keyword
MENU ▼
Read by QxMD icon Read
search

Repetitive strain

keyword
https://www.readbyqxmd.com/read/28813779/dna-profiling-of-leuconostoc-citreum-strains-in-fermented-foods-by-repetitive-element-polymerase-chain-reaction
#1
Jasmine Kaur, Anshul Sharma, Sulhee Lee, Young-Seo Park
To identify and discriminate the bacterial species at the sub-specific level, rep-PCR method is a reliable genomic fingerprinting tool. Fourteen strains of bacteria were isolated from different food sources, identified as Leuconostoc citreum using 16S rRNA gene sequencing and amplified using rep-primers (REP, ERIC, and (GTG)5). Fingerprinting patterns generated band range in between 300-6000 bp with REP, 150-6000bp with ERIC and 200-1700 bp with (GTG)5 primers respectively. In UPGMA dendrogram analysis, 14 strains were clustered into three clades (I, II and III) with all the primers, thus differentiating them at the molecular level...
August 17, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28810996/rhabdomyolysis-after-crawling-military-training
#2
Danit Atias-Varon, Haggai Sherman, Ran Yanovich, Yuval Heled
INTRODUCTION: Rhabdomyolysis is a syndrome characterized by muscle necrosis followed by release of intracellular muscle contents into the circulation. Exertional rhabdomyolysis (ER) occurs in response to nonfamiliar and/or excessive, prolonged, or repetitive exercises, with eccentric characteristics. In military populations, due to the type of intense, all out physical loads, ER is a significant threat, particularly when training under heat stress. However, many other etiologies exist, and clinical presentations vary greatly...
July 2017: Military Medicine
https://www.readbyqxmd.com/read/28810248/antibiotic-susceptibility-and-molecular-characterization-of-escherichia-coli-o157-isolates-from-urinary-tract-infections
#3
Zonglan Xie, Ziqiang Dong, Ping Zhu, Lusheng Zhang, Xiaobo Chen, Chuanjiang Dong
BACKGROUND: Escherichia coli O157 is an important food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and hemolytic uraemic syndrome. Enterobacterial repetitive intergenic consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST) are good methods for molecular typing and the extensive use of antibiotics is a contributing factor to the increasing incidence of antimicrobial-resistant for these strains. OBJECTIVES: The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of E...
August 16, 2017: Urologia Internationalis
https://www.readbyqxmd.com/read/28805537/high-prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae-in-wild-fish-from-the-mediterranean-sea-in-algeria
#4
Soumia Brahmi, Abdelaziz Touati, Catherine Dunyach-Remy, Albert Sotto, Alix Pantel, Jean-Philippe Lavigne
AIM: We investigated the prevalence of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae among wild fish from the coast of Bejaia (Algeria) in the Mediterranean Sea. RESULTS: From March 2012 to August 2013, gut and gill samples of wild fish were screened for the presence of ESBL-producing Enterobacteriaceae. Strains were characterized with regard to antibiotic resistance, β-lactamase content, plasmid-mediated quinolone resistance, aminoglycoside resistance genes, and clonality (repetitive sequence-based polymerase chain reaction profiles and multilocus sequence typing)...
August 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28802938/impulse-control-and-related-disorders-in-parkinson-s-disease
#5
Daniel Weintraub, Daniel O Claassen
Impulse control disorders (ICDs), such as compulsive gambling, buying, sexual, and eating behaviors, are a serious and increasingly recognized complication in Parkinson's disease (PD), occurring in up to 20% of PD patients over the course of their illness. Related behaviors include punding (stereotyped, repetitive, purposeless behaviors), dopamine dysregulation syndrome (DDS) (compulsive medication overuse), and hobbyism (e.g., compulsive internet use, artistic endeavors, and writing). These disorders have a significant impact on quality of life and function, strain interpersonal relationships, and worsen caregiver burden, and are associated with significant psychiatric comorbidity...
2017: International Review of Neurobiology
https://www.readbyqxmd.com/read/28802410/evaluation-of-24-locus-miru-vntr-genotyping-in-mycobacterium-tuberculosis-cluster-investigations-in-four-jurisdictions-in-the-united-states-2006-2010
#6
Larry D Teeter, J Steven Kammerer, Smita Ghosh, Duc T M Nguyen, Padmaja Vempaty, Jane Tapia, Roque Miramontes, Wendy A Cronin, Edward A Graviss
The U.S. Centers for Disease Control and Prevention (CDC) uses a combination of spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive units-variable number of tandem repeats (MIRU-VNTR) analyses as part of the National TB Genotyping Service (NTGS). The NTGS expansion from 12-locus MIRU-VNTR (MIRU12) to 24-locus MIRU-VNTR (MIRU24) in 2009 enhanced the ability to discriminate Mycobacterium tuberculosis strains. In the current study, we investigated the MIRU24 concordance among epidemiologic-linked tuberculosis (TB) patients in four U...
September 2017: Tuberculosis
https://www.readbyqxmd.com/read/28800594/species-identification-and-molecular-typing-of-human-brucella-isolates-from-kuwait
#7
Abu S Mustafa, Nazima Habibi, Amr Osman, Faraz Shaheed, Mohd W Khan
Brucellosis is a zoonotic disease of major concern in Kuwait and the Middle East. Human brucellosis can be caused by several Brucella species with varying degree of pathogenesis, and relapses are common after apparently successful therapy. The classical biochemical methods for identification of Brucella are time-consuming, cumbersome, and provide information limited to the species level only. In contrast, molecular methods are rapid and provide differentiation at intra-species level. In this study, four molecular methods [16S rRNA gene sequencing, real-time PCR, enterobacterial repetitive intergenic consensus (ERIC)-PCR and multilocus variable-number tandem-repeat analysis (MLVA)-8, MLVA-11 and MLVA-16 were evaluated for the identification and typing of 75 strains of Brucella isolated in Kuwait...
2017: PloS One
https://www.readbyqxmd.com/read/28781414/test-suite-for-image-based-motion-estimation-of-the-brain-and-tongue
#8
Jordan Ramsey, Jerry L Prince, Arnold D Gomez
Noninvasive analysis of motion has important uses as qualitative markers for organ function and to validate biomechanical computer simulations relative to experimental observations. Tagged MRI is considered the gold standard for noninvasive tissue motion estimation in the heart, and this has inspired multiple studies focusing on other organs, including the brain under mild acceleration and the tongue during speech. As with other motion estimation approaches, using tagged MRI to measure 3D motion includes several preprocessing steps that affect the quality and accuracy of estimation...
February 11, 2017: Proceedings of SPIE
https://www.readbyqxmd.com/read/28776043/amino-acid-differences-in-the-1753-to-1851-region-of-tcdb-influence-variations-in-tcdb1-and-tcdb2-cell-entry
#9
Jonathan J Hunt, Jason L Larabee, Jimmy D Ballard
Clostridium difficile TcdB2 enters cells with a higher efficiency than TcdB1 and exhibits an overall higher level of toxicity. However, the TcdB2-specific sequences that account for more efficient cell entry have not been reported. In this study, we examined the contribution of carboxy-terminal sequence differences to TcdB activity by comparing the binding, uptake, and endosomal localization of TcdB1 and TcdB2 or selected recombinant fragments of these proteins. Our findings suggest that sequence differences in the amino acid 1753 to 1851 region proximal to the combined repetitive oligopeptide domain (CROP) support enhanced uptake of TcdB2 and localization of toxin in acidified endosomes...
July 2017: MSphere
https://www.readbyqxmd.com/read/28766233/prospective-study-comparing-surgeons-pain-and-fatigue-associated-with-nipple-sparing-versus-skin-sparing-mastectomy
#10
Rubie Sue Jackson, Thomas Sanders, Adrian Park, Robert Buras, Wen Liang, Christine Harris, Charles Mylander, Martin Rosman, Luther Holton, Devinder Singh, Laura Martino, Lorraine Tafra
BACKGROUND: Nipple-sparing mastectomy (NSM) is more technically challenging than skin-sparing mastectomy (SSM) but offers quality-of-life and cosmetic advantages. However, surgeon physical symptoms related to NSM workload have not been documented. METHODS: This was a prospective study using questionnaires to compare surgeon-reported physical symptoms before, during, and after NSM versus SSM. Surgeons also answered general questions about each mastectomy. Bilateral cases were performed simultaneously by two surgeons, who completed independent questionnaires...
August 1, 2017: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/28763063/aripiprazole-and-riluzole-treatment-alters-behavior-and-neurometabolites-in-young-adhd-rats-a-longitudinal-1-h-nmr-spectroscopy-study-at-11-7t
#11
F Rizzo, A Abaei, E Nespoli, J M Fegert, B Hengerer, V Rasche, T M Boeckers
Attention deficit hyperactivity disorder (ADHD), Tourette syndrome (TS) as well as obsessive compulsive disorder (OCD) are co-occurring neurodevelopmental diseases that share alterations of frontocortical neurometabolites. In this longitudinal study we investigated the behavioral and neurochemical effects of aripiprazole and riluzole treatment in juvenile spontaneously hypertensive rats (SHR), a model for ADHD. For neurochemical analysis we employed in vivo magnetic resonance spectroscopy (MRS). Spectra from voxels located at the central striatum and prefrontal cortex were acquired postnatally from day 35 to 50...
August 1, 2017: Translational Psychiatry
https://www.readbyqxmd.com/read/28761560/design-and-analysis-of-a-continuous-split-typed-needle-free-injection-system-for-animal-vaccination
#12
Kai Chen, Min Pan, Tingting Liu
BACKGROUND: Liquid needle-free injection devices (NFIDs) employ a high-velocity liquid jet to deliver drugs and vaccine through transdermal injection. NFIDs for animal vaccination are more complicated than those used for human beings for their much larger and more flexible power sources, as well as rapid, repetitive and continuous injection features. METHOD: In the paper, spring-powered NFID is designed for animal vaccine injection. For convenience, the device is a split into a power source and handheld injector...
2017: Open Biomedical Engineering Journal
https://www.readbyqxmd.com/read/28760681/genetic-diversity-of-mycobacterium-tuberculosis-and-transmission-associated-with-first-line-drug-resistance-a-first-analysis-in-jalisco-mexico
#13
Gladys Lopez-Avalos, Guadalupe Gonzalez-Palomar, Martín Lopez-Rodriguez, Carlos Arturo Vazquez-Chacon, Gustavo Mora-Aguilera, Juan Antonio Gonzalez-Barrios, Juan Carlos Villanueva-Arias, Manuel Sandoval-Diaz, Ulises Miranda-Hernández, Ikuri Alvarez-Maya
OBJECTIVES: To analyze the frequency of gene mutations associated to drug resistant in clinical samples from population of the Jalisco state (Mexico) and then evaluate the genetic variability of TB and MDR-TB strains to describe the frequency of M. tuberculosis families. METHODS: We analyzed the clinical isolates of M. tuberculosis obtained from Jalisco state of Mexico. Isolates were subjected to drug susceptibility testing and mutations were characterized by sequencing, followed by genotyping using spoligotyping and Interspersed Repetitive Units-Variable Number of Tandem Repeat (MIRU-VNTR)...
July 28, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28747783/engineering-the-geometrical-shape-of-mesenchymal-stromal-cells-through-defined-cyclic-stretch-regimens
#14
Brandan Walters, Tatiana Uynuk-Ool, Miriam Rothdiener, Julian Palm, Melanie L Hart, Jan P Stegemann, Bernd Rolauffs
Stem cells have been predicted to improve disease outcomes and patient lives. Steering stem cell fate - through controlling cell shape - may substantially accelerate progress towards this goal. As mesenchymal stromal cells (MSCs) are continuously exposed in vivo to a dynamically changing biomechanical environment, we hypothesized that exogenous forces can be applied for engineering a variety of significantly different MSC shapes. We applied specific cyclic stretch regimens to human MSCs and quantitatively measured the resulting cell shape, alignment, and expression of smooth muscle (SMC) differentiation markers, as those have been associated with elongated morphology...
July 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28745309/a-quantitative-and-efficient-approach-to-select-miru-vntr-loci-based-on-accumulation-of-the-percentage-differences-of-strains-for-discriminating-divergent-mycobacterium-tuberculosis-sublineages
#15
Xin-Ling Pan, Chun-Lei Zhang, Chie Nakajima, Jin Fu, Chang-Xia Shao, Li-Na Zhao, Jia-Yi Cui, Na Jiao, Chang-Long Fan, Yasuhiko Suzuki, Toshio Hattori, Di Li, Hong Ling
Although several optimal mycobacterial interspersed repetitive units-variable number tandem repeat (MIRU-VNTR) loci have been suggested for genotyping homogenous Mycobacterium tuberculosis, including the Beijing genotype, a more efficient and convenient selection strategy for identifying optimal VNTR loci is needed. Here 281 M. tuberculosis isolates were analyzed. Beijing genotype and non-Beijing genotypes were identified, as well as Beijing sublineages, according to single nucleotide polymorphisms. A total of 22 MIRU-VNTR loci were used for genotyping...
July 26, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28743648/drug-resistance-and-mycobacterium-tuberculosis-strain-diversity-in-tb-hiv-co-infected-patients-in-ho-chi-minh-city-vietnam
#16
Trinh Quynh Mai, Nguyen Thi Van Anh, Nguyen Tran Hien, Nguyen Huu Lan, Do Chau Giang, Pham Thi Thu Hang, Nguyen Thi Ngoc Lan, Ben J Marais, Vitali Sintchenko
BACKGROUND: Mycobacterium tuberculosis strain diversity and drug resistance among people living with human immunodeficiency virus (HIV) in Vietnam have not been described previously. METHODS: We examined M. tuberculosis isolates from TB/HIV co-infected patients in Ho Chi Minh City, Vietnam. Drug susceptibility testing (DST), spoligotyping and 24-locus Mycobacterial Interspersed Repetitive Unit (MIRU-24 typing) were performed, and the rpoB, katG, inhA and inhA promoter, rpsL, rrs and embB genes were sequenced in all drug resistant isolates identified...
July 22, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28737107/characterization-of-enterococcus-faecalis-isolates-by-chicken-embryo-lethality-assay-and-eric-pcr
#17
Ana E Blanco, Martin Barz, David Cavero, Wiebke Icken, A Reza Sharifi, Matthias Voss, Carlos Buxadé, Rudolf Preisinger
Enterococcus faecalis is the major causative agent of amyloid arthropathy in chickens. Given the difficulty of estimating the risk from field strains, the Embryo Lethality Assay (ELA) is proposed in this study as model to predict the virulence of 68 avian E. faecalis strains. Additionally, Enterobacterial Repetitive Intergenic Consensus Polymerase Chain Reaction (ERIC-PCR) was used to characterize the genetic diversity of the E. faecalis strains. The ELA was performed ten times with subsets of seven-eight E...
July 24, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28729708/new-mycobacterium-tuberculosis-beijing-clonal-complexes-in-china-revealed-by-phylogenetic-and-bayesian-population-structure-analyses-of-24-loci-miru-vntrs
#18
Chao Zheng, Yann Reynaud, Changsong Zhao, Thierry Zozio, Song Li, Dongxia Luo, Qun Sun, Nalin Rastogi
Beijing lineage of Mycobacterium tuberculosis constitutes the most predominant lineage in East Asia. Beijing epidemiology, evolutionary history, genetics are studied in details for years revealing probable origin from China followed by worldwide expansion, partially linked to higher mutation rate, hypervirulence, drug-resistance, and association with cases of mixed infections. Considering huge amount of data available for 24-loci Mycobacterial Interspersed Repetitive Units-Variable Number of Tandem Repeats, we performed detailed phylogenetic and Bayesian population structure analyses of Beijing lineage strains in mainland China and Taiwan using available 24-loci MIRU-VNTR data extracted from publications or the SITVIT2 database (n = 1490)...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28705201/genotypic-diversity-among-multidrug-resistant-pseudomonas-aeruginosa-and-acinetobacter-species-at-mulago-hospital-in-kampala-uganda
#19
David P Kateete, Ritah Nakanjako, Moses Okee, Moses L Joloba, Christine F Najjuka
BACKGROUND: Multidrug resistant Pseudomonas aeruginosa and Acinetobacter species are common causes of nosocomial infections worldwide. Recently we reported the occurrence of carbapenem resistant Enterobacteriaceae, P. aeruginosa and Acinetobacter species at Mulago National Referral Hospital in Kampala, Uganda, but the isolates were not analyzed for genetic relatedness. Herein we report the intra-species genotypic diversity among P. aeruginosa and Acinetobacter baumannii isolated from hospitalized patients and the environment at Mulago Hospital, using repetitive elements-based PCR (Rep-PCR) genotyping...
July 14, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/28696511/direct-application-of-rep-pcr-on-type-i-sourdough-matrix-to-monitor-the-dominance-and-persistence-of-a-lactobacillus-plantarum-starter-throughout-back-slopping
#20
Paola Dolci, Luca Cocolin
This study describes the optimization and application of repetitive element-PCR (rep-PCR) technique directly on microbial DNA extracted from type I sourdoughs for fast monitoring of a Lb. plantarum starter strain (P1FMC) throughout daily back-slopping. The challenge was to follow and study the performance of a starter culture directly in sourdoughs without cultivation on selective media. The extraction of good quality microbial DNA suitable for amplification from a complex matrix such as dough was the first target...
July 11, 2017: Journal of Food Science
keyword
keyword
19162
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"