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JOURNAL ARTICLE
Yan Mao, Xinye Li, Rui Ren, Yue Yuan, Li Wang, Xuehong Zhang
OBJECTIVE: Preeclampsia (PE) is a severe complication of unclear pathogenesis associated with pregnancy. This research aimed to elucidate the properties of immune cell infiltration and potential biomarkers of PE based on bioinformatics analysis. MATERIALS AND METHODS: Two PE datasets were imported from the Gene ExpressioOmnibus (GEO) and screened to identify differentially expressed genes (DEGs). Significant module genes were identified by weighted gene co-expression network analysis (WGCNA)...
2024: PloS One
#2
JOURNAL ARTICLE
Xiuyuan Lu, Hiroki Hayashi, Eri Ishikawa, Yukiko Takeuchi, Julian Vincent Tabora Dychiao, Hironori Nakagami, Sho Yamasaki
SARS-CoV-2 vaccines have been used worldwide to combat COVID-19 pandemic. To elucidate the factors that determine the longevity of spike (S)-specific antibodies, we traced the characteristics of S-specific T cell clonotypes together with their epitopes and anti-S antibody titers before and after BNT162b2 vaccination over time. T cell receptor (TCR) αβ sequences and mRNA expression of the S-responded T cells were investigated using single-cell TCR- and RNA-sequencing. Highly expanded 199 TCR clonotypes upon stimulation with S peptide pools were reconstituted into a reporter T cell line for the determination of epitopes and restricting HLAs...
May 8, 2024: ELife
#3
JOURNAL ARTICLE
Nicholas You Zhi Cheang, Kai Sen Tan, Peck Szee Tan, Kiren Purushotorma, Wee Chee Yap, Kirsteen Mcinnes Tullett, Benson Yen Leong Chua, Aileen Ying-Yan Yeoh, Caris Qi Hui Tan, Xinlei Qian, Huixin Chen, Douglas Jie Wen Tay, Irina Caminschi, Yee Joo Tan, Paul Anthony Macary, Chee Wah Tan, Mireille Hanna Lahoud, Sylvie Alonso
Current COVID-19 vaccines face limitations including waning immunity, immune escape by SARS-CoV-2 variants, limited cellular response, and poor mucosal immunity. We engineered a Clec9A-Receptor Binding Domain (RBD) antibody construct that delivers the SARS-CoV-2 RBD to conventional type 1 dendritic cells. Compared to non-targeting approaches, single dose immunization in mice with Clec9A-RBD induced far higher RBD-specific antibody titers that were sustained for up to 21 months post-vaccination. Uniquely, increasing neutralizing and antibody-dependent cytotoxicity activities across the sarbecovirus family was observed, suggesting antibody affinity maturation over time...
May 6, 2024: Molecular Therapy
#4
JOURNAL ARTICLE
Zhenqi Gong, Qing Zheng, Baizhi Li, Huaiming Wang, Hongwu Chen, Shaoxiong Lin
Background: Recently, various evidence has confirmed that Tyrosine Kinase with Immunoglobulin-like and EGF-like domains 1 (TIE1) promotes tumor growth in many cancers. However, the precise mechanism underlying TIE1's involvement in Gastric Cancer (GC) remains elusive. This research aimed to investigate the biological function of TIE1 in regulating GC progression. Methods: The Cancer Genome Atlas (TCGA), Gene Expression Omnibus (GEO), GEPIA2.0, Sangerbox3.0 and TIMER databases were used to analyze the TIE1 expression...
2024: Journal of Cancer
#5
JOURNAL ARTICLE
Mehreen Elahee, Alisa A Mueller, Runci Wang, Kathryne E Marks, Takanori Sasaki, Ye Cao, Andrea Fava, Paul F Dellaripa, Francesco Boin, Deepak A Rao
OBJECTIVE: T cells contribute to tissue injury in systemic sclerosis (SSc), yet the specific T cell subsets expanded in patients with SSc remain incompletely defined. Here we evaluated specific phenotypes and functions of peripheral helper T (Tph) and follicular helper T (Tfh) cells, which have been implicated in autoantibody production, and assessed their associations with clinical features in a well-characterized cohort of patients with SSc. METHODS: Mass cytometry of T cells from peripheral blood mononuclear cells of patients with SSc and controls were evaluated using t-distributed stochastic neighbor embedding visualization, biaxial gating, and marker expression levels...
May 3, 2024: ACR open rheumatology
#6
JOURNAL ARTICLE
Cèlia Dobaño-López, Juan García Valero, Ferran Araujo-Ayala, Ferran Nadeu, Fabien Gava, Carla Faria, Marine Norlund, Renaud Morin, Pascale Bernes-Lasserre, Fabian Arenas, Marta Grau, Cristina López, Irene López-Oreja, Neus Serrat, Ares Martínez-Farran, Lluís Hernández, Heribert Playa-Albinyana, Rubén Giménez, Silvia Beà, Elías Campo, Jean-Michel Lagarde, Armando López-Guillermo, Laura Magnano, Dolors Colomer, Christine Bezombes, Patricia Pérez-Galán
Follicular lymphoma (FL), the most common indolent non-Hodgkin lymphoma, constitutes a paradigm of immune tumor microenvironment (TME) contribution to disease onset, progression, and heterogenous clinical outcome. Here we present the first FL-Patient Derived Lymphoma Spheroid (FL-PDLS), including fundamental immune actors and features of TME in FL lymph nodes (LNs). FL-PDLS is organized in disc-shaped 3D structures composed of proliferating B and T cells, together with macrophages with an intermediate M1/M2 phenotype...
May 2, 2024: Blood Cancer Journal
#7
JOURNAL ARTICLE
Jian Lu, Huimin Zhou, Yuxuan Chen, Xueli Xia, Jun Yang, Jie Ma, Jie Tian, Shengjun Wang
Soluble components secreted by Tfh cells are critical for the germinal center responses. In this study, we investigated whether Tfh cells could regulate the B-cell response by releasing small extracellular vesicles (sEVs). Our results showed that Tfh cells promote B-cell differentiation and antibody production through sEVs and that CD40L plays a crucial role in Tfh-sEVs function. In addition, increased Tfh-sEVs were found in mice with collagen-induced arthritis (CIA). Adoptive transfer of Tfh cells significantly exacerbated the severity of CIA; however, the effect of Tfh cells on exacerbating the CIA process was significantly diminished after inhibiting sEVs secretion...
May 1, 2024: Journal of Autoimmunity
#8
JOURNAL ARTICLE
Hitoshi Kawasuji, Yoshitomo Morinaga, Kentaro Nagaoka, Hideki Tani, Yoshihiro Yoshida, Hiroshi Yamada, Yusuke Takegoshi, Makito Kaneda, Yushi Murai, Kou Kimoto, Hideki Niimi, Yoshihiro Yamamoto
BACKGROUND: Acute immune responses to coronavirus disease 2019 (COVID-19) are influenced by variants, vaccination, and clinical severity. Thus, the outcome of these responses may differ between vaccinated and unvaccinated patients and those with and without COVID-19-related pneumonia. In this study, these differences during infection with the Omicron variant were investigated. METHODS: A total of 67 patients (including 47 vaccinated and 20 unvaccinated patients) who were hospitalized within 5 days after COVID-19 symptom onset were enrolled in this prospective observational study...
2024: Frontiers in Immunology
#9
JOURNAL ARTICLE
Lauren E Higdon, Sheila Scheiding, Anna M Kus, Noha Lim, S Alice Long, Mark S Anderson, Alice E Wiedeman
Peripheral blood mononuclear cell (PBMC) immunophenotyping is crucial in tracking activation, disease state, and response to therapy in human subjects. Many studies require shipping of blood from clinical sites to a laboratory for processing to PBMC, which can lead to delays that impact sample quality. We used an extensive cytometry by time-of-flight (CyTOF) immunophenotyping panel to analyze the impacts of delays to processing and distinct storage conditions on cell composition and quality of PBMC from seven adults across a range of ages, including two with rheumatoid arthritis...
May 2, 2024: Clinical and Experimental Immunology
#10
JOURNAL ARTICLE
Hongkui Xiao, Isabel Ulmert, Luisa Bach, Johanna Huber, Hamsa Narasimhan, Ilia Kurochkin, Yinshui Chang, Signe Holst, Urs Mörbe, Lili Zhang, Andreas Schlitzer, Carlos-Filipe Pereira, Barbara U Schraml, Dirk Baumjohann, Katharina Lahl
Conventional dendritic cells (cDC) play key roles in immune induction, but what drives their heterogeneity and functional specialization is still ill-defined. Here we show that cDC-specific deletion of the transcriptional repressor Bcl6 in mice alters the phenotype and transcriptome of cDC1 and cDC2, while their lineage identity is preserved. Bcl6-deficient cDC1 are diminished in the periphery but maintain their ability to cross-present antigen to CD8+ T cells, confirming general maintenance of this subset...
April 30, 2024: Nature Communications
#11
JOURNAL ARTICLE
Yifei Liao, Jinjie Yan, Nina R Beri, Lisa G Roth, Ethel Cesarman, Benjamin E Gewurz
Epstein-Barr virus (EBV) persistently infects 95% of adults worldwide and is associated with multiple human lymphomas that express characteristic EBV latency programs used by the virus to navigate the B-cell compartment. Upon primary infection, the EBV latency III program, comprised of six Epstein-Barr Nuclear Antigens (EBNA) and two Latent Membrane Protein (LMP) antigens, drives infected B-cells into germinal center (GC). By incompletely understood mechanisms, GC microenvironmental cues trigger the EBV genome to switch to the latency II program, comprised of EBNA1, LMP1 and LMP2A and observed in GC-derived Hodgkin lymphoma...
April 29, 2024: PLoS Pathogens
#12
JOURNAL ARTICLE
Mijung Yoon, Yurim Choi, Taeuk Wi, Youn Soo Choi, Jinyong Choi
Germinal center (GC) responses are essential for establishing protective, long-lasting immunity through the differentiation of GC B cells (BGC ) and plasma cells (BPC ), along with the generation of antigen-specific antibodies. Among the various pathways influencing immune responses, the STING (Stimulator of Interferon Genes) pathway has emerged as significant, especially in innate immunity, and extends its influence to adaptive responses. In this study, we examined how the STING ligand cGAMP can modulate these key elements of the adaptive immune response, particularly in enhancing GC reactions and the differentiation of BGC , BPC , and follicular helper T cells (TFH )...
2024: Frontiers in Immunology
#13
JOURNAL ARTICLE
Yutaro Nasa, Atsushi Satake, Ryohei Tsuji, Ryo Saito, Yukie Tsubokura, Hideaki Yoshimura, Tomoki Ito
INTRODUCTION: Defective interleukin-2 (IL-2) production contributes to immune system imbalance in patients with systemic erythematosus lupus (SLE). Recent clinical studies suggested that low-dose IL-2 treatment is beneficial for SLE and the therapeutic effect is associated with regulatory T cell (Treg) expansion. Pharmacological calcineurin inhibition induces a reduction in the number of Tregs because they require stimulation of T cell receptor signaling and IL-2 for optimal proliferation...
2024: Frontiers in Immunology
#14
JOURNAL ARTICLE
Jialing Wu, Xi Zhang, Shen Lin, Qiujing Wei, Zhiming Lin, Ou Jin, Jieruo Gu
OBJECTIVES: To determine the alteration of peripheral T and B cell subsets in patients with systemic sclerosis (SSc) and to evaluate their correlation with the progression of SSc. METHODS: We recruited 47 SSc patients and 45 healthy controls (HCs) in this study. Demographic and clinical data were then collected. Flow cytometry was used to detect the proportions of 44 different T and B cell subsets in circulating blood. RESULTS: The proportion of total B cells (p = ...
April 2024: International Journal of Rheumatic Diseases
#15
Catherine S Forconi, Christina Nixon, Hannah W Wu, Boaz Odwar, Sunthorn Pond-Tor, John M Ong'echa, Jonathan Kurtis, Ann M Moormann
BACKGROUND: Circulating T-follicular helper (cT FH ) cells have the potential to provide an additional correlate of protection against Plasmodium falciparum ( Pf) as they are essential to promote B cell production of long-lasting antibodies. Assessing the specificity of cT FH subsets to individual malaria antigens is vital to understanding the variation observed in antibody responses and identifying promising malaria vaccine candidates. METHODS: Using spectral flow cytometry and unbiased clustering analysis we assessed antigen-specific cT FH cell recall responses in vitro to malaria vaccine candidates Pf SEA-1A and Pf GARP within a cross-section of children and adults living in a malaria holoendemic region of western Kenya...
April 16, 2024: bioRxiv
#16
JOURNAL ARTICLE
Dylan T Ammons, Leone S Hopkins, Kathryn E Cronise, Jade Kurihara, Daniel P Regan, Steven Dow
Osteosarcoma (OS) is a heterogeneous, aggressive malignancy of the bone that disproportionally affects children and adolescents. Therapeutic interventions for OS are limited, which is in part due to the complex tumor microenvironment (TME). As such, we used single-cell RNA sequencing (scRNA-seq) to describe the cellular and molecular composition of the TME in 6 treatment-naïve dogs with spontaneously occurring primary OS. Through analysis of 35,310 cells, we identified 41 transcriptomically distinct cell types including the characterization of follicular helper T cells, mature regulatory dendritic cells (mregDCs), and 8 tumor-associated macrophage (TAM) populations...
April 24, 2024: Communications Biology
#17
JOURNAL ARTICLE
Harini Bagavant, Justyna Durslewicz, Marcelina Pyclik, Magdalena Makuch, Joanna A Papinska, Umesh S Deshmukh
Sjögren's disease (SjD), characterized by circulating autoantibodies and exocrine gland inflammation, is typically diagnosed in women over 50 years of age. However, the contribution of age to SjD pathogenesis is unclear. C57BL/6 female mice at different ages were studied to investigate how aging influences the dynamics of salivary gland inflammation. Salivary glands were characterized for immune cell infiltration, inflammatory gene expression, and saliva production. At 8 months, gene expression of several chemokines involved in immune cell trafficking was significantly elevated...
April 24, 2024: GeroScience
#18
JOURNAL ARTICLE
Yuki Hitomi, Kazuko Ueno, Yoshihiro Aiba, Nao Nishida, Michihiro Kono, Mitsuki Sugihara, Yosuke Kawai, Minae Kawashima, Seik-Soon Khor, Kazuhiro Sugi, Hirotaka Kouno, Hiroshi Kouno, Atsushi Naganuma, Satoru Iwamoto, Shinji Katsushima, Kiyoshi Furuta, Toshiki Nikami, Tomohiko Mannami, Tsutomu Yamashita, Keisuke Ario, Tatsuji Komatsu, Fujio Makita, Masaaki Shimada, Noboru Hirashima, Shiro Yokohama, Hideo Nishimura, Rie Sugimoto, Takuya Komura, Hajime Ota, Motoyuki Kojima, Makoto Nakamuta, Naoyuki Fujimori, Kaname Yoshizawa, Yutaka Mano, Hironao Takahashi, Kana Hirooka, Satoru Tsuruta, Takeaki Sato, Kazumi Yamasaki, Yuki Kugiyama, Yasuhide Motoyoshi, Tomoyuki Suehiro, Akira Saeki, Kosuke Matsumoto, Shinya Nagaoka, Seigo Abiru, Hiroshi Yatsuhashi, Masahiro Ito, Kazuhito Kawata, Akinobu Takaki, Kuniaki Arai, Teruko Arinaga, Masanori Abe, Masaru Harada, Makiko Taniai, Mikio Zeniya, Hiromasa Ohira, Shinji Shimoda, Atsumasa Komori, Atsushi Tanaka, Kazuyoshi Ishigaki, Masao Nagasaki, Katsushi Tokunaga, Minoru Nakamura
BACKGROUND AIMS: Previous genome-wide association studies (GWAS) have indicated the involvement of shared (population-non-specific) and non-shared (population-specific) susceptibility genes in the pathogenesis of primary biliary cholangitis (PBC) among European and East-Asian populations. Although a meta-analysis of these distinct populations has recently identified more than 20 novel PBC susceptibility loci, analyses of population-specific genetic architecture are still needed for a more comprehensive search for genetic factors in PBC...
April 23, 2024: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
#19
JOURNAL ARTICLE
Lin Cong, Yijie He, Yun Wu, Ze Li, Siwen Ding, Weiwei Liang, Xingjun Xiao, Huixue Zhang, Lihua Wang
BACKGROUND: Stroke is a disease with high morbidity, disability, and mortality. Immune factors play a crucial role in the occurrence of ischemic stroke (IS), but their exact mechanism is not clear. This study aims to identify possible immunological mechanisms by recognizing immune-related biomarkers and evaluating the infiltration pattern of immune cells. METHODS: We downloaded datasets of IS patients from GEO, applied R language to discover differentially expressed genes, and elucidated their biological functions using GO, KEGG analysis, and GSEA analysis...
2024: PeerJ
#20
JOURNAL ARTICLE
Rashidul Islam, Jannis Heyer, Miriam Figura, Xiaoyan Wang, Xichen Nie, Benedict Nathaniel, Sivanjah Indumathy, Katja Hartmann, Christiane Pleuger, Monika Fijak, Sabine Kliesch, Florian Dittmar, Adrian Pilatz, Florian Wagenlehner, Mark Hedger, Bruce Loveland, James H Hotaling, Jingtao Guo, Kate L Loveland, Hans-Christian Schuppe, Daniela Fietz
BACKGROUND: Immune cell infiltration is heterogeneous but common in testicular germ cell tumors (TGCT) and pre-invasive germ cell neoplasia in situ (GCNIS). Tumor-infiltrating T cells including regulatory T (Treg) and follicular helper T (Tfh) cells are found in other cancer entities, but their contributions to TGCT are unknown. METHODS: Human testis specimens from independent patient cohorts were analyzed using immunohistochemistry, flow cytometry and single-cell RNA sequencing (scRNA-seq) with special emphasis on delineating T cell subtypes...
April 22, 2024: British Journal of Cancer
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