Yang Bi, Zhiping Deng, Weimin Ni, Ruben Shrestha, Dasha Savage, Thomas Hartwig, Sunita Patil, Su Hyun Hong, Zhenzhen Zhang, Juan A Oses-Prieto, Kathy H Li, Peter H Quail, Alma L Burlingame, Shou-Ling Xu, Zhi-Yong Wang
O-GlcNAc modification plays important roles in metabolic regulation of cellular status. Two homologs of O-GlcNAc transferase, SECRET AGENT (SEC) and SPINDLY (SPY), which have O-GlcNAc and O-fucosyl transferase activities, respectively, are essential in Arabidopsis but have largely unknown cellular targets. Here we show that AtACINUS is O-GlcNAcylated and O-fucosylated and mediates regulation of transcription, alternative splicing (AS), and developmental transitions. Knocking-out both AtACINUS and its distant paralog AtPININ causes severe growth defects including dwarfism, delayed seed germination and flowering, and abscisic acid (ABA) hypersensitivity...
February 11, 2021: Nature Communications