have revealed key roles of proteases in parasitic helminths during establishment of infection, tissue invasion, immune evasion, parasite feeding and development throughout the different developmental stages, pinpointing them as possible candidates...
November 20, 2017: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29161330/fasciola-spp-mapping-of-the-mf6-epitope-and-antigenic-analysis-of-the-mf6p-hdm-family-of-heme-binding-proteins
#8
Victoria Martínez-Sernández, María J Perteguer, Mercedes Mezo, Marta González-Warleta, Teresa Gárate, M Adela Valero, Florencio M Ubeira
MF6p/FhHDM-1 is a small cationic heme-binding protein which is recognized by the monoclonal antibody (mAb) MF6, and abundantly present in parenchymal cells and secreted antigens of Fasciola hepatica. Orthologs of this protein (MF6p/HDMs) also exist in other causal agents of important foodborne trematodiasis, such as Clonorchis sinensis, Opisthorchis viverrini and Paragonimus westermani. Considering that MF6p/FhHDM-1 is relevant for heme homeostasis in Fasciola and was reported to have immunomodulatory properties, this protein is expected to be a useful target for vaccination...
2017: PloS One
https://www.readbyqxmd.com/read/29160354/dynamics-of-haemocytes-from-pseudosuccinea-columella-circulating-infected-by-fasciola-hepatica
#9
Vinicius Marques Antunes Ribeiro, Aytube Lucas Coaglio, Fernando Luiz Pereira Oliveira, Cíntia Aparecida de Jesus Pereira, Walter Dos Santos Lima
The lymnaeids are important in the epidemiology of Fasciola hepatica, a neglected and endemic zoonosis. The interaction between the internal defense system of Pseudosuccinea columella and F. hepatica has been little studied. In the present study the effect of infection by F. hepatica on P. columella circulating haemocytes was investigated. Changes in the average number of total circulating haemocytes have been observed at 30 minutes post-infection and 1, 7, 10, 14, 21, 28 and 50 days post-infection (dpi). Miracidia were observed head-foot and mantle at 30 minutes post-infection...
November 9, 2017: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/29158867/hepatic-fascioliasis-presenting-with-bile-duct-obstruction-a-case-report
#10
Rachid Lefryekh, Ahmed Bensaad, Fatimazahra Bensardi, Khalid Elhattabi, Mounir Bouali, Bessam Daif, Abdelaziz Fadil, Zakaria Jaouhari, Tazi Hicham, Aziz Hamdani, Maha Soussi Abdalaoui
Fascioliasis is a zoonotic infection caused by a liver trematode: fasciola hepatica; which commonly affects cattle and sheep, humans are accidental hosts. Several cases have been reported in the literature worldwide with a large geographical distribution. We present a case of bile duct obstruction due to a hepatic fascioliasis, successfully treated with both a combined surgical and medical approaches. A high index of suspicion should be kept in mind for all cases of obstructive jaundice, especially in areas in which human fascioliasis infection is repeatedly reported...
2017: Pan African Medical Journal
https://www.readbyqxmd.com/read/29152559/fasciola-hepatica-esps-could-indistinctly-activate-or-block-multiple-toll-like-receptors-in-a-human-monocyte-cell-line
#11
Olgary Figueroa-Santiago, Ana M Espino
Fasciola hepatica is a parasitic helminth that induces Th2/Treg responses in its mammalian host. Some reports have suggested that ESPs achieve these polarized immune responses by delaying the activation of dendritic cells and macrophages during the early stages of innate immunity, a process that is mediated by TLR4. The present study aimed to investigate whether TLRs other than TLR4 could also be targeted by F. hepatica ESPs. To achieve this aim a screening system was optimized using THP1-Blue CD14 cells. ESPs were first separated based on their molecular weight and according their net charge by ion exchange chromatography (IEC)...
2017: Annals of Clinical Pathology
https://www.readbyqxmd.com/read/29151443/fasciola-hepatica-infection-in-water-buffalo-bubalus-bubalis-in-three-provinces-of-the-nile-delta-egypt-a-cross-sectional-study
#12
Abdelgawad Salah El-Tahawy, Nigel Kwan, Katsuaki Sugiura
A cross-sectional study was conducted to estimate the prevalence of Fasciola hepatica (F. hepatica) infection in water buffalo (Bubalus bubalis) in Alexandria, Beheira, and Kafr el-Sheikh governorates (provinces) of the Nile Delta in Egypt and to identify the underlying risk factors associated with the infection. A total of 29 farms (10 in Alexandria, 10 in Beheira, and 9 in Kafr el-Sheikh) were randomly selected and all the buffaloes that resided on these farms from 21 February 2015 to 20 February 2016 were included in the study...
November 20, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29143720/epidemiological-investigation-of-a-severe-rumen-fluke-outbreak-on-an-irish-dairy-farm
#13
James O'Shaughnessy, Andres Garcia-Campos, Conor G McAloon, Seamus Fagan, Theo de Waal, Maire McElroy, Micheal Casey, Barbara Good, Grace Mulcahy, John Fagan, Denise Murphy, Annetta Zintl
Although the rumen fluke, Calicophoron daubneyi is now very common and widespread throughout Western Europe, reports of clinical cases are still rare. This study explores the epidemiological background to a severe rumen fluke outbreak in 6-month-old heifers on a dairy farm in Ireland. Sequence analysis of the cytochrome oxidase subunit 1 (Cox1) gene of the rumen fluke metacercariae on pasture failed to identify predominant, possibly pathogenic subtypes. However, estimates of metacercarial load indicated that the animals were exposed to a daily dose of about 5334 C...
November 16, 2017: Parasitology
https://www.readbyqxmd.com/read/29132905/purification-and-biochemical-characterization-of-a-22-kda-stable-cysteine-like-protease-from-the-excretory-secretory-product-of-the-liver-fluke-fasciola-hepatica-by-using-conventional-techniques
#14
Ahmed Hemici, Roumaila Sabrina Benerbaiha, Dalila Bendjeddou
This study describes the purification and characterization of a stable protease activity isolated from Fasciola hepatica adult worms maintained in vitro by employing acetone precipitation (40-60%) followed by a gel filtration through Sephadex G-100 and DEAE- cellulose ion exchange column. Through this three-step purification, the enzyme was purified 11-fold with a specific activity of 1893.9U/mg and 31.5% recovery. After the final ultrafiltration step, the purification fold was increased up to 13.1 and the overall activity yield reached a rate of 18...
October 28, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29121986/farm-level-risk-factors-for-fasciola-hepatica-infection-in-danish-dairy-cattle-as-evaluated-by-two-diagnostic-methods
#15
Nao Takeuchi-Storm, Matthew Denwood, Tina Vicky Alstrup Hansen, Tariq Halasa, Erik Rattenborg, Jaap Boes, Heidi Larsen Enemark, Stig Milan Thamsborg
BACKGROUND: The prevalence of bovine fasciolosis in Denmark is increasing but appropriate guidelines for control are currently lacking. In order to help develop a control strategy for liver fluke, a risk factor study of farm management factors was conducted and the utility of bulk tank milk (BTM ELISA) as a tool for diagnosis in Danish dairy cattle farms was assessed. METHODS: This case-control study aimed to identify farm-level risk factors for fasciolosis in Danish dairy farms (> 50 animals slaughtered in 2013) using two diagnostic methods: recordings of liver condemnation at slaughter, and farm-level Fasciola hepatica antibody levels in BTM...
November 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/29118312/dynamics-of-liver-enzymes-in-rabbits-experimentally-infected-with-fasciola-sp-intermediate-form-from-japan
#16
Wipaporn Jarujareet, Kensuke Taira, Hong Kean Ooi
Dynamics of serum liver enzymes in rabbits experimentally infected with metacercariae of Fasciola sp. (intermediate form between Fasciola hepatica and F. gigantica) were monitored. Gradual increase of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were observed from 3 weeks post-inoculation (WPI) and peaked at 6 WPI, which corresponded well to the period of migration and development of juvenile fluke in the liver parenchyma and the time when the young adult flukes migrated to the bile duct...
November 9, 2017: Journal of Veterinary Medical Science
https://www.readbyqxmd.com/read/29111192/antifungal-molluscicidal-and-larvicidal-assessment-of-anemonin-and-clematis-flammula-l-extracts-against-mollusc-galba-truncatula-intermediate-host-of-fasciola-hepatica-in-tunisia
#17
Rakia Saidi, Lamia Khanous, Safa Khadim Allah, Besma Hamdi, Ali Ayadi, Mohamed Damak, Hayet Hammami, Raoudha Mezghani-Jarraya
OBJECTIVE: To investigate the potential of anemonin and Clematis flammula (C. flammula) extracts against infective organisms. METHODS: The molluscicidal activities of anemonin and C. flammula extracts against Galba truncatula Müll. (Lymnaeidae) and Fasciola hepatica larval stages contaminating this snail in Tunisia were assessed by testing six groups of snails in 250 mL of extracts and aqueous dechlorinated solutions with different concentrations (ranging from 2...
October 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/29027596/10-year-parasitological-examination-results-2003-to-2012-of-faecal-samples-from-horses-ruminants-pigs-dogs-cats-rabbits-and-hedgehogs
#18
Katharina Raue, Lea Heuer, Claudia Böhm, Sonja Wolken, Christian Epe, Christina Strube
The results of coproscopical examinations in domestic animals and hedgehogs carried out as routine diagnostics in the years 2003 to 2012 at the Institute for Parasitology, University of Veterinary Medicine Hannover, Germany, are presented. Of 3475 horse faecal samples, 30.1% contained stages of strongyles and 1.3% eggs of Strongyloides westeri and Parascaris equorum, respectively. The most frequently observed parasite stages in 1416 cattle faecal samples were Eimeria oocysts (21.3%) and strongyle eggs or larvae (15...
December 2017: Parasitology Research
https://www.readbyqxmd.com/read/29023077/spermatogenic-and-phylo-molecular-characterizations-of-isolated-fasciola-spp-from-cattle-north-west-iran
#19
Soheila Rouhani, Saber Raeghi, Adel Spotin
BACKGROUND AND OBJECTIVE: Fascioliasis is economically important to the livestock industry that caused with Fasciola hepatica and Fasciola gigantica. The objective of this study was to identify these two species F. hepatica and F. gigantica by using nuclear and mitochondrial markers (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola spp. MATERIALS AND METHODS: Approximately 150 Fasciola specimens were collected, then stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29016614/patterns-and-rates-of-abdominal-lymphatic-metastasis-following-esophageal-carcinoma
#20
Junqiang Chen, Wenjie Cai, Yu Lin, Yuanmei Chen, Qingfeng Zheng, Jianji Pan, Chuanben Chen
AIM: To determine the rate of abdominal lymph node metastasis after radical surgery for esophageal cancer and define the radiotherapy target area. METHODS: Of the 1593 patients who underwent R0 radical esophagectomy for thoracic esophageal squamous cell carcinoma (TE-SCC), 148 developed abdominal lymph node (LN) metastases within three years of surgery. During that time interval, patients were examined by various imaging methods (enhanced computer tomography, magnetic resonance imaging, and positron emission tomography-CT) at set time points...
2017: PloS One
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