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Fucosyl transferase

Devin K Smith, Danielle M Jones, Jonathan B R Lau, Edward R Cruz, Elizabeth Brown, Jeffrey F Harper, Ian S Wallace
During pollen-pistil interactions in angiosperms, the male gametophyte (pollen) germinates to produce a pollen tube. To fertilize ovules located within the female pistil, the pollen tube must physically penetrate specialized tissues. Whereas the process of pollen tube penetration through the pistil has been anatomically well described, the genetic regulation remains poorly understood. In this study, we identify a novel Arabidopsis ( Arabidopsis thaliana ) gene, O-FUCOSYLTRANSFERASE1 ( AtOFT1 ), which plays a key role in pollen tube penetration through the stigma-style interface...
April 2018: Plant Physiology
Tzu-Hao Tseng, Tzu-Wen Lin, Chien-Yu Chen, Chein-Hung Chen, Jung-Lee Lin, Tsui-Ling Hsu, Chi-Huey Wong
The core fucosylation of N-glycans on glycoproteins is catalyzed by fucosyltransferase 8 (FUT8) in mammalian cells and is involved in various biological functions, such as protein function, cancer progression, and postnatal development. The substrate specificity of FUT8 toward bi-antennary N-glycans has been reported, but it is unclear with regard to tri-antennary and tetra-antennary glycans. Here, we examined the specificity and activity of human FUT8 toward tri- and tetra-antennary N-glycans in the forms of glycopeptides...
July 11, 2017: Journal of the American Chemical Society
Breeanna R Urbanowicz, Vivek S Bharadwaj, Markus Alahuhta, Maria J Peña, Vladimir V Lunin, Yannick J Bomble, Shuo Wang, Jeong-Yeh Yang, Sami T Tuomivaara, Michael E Himmel, Kelley W Moremen, William S York, Michael F Crowley
The mechanistic underpinnings of the complex process of plant polysaccharide biosynthesis are poorly understood, largely because of the resistance of glycosyltransferase (GT) enzymes to structural characterization. In Arabidopsis thaliana, a glycosyl transferase family 37 (GT37) fucosyltransferase 1 (AtFUT1) catalyzes the regiospecific transfer of terminal 1,2-fucosyl residues to xyloglucan side chains - a key step in the biosynthesis of fucosylated sidechains of galactoxyloglucan. We unravel the mechanistic basis for fucosylation by AtFUT1 with a multipronged approach involving protein expression, X-ray crystallography, mutagenesis experiments and molecular simulations...
September 2017: Plant Journal: for Cell and Molecular Biology
Vignesh Venkatakrishnan, Macarena P Quintana-Hayashi, Maxime Mahu, Freddy Haesebrouck, Frank Pasmans, Sara K Lindén
Brachyspira hyodysenteriae causes swine dysentery (SD), leading to global financial losses to the pig industry. Infection with this pathogen results in an increase in B. hyodysenteriae binding sites on mucins, along with increased colonic mucin secretion. We predict that B. hyodysenteriae modifies the glycosylation pattern of the porcine intestinal mucus layer to optimize its host niche. We characterized the swine colonic mucin O-glycome and identified the differences in glycosylation between B. hyodysenteriae-infected and noninfected pigs...
April 7, 2017: Journal of Proteome Research
Nivedita Kuila, Kasturi Bala Nayak, Arundhati Halder, Subramaniam Agatheeswaran, Ghanashyam Biswas, Sutapa Biswas, Naresh Chandra Pattnayak, Soumen Chakraborty
Although BCR-ABL is the hallmark genetic abnormality of chronic myeloid leukemia (CML), secondary molecular events responsible for the evolution of the disease to blast crisis are yet to be deciphered. Taking into account the significant association of ecotropic viral integration site I (EVI1) in CML drug resistance, it is necessary to decipher the other roles played by EVI1 in CML disease progression. In this regard, we cross-hybridized three microarray datasets and deduced a set of 11 genes that seems to be regulated by EVI1 in CML...
August 2017: Leukemia & Lymphoma
Tiehai Li, Min Huang, Lin Liu, Shuo Wang, Kelley W Moremen, Geert-Jan Boons
A divergent chemoenzymaytic approach for the preparation of core-fucosylated and core-unmodified asymmetrical N-glycans from a common advances precursor is described. An undecasaccharide was synthesized by sequential chemical glycosylations of an orthogonally protected core fucosylated hexasaccharide that is common to all mammalian core fucosylated N-glycans. Antennae-selective enzymatic extension of the undecasaccharide using a panel of glycosyl transferases afforded core fucosylated asymmetrical triantennary N-glycan isomers, which are potential biomarkers for breast cancer...
December 23, 2016: Chemistry: a European Journal
Paul D Ling, Simon Y Long, Jian-Chao Zong, Sarah Y Heaggans, Xiang Qin, Gary S Hayward
Nearly 100 cases of lethal acute hemorrhagic disease in young Asian elephants have been reported worldwide. All tested cases contained high levels of elephant endotheliotropic herpesvirus (EEHV) DNA in pathological blood or tissue samples. Seven known major types of EEHVs have been partially characterized and shown to all belong to the novel Proboscivirus genus. However, the recently determined 206-kb EEHV4 genome proved to represent the prototype of a GC-rich branch virus that is very distinct from the previously published 180-kb EEHV1A, EEHV1B, and EEHV5A genomes, which all fall within an alternative AT-rich branch...
May 2016: MSphere
Song Gao, Liancheng Zhu, Huilin Feng, Zhenhua Hu, Shan Jin, Zuofei Song, Dawo Liu, Juanjuan Liu, Yingying Hao, Xiao Li, Bei Lin
The aim of this study was to identify differentially expressed genes (DEGs) in response to α1,2-fucosyl transferase (FUT1) gene transfection in epithelial ovarian cancer cells. Human whole-genome oligonucleotide microarrays were used to determine whether gene expression profile may differentiate the epithelial ovarian cell line Caov-3 transfected with FUT1 from the empty plasmid-transfected cells. Quantitative real-time PCR and immunohistochemical staining validated the microarray results. Gene expression profile identified 215 DEGs according to the selection criteria, in which 122 genes were upregulated and 93 genes were downregulated...
September 2016: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
Si-Ming Zhang, Eric S Loker, John T Sullivan
The anterior pericardial wall of the snail Biomphalaria glabrata has been identified as a site of hemocyte production, hence has been named the amebocyte-producing organ (APO). A number of studies have shown that exogenous abiotic and biotic substances, including pathogen associated molecular patterns (PAMPs), are able to stimulate APO mitotic activity and/or enlarge its size, implying a role for the APO in innate immunity. The molecular mechanisms underlying such responses have not yet been explored, in part due to the difficulty in obtaining sufficient APO tissue for gene expression studies...
March 2016: Developmental and Comparative Immunology
A Merlotti, E Dantas, F Remes Lenicov, A Ceballos, C Jancic, A Varese, J Rubione, S Stover, J Geffner, J Sabatté
STUDY QUESTION: Could seminal plasma clusterin play a role in the uptake of stress-damaged proteins by dendritic cells? SUMMARY ANSWER: Seminal plasma clusterin, but not serum clusterin, promotes the uptake of stress-damaged proteins by dendritic cells via DC-SIGN. WHAT IS KNOWN ALREADY: Clusterin is one of the major extracellular chaperones. It interacts with a variety of stressed proteins to prevent their aggregation, guiding them for receptor-mediated endocytosis and intracellular degradation...
July 2015: Human Reproduction
Nenad Tomasevic, Kenneth Luehrsen, Mark Baer, Varghese Palath, David Martinez, Jason Williams, Christina Yi, Swathi Sujatha-Bhaskar, Rohini Lanke, John Leung, Wendy Ching, Andreia Lee, Lu Bai, Geoffrey Yarranton, Christopher Bebbington
EphA3 is expressed in solid tumors and leukemias and is an attractive target for the therapy. We have generated a panel of Humaneered® antibodies to the ligand-binding domain using a Fab epitope-focused library that has the same specificity as monoclonal antibody mIIIA4. A high-affinity antibody was selected that competes with the mIIIA4 antibody for binding to EphA3 and has an improved affinity of ∼1 nM. In order to generate an antibody with potent cell-killing activity the variable regions were assembled with human IgG1k constant regions and expressed in a Chinese hamster ovary (CHO) cell line deficient in fucosyl transferase...
December 2014: Growth Factors
David Bonar, Franz-Georg Hanisch
The disaccharide N,N'-di-N-acetyllactose diamine (LacdiNAc, GalNAcβ1-4GlcNAcβ) is found in a limited number of extracellular matrix glycoproteins and neuropeptide hormones indicating a protein-specific transfer of GalNAc by the glycosyltransferases β4GalNAc-T3/T4. Whereas previous studies have revealed evidence for peptide determinants as controlling elements in LacdiNAc biosynthesis, we report here on an entirely independent conformational control of GalNAc transfer by single TFF (Trefoil factor) domains as high stringency determinants...
October 24, 2014: Journal of Biological Chemistry
Minyu Wang, Jilin Wang, Xuan Kong, Huimin Chen, Yingchao Wang, Miao Qin, Yanwei Lin, Haoyan Chen, Jie Xu, Jie Hong, Ying-Xuan Chen, Weiping Zou, Jing-Yuan Fang
In this study we investigated the biological role and mechanism of miR-198 in colorectal carcinoma (CRC). MiR-198 expression was shown to exhibit a strongly negative correlation with lymph node invasion, distant metastasis and patient survival in examinations of colorectal cancer tissues and paired normal colorectal mucosa tissues. fucosyl transferase 8 (FUT8) was identified as a potential target of miR-198 in bioinformatics analysis and luciferase reporter assays. Overexpression of miR-198 in CRC cell lines decreased FUT8 levels as shown by immunofluorescence analysis, and inhibited cell proliferation, migration, and invasion...
September 1, 2014: Scientific Reports
Max Efui Annani-Akollor, Shujing Wang, Jianhui Fan, Linhua Liu, Arshad Ahmed Padhiar, Jianing Zhang
The Notch signalling pathway is essential for proper cell growth and development. Many factors interact in the cellular context to bring about its effects. Critical to this process is the influence of Pofut1 whose function is to fucosylate Notch receptors and ligands on the cell surface for proper interaction. Of the three liver cell lines, HepG2, SMMC-7721 (hepatoma cell lines) and L-02 (normal fetal liver cell line) were investigated. In the current study, Pofut1 was silenced in L-02 due to its significantly high level of expression, (P<0...
July 2014: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Bertrand Vabres, Stéphanie Le Bas-Bernardet, David Riochet, Yan Chérel, David Minault, Jérémy Hervouet, Yvette Ducournau, Anne Moreau, Véronique Daguin, Flora Coulon, Annaïck Pallier, Sophie Brouard, Simon C Robson, Mark B Nottle, Peter J Cowan, Eric Venturi, Pascal Mermillod, Philippe Brachet, Cesare Galli, Irina Lagutina, Roberto Duchi, Jean-Marie Bach, Gilles Blancho, Jean-Paul Soulillou, Bernard Vanhove
BACKGROUND: Human corneal allografting is an established procedure to cure corneal blindness. However, a shortage of human donor corneas as well as compounding economic, cultural, and organizational reasons in many countries limit its widespread use. Artificial corneas as well as porcine corneal xenografts have been considered as possible alternatives. To date, all preclinical studies using de-cellularized pig corneas have shown encouraging graft survival results; however, relatively few studies have been conducted in pig to non-human primate (NHP) models, and particularly using genetically engineered donors...
September 2014: Xenotransplantation
Jana Krenkova, Akos Szekrenyes, Zsolt Keresztessy, Frantisek Foret, Andras Guttman
In this paper, we report on a novel oriented peptide-N-glycosidase F (PNGase F) immobilization approach onto methacrylate based monolithic support for rapid, reproducible and efficient release of the N-linked carbohydrate moieties from glycoproteins. The glutathione-S-transferase-fusion PNGase F (PNGase F-GST) was expressed in Escherichia coli using regular vector technology. The monolithic pore surface was functionalized with glutathione via a succinimidyl-6-(iodoacetyl-amino)-hexanoate linker and the specific affinity of GST toward glutathione was utilized for the oriented coupling...
December 27, 2013: Journal of Chromatography. A
Manjeri A Venkatachalam, Joel M Weinberg
Shen et al. exploit glycobiology to dampen transforming growth factor-β (TGF-β) signaling and ameliorate renal fibrosis after ureteral obstruction. Core fucosylation of N-linked oligosaccharides in TGF-β receptors is required for receptor function. Adenoviruses expressing Fut8-fucosyl transferase-shRNA inhibited receptor fucosylation, decreased tubule TGF-β signaling, and reduced fibrosis. Fut8-shRNA interferes with core fucosylation of other receptors also. Regardless, this first attempt to capitalize on a new aspect of TGF-β receptor function provides a basis for further research...
July 2013: Kidney International
Young-Wook Chin, Jin-Byung Park, Yong-Cheol Park, Kyoung Heon Kim, Jin-Ho Seo
Wild-type Corynebacterium glutamicum was metabolically engineered to convert glucose and mannose into guanosine 5'-diphosphate (GDP)-L-fucose, a precursor of fucosyl-oligosaccharides, which are involved in various biological and pathological functions. This was done by introducing the gmd and wcaG genes of Escherichia coli encoding GDP-D-mannose-4,6-dehydratase and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase, respectively, which are known as key enzymes in the production of GDP-L-fucose from GDP-D-mannose...
June 2013: Bioprocess and Biosystems Engineering
Vinay Singh Tanwar, Mandeep P Chand, Jitender Kumar, Gaurav Garg, Sandeep Seth, Ganesan Karthikeyan, Shantanu Sengupta
Vitamin B(12) is an essential micronutrient synthesized by microorganisms. Mammals including humans have evolved ways for transport and absorption of this vitamin. Deficiency of vitamin B(12) (either due to low intake or polymorphism in genes involved in absorption and intracellular transport of this vitamin) has been associated with various complex diseases. Genome-wide association studies have recently identified several common single nucleotide polymorphisms (SNPs) in fucosyl transferase 2 gene (FUT2) to be associated with levels of vitamin B(12)-the strongest association was with a non-synonymous SNP rs602662 in this gene...
February 15, 2013: Gene
Tomoko Yamakawa, Tomonori Ayukawa, Kenji Matsuno
Notch is a single-pass transmembrane receptor that mediates the local cell-cell interactions necessary for many cell-fate decisions. The extra cellular domain of Notch contains a tandem array of epidermal growth factor-like (EGF-like) repeats. Some of these EGF-like repeats are O-fucosylated by protein O-fucosyltransferase 1 (O-fut1), which is essential for Notch signaling in Drosophila and mouse. This O-fucose is further modified by Fringe, a GlcNAc transferase and other glycosyltransferases (O-fut1 in Drosophila and Pofut1 in mouse), to form an O-linked tetrasaccharide, which modulates Notch's selective binding to its ligands...
2012: Advances in Experimental Medicine and Biology
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