keyword
MENU ▼
Read by QxMD icon Read
search

Repetitive strain

keyword
https://www.readbyqxmd.com/read/28100602/find-tuberculosis-strain-bank-a-resource-for-researchers-and-developers-working-on-tests-to-detect-mycobacterium-tuberculosis-and-related-drug-resistance
#1
Belay Tessema, Pamela Nabeta, Eloise Valli, Audrey Albertini, Jimena Collantes, Nguyen Huu Lan, Elena Romancenco, Nestani Tukavdze, Claudia M Denkinger, David L Dolinger
BACKGROUND: The spread of multidrug-resistant tuberculosis and extensively drug-resistant TB hampers the global efforts in the fight against tuberculosis. To enhance the development and evaluation of diagnostic tests quickly and efficiently, well characterized strains and samples from drug resistant tuberculosis patients are necessary. In this project, FIND has focused on the collection, characterization and storage of such well-characterized reference materials and to make them available to researchers and developers...
January 18, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28099994/the-impact-of-primer-sets-on-detection-of-the-gene-encoding-biofilm-associated-protein-bap-in-acinetobacter-baumannii-in-silico-and-in-vitro-analysis
#2
Mansoor Kodori, Masoumeh Douraghi, Mehdi Yaseri, Mohammad Rahbar
The Acinetobacter baumannii virulence protein Bap is encoded by a large gene and contains both variable sequence and repetitive modules. To date, four primer sets targeting different regions of bap have been designed, but no study has evaluated all these primers simultaneously for detection of bap. Here, we assessed the effect of primer sets Bap I to IV, on detection of bap both in silico and in vitro. Using the primer set Bap II, all 143 tested strains yielded an amplicon corresponding to bap gene. This primer set showed the highest sensitivity (100%, 95% CI: 97...
January 18, 2017: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/28096975/a-prospective-survey-of-pseudomonas-aeruginosa-colonization-and-infection-in-the-intensive-care-unit
#3
Regev Cohen, Frida Babushkin, Shoshana Cohen, Marina Afraimov, Maurice Shapiro, Martina Uda, Efrat Khabra, Amos Adler, Ronen Ben Ami, Svetlana Paikin
BACKGROUND: Pseudomonas aeruginosa (PA) surveillance may improve empiric antimicrobial therapy, since colonizing strains frequently cause infections. This colonization may be 'endogenous' or 'exogenous', and the source determines infection control measures. We prospectively investigated the sources of PA, the clinical impact of PA colonization upon admission and the dynamics of colonization at different body sites throughout the intensive care unit stay. METHODS: Intensive care patients were screened on admission and weekly from the pharynx, endotracheal aspirate, rectum and urine...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28087673/rdna-stability-is-supported-by-many-buffer-genes-introduction-to-the-yeast-rdna-stability-database
#4
Takehiko Kobayashi, Mariko Sasaki
The ribosomal RNA gene (rDNA) is the most abundant gene in yeast and other eukaryotic organisms. Due to its heavy transcription, repetitive structure and programmed replication fork pauses, the rDNA is one of the most unstable regions in the genome. Thus, the rDNA is the best region to study the mechanisms responsible for maintaining genome integrity. Recently, we screened a library of ∼4,800 budding yeast gene knockout strains to identify mutants defective in the maintenance of rDNA stability. The results of this screen are summarized in the Yeast rDNA Stability Database, in which the stability and copy number of rDNA in each mutant are presented...
January 12, 2017: FEMS Yeast Research
https://www.readbyqxmd.com/read/28079648/acute-response-of-the-infraspinatus-and-biceps-tendons-to-pitching-in-youth-baseball
#5
Adam Popchak, Nathan Hogaboom, Dharmesh Vyas, John Abt, Anthony Delitto, James J Irrgang, Michael Boninger
PURPOSE: Youth baseball frequently results in repetitive strain injuries. Quantitative ultrasound allows real-time imaging with the ability to identify acute markers of tendon change. The study objective was to determine acute quantitative ultrasound changes in the long head of the biceps and infraspinatus tendons of the throwing and non-throwing shoulders during a pitching performance. We hypothesized the tendons of the pitching arm would exhibit an increased width and decreased echogenicity after pitching and that tendons of the non-pitching arm would not demonstrate such changes...
January 11, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28069063/the-ribosomal-transcription-units-of-haplorchis-pumilio-and-h-taichui-and-the-use-of-28s-rdna-sequences-for-phylogenetic-identification-of-common-heterophyids-in-vietnam
#6
Thanh Hoa Le, Khue Thi Nguyen, Nga Thi Bich Nguyen, Huong Thi Thanh Doan, Do Trung Dung, David Blair
BACKGROUND: Heterophyidiasis is now a major public health threat in many tropical countries. Species in the trematode family Heterophyidae infecting humans include Centrocestus formosanus, Haplorchis pumilio, H. taichui, H. yokogawai, Procerovum varium and Stellantchasmus falcatus. For molecular phylogenetic and systematic studies on trematodes, we need more prospective markers for taxonomic identification and classification. This study provides near-complete ribosomal transcription units (rTU) from Haplorchis pumilio and H...
January 9, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28061633/mechanisms-of-bone-response-to-injury
#7
Keren E Dittmer, Elwyn C Firth
Bone, despite its relatively inert appearance, is a tissue that is capable of adapting to its environment. Wolff's law, first described in the 19th century, describes the ability of bone to change structure depending on the mechanical forces applied to it. The mechanostat model extended this principle and suggested that the amount of strain a bone detects depends on bone strength and the amount of muscle force applied to the bone. Experimental studies have found that low-magnitude, high-frequency mechanical loading is considered to be the most effective at increasing bone formation...
January 1, 2017: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/28056788/genotype-variation-and-genetic-relationship-among-escherichia-coli-from-nursery-pigs-located-in-different-pens-in-the-same-farm
#8
Ana Herrero-Fresno, Shahana Ahmed, Monica Hegstad Hansen, Matthew Denwood, Camilla Zachariasen, John Elmerdahl Olsen
BACKGROUND: So far, little is known about the genetic diversity and relatedness among Escherichia coli (E. coli) populations in the gut of swine. Information on this is required to improve modeling studies on antimicrobial resistance aiming to fight its occurrence and development. This work evaluated the genotype variation of E. coli isolated from swine fecal samples at the single pig and pen level, as well as between pens using repetitive extragenic palindromic (REP) PCR fingerprinting and pulsed field gel electrophoresis (PFGE)...
January 5, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28053382/a-novel-adjustable-dynamic-plate-for-treatment-of-long-bone-fractures-an-in-vitro-biomechanical-study
#9
Ahmet Karakasli, Nihat Acar, Ahmet Karaarslan, Fatih Ertem, Hasan Havitcioglu
INTRODUCTION: Locking compression plate (LCP) system was designed to provide bone stability and to enhance bone healing. However, implant failure, nonunion and instability are still frequently encountered complications. The purpose of this study was to assess and compare the biomechanical characteristics of a novel adjustable dynamic plate (ADP) with the commonly used LCP. MATERIALS AND METHODS: Twelve 4th generation composite artificial femoral bones were used...
October 2016: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/28053217/high-interlaboratory-reproducibility-and-accuracy-of-next-generation-sequencing-based-bacterial-genotyping-in-a-ring-trial
#10
Alexander Mellmann, Paal Skytt Andersen, Stefan Bletz, Alexander W Friedrich, Thomas A Kohl, Berit Lilje, Stefan Niemann, Karola Prior, John W Rossen, Dag Harmsen
Today, next-generation whole genome sequencing (WGS) technology is increasingly used to determine the genetic relationship of bacteria on a nearly whole genome level for infection control purposes and molecular surveillance. Here, we conducted a multicenter ring-trial comprising five laboratories to determine the reproducibility and accuracy of WGS-based typing.The participating laboratories sequenced 20 blind-coded Staphylococcus aureus DNA samples using the 250 bp paired-end chemistry for library preparation in a single sequencing run on an Illumina Miseq sequencer...
January 4, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28043599/genetic-diversity-transmission-dynamics-and-drug-resistance-of-mycobacterium-tuberculosis-in-luanda-angola
#11
João Perdigão, Sofia Clemente, Jorge Ramos, Pedro Masakidi, Diana Machado, Carla Silva, Isabel Couto, Miguel Viveiros, Nuno Taveira, Isabel Portugal
OBJECTIVE/BACKGROUND: Despite the important role that the African region plays in a global tuberculosis (TB) epidemiological context, many countries in the region still lack data on the prevalence of specific Mycobacterium tuberculosis strains and drug resistance. This is the case for Angola, which presently lacks any data concerning drug-resistance rates and prevalence of specific M. tuberculosis genotypes and respective population structure. In this study, we made the first characterization of the genetic diversity and drug resistance of M...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043547/restriction-fragment-length-polymorphism-pattern-of-mycobacterium-isolates-from-rodents-in-infected-cattle-farms
#12
Khatereh Alizadeh, Nader Mosavari, Razieh Nazari
OBJECTIVE/BACKGROUND: Mycobacterium tuberculosis, the etiologic agent of tuberculosis, causes large-scale morbidity and mortality, particularly in developing countries. In recent years, there has been a significant increase in the drug-resistant ability of M. tuberculosis, triggering a major public health crisis. A detailed analysis of the evolution of the mycobacterial genome helps to better understand the genotype-phenotype relationship in this bacterium. Different strain typing methods have already revealed the worldwide diversity of mycobacterial isolates...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28043536/assessing-genetic-diversity-of-mycobacterium-tuberculosis-by-spoligotyping-and-is6110-based-restriction-fragment-length-polymorphism-in-north-india
#13
Jitendra Prasad Mathuria, Pragya Sharma, Pradyot Prakash, Jai Kumar Samaria, Vishwa Mohan Katoch, Shampa Anupurba
OBJECTIVE/BACKGROUND: Molecular epidemiology methods are very useful for differentiating between strains, assessing their diversity, and measuring the prevalence of the most circulating strain in an area. Various molecular typing methods using different molecular markers have been utilized worldwide, such as restriction fragment length polymorphism (RFLP), spoligotyping, Mycobacterial Interspersed Repetitive Unit - Variable Number of Tandem Repeat (MIRU-VNTR), and Double repetitive element-PCR (DRE-PCR) typing, for simultaneous detection and epidemiologic typing of Mycobacterium tuberculosis...
December 2016: International Journal of Mycobacteriology
https://www.readbyqxmd.com/read/28036308/early-detection-and-control-of-an-acinetobacter-baumannii-multi-resistant-outbreak-in-a-hospital-in-quito-ecuador
#14
Monica Cartelle Gestal, Jeannete Zurita, Gabriela Gualpa, Cecibel Gonzalez, Ariane Paz Y Mino
INTRODUCTION: Acinetobacter baumannii (ABA) is an important opportunistic pathogen associated with high mortality rates in intensive care units (ICUs). An outbreak in the ICU of a secondary-level hospital in Quito, Ecuador, occurred during April and May 2015 and was successfully controlled. METHODOLOGY: Enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC-PCR) and repetitive element palindromic (REP)-PCR was conducted on all isolates recovered from patients, as well as environmental samples, to confirm the presence of an outbreak...
December 30, 2016: Journal of Infection in Developing Countries
https://www.readbyqxmd.com/read/28034830/pros-and-cons-of-direct-genotyping-on-tuberculosis-clinical-samples
#15
Hamidreza Sadegh, Mansour Kargarpour Kamakoli, Ghazaleh Farmanfarmaei, Morteza Masoumi, Farid Abdolrahimi, Abolfazl Fateh, Nayereh Ebrahimzadeh, Fatemeh Rahimi Jamnani, Farzam Vaziri, Seyed Davar Siadat
OBJECTIVE: Prompt genotyping of Mycobacterium tuberculosis (M. tuberculosis) is crucial for improving molecular epidemiological investigation of tuberculosis (TB). METHODS: We performed a retrospective study to evaluate the use of 24 loci MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number of tandem-repeat) directly on 135 clinical samples from 84 TB patients. RESULTS: There was a direct correlation between genotyping on clinical samples by MIRU-VNTR and bacterial load (P = 0...
December 26, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28033112/three-dimensional-ultrasound-strain-imaging-of-skeletal-muscles
#16
K Gijsbertse, A M J Sprengers, M M Nillesen, H H G Hansen, R G P Lopata, N Verdonschot, C L de Korte
In this study, a multi-dimensional strain estimation method is presented to assess local relative deformation in three orthogonal directions in 3D space of skeletal muscles during voluntary contractions. A rigid translation and compressive deformation of a block phantom, that mimics muscle contraction, is used as experimental validation of the 3D technique and to compare its performance with respect to a 2D based technique. Axial, lateral and (in case of 3D) elevational displacements are estimated using a cross-correlation based displacement estimation algorithm...
January 21, 2017: Physics in Medicine and Biology
https://www.readbyqxmd.com/read/28017947/-the-effects-of-jumping-exercise-on-bones
#17
Yoshihisa Umemura
Strain rate and strain magnitude are important factors when considering osteogenic response to the bone strain imposed by mechanical stress. Jumping is a high-impact exercise that includes these factors. Ten to twenty jumping repetitions per day can increase bone mass and strength. However, the returns diminish with more than ten jumping repetitions because mechanosensitivity declines. Jumping exercise increases bone strength and bone diameters in particular. Although jump training is not suitable for older people, jump training could be an effective tool to prevent osteopenia in younger age groups...
2017: Clinical Calcium
https://www.readbyqxmd.com/read/28011349/elevated-prevalence-of-multidrug-resistant-acinetobacter-baumannii-with-extensive-genetic-diversity-in-the-largest-burn-centre-of-northeast-iran
#18
Nazanin Sarhaddi, Saman Soleimanpour, Hadi Farsiani, Arman Mosavat, Samaneh Dolatabadi, Himen Salimizand, Saeid Amel Jamehdar
OBJECTIVES: The emergence and spread of multidrug-resistant (MDR) Acinetobacter baumannii isolates is now frequently associated with nosocomial infections. The aim of this study was to evaluate the genetic relatedness and patterns of antimicrobial resistance amongst A. baumannii isolated from a burn centre at a teaching hospital in Iran. METHODS: A total of 54 A. baumannii isolates were collected from burn wound infections of hospitalised patients. Antimicrobial susceptibility of the isolates was determined, and genotyping analysis was performed by repetitive extragenic palindromic PCR (rep-PCR)...
December 21, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28009107/detection-of-salmonella-enterica-serovar-montevideo-and-newport-in-free-ranging-sea-turtles-and-beach-sand-in-the-caribbean-and-persistence-in-sand-and-seawater-microcosms
#19
A-K Ives, E Antaki, K Stewart, S Francis, M T Jay-Russell, F Sithole, M T Kearney, M J Griffin, E Soto
Salmonellae are Gram-negative zoonotic bacteria that are frequently part of the normal reptilian gastrointestinal flora. The main objective of this project was to estimate the prevalence of non-typhoidal Salmonella enterica in the nesting and foraging populations of sea turtles on St. Kitts and in sand from known nesting beaches. Results suggest a higher prevalence of Salmonella in nesting leatherback sea turtles compared with foraging green and hawksbill sea turtles. Salmonella was cultured from 2/9 and identified by molecular diagnostic methods in 3/9 leatherback sea turtle samples...
December 23, 2016: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28005945/repetitive-elements-in-mycoplasma-hyopneumoniae-transcriptional-regulation
#20
Amanda Malvessi Cattani, Franciele Maboni Siqueira, Rafael Lucas Muniz Guedes, Irene Silveira Schrank
Transcriptional regulation, a multiple-step process, is still poorly understood in the important pig pathogen Mycoplasma hyopneumoniae. Basic motifs like promoters and terminators have already been described, but no other cis-regulatory elements have been found. DNA repeat sequences have been shown to be an interesting potential source of cis-regulatory elements. In this work, a genome-wide search for tandem and palindromic repetitive elements was performed in the intergenic regions of all coding sequences from M...
2016: PloS One
keyword
keyword
19162
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"