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Lnc RNA

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https://www.readbyqxmd.com/read/29644261/detection-and-analysis-of-circular-rnas-by-rt-pcr
#1
Amaresh C Panda, Myriam Gorospe
Gene expression in eukaryotic cells is tightly regulated at the transcriptional and posttranscriptional levels. Posttranscriptional processes, including pre-mRNA splicing, mRNA export, mRNA turnover, and mRNA translation, are controlled by RNA-binding proteins (RBPs) and noncoding (nc)RNAs. The vast family of ncRNAs comprises diverse regulatory RNAs, such as microRNAs and long noncoding (lnc)RNAs, but also the poorly explored class of circular (circ)RNAs. Although first discovered more than three decades ago by electron microscopy, only the advent of high-throughput RNA-sequencing (RNA-seq) and the development of innovative bioinformatic pipelines have begun to allow the systematic identification of circRNAs (Szabo and Salzman, 2016; Panda et al ...
March 20, 2018: Bio-protocol
https://www.readbyqxmd.com/read/29643944/competing-endogenous-rna-expression-profiling-in-pre-eclampsia-identifies-hsa_circ_0036877-as-a-potential-novel-blood-biomarker-for-early-pre-eclampsia
#2
Xiaopeng Hu, Junping Ao, Xinyue Li, Huijuan Zhang, Ji Wu, Weiwei Cheng
Background: The etiology and pathogenesis of pre-eclampsia (PE) is unclear, and there is no ideal early clinical biomarker for prediction of PE. The competing endogenous RNA (ceRNA) hypothesis is a new approach to uncover the molecular pathology of PE. The first aim of this study was to perform messenger RNA, long non-coding RNA, and circular RNA (circRNA) expression profiling of human normal and severe pre-eclampsia (SPE) placentas. circRNA, which has a stable structure, is a more suitable biomarker than other types of RNA...
2018: Clinical Epigenetics
https://www.readbyqxmd.com/read/29626476/long-non-coding-rna-thor-promotes-human-osteosarcoma-cell-growth-in-vitro-and-in-vivo
#3
Wangzhen Chen, Meikai Chen, Yifan Xu, Xuerong Chen, Ping Zhou, Xiaofeng Zhao, Fei Pang, Wenqing Liang
Long non-coding RNA (LncRNA) dysregulation is associated with human osteosarcoma (OS) cell progression. Recent studies have characterized a novel but ultra-conserved LncRNA THOR ("Lnc-THOR") as a cancer-specific LncRNA, mediating cell growth. In the current study, we show that Lnc-THOR is expressed in established and primary human OS cells. It is also detected in human OS tissues, but not in the surrounding normal bone tissues. siRNA-induced knockdown or CRSIPR/Cas9-mediated knockout Lnc-THOR significantly inhibited human OS cell survival and proliferation...
April 4, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29626186/de-novo-reconstruction-of-human-adipose-transcriptome-reveals-conserved-lncrnas-as-regulators-of-brown-adipogenesis
#4
Chunming Ding, Yen Ching Lim, Sook Yoong Chia, Arcinas Camille Esther Walet, Shaohai Xu, Kinyui Alice Lo, Yanling Zhao, Dewen Zhu, Zhihui Shan, Qingfeng Chen, Melvin Khee-Shing Leow, Dan Xu, Lei Sun
Obesity has emerged as an alarming health crisis due to its association with metabolic risk factors such as diabetes, dyslipidemia, and hypertension. Recent work has demonstrated the multifaceted roles of lncRNAs in regulating mouse adipose development, but their implication in human adipocytes remains largely unknown. Here we present a catalog of 3149 adipose active lncRNAs, of which 909 are specifically detected in brown adipose tissue (BAT) by performing deep RNA-seq on adult subcutaneous, omental white adipose tissue and fetal BATs...
April 6, 2018: Nature Communications
https://www.readbyqxmd.com/read/29621586/long-non-coding-rna-dilc-suppresses-cell-proliferation-and-metastasis-in-colorectal-cancer
#5
Li-Qiang Gu, Xiang-Lei Xing, Hui Cai, An-Feng Si, Xian-Rong Hu, Qian-Yun Ma, Meng-Lin Zheng, Ruo-Yu Wang, Heng-Yu Li, Xi-Peng Zhang
Colorectal cancer (CRC) is one of the most common malignant tumors and one of the leading causes of cancer-related death in both men and women. The prognosis of CRC remains poor due to the advanced stage and cancer metastasis at the time of diagnosis. However, the exact mechanism of tumorigenesis in CRC remains unclear. Long non-coding RNAs (lncRNAs), which refer to transcripts longer than 200 nucleotides that are not translated into protein, are known to play important roles in multiple human cancers. Lnc-DILC is reported to be an important tumor suppressor gene and its inactivation is closely associated with liver cancer stem cells...
April 2, 2018: Gene
https://www.readbyqxmd.com/read/29614625/inhibition-of-e-cadherin-expression-by-lnc-rna-h19-to-facilitate-bladder-cancer-metastasis
#6
Zunwei Zhu, Lieyu Xu, Yong Wan, Jie Zhou, Donghui Fu, Haichao Chao, Kunwang Bao, Tao Zeng
Bladder cancer is derived from bladder mucosa and is the most common malignant tumor in urinary system. Long non-coding RNA (lnc-RNA) has high tissue specificity and can participate in cell proliferation and differentiation at various levels, and plays critical roles in occurrence of malignant tumors. This study aims to investigate the suppression of E-cadherin expression by lnc-RNA H19 to enhance bladder cancer metastasis.qRT-PCR was applied to analyze differential expression of lnc-RNA H19 in different bladder cancer tissues and tumor adjacent tissues...
March 19, 2018: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29576888/long-non-coding-rna-in-liver-metabolism-and-disease-current-status
#7
Yulan Zhao, Jianguo Wu, Suthat Liangpunsakul, Li Wang
Long non-coding RNAs (lncRNAs) are comprised of RNA transcripts exceeding 200 nucleotides in length but lacking identifiable open reading frames (with rare exceptions). Herein, we highlight emerging evidence demonstrating that lncRNAs are critical regulators of liver metabolic function and diseases. We summarize current knowledges about dysregulated lncRNAs and outline the underlying molecular mechanisms by which lncRNAs control hepatic lipid ad glucose metabolism, as well as cholestatic liver disease. lncLSTR, Lnc18q22...
September 2017: Liver Research
https://www.readbyqxmd.com/read/29552776/long-noncoding-rna-linc-ubc1-promotes-tumor-invasion-and-metastasis-by-regulating-ezh2-and-repressing-e-cadherin-in-esophageal-squamous-cell-carcinoma
#8
Guangcai Niu, Huijie Zhuang, Bingqiang Li, Gang Cao
PURPOSE: Recent reports have shown that long noncoding (lnc) RNAs are critical during tumorigenesis. This study focused on the influence of linc-UBC1 on the metastasis of esophageal squamous cell carcinoma (ESCC) and the underlying mechanism. METHODS: In 50 ESCC tissues and 5 ESCC cell lines, linc- UBC1 expression was detected by RT-qPCR. Moreover, correlation analysis was conducted between linc-UBC1 expression level and clinicopathological features. Overall survival of these ESCC patients was analyzed by Kaplan-Meier method...
January 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29552759/long-non-coding-rna-pvt1-functions-as-an-oncogene-in-human-colon-cancer-through-mir-30d-5p-runx2-axis
#9
Xiuguo Yu, Jianpei Zhao, Yaguang He
PURPOSE: Recently, long noncoding RNAs (lncRNAs) have caught more attention for their role in tumor progression. Colon cancer is one of these ordinary malignant tumors. This study aimed to identify how lnc RNA PVT1 affects the progression of colon cancer. METHODS: PVT1 expression of both colon cancer cell tissue and 60 paired cancer and peri-tumoral tissue samples was detected by real-time quantitative polymerase chain reaction (RT-qPCR). The associations between lnc RNA PVT1 expression level and clinicopathological characteristics and patients' disease-free survival rate were evaluated...
January 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29552610/data-on-long-noncoding-rna-upregulated-in-hypothermia-treated-cardiomyocytes-protects-against-myocardial-infarction-through-improving-mitochondrial-function
#10
Jian Zhang, Liming Yu, Yinli Xu, Yu Liu, Zhi Li, Xiaodong Xue, Song Wan, Huishan Wang
This article elaborates on cardioprotective action of hypothermia related long noncoding RNA against myocardial infarction through improving mitochondrial function, which preset by J Zhang. Herein, we provide the materials and methods used in that study. And provided the detail of dysregulation of lncRNAs under the treatment of hypothermia. Furthermore, we found that lnc-UIHTC (lncRNA upregulated in hypothermia treated cardiomyocyte, NONHSAT094064) attenuated cardiomyocytes apoptosis in vitro.
April 2018: Data in Brief
https://www.readbyqxmd.com/read/29543785/long-noncoding-rna-taurine-upregulated-gene1-tug1-promotes-tumor-growth-and-metastasis-through-tug1-mir-129-5p-astrocyte-elevated-gene-1-aeg-1-axis-in-malignant-melanoma
#11
Jianwen Long, Qiqige Menggen, Qimige Wuren, Quan Shi, Xianming Pi
BACKGROUND Malignant melanoma is a class of malignant tumors derived from melanocytes. lncRNAs have been considered as pro-/anti-tumor factors in progression of cancers. The function of lncRNA TUG1 on growth of melanoma was investigated in this study. MATERIAL AND METHODS The TUG1 and miR-129-5p expression were examined via qRT-PCR. The protein expression was investigated by Western blotting assay. Luciferase reporter assay was used to assess if lncRNA TUG1 can bind to miR-129-5p and if miR-129-5p can target AEG1 mRNA...
March 15, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29541223/long-non-coding-rna-hnf1a-as1-promotes-cell-viability-and-migration-in-human-bladder-cancer
#12
Zhihong Feng, Baolong Wang
Bladder cancer is among the most frequent types of genitourinary malignancies and results in high morbidity and mortality. Despite considerable progress in methods of bladder cancer diagnosis and treatment, the detailed underlying molecular mechanisms of bladder cancer remain unclear, and the prognosis of patients remains poor. In the present study, the role of long non-coding (lnc)RNA hepatocyte nuclear factor 1A (HNF1A)-antisense RNA (AS)1 in bladder cancer progression was examined in vitro . HNF1A-AS1 was overexpressed in clinical bladder cancer tissues and cultured bladder cancer cells...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29541183/linc00152-is-a-potential-biomarker-involved-in-the-modulation-of-biological-characteristics-of-residual-colorectal-cancer-cells-following-chemoradiotherapy
#13
Zhengting Chen, Xinyi Cai, Li Chang, Yaoxiong Xia, Li Wang, Yu Hou, Lan Li, Dingguo Pan, Furong Li, Shan Liu, Wei Xiong, Wenhui Li
Concurrent radiotherapy and chemotherapy is a widely used, comprehensive treatment for rectal cancer. By studying the impact of concurrent chemoradiotherapy on the invasion and migration of colorectal cancer (CRC) cells and researching the associated molecular mechanisms, the present study aimed to provide a novel method to improve the therapeutic effect of this treatment against CRC. Human HCT116 and HT29 CRC cells were simultaneously treated with 4 Gy of 6 MV X-rays and 10 µmol/l 5-fluorouracil to establish a residual cell model...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29532890/long-non-coding-rna-tcf7-predicts-the-progression-and-facilitates-the-growth-and-metastasis-of-colorectal-cancer
#14
Fu-Shu Jin, Hong-Mei Wang, Xiao-Yan Song
Long non-coding RNA (lnc)TCF7 has been reported to promote the self‑renewal of human cancer stem cells, and enhance the aggressiveness of human non‑small cell lung cancer and hepatocellular carcinoma cells. However, the effect of lncTCF7 on colorectal cancer (CRC) tumorigenesis and progression is currently unclear. In the present study, reverse transcription‑quantitative polymerase chain reaction results demonstrated that lncTCF7 expression was higher in CRC tissues compared with adjacent normal tissues and was significantly associated with tumor size, differentiation degree, tumor‑node‑metastasis grade, lymph node metastasis and invasion depth...
March 9, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29531162/-lnc-chop-promotes-immunosuppressive-function-of-myeloid-derived-suppressor-cells-in-tumor-and-inflammatory-environments
#15
Yunhuan Gao, Tiantian Wang, Yuanyuan Li, Yuan Zhang, Rongcun Yang
Myeloid-derived suppressor cells (MDSCs) are major regulators of immune responses in cancer. Both C/EBP homologous protein (CHOP) and C/EBPβ play a critical role in regulating immunosuppressive function of MDSCs. In this study, we identified a novel long noncoding RNA termed as lnc-chop in MDSCs, which may interact with CHOP and the C/EBPβ isoform liver-enriched inhibitory protein. The binding of lnc-chop with both CHOP and the C/EBPβ isoform liver-enriched inhibitory protein promoted the activation of C/EBPβ and upregulated the expression of arginase-1, NO synthase 2, NADPH oxidase 2, and cyclooxygenase-2, which are related to the immunosuppressive function of MDSCs in inflammatory and tumor environments...
March 12, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/29519872/adipocyte-long-noncoding-rna-transcriptome-analysis-of-obese-mice-identified-lnc-leptin-which-regulates-leptin
#16
Kinyui Alice Lo, Shiqi Huang, Arcinas Camille Esther Walet, Zhi-Chun Zhang, Melvin Khee-Shing Leow, Meihui Liu, Lei Sun
Obesity induces profound transcriptome changes in adipocytes; recent evidence suggests that lncRNAs play key roles in this process. Here, we performed a comprehensive transcriptome study by RNA-Seq in adipocytes isolated from interscapular brown, inguinal and epididymal white adipose tissues in diet-induced obese mice. Our analysis reveals a set of obesity-dysregulated lncRNAs, many of which exhibit dynamic changes in fed vs. fasted state, potentially serving as novel molecular markers reflecting adipose energy status...
March 8, 2018: Diabetes
https://www.readbyqxmd.com/read/29512756/expression-profiles-of-long-noncoding-rnas-and-mrnas-in-post-cardiac-arrest-rat-brains
#17
Rong Liu, Xiaoxing Liao, Xin Li, Hongyan Wei, Qing Liang, Zuopeng Zhang, Meixian Yin, Xiaoyun Zeng, Zijing Liang, Chunlin Hu
To investigate long noncoding (lnc)-RNA and mRNA expression profiles in post‑cardiac arrest (CA) brains, an external transthoracic electrical current was applied for 8 min to induce CA (the CA group). A total of 4 rats received sham-operations and served as the blank control (BC) group. Upon return of spontaneous circulation (ROSC), lncRNA and mRNA expression in the rat cerebral cortex was assayed with high‑throughput Agilent lncRNA and mRNA microarrays. In total, 37 lncRNAs were upregulated and 21 lncRNAs were downregulated in the CA group, and 258 mRNA transcripts were differentially expressed with 177 mRNAs upregulated and 81 mRNAs downregulated in the CA group...
March 7, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29512696/identification-of-key-genes-and-long-non%C3%A2-coding-rnas-in-celecoxib%C3%A2-treated-lung-squamous-cell-carcinoma-cell-line-by-rna%C3%A2-sequencing
#18
Gang Li, Xuehai Wang, Qingsong Luo, Chongzhi Gan
Celecoxib is an inhibitor of cyclooxygenase-2, a gene that is often aberrantly expressed in the lung squamous cell carcinoma (LSQCC). The present study aims to provide novel insight into chemoprevention by celecoxib treatment. The human LSQCC cell line SK‑MES‑1 was treated with or without celecoxib and RNA‑sequencing (RNA‑seq) was performed on the Illumina HiSeq 2000 platform. Expression levels of genes or long non‑coding RNAs (lncRNAs) were calculated by Cufflinks software. Subsequently, differentially expressed genes (DEGs) and differentially expressed lncRNAs (DE‑LNRs) between the two groups were selected using the limma package and LNCipedia 3...
March 1, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29509242/correlation-between-long-strand-non-coding-rna-gass-expression-and-prognosis-of-cervical-cancer-patients
#19
Y Li, Y-P Wan, Y Bai
OBJECTIVE: Cervical cancer is the second popular female specific malignant tumors. Long-strand non-coding RNA (lncRNA) GAS5 (Growth Arrest Specific 5) participates in pathological processes of various malignant tumors. This study aimed at investigating the correlation between lncRNA GAS5 expression and prognosis of cervical cancer patients. PATIENTS AND METHODS: Cancer tissues were collected from 48 cervical cancer patients. GAS5 expression in cervical cancer cells was determined by qRT-PCR and in situ hybridization (ISH)...
February 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29509239/prognostic-value-of-long-non-coding-rna-host2-expression-and-its-tumor-promotive-function-in-human-osteosarcoma
#20
X-D Qi, S-Y Xu, Y Song
OBJECTIVE: Aberrant expression of long noncoding RNA (lncRNA) is associated with carcinogenesis of various tumors. The aim of current study was to explore the clinical significance and biological function of long non-coding RNA HOST2 (lnc-HOST2) in patients with osteosarcoma. PATIENTS AND METHODS: The expression of lnc-HOST2 was detected by quantitative Real-time PCR (qRT-PCR) in 163 osteosarcoma specimens and matched the normal tissues. The significance of lnc-HOST2 as a prognostic factor as well as its relationship with survival was determined...
February 2018: European Review for Medical and Pharmacological Sciences
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