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https://www.readbyqxmd.com/read/28527285/-genetic-diversity-of-extraintestinal-escherichia-coli-strains-producers-of-beta-lactamases-tem-shv-and-ctx-m-associated-with-healthcare
#1
Yasmin Varela, Beatriz Millán, María Araque
INTRODUCTION: There are few reports from Venezuela describing the genetic basis that sustains the pathogenic potential and phylogenetics of Escherichia coli extraintestinal strains isolated in health care units. OBJECTIVE: To establish the genetic diversity of extraintestinal E. coli strains producers of betalactamases TEM, SHV and CTX-M associated with healthcare. MATERIALS AND METHODS: We studied a collection of 12 strains of extraintestinal E...
June 1, 2017: Biomédica: Revista del Instituto Nacional de Salud
https://www.readbyqxmd.com/read/28514267/systolic-and-diastolic-lv-mechanics-during-and-following-resistance-exercise
#2
Eric J Stöhr, Mike Stembridge, Rob Shave, T Jake Samuel, Keeron Stone, Joseph I Esformes
PURPOSE: To improve the current understanding of the impact of resistance exercise on the heart, by examining the acute responses of left ventricular (LV) strain, twist and untwisting rate ('LV mechanics'). METHODS: LV echocardiographic images were recorded in systole and diastole before, during and immediately after (7-12 s) double leg press exercise at two intensities (30% and 60% of maximum strength, 1-repetition-maximum, 1RM). Speckle tracking analysis generated LV strain, twist and untwisting rate data...
May 16, 2017: Medicine and Science in Sports and Exercise
https://www.readbyqxmd.com/read/28500800/first-insight-into-the-fluoroquinolone-and-aminoglycoside-resistance-of-multidrug-resistant-mycobacterium-tuberculosis-in-saudi-arabia
#3
Bright Varghese, Sahal Al-Hajoj
AbstractIn Saudi Arabia, there were no nationwide screening studies conducted so far to determine the aminoglycoside and fluoroquinolone resistance among multidrug-resistant tuberculosis (MDR-TB) isolates. Therefore, as the first attempt in the country, a retrospective analysis has been conducted on a nationwide collection of 2,956 M. tuberculosis clinical isolates screened with phenotypic drug susceptibility testing to define MDR-TB. Enrolled MDR-TB isolates were subjected to second-line drug susceptibility testing, detection of mutations conferring resistance to aminoglycosides and fluoroquinolone, followed by 24-loci mycobacterial interspersed repetitive unit-variable number of tandem repeat typing and spoligotyping...
May 2017: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/28494342/genome-sequence-variation-among-isolates-of-monkey-b-virus-macacine-alphaherpesvirus-1-from-captive-macaques
#4
R Eberle, L K Maxwell, S Nicholson, D Black, L Jones-Engel
Complete genome sequences of 19 strains of monkey B virus (Macacine alphaherpesvirus 1; BV) isolated from several macaque species were determined. A low level of sequence variation was present among BV isolates from rhesus macaques. Most variation among BV strains isolated from rhesus macaques was located in regions of repetitive or quasi-repetitive sequence. Variation in coding sequences (polypeptides and miRNAs) was minor compared to regions of non-coding sequences. Non-coding sequences in the long and short repeat regions of the genome did however exhibit islands of conserved sequence...
May 7, 2017: Virology
https://www.readbyqxmd.com/read/28482417/-molecular-epidemiologic-study-on-mycobacterium-tuberculosis-from-drug-resistance-monitoring-sites-of-guangdong-province-2015
#5
X C Huang, H X Guo, Z H Wu, C X Guo, W J Wei, H C Li, Q Sun, C C Zhang, Z Y Li, T Chen, Q Zhong, L Zhou
Objective: To understand the characteristics of Mycobacterium tuberculosis (MTB) in epidemiology and distribution from Guangdong Province, and to explore the risk factors associated with drug resistance. Methods: A total of 225 clinical strains of MTB collected from 5 drug resistance monitoring sites of Guangdong Province in 2015 were tested by Regions of Difference 105 (RD105) deletion test and 15 loci mycobacterial interspersed repetitive units (MIRU) were used for genotyping. Gene clustering was analyzed using BioNumerics7...
May 12, 2017: Chinese Journal of Tuberculosis and Respiratory Diseases
https://www.readbyqxmd.com/read/28463273/100-nm-tunable-femtosecond-cr-lisaf-laser-mode-locked-with-a-broadband-saturable-bragg-reflector
#6
Umit Demirbas, Jing Wang, Gale S Petrich, Sheila Nabanja, Jonathan R Birge, Leslie A Kolodziejski, Franz X Kärtner, James G Fujimoto
We report broad tunability of a femtosecond (fs) diode-pumped Cr:LiSAF laser mode-locked with a broadband saturable Bragg reflector (SBR). The SBR had seven pairs of Al<sub>x</sub>O<sub>y</sub>(n∼1.5) and Al<sub>0.17</sub>Ga<sub>0.83</sub>As(n∼3.5) layers in its Bragg stack, enabling a 250 nm reflectivity bandwidth around 850 nm. A 6-nm-thick strained In<sub>0.15</sub>Ga<sub>0.85</sub>As quantum well placed between Al<sub>0...
May 1, 2017: Applied Optics
https://www.readbyqxmd.com/read/28459795/four-weeks-of-nordic-hamstring-exercise-reduce-muscle-injury-risk-factors-in-young-adults
#7
João Breno de A R Alvares, Vanessa Bernardes Marques, Marco Aurélio Vaz, Bruno Manfredini Baroni
The Nordic hamstring exercise (NHE) is a field-based exercise designed for knee-flexor eccentric strengthening, aimed at muscle strains prevention. However, possible effects of NHE programmes on other hamstring injury risk factors remain unclear. The purpose of this study was to investigate the effects of a NHE training programme on multiple hamstring injury risk factors. Twenty physically active young adults were allocated into two equal sized groups: control group (CG) and training group (TG). The TG was engaged in a 4-week NHE programme, twice a week, 3 sets of 6-10 repetitions; while CG received no exercise intervention...
April 26, 2017: Journal of Strength and Conditioning Research
https://www.readbyqxmd.com/read/28456703/antimicrobial-susceptibility-and-mechanisms-of-fosfomycin-resistance-in-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-strains-from-urinary-tract-infections-in-wenzhou-china
#8
Wenzi Bi, Bin Li, Jiangning Song, Youliang Hong, Xiaoxiao Zhang, Haiyang Liu, Hong Lu, Tieli Zhou, Jianming Cao
Fosfomycin in combination with various antibiotics represents an excellent clinically efficacious regimen for the treatment of urinary tract infections (UTIs) caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli. Underlying mechanisms of fosfomycin resistance remain largely uncharacterised. To investigate the antibacterial efficacy of fosfomycin against ESBL-producing E. coli, 356 non-repetitive ESBL-producing E. coli clinical isolates were collected from urine specimens from patients with UTI in Wenzhou, China, from January 2011 to December 2015...
April 26, 2017: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/28454647/genotyping-and-rifampicin-and-isoniazid-resistance-in-mycobacterium-bovis-strains-isolated-from-the-lymph-nodes-of-slaughtered-cattle
#9
Marília Masello Junqueira Franco, Márcio Garcia Ribeiro, Fernando Rogério Pavan, Marcelo Miyata, Marcos Bryan Heinemann, Antonio Francisco de Souza Filho, Rosilene Fressatti Cardoso, Aryadne Larissa de Almeida, Ricardo Ichiro Sakate, Antonio Carlos Paes
In developing nations, 10-20% of the human cases of tuberculosis are caused by Mycobacterium bovis. However, this percentage may be underestimated because most laboratories in developing countries do not routinely perform mycobacterial cultures, and only a few have the systems in place to identify M. bovis. There are few studies investigating genotypic diversity and drug resistance in M. bovis from animal and/or human infections. The genotypic diversity of M. bovis strains obtained from bovine lymph nodes were investigated by spacer oligonucleotide typing (spoligotyping) and mycobacterial interspersed repetitive unit-variable-number tandem repeat typing (MIRU-VNTR)...
May 2017: Tuberculosis
https://www.readbyqxmd.com/read/28452235/molecular-characteristics-biofilm-forming-abilities-and-quorum-sensing-molecules-in-vibrio-parahaemolyticus-strains-isolated-from-marine-and-clinical-environments-in-korea
#10
Md Furkanur Rahaman Mizan, Hyeon-Jo Bang, Mohammad Sadekuzzaman, Nari Lee, Tae-Jo Kim, Sang-Do Ha
Vibrio parahaemolyticus is an inhabitant of marine and estuarine environments and causes seafood-borne gastroenteritis in humans. In this study, an UltraFast LabChip Real-Time PCR assay was evaluated for rapid detection and quantification of pathogenic V. parahaemolyticus isolates. Escherichia coli and Vibrio harveyi were used as negative controls. Twenty-six tdh-positive, biofilm-producing V. parahaemolyticus isolates were analyzed by repetitive extragenic palindromic-polymerase chain reaction (REP-PCR). REP-PCR analysis showed that the majority of the V...
April 28, 2017: Biofouling
https://www.readbyqxmd.com/read/28431487/design-and-fuzzy-logic-control-of-an-active-wrist-orthosis
#11
Ergin Kilic, Erdi Dogan
People who perform excessive wrist movements throughout the day because of their professions have a higher risk of developing lateral and medial epicondylitis. If proper precautions are not taken against these diseases, serious consequences such as job loss and early retirement can occur. In this study, the design and control of an active wrist orthosis that is mobile, powerful and lightweight is presented as a means to avoid the occurrence and/or for the treatment of repetitive strain injuries in an effective manner...
April 1, 2017: Proceedings of the Institution of Mechanical Engineers. Part H, Journal of Engineering in Medicine
https://www.readbyqxmd.com/read/28430147/suitability-of-strain-gage-sensors-for-integration-into-smart-sport-equipment-a-golf-club-example
#12
Anton Umek, Yuan Zhang, Sašo Tomažič, Anton Kos
Wearable devices and smart sport equipment are being increasingly used in amateur and professional sports. Smart sport equipment employs various sensors for detecting its state and actions. The correct choice of the most appropriate sensor(s) is of paramount importance for efficient and successful operation of sport equipment. When integrated into the sport equipment, ideal sensors are unobstructive, and do not change the functionality of the equipment. The article focuses on experiments for identification and selection of sensors that are suitable for the integration into a golf club with the final goal of their use in real time biofeedback applications...
April 21, 2017: Sensors
https://www.readbyqxmd.com/read/28429374/immune-response-of-highly-conserved-influenza-a-virus-matrix-1-peptides
#13
Neha Lohia, Manoj Baranwal
Influenza vaccine development is considered to be complicated and challenging. Constantly evolving influenza viruses require continuous global monitoring and reformulation of the vaccine strains. Peptide conserved among different strains and subtypes of influenza A virus are well-thought-out to be attractive targets for development of cross protective influenza vaccine based on cellular response. Three highly conserved (> 90%) matrix 1 peptides ILGFVFTLTVPSERGLQRRRF (PM 1), LIRHENRMVLASTTAKA (PM 2) and LQAYQKRMGVQMQR (PM 3) containing multiple T cell epitopes have been assessed for their immunogenic potential in vitro, subjecting peripheral blood mononuclear cells from healthy volunteers to repetitive stimulation of these chemically synthesised peptides and measuring their interferon (IFN)-γ level and proliferation by ELISA (Enzyme-linked immunosorbent assay) and MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide) assay, respectively...
April 21, 2017: Microbiology and Immunology
https://www.readbyqxmd.com/read/28421811/characterization-of-ornithobacterium-rhinotracheale-field-isolates-from-hungary
#14
Réka Szabó, Enikő Wehmann, László Makrai, Csaba Nemes, Éva Gyuris, Ákos Thuma, Tibor Magyar
Ornithobacterium rhinotracheale is a widely distributed rod-shaped gram-negative bacterium that infects several avian species including chickens and turkeys. It is associated with respiratory signs, growth retardation, mortality, and reduced egg production thus causing severe economic losses to the poultry industries. In this study, 37 field isolates of O. rhinotracheale, collected from various locations in Hungary between 1997 and 2015, were identified and molecularly characterized by the analysis of partial 16S rRNA gene sequences, enterobacterial repetitive intergenic consensus (ERIC)-PCR and random amplified polymorphic DNA (RAPD) PCR assays with the OPG11, OPH19, and M13 primers...
April 19, 2017: Avian Pathology: Journal of the W.V.P.A
https://www.readbyqxmd.com/read/28419868/ciprofloxacin-resistance-in-uropathogenic-escherichia-coli-isolates-causing-community-acquired-urinary-infections-in-bras%C3%A3-lia-brazil
#15
Rafaella Christina Rocha Moreira da Silva, Paulo de Oliveira Martins Júnior, Laura Fernandes Gonçalves, Vicente de Paulo Martins, Ana Beatriz Fabrício de Melo, André Pitondo-Silva, Tatiana Amabile de Campos
OBJECTIVES: Considering the global concern of ciprofloxacin resistance, the aim of this study was to evaluate the characteristics of ciprofloxacin-resistant (CIP-R) Escherichia coli isolated from patients with community-acquired urinary tract infections (UTIs) in Brasília, Brazil. METHODS: CIP-R E. coli isolated from different outpatients between July 2013 and April 2014 in a tertiary hospital were analysed for antibiotic resistance profile, phylotype, uropathogenic E...
April 15, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28415976/smrt-genome-assembly-corrects-reference-errors-resolving-the-genetic-basis-of-virulence-in-mycobacterium-tuberculosis
#16
Afif Elghraoui, Samuel J Modlin, Faramarz Valafar
BACKGROUND: The genetic basis of virulence in Mycobacterium tuberculosis has been investigated through genome comparisons of virulent (H37Rv) and attenuated (H37Ra) sister strains. Such analysis, however, relies heavily on the accuracy of the sequences. While the H37Rv reference genome has had several corrections to date, that of H37Ra is unmodified since its original publication. RESULTS: Here, we report the assembly and finishing of the H37Ra genome from single-molecule, real-time (SMRT) sequencing...
April 17, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28409126/genomics-and-comparative-genomic-analyses-provide-insight-into-the-taxonomy-and-pathogenic-potential-of-novel-emmonsia-pathogens
#17
COMPARATIVE STUDY
Ying Yang, Qiang Ye, Kang Li, Zongwei Li, Xiaochen Bo, Zhen Li, Yingchun Xu, Shengqi Wang, Peng Wang, Huipeng Chen, Junzhi Wang
Over the last 50 years, newly described species of Emmonsia-like fungi have been implicated globally as sources of systemic human mycosis (emmonsiosis). Their ability to convert into yeast-like cells capable of replication and extra-pulmonary dissemination during the course of infection differentiates them from classical Emmonsia species. Immunocompromised patients are at highest risk of emmonsiosis and exhibit high mortality rates. In order to investigate the molecular basis for pathogenicity of the newly described Emmonsia species, genomic sequencing and comparative genomic analyses of Emmonsia sp...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/28399998/bacterial-ecology-of-artisanal-minas-cheeses-assessed-by-culture-dependent-and-independent-methods
#18
Luana Martins Perin, Maria Luisa Savo Sardaro, Luís Augusto Nero, Erasmo Neviani, Monica Gatti
Artisanal Minas cheese is produced in Minas Gerais state, Brazil and its varieties are named according to their geographical origin (Serro, Canastra, Serra do Salitre, Araxá and Campo das Vertentes). The cheese is produced with raw cow's milk and the whey from the previous cheese production ("pingo"). The high economic and cultural importance of artisanal cheese in Brazil justifies the efforts to ensure its safety, quality and provenance. This study aimed to characterize the microbial diversity composition, and geographical distribution of artisanal Minas cheese, focusing on the characterization of its autochthonous lactic acid bacteria (LAB) microbiota...
August 2017: Food Microbiology
https://www.readbyqxmd.com/read/28391652/investigation-on-the-incidence-of-adverse-reactions-viraemia-and-haematological-changes-following-field-immunization-of-cattle-using-a-live-attenuated-vaccine-against-lumpy-skin-disease
#19
P-D Katsoulos, S C Chaintoutis, C I Dovas, Z S Polizopoulou, G D Brellou, E I Agianniotaki, K E Tasioudi, E Chondrokouki, O Papadopoulos, H Karatzias, C Boscos
The present study was performed to investigate the clinical impact and certain virological and haematological parameters following immunization of cattle against lumpy skin disease (LSD). The study was conducted in a dairy cattle farm (215 animals), immunized with a Neethling strain-based live vaccine. Twenty-seven animals (14 lactating cows, four dry cows and nine calves) were randomly selected for repetitive blood and saliva samplings. An EvaGreen-based real-time PCR was designed to differentiate vaccine from field LSDVs...
April 8, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28385004/molecular-and-genomic-features-of-mycobacterium-bovis-strain-1595-isolated-from-korean-cattle
#20
Narae Kim, Yunho Jang, Jin Kyoung Kim, Soyoon Ryoo, Ka Hee Kwon, Miso Kim, Shin Seok Kang, Hyeon Seop Byeon, Hee Soo Lee, Young-Hee Lim, Jae-Myung Kim
The aim of this study was to investigate the molecular characteristics and conduct a comparative genomic analysis for Mycobacterium bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined using Illumina MiSeq and PacBio single-molecule real-time technology, which showed a genome of 4,351,712 bp in size with a 65...
April 6, 2017: Journal of Veterinary Science
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