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Zhixu Chen, Zezheng Liu, Yang Yang, Zhaoyin Zhu, Ridong Liang, Bin Huang, Di Wu, Lei Yang, Hai Lu, Dadi Jin, Qingchu Li
Long non-coding RNAs (lncRNAs) have been shown to exert essential roles in development and progression of tumors. Here we discovered a novel lncRNA, RAB11B antisense RNA (RAB11B-AS1), which is markedly down-regulated in human osteosarcoma (OS) and associated with OS metastasis and poor prognosis. We find that reduction of RAB11B-AS1 significantly facilitates proliferation, migration and invasiveness and prevents apoptosis of OS cells and results in lower sensitivity to cisplatin in these cells. In contrast, up-regulation of RAB11B-AS1 suppresses the aggressive behaviors of OS cells...
June 1, 2018: Oncotarget
Bingxiu Zhou, Yuanyuan Yu, Lixia Yu, Binfu Que, Rui Qiu
Sipi soup (SPS), the aqueous extract derived from the root bark of Sophora japonical L, Salix babylonica L., Morus alba L., as well as Amygdalus davidiana (Carr.) C. de Vos, is a traditional Chinese medicine frequently used to prevent and treat infection and inflammation. However, the role of SPS in cancer‑associated fibroblasts (CAFs) require further investigation. In the present study, the effects of SPS on fibroblast inactivation and the underlying mechanism were investigated. Reverse transcription‑quantitative polymerase chain reaction was used to analyze the mRNA expression levels of fibroblast activation protein (FAP), interleukin (IL)‑6, α‑smooth muscle actin (α‑SMA) and programmed cell death 4 (PDCD4)...
June 6, 2018: Molecular Medicine Reports
Zhigang Qu, Zhe Quan, Qi Zhang, Zhenyu Wang, Qingxu Song, Xinming Zhuang, Changfeng Fu, Feng Xu, Yadong Liu, Yuanyi Wang, Zheng Wang, Yi Liu
The present study aimed to identify novel intervertebral disc degeneration (IDD)‑associated long noncoding (lnc)RNAs and genes. The lncRNA and mRNA microarray dataset GSE56081 was downloaded from the Gene Expression Omnibus database and included 5 samples from patients with degenerative lumbar nucleus pulposus and 5 normal controls. Differentially expressed lncRNAs or differentially expressed genes (DEGs) were identified and co‑expression network analysis was performed followed by functional analysis for genes in the network...
June 5, 2018: Molecular Medicine Reports
Wenjia Chai, Jing Li, Qilin Shangguan, Qi Liu, Xinda Li, Dandan Qi, Xiaomei Tong, Wenjun Liu, Xin Ye
LncRNAs are involved in many aspects of cellular processes, including antiviral immune response. To identify influenza A virus (IAV)-related lncRNAs, we performed RNA-deep sequencing to compare the profiles of lncRNAs in A549 and 293T cells with or without IAV infection. We identified an IAV-upregulated lncRNA named lnc-ISG20 because it shares most of its sequence with ISG20. We found that lnc-ISG20 is an interferon-stimulated gene similar to ISG20. Overexpression of lnc-ISG20 inhibited IAV replication, while lnc-ISG20 knockdown favored viral replication, suggesting that ln-ISG20 is inhibitory to IAV replication...
June 13, 2018: Journal of Virology
Nana Ma, Shaoru Li, Quanhua Zhang, Hongmei Wang, Haixia Qin, Shijin Wang
In recent decades, numerous long non-coding (lnc)RNAs, including growth arrest-specific transcript 5 (GAS5), have been demonstrated to exert promoting or suppressive effects in human cancers. Decreased expression of the lncRNA GAS5 was reported to promote cell proliferation, migration and invasion and indicate poor prognosis in ovarian cancer. However, the exact underlying molecular mechanism through which GAS5 is involved in ovarian cancer growth remains unknown. The present study aimed to investigate the regulatory mechanism of GAS5 in ovarian cancer cell proliferation...
July 2018: Experimental and Therapeutic Medicine
Kaiyuan Ji, Ruiwen Fan, Junzhen Zhang, Shanshan Yang, Changsheng Dong
To elucidate the Cdk5 regulatory molecular mechanism in skin, we generated Cdk5-knockdown mice and subjected their skins to lncRNA sequencing. The results showed that there were 4533 novel lncRNAs from 142 lncRNA families. In total, 693 lncRNAs were significantly differentially expressed. Alignment analysis of the lncRNAs in miRBase identified 45 pre-mRNAs. By KEGG PATHWAY Database analysis, we found that lncRNAs (lnc-NONMMUT064276.2, lnc-NONMMUT075728.1, and lnc-NONMMUT039653.2) may regulate pigmentation by regulating target genes...
June 8, 2018: Gene
Mariam Lofty Khaled, Yelena Bykhovskaya, Sarah E R Yablonski, Hanzhou Li, Michelle D Drewry, Inas F Aboobakar, Amy Estes, X Raymond Gao, W Daniel Stamer, Hongyan Xu, R Rand Allingham, Michael A Hauser, Yaron S Rabinowitz, Yutao Liu
Purpose: Keratoconus (KC) is the most common corneal ectasia. We aimed to determine the differential expression of coding and long noncoding RNAs (lncRNAs) in human corneas affected with KC. Methods: From the corneas of 10 KC patients and 8 non-KC healthy controls, 200 ng total RNA was used to prepare sequencing libraries with the SMARTer Stranded RNA-Seq kit after ribosomal RNA depletion, followed by paired-end 50-bp sequencing with Illumina Sequencer. Differential analysis was done using TopHat/Cufflinks with a gene file from Ensembl and a lncRNA file from NONCODE...
June 1, 2018: Investigative Ophthalmology & Visual Science
Jingjing Li, Jing Zhang, Liang Jin, Heran Deng, Jiannan Wu
BACKGROUND/AIM: Persistent activation of the phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin (mTOR) pathway is an important mechanism in resistance of breast cancer to endocrine therapy. Although everolimus has potent inhibitory effects on the mTOR pathway, it has demonstrated only modest clinical activity as a single agent. Whether long noncoding (lnc) RNA is involved in everolimus resistance is unknown. MATERIALS AND METHODS: Cell viability, colony formation and cell proliferation experiments were used to measure the effects of long noncoding RNA N-acylsphingosine amidohydrolase 2B-2 (lnc-ASAH2B-2) knockdown in BT474 and MCF7 breast cancer cells...
June 2018: Anticancer Research
Gang Qu, Zhiqiang Ma, Wenxian Tong, Jiahui Yang
Recently, numerous long non‑coding (lnc)RNAs have been revealed as serving important roles in human gene regulation. Previous studies have suggested that aberrant expression of lncRNAs is associated with cancer progression and metastasis. Previous studies have also demonstrated that decreased expression of WW domain‑containing oxidoreductase (WWOX) is associated with poor prognosis in numerous cancer types. However, the effect of WWOX antisense RNA 1 (WWOX‑AS1) in the development of cancer remains unknown...
May 23, 2018: Molecular Medicine Reports
Xiao Li, Qi Zhao, Jianni Qi, Wenwen Wang, Di Zhang, Zhen Li, Chengyong Qin
Aerobic glycolysis is a phenomenon by which malignant cells preferentially metabolize glucose through the glycolytic pathway, rather than oxidative phosphorylation to proliferate efficiently. The present study aimed to investigate the expression and functional implications of long non-coding (lnc)RNA Ftx in the aerobic glycolysis and tumorigenesis of hepatocellular carcinoma (HCC). It was identified that lncRNA Ftx was upregulated in human HCC tissues and cell lines and, notably, was associated with aggressive clinicopathological features...
May 23, 2018: International Journal of Oncology
Feng Guo, Zhili Cao, Huiqin Guo, Shanqing Li
The action mechanism of long non-coding ribonucleic acid-homeobox transcript antisense ribonucleic acid (lncRNA-HOTAIR) in the regulation of the Wnt signaling pathway on the drug resistance of non-small cell lung cancer was investigated. Forty eight patients with non-small cell lung cancer, who were treated with cisplatin (DDP) as neoadjuvant chemotherapy, were selected from the specimen bank of the Department of Pathology of Peking Union Medical College Hospital. Reverse transcription-polymerase chain reaction (RT-PCR) was used to detect the messenger RNA (mRNA) level of lncRNA-HOTAIR in cancer and cancer-adjacent tissues...
June 2018: Experimental and Therapeutic Medicine
Ningning Liu, Qi Liu, Xiaohai Yang, Fang Zhang, Xinda Li, Yuanwu Ma, Feifei Guan, Xin Zhao, Zhiwei Li, Lianfeng Zhang, Xin Ye
Recent studies have indicated that a number of lncRNAs are dysregulated in hepatocellular carcinoma, while their aberrant expressions are associated with tumorigenesis and poor prognosis. To identify HBV-related lncRNAs, we used RNA deep sequencing to quantify the abundances of lncRNAs in HepG2 cells and HBV transgenic HepG2-4D14 cells. Here, we demonstrate that lnc-HUR1 is significantly upregulated in HepG2-4D14 cells. We found that HBV-encoded HBx can enhance the transcription of lnc-HUR1. Overexpression of lnc-HUR1 promotes cell proliferation, while knockdown of lnc-HUR1 inhibits cell growth...
May 23, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
Karolina Elżbieta Kaczor-Urbanowicz, Harsh M Trivedi, Patricia O Lima, Paulo M Camargo, William V Giannobile, Tristan R Grogan, Frederico O Gleber-Netto, Yair Whiteman, Feng Li, Hyo Jung Lee, Karan Dharia, Katri Aro, Carmen Martin Carerras-Presas, Saarah Amuthan, Manjiri Vartak, David Akin, Hiba Al-Adbullah, Kanika Bembey, Perry R Klokkevold, David Elashoff, Virginia Monsul Barnes, Rose Richter, William DeVizio, James G Masters, David Wong
AIM: This study tests the hypothesis that salivary extracellular RNA (exRNA) biomarkers can be developed for gingivitis detection and monitoring disease regression. MATERIALS AND METHODS: Salivary exRNA biomarker candidates were developed from a total of 100 gingivitis and non-gingivitis individuals using Affymetrix's expression microarrays. The top ten differentially expressed exRNAs were tested in a clinical cohort to determine if the discovered salivary exRNA markers for gingivitis were associated with clinical gingivitis and disease regression...
May 19, 2018: Journal of Clinical Periodontology
Ziye Li, Lin Yang, Xiaojun Liu, Ziyuan Nie, Jianmin Luo
The long noncoding RNA (lnc) maternally expressed 3 (MEG3) is downregulated in many types of cancers. However, the relationship between lncRNA MEG3, microRNA-21 (miR-21) and chronic myeloid leukemia (CML) blast crisis is unknown. This study examined bone marrow samples from 40 CML patients and 10 healthy donors. Proliferation and apoptosis assays, real-time polymerase chain reaction (PCR), bisulfite sequencing PCR, Western blotting, luciferase assay, RNA pull-down, RNA immunoprecipitation (RIP), co-immunoprecipitation (CoIP) and Chromatin immunoprecipitation (ChIP) were performed...
May 14, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Mingtian Deng, Yongjie Wan, Zifei Liu, Caifang Ren, Guomin Zhang, Jing Pang, Yanli Zhang, Feng Wang
In mammals, their proper development during the early cleavage stages strongly relies on the gene products newly transcribed by zygotic genome activation (ZGA). Long noncoding RNAs (lncRNAs) have been characterized as key regulators of the ZGA process in mice and human. However, the ZGA stage has not yet been identified and epigenetic regulations of the ZGA process remain largely unknown in goats. Here, we show that ZGA occurred at the 8-cell stage in goats. During ZGA, tri-methylation of H3K9 was dynamically changed but maintained strong staining in development arrested embryos...
May 15, 2018: Biology of Reproduction
Zhengjun Liu, Guangyu Wu, Cai Lin, Hailei Guo, Jianjun Xu, Tianlan Zhao
The present study examined expression and potential functions of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) in human skin squamous cell carcinoma (SCC). We show that IGF2BP1 mRNA and protein expression levels were upregulated in established (A431 line) and primary human skin SCC cells. Its expression was also increased in human skin SCC tissues, as compared to the normal skin tissues. In skin SCC cells, IGF2BP1 silencing or CRISPR/Cas9 knockout decreased levels of IGF2BP1-stablized mRNAs, including IGF2, CD44, Gli1 and Myc...
May 14, 2018: Biochemical and Biophysical Research Communications
Hong Wu, Fang Wen, Mei Jiang, Qiang Liu, Yijun Nie
High glucose combined with high FFAs can contribute to the unfavorable development of type 2 diabetes mellitus (T2DM) and monocytes/macrophages are important in the occurrence and development of T2DM, which is regarded as a type of low‑grade inflammation. Although our previous study demonstrated that increased expression of P2X7 receptor (P2X7R) in peripheral blood monocytes may alter the innate immune system and that long non‑coding (lnc)RNA uc.48+ was involved in diabetic neuropathic pain, the involvement of uc...
May 9, 2018: International Journal of Molecular Medicine
Lei Huang, Lihua Zeng, Jiahui Chu, Pengfei Xu, Mingming Lv, Juan Xu, Juan Wen, Wenqu Li, Luyu Wang, Xiaowei Wu, Ziyi Fu, Hui Xie, Shui Wang
Breast cancer is the most commonly diagnosed cancer and the leading cause of cancer death in females worldwide. Chemoresistance has been a major reason for the drug therapy failure. The present study performed a microarray analysis between MCF‑7 and MCF‑7/adriamycin (ADR) cells, and intended to identify long non‑coding (lnc)RNA expression character in drug resistant breast cancer cells. MCF‑7/ADR cells were induced from MCF‑7 cells via pulse‑selection with doxorubicin for 4 weeks, and the resistance to doxorubicin of ADR cells was confirmed by MTT assay...
April 27, 2018: Molecular Medicine Reports
Farah Rahmatpanah, Sudhanshu Agrawal, Vanessa M Scarfone, Sameer Kapadia, Dan Mercola, Anshu Agrawal
Immune dysfunction is a hallmark of aging and is thought to be responsible for the age-associated diseases. Dendritic cells(DCs) of the immune system function as initiators and regulators of the immune responses. Recent studies have highlighted the division of labor between various DC subsets. CD1c+ DC subset has emerged as a major inducer of CD4 T cell response. There is a scarcity of information regarding the age-associated changes in the functions of DC subsets in the elderly. Here we investigated the changes in transcriptional profile of CD1c+ DC subset from healthy aged and young individuals using RNA-sequencing...
April 28, 2018: Journals of Gerontology. Series A, Biological Sciences and Medical Sciences
Jie Ji, Yin Yin, Huanyu Ju, Xiaoliang Xu, Wei Liu, Qiang Fu, Jiaojiao Hu, Xudong Zhang, Beicheng Sun
Although one of the first comprehensive examinations of long non-coding RNA (lncRNA) expression was performed in human CD8 T lymphocytes, little is known about their roles in CD8 T cells functions during the progression of hepatocellular carcinoma (HCC). Here, we show that Lnc-Tim3 is upregulated and negatively correlates with IFN-γ and IL-2 production in tumor-infiltrating CD8 T cells of HCC patients. Lnc-Tim3 plays a pivotal role in stimulating CD8 T exhaustion and the survival of the exhausted CD8 T cells...
April 30, 2018: Cell Death & Disease
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