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Fucosyl transferase

Paul D Ling, Simon Y Long, Jian-Chao Zong, Sarah Y Heaggans, Xiang Qin, Gary S Hayward
Nearly 100 cases of lethal acute hemorrhagic disease in young Asian elephants have been reported worldwide. All tested cases contained high levels of elephant endotheliotropic herpesvirus (EEHV) DNA in pathological blood or tissue samples. Seven known major types of EEHVs have been partially characterized and shown to all belong to the novel Proboscivirus genus. However, the recently determined 206-kb EEHV4 genome proved to represent the prototype of a GC-rich branch virus that is very distinct from the previously published 180-kb EEHV1A, EEHV1B, and EEHV5A genomes, which all fall within an alternative AT-rich branch...
May 2016: MSphere
Song Gao, Liancheng Zhu, Huilin Feng, Zhenhua Hu, Shan Jin, Zuofei Song, Dawo Liu, Juanjuan Liu, Yingying Hao, Xiao Li, Bei Lin
The aim of this study was to identify differentially expressed genes (DEGs) in response to α1,2-fucosyl transferase (FUT1) gene transfection in epithelial ovarian cancer cells. Human whole-genome oligonucleotide microarrays were used to determine whether gene expression profile may differentiate the epithelial ovarian cell line Caov-3 transfected with FUT1 from the empty plasmid-transfected cells. Quantitative real-time PCR and immunohistochemical staining validated the microarray results. Gene expression profile identified 215 DEGs according to the selection criteria, in which 122 genes were upregulated and 93 genes were downregulated...
May 30, 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Si-Ming Zhang, Eric S Loker, John T Sullivan
The anterior pericardial wall of the snail Biomphalaria glabrata has been identified as a site of hemocyte production, hence has been named the amebocyte-producing organ (APO). A number of studies have shown that exogenous abiotic and biotic substances, including pathogen associated molecular patterns (PAMPs), are able to stimulate APO mitotic activity and/or enlarge its size, implying a role for the APO in innate immunity. The molecular mechanisms underlying such responses have not yet been explored, in part due to the difficulty in obtaining sufficient APO tissue for gene expression studies...
March 2016: Developmental and Comparative Immunology
A Merlotti, E Dantas, F Remes Lenicov, A Ceballos, C Jancic, A Varese, J Rubione, S Stover, J Geffner, J Sabatté
STUDY QUESTION: Could seminal plasma clusterin play a role in the uptake of stress-damaged proteins by dendritic cells? SUMMARY ANSWER: Seminal plasma clusterin, but not serum clusterin, promotes the uptake of stress-damaged proteins by dendritic cells via DC-SIGN. WHAT IS KNOWN ALREADY: Clusterin is one of the major extracellular chaperones. It interacts with a variety of stressed proteins to prevent their aggregation, guiding them for receptor-mediated endocytosis and intracellular degradation...
July 2015: Human Reproduction
Nenad Tomasevic, Kenneth Luehrsen, Mark Baer, Varghese Palath, David Martinez, Jason Williams, Christina Yi, Swathi Sujatha-Bhaskar, Rohini Lanke, John Leung, Wendy Ching, Andreia Lee, Lu Bai, Geoffrey Yarranton, Christopher Bebbington
EphA3 is expressed in solid tumors and leukemias and is an attractive target for the therapy. We have generated a panel of Humaneered® antibodies to the ligand-binding domain using a Fab epitope-focused library that has the same specificity as monoclonal antibody mIIIA4. A high-affinity antibody was selected that competes with the mIIIA4 antibody for binding to EphA3 and has an improved affinity of ∼1 nM. In order to generate an antibody with potent cell-killing activity the variable regions were assembled with human IgG1k constant regions and expressed in a Chinese hamster ovary (CHO) cell line deficient in fucosyl transferase...
December 2014: Growth Factors
David Bonar, Franz-Georg Hanisch
The disaccharide N,N'-di-N-acetyllactose diamine (LacdiNAc, GalNAcβ1-4GlcNAcβ) is found in a limited number of extracellular matrix glycoproteins and neuropeptide hormones indicating a protein-specific transfer of GalNAc by the glycosyltransferases β4GalNAc-T3/T4. Whereas previous studies have revealed evidence for peptide determinants as controlling elements in LacdiNAc biosynthesis, we report here on an entirely independent conformational control of GalNAc transfer by single TFF (Trefoil factor) domains as high stringency determinants...
October 24, 2014: Journal of Biological Chemistry
Minyu Wang, Jilin Wang, Xuan Kong, Huimin Chen, Yingchao Wang, Miao Qin, Yanwei Lin, Haoyan Chen, Jie Xu, Jie Hong, Ying-Xuan Chen, Weiping Zou, Jing-Yuan Fang
In this study we investigated the biological role and mechanism of miR-198 in colorectal carcinoma (CRC). MiR-198 expression was shown to exhibit a strongly negative correlation with lymph node invasion, distant metastasis and patient survival in examinations of colorectal cancer tissues and paired normal colorectal mucosa tissues. fucosyl transferase 8 (FUT8) was identified as a potential target of miR-198 in bioinformatics analysis and luciferase reporter assays. Overexpression of miR-198 in CRC cell lines decreased FUT8 levels as shown by immunofluorescence analysis, and inhibited cell proliferation, migration, and invasion...
2014: Scientific Reports
Max Efui Annani-Akollor, Shujing Wang, Jianhui Fan, Linhua Liu, Arshad Ahmed Padhiar, Jianing Zhang
The Notch signalling pathway is essential for proper cell growth and development. Many factors interact in the cellular context to bring about its effects. Critical to this process is the influence of Pofut1 whose function is to fucosylate Notch receptors and ligands on the cell surface for proper interaction. Of the three liver cell lines, HepG2, SMMC-7721 (hepatoma cell lines) and L-02 (normal fetal liver cell line) were investigated. In the current study, Pofut1 was silenced in L-02 due to its significantly high level of expression, (P<0...
July 2014: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Bertrand Vabres, Stéphanie Le Bas-Bernardet, David Riochet, Yan Chérel, David Minault, Jérémy Hervouet, Yvette Ducournau, Anne Moreau, Véronique Daguin, Flora Coulon, Annaïck Pallier, Sophie Brouard, Simon C Robson, Mark B Nottle, Peter J Cowan, Eric Venturi, Pascal Mermillod, Philippe Brachet, Cesare Galli, Irina Lagutina, Roberto Duchi, Jean-Marie Bach, Gilles Blancho, Jean-Paul Soulillou, Bernard Vanhove
BACKGROUND: Human corneal allografting is an established procedure to cure corneal blindness. However, a shortage of human donor corneas as well as compounding economic, cultural, and organizational reasons in many countries limit its widespread use. Artificial corneas as well as porcine corneal xenografts have been considered as possible alternatives. To date, all preclinical studies using de-cellularized pig corneas have shown encouraging graft survival results; however, relatively few studies have been conducted in pig to non-human primate (NHP) models, and particularly using genetically engineered donors...
September 2014: Xenotransplantation
Jana Krenkova, Akos Szekrenyes, Zsolt Keresztessy, Frantisek Foret, Andras Guttman
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate moieties from glycoproteins. The glutathione-S-transferase-fusion PNGase F (PNGase F-GST) was expressed in Escherichia coli using regular vector technology. The monolithic pore surface was functionalized with glutathione via a succinimidyl-6-(iodoacetyl-amino)-hexanoate linker and the specific affinity of GST toward glutathione was utilized for the oriented coupling...
December 27, 2013: Journal of Chromatography. A
Manjeri A Venkatachalam, Joel M Weinberg
Shen et al. exploit glycobiology to dampen transforming growth factor-β (TGF-β) signaling and ameliorate renal fibrosis after ureteral obstruction. Core fucosylation of N-linked oligosaccharides in TGF-β receptors is required for receptor function. Adenoviruses expressing Fut8-fucosyl transferase-shRNA inhibited receptor fucosylation, decreased tubule TGF-β signaling, and reduced fibrosis. Fut8-shRNA interferes with core fucosylation of other receptors also. Regardless, this first attempt to capitalize on a new aspect of TGF-β receptor function provides a basis for further research...
July 2013: Kidney International
Young-Wook Chin, Jin-Byung Park, Yong-Cheol Park, Kyoung Heon Kim, Jin-Ho Seo
Wild-type Corynebacterium glutamicum was metabolically engineered to convert glucose and mannose into guanosine 5'-diphosphate (GDP)-L-fucose, a precursor of fucosyl-oligosaccharides, which are involved in various biological and pathological functions. This was done by introducing the gmd and wcaG genes of Escherichia coli encoding GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, respectively, which are known as key enzymes in the production of GDP-L-fucose from GDP-D-mannose...
June 2013: Bioprocess and Biosystems Engineering
Vinay Singh Tanwar, Mandeep P Chand, Jitender Kumar, Gaurav Garg, Sandeep Seth, Ganesan Karthikeyan, Shantanu Sengupta
Vitamin B(12) is an essential micronutrient synthesized by microorganisms. Mammals including humans have evolved ways for transport and absorption of this vitamin. Deficiency of vitamin B(12) (either due to low intake or polymorphism in genes involved in absorption and intracellular transport of this vitamin) has been associated with various complex diseases. Genome-wide association studies have recently identified several common single nucleotide polymorphisms (SNPs) in fucosyl transferase 2 gene (FUT2) to be associated with levels of vitamin B(12)-the strongest association was with a non-synonymous SNP rs602662 in this gene...
February 15, 2013: Gene
Tomoko Yamakawa, Tomonori Ayukawa, Kenji Matsuno
Notch is a single-pass transmembrane receptor that mediates the local cell-cell interactions necessary for many cell-fate decisions. The extra cellular domain of Notch contains a tandem array of epidermal growth factor-like (EGF-like) repeats. Some of these EGF-like repeats are O-fucosylated by protein O-fucosyltransferase 1 (O-fut1), which is essential for Notch signaling in Drosophila and mouse. This O-fucose is further modified by Fringe, a GlcNAc transferase and other glycosyltransferases (O-fut1 in Drosophila and Pofut1 in mouse), to form an O-linked tetrasaccharide, which modulates Notch's selective binding to its ligands...
2012: Advances in Experimental Medicine and Biology
Maho Amano, Ryo Hashimoto, Shin-Ichiro Nishimura
Gene knock-out of C-type lectin receptors expressed in dendritic cells induced significant alteration of serum N-glycans compared with that of gender-matched controls. Glycotyping analysis suggested that putative-core fucosylation is strongly influenced by differences in the dominant mechanisms after carbohydrate recognition by pattern-recognition receptors, endocytosis of ligands, or induction of cytokines/chemokines. However, the loss of galectin-9, a ligand for T-helper type 1-specific cell-surface molecule, did not affect most N-glycan profiles...
February 13, 2012: Chembiochem: a European Journal of Chemical Biology
Mariana Barboza, Janneth Pinzon, Saumya Wickramasinghe, John W Froehlich, Isabelle Moeller, Jennifer T Smilowitz, L Renee Ruhaak, Jincui Huang, Bo Lönnerdal, J Bruce German, Juan F Medrano, Bart C Weimer, Carlito B Lebrilla
Human milk lactoferrin (hmLF) is the most abundant glycoprotein present in human milk and displays a broad range of protective functions in the gut of newborn infants. hmLF is N-glycosylated, but little is known about the lactation stage-related development of the glycosylation phenotype. hmLF glycosylation from milk samples from five donors during the first 10 weeks of lactation was assessed and observed to be more diverse than previously reported. During this period dynamic changes in glycosylation were observed corresponding to a decrease in glycosylation in the second week followed by an increase in total glycosylation as well as higher order fucosylation thereafter...
June 2012: Molecular & Cellular Proteomics: MCP
S Le Bas-Bernardet, X Tillou, N Poirier, N Dilek, M Chatelais, J Devallière, B Charreau, D Minault, J Hervouet, K Renaudin, C Crossan, L Scobie, P J Cowan, A J F d'Apice, C Galli, E Cozzi, J P Soulillou, B Vanhove, G Blancho
Galactosyl-transferase knockout (GT-KO) pigs represent the latest major progress to reduce immune reactions in xenotransplantation. However, their organs are still subject to rapid humoral rejection involving complement activation requiring the ongoing development of further genetic modifications in the pig. In a pig-to-baboon renal transplantation setting, we have used donor pigs that are not only GT-KO, but also transgenic for human CD55 (hCD55), hCD59, hCD39, and fucosyl-transferase (hHT). We studied kidney xenograft survival, physiological and immunologic parameters, xenogeneic rejection characteristics, as well as viral transmission aspects among two groups of baboons: control animals (n = 2), versus those (n = 4) treated with a cocktail of cyclophosphamide, tacrolimus, mycophenolate mofetil, steroids, and a recombinant human C1 inhibitor...
November 2011: Transplantation Proceedings
Sonia Serna, Shi Yan, Manuel Martin-Lomas, Iain B H Wilson, Niels-Christian Reichardt
Two recombinant fucosyltransferases were employed as synthetic tools in the chemoenzymatic synthesis of core fucosylated N-glycan structures. Enzyme substrates were rapidly identified by incubating a microarray of synthetic N-glycans with the transferases and detecting the presence of core fucose with four lectins and one antibody. Selected substrates were then enzymatically fucosylated in solution on a preparative scale and characterized by NMR and MS. With this approach the chemoenzymatic synthesis of a series of α1,3-, α1,6-, and difucosylated structures was accomplished in very short time and with high yields, which otherwise would have required extensive additional synthetic effort and a complete redesign of existing synthetic routes...
October 19, 2011: Journal of the American Chemical Society
K Kubushiro, K Tsukazaki, Y Sakuma, Y Akiba, M Sakayori, R Aoki, S Yazawa, S Nozawa
Cells of uterine endometrial cancer cell line SNG-II were classified into two groups according to their reactivity with anti-uterine endometrial cancer monoclonal antibody (MSN-1), whose recognition antigen is mainly the Lewis(b) antigens; those that strongly reacted with MSN-1 (SNG-S group) and those that weakly reacted with it (SNG-W group). The SNG-S showed a higher activity of a 1-->4-fucosyltransferase activity than that of the SNG-W. The expression of Lewis(b) antigen was stronger in the SNG-S than that in the SNG-W...
January 1995: International Journal of Oncology
Moira E Galway, Ryan C Eng, John W Schiefelbein, Geoffrey O Wasteneys
The glycosyl transferase encoded by the cellulose synthase-like gene CSLD3/KJK/RHD7 (At3g03050) is required for cell wall integrity during root hair formation in Arabidopsis thaliana but it remains unclear whether it contributes to the synthesis of cellulose or hemicellulose. We identified two new alleles, root hair-defective (rhd) 7-1 and rhd7-4, which affect the C-terminal end of the encoded protein. Like root hairs in the previously characterized kjk-2 putative null mutant, rhd7-1 and rhd7-4 hairs rupture before tip growth but, depending on the growth medium and temperature, hairs are able to survive rupture and initiate tip growth, indicating that these alleles retain some function...
May 2011: Planta
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