keyword
MENU ▼
Read by QxMD icon Read
search

annexin A2

keyword
https://www.readbyqxmd.com/read/28211563/cytotoxic-analysis-and-chemical-characterization-of-fractions-of-the-hydroalcoholic-extract-of-the-euterpe-oleracea-mart-seed-in-the-mcf-7-cell-line
#1
Dayanne da S Freitas, José A Morgado-Díaz, Adriana S Gehren, Flávia C B Vidal, Raquel Maria T Fernandes, Wanderson Romão, Lilian V Tose, Fabiola N S Frazão, Maria Célia P Costa, Dulcelena F Silva, Maria do Desterro S B Nascimento
OBJECTIVES: To analyse the antineoplastic activity of fractions derived from the hydroalcoholic extract of Euterpe oleracea Mart. seed in the MCF-7 cell line and to identify the compounds responsible for the antineoplastic action. METHODS: Cells were treated with 10, 20, 40 and 60 μg/ml with the hexane, chloroform and ethyl acetate fraction (EAF) of the hydroalcoholic extract of açaí seed, for 24 and 48 h. After treatment, cell viability was measured using MTT assay and cell death was assessed using the Annexin-Pi assay...
February 17, 2017: Journal of Pharmacy and Pharmacology
https://www.readbyqxmd.com/read/28181408/epigallocatechin-gallate-induces-an-up-regulation-of-ldl-receptor-accompanied-by-a-reduction-of-pcsk9-via-the-annexin-a2-independent-pathway-in-hepg2-cells
#2
Kohei Kitamura, Yudai Okada, Kenji Okada, Yuya Kawaguchi, Satoshi Nagaoka
SCOPE: In animal studies, epigallocatechin gallate (EGCG), the dominant catechin in green tea, has been shown to improve cholesterol metabolism. However, the molecular mechanisms of EGCG underlying these functions have not been fully understood. In this study, we aimed to clarify the molecular mechanisms of the effect of EGCG on cholesterol metabolism mainly in HepG2 cells. METHODS AND RESULTS: We found that EGCG induced a reduction of the extracellular proprotein convertase subtilisin/kexin 9 (PCSK9) level accompanied by an up-regulation of the LDL receptor (LDLR) in HepG2 cells...
February 9, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28174683/post-translational-modifications-of-annexin-a2-are-linked-to-its-association-with-perinuclear-nonpolysomal-mrnp-complexes
#3
Ingvild Aukrust, Linn Andersen Rosenberg, Mia Madeleine Ankerud, Vibeke Bertelsen, Hanne Hollås, Jaakko Saraste, Ann Kari Grindheim, Anni Vedeler
Various post-translational modifications (PTMs) regulate the localisation and function of the multifunctional protein Annexin A2 (AnxA2). In addition to its various tasks as a cytoskeletal- and membrane-associated protein, AnxA2 can function as a trans-acting protein binding to cis-acting sequences of specific mRNAs. In the present study, we have examined the role of Ser25 phosphorylation in subcellular localisation of AnxA2 and its interaction with mRNP complexes. Subcellular fractionation and confocal microscopy of rat neuroendocrine PC12 cells showed that Ser25-phosphorylated AnxA2 (pSer25AnxA2) is absent from the nucleus and mainly localised to the perinuclear region, evidently associating with both membranes and cytoskeletal elements...
February 2017: FEBS Open Bio
https://www.readbyqxmd.com/read/28166601/mechanisms-of-cellular-activation-in-the-antiphospholipid-syndrome
#4
Nadine Müller-Calleja, Karl J Lackner
It is long known that antiphospholipid antibodies (aPL) induce proinflammatory and procoagulant cellular responses. The underlying signal transduction has been a major focus of research and is the topic of this review. An amazingly heterogeneous panel of signaling pathways has been described and it turns out that at least some of this heterogeneity can be explained by effects of distinct aPL species. On the one hand, there are antibodies against β2-glycoprotein I (β2GPI) which appear to exert their cellular effects only as a complex of β2GPI/anti-β2GPI...
February 6, 2017: Seminars in Thrombosis and Hemostasis
https://www.readbyqxmd.com/read/28152318/hedgehog-signaling-stimulates-tenascin-c-to-promote-invasion-of-pancreatic-ductal-adenocarcinoma-cells-through-annexin-a2
#5
Kelly Foley, Stephen Muth, Elizabeth Jaffee, Lei Zheng
Pancreatic adenocarcinoma (PDA) is characterized by a dense desmoplastic reaction that comprises 60-90% of the tumor, while only 10-40% of the tumor is composed of malignant epithelial cells. This desmoplastic reaction is composed of stromal fibroblast cells, extracellular matrix proteins, and immune cells. Accumulating evidence has suggested that the stromal and epithelial cell compartments interact during the pathogenesis of this disease. Therefore, it is important to identify the signaling pathways responsible for this interaction to better understand the mechanisms by which PDA invades and metastasizes...
February 2, 2017: Cell Adhesion & Migration
https://www.readbyqxmd.com/read/28130864/complex-roles-of-annexin-a2-in-host-blood-brain-barrier-invasion-by-cryptococcus-neoformans
#6
Wei Fang, Zhen-Zong Fa, Qun Xie, Gui-Zhen Wang, Jiu Yi, Chao Zhang, Guang-Xun Meng, Ju-Lin Gu, Wan-Qing Liao
INTRODUCTION: Fungal transversal across the brain microvascular endothelial cells (BMECs) is the essential step for the development of cryptococcal meningoencephalitis. Annexin A2 (AnxA2) is an important signaling protein involved in several intracellular processes such as membrane trafficking, endocytosis, and exocytosis. AIM: To investigate the roles and mechanism of AnxA2 during cryptococcal transversal of BMECs. RESULTS: Cryptococcus neoformans infection initiated upregulation of AnxA2 in mouse BMECs...
January 28, 2017: CNS Neuroscience & Therapeutics
https://www.readbyqxmd.com/read/28123570/role-of-annexin-a2-in-the-egf-induced-epithelial-mesenchymal-transition-in-human-caski-cells
#7
Lei Cui, Jian Song, Liting Wu, Luhui Cheng, Aijun Chen, Yanlin Wang, Yingdi Huang, Liming Huang
The epidermal growth factor receptor (EGF-R) signaling pathway is thought to have an important role in the development and progression of several carcinomas, as it is associated with cell proliferation, differentiation and migration. Activation of EGF-R signaling regulates epithelial-mesenchymal transition (EMT)-associated invasion and migration in normal and malignant epithelial cells. However, the specific mechanisms have not yet been fully elucidated. The present study utilized wound healing assays, western blotting, flow cytometry and MTT assays to demonstrate that Annexin A2 (ANXA2) is a key regulatory factor in EGF-induced EMT in CaSki cervical cancer cells...
January 2017: Oncology Letters
https://www.readbyqxmd.com/read/28088841/intracellular-fate-of-ureaplasma-parvum-entrapped-by-host-cellular-autophagy
#8
Fumiko Nishiumi, Michinaga Ogawa, Yukiko Nakura, Yusuke Hamada, Masahiro Nakayama, Jiro Mitobe, Atsushi Hiraide, Norio Sakai, Makoto Takeuchi, Tamotsu Yoshimori, Itaru Yanagihara
Genital mycoplasmas, including Ureaplasma spp., are among the smallest human pathogenic bacteria and are associated with preterm birth. Electron microscopic observation of U. parvum showed that these prokaryotes have a regular, spherical shape with a mean diameter of 146 nm. U. parvum was internalized into HeLa cells by clathrin-mediated endocytosis and survived for at least 14 days around the perinuclear region. Intracellular U. parvum reached endosomes in HeLa cells labeled with EEA1, Rab7, and LAMP-1 within 1 to 3 hr...
January 15, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28059611/molecular-characterization-of-pneumococcal-surface-protein-k-a-potential-pneumococcal-vaccine-antigen
#9
A-Yeung Jang, Ho Seong Seo, Shunmei Lim, Gook-Hyun Chung, Han Wool Kim, Sangyong Lim, Lei Zhao, In-Ho Park, Jae Hyang Lim, Kyung-Hyo Kim
The pneumococcal capsule is indispensable for pathogenesis in systemic infections; however, many pneumococcal diseases, including conjunctivitis, otitis media, and some systemic infections in immunocompromised patients, are caused by nonencapsulated Streptococcus pneumoniae (NESp). Null capsule clade 1 (NCC1), found in group 2 NESp, expresses pneumococcal surface protein K (PspK) and is becoming prevalent among pneumococcal organisms owing to the widespread use of pneumococcal conjugate vaccines. Despite its clinical importance, the molecular mechanisms underlying the prevalence of NCC1 have not been fully elucidated...
January 6, 2017: Virulence
https://www.readbyqxmd.com/read/27995049/annexin-a3-knockdown-suppresses-lung-adenocarcinoma
#10
Ying-Fu Liu, Qing-Qing Liu, Yue-Hua Zhang, Jing-Hua Qiu
Our previous study identified an elevated abundance of annexin A3 (Anxa3) as a novel prognostic biomarker of lung adenocarcinoma (LADC) through quantitative proteomics analysis. However, the biological functions of Anxa3 in LADC are not fully clear. In this study, in vitro and in vivo assays were performed to investigate the effects of Anxa3 downregulation on the growth, migration, invasion, metastasis, and signaling pathway activation of LADC cells. After Anxa3 downregulation, the growth of A549 and LTEP-a2 LADC cells was slowed and they showed decreased migration and invasion in vitro...
2016: Analytical Cellular Pathology (Amsterdam)
https://www.readbyqxmd.com/read/27974247/annexin-a2-is-critical-for-blood-testis-barrier-integrity-and-spermatid-disengagement-in-the-mammalian-testis
#11
Katarzyna Chojnacka, Barbara Bilinska, Dolores D Mruk
Throughout spermatogenesis, two important processes occur at late stage VIII of the seminiferous epithelial cycle in the rat testis: preleptotene spermatocytes commence entry into the adluminal compartment and step 19 spermatids release from the seminiferous epithelium. Presently, it is not clear how these processes, which involve extensive restructuring of unique Sertoli-Sertoli and Sertoli-germ cell junctions, are mediated. We aimed to determine whether annexin A2 (ANXA2), a Ca(2+)-dependent and phospholipid-binding protein, participates in cell junction dynamics...
December 11, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27956131/annexin-a2-is-involved-in-ca-2-dependent-plasma-membrane-repair-in-primary-human-endothelial-cells
#12
Sophia Nina Koerdt, Volker Gerke
Many cells in an organism are exposed to constant and acute mechanical stress that can induce plasma membrane injuries. These plasma membrane wounds have to be resealed rapidly to guarantee cell survival. Plasma membrane resealing in response to mechanical strain has been studied in some detail in muscle, where it is required for efficient recovery after insult. However, less is known about the capacity of other cell types and tissues to perform membrane repair and the underlying molecular mechanisms. Here we show that vascular endothelial cells, which are subject to profound mechanical burden, can reseal plasma membrane holes inflicted by laser ablation...
December 9, 2016: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27924845/caffeine-prevents-kidney-stone-formation-by-translocation-of-apical-surface-annexin-a1-crystal-binding-protein-into-cytoplasm-in-vitro-evidence
#13
Paleerath Peerapen, Visith Thongboonkerd
Recent large 3 cohorts have shown that caffeinated beverage consumption was associated with lower risk of kidney stone disease. However, its protective mechanisms remained unknown and had not been previously investigated. We thus evaluated protective effects of caffeine (1 μM-10 mM) on calcium oxalate monohydrate (COM) kidney stone formation, using crystallization, crystal growth, cell-crystal adhesion, Western blotting, and immunofluorescence assays. The results showed that caffeine reduced crystal number but, on the other hand, increased crystal size, resulting in unchanged crystal mass, consistent with crystal growth that was not affected by caffeine...
December 7, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27890659/rna-nanoparticles-harboring-annexin-a2-aptamer-can-target-ovarian-cancer-for-tumor-specific-doxorubicin-delivery
#14
Fengmei Pi, Hui Zhang, Hui Li, Varatharasa Thiviyanathan, David G Gorenstein, Anil K Sood, Peixuan Guo
A novel modified nucleic acid nanoparticle harboring an annexin A2 aptamer for ovarian cancer cell targeting and a GC rich sequence for doxorubicin loading is designed and constructed. The system utilizes a highly stable three-way junction (3WJ) motif from phi29 packaging RNA as a core structure. A phosphorothioate-modified DNA aptamer targeting annexin A2, Endo28, was conjugated to one arm of the 3WJ. The pRNA-3WJ motif retains correct folding of attached aptamer, keeping its functions intact. It is of significant utility for aptamer-mediated targeted delivery...
November 25, 2016: Nanomedicine: Nanotechnology, Biology, and Medicine
https://www.readbyqxmd.com/read/27889534/serum-annexin-a2-level-is-associated-with-diagnosis-and-prognosis-in-patients-with-oral-squamous-cell-carcinoma
#15
Wei Zhang, Chunhai Gao, Shaohua Zhang, Guiqing Fang
PURPOSE: In several human cancer types, serum annexin A2 levels are increased, but little is known regarding oral squamous cell carcinoma (OSCC). This study aimed to measure serum annexin A2 levels in OSCC patients and assess the association with diagnosis and prognosis. MATERIALS AND METHODS: This case-control study compared serum annexin A2 concentrations in a group of OSCC patients and a control group. The predictor variable was the presence or absence of OSCC, and the outcome variable was the level of serum annexin A2...
November 4, 2016: Journal of Oral and Maxillofacial Surgery
https://www.readbyqxmd.com/read/27871932/s100a10-regulates-ulk1-localization-to-er-mitochondria-contact-sites-in-ifn-%C3%AE-triggered-autophagy
#16
Ying-Da Chen, Yi-Ting Fang, Chih-Peng Chang, Chiou-Feng Lin, Li-Jin Hsu, Shang-Rung Wu, Yen-Chi Chiu, Robert Anderson, Yee-Shin Lin
During the process of autophagy, the autophagy-related proteins are translocated to autophagosome formation sites. Here, we demonstrate that S100A10 is required for ULK1 localization to autophagosome formation sites. Silencing of S100A10 reduces IFN-γ-induced autophagosome formation. We also determined the role of annexin A2 (ANXA2), a binding partner of S100A10, which has been reported to promote phagophore assembly. Silencing of ANXA2 reduced S100A10 expression. However, overexpression of S100A10 in ANXA2-silenced cells was still able to enhance autophagosome formation, suggesting that ANXA2 regulates IFN-γ-induced autophagy through S100A10...
January 6, 2017: Journal of Molecular Biology
https://www.readbyqxmd.com/read/27863502/annexin-a2-antibodies-but-not-inhibitors-of-the-annexin-a2-heterotetramer-impair-productive-hiv-1-infection-of-macrophages-in-vitro
#17
Andrew W Woodham, Adriana M Sanna, Julia R Taylor, Joseph G Skeate, Diane M Da Silva, Lodewijk V Dekker, W Martin Kast
During sexual transmission of human immunodeficiency virus (HIV), macrophages are initial targets for HIV infection. Secretory leukocyte protease inhibitor (SLPI) has been shown to protect against HIV infection of macrophages through interactions with annexin A2 (A2), which is found on the macrophage cell surface as a heterotetramer (A2t) consisting of A2 and S100A10. Therefore, we investigated potential protein-protein interactions between A2 and HIV-1 gp120 through a series of co-immunoprecipitation assays and a single molecule pulldown (SiMPull) technique...
November 18, 2016: Virology Journal
https://www.readbyqxmd.com/read/27836325/the-upregulation-of-annexin-a2-after-spinal-cord-injury-in-rats-may-have-implication-for-astrocyte-proliferation
#18
Jiajia Chen, Zhiming Cui, Saishuai Yang, Chunshuai Wu, Weidong Li, Guofeng Bao, Guanhua Xu, Yuyu Sun, Lingling Wang, Jinlong Zhang
Annexin A2 (ANXA2), is a member of the annexin family of proteins that exhibit Ca(2+)-dependent binding to phospholipids. One attractive biological function of ANXA2 is participating in DNA synthesis and cell proliferation. Previous studies have shown that ANXA2 play a role in the development of the central nervous system. However, the biological function of ANXA2 after spinal cord injury (SCI) is still with limited acquaintance. In the present study, we performed a SCI model in adult rats and investigated the dynamic changes of ANXA2 expression in the spinal cord...
February 2017: Neuropeptides
https://www.readbyqxmd.com/read/27821486/heterogeneous-stromal-signaling-within-the-tumor-microenvironment-controls-the-metastasis-of-pancreatic-cancer
#19
Agnieszka A Rucki, Kelly Foley, Pingbo Zhang, Qian Xiao, Jennifer Kleponis, Annie A Wu, Rajni Sharma, Guanglan Mo, Angen Liu, Jennifer Van Eyk, Elizabeth M Jaffee, Lei Zheng
Understanding how stromal signals regulate the development of pancreatic ductal adenocarcinoma (PDAC) may suggest novel therapeutic interventions in this disease. In this study, we assessed the metastatic role of stromal signals suggested to be important in the PDAC microenvironment. Src and IGF-1R phosphorylated the prometastatic molecule Annexin A2 (AnxA2) at Y23 and Y333 in response to stromal signals HGF and IGF-1, respectively, and IGF-1 expression was regulated by the Sonic Hedgehog (Shh) pathway. Both Shh and HGF were heterogeneously expressed in PDAC stroma, and only dual inhibition of these pathways could significantly suppress AnxA2 phosphorylation, PDAC growth, and metastasis...
January 1, 2017: Cancer Research
https://www.readbyqxmd.com/read/27819025/studies-on-shokyo-kanzo-and-keihi-in-kakkonto-medicine-on-prostaglandin-e2-production-in-lipopolysaccharide-treated-human-gingival-fibroblasts
#20
Toshiaki Ara, Norio Sogawa
We previously demonstrated that a kampo medicine, kakkonto, decreases lipopolysaccharide- (LPS-) induced prostaglandin E2 (PGE2) production by human gingival fibroblasts. In this study, we examined the herbs constituting kakkonto that exhibit this effect. Shokyo strongly and concentration dependently and kanzo and keihi moderately decreased LPS-induced PGE2 production. Shokyo did not alter cyclooxygenase-2 (COX-2) activity, cytosolic phospholipase A2 (cPLA2), annexin 1 and COX-2 expression, and LPS-induced extracellular signal-regulated kinase (ERK) phosphorylation...
2016: International Scholarly Research Notices
keyword
keyword
19200
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"