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https://www.readbyqxmd.com/read/28301296/distinct-leishmania-infantum-strains-circulate-in-humans-and-dogs-in-the-emilia-romagna-region-northeastern-italy
#1
Gianluca Rugna, Elena Carra, Francesco Corpus, Mattia Calzolari, Daniela Salvatore, Romeo Bellini, Antonietta Di Francesco, Erica Franceschini, Antonella Bruno, Giovanni Poglayen, Stefania Varani, Fabrizio Vitale, Giuseppe Merialdi
Human leishmaniasis is an emerging problem in Italy and is on the increase in the Emilia-Romagna region, northeastern part of the country. Nevertheless, studies dealing with the molecular characterization of Leishmania spp. circulating in these areas are limited. In the present work, we explored the genetic polymorphism of Leishmania isolates from 28 cases of canine leishmaniasis and three cases of human visceral leishmaniasis (VL), which occurred in 2013-2014 in the Emilia-Romagna region. The characterization was carried out in comparison with nine human isolates of Leishmania from other VL endemic Italian regions and two reference strains...
March 16, 2017: Vector Borne and Zoonotic Diseases
https://www.readbyqxmd.com/read/28295043/chlorin-e6-mediated-photodynamic-inactivation-for-multidrug-resistant-pseudomonas-aeruginosa-keratitis-in-mice-in-vivo
#2
Ming-Feng Wu, Mona Deichelbohrer, Thomas Tschernig, Matthias W Laschke, Nóra Szentmáry, Dirk Hüttenberger, Hans-Jochen Foth, Berthold Seitz, Markus Bischoff
Following corneal epithelium scratches, mouse corneas were infected with the multidrug resistant (MDR) P. aeruginosa strain PA54. 24 hours later, 0% (for control group), 0.01%, 0.05% or 0.1% Chlorin e6 (Ce6), a second generation photosensitizer derived from chlorophyll, was combined with red light, for photodynamic inactivation (PDI). 1 hour or 2 days later, entire mouse eyes were enucleated and homogenized for counting colony forming units (CFU) of P. aeruginosa. For comparison, 0.1% Ce6 mediated PDI was started at 12 hours post infection, and 0...
March 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28291671/molecular-characterization-of-kpc-producing-klebsiella-pneumoniae-isolated-from-patients-in-a-public-hospital-in-caracas-venezuela
#3
Aura Dayana Falco Restrepo, Mariel Alexandra Velásquez Nieves, Howard Takiff
INTRODUCTION: Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are amongst the most important causative agents of nosocomial infections worldwide. Isolates of this bacterium have been identified in Venezuela but little is known about their local spread. The aim of this study was to perform the molecular characterization of KPC-producing strains isolated from 2012 to 2013 in a public hospital in Caracas, Venezuela. METHODS: Twenty-two K. pneumoniae clinical isolates phenotypically classified as KPC producing were subjected to PCR screening for the presence of blaKPC genes and their location within transposon Tn4401...
March 11, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28291648/combining-stable-insect-cell-lines-with-baculovirus-mediated-expression-for-multi-ha-influenza-vlp-production
#4
Daniela P Sequeira, Ricardo Correia, Manuel J T Carrondo, António Roldão, Ana P Teixeira, Paula M Alves
Safer and broadly protective vaccines are needed to cope with the continuous evolution of circulating influenza virus strains and promising approaches based on the expression of multiple hemagglutinins (HA) in a virus-like particle (VLP) have been proposed. However, expression of multiple genes in the same vector can lead to its instability due to tandem repetition of similar sequences. By combining stable with transient expression systems we can rationally distribute the number of genes to be expressed per platform and thus mitigate this risk...
March 10, 2017: Vaccine
https://www.readbyqxmd.com/read/28249607/molecular-typing-of-mycobacterium-tuberculosis-complex-isolated-from-pulmonary-tuberculosis-patients-in-central-ethiopia
#5
Zufan Bedewi, Adane Worku, Yalemtsehay Mekonnen, Getnet Yimer, Girmay Medhin, Gezahegne Mamo, Rembert Pieper, Gobena Ameni
BACKGROUND: Identification of the types of strains of Mycobacterium tuberculosis (M. tuberculosis) complex causing tuberculosis (TB) could contribute to TB control program of specific geographic region as well as it could add knowledge onto the existing literature on TB worldwide. The objective of the present study was to identify the species and strains of M. tuberculosis complex causing pulmonary tuberculosis in central Ethiopia. METHODS: A health institution- based cross-sectional study was conducted on 338 smear positive TB cases visiting three hospitals between October 2012 and September 2013...
March 1, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28220547/identification-and-molecular-epidemiology-of-dermatophyte-isolates-by-repetitive-sequence-pcr-based-dna-fingerprinting-using-the-diversilab-system-in-turkey
#6
A Nedret Koc, Mustafa A Atalay, Melek Inci, Fatma M Sariguzel, Hafize Sav
Dermatophyte species, isolation and identification in clinical samples are still difficult and take a long time. The identification and molecular epidemiology of dermatophytes commonly isolated in a clinical laboratory in Turkey by repetitive sequence-based PCR (rep-PCR) were assessed by comparing the results with those of reference identification. A total of 44 dermatophytes isolated from various clinical specimens of 20 patients with superficial mycoses in Kayseri and 24 patients in Hatay were studied. The identification of dermatophyte isolates was based on the reference identification and rep-PCR using the DiversiLab System (BioMerieux)...
February 21, 2017: Mycoses
https://www.readbyqxmd.com/read/28201995/zoonotic-necrotizing-myositis-caused-by-streptococcus-equi-subsp-zooepidemicus-in-a-farmer
#7
Bård Reiakvam Kittang, Veronika Kuchařová Pettersen, Oddvar Oppegaard, Dag Harald Skutlaberg, Håvard Dale, Harald G Wiker, Steinar Skrede
BACKGROUND: Streptococcus equi subsp. zooepidemicus is a beta-hemolytic group C streptococcus mainly causing infections in domesticated animals. Here we describe the first case of zoonotic necrotizing myositis caused by this bacterium. CASE PRESENTATION: The patient was a 73-year-old, previously healthy farmer with two asymptomatic Shetland ponies in his stable. After close contact with the ponies while feeding them, he rapidly developed erythema of his left thigh and sepsis with multiple organ failure...
February 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28195658/risk-of-work-injury-among-adolescent-students-from-single-and-partnered-parent-families
#8
Imelda S Wong, F Curtis Breslin
BACKGROUND: Parental involvement in keeping their children safe at work has been examined in a handful of studies, with mixed results. Evidence has suggested that non-work injury risk is higher among children from single-parent families, but little is known about their risk for work-related injuries. METHODS: Five survey cycles of the Canadian Community Health Survey were pooled to create a nationally representative sample of employed 15-19-year old students (N = 16,620)...
March 2017: American Journal of Industrial Medicine
https://www.readbyqxmd.com/read/28195345/in-vivo-measurements-of-human-neck-skin-elasticity-using-mri-and-finite-element-modeling
#9
Yunqiang An, Changjin Ji, Yong Li, Jianxia Wang, Xinyue Zhang, Yaqi Huang
PURPOSE: The assessment of mechanical properties of the human skin is very important in investigating the mechanism of obstructive sleep apnea, a common disorder characterized by repetitive collapse and obstruction of the upper airway during sleep. In this study, a unique method, combining magnetic resonance imaging (MRI) and finite element modeling (FEM), was developed to obtain the value of the in vivo elastic modulus of the neck skin. MEHTHOD: A total of 22 subjects, 16 males and 6 females, were recruited to participate in the MRI studies...
February 13, 2017: Medical Physics
https://www.readbyqxmd.com/read/28191309/co-existence-of-blaoxa-23-and-blandm-1-genes-of-acinetobacter-baumannii-isolated-from-nepal-antimicrobial-resistance-and-clinical-significance
#10
Prabhu Raj Joshi, Mahesh Acharya, Trishna Kakshapati, Udomluk Leungtongkam, Rapee Thummeepak, Sutthirat Sitthisak
BACKGROUND: Molecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. METHODS: A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and blaOXA-51 genes...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28182316/tissue-mechanics-during-temporal-summation-of-sequentially-cuff-pressure-induced-pain-in-healthy-volunteers-and-patients-with-painful-osteoarthritis
#11
B Manafi-Khanian, K Kjaer Petersen, L Arendt-Nielsen
BACKGROUND: The phenomenon of temporal summation to repetitive pressure pain stimuli is an important central neural mechanism for pain intensity encoding. This study evaluated the time-dependent behaviour of mechanical characteristics of soft tissue during repeated cuff stimulation used for eliciting temporal summation of cuff pressure-evoked pain. Such information of tissue mechanics is important for the interpretation of the pain response evoked during sequential stimulations. METHODS: Temporal summation was assessed in 16 subjects separated into two groups (healthy controls and severe knee osteoarthritis patients) using a visual analogue scale during 10 repetitive painful cuff stimuli (1-s duration, 1-s break) of the lower leg...
February 9, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/28168080/the-femoral-neck-mechanoresponse-to-hip-extensors-exercise-a-case-study
#12
Saulo Martelli, Hossein Mokhtarzadeh, Peter Pivonka, Peter R Ebeling
Physical activity is recommended to prevent age-related bone loss. However, the proximal femur mechanoresponse is variable, possibly because of a muscle-dependant mechanoresponse. We compared the proximal femur response with the femoral strain pattern generated by the hip extensor muscles. A healthy participant underwent a six-month unilateral training of the hip extensor muscles using a resistance weight regularly adjusted to the 80% of the one-repetition maximum weight. DXA-based measurements of the areal Bone Mineral Density (aBMD) in the exercise leg were adjusted for changes in the control leg...
2017: Journal of Osteoporosis
https://www.readbyqxmd.com/read/28167525/the-history-of-bordetella-pertussis-genome-evolution-includes-structural-rearrangement
#13
Michael R Weigand, Yanhui Peng, Vladimir Loparev, Dhwani Batra, Katherine E Bowden, Mark Burroughs, Pamela K Cassiday, Jamie K Davis, Taccara Johnson, Phalasy Juieng, Kristen Knipe, Marsenia H Mathis, Andrea M Pruitt, Lori Rowe, Mili Sheth, M Lucia Tondella, Margaret M Williams
Despite high pertussis vaccine coverage, reported cases of whooping cough (pertussis) have increased over the last decade in the United States and other developed countries. Although Bordetella pertussis is well known for its limited gene sequence variation, recent advances in long-read sequencing technology have begun to reveal genome structural heterogeneity among otherwise indistinguishable isolates, even within geographically or temporally defined epidemics. We have compared rearrangements among complete genome assemblies from 257 B...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28166858/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miru-vntr
#14
B R Imperiale, R D Moyano, A B DI Giulio, M A Romero, M F Alvarado Pinedo, M P Santangelo, G E Travería, N S Morcillo, M I Romano
Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP...
February 7, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28166854/an-outbreak-of-burkholderia-cepacia-complex-infections-associated-with-contaminated-liquid-docusate
#15
Lucila Marquez, Katie N Jones, Elaine M Whaley, Tjin H Koy, Paula A Revell, Ruston S Taylor, M Brooke Bernhardt, Jeffrey L Wagner, James J Dunn, John J LiPuma, Judith R Campbell
OBJECTIVE To investigate an outbreak of Burkholderia cepacia complex and describe the measures that revealed the source. SETTING A 629-bed, tertiary-care, pediatric hospital in Houston, Texas. PATIENTS Pediatric patients without cystic fibrosis (CF) hospitalized in the pediatric and cardiovascular intensive care units. METHODS We investigated an outbreak of B. cepacia complex from February through July 2016. Isolates were evaluated for molecular relatedness with repetitive extragenic palindromic polymerase chain reaction (rep-PCR); specific species identification and genotyping were performed at an independent laboratory...
February 7, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28150578/resistotyping-phenotyping-and-genotyping-of-new-delhi-metallo-%C3%AE-lactamase-ndm-among-gram-negative-bacilli-from-iranian-patients
#16
Dariush Shokri, Mohammad Rabbani Khorasgani, Seyed Masih Fatemi, Abbas Soleimani-Delfan
PURPOSE: The purpose of this study was investigation of New Delhi metallo-β-lactamase (NDM) production among gram negative bacilli. METHODOLOGY: Antibiogram-Resistotyping and detection of NDM in clinical isolates of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa and comparative evaluation of the diagnostic performance of three phenotypic methods for NDM detection considering PCR as gold standard were performed. Minimum Inhibitory Concentration (MIC) of antibiotics against NDM positive strains by E-test and clonal relationship analysis using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) in these strains were determined...
February 1, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28149918/multiple-large-clusters-of-tuberculosis-in-london-a-cross-sectional-analysis-of-molecular-and-spatial-data
#17
Catherine M Smith, Helen Maguire, Charlotte Anderson, Neil Macdonald, Andrew C Hayward
Large outbreaks of tuberculosis (TB) represent a particular threat to disease control because they reflect multiple instances of active transmission. The extent to which long chains of transmission contribute to high TB incidence in London is unknown. We aimed to estimate the contribution of large clusters to the burden of TB in London and identify risk factors. We identified TB patients resident in London notified between 2010 and 2014, and used 24-locus mycobacterial interspersed repetitive units-variable number tandem repeat strain typing data to classify cases according to molecular cluster size...
January 2017: ERJ Open Research
https://www.readbyqxmd.com/read/28132109/bridging-with-reduced-overlap-fixation-and-peritoneal-grip-can-prevent-slippage-of-dis-class-a-meshes
#18
F Kallinowski, F Harder, T G Silva, A Mahn, M Vollmer
PURPOSE: Ventral hernia repair can be performed safely using meshes which are primarily stable upon dynamic intermittent straining (DIS) at recommended overlap. In specific clinical situations, e.g., at bony edges, bridging of the hernial orifice with reduced overlap might be necessary. To gain insight into the durability of various applications, two different meshes with the best tissue grip known so far were assessed. METHODS: The model uses dynamic intermittent strain and comprises the repetition of submaximal impacts delivered via a hydraulically driven plastic containment...
January 28, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28127718/detection-of-toxoplasma-gondii-in-faeces-of-privately-owned-cats-using-two-pcr-assays-targeting-the-b1-gene-and-the-529-bp-repetitive-element
#19
Fabrizia Veronesi, Azzurra Santoro, Giovanni L Milardi, Manuela Diaferia, Giulia Morganti, David Ranucci, Simona Gabrielli
Toxoplasma gondii infection is a worldwide parasitic zoonosis with a high-health risk for humans. The key epidemiological role played by felids is related to oocyst shedding. The present study compared two amplification protocols for the molecular diagnosis of Toxoplasma infection in owned cats. A total of 78 owned cats referred to an Italian university-teaching hospital and exposed to various T. gondii-associated risk factors were sampled for blood and faeces. Faecal specimens were processed by flotation and tested using 2 copro-PCRs targeting the widely used B1 gene and the 529-bp repetitive element (RE)...
January 27, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28127419/towards-long-read-metagenomics-complete-assembly-of-three-novel-genomes-from-bacteria-dependent-on-a-diazotrophic-cyanobacterium-in-a-freshwater-lake-co-culture
#20
Connor B Driscoll, Timothy G Otten, Nathan M Brown, Theo W Dreher
Here we report three complete bacterial genome assemblies from a PacBio shotgun metagenome of a co-culture from Upper Klamath Lake, OR. Genome annotations and culture conditions indicate these bacteria are dependent on carbon and nitrogen fixation from the cyanobacterium Aphanizomenon flos-aquae, whose genome was assembled to draft-quality. Due to their taxonomic novelty relative to previously sequenced bacteria, we have temporarily designated these bacteria as incertae sedis Hyphomonadaceae strain UKL13-1 (3,501,508 bp and 56...
2017: Standards in Genomic Sciences
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