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Jonpaul Nevin, Mark Waldron, Stephen Patterson, Paul Smith, Mike Price, Alex Hunt
The aim of the present study was to investigate the effects of an 8-week concurrent strength and endurance training programme in comparison to endurance training only on several key determinants of hand cycling performance. Five H4 and five H3 classified hand cyclists with at least one year's hand cycling training history consented to participate in the study. Subjects underwent a battery of tests to establish body mass, body composition, VO2peak, maximum aerobic power, gross mechanical efficiency, maximal upper body strength, and 30 km time trial performance...
March 20, 2018: Journal of Strength and Conditioning Research
Lu Gan, Yanan Yang, Qian Li, Yi Feng, Tianshu Liu, Weijian Guo
Enhancer of zeste homolog 2 (EZH2), a histone methyltransferase and a catalytic component of PRC2, catalyzes tri-methylation of histone H3 at Lys 27 (H3K27me3) to regulate gene expression through epigenetic machinery. EZH2 also functions both as a transcriptional suppressor and a transcriptional co-activator, depending on H3K27me3 or not and on the different cellular contexts. Unsurprisingly, numerous studies have highlighted the role of EZH2 in cancer development and progression. Through modulating critical gene expression, EZH2 promotes cell survival, proliferation, epithelial to mesenchymal, invasion, and drug resistance of cancer cells...
2018: Biomarker Research
Klara Habartova, Radim Havelek, Martina Seifrtova, Karel Kralovec, Lucie Cahlikova, Jakub Chlebek, Eva Cermakova, Nadezda Mazankova, Jana Marikova, Jiri Kunes, Lucie Novakova, Martina Rezacova
Scoulerine is an isoquinoline alkaloid, which indicated promising suppression of cancer cells growth. However, the mode of action (MOA) remained unclear. Cytotoxic and antiproliferative properties were determined in this study. Scoulerine reduces the mitochondrial dehydrogenases activity of the evaluated leukemic cells with IC50 values ranging from 2.7 to 6.5 µM. The xCELLigence system revealed that scoulerine exerted potent antiproliferative activity in lung, ovarian and breast carcinoma cell lines. Jurkat and MOLT-4 leukemic cells treated with scoulerine were decreased in proliferation and viability...
March 19, 2018: Scientific Reports
Albert Ardevol, Gerhard Hummer
Channelrhodopsin-2 (ChR2) is a light-sensitive ion channel widely used in optogenetics. Photoactivation triggers a trans -to- cis isomerization of a covalently bound retinal. Ensuing conformational changes open a cation-selective channel. We explore the structural dynamics in the early photocycle leading to channel opening by classical (MM) and quantum mechanical (QM) molecular simulations. With QM/MM simulations, we generated a protein-adapted force field for the retinal chromophore, which we validated against absorption spectra...
March 19, 2018: Proceedings of the National Academy of Sciences of the United States of America
Mahendra Shukla, Femi M Francis, Jawahar Lal
Conessine, a steroidal alkaloid obtained from the bark and seeds of the plant species of Apocynaceae family, elicits a histamine antagonistic action, selectively for the H3 histaminergic receptors. This alkaloid is used mainly for the treatment of dysentery and helminthic disorders. For the quantification of conessine in serum, a liquid chromatography-tandem mass spectrometry method was developed. Chromatographic separation was achieved on a Zorbax SB-CN column (100 × 4.6 mm, 3.5 µm), and a mobile phase consisting of 90% methanol in aqueous ammonium acetate buffer (pH 3...
January 1, 2018: European Journal of Mass Spectrometry
Long-Fei Wang, Jiang-Zhen Qiu, Si-Guo Wu, Yan-Cong Chen, Cui-Jin Li, Quan-Wen Li, Jun-Liang Liu, Ming-Liang Tong
A chiral Co(II)-based coordination polymer, [Co3 (pimda)2 (H2 O)5 ] (1, H3 pimda = 2-propyl-1H-imidazole-4,5-dicarboxylic acid) with 3D hyperkagomé topology is reported. Upon heating/cooling, the water molecules which are coordinated to a pair of crystallographically symmetric Co(II) ions are removed/recovered discretely in two steps, giving [Co3 (pimda)2 (H2 O)4 ] (2) and [Co3 (pimda)2 (H2 O)3 ] (3), which is evidenced by the reversible single-crystal-to-single-crystal (SCSC) structural transformations. As the coordination geometry of the two Co(II) ions changes from octahedron to trigonal bipyramid, obvious color change from pink for 1 to dark violet for 2 and 3 is observed...
March 19, 2018: Inorganic Chemistry
Meng-Ting Li, Xi-Ya Yang, Ji-Sen Li, Ning Sheng, Guo-Dong Liu, Jing-Quan Sha, Ya-Qian Lan
For exploring the multifold helical fabrication of polyoxometalate (POM)-based hybrid compounds, four POM-based crystalline compounds with different meso-helices, H3 [Ag27 (trz)16 (H2 O)6 ][SiW12 O40 ]2 ·5H2 O (1), H[Ag27 (trz)16 (H2 O)4 ][PW12 O40 ]2 ·2H2 O (2), [Ag23 (trz)14 (H2 O)2 ][HSiW12 O40 ] (3), and [Ag23 (trz)14 (H2 O)2 ][PW12 O40 ] (4), were successfully isolated by using the delicate 1,2,3-triazole ligand and silver ions in this work. Crystal analysis reveals that compounds 1 and 2 and compounds 3 and 4 are isomorphous and display 2-/4-fold mixed meso-helices and simple 2-fold meso-helices, respectively...
March 19, 2018: Inorganic Chemistry
Pinar Cevik, Oguz Eraslan, Kursat Eser, Suleyman Tekeli
PURPOSE: The aim of this study was to evaluate the effect of six different surface conditioning methods on the shear bond strength of ceramic brackets bonded to feldspathic porcelain. MATERIALS AND METHODS: A total of 60 feldspathic porcelain disks were fabricated and divided into six subgroups including 10 specimens in each. Specimens were first treated one of the following surface conditioning methods, namely, 37% phosphoric acid (G-H3PO4), 9.4% hydrofluoric acid (G-HF), grinding with diamond burs (G-Grinding), Nd:YAG laser (G-Nd:YAG), Airborne-particle abrasion (G-Abrasion)...
March 2018: International Journal of Artificial Organs
Yuan Luo, Teena Mohan, Wandi Zhu, Chao Wang, Lei Deng, Bao-Zhong Wang
Seasonal influenza vaccines have proven to be effective against well-matched viruses in healthy adults. However, rapid accumulation of mutations in the main antigenic surface proteins of influenza can compromise the efficiency of flu vaccines. Occasionally, influenza pandemics arise and present a different type of challenge to current seasonal vaccines. Novel vaccination strategies that can educate the host immune system to generate immune responses focusing on conserved epitopes on theses antigenic surface proteins are crucial for controlling and limiting influenza epidemics and pandemics...
March 15, 2018: Scientific Reports
Mariola A Dietrich, Piotr Hliwa, Mikołaj Adamek, Dieter Steinhagen, Halina Karol, Andrzej Ciereszko
The environmental temperature affects plasma biochemical indicators, antioxidant status and hematological and immunological parameters in fish. So far, only single blood proteins have been identified in response to temperature changes. The aim of this study was to compare the proteome of carp blood plasma from males acclimated to warm (30 °C) and cold (10 °C) temperatures by two-dimensional differential gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. A total of 47 spots were found to be differentially regulated by temperature (>1...
March 12, 2018: Fish & Shellfish Immunology
Daniela Munro-Rojas, Esdras Fernandez-Morales, José Zarrabal-Meza, Ma Teresa Martínez-Cazares, Aurora Parissi-Crivelli, Javier Fuentes-Domínguez, Marie Nancy Séraphin, Michael Lauzardo, Jorge Alberto González-Y-Merchand, Sandra Rivera-Gutierrez, Roberto Zenteno-Cuevas
BACKGROUND: Mexico is one of the most important contributors of drug and multidrug-resistant tuberculosis in Latin America; however, knowledge of the genetic diversity of drug-resistant tuberculosis isolates is limited. METHODS: In this study, the genetic structure of 112 Mycobacterium tuberculosis strains from the southeastern Mexico was determined by spoligotyping and 24-loci MIRU-VNTRs. FINDINGS: The results show eight major lineages, the most of which was T1 (24%), followed by LAM (16%) and H (15%)...
2018: PloS One
Miguel A Esteruelas, Ana M López, Enrique Oñate, Ainhoa San-Torcuato, Jui-Yi Tsai, Chuanjun Xia
The preparation and photophysical properties of heteroleptic iridium(III) complexes containing a dianionic C,C,C,C-tetradentate ligand and a cyclometalated phenylpyridine group are described. Complex [Ir(μ-OMe)(COD)]2 (1, COD = 1,5-cyclooctadiene) reacts with 1,1-diphenyl-3,3-butylenediimidazolium iodide ([PhIm(CH2 )4 ImPh]I2 ), in the presence NaOt Bu, to give [Ir(μ-I){κ4 - C, C, C, C-[C6 H4 Im(CH2 )4 ImC6 H4 ]}]2 (2), which leads to {[Ir{κ4 - C, C, C, C-[C6 H4 Im(CH2 )4 ImC6 H4 ]}]2 (μ-OH)(μ-OMe)} (3) by treatment first with silver trifluoromethanesulfonate (AgOTf) in acetone-dichloromethane and subsequently with KOH in methanol...
March 14, 2018: Inorganic Chemistry
J A Peñafiel-Castro, B Hahn, R L Maltez, G Knörnschild, P Allongue, L F P Dick
In this work, compact titanium oxide layers are formed by anodization in 1 M H3 PO4 aqueous electrolyte containing LiClO4 . Elastic Recoil Detection Analysis (ERDA) measurements demonstrate that incorporation of lithium ions occurs during anodization, and cell-voltage transient measurements evidence the different electronic properties of Li-doped TiO2 layers. The mechanism of direct incorporation of Li during the fabrication of TiO2 layers is discussed.
March 14, 2018: Chemical Communications: Chem Comm
Aaron M Thomas, Michael Lucas, Long Zhao, Jerid Liddiard, Ralf I Kaiser, Alexander M Mebel
The crossed molecular beams technique was utilized to explore the formation of three isomers of resonantly stabilized (C5 H3 ) radicals along with their d2 -substituted counterparts via the bimolecular reactions of singlet/triplet dicarbon [C2 (X1 Σ/a3 Πu )] with methylacetylene [CH3 CCH(X1 A1 )], d3 -methylacetylene [CD3 CCH(X1 A1 )], and 1-butyne [C2 H5 CCH(X1 A')] at collision energies up to 26 kJ mol-1 via chemically activated singlet/triplet C5 H4 /C5 D3 H and C6 H6 intermediates. These studies exploit a newly developed supersonic dicarbon [C2 (X1 Σ/a3 Πu )] beam generated via photolysis of tetrachloroethylene [C2 Cl4 (X1 Ag )] by excluding interference from carbon atoms, which represent the dominating (interfering) species in ablation-based dicarbon sources...
March 14, 2018: Physical Chemistry Chemical Physics: PCCP
Lisa B Caruso, Kayla A Martin, Elisabetta Lauretti, Michael Hulse, Micheal Siciliano, Lena N Lupey-Green, Aaron Abraham, Tomasz Skorski, Italo Tempera
The enzyme Poly(ADP-ribose) polymerase 1 (PARP1) plays a very important role in the DNA damage response, but its role in numerous aspects is not fully understood. We recently showed that in the absence of DNA damage, PARP1 regulates the expression of the chromatin-modifying enzyme EZH2. Work from other groups has shown that EZH2 participates in the DNA damage response. These combined data suggest that EZH2 could be a target of PARP1 in both untreated and genotoxic agent-treated conditions. In this work we tested the hypothesis that, in response to DNA damage, PARP1 regulates EZH2 activity...
February 13, 2018: Oncotarget
Yuanyuan Ma, Haiyue Peng, Jianing Liu, Yonghui Wang, Xiuli Hao, Xiaojia Feng, Shifa Ullah Khan, Huaqiao Tan, Yangguang Li
Polyoxometalates (POMs) show considerable catalytic performance toward the selective oxidation of alkenes to aldehydes, which is commercially valuable for the production of pharmaceuticals, dyes, perfumes, and fine chemicals. However, the low specific surface area of POMs as heterogeneous catalysts and poor recyclability as homogeneous catalysts have hindered their wide application. Dispersing POMs into metal-organic frameworks (MOFs) for the construction of POM-based MOFs (POMOFs) suggests a promising strategy to realize the homogeneity of heterogeneous catalysis...
March 13, 2018: Inorganic Chemistry
Mitsunori Shiroishi, Yuji Ito, Kenta Shimokawa, Jae Man Lee, Takahiro Kusakabe, Tadashi Ueda
Rheumatoid factors (RFs) are autoantibodies against the fragment-crystallizable (Fc) region of immunoglobulin G (IgG). In individuals with hematological diseases such as cryoglobulinemia and certain B-cell lymphoma forms, the RFs derived from specific heavy- and light-chain germline pairs, so-called "stereotypic RFs," are frequently produced in copious amounts and form immune complexes with IgG in serum. Of note, many structural details of the antigen-recognition mechanisms in RFs are unclear. Here, we report the crystal structure of the RF YES8c derived from the IGHV1-69/IGKV3-20 germline pair, the commonest of the stereotypic RFs, in complex with human IgG1-Fc at 2...
March 9, 2018: Journal of Biological Chemistry
Marc-Werner Dobenecker, Joon Seok Park, Jonas Marcello, Michael T McCabe, Richard Gregory, Steven D Knight, Inmaculada Rioja, Anna K Bassil, Rabinder K Prinjha, Alexander Tarakhovsky
Differentiation and activation of T cells require the activity of numerous histone lysine methyltransferases (HMT) that control the transcriptional T cell output. One of the most potent regulators of T cell differentiation is the HMT Ezh2. Ezh2 is a key enzymatic component of polycomb repressive complex 2 (PRC2), which silences gene expression by histone H3 di/tri-methylation at lysine 27. Surprisingly, in many cell types, including T cells, Ezh2 is localized in both the nucleus and the cytosol. Here we show the presence of a nuclear-like PRC2 complex in T cell cytosol and demonstrate a role of cytosolic PRC2 in T cell antigen receptor (TCR)-mediated signaling...
March 9, 2018: Journal of Experimental Medicine
Yutaka Kofuku, Tomoki Yokomizo, Shunsuke Imai, Yutaro Shiraishi, Mei Natsume, Hiroaki Itoh, Masayuki Inoue, Kunio Nakata, Shunsuke Igarashi, Hideyuki Yamaguchi, Toshimi Mizukoshi, Ei-Ichiro Suzuki, Takumi Ueda, Ichio Shimada
G protein-coupled receptors (GPCRs) exist in equilibrium between multiple conformations, and their populations and exchange rates determine their functions. However, analyses of the conformational dynamics of GPCRs in lipid bilayers are still challenging, because methods for observations of NMR signals of large proteins expressed in a baculovirus-insect cell expression system (BVES) are limited. Here, we report a method to incorporate methyl-13 C1 H3 -labeled alanine with > 45% efficiency in highly deuterated proteins expressed in BVES...
March 8, 2018: Journal of Biomolecular NMR
Hyungwoo Cho, Dok Hyun Yoon, Kyu-Pyo Kim, Kyun-Seop Bae, Won Seog Kim, Hyeon-Seok Eom, Jin Seok Kim, Jung Yong Hong, Seok Jin Kim, Hyewon Lee, Soo-Jeong Kim, Cheolwon Suh
Background The objective of this study was to assess the safety, dose-limiting toxicities (DLTs), maximum tolerated dose (MTD), pharmacokinetics, and anti-tumor efficacy of CKD-581, a novel pan-histone deacetylase inhibitor, in patients with lymphoma or multiple myeloma (MM) refractory to standard therapy. Methods In this phase I study, CKD-581 was intravenously administered on days 1, 8, and 15 of a 28-day cycle. A standard 3 + 3 cohort design was used to determine the MTD. Acetylated histones H3 and H4 in peripheral blood mononuclear cells were measured for pharmacodynamic assessment in a subpopulation of patients...
March 9, 2018: Investigational New Drugs
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