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https://www.readbyqxmd.com/read/28201995/zoonotic-necrotizing-myositis-caused-by-streptococcus-equi-subsp-zooepidemicus-in-a-farmer
#1
Bård Reiakvam Kittang, Veronika Kuchařová Pettersen, Oddvar Oppegaard, Dag Harald Skutlaberg, Håvard Dale, Harald G Wiker, Steinar Skrede
BACKGROUND: Streptococcus equi subsp. zooepidemicus is a beta-hemolytic group C streptococcus mainly causing infections in domesticated animals. Here we describe the first case of zoonotic necrotizing myositis caused by this bacterium. CASE PRESENTATION: The patient was a 73-year-old, previously healthy farmer with two asymptomatic Shetland ponies in his stable. After close contact with the ponies while feeding them, he rapidly developed erythema of his left thigh and sepsis with multiple organ failure...
February 15, 2017: BMC Infectious Diseases
https://www.readbyqxmd.com/read/28195658/risk-of-work-injury-among-adolescent-students-from-single-and-partnered-parent-families
#2
Imelda S Wong, F Curtis Breslin
BACKGROUND: Parental involvement in keeping their children safe at work has been examined in a handful of studies, with mixed results. Evidence has suggested that non-work injury risk is higher among children from single-parent families, but little is known about their risk for work-related injuries. METHODS: Five survey cycles of the Canadian Community Health Survey were pooled to create a nationally representative sample of employed 15-19-year old students (N = 16,620)...
March 2017: American Journal of Industrial Medicine
https://www.readbyqxmd.com/read/28195345/in-vivo-measurements-of-human-neck-skin-elasticity-using-mri-and-finite-element-modeling
#3
Yunqiang An, Changjin Ji, Yong Li, Jianxia Wang, Xinyue Zhang, Yaqi Huang
PURPOSE: The assessment of mechanical properties of the human skin is very important in investigating the mechanism of obstructive sleep apnea, a common disorder characterized by repetitive collapse and obstruction of the upper airway during sleep. In this study, a unique method, combining magnetic resonance imaging (MRI) and finite element modeling (FEM), was developed to obtain the value of the in vivo elastic modulus of the neck skin. MEHTHOD: A total of 22 subjects, 16 males and 6 females, were recruited to participate in the MRI studies...
February 13, 2017: Medical Physics
https://www.readbyqxmd.com/read/28191309/co-existence-of-blaoxa-23-and-blandm-1-genes-of-acinetobacter-baumannii-isolated-from-nepal-antimicrobial-resistance-and-clinical-significance
#4
Prabhu Raj Joshi, Mahesh Acharya, Trishna Kakshapati, Udomluk Leungtongkam, Rapee Thummeepak, Sutthirat Sitthisak
BACKGROUND: Molecular analysis of carbapenem-resistant genes in Acinetobacter baumannii, an emerging pathogen, is less commonly reported from Nepal. In this study we determined the antibiotic susceptibility profile and genetic mechanism of carbapenem resistance in clinical isolates of A. baumannii. METHODS: A. baumannii were isolated from various clinical specimens and identified based on Gram staining, biochemical tests, and PCR amplification of organism specific 16S rRNA and blaOXA-51 genes...
2017: Antimicrobial Resistance and Infection Control
https://www.readbyqxmd.com/read/28182316/tissue-mechanics-during-temporal-summation-of-sequentially-cuff-pressure-induced-pain-in-healthy-volunteers-and-patients-with-painful-osteoarthritis
#5
B Manafi-Khanian, K Kjaer Petersen, L Arendt-Nielsen
BACKGROUND: The phenomenon of temporal summation to repetitive pressure pain stimuli is an important central neural mechanism for pain intensity encoding. This study evaluated the time-dependent behaviour of mechanical characteristics of soft tissue during repeated cuff stimulation used for eliciting temporal summation of cuff pressure-evoked pain. Such information of tissue mechanics is important for the interpretation of the pain response evoked during sequential stimulations. METHODS: Temporal summation was assessed in 16 subjects separated into two groups (healthy controls and severe knee osteoarthritis patients) using a visual analogue scale during 10 repetitive painful cuff stimuli (1-s duration, 1-s break) of the lower leg...
February 9, 2017: European Journal of Pain: EJP
https://www.readbyqxmd.com/read/28168080/the-femoral-neck-mechanoresponse-to-hip-extensors-exercise-a-case-study
#6
Saulo Martelli, Hossein Mokhtarzadeh, Peter Pivonka, Peter R Ebeling
Physical activity is recommended to prevent age-related bone loss. However, the proximal femur mechanoresponse is variable, possibly because of a muscle-dependant mechanoresponse. We compared the proximal femur response with the femoral strain pattern generated by the hip extensor muscles. A healthy participant underwent a six-month unilateral training of the hip extensor muscles using a resistance weight regularly adjusted to the 80% of the one-repetition maximum weight. DXA-based measurements of the areal Bone Mineral Density (aBMD) in the exercise leg were adjusted for changes in the control leg...
2017: Journal of Osteoporosis
https://www.readbyqxmd.com/read/28167525/the-history-of-bordetella-pertussis-genome-evolution-includes-structural-rearrangement
#7
Michael R Weigand, Yanhui Peng, Vladimir Loparev, Dhwani Batra, Katherine E Bowden, Mark Burroughs, Pamela K Cassiday, Jamie K Davis, Taccara Johnson, Phalasy Juieng, Kristen Knipe, Marsenia H Mathis, Andrea M Pruitt, Lori Rowe, Mili Sheth, M Lucia Tondella, Margaret M Williams
: Despite high pertussis vaccine coverage, reported cases of whooping cough (pertussis) have increased over the last decade in the United States and other developed countries. Although Bordetella pertussis is well known for its limited gene sequence variation, recent advances in long-read sequencing technology have begun to reveal genome structural heterogeneity among otherwise indistinguishable isolates, even within geographically or temporally defined epidemics. We have compared rearrangements among complete genome assemblies from 257 B...
February 6, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28166858/genetic-diversity-of-mycobacterium-avium-complex-strains-isolated-in-argentina-by-miru-vntr
#8
B R Imperiale, R D Moyano, A B DI Giulio, M A Romero, M F Alvarado Pinedo, M P Santangelo, G E Travería, N S Morcillo, M I Romano
Mycobacterium avium sp. avium (MAA), M. avium sp. hominissuis (MAH), and M. avium sp. paratuberculosis (MAP) are the main members of the M. avium complex (MAC) causing diseases in several hosts. The aim of this study was to describe the genetic diversity of MAC isolated from different hosts. Twenty-six MAH and 61 MAP isolates were recovered from humans and cattle, respectively. GenoType CM® and IS1311-PCR were used to identify Mycobacterium species. The IS901-PCR was used to differentiate between MAH and MAA, while IS900-PCR was used to identify MAP...
February 7, 2017: Epidemiology and Infection
https://www.readbyqxmd.com/read/28166854/an-outbreak-of-burkholderia-cepacia-complex-infections-associated-with-contaminated-liquid-docusate
#9
Lucila Marquez, Katie N Jones, Elaine M Whaley, Tjin H Koy, Paula A Revell, Ruston S Taylor, M Brooke Bernhardt, Jeffrey L Wagner, James J Dunn, John J LiPuma, Judith R Campbell
OBJECTIVE To investigate an outbreak of Burkholderia cepacia complex and describe the measures that revealed the source. SETTING A 629-bed, tertiary-care, pediatric hospital in Houston, Texas. PATIENTS Pediatric patients without cystic fibrosis (CF) hospitalized in the pediatric and cardiovascular intensive care units. METHODS We investigated an outbreak of B. cepacia complex from February through July 2016. Isolates were evaluated for molecular relatedness with repetitive extragenic palindromic polymerase chain reaction (rep-PCR); specific species identification and genotyping were performed at an independent laboratory...
February 7, 2017: Infection Control and Hospital Epidemiology
https://www.readbyqxmd.com/read/28150578/resistotyping-phenotyping-and-genotyping-of-new-delhi-metallo-%C3%AE-lactamase-ndm-among-gram-negative-bacilli-from-iranian-patients
#10
Dariush Shokri, Mohammad Rabbani Khorasgani, Seyed Masih Fatemi, Abbas Soleimani-Delfan
PURPOSE: The purpose of this study was investigation of New Delhi metallo-β-lactamase (NDM) production among gram negative bacilli. METHODOLOGY: Antibiogram-Resistotyping and detection of NDM in clinical isolates of Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa and comparative evaluation of the diagnostic performance of three phenotypic methods for NDM detection considering PCR as gold standard were performed. Minimum Inhibitory Concentration (MIC) of antibiotics against NDM positive strains by E-test and clonal relationship analysis using Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) in these strains were determined...
February 1, 2017: Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28149918/multiple-large-clusters-of-tuberculosis-in-london-a-cross-sectional-analysis-of-molecular-and-spatial-data
#11
Catherine M Smith, Helen Maguire, Charlotte Anderson, Neil Macdonald, Andrew C Hayward
Large outbreaks of tuberculosis (TB) represent a particular threat to disease control because they reflect multiple instances of active transmission. The extent to which long chains of transmission contribute to high TB incidence in London is unknown. We aimed to estimate the contribution of large clusters to the burden of TB in London and identify risk factors. We identified TB patients resident in London notified between 2010 and 2014, and used 24-locus mycobacterial interspersed repetitive units-variable number tandem repeat strain typing data to classify cases according to molecular cluster size...
January 2017: ERJ Open Research
https://www.readbyqxmd.com/read/28132109/bridging-with-reduced-overlap-fixation-and-peritoneal-grip-can-prevent-slippage-of-dis-class-a-meshes
#12
F Kallinowski, F Harder, T G Silva, A Mahn, M Vollmer
PURPOSE: Ventral hernia repair can be performed safely using meshes which are primarily stable upon dynamic intermittent straining (DIS) at recommended overlap. In specific clinical situations, e.g., at bony edges, bridging of the hernial orifice with reduced overlap might be necessary. To gain insight into the durability of various applications, two different meshes with the best tissue grip known so far were assessed. METHODS: The model uses dynamic intermittent strain and comprises the repetition of submaximal impacts delivered via a hydraulically driven plastic containment...
January 28, 2017: Hernia: the Journal of Hernias and Abdominal Wall Surgery
https://www.readbyqxmd.com/read/28127718/detection-of-toxoplasma-gondii-in-faeces-of-privately-owned-cats-using-two-pcr-assays-targeting-the-b1-gene-and-the-529-bp-repetitive-element
#13
Fabrizia Veronesi, Azzurra Santoro, Giovanni L Milardi, Manuela Diaferia, Giulia Morganti, David Ranucci, Simona Gabrielli
Toxoplasma gondii infection is a worldwide parasitic zoonosis with a high-health risk for humans. The key epidemiological role played by felids is related to oocyst shedding. The present study compared two amplification protocols for the molecular diagnosis of Toxoplasma infection in owned cats. A total of 78 owned cats referred to an Italian university-teaching hospital and exposed to various T. gondii-associated risk factors were sampled for blood and faeces. Faecal specimens were processed by flotation and tested using 2 copro-PCRs targeting the widely used B1 gene and the 529-bp repetitive element (RE)...
January 27, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28127419/towards-long-read-metagenomics-complete-assembly-of-three-novel-genomes-from-bacteria-dependent-on-a-diazotrophic-cyanobacterium-in-a-freshwater-lake-co-culture
#14
Connor B Driscoll, Timothy G Otten, Nathan M Brown, Theo W Dreher
Here we report three complete bacterial genome assemblies from a PacBio shotgun metagenome of a co-culture from Upper Klamath Lake, OR. Genome annotations and culture conditions indicate these bacteria are dependent on carbon and nitrogen fixation from the cyanobacterium Aphanizomenon flos-aquae, whose genome was assembled to draft-quality. Due to their taxonomic novelty relative to previously sequenced bacteria, we have temporarily designated these bacteria as incertae sedis Hyphomonadaceae strain UKL13-1 (3,501,508 bp and 56...
2017: Standards in Genomic Sciences
https://www.readbyqxmd.com/read/28120821/-au-ag-catalysed-expedient-synthesis-of-branched-heneicosafuranosyl-arabinogalactan-motif-of-mycobacterium-tuberculosis-cell-wall
#15
Shivaji A Thadke, Bijoyananda Mishra, Maidul Islam, Sandip Pasari, Sujit Manmode, Boddu Venkateswara Rao, Mahesh Neralkar, Ganesh P Shinde, Gulab Walke, Srinivas Hotha
Emergence of multidrug-resistant and extreme-drug-resistant strains of Mycobacterium tuberculosis (MTb) can cause serious socioeconomic burdens. Arabinogalactan present on the cellular envelope of MTb is unique and is required for its survival; access to arabinogalactan is essential for understanding the biosynthetic machinery that assembles it. Isolation from Nature is a herculean task and, as a result, chemical synthesis is the most sought after technique. Here we report a convergent synthesis of branched heneicosafuranosyl arabinogalactan (HAG) of MTb...
January 25, 2017: Nature Communications
https://www.readbyqxmd.com/read/28119789/integration-of-osteopathic-manual-treatments-in-management-of-cervical-dystonia-with-tremor-a-case-series
#16
Miriam Halimi, Adena Leder, Jayme D Mancini
BACKGROUND: Cervical dystonia, also known as spasmodic torticollis, is a chronic disorder in which patients exhibit involuntary repetitive contractions of neck muscles resulting in abnormal postures or movements. Occasionally, there is also a dystonic head tremor. The underlying mechanisms for cervical dystonia and dystonic tremor are not clear, and treatments are limited. CASE REPORT: In the present cases, two females with head tremor starting in adolescence developed worsening symptoms of cervical dystonia with dystonic tremor in their 60s...
2017: Tremor and Other Hyperkinetic Movements
https://www.readbyqxmd.com/read/28118979/fatigue-injury-risk-in-anterior-cruciate-ligament-of-target-side-knee-during-golf-swing
#17
Tserenchimed Purevsuren, Moon Seok Kwon, Won Man Park, Kyungsoo Kim, Seung Ho Jang, Young-Tae Lim, Yoon Hyuk Kim
A golf-related ACL injury can be linked with excessive golf play or practice because such over-use by repetitive golf swing motions can increase damage accumulation to the ACL bundles. In this study, joint angular rotations, forces, and moments, as well as the forces and strains on the ACL of the target-side knee joint, were investigated for ten professional golfers using the multi-body lower extremity model. The fatigue life of the ACL was also predicted by assuming the estimated ACL force as a cyclic load...
December 14, 2016: Journal of Biomechanics
https://www.readbyqxmd.com/read/28118574/rapd-and-eric-based-typing-of-clinical-and-environmental-pseudomonas-aeruginosa-isolates
#18
Ibtesam Ghadban Auda, Israa M S Al-Kadmy, Sawsan Mohammed Kareem, Aliaa Khyuon Lafta, Mustafa Hussein Obeid A'Affus, Ibrahim Abd Aloahd Khit, Abdulaziz Abdullah Al Kheraif, Darshan Devang Divakar, Ravikumar Ramakrishnaiah
<em>Pseudomonas aeruginosa</em> is a major cause of nosocomial infection in children and adults, resultingin significant morbidity and mortality due to its ability to acquire drug resistance. The ability of <em>P. aeruginosa</em> in the environment to cause infection in individuals has been reported previously; henceforth, surveillance of the emergence and transmission of <em>P. aeruginosa</em> strains among patients is important for infection control in a clinical setup...
November 30, 2016: Journal of AOAC International
https://www.readbyqxmd.com/read/28115905/genetic-diversity-and-symbiotic-efficiency-of-indigenous-common-bean-rhizobia-in-croatia
#19
Ines Pohajda, Katarina Huić Babić, Ivana Rajnović, Sanja Kajić, Sanja Sikora
Nodule bacteria (rhizobia) in symbiotic associations with legumes enable considerable entries of biologically fixed nitrogen into soil. Efforts are therefore made to intensify the natural process of symbiotic nitrogen fixation by legume inoculation. Studies of field populations of rhizobia open up the possibility to preserve and probably exploit some indigenous strains with hidden symbiotic or ecological potentials. The main aim of the present study is to determine genetic diversity of common bean rhizobia isolated from different field sites in central Croatia and to evaluate their symbiotic efficiency and compatibility with host plants...
December 2016: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/28113578/2d-versus-3d-cross-correlation-based-radial-and-circumferential-strain-estimation-using-multiplane-2d-ultrafast-ultrasound-in-a-3d-atherosclerotic-carotid-artery-model
#20
Stein Fekkes, Abigail E S Swillens, Hendrik H G Hansen, Anne E C M Saris, Maartje M Nillesen, Francesco Iannaccone, Patrick Segers, Chris L de Korte
Three-dimensional strain estimation might improve the detection and localization of high strain regions in the carotid artery for identification of vulnerable plaques. This study compares 2D vs. 3D displacement estimation in terms of radial and circumferential strain using simulated ultrasound images of a patient specific 3D atherosclerotic carotid artery model at the bifurcation embedded in surrounding tissue generated with ABAQUS software. Global longitudinal motion was superimposed to the model based on literature data...
August 25, 2016: IEEE Transactions on Ultrasonics, Ferroelectrics, and Frequency Control
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