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https://www.readbyqxmd.com/read/28927239/tubulins-rhythms-and-cell-walls-in-poplar-leaves-it-s-all-in-the-timing
#1
Scott A Harding, Hao Hu, Batbayar Nyamdari, Liang-Jiao Xue, Radnaa Naran, Chung-Jui Tsai
Plant cell walls exhibit architectural and compositional changes throughout their development and in response to external cues. While tubulins are involved in cell wall biogenesis, much remains unknown about the scope of their involvement during the orchestration of this resource-demanding process. A transgenic approach coupled with cell wall compositional analysis, RNA-seq and mining of publicly available diurnal gene expression data was used to assess the involvement of tubulins in poplar leaf cell wall biogenesis...
September 12, 2017: Tree Physiology
https://www.readbyqxmd.com/read/28927194/finding-a-facile-way-for-the-bacterial-dna-transformation-by-biosynthesized-gold-nanoparticles
#2
Madhuree Kumari, Shipra Pandey, Aradhana Mishra, Chandra Shekhar Nautiyal
The major problem encountered during genetic manipulation of bacteria is the inability to get transformed because of their natural non-competency. In this study, to overcome this problem, a cost-effective method was developed by combining the properties of gold nanoparticles (GNPs) and the Yoshida effect. Various parameters, including GNP:plasmid ratio, pH and time, were optimized for stability of the GNP-plasmid conjugate. With non-competent Gram-negative cells, the efficiency ranged between 0.1 and 0.45 × 104 transformants μg-1, while the range was (0...
July 3, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28927007/carrier-multiplication-mechanisms-and-competing-processes-in-colloidal-semiconductor-nanostructures
#3
REVIEW
Stephen V Kershaw, Andrey L Rogach
Quantum confined semiconductor nanoparticles, such as colloidal quantum dots, nanorods and nanoplatelets have broad extended absorption spectra at energies above their bandgaps. This means that they can absorb light at high photon energies leading to the formation of hot excitons with finite excited state lifetimes. During their existence, the hot electron and hole that comprise the exciton may start to cool as they relax to the band edge by phonon mediated or Auger cooling processes or a combination of these...
September 18, 2017: Materials
https://www.readbyqxmd.com/read/28926765/texture-and-art-with-deep-neural-networks
#4
REVIEW
Leon A Gatys, Alexander S Ecker, Matthias Bethge
Although the study of biological vision and computer vision attempt to understand powerful visual information processing from different angles, they have a long history of informing each other. Recent advances in texture synthesis that were motivated by visual neuroscience have led to a substantial advance in image synthesis and manipulation in computer vision using convolutional neural networks (CNNs). Here, we review these recent advances and discuss how they can in turn inspire new research in visual perception and computational neuroscience...
September 16, 2017: Current Opinion in Neurobiology
https://www.readbyqxmd.com/read/28926752/response-bias-and-response-monitoring-evidence-from-healthy-older-adults-and-patients-with-mild-alzheimer-s-disease
#5
Rebecca G Deason, Michelle J Tat, Sean Flannery, Prabhakar S Mithal, Erin P Hussey, Eileen T Crehan, Brandon A Ally, Andrew E Budson
Patients with Alzheimer's disease (AD) often exhibit an abnormally liberal response bias in recognition memory tests, responding "old" more frequently than "new." Investigations have shown patients can to shift to a more conservative response bias when given instructions. We examined if patients with mild AD could alter their response patterns when the ratio of old items is manipulated without explicit instruction. Healthy older adults and AD patients studied lists of words and then were tested in three old/new ratio conditions (30%, 50%, or 70% old items)...
September 16, 2017: Brain and Cognition
https://www.readbyqxmd.com/read/28926578/tet-mediated-dna-hydroxymethylation-regulates-retinal-neurogenesis-by-modulating-cell-extrinsic-signaling-pathways
#6
Pawat Seritrakul, Jeffrey M Gross
DNA hydroxymethylation has recently been shown to play critical roles in regulating gene expression and terminal differentiation events in a variety of developmental contexts. However, little is known about its function during eye development. Methylcytosine dioxygenases of the Tet family convert 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), an epigenetic mark thought to serve as a precursor for DNA demethylation and as a stable mark in neurons. Here, we report a requirement for Tet activity during zebrafish retinal neurogenesis...
September 19, 2017: PLoS Genetics
https://www.readbyqxmd.com/read/28926321/evaluation-of-platelet-activation-during-storage-in-leukocyte-depleted-platelet-concentrates
#7
Miodrag Vucic, Zoran Stanojkovic, Ana Antic, Jelana Vucic, Voja Pavlovic
Structural and functional changes in platelets during storage can lead to the loss of platelet reactivity and response. Our aim was to evaluate leukocyte-depleted platelet concentrates, obtained by in-line filtration on storage days 0, 3 and 5. In non-filtered platelet concentrates (NF-PC) group, 180 whole blood units were collected with quadruple blood bags and then compared to another group of 180 whole blood units (leukocyte-depleted platelet concentrates [LD-PC]), collected in Imuflex Whole Blood Filter Saving Platelets (WB-SP) bags with an integrated leukoreduction filter, with regard to the platelet quality and characteristics...
September 19, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28926223/supramolecular-motors-on-graphite-surface-stabilized-by-charge-states-and-hydrogen-bonds
#8
Kai Sun, Ji-Yong Luo, Xin Zhang, Zhi-Jian Wu, Ying Wang, Hong-Kuan Yuan, Zu-Hong Xiong, Shao-Chun Li, Qi-Kun Xue, Jun-Zhong Wang
Molecular motors are nanoscale machines that convert external energies into controlled mechanical movements. In supramolecular motors, the rotator and stator are held together mechanically, and thus the rotation can be essentially barrier free when molecular conformation is negligible. However, nearly all the supramolecular motors appeared in solutions or host-guest complexes. Surface-mounted supramolecular motors have rarely been addressed, even though they are easily manipulated by external fields. Here we report a surface-mounted supramolecular motor assembled by charge states and hydrogen bonds...
September 19, 2017: ACS Nano
https://www.readbyqxmd.com/read/28926196/a-strategy-for-simultaneous-synthesis-of-methallyl-alcohol-and-diethyl-acetal-with-sn-%C3%AE
#9
Wenda Hu, Yan Wan, Lili Zhu, Xiaojie Cheng, Shaolong Wan, Jingdong Lin, Yong Wang
Herein we report a strategy to simultaneously produce two important chemicals namely Methallyl alcohol (Mol) and diethyl acetal (Dal) from methacrolein (Mal) in ethanol solvent at low temperature with the use of Beta zeolites modified by tin (Sn-β catalysts). All Sn-β catalysts were prepared by the solid-state ion-exchange (SSIE) method, wherein the calcination step was conducted in different gas atmospheres. The one pre-calcined in Ar (Sn-β-Ar) diminishes the number of extra-framework Sn species and instead enables more Sn species exchanged into the framework as isolated tetrahedral Sn (IV), thus enhancing the catalytic activity of Meerwein-Ponndorf-Verley (MPV) reaction...
September 19, 2017: ChemSusChem
https://www.readbyqxmd.com/read/28926167/disentangling-environmental-and-host-sources-of-fungal-endophyte-communities-in-an-experimental-beachgrass-study
#10
Aaron S David, Eric W Seabloom, Georgiana May
Disentangling the ecological factors that contribute to the assembly of the microbial symbiont communities within eukaryotic hosts is an ongoing challenge. Broadly speaking, symbiont propagules arrive either from external sources in the environment or from internal sources within the same host individual. To understand the relative importance of these propagule sources to symbiont community assembly, we characterized symbiotic fungal endophyte communities within the roots of three species of beachgrass in a field experiment...
September 19, 2017: Molecular Ecology
https://www.readbyqxmd.com/read/28926145/a-crispr-reimagining-new-twists-and-turns-of-crispr-beyond-the-genome-engineering-revolution
#11
Robert J Plummer, Yi Guo, Ying Peng
Despite its explosive applications in genome engineering, CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) has been developed into a versatile tool beyond its well-known nuclease function. In this prospect article, we summarize a few exciting "off-label" applications of CRISPR including manipulating DNA sequences, visualizing chromosomal loci in living cells, and modulating transcription and chromatin structures. These novel applications will likely elevate CRISPR tools into yet another level of sophistication and diversity, leading to many more exciting cell biological discoveries...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28926135/a-spatial-approach-to-combatting-wildlife-crime
#12
Sally C Faulkner, Michael C A Stevens, Stephanie S Romañach, Peter A Lindsey, Steven C Le Comber
Poaching can have devastating impacts on animal and plant numbers, and in many countries has reached crisis levels, with illegal hunters employing increasingly sophisticated techniques. Here, we show how geographic profiling - a mathematical technique originally developed in criminology and recently applied to animal foraging and epidemiology - can be adapted for use in investigations of wildlife crime, using data from an eight-year study in Savé Valley Conservancy, Zimbabwe that in total includes more than 10,000 incidents of illegal hunting and the deaths of 6,454 wild animals...
September 19, 2017: Conservation Biology: the Journal of the Society for Conservation Biology
https://www.readbyqxmd.com/read/28925815/examining-the-spatiotemporal-disruption-to-gaze-when-using-a-myoelectric-prosthetic-hand
#13
J V V Parr, S J Vine, N R Harrison, G Wood
The aim of this study was to provide a detailed account of the spatial and temporal disruptions to eye-hand coordination when using a prosthetic hand during a sequential fine motor skill. Twenty-one able-bodied participants performed 15 trials of the picking up coins task derived from the Southampton Hand Assessment Procedure with their anatomic hand and with a prosthesis simulator while wearing eye-tracking equipment. Gaze behavior results revealed that when using the prosthesis, performance detriments were accompanied by significantly greater hand-focused gaze and a significantly longer time to disengage gaze from manipulations to plan upcoming movements...
September 19, 2017: Journal of Motor Behavior
https://www.readbyqxmd.com/read/28925566/raman-spectroscopy-for-accurately-characterising-biomolecular-changes-in-androgen-independent-prostate-cancer-cells
#14
Stella Corsetti, Thomas Rabl, David McGloin, Ghulam Nabi
Metastatic prostate cancer resistant to hormonal manipulation is considered the advanced stage of the disease and leads to most cancer related mortality. With new research focusing on modulating cancer growth, it is essential to understand the biochemical changes in cells that can then be exploited for drug discovery and for improving responsiveness to treatment. Raman spectroscopy has a high chemical specificity and can be used to detect and quantify molecular changes at the cellular level. Collection of large datasets generated from biological samples can be employed to form discriminatory algorithms for detection of subtle and early changes in cancer cells...
September 19, 2017: Journal of Biophotonics
https://www.readbyqxmd.com/read/28925532/the-interplay-between-regulatory-focus-and-temporal-distance-in-the-health-context
#15
Aleksandra Berezowska, Arnout R H Fischer, Hans C M van Trijp
OBJECTIVES: This study identifies how the interaction between temporal distance, regulatory focus, and framing of health outcomes affects individuals' intention to adopt a personalized nutrition service. DESIGN: A 2 (temporal distance: immediate health outcomes vs. delayed health outcomes) × 2 (regulatory focus: prevention vs. promotion) × 2 (health outcome framing: illness prevention vs. health promotion) full-factorial between-subjects design. METHODS: In two experiments with samples of 236 and 242 students, regulatory focus was manipulated by asking participants to describe which academic outcomes they want to either achieve or prevent and how they aim to do this...
September 19, 2017: British Journal of Health Psychology
https://www.readbyqxmd.com/read/28925387/a-vital-sugar-code-for-ricin-toxicity
#16
Jasmin Taubenschmid, Johannes Stadlmann, Markus Jost, Tove Irene Klokk, Cory D Rillahan, Andreas Leibbrandt, Karl Mechtler, James C Paulson, Julian Jude, Johannes Zuber, Kirsten Sandvig, Ulrich Elling, Thorsten Marquardt, Christian Thiel, Christian Koerner, Josef M Penninger
Ricin is one of the most feared bioweapons in the world due to its extreme toxicity and easy access. Since no antidote exists, it is of paramount importance to identify the pathways underlying ricin toxicity. Here, we demonstrate that the Golgi GDP-fucose transporter Slc35c1 and fucosyltransferase Fut9 are key regulators of ricin toxicity. Genetic and pharmacological inhibition of fucosylation renders diverse cell types resistant to ricin via deregulated intracellular trafficking. Importantly, cells from a patient with SLC35C1 deficiency are also resistant to ricin...
September 19, 2017: Cell Research
https://www.readbyqxmd.com/read/28925381/cavity-qed-with-hybrid-nanocircuits-from-atomic-like-physics-to-condensed-matter-phenomena
#17
Audrey Cottet, Matthieu C Dartiailh, Matthieu M Desjardins, Tino Cubaynes, Lauriane C Contamin, Matthieu Delbecq, Jérémie J Viennot, Laure E Bruhat, Benoit Douçot, Takis Kontos
Circuit QED techniques have been instrumental in manipulating and probing with exquisite sensitivity the quantum state of superconducting quantum bits coupled to microwave cavities. Recently, it has become possible to fabricate new devices in which the superconducting quantum bits are replaced by hybrid mesoscopic circuits combining nanoconductors and metallic reservoirs. This mesoscopic QED provides a new experimental playground to study the light-matter interaction in electronic circuits. Here, we present the experimental state of the art of mesoscopic QED and its theoretical description...
September 19, 2017: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/28924746/visual-long-term-memory-is-not-unitary-flexible-storage-of-visual-information-as-features-or-objects-as-a-function-of-affect
#18
Philipp Spachtholz, Christof Kuhbandner
Research has shown that observers store surprisingly highly detailed long-term memory representations of visual objects after only a single viewing. However, the nature of these representations is currently not well understood. In particular, it may be that the nature of such memory representations is not unitary but reflects the flexible operating of two separate memory subsystems: a feature-based subsystem that stores visual experiences in the form of independent features, and an object-based subsystem that stores visual experiences in the form of coherent objects...
September 18, 2017: Cognitive, Affective & Behavioral Neuroscience
https://www.readbyqxmd.com/read/28924697/chiropractic-spinal-manipulation-and-the-risk-for-acute-lumbar-disc-herniation-a-belief-elicitation-study
#19
Cesar A Hincapié, J David Cassidy, Pierre Côté, Y Raja Rampersaud, Alejandro R Jadad, George A Tomlinson
PURPOSE: Chiropractic spinal manipulation treatment (SMT) is common for back pain and has been reported to increase the risk for lumbar disc herniation (LDH), but there is no high quality evidence about this. In the absence of good evidence, clinicians can have knowledge and beliefs about the risk. Our purpose was to determine clinicians' beliefs regarding the risk for acute LDH associated with chiropractic SMT. METHODS: Using a belief elicitation design, 47 clinicians (16 chiropractors, 15 family physicians and 16 spine surgeons) that treat patients with back pain from primary and tertiary care practices were interviewed...
September 18, 2017: European Spine Journal
https://www.readbyqxmd.com/read/28924566/comparison-of-oxyhemoglobin-and-deoxyhemoglobin-signal-reliability-with-and-without-global-mean-removal-for-digit-manipulation-motor-tasks
#20
Swethasri Dravida, Jack Adam Noah, Xian Zhang, Joy Hirsch
Functional near-infrared spectroscopy (fNIRS) could be well suited for clinical use, such as measuring neural activity before and after treatment; however, reliability and specificity of fNIRS signals must be ensured so that differences can be attributed to the intervention. This study compared the test-retest and longitudinal reliability of oxyhemoglobin and deoxyhemoglobin signals before and after spatial filtering. In the test-retest experiment, 14 participants were scanned on 2 days while performing four right-handed digit-manipulation tasks...
January 2018: Neurophotonics
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