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https://www.readbyqxmd.com/read/28431487/design-and-fuzzy-logic-control-of-an-active-wrist-orthosis
#1
Ergin Kilic, Erdi Dogan
People who perform excessive wrist movements throughout the day because of their professions have a higher risk of developing lateral and medial epicondylitis. If proper precautions are not taken against these diseases, serious consequences such as job loss and early retirement can occur. In this study, the design and control of an active wrist orthosis that is mobile, powerful and lightweight is presented as a means to avoid the occurrence and/or for the treatment of repetitive strain injuries in an effective manner...
April 1, 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28430147/suitability-of-strain-gage-sensors-for-integration-into-smart-sport-equipment-a-golf-club-example
#2
Anton Umek, Yuan Zhang, Sašo Tomažič, Anton Kos
Wearable devices and smart sport equipment are being increasingly used in amateur and professional sports. Smart sport equipment employs various sensors for detecting its state and actions. The correct choice of the most appropriate sensor(s) is of paramount importance for efficient and successful operation of sport equipment. When integrated into the sport equipment, ideal sensors are unobstructive, and do not change the functionality of the equipment. The article focuses on experiments for identification and selection of sensors that are suitable for the integration into a golf club with the final goal of their use in real time biofeedback applications...
April 21, 2017: Sensors
https://www.readbyqxmd.com/read/28429374/immune-response-of-highly-conserved-influenza-a-virus-matrix-1-peptides
#3
Neha Lohia, Manoj Baranwal
Influenza vaccine development is considered to be complicated and challenging. Constantly evolving influenza viruses require continuous global monitoring and reformulation of the vaccine strains. Peptide conserved among different strains and subtypes of influenza A virus are well-thought-out to be attractive targets for development of cross protective influenza vaccine based on cellular response. Three highly conserved (> 90%) matrix 1 peptides ILGFVFTLTVPSERGLQRRRF (PM 1), LIRHENRMVLASTTAKA (PM 2) and LQAYQKRMGVQMQR (PM 3) containing multiple T cell epitopes have been assessed for their immunogenic potential in vitro, subjecting peripheral blood mononuclear cells from healthy volunteers to repetitive stimulation of these chemically synthesised peptides and measuring their interferon (IFN)-γ level and proliferation by ELISA (Enzyme-linked immunosorbent assay) and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay, respectively...
April 21, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28421811/characterization-of-ornithobacterium-rhinotracheale-field-isolates-from-hungary
#4
Réka Szabó, Enikő Wehmann, László Makrai, Csaba Nemes, Éva Gyuris, Ákos Thuma, Tibor Magyar
Ornithobacterium rhinotracheale is a widely distributed rod-shaped gram-negative bacterium that infects several avian species including chickens and turkeys. It is associated with respiratory signs, growth retardation, mortality, and reduced egg production thus causing severe economic losses to the poultry industries. In this study, 37 field isolates of O. rhinotracheale, collected from various locations in Hungary between 1997 and 2015, were identified and molecularly characterized by the analysis of partial 16S rRNA gene sequences, enterobacterial repetitive intergenic consensus (ERIC)-PCR and random amplified polymorphic DNA (RAPD) PCR assays with the OPG11, OPH19, and M13 primers...
April 19, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28419868/ciprofloxacin-resistance-in-uropathogenic-escherichia-coli-isolates-causing-community-acquired-urinary-infections-in-bras%C3%A3-lia-brazil
#5
Rafaella Christina Rocha Moreira da Silva, Paulo de Oliveira Martins Júnior, Laura Fernandes Gonçalves, Vicente de Paulo Martins, Ana Beatriz Fabrício de Melo, André Pitondo-Silva, Tatiana Amabile de Campos
OBJECTIVES: Considering the global concern of ciprofloxacin resistance, the aim of this study was to evaluate the characteristics of ciprofloxacin-resistant (CIP-R) Escherichia coli isolated from patients with community-acquired urinary tract infections (UTIs) in Brasília, Brazil. METHODS: CIP-R E. coli isolated from different outpatients between July 2013 and April 2014 in a tertiary hospital were analysed for antibiotic resistance profile, phylotype, uropathogenic E...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28415976/smrt-genome-assembly-corrects-reference-errors-resolving-the-genetic-basis-of-virulence-in-mycobacterium-tuberculosis
#6
Afif Elghraoui, Samuel J Modlin, Faramarz Valafar
BACKGROUND: The genetic basis of virulence in Mycobacterium tuberculosis has been investigated through genome comparisons of virulent (H37Rv) and attenuated (H37Ra) sister strains. Such analysis, however, relies heavily on the accuracy of the sequences. While the H37Rv reference genome has had several corrections to date, that of H37Ra is unmodified since its original publication. RESULTS: Here, we report the assembly and finishing of the H37Ra genome from single-molecule, real-time (SMRT) sequencing...
April 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28409126/genomics-and-comparative-genomic-analyses-provide-insight-into-the-taxonomy-and-pathogenic-potential-of-novel-emmonsia-pathogens
#7
Ying Yang, Qiang Ye, Kang Li, Zongwei Li, Xiaochen Bo, Zhen Li, Yingchun Xu, Shengqi Wang, Peng Wang, Huipeng Chen, Junzhi Wang
Over the last 50 years, newly described species of Emmonsia-like fungi have been implicated globally as sources of systemic human mycosis (emmonsiosis). Their ability to convert into yeast-like cells capable of replication and extra-pulmonary dissemination during the course of infection differentiates them from classical Emmonsia species. Immunocompromised patients are at highest risk of emmonsiosis and exhibit high mortality rates. In order to investigate the molecular basis for pathogenicity of the newly described Emmonsia species, genomic sequencing and comparative genomic analyses of Emmonsia sp...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28399998/bacterial-ecology-of-artisanal-minas-cheeses-assessed-by-culture-dependent-and-independent-methods
#8
Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti
Artisanal Minas cheese is produced in Minas Gerais state, Brazil and its varieties are named according to their geographical origin (Serro, Canastra, Serra do Salitre, Araxá and Campo das Vertentes). The cheese is produced with raw cow's milk and the whey from the previous cheese production ("pingo"). The high economic and cultural importance of artisanal cheese in Brazil justifies the efforts to ensure its safety, quality and provenance. This study aimed to characterize the microbial diversity composition, and geographical distribution of artisanal Minas cheese, focusing on the characterization of its autochthonous lactic acid bacteria (LAB) microbiota...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28391652/investigation-on-the-incidence-of-adverse-reactions-viraemia-and-haematological-changes-following-field-immunization-of-cattle-using-a-live-attenuated-vaccine-against-lumpy-skin-disease
#9
P-D Katsoulos, S C Chaintoutis, C I Dovas, Z S Polizopoulou, G D Brellou, E I Agianniotaki, K E Tasioudi, E Chondrokouki, O Papadopoulos, H Karatzias, C Boscos
The present study was performed to investigate the clinical impact and certain virological and haematological parameters following immunization of cattle against lumpy skin disease (LSD). The study was conducted in a dairy cattle farm (215 animals), immunized with a Neethling strain-based live vaccine. Twenty-seven animals (14 lactating cows, four dry cows and nine calves) were randomly selected for repetitive blood and saliva samplings. An EvaGreen-based real-time PCR was designed to differentiate vaccine from field LSDVs...
April 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28385004/molecular-and-genomic-features-of-mycobacterium-bovis-strain-1595-isolated-from-korean-cattle
#10
Narae Kim, Yunho Jang, Jin Kyoung Kim, Soyoon Ryoo, Ka Hee Kwon, Miso Kim, Shin Seok Kang, Hyeon Seop Byeon, Hee Soo Lee, Young-Hee Lim, Jae-Myung Kim
The aim of this study was to investigate the molecular characteristics and conduct a comparative genomic analysis for Mycobacterium bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined using Illumina MiSeq and PacBio single-molecule real-time technology, which showed a genome of 4,351,712 bp in size with a 65...
April 6, 2017: Journal of Veterinary Science
https://www.readbyqxmd.com/read/28378509/hamstring-to-quadriceps-fatigue-ratio-offers-new-and-different-muscle-function-information-than-the-conventional-non-fatigued-ratio
#11
Matheus Daros Pinto, Anthony J Blazevich, Lars L Andersen, Pedro Mil-Homens, Ronei Silveira Pinto
Commonly used injury risk prediction tests such as the hamstring-to-quadriceps (H:Q) strength ratio appear to be poor predictors of non-contact injury. However, these tests are typically performed in a non-fatigued state, despite accumulated fatigue being an important risk factor for both hamstring strain (HS) and anterior cruciate ligament (ACL) injuries in professional soccer players PURPOSE: To examine the influence of neuromuscular fatigue on the H:Q strength ratio and the association between fatigued and non-fatigued ratio scores, after the effect of different H:Q calculation methods were compared and contrasted METHODS: Thirty-five professional soccer players performed a 30-repetition isokinetic fatigue test protocol...
April 4, 2017: Scandinavian Journal of Medicine & Science in Sports
https://www.readbyqxmd.com/read/28373764/effect-of-treatment-failure-on-the-caga-epiya-motif-in-helicobacter-pylori-strains-from-colombian-subjects
#12
Javier Andres Bustamante-Rengifo, Andres Jenuer Matta, Alvaro Jairo Pazos, Luis Eduardo Bravo
AIM: To evaluate effect of treatment failure on cagA and vacA genotypes in Helicobacter pylori (H. pylori) isolates from Colombia. METHODS: One hundred and seventy-six participants infected with H. pylori from Colombia were treated during 14 d with the triple-standard therapy. Six weeks later, eradication was evaluated by (13)C-Urea breath test. Patients with treatment failure were subjected to endoscopy control; biopsies obtained were used for histopathology and culture...
March 21, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28368855/seroprevalence-and-molecular-epidemiology-of-east1-gene-carrying-escherichia-coli-from-diarrheal-patients-and-raw-meats
#13
Kannika Sukkua, Somruthai Manothong, Pharanai Sukhumungoon
INTRODUCTION: Several Escherichia coli pathotypes have been reported in Thailand; however, information on enteroaggregative heat-stable enterotoxin 1 (EAST1)-carrying E. coli (EAST1-EC) is insufficient. Previous reports show that consumption of raw meats causes diarrheagenic E. coli infections. In this study, we investigated the seroprevalence and genetic relationship of EAST1-EC from clinical and raw meat samples. METHODOLOGY: Diarrheal patients and raw meat samples were investigated for the presence of EAST1-EC by performing polymerase chain reaction (PCR) to detect astA...
March 31, 2017: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28361238/a-novel-genetic-tool-for-metabolic-optimization-of-corynebacterium-glutamicum-efficient-and-repetitive-chromosomal-integration-of-synthetic-promoter-driven-expression-libraries
#14
Jing Shen, Jun Chen, Peter Ruhdal Jensen, Christian Solem
Fine-tuning the expression level of multiple genes is usually pivotal for metabolic optimization. We have developed a tool for this purpose for the important industrial workhorse Corynebacterium glutamicum that allows for the introduction of synthetic promoter-driven expression libraries of arbitrary genes. We first devised a method for introducing genetic elements into the chromosome repeatedly, relying on site-specific recombinases and the vector pJS31 serving as the carrier. The pJS31 vector contains a synthetic cassette including a phage attachment site attP for integration, a bacterial attachment site attB for subsequent integration, a multiple cloning site, and two modified loxP sites to facilitate easy removal of undesirable vector elements...
March 30, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28360895/genetic-diversity-of-brucella-reference-and-non-reference-phages-and-its-impact-on-brucella-typing
#15
Jens A Hammerl, Cornelia Göllner, Claudia Jäckel, Holger C Scholz, Karsten Nöckler, Jochen Reetz, Sascha Al Dahouk, Stefan Hertwig
Virulent phages have been used for many years to type Brucella isolates, but until recently knowledge about the genetic makeup of these phages remains limited. In this work the host specificity and genomic sequences of the original set (deposited in 1960) of VLA Brucella reference phages Tb, Fi, Wb, Bk2, R/C, and Iz were analyzed and compared with hitherto described brucellaphages. VLA phages turned out to be different from homonymous phages in other laboratories. The host range of the phages was defined by performing plaque assays with a wide selection of Brucella strains...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28356066/effectiveness-of-conservative-interventions-for-sickness-and-pain-behaviors-induced-by-a-high-repetition-high-force-upper-extremity-task
#16
D L Xin, J Hadrévi, M E Elliott, M Amin, M Y Harris, A E Barr-Gillespie, M F Barbe
BACKGROUND: Systemic inflammation is known to induce sickness behaviors, including decreased social interaction and pain. We have reported increased serum inflammatory cytokines in a rat model of repetitive strain injury (rats perform an upper extremity reaching task for prolonged periods). Here, we sought to determine if sickness behaviors are induced in this model and the effectiveness of conservative treatments. METHODS: Experimental rats underwent initial training to learn a high force reaching task (10 min/day, 5 days/week for 6 weeks), with or without ibuprofen treatment (TRHF vs...
March 29, 2017: BMC Neuroscience
https://www.readbyqxmd.com/read/28351427/use-of-whole-genome-sequencing-to-distinguish-relapse-from-reinfection-in-a-completed-tuberculosis-clinical-trial
#17
Adam A Witney, Anna L E Bateson, Amina Jindani, Patrick P J Phillips, David Coleman, Neil G Stoker, Philip D Butcher, Timothy D McHugh
BACKGROUND: RIFAQUIN was a tuberculosis chemotherapy trial in southern Africa including regimens with high-dose rifapentine with moxifloxacin. Here, the application of whole-genome sequencing (WGS) is evaluated within RIFAQUIN for identifying new infections in treated patients as either relapses or reinfections. WGS is further compared with mycobacterial interspersed repetitive units-variable number tandem repeats (MIRU-VNTR) typing. This is the first report of WGS being used to evaluate new infections in a completed clinical trial for which all treatment and epidemiological data are available for analysis...
March 29, 2017: BMC Medicine
https://www.readbyqxmd.com/read/28348872/phase-variable-dna-repeats-in-neisseria-gonorrhoeae-influence-transcription-translation-and-protein-sequence-variation
#18
Marta A Zelewska, Madhuri Pulijala, Russell Spencer-Smith, Hiba-Tun-Noor A Mahmood, Billie Norman, Colin P Churchward, Alan Calder, Lori A S Snyder
There are many types of repeated DNA sequences in the genomes of the species of the genus Neisseria, from homopolymeric tracts to tandem repeats of hundreds of bases. Some of these have roles in the phase-variable expression of genes. When a repeat mediates phase variation, reversible switching between tract lengths occurs, which in the species of the genus Neisseria most often causes the gene to switch between on and off states through frame shifting of the open reading frame. Changes in repeat tract lengths may also influence the strength of transcription from a promoter...
August 2016: Microbial Genomics
https://www.readbyqxmd.com/read/28345184/spatial-molecular-epidemiology-of-carbapenem-resistant-and-new-delhi-metallo-beta-lactamase-blandm-producing-escherichia-coli-strains-from-farmed-piglets-of-india
#19
B S Pruthvishree, O R Vinodh Kumar, D K Sinha, Y P S Malik, Z Dubal, P A Desingu, M Shivakumar, Narayanan Krishnaswamy, B R Singh
AIM: A cross-sectional study was conducted in ten government organized pig farms between 2014 and 2016 representing seven states of India to understand the epidemiology of carbapenem resistance in Escherichia coli isolates. METHODS AND RESULTS: In this study, 673 fecal samples both from non- diarhhoeic (n=501) and diarrhoeic (n=172) piglets were processed for isolation of carbapenem resistant E. coli. Wherein, 112 confirmed E. coli isolates were further subjected for genotypic confirmation of carbapenem resistance and virulence factors...
March 27, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28338020/epigenetic-regulation-of-intronic-transgenes-in-arabidopsis
#20
Kenji Osabe, Yoshiko Harukawa, Saori Miura, Hidetoshi Saze
Defense mechanisms of plant genomes can epigenetically inactivate repetitive sequences and exogenous transgenes. Loss of mutant phenotypes in intronic T-DNA insertion lines by interaction with another T-DNA locus, termed T-DNA suppression, has been observed in Arabidopsis thaliana, although the molecular basis of establishment and maintenance of T-DNA suppression is poorly understood. Here we show that maintenance of T-DNA suppression requires heterochromatinisation of T-DNA sequences and the nuclear proteins, INCREASED IN BONSAI METHYLATION 2 (IBM2) and ENHANCED DOWNY MILDEW 2 (EDM2), which prevent ectopic 3' end processing of mRNA in atypically long introns containing T-DNA sequences...
March 24, 2017: Scientific Reports
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