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https://www.readbyqxmd.com/read/29426482/comparison-of-early-detection-of-fasciola-hepatica-in-experimentally-infected-merino-sheep-by-real-time-pcr-coproantigen-elisa-and-sedimentation
#1
Nichola Eliza Davies Calvani, Sarah Deanna George, Peter Andrew Windsor, Russell David Bush, Jan Šlapeta
Fasciolosis due to infection with Fasciola hepatica, Fasciola gigantica or their hybrids is a significant global cause of livestock production loss. Infection is commonly diagnosed by a labour-intensive sedimentation and faecal egg count (FEC), which has limited throughput and is only applicable after completion of the 8-12 week pre-patent period (PPP). A commercially-available ELISA for the detection of coprological antigen (coproELISA) enables detection prior to the completion of the PPP and is suitable for diagnosis of larger sample sizes, although the sensitivity reported under experimental infection settings can be difficult to replicate in the field, particularly in cattle...
February 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29426464/a-new-multiplex-pcr-assay-to-distinguish-among-three-cryptic-galba-species-intermediate-hosts-of-fasciola-hepatica
#2
Pilar Alda, Manon Lounnas, Antonio Alejandro Vázquez, Rolando Ayaqui, Manuel Calvopiña, Maritza Celi-Erazo, Robert T Dillon, Philippe Jarne, Eric S Loker, Flavia Caroll Muñiz Pareja, Jenny Muzzio-Aroca, Alberto Orlando Nárvaez, Oscar Noya, Luiggi Martini Robles, Richar Rodríguez-Hidalgo, Nelson Uribe, Patrice David, Jean-Pierre Pointier, Sylvie Hurtrez-Boussès
A molecular tool described here allows in one step for specific discrimination among three cryptic freshwater snail species (genus Galba) involved in fasciolosis transmission, a worldwide infectious disease of humans and livestock. The multiplex PCR approach taken targets for each species a distinctive, known microsatellite locus which is amplified using specific primers designed to generate an amplicon of a distinctive size that can be readily separated from the amplicons of the other two species on an agarose gel...
February 15, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29414671/fibrinogen-and-fibrin-are-novel-substrates-for-fasciola-hepatica-cathepsin-l-peptidases
#3
Mirjam M Mebius, Jody M J Op Heij, Aloysius G M Tielens, Philip G de Groot, Rolf T Urbanus, Jaap J van Hellemond
Cathepsin peptidases form a major component of the secreted proteins of the blood-feeding trematodes Fasciola hepatica and Schistosoma mansoni. These peptidases fulfill many functions, from facilitating infection to feeding and immune evasion. In this study, we examined the Fasciola cathepsin L peptidases FhCL1, FhCL2, and FhCL3 and the schistosomal cathepsin peptidases SmCB1 and SmCL3 for their anticoagulant properties. Although no direct anticoagulant effect of these peptidases was observed, we discovered that cathepsin peptidases from Fasciola, but not from Schistosoma, were able to degrade purified fibrinogen, with FhCL1 having the highest fibrinogenolytic activity...
February 4, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/29409966/fasciola-hepatica-convoluted-life-forms-and-names
#4
Dieter Stürchler
No abstract text is available yet for this article.
January 30, 2018: Travel Medicine and Infectious Disease
https://www.readbyqxmd.com/read/29406086/co-infection-with-fasciola-hepatica-may-increase-the-risk-of-escherichia-coli-o157-shedding-in-british-cattle-destined-for-the-food-chain
#5
Alison K Howell, Sue C Tongue, Carol Currie, Judith Evans, Diana J L Williams, Tom N McNeilly
Escherichia coli O157 is a zoonotic bacterium that can cause haemorrhagic diarrhoea in humans and is of worldwide public health concern. Cattle are considered to be the main reservoir for human infection. Fasciola hepatica is a globally important parasite of ruminant livestock that is known to modulate its host's immune response and affect susceptibility to bacterial pathogens such as Salmonella Dublin. Shedding of E. coli O157 is triggered by unknown events, but the immune system is thought to play a part...
February 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29385479/genetic-correlations-between-endo-parasite-phenotypes-and-economically-important-traits-in-dairy-and-beef-cattle
#6
A J Twomey, R I Carroll, M L Doherty, N Byrne, D A Graham, R G Sayers, A Blom, D P Berry
Parasitic diseases have economic consequences in cattle production systems. Although breeding for parasite resistance can complement current control practices to reduce the prevalence globally, there is little knowledge of the implications of such a strategy on other performance traits. Records on individual animal antibody responses to Fasciola hepatica, Ostertagia ostertagi and Neospora caninum were available from cows in 68 dairy herds (study herds); national abattoir data on F. hepatica-damaged livers were also available from dairy and beef cattle...
January 29, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/29373193/antibody-recognition-of-cathepsin-l1-derived-peptides-in-fasciola-hepatica-infected-and-or-vaccinated-cattle-and-identification-of-protective-linear-b-cell-epitopes
#7
Laura Garza-Cuartero, Thomas Geurden, Suman M Mahan, John M Hardham, John P Dalton, Grace Mulcahy
Fasciola hepatica infection causes important economic losses in livestock and food industries around the world. In the Republic of Ireland F. hepatica infection has an 76% prevalence in cattle. Due to the increase of anti-helminthic resistance, a vaccine-based approach to control of Fasciolosis is urgently needed. A recombinant version of the cysteine protease cathepsin L1 (rmFhCL1) from F. hepatica has been a vaccine candidate for many years. We have found that vaccination of cattle with this immunodominant antigen has provided protection against infection in some experimental trials, but not in others...
January 17, 2018: Vaccine
https://www.readbyqxmd.com/read/29368659/pleiotropic-alterations-in-gene-expression-in-latin-american-fasciola-hepatica-isolates-with-different-susceptibility-to-drugs
#8
Santiago Radio, Santiago Fontenla, Victoria Solana, Anna C Matos Salim, Flávio Marcos Gomes Araújo, Pedro Ortiz, Cristian Hoban, Estefan Miranda, Valeria Gayo, Fabiano Sviatopolk-Mirsky Pais, Hugo Solana, Guilherme Oliveira, Pablo Smircich, José F Tort
BACKGROUND: Fasciola hepatica is the main agent of fasciolosis, a zoonotic disease affecting livestock worldwide, and an emerging food-borne disease in humans. Even when effective treatments are available, drugs are costly and can result in tolerance, liver damage and normally they do not prevent reinfection. Drug-resistant strains in livestock have been reported in various countries and, more worryingly, drug resistance in human cases has emerged in South America. The present study aims to characterize the transcriptome of two South American resistant isolates, the Cajamarca isolate from Peru, resistant to both triclabendazole and albendazole (TCBZR/ABZR) and the Rubino isolate from Uruguay, resistant to ABZ (TCBZS/ABZR), and compare them to a sensitive strain (Cenapa, Mexico, TCBZS/ABZS) to reveal putative molecular mechanisms leading to drug resistance...
January 24, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/29350372/endoscopic-diagnosis-and-treatment-of-biliary-obstruction-due-to-acute-cholangitis-and-acute-pancreatitis-secondary-to-fasciola-hepatica-infection
#9
Kemal Dolay, Mustafa Hasbahçeci, Engin Hatipoğlu, Fatma Ümit Malya, Adem Akçakaya
In the differential diagnosis of biliary obstruction with unknown etiology, biliary fascioliasis should be considered in endemic and nonendemic regions. After diagnostic evaluation, endoscopic retrograde cholangiopancreatography (ERCP) was performed for etiological evaluation and/or treatment of biliary obstruction in five patients with a mean age of 55.8 years. Endoscopic sphincterotomy and cholangiogram revealed linear filling defects in the biliary system. Fasciola hepatica parasites were extracted using balloon and basket catheters in two and three patients, respectively...
January 2018: Ulusal Travma Ve Acil Cerrahi Dergisi, Turkish Journal of Trauma & Emergency Surgery: TJTES
https://www.readbyqxmd.com/read/29346089/genererating-a-core-cluster-of-fasciola-hepatica-virulence-and-immunomodulation-related-genes-using-a-comparative-in-silico-approach
#10
Orçun Haçarız, Gearóid P Sayers
A total of 71 virulence and immunomodulation-related transcripts (VIRs) of Fasciola hepatica have been previously proposed (Haçarız et al., 2015). In an attempt to further refine this cohort, an in silico meta analysis approach was carried out using publicly available sequence data of related liver flukes, Clonorchis sinensis and Opisthorchis viverrini. Data of both liver flukes were investigated in terms of sequential homology with data of non-parasitic organisms, pathogens and VIRs of F. hepatica, directional selection (Ka/Ks), and cytokine signaling relation (protein motif based)...
December 28, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/29329624/apparent-prevalence-of-and-risk-factors-for-infection-with-ostertagia-ostertagi-fasciola-hepatica-and-dictyocaulus-viviparus-in-swiss-dairy-herds
#11
C F Frey, R Eicher, K Raue, C Strube, M Bodmer, B Hentrich, B Gottstein, N Marreros
Infections with helminth parasites can negatively affect performance of dairy cows. Knowledge on infection intensity, spatial distributions and risk factors are key to develop targeted treatment strategies. Canada and most EU countries have conducted large investigations, but respective data for Switzerland were missing. We now performed a bulk tank milk serosurvey for Ostertagia ostertagi, Fasciola hepatica, and Dictyocaulus viviparus on a total of 1036 voluntarily participating dairy herds that were sampled at confinement periods, i...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29329618/vaccination-against-fasciola-hepatica-using-cathepsin-l3-and-b3-proteases-delivered-alone-or-in-combination
#12
Agnieszka Wesołowska, Katarzyna Basałaj, Luke J Norbury, Alicja Sielicka, Halina Wędrychowicz, Anna Zawistowska-Deniziak
No licensed vaccine is currently available for prevention of Fasciola hepatica infections. However, considering the alarming increase in drug resistance, there is an urgent need for a safe and fully effective vaccine against fasciolosis. Here, we tested if cathepsins L (FhCL3-1, FhCL3-2) and B (FhCB3) secreted by juvenile liver flukes are viable vaccine targets when delivered alone or in combination in a rat model. Since control over the early immune response is crucial for parasite's establishment in its host, it was hypothesised that targeting fluke juvenile stages may prove beneficial...
January 30, 2018: Veterinary Parasitology
https://www.readbyqxmd.com/read/29321187/infection-by-the-helminth-parasite-fasciola-hepatica-requires-rapid-regulation-of-metabolic-virulence-and-invasive-factors-to-adjust-to-its-mammalian-host
#13
Krystyna Cwiklinski, Heather Jewhurst, Paul McVeigh, Tara Barbour, Aaron G Maule, Jose Tort, Sandra M O'Neill, Mark W Robinson, Sheila Donnelly, John P Dalton
The parasite Fasciola hepatica infects a broad range of mammals with impunity.  Following ingestion of parasites (metacercariae) by the host, newly excysted juveniles (NEJ) emerge from their cysts, rapidly penetrate the duodenal wall and migrate to the liver.  Successful infection takes just a few hours and involves negotiating hurdles presented by host macromolecules, tissues and micro-environments, as well as the immune system.  Here, transcriptome and proteome analysis of ex vivo F. hepatica metacercariae and NEJ reveal the rapidity and multitude of metabolic and developmental alterations that take place in order for the parasite to establish infection...
January 10, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29318989/study-on-the-relationship-between-liver-parasitic-infections-and-serum-vitamin-a-and-%C3%AE-carotene-status-in-cattle
#14
Ghader Jalilzadeh-Amin, Bijan Esmaeilnejad, Farhad Farhang-Pajuh
OBJECTIVE: This study was conducted to evaluate the relationship between serum levels of vitamin A and β-carotene in the liver of cattle. METHODS: A total number of 150 samples were selected according to the type of parasitic infections of the liver after postmortem examination and confirmation. Parasitic lesions in the liver were subdivided into three major parasites, including Fasciola hepatica, Dicrocoelium dendriticum, and hydatid cysts. Fifty samples were obtained from cattle without any parasitic infection as a control group...
December 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29318988/morphometric-and-molecular-study-of-fasciola-isolates-from-ruminants-in-iran
#15
Elham Akhlaghi, Mohammad Ali Mohammadi, Naser Ziaali, Mohammad Reza Baneshi, Saeid Nasibi, Hossein Kamyabi, Sima Rostami, Majid Fasihi Harandi
OBJECTIVE: The purpose of the present study was morphometric and molecular characterization of Fasciola isolates from ruminants in Iran. METHODS: Flukes were collected from the livers of 54 naturally infected sheep and cattle. The proportion of body length to width (L/W) of each fresh fluke was measured using a digital caliper. We employed receiver operating characteristic (ROC) curve analysis to explore the reliability of L/W for differentiating the two species...
December 2017: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/29284483/bovine-tuberculosis-visible-lesions-in-cattle-culled-during-herd-breakdowns-the-effects-of-individual-characteristics-trade-movement-and-co-infection
#16
Andrew W Byrne, Jordon Graham, Craig Brown, Aoibheann Donaghy, Maria Guelbenzu-Gonzalo, Jim McNair, Robin Skuce, Adrian Allen, Stanley McDowell
BACKGROUND: Bovine tuberculosis (bTB), caused by Mycobacterium bovis, remains a significant problem for livestock industries in many countries worldwide including Northern Ireland, where a test and slaughter regime has utilised the Single Intradermal Comparative Cervical Tuberculin (SICCT) test since 1959. We investigated the variation in post-mortem confirmation based on bTB visible lesion (VL) presence during herd breakdowns using two model suites. We investigated animal-level characteristics, while controlling for herd-level factors and clustering...
December 29, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29232690/rna-interference-in-fasciola-gigantica-establishing-and-optimization-of-experimental-rnai-in-the-newly-excysted-juveniles-of-the-fluke
#17
Arun Anandanarayanan, Opinder Krishen Raina, Hniang Lalrinkima, Ajayta Rialch, Muthu Sankar, Anju Varghese
Fasciolosis caused by Fasciola gigantica is a neglected tropical disease but a constraint on the growth and productivity of cattle, buffaloes and sheep in the tropical countries of Asia and Africa. Resistance to commonly used anthelmintics in Fasciola has increased the need to search for alternative therapeutic targets. RNA interference is the current tool of choice in the search for such targets in Fasciola. The susceptibility of juvenile Fasciola hepatica to double stranded (ds) RNA induced RNAi has been established but in F...
December 12, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/29221457/capillaria-hepatica-calodium-hepaticum-infection-in-a-horse-a-case-report
#18
Akihiro Ochi, Tatsuro Hifumi, Takanori Ueno, Yoshinari Katayama
BACKGROUND: Capillaria hepatica is a zoonotic parasite in humans and animals and has a worldwide distribution. However, infections in mammals apart from rodents, which are natural hosts of the parasite, have rarely been reported. This report describes the first known case of C. hepatica infection in a horse in Japan. CASE PRESENTATION: A 3-year-old filly without clinical signs was presented at a slaughterhouse in Japan. Gross examination revealed white to tan nodules 0...
December 8, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/29199372/comparative-assessment-of-recombinant-and-native-immunogenic-forms-of-fasciola-hepatica-proteins-for-serodiagnosis-of-sheep-fasciolosis
#19
Kobra Mokhtarian, Ahmad Reza Meamar, Majid Khoshmirsafa, Elham Razmjou, Leila Masoori, Majid Khanmohammadi, Lame Akhlaghi, Reza Falak
Laboratory diagnosis of sheep fasciolosis is commonly performed by coprological examinations; however, this method may lead to false negative results during the acute phase of the infection. Furthermore, the poor sensitivity of coprological methods is considered to be a paradox in the chronic phase of the infection. In this study, we compared the immunoreactivity of native and recombinant forms of Fasciola hepatica excretory/secretory antigens and determined their capabilities for the development of F. hepatica-specific immunoassays...
December 4, 2017: Parasitology Research
https://www.readbyqxmd.com/read/29187710/hybrid-origin-of-asian-aspermic-fasciola-flukes-is-confirmed-by-analyzing-two-single-copy-genes-pepck-and-pold
#20
Kei Hayashi, Madoka Ichikawa-Seki, Uday Kumar Mohanta, Takuya Shoriki, Pannigan Chaichanasak, Tadashi Itagaki
Nuclear gene markers, phosphoenolpyruvate carboxykinase (pepck) and DNA polymerase delta (pold), have been developed for precise discrimination of Fasciola flukes instead of internal transcribed spacer 1. In this study, these two genes of 730 Fasciola flukes from eight Asian countries were analyzed. The results were compared with their mitochondrial NADH dehydrogenase subunit 1 (nad1) lineages for obtaining a definitive evidence of the hybrid origin of aspermic Fasciola flukes. All the flukes categorized into the aspermic nad1 lineages possessed both the fragment patterns of F...
November 29, 2017: Journal of Veterinary Medical Science
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