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https://www.readbyqxmd.com/read/27914462/recombinant-mhc-tetramers-for-isolation-of-virus-specific-cd8-cells-from-healthy-donors-potential-approach-for-cell-therapy-of-posttransplant-cytomegalovirus-infection
#1
A S Vdovin, S Y Filkin, P R Yefimova, S A Sheetikov, N M Kapranov, Y O Davydova, E S Egorov, E G Khamaganova, M Y Drokov, L A Kuzmina, E N Parovichnikova, G A Efimov, V G Savchenko
Patients undergoing allogeneic hematopoietic stem cell transplantation have a high risk of cytomegalovirus reactivation, which in the absence of T-cell immunity can result in the development of an acute inflammatory reaction and damage of internal organs. Transfusion of the virus-specific donor T-lymphocytes represents an alternative to a highly toxic and often ineffective antiviral therapy. Potentially promising cell therapy approach comprises transfusion of cytotoxic T-lymphocytes, specific to the viral antigens, immediately after their isolation from the donor's blood circulation without any in vitro expansion...
November 2016: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/27910250/a-large-%C3%AF-extended-carbon-nanoring-based-on-nanographene-units-bottom-up-synthesis-photophysical-properties-and-selective-complexation-with-fullerene-c70
#2
Dapeng Lu, Guilin Zhuang, Haotian Wu, Song Wang, Shangfeng Yang, Pingwu Du
Herein we report the organoplatinum-mediated bottom-up synthesis, characterization, and properties of a novel large π-extended carbon nanoring based on a nanographene hexa-peri-hexabenzocoronene (HBC) building unit. This tubular structure can be considered as an example of the longitudinal extension of the cycloparaphenylene scaffold to form a large π-extended carbon nanotube (CNT) segment. The cyclic tetramer of a tetramesityl HBC ([4]CHBC) was synthesized by the reaction of a 2,11-diborylated hexa-peri-hexabenzocoronene with a platinum complex, followed by reductive elimination...
December 2, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27906052/antibody-responses-to-de-novo-identified-citrullinated-fibrinogen-peptides-in-rheumatoid-arthritis-and-visualization-of-the-corresponding-b-cells
#3
Vijay Joshua, Loes Schobers, Philip J Titcombe, Lena Israelsson, Johan Rönnelid, Monika Hansson, Anca I Catrina, Ger J M Pruijn, Vivianne Malmström
BACKGROUND: Antibodies against citrullinated proteins (ACPA) are common in patients with rheumatoid arthritis (RA). ACPA can appear before disease onset and target many self-antigens. Citrullinated fibrin/fibrinogen represents a classical ACPA target antigen, and mass spectrometry of RA synovial fluid reveals elevated citrullinated (cit) fibrinogen (Fib) peptides compared to non-RA controls. We investigated the extent to which these less-studied peptides represent autoantibody targets and sought to visualize the corresponding cit-Fib-reactive B cells in RA patients...
December 1, 2016: Arthritis Research & Therapy
https://www.readbyqxmd.com/read/27903740/protective-t-cell-responses-featured-by-concordant-recognition-of-middle-east-respiratory-syndrome-coronavirus-derived-cd8-t-cell-epitopes-and-host-mhc
#4
William J Liu, Jiaming Lan, Kefang Liu, Yao Deng, Yanfeng Yao, Shaolian Wu, Hong Chen, Lingling Bao, Haifeng Zhang, Min Zhao, Qihui Wang, Lingxia Han, Yan Chai, Jianxun Qi, Jincun Zhao, Songdong Meng, Chuan Qin, George F Gao, Wenjie Tan
The coordinated recognition of virus-derived T cell epitopes and MHC molecules by T cells plays a pivotal role in cellular immunity-mediated virus clearance. It has been demonstrated that the conformation of MHC class I (MHC I) molecules can be adjusted by the presented peptide, which impacts T cell activation. However, it is still largely unknown whether the conformational shift of MHC I influences the protective effect of virus-specific T cells. In this study, utilizing the Middle East respiratory syndrome coronavirus-infected mouse model, we observed that through the unusual secondary anchor Ile5, a CD8(+) T cell epitope drove the conformational fit of Trp(73) on the α1 helix of murine MHC I H-2K(d) In vitro renaturation and circular dichroism assays indicated that this shift of the structure did not influence the peptide/MHC I binding affinity...
November 30, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/27901087/polymorphism-of-fibrillar-structures-depending-on-the-size-of-assembled-a%C3%AE-17-42-peptides
#5
Mookyung Cheon, Mooseok Kang, Iksoo Chang
The size of assembled Aβ17-42 peptides can determine polymorphism during oligomerization and fibrillization, but the mechanism of this effect is unknown. Starting from separate random monomers, various fibrillar oligomers with distinct structural characteristics were identified using discontinuous molecular dynamics simulations based on a coarse-grained protein model. From the structures observed in the simulations, two characteristic oligomer sizes emerged, trimer and paranuclei, which generated distinct structural patterns during fibrillization...
November 30, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27897373/an-integrated-mass-spectrometry-based-approach-to-probe-the-structure-of-the-full-length-wild-type-tetrameric-p53-tumor-suppressor
#6
Christian Arlt, Vanessa Flegler, Christian H Ihling, Mathias Schäfer, Iris Thondorf, Andrea Sinz
We present an integrated approach for investigating the topology of proteins through native mass spectrometry (MS) and cross-linking/MS, which we applied to the full-length wild-type p53 tetramer. For the first time, the two techniques were combined in one workflow to obtain not only structural insight in the p53 tetramer, but also information on the cross-linking efficiency and the impact of cross-linker modification on the conformation of an intrinsically disordered protein (IDP). P53 cross-linking was monitored by native MS and as such, our strategy serves as a quality control for different cross-linking reagents...
November 29, 2016: Angewandte Chemie
https://www.readbyqxmd.com/read/27883268/formation-and-carbon-monoxide-dependent-dissociation-of-allochromatium-vinosum-cytochrome-c-oligomers-using-domain-swapped-dimers
#7
Masaru Yamanaka, Makoto Hoshizumi, Satoshi Nagao, Ryoko Nakayama, Naoki Shibata, Yoshiki Higuchi, Shun Hirota
The number of artificial protein supramolecules has been increasing, however control of protein oligomer formation remains challenging. Cytochrome c' from Allochromatium vinosum (AVCP) is a homo-dimeric protein in its native form, where its protomer exhibits a four-helix bundle structure containing a covalently bound five-coordinate heme as a gas binding site. AVCP exhibits a unique reversible dimer-monomer transition according to the absence and presence of CO. Herein, domain-swapped dimeric AVCP was constructed and utilized to form a tetramer and high-order oligomers...
November 24, 2016: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/27868118/varying-structural-motifs-in-the-salen-based-metal-complexes-of-co-ii-ni-ii-and-cu-ii-synthesis-crystal-structures-molecular-dynamics-and-biological-activities
#8
Anoop Kumar Saini, Pratibha Kumari, Vinay Sharma, Pradeep Mathur, Shaikh M Mobin
Herein, we report the structural and biological activities of a deprotonated Schiff base ligand (H2L) {H2L = 1,1'-(1E,1'E)-(2,4,6-trimethyl-1,3-phenylene)bis(azan-1-yl-1-ylidene)bis(methan-1-yl-1-ylidene)dinaphthalen-2-ol} towards Co(ii), Ni(ii) and Cu(ii) complexes obtained under different conditions. Four new metal complexes have been synthesized: isostructural dimers [Co(L)]2 (1)/[Cu(L)]2 (3), a monomer (Cl3NiH2L)·(Et3NH) (2) and a tetramer [Cu(L)]4 (4). The bioactivity of 1-3 has been investigated through molecular docking with DNA and various proteins, known to be involved in the proliferation of viral diseases or progression of cancer...
November 21, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27867011/tetrameric-acetyl-coa-acetyltransferase-1-is-important-for-tumor-growth
#9
Jun Fan, Ruiting Lin, Siyuan Xia, Dong Chen, Shannon E Elf, Shuangping Liu, Yaozhu Pan, Haidong Xu, Zhiyu Qian, Mei Wang, Changliang Shan, Lu Zhou, Qun-Ying Lei, Yuancheng Li, Hui Mao, Benjamin H Lee, Jessica Sudderth, Ralph J DeBerardinis, Guojing Zhang, Taofeek Owonikoko, Manila Gaddh, Martha L Arellano, Hanna J Khoury, Fadlo R Khuri, Sumin Kang, Paul W Doetsch, Sagar Lonial, Titus J Boggon, Walter J Curran, Jing Chen
Mitochondrial acetyl-CoA acetyltransferase 1 (ACAT1) regulates pyruvate dehydrogenase complex (PDC) by acetylating pyruvate dehydrogenase (PDH) and PDH phosphatase. How ACAT1 is "hijacked" to contribute to the Warburg effect in human cancer remains unclear. We found that active, tetrameric ACAT1 is commonly upregulated in cells stimulated by EGF and in diverse human cancer cells, where ACAT1 tetramers, but not monomers, are phosphorylated and stabilized by enhanced Y407 phosphorylation. Moreover, we identified arecoline hydrobromide (AH) as a covalent ACAT1 inhibitor that binds to and disrupts only ACAT1 tetramers...
December 1, 2016: Molecular Cell
https://www.readbyqxmd.com/read/27865702/biosynthetic-approaches-to-creating-bioactive-fungal-metabolites-pathway-engineering-and-activation-of-secondary-metabolism
#10
REVIEW
Takayuki Motoyama, Hiroyuki Osada
The diversity of natural products is greater than that of combinatorial chemistry compounds and is similar to that of drugs. Compounds rich in sp(3) carbons, such as natural products, typically exhibit high structural complexity and high specificity to molecular targets. Microorganisms can synthesize such sp(3) carbon-rich compounds and can be used as excellent factories for making bioactive compounds. Here, we mainly focus on pathway engineering of two sp(3) carbon-rich bioactive indole alkaloids, fumitremorgin C and terpendole E...
December 15, 2016: Bioorganic & Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/27862429/c-terminal-modification-and-multimerization-increase-the-efficacy-of-a-proline-rich-antimicrobial-peptide
#11
Wenyi Li, Neil M O'Brien-Simpson, Shenggen Yao, Julien Tailhades, Eric C Reynolds, Raymond M Dawson, Laszlo Otvos, Mohammed Akhter Hossain, Frances Separovic, John D Wade
Two series of branched tetramers of the proline-rich antimicrobial peptide (PrAMP), Chex1-Arg20, were prepared to improve antibacterial selectivity and potency against a panel of Gram-negative nosocomial pathogens including Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii and Pseudomonas aeruginosa. First, tetramerization was achieved by dithiomaleimide (DTM) conjugation of two C-terminal-cysteine bearing dimers that also incorporated C-terminal peptide chemical modification. DTM-linked tetrameric peptides containing a C-terminal hydrazide moiety on each dimer exhibited highly potent activities in the minimum inhibitory concentration (MIC) range of 0...
November 16, 2016: Chemistry: a European Journal
https://www.readbyqxmd.com/read/27860539/epitope-specific-antitumor-immunity-suppresses-tumor-spread-in-papillary-thyroid-cancer
#12
Margret Ehlers, Anne Kuebart, Hubertus Hautzel, Juergen Enczmann, Anna-Carinna Reis, Matthias Haase, Stephanie Allelein, Till Dringenberg, Christine Schmid, Matthias Schott
CONTEXT: Papillary thyroid cancer (PTC) is characterized by a lymphocytic infiltration. PTC patients with lymphocytic infiltration may have a better clinical outcome. OBJECTIVE: Characterization of tumor epitope-specific immunity and correlation analyses with the clinical outcome. PATIENTS: 150 PTC patients; 40 Hashimoto's thyroiditis (HT) patients; 21 healthy controls; 27.239 healthy Caucasians (for HLA typing). MAIN OUTCOME MEASURES: HLA class I restricted thyroperoxidase (TPO) and thyroglobulin (Tg) epitope-specific T cells (tetramer analyses), correlation analyses between HLA class II phenotypes, T cell immunity, and the clinical course...
November 18, 2016: Journal of Clinical Endocrinology and Metabolism
https://www.readbyqxmd.com/read/27859859/oligomerization-domains-in-the-glycan-binding-receptors-dc-sign-and-dc-signr-sequence-variation-and-stability-differences
#13
Ália Dos Santos, Andreas Hadjivasiliou, Felipe Ossa, Novandy K Lim, Aylin Turgut, Maureen E Taylor, Kurt Drickamer
Human dendritic cell-specific intercellular adhesion molecule-1 grabbing nonintegrin, DC-SIGN, and the sinusoidal endothelial cell receptor DC-SIGNR or L-SIGN, are closely related sugar-binding receptors. DC-SIGN acts both as a pathogen-binding endocytic receptor and as a cell adhesion molecule, while DC-SIGNR has only the pathogen-binding function. In addition to differences in the sugar-binding properties of the carbohydrate-recognition domains in the two receptors, there are sequence differences in the adjacent neck domains, which are coiled-coil tetramerization domains comprised largely of 23-amino acid repeat units...
November 17, 2016: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/27856006/insights-into-the-aggregation-mechanism-of-a%C3%AE-25-40
#14
Jian Xiong, Renee D JiJi
The hydrophobic fragment of the Alzheimer's related β-amyloid (Aβ) peptide, Aβ(25-40), aggregates and forms insoluble amyloid fibrils at a rate similar to the full-length peptide. In order to gain insight into the fibrillization of Aβ(25-40) and the ability of the flavonoid myricetin to inhibit its aggregation, the isoleucine at position 32 (I32A) and the glycine at position 37 (G37A) in the full-length peptide were replaced with alanine. Thioflavin T assays indicate that substitution of isoleucine for alanine significantly reduces the rate and extent of fibrillization compared to the Aβ(25-40) and G37A peptides...
January 2017: Biophysical Chemistry
https://www.readbyqxmd.com/read/27854016/histone-purification-from-saccharomyces-cerevisiae
#15
Frederic Jourquin, Vincent Géli
The nucleosome structure consists of a histone octamer made by a tetramer of H3-H4 histones and two dimers of H2A-H2B. Nucleosomes undergo extensive posttranslational modifications that regulate nucleosome interactions, position, and stability.We describe a protocol allowing the robust purification of histones from the yeast Saccharomyces cerevisiae. This method appears to be suitable to quantitatively analyze specific posttranslational histone modifications.
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27853786/identification-and-isolation-of-antigen-specific-cytotoxic-t-lymphocytes-with-an-automated-microraft-sorting-system
#16
Peter J Attayek, Sally A Hunsucker, Christopher E Sims, Nancy L Allbritton, Paul M Armistead
The simultaneous measurement of T cell function with recovery of individual T cells would greatly facilitate characterizing antigen-specific responses both in vivo and in model systems. We have developed a microraft array methodology that automatically measures the ability of individual T cells to kill a population of target cells and viably sorts specific cells into a 96-well plate for expansion. A human T cell culture was generated against the influenza M1p antigen. Individual microrafts on a 70 × 70 array were loaded with on average 1 CD8(+) cell from the culture and a population of M1p presenting target cells...
November 17, 2016: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/27853286/disulfide-mapping-the-voltage-sensing-mechanism-of-a-voltage-dependent-potassium-channel
#17
Tomohiro Nozaki, Shin-Ichiro Ozawa, Hitomi Harada, Tomomi Kimura, Masanori Osawa, Ichio Shimada
Voltage-dependent potassium (Kv) channels allow for the selective permeability of potassium ions in a membrane potential dependent manner, playing crucial roles in neurotransmission and muscle contraction. Kv channel is a tetramer, in which each subunit possesses a voltage-sensing domain (VSD) and a pore domain (PD). Although several lines of evidence indicated that membrane depolarization is sensed as the movement of helix S4 of the VSD, the detailed voltage-sensing mechanism remained elusive, due to the difficulty of structural analyses at resting potential...
November 17, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27851957/conserved-tetramer-junction-in-the-kinetochore-ndc80-complex
#18
Roberto Valverde, Jessica Ingram, Stephen C Harrison
The heterotetrameric Ndc80 complex establishes connectivity along the principal longitudinal axis of a kinetochore. Its two heterodimeric subcomplexes, each with a globular end and a coiled-coil shaft, connect end-to-end to create a ∼600 Å long rod spanning the gap from centromere-proximal structures to spindle microtubules. Neither subcomplex has a known function on its own, but the heterotetrameric organization and the characteristics of the junction are conserved from yeast to man. We have determined crystal structures of two shortened ("dwarf") Ndc80 complexes that contain the full tetramer junction and both globular ends...
November 15, 2016: Cell Reports
https://www.readbyqxmd.com/read/27849628/phase-diagrams-of-vortex-matter-with-multi-scale-inter-vortex-interactions-in-layered-superconductors
#19
Qingyou Meng, Christopher N Varney, Hans Fangohr, Egor Babaev
It was recently proposed to use the stray magnetic fields of superconducting vortex lattices to trap ultracold atoms for building quantum emulators. This calls for new methods for engineering and manipulating of the vortex states. One of the possible routes utilizes type-1.5 superconducting layered systems with multi-scale inter-vortex interactions. In order to explore the possible vortex states that can be engineered, we present two phase diagrams of phenomenological vortex matter models with multi-scale inter-vortex interactions featuring several attractive and repulsive length scales...
November 16, 2016: Journal of Physics. Condensed Matter: An Institute of Physics Journal
https://www.readbyqxmd.com/read/27849172/novel-e2-glycoprotein-tetramer-detects-hepatitis-c-virus-specific-memory-b-cells
#20
Maude Boisvert, Wanrui Zhang, Elizabeth J Elrod, Nicole F Bernard, Jean-Pierre Villeneuve, Julie Bruneau, Joseph Marcotrigiano, Naglaa H Shoukry, Arash Grakoui
Acute hepatitis C virus (HCV) infection culminates in viral persistence in the majority of cases. Abs that recognize the envelope glycoproteins E1 and E2 are generated during the late stages of acute infection, yet their contribution to spontaneous viral clearance remains controversial. Investigation of the humoral responses during acute HCV infection have been limited by the inability to directly identify and characterize HCV-specific B cells. In this study we describe the development of a novel tetramer of the E2 glycoprotein ectodomain (J6, genotype 2a strain), which allowed us to visualize E2-specific B cells longitudinally in the peripheral blood of HCV-infected individuals...
December 15, 2016: Journal of Immunology: Official Journal of the American Association of Immunologists
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