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https://www.readbyqxmd.com/read/29027596/10-year-parasitological-examination-results-2003-to-2012-of-faecal-samples-from-horses-ruminants-pigs-dogs-cats-rabbits-and-hedgehogs
#1
Katharina Raue, Lea Heuer, Claudia Böhm, Sonja Wolken, Christian Epe, Christina Strube
The results of coproscopical examinations in domestic animals and hedgehogs carried out as routine diagnostics in the years 2003 to 2012 at the Institute for Parasitology, University of Veterinary Medicine Hannover, Germany, are presented. Of 3475 horse faecal samples, 30.1% contained stages of strongyles and 1.3% eggs of Strongyloides westeri and Parascaris equorum, respectively. The most frequently observed parasite stages in 1416 cattle faecal samples were Eimeria oocysts (21.3%) and strongyle eggs or larvae (15...
October 13, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29023077/spermatogenic-and-phylo-molecular-characterizations-of-isolated-fasciola-spp-from-cattle-north-west-iran
#2
Soheila Rouhani, Saber Raeghi, Adel Spotin
BACKGROUND AND OBJECTIVE: Fascioliasis is economically important to the livestock industry that caused with Fasciola hepatica and Fasciola gigantica. The objective of this study was to identify these two species F. hepatica and F. gigantica by using nuclear and mitochondrial markers (ITS1, ND1 and CO1) and have been employed to analyze intraspecific phylogenetic relations of Fasciola spp. MATERIALS AND METHODS: Approximately 150 Fasciola specimens were collected, then stained with haematoxylin-carmine dye and observed under an optical microscope to examine for the existence of sperm...
2017: Pakistan Journal of Biological Sciences: PJBS
https://www.readbyqxmd.com/read/29016614/patterns-and-rates-of-abdominal-lymphatic-metastasis-following-esophageal-carcinoma
#3
Junqiang Chen, Wenjie Cai, Yu Lin, Yuanmei Chen, Qingfeng Zheng, Jianji Pan, Chuanben Chen
AIM: To determine the rate of abdominal lymph node metastasis after radical surgery for esophageal cancer and define the radiotherapy target area. METHODS: Of the 1593 patients who underwent R0 radical esophagectomy for thoracic esophageal squamous cell carcinoma (TE-SCC), 148 developed abdominal lymph node (LN) metastases within three years of surgery. During that time interval, patients were examined by various imaging methods (enhanced computer tomography, magnetic resonance imaging, and positron emission tomography-CT) at set time points...
2017: PloS One
https://www.readbyqxmd.com/read/28993458/dairy-heifers-naturally-exposed-to-fasciola-hepatica-develop-a-type-2-immune-response-and-concomitant-suppression-of-leukocyte-proliferation
#4
John Graham-Brown, Catherine Hartley, Helen Clough, Aras Kadioglu, Matthew Baylis, Diana J L Williams
Fasciola hepatica is a parasitic trematode of global importance in livestock. Control strategies reliant on anthelmintics are unsustainable due to the emergence of drug resistance. Vaccines are under development, but efficacy is variable. Evidence from experimental infection suggest vaccine efficacy may be affected by parasite-induced immunomodulation. Little is known about the immune response to F. hepatica following natural exposure. Hence we analysed the immune responses over time in calves naturally exposed to F...
October 9, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28991277/centro-de-trasplante-hep%C3%A3-tico-en-m%C3%A3-xico-con-bajo-volumen-y-excelentes-resultados
#5
Mario Vilatobá, Miguel Ángel Mercado, Alan Gabriel Contreras-Saldivar, Rafael Paulino Leal-Villalpando, Jorge Zamudio-Bautista, Ignacio García-Juárez, Armando Gamboa-Domínguez
other: El trasplante hepático ortotópico (THO) es el tratamiento de elección para la insuficiencia hepática terminal. Numerosos estudios muestran una relación inversa entre el número de procedimientos y la mortalidad operatoria. El objetivo de este estudio es mostrar los resultados de nuestro centro y determinar si puede tener resultados equiparables a los obtenidos en centros de alto volumen. Es un estudio retrospectivo en el que se analizó la información de pacientes con THO en nuestra institución, de 1985 al 31 de diciembre de 2012...
2017: Revista de Investigación Clínica; Organo del Hospital de Enfermedades de la Nutrición
https://www.readbyqxmd.com/read/28984428/fasciola-and-fasciolosis-in-ruminants-in-europe-identifying-research-needs
#6
N J Beesley, C Caminade, J Charlier, R J Flynn, J E Hodgkinson, A Martinez-Moreno, M Martinez-Valladeres, J Perez, L Rinaldi, D J L Williams
Fasciola hepatica is a trematode parasite with a global distribution, which is responsible for considerable disease and production losses in a range of food producing species. It is also identified by WHO as a re-emerging neglected tropical disease associated with endemic and epidemic outbreaks of disease in human populations. In Europe, F. hepatica is mostly associated with disease in sheep, cattle and goats. This study reviews the most recent advances in our understanding of the transmission, diagnosis, epidemiology and the economic impact of fasciolosis...
October 6, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28973017/immunolocalization-and-immunodetection-of-the-excretory-secretory-es-antigens-of-fasciola-gigantica
#7
M A Hannan Khan, Rizwan Ullah, Abdur Rehman, Lubna Rehman, Ahammed Shareef P A, S M A Abidi
The digenetic trematode Fasciola gigantica is a parasite of great agricultural and economic importance. Along with Fasciola hepatica, F. gigantica incurs huge economic losses to the agricultural sector. Because of unavailability of an effective and commercial vaccine, the earliest diagnosis of the disease is the only way to control the disease. The conventional coprological techniques are able to detect the disease only after the parasites get matured and starts releasing their eggs with the faeces of host, therefore prepatent infection remain undiagnosed...
2017: PloS One
https://www.readbyqxmd.com/read/28969834/first-genetic-characterization-of-fasciola-hepatica-in-argentina-by-nuclear-and-mitochondrial-gene-markers
#8
Silvana Carnevale, Jorge Bruno Malandrini, María Laura Pantano, Claudia Cecilia Soria, Rosângela Rodrigues-Silva, José Roberto Machado-Silva, Jorge Néstor Velásquez, Laura Kamenetzky
Fasciola hepatica is a trematode showing genetic variation among isolates from different regions of the world. The objective of this work was to characterize for the first time F. hepatica isolates circulating in different regions of Argentina. Twenty-two adult flukes were collected from naturally infected bovine livers in different areas from Argentina and used for DNA extraction. We carried out PCR amplification and sequence analysis of the ribosomal internal transcribed spacer 1 (ITS1), mitochondrial nicotinamide adenine dinucleotide dehydrogenase subunits 4 and 5 (nad4 and nad5) and mitochondrial cytochrome c oxidase subunit I (cox1) genes as genetic markers...
October 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28969782/application-of-a-coproantigen-elisa-as-an-indicator-of-efficacy-against-multiple-life-stages-of-fasciola-hepatica-infections-in-sheep
#9
S D George, K Vanhoff, K Baker, L Lake, P F Rolfe, W Seewald, D L Emery
At present diagnosis of true resistance and determination of drug efficacy in Fasciola hepatica infection rely solely on terminal experiments. The coproantigen ELISA (cELISA) has been reported previously as a sensitive and specific tool appropriate to detect treatment failure, and potentially drug resistance. Two studies were conducted to determine whether the cELISA was appropriate for on-farm efficacy and resistance testing in Australian Merino sheep. In Study 1 sheep were infected orally with 50 F. hepatica metacercariae on three occasions, twelve, six and two weeks prior to a single flukicide treatment with triclabendazole, closantel or albendazole...
November 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28954086/hepatic-fascioliasis-in-mashhad-northeast-iran-first-report
#10
Alireza Badirzadeh, Sadaf Sabzevari
Fascioliasis is a zoonotic disease caused by a leaf-like worm (fluke) called Fasciola. Herein, we present a case of human hepatic fascioliasis. A 57-year-old man was referred to the hospital for ambiguous gastrointestinal symptoms with suspected hemangioma. Hepatic fascioliasis was diagnosed using abdominal computed tomography and serology. He tested positive for the IgG antibody against Fasciola hepatica. The patient was treated successfully with triclabendazole. This is the first published report on the occurrence of fascioliasis in Northeast Iran, a non-endemic area for fascioliasis...
July 2017: Revista da Sociedade Brasileira de Medicina Tropical
https://www.readbyqxmd.com/read/28936836/-molecular-cloning-and-characterization-of-two-kind-of-heat-shock-protein-gene-from-polyporus-umbellatus
#11
Meng-Meng Liu, Yong-Mei Xing, Shun-Xing Guo
With RT-PCR approaches, the full-length cDNA of two heat shock protein genes were cloned from total RNA of the Polyporus umbellatus sclerotium. The full open reading frame cDNA sequence of the Hsp90 was 2 091 bp, encoding 696 amino acid residues with a predicted molecular mass of 78.9 kDa. The full open reading frame cDNA sequence of the Hsp70 was 1 944 bp, encoding 647 amino acid residues with a predicted molecular mass of 70.5 kDa. The Hsp90 and Hsp70 protein contained the conservative structure domain, respectively...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28917540/galba-truncatula-and-fasciola-hepatica-genetic-costructures-and-interactions-with-intermediate-host-dispersal
#12
Ana C Correa, Thierry De Meeûs, Gilles Dreyfuss, Daniel Rondelaud, Sylvie Hurtrez-Boussès
Antagonistic interactions between hosts and parasites are key structuring forces in natural populations. Demographic factors like extinction, migration and the effective population size shape host-parasite metapopulational dynamics. Therefore, to understand the evolution of host-parasite systems it is necessary to study the distribution of the genetic variation of both entities simultaneously. In this paper, we investigate the population genetics co-structure of parasites and hosts within a metapopulation of the liver fluke, Fasciola hepatica, and two of its intermediate hosts, the main intermediate host in Europe, Galba truncatula, and a new intermediate host, Omphiscola glabra, in Central France...
September 14, 2017: Infection, Genetics and Evolution
https://www.readbyqxmd.com/read/28915255/scrambled-eggs-a-highly-sensitive-molecular-diagnostic-workflow-for-fasciola-species-specific-detection-from-faecal-samples
#13
Nichola Eliza Davies Calvani, Peter Andrew Windsor, Russell David Bush, Jan Šlapeta
BACKGROUND: Fasciolosis, due to Fasciola hepatica and Fasciola gigantica, is a re-emerging zoonotic parasitic disease of worldwide importance. Human and animal infections are commonly diagnosed by the traditional sedimentation and faecal egg-counting technique. However, this technique is time-consuming and prone to sensitivity errors when a large number of samples must be processed or if the operator lacks sufficient experience. Additionally, diagnosis can only be made once the 12-week pre-patent period has passed...
September 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28871261/the-chronic-stages-of-bovine-fasciola-hepatica-are-dominated-by-cd4-t-cell-exhaustion
#14
Divya Sachdev, Kevin C Gough, Robin J Flynn
Fasciola hepatica infection of ruminants leads to non-resolving chronic infection, as patency develops, there is switching to a TGF-β and IL-10 led response. Here, we explore the responses of CD4 T-cells within the major draining lymph nodes. We found minimal expression of Foxp3 within CD4 cells but elevated levels within the γδ (WC1(+)) population. There is a strong T-cell-intrinsic exhaustion phenotype within the hepatic lymph node (HLN) characterized by a lack of antigen-specific proliferation and cytokine secretion...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28870243/human-fascioliasis-by-fasciola-hepatica-the-first-case-report-in-nepal
#15
Ranjit Sah, Shusila Khadka, Mohan Khadka, Dipesh Gurubacharya, Jeevan Bahadur Sherchand, Keshab Parajuli, Niranjan Prasad Shah, Hari Prasad Kattel, Bharat Mani Pokharel, Basista Rijal
BACKGROUND: Fascioliasis is a zoonotic disease caused by Fasciola species. Patient may be asymptomatic or presents with jaundice and biliary colic or right hypochondriac pain due to bile duct obstruction with gastrointestinal symptoms. CASE PRESENTATION: We report a case of human fascioliasis in a 45 years old female presented to Tribhuvan University Teaching Hospital (TUTH), Kathmandu, Nepal on August, 2015 with fever, right hypochondriac pain, jaundice and occasional vomiting with anorexia for 4 months whose alkaline phosphatase was elevated and peripheral blood smear revealed eosinophilia...
September 5, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28843682/herd-level-prevalence-of-selected-endemic-infectious-diseases-of-dairy-cows-in-great-britain
#16
Martina Velasova, Angela Damaso, Bhagyalakshmi Chengat Prakashbabu, Jenny Gibbons, Nick Wheelhouse, David Longbottom, Steven Van Winden, Martin Green, Javier Guitian
To implement appropriate and effective disease control programs at the national level, up-to-date and unbiased information on disease frequency is needed. The aim of this study was to estimate the prevalence of selected endemic infectious diseases in the population of dairy herds in Great Britain. Bulk milk tank (BMT) samples from 225 randomly selected dairy farms, stratified by region and herd size, were tested for antibodies against bovine viral diarrhea virus (BVDV), bovine herpesvirus type 1, Mycobacterium avium ssp...
November 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28823007/excretory-secretory-products-from-two-fasciola-hepatica-isolates-induce-different-transcriptional-changes-and-il-10-release-in-lps-activated-bovine-boma-macrophages
#17
Piotr Bąska, Luke James Norbury, Anna Zawistowska-Deniziak, Marcin Wiśniewski, Kamil Januszkiewicz
Fasciola hepatica are trematodes that reside in the bile ducts of mammals. Infection causes US$3 billion in losses annually in animal production and is considered a zoonosis of growing importance. An under-represented area in F. hepatica research has been the examination of the different immunomodulatory abilities of various parasite isolates on the host immune system. In this paper, this issue was explored, with the bovine macrophage cell line "BOMA". The cells were matured by LPS treatment and stimulated with excretory/secretory antigens (ES) from two Fasciola hepatica isolates: a laboratory isolate "Weybridge" (Fh-WeyES) and a wild isolate (Fh-WildES)...
August 19, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28820707/prevalence-of-strongyloides-stercoralis-and-other-intestinal-parasite-infections-in-school-children-in-a-rural-area-of-angola-a-cross-sectional-study
#18
María Luisa Ruíz de Alegría Aznar, Karen Colmenares, Mateu Espasa, Arancha Amor, Isabel Lopez, Arlette Ninda, Joaquina Kanjala, Domingas Guilherme, Elena Sulleiro, Begoña Barriga, Eva Gil, Fernando Salvador, Cristina Bocanegra, Teresa López, Milagros Moreno, Israel Molina
Strongyloides stercoralis is widely distributed in the tropics and subtropics. The aim of this study was to determine the prevalence of S. stercoralis and other intestinal parasites and identify the risk factors for infection with S. stercoralis in a rural area of Angola. A cross-sectional study was conducted in school-age children (SAC) in Cubal, Angola. A questionnaire collecting clinical and epidemiological variables was used, and two stool samples were collected. A concentration technique (Ritchie) and a technique for detection of larvae migration (Baermann) were performed...
July 31, 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28815724/secreted-products-of-fasciola-hepatica-inhibit-the-induction-of-t-cell-responses-that-mediate-allergy
#19
C M Finlay, A M Stefanska, M M Coleman, H Jahns, J P Cassidy, R M McLoughlin, K H G Mills
There is evidence from epidemiology studies of a negative association between infection with helminth parasites and the development of allergy and asthma. Here, we demonstrate that the excretory/secretory products of the helminth Fasciola hepatica (FHES) protected mice against ovalbumin (OVA)-induced allergic asthma when administered at time of allergen sensitization. FHES reduced the accumulation of mucus, eosinophils and lymphocytes into the airways of allergen-challenged mice. Furthermore, FHES treatment suppressed Th2 responses in the airways...
August 16, 2017: Parasite Immunology
https://www.readbyqxmd.com/read/28807278/helminth-infections-on-organic-dairy-farms-in-spain
#20
I Orjales, M Mezo, M Miranda, M González-Warleta, F Rey-Crespo, M Vaarst, S Thamsborg, F J Diéguez, J A Castro-Hermida, M López-Alonso
The aim of this study was to assess the prevalence of the major helminth infections affecting organic dairy cattle in northern Spain. Milk and faecal samples were obtained from 443 milking cows. Ostertagia ostertargi and Fasciola hepatica exposure was assessed by detection of specific antibodies in milk samples and F. hepatica infection was diagnosed by the detection of coproantigens in faecal samples. Dictyocaulus viviparus and Calicophoron daubneyi infections were diagnosed by conventional coprological techniques...
August 30, 2017: Veterinary Parasitology
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