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Osteoarthritis biomarkers

Alyssa K Carlson, Rachel A Rawle, Erik Adams, Mark C Greenwood, Brian Bothner, Ronald K June
Osteoarthritis affects over 250 million individuals worldwide. Currently, there are no options for early diagnosis of osteoarthritis, demonstrating the need for biomarker discovery. To find biomarkers of osteoarthritis in human synovial fluid, we used high performance liquid-chromatography mass spectrometry for global metabolomic profiling. Metabolites were extracted from human osteoarthritic (n = 5), rheumatoid arthritic (n = 3), and healthy (n = 5) synovial fluid, and a total of 1233 metabolites were detected...
March 15, 2018: Biochemical and Biophysical Research Communications
Gulsah Gundogdu, Koksal Gundogdu
Adropin is newly discovered peptide hormone. Osteoarthritis (OA) is a kind of joint disease characterized by progressive joint cartilage loss and joint pain. The present study was carried out to investigate adropin and tumor necrosis factor alpha (TNF-α) levels and the relationship between adropin in patients with knee OA classified by Kellgren-Lawrence (KL). A total of 60 knee OA patients and 30 healthy controls were included in this study. KL grading was carried out using the radiographic findings. Demographic characteristics and laboratory parameters were recorded...
March 16, 2018: Clinical Rheumatology
Christelle Sanchez, Gabriel Mazzucchelli, Cécile Lambert, Fanny Comblain, Edwin DePauw, Yves Henrotin
OBJECTIVE: Osteoarthritis (OA) is characterized by cartilage degradation but also by other joint tissues modifications like subchondral bone sclerosis. In this study, we used a proteomic approach to compare secretome of osteoblast isolated from sclerotic (SC) or non sclerotic (NSC) area of OA subchondral bone. DESIGN: Secretome was analyzed using differential quantitative and relative label free analysis on nanoUPLC G2 HDMS system. mRNA of the more differentially secreted proteins were quantified by RT-PCR in cell culture from 5 other patients...
2018: PloS One
Felipe Rivas, Osama K Zahid, Heidi L Reesink, Bridgette T Peal, Alan J Nixon, Paul L DeAngelis, Aleksander Skardal, Elaheh Rahbar, Adam R Hall
Hyaluronan (or hyaluronic acid, HA) is a ubiquitous molecule that plays critical roles in numerous physiological functions in vivo, including tissue hydration, inflammation, and joint lubrication. Both the abundance and size distribution of HA in biological fluids are recognized as robust indicators of various pathologies and disease progressions. However, such analyses remain challenging because conventional methods are not sufficiently sensitive, have limited dynamic range, and/or are only semi-quantitative...
March 12, 2018: Nature Communications
Karen L Posey, Francoise Coustry, Jacqueline T Hecht
Cartilage oligomeric matrix protein (COMP) is a large pentameric glycoprotein that interacts with multiple extracellular matrix proteins in cartilage and other tissues. While, COMP is known to play a role in collagen secretion and fibrillogenesis, chondrocyte proliferation and mechanical strength of tendons, the complete range of COMP functions remains to be defined. COMPopathies describe pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (MED), two skeletal dysplasias caused by autosomal dominant COMP mutations...
March 9, 2018: Matrix Biology: Journal of the International Society for Matrix Biology
Alexander Tack, Anirban Mukhopadhyay, Stefan Zachow
OBJECTIVE: To present a novel method for automated segmentation of knee menisci from MRIs. To evaluate quantitative meniscal biomarkers for osteoarthritis (OA) estimated thereof. METHOD: A segmentation method employing convolutional neural networks in combination with statistical shape models was developed. Accuracy was evaluated on 88 manual segmentations. Meniscal volume, tibial coverage, and meniscal extrusion were computed and tested for differences between groups of OA, joint space narrowing (JSN), and WOMAC pain...
March 8, 2018: Osteoarthritis and Cartilage
Xiaobin Huang, Janine N Post, Leilei Zhong, Jeroen Leijten, Staffan Larsson, Marcel Karperien, André Struglics
OBJECTIVE: To explore the involvement of the WNT and BMP antagonists Dickkopf-related protein 1 (DKK1), Frizzled-related protein (FRZB) and Gremlin 1 (GREM1) in knee injury and osteoarthritis (OA). DESIGN: The antagonists were immunoassayed in synovial fluid from a cross-sectional cohort of 9 knee healthy reference subjects, patients with recent (0-77 days, n=158) or old (1-37 years, n=50) knee injuries, and OA (n=22). Cartilage (ARGS-aggrecan, cartilage oligomeric matrix protein and C2C type II collagen) and other biomarkers were assessed in synovial fluid in a subset of samples...
March 8, 2018: Osteoarthritis and Cartilage
Pierre Maudens, Christian Alexander Seemayer, François Pfefferlé, Olivier Jordan, Eric Allémann
This study aimed to formulate nanocrystal-polymer particles containing the potent p38α/β MAPK inhibitor PH-797804 (PH-NPPs) and to test their extended-release properties over months in comparison to those of conventional PH microparticles for the intra-articular treatment of inflammatory and mechanistic murine models mirroring aspects of human osteoarthritis (OA). The steps of the study were (i) to formulate PH nanocrystals (wet milling), (ii) to encapsulate a high payload of PH nanocrystals in fluorescent particles (spray drying), (iii) to assess in vitro drug release, (iv) to evaluate PH-NPP toxicity to human OA synoviocytes (MTT test), (v) to investigate the in vivo bioactivity of the particles in mice in an inflammatory antigen-induced arthritis (AIA) model (using histology and RT-qPCR) and (vi) to investigate the in vivo bioactivity of the particles in the OA model obtained by mechanistic surgical destabilization of the medial meniscus (DMM) (using histology, micro-CT, and multiplex ELISA)...
March 7, 2018: Journal of Controlled Release: Official Journal of the Controlled Release Society
Erik Lindström, Biljana Rizoska, Karin Tunblad, Charlotte Edenius, Alison M Bendele, Don Maul, Michael Larson, Neha Shah, Valerie Yoder Otto, Chris Jerome, Urszula Grabowska
BACKGROUND: MIV-711 is a highly potent and selective cathepsin K inhibitor. The current article summarizes the therapeutic effects of MIV-711 on joint pathology in rabbits subjected to anterior cruciate ligament transection (ACLT), and the prophylactic effects on joint pathology in dogs subjected to partial medial meniscectomy, two surgical models of osteoarthritis (OA). METHODS: Starting 1 week after surgery, rabbits were dosed daily via oral gavage with either MIV-711 or vehicle (n = 7/group) for 7 weeks...
March 9, 2018: Journal of Translational Medicine
Ke Lu, Tianshu Shi, Lan Li, Kaijia Zhang, Xiaobo Zhu, Siyu Shen, Fei Yu, Huajian Teng, Xiang Gao, Huangxian Ju, Wei Wang, Qing Jiang
Zhuangguguanjie formulation (ZG) can provide noticeable relief from joint pain in patients suffering from knee osteoarthritis (OA). However, the underlying mechanism has not been fully described. Male C57BL/6 mice were administered either ZG or normal saline (NS) following surgical destabilization of the medial meniscus (DMM). At weeks 4, 6 and 8 (post-surgery), knee joints were harvested and assessed with Safranin-O staining. Blood serum was collected and tested. In vitro analysis was carried out to evaluate the effects of ZG on the expression of the OA-related genes...
2018: American Journal of Translational Research
M J McAllister, M Chemaly, A J Eakin, D S Gibson, V E McGilligan
Osteoarthritis was previously thought of as 'wear and tear' as humans age, however there is increasing evidence to support an inflammatory theory. The NLRP3 inflammasome has been implicated in the pathogenesis of a number of arthritic disorders, producing proinflammatory cytokines and degradative enzymes such as IL-1β, TNF-α and MMP-3 which drive cartilage degeneration and synovial inflammation. This review aims to summarise the evidence of NLRP3 involvement in osteoarthritis. Currently, treatment options focus on management of the disease and to date there is no cure...
February 27, 2018: Osteoarthritis and Cartilage
Varvara Trachana, Eleni Ntoumou, Lydia Anastasopoulou, Aspasia Tsezou
MicroRNAs are small non-coding RNA species with the ability to post-transcriptionally control the expression of multiple genes, that have gained substantial interest because of their expression alterations that accompanies aging and possible age-related pathologies. Given the constant rise in the number of patients suffering from age-related diseases -due to the increase of the aging population in the western world- the exploration of the role of specific microRNAs in the etiopathology of these diseases is expected to have great impact...
February 26, 2018: Mechanisms of Ageing and Development
Zi Wang, Jialei Hu, Yue Pan, Yujia Shan, Liqun Jiang, Xia Qi, Li Jia
Osteoarthritis (OA), the most prevalent chronic and degenerative joint disease, is characterized by articular cartilage degradation and chondrocyte injury. Increased cell apoptosis and defective cell autophagy in chondrocytes are a feature of degenerative cartilage. MicroRNAs (miRNAs) have been identified as potential regulators of OA. This study aimed to determine the potential role of miR-140-5p and miR-149 in apoptosis, autophagy, and proliferation in human primary chondrocytes and investigate the underlying mechanism...
February 27, 2018: Inflammation
Myung-Ku Kim, Chul-Won Ha, Yong In, Sung-Do Cho, Eui-Sung Choi, Jeong-Ku Ha, Ju-Hong Lee, Jae-Doo Yoo, Seong-Il Bin, Choong-Hyeok Choi, Hee-Soo Kyung, Myung-Chul Lee
OBJECTIVE: To test the clinical efficacy of TissueGene-C (TG-C), a cell and gene therapeutic for osteoarthritis consisting of non-transformed and transduced chondrocytes (3:1), retrovirally transduced to overexpress TGF-β1. DESIGN: We randomly assigned 163 with knee osteoarthritis to receive intra-articular TG-C or placebo in Kellgren-Lawrence grade 3 patients for clinical trial. Primary efficacy measures included criteria for subjective assessment by International Knee Documentation Committee (IKDC) and pain severity by Visual Analog Scale (VAS) for 52 weeks...
February 27, 2018: Human Gene Therapy. Clinical Development
Dharmendra Choudhary, Priyanka Kothari, Ashish Kumar Tripathi, Sonu Singh, Sulekha Adhikary, Naseer Ahmad, Sudhir Kumar, Kapil Dev, Vijay Kumar Mishra, Shubha Shukla, Rakesh Maurya, Prabhat R Mishra, Ritu Trivedi
BACKGROUND: Spinacia oleracea is an important dietary vegetable in India and throughout the world and has many beneficial effects. It is cultivated globally. However, its effect on osteoarthritis that mainly targets the cartilage cells remains unknown. In this study we aimed to evaluate the anti-osteoarthritic and chondro-protective effects of SOE on chemically induced osteoarthritis (OA). METHODS: OA was induced by intra-patellar injection of monosodium iodoacetate (MIA) at the knee joint in rats...
February 20, 2018: BMC Complementary and Alternative Medicine
Iffah Nadhira Madzuki, Seng Fong Lau, Nur Adeelah Che Ahmad Tantowi, Nur Iliyani Mohd Ishak, Suhaila Mohamed
The tropical herb Labisia pumila is traditionally used in facilitating childbirth and post-partum care. The effects of L. pumila leaf extract (LP) in explant cartilage culture and on postmenopausal osteoarthritis (OA) rat model were assessed. The LP (10, 25 and 50 µg/ml) or diclofenac (10 µg/ml) was added to the cartilage explants containing bovine IL-1β (20 ng/ml), to evaluate their direct effects on cartilage degradation. In the preclinical study, rats were grouped (n = 8) into: non-treated OA; OA + diclofenac (5 mg/kg); OA + LP extract (150 and 300 mg/kg); and healthy control...
February 19, 2018: Inflammopharmacology
Grace H Lo, Erika Schneider, Jeffrey B Driban, Lori Lyn Price, David J Hunter, Charles B Eaton, Marc C Hochberg, Rebecca D Jackson, C Kent Kwoh, Michael C Nevitt, John A Lynch, Timothy E McAlindon
OBJECTIVE: Osteoarthritis (OA) is a disease with a substantial public health burden. Quantitative assessments of periarticular bone may be a biomarker capable of monitoring early disease progression. The purpose of this study was to evaluate whether measures of periarticular bone associate with longitudinal structural progression. METHODS: We conducted a 12-18 months longitudinal study using the Osteoarthritis Initiative (OAI). Participants received knee dual-energy x-ray absorptiometry (DXA), trabecular magnetic resonance (MR) imaging, and x-rays...
January 31, 2018: Seminars in Arthritis and Rheumatism
Arthur M Mandelin, Philip J Homan, Alexander M Shaffer, Carla M Cuda, Salina T Dominguez, Emily Bacalao, Mary Carns, Monique Hinchcliff, Jungwha Lee, Kathleen Aren, Anjali Thakrar, Anna B Montgomery, S Louis Bridges, Joan M Bathon, John P Atkinson, David A Fox, Eric L Matteson, Christopher D Buckley, Costantino Pitzalis, Deborah Parks, Laura B Hughes, Laura Geraldino-Pardilla, Robert Ike, Kristine Phillips, Kerry Wright, Andrew Filer, Stephen Kelly, Eric M Ruderman, Vince Morgan, Hiam Abdala-Valencia, Alexander V Misharin, G Scott Budinger, Elizabeth T Bartom, Richard M Pope, Harris Perlman, Deborah R Winter
OBJECTIVE: Currently, there are no reliable biomarkers for predicting therapeutic response in patients with rheumatoid arthritis (RA). The synovium may unlock critical information for determining efficacy as reduction in numbers of sublining synovial macrophages remains the most reproducible biomarker. Thus, a clinically actionable method for collection of synovial tissue, which can be analyzed using high-throughput strategies, must become a reality. METHODS: Rheumatologists at six United States academic sites were trained in minimally invasive ultrasound-guided synovial tissue biopsy...
February 13, 2018: Arthritis & Rheumatology
Miguel Tofiño-Vian, Maria Isabel Guillén, Maria José Alcaraz
Extracellular vesicles (EVs) are attracting increasing interest since they might represent a more convenient therapeutic tool with respect to their cells of origin. In the last years much time and effort have been expended to determine the biological properties of EVs from mesenchymal stem cells (MSCs) and other sources. The immunoregulatory, anti-inflammatory and regenerative properties of MSC EVs have been demonstrated in in vitro studies and animal models of rheumatoid arthritis or osteoarthritis. This cell-free approach has been proposed as a possible better alternative to MSC therapy in autoimmune conditions and tissue regeneration...
February 7, 2018: Biochemical Pharmacology
J M Farber
No abstract text is available yet for this article.
February 7, 2018: Osteoarthritis and Cartilage
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