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histone chaperone

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https://www.readbyqxmd.com/read/29773913/auxin-decreases-chromatin-accessibility-through-the-tir1-afbs-auxin-signaling-pathway-in-proliferative-cells
#1
Junko Hasegawa, Takuya Sakamoto, Satoru Fujimoto, Tomoe Yamashita, Takamasa Suzuki, Sachihiro Matsunaga
Chromatin accessibility is closely associated with chromatin functions such as gene expression, DNA replication, and maintenance of DNA integrity. However, the relationship between chromatin accessibility and plant hormone signaling has remained elusive. Here, based on the correlation between chromatin accessibility and DNA damage, we used the sensitivity to DNA double strand breaks (DSBs) as an indicator of chromatin accessibility and demonstrated that auxin regulates chromatin accessibility through the TIR1/AFBs signaling pathway in proliferative cells...
May 17, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29771637/quantitative-imaging-of-chromatin-decompaction-in-living-cells
#2
Elisa Dultz, Roberta Mancini, Guido Polles, Pascal Vallotton, Frank Alber, Karsten Weis
Chromatin organization is highly dynamic and regulates transcription. Upon transcriptional activation, chromatin is remodeled and referred to as "open", but quantitative and dynamic data of this decompaction process are lacking. Here, we have developed a quantitative high-resolution microscopy assay in living yeast cells to visualize and quantify chromatin dynamics using the GAL7-10-1 locus as a model system. Upon transcriptional activation of these three clustered genes, we detect an increase of the mean distance across this locus by >100 nm...
May 17, 2018: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/29764934/structure-specific-recognition-protein-1-ssrp1-is-an-elongated-homodimer-that-binds-histones
#3
Gabriele Marcianò, Stefano Da Vela, Giancarlo Tria, Dmitri I Svergun, Olwyn Byron, Danny T Huang
The histone chaperone complex facilitates chromatin transcription (FACT) plays important roles in DNA repair, replication, and transcription. In the formation of this complex, structure-specific recognition protein-1 (SSRP1) heterodimerizes with suppressor of Ty 16 (SPT16). SSRP1 also has SPT16-independent functions, but how SSRP1 functions alone remains elusive. Here, using analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS) techniques, we characterized human SSRP1 and that from the amoeba Dictyostelium discoideum and show that both orthologs form an elongated homodimer in solution...
May 15, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29751847/asf1-is-required-to-load-histones-on-the-hira-complex-in-preparation-of-paternal-chromatin-assembly-at-fertilization
#4
Béatrice Horard, Laure Sapey-Triomphe, Emilie Bonnefoy, Benjamin Loppin
BACKGROUND: Anti-Silencing Factor 1 (ASF1) is a conserved H3-H4 histone chaperone involved in both Replication-Coupled and Replication-Independent (RI) nucleosome assembly pathways. At DNA replication forks, ASF1 plays an important role in regulating the supply of H3.1/2 and H4 to the CAF-1 chromatin assembly complex. ASF1 also provides H3.3-H4 dimers to HIRA and DAXX chaperones for RI nucleosome assembly. The early Drosophila embryo is an attractive system to study chromatin assembly in a developmental context...
May 11, 2018: Epigenetics & Chromatin
https://www.readbyqxmd.com/read/29746960/oligomers-of-human-histone-chaperone-npm1-alter-p300-kat3b-folding-to-induce-autoacetylation
#5
Stephanie Kaypee, Smitha Asoka Sahadevan, Deepthi Sudarshan, Sarmistha Halder Sinha, Shilpa Patil, Parijat Senapati, Gopinath S Kodaganur, Azeem Mohiyuddin, Dipak Dasgupta, Tapas K Kundu
BACKGROUND: p300 (KAT3B) lysine acetyltransferase activity is modulated under different physiological and pathological contexts through the induction of trans-autoacetylation. This phenomenon is mediated by several factors, mechanisms of which are not fully understood. METHODS: Through acetyltransferase assays using full-length, baculovirus-expressed KATs, the specificity of NPM1-mediated enhancement of p300 autoacetylation was tested. Chaperone assays and tryptophan fluorescence studies were performed to evaluate the NPM1-induced protein folding...
May 7, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29743473/increased-expression-of-holliday-junction-recognizing-protein-hjurp-as-an-independent-prognostic-biomarker-in-advanced-stage-serous-ovarian-carcinoma
#6
Lin Li, Xiaoli Li, Qin Meng, Abdul Qadir Khan, Xiaomei Chen
BACKGROUND Advanced-stage serous ovarian carcinoma results in the majority of deaths from ovarian carcinoma. The histone chaperone, Holliday junction-recognizing protein (HJURP), binds with centromere protein-A (CENP-A) and its expression has been shown to be a prognostic biomarker in some cancers. The aim of this study was to investigate the role of HJURP expression in advanced-stage serous ovarian carcinoma. MATERIAL AND METHODS Ninety-eight patients with advanced-stage serous ovarian carcinoma, who had tumor tissue samples available, were studied...
May 10, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29717210/exploring-intrinsically-disordered-proteins-in-chlamydomonas-reinhardtii
#7
Zhang Yizhi, Launay Hélène, Schramm Antoine, Lebrun Régine, Gontero Brigitte
The content of intrinsically disordered protein (IDP) is related to organism complexity, evolution, and regulation. In the Plantae, despite their high complexity, experimental investigation of IDP content is lacking. We identified by mass spectrometry 682 heat-resistant proteins from the green alga, Chlamydomonas reinhardtii. Using a phosphoproteome database, we found that 331 of these proteins are targets of phosphorylation. We analyzed the flexibility propensity of the heat-resistant proteins and their specific features as well as those of predicted IDPs from the same organism...
May 1, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29698889/acetylation-of-oxidized-base-repair-initiating-neil1-dna-glycosylase-required-for-chromatin-bound-repair-complex-formation-in-the-human-genome-increases-cellular-resistance-to-oxidative-stress
#8
Shiladitya Sengupta, Chunying Yang, Muralidhar L Hegde, Pavana M Hegde, Joy Mitra, Arvind Pandey, Arijit Dutta, Abdul Tayyeb Datarwala, Kishor K Bhakat, Sankar Mitra
Posttranslational modifications of DNA repair proteins have been linked to their function. However, it is not clear if posttranslational acetylation affects subcellular localization of these enzymes. Here, we show that the human DNA glycosylase NEIL1, which is involved in repair of both endo- and exogenously generated oxidized bases via the base excision repair (BER) pathway, is acetylated by histone acetyltransferase p300. Acetylation occurs predominantly at Lys residues 296, 297 and 298 located in NEIL1's disordered C-terminal domain...
April 17, 2018: DNA Repair
https://www.readbyqxmd.com/read/29695490/acetylation-dependent-recruitment-of-the-fact-complex-and-its-role-in-regulating-pol-ii-occupancy-genome-wide-in-saccharomyces-cerevisiae
#9
Rakesh Pathak, Priyanka Singh, Sudha Ananthakrishnan, Sarah Adamczyk, Olivia Schimmel, Chhabi K Govind
Histone chaperones, chromatin remodelers, and histone modifying complexes play a critical role in alleviating the nucleosomal barrier for DNA-dependent processes. Here, we have examined the role of two highly conserved yeast ( Saccharomyces cerevisiae ) histone chaperones, FACT and Spt6, in regulating transcription. We show that the H3 tail contributes to the recruitment of FACT to coding sequences in a manner dependent on acetylation. We found that deleting a H3 HAT Gcn5 or mutating lysines on the H3 tail impairs FACT recruitment at ADH1 and ARG1 genes...
April 25, 2018: Genetics
https://www.readbyqxmd.com/read/29676825/histone-chaperones-play-crucial-roles-in-maintenance-of-stem-cell-niche-during-plant-root-development
#10
Jing Ma, Yuhao Liu, Wangbin Zhou, Yan Zhu, Aiwu Dong, Wen-Hui Shen
Stem cells in both plant and animal kingdoms reside in a specialized cellular context called stem-cell niche (SCN). SCN integrity is crucial for organism development. Here we show that the H3/H4 histone chaperone CHROMATIN ASSEMBLY FACTOR-1 (CAF-1) and the H2A/H2B histone chaperone NAP1-RELATED PROTEIN1/2 (NRP1/2) play synergistic roles in Arabidopsis root SCN maintenance. Compared to either the m56-1 double mutant deprived of NRP1 and NRP2 or the fas2-4 mutant deprived of CAF-1, the combined m56-1fas2-4 triple mutant displayed a much more severe short-root phenotype...
April 20, 2018: Plant Journal: for Cell and Molecular Biology
https://www.readbyqxmd.com/read/29674330/correction-histone-chaperone-aplf-regulates-induction-of-pluripotency-in-murine-fibroblasts-doi-10-1242-jcs-194035
#11
Khaja Mohieddin Syed, Sunu Joseph, Ananda Mukherjee, Aditi Majumder, Jose M Teixeira, Debasree Dutta, Madhavan Radhakrishna Pillai
No abstract text is available yet for this article.
April 19, 2018: Journal of Cell Science
https://www.readbyqxmd.com/read/29665050/silence-of-hdac6-suppressed-esophageal-squamous-cell-carcinoma-proliferation-and-migration-by-disrupting-chaperone-function-of-hsp90
#12
Hua Tao, Yuan-Yuan Chen, Zong-Wen Sun, Hua-Lin Chen, Ming Chen
Esophageal carcinoma is aggressive in nature and its prognosis is largely dependent on the degree of invasion. Histone deacetylase 6 (HDAC6), as the most unique member of HDACs family, has the positive activity to promote initiation and progression of various cancers via targeting multiple non-histone proteins in cytoplasm. In this study, we found that HDAC6 was over-expressed in three esophageal cancer cell lines (KYSE140, KYSE170, KYSE180) when compared to non-carcinoma esophageal epithelial cell HEEC-1. Then two HDAC6 specific siRNAs and HDAC6 inhibitor tubastatin A greatly suppressed KYSE140 and KYSE180 cells proliferation and migration, and the inhibition of cell motility was accompanied by elevated acetylation of α-tubulin, a target of HDAC6...
April 17, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29661843/functional-analysis-of-hif1-histone-chaperone-in-saccharomyces-cerevisiae
#13
Nora S Dannah, Syed Nabeel-Shah, Christoph F Kurat, Sarah A Sabatinos, Jeffrey Fillingham
The Hif1 protein in the yeast Saccharomyces cerevisie is an evolutionarily conserved H3/H4-specific chaperone and a subunit of the nuclear Hat1 complex that catalyzes the acetylation of newly synthesized histone H4. Hif1, as well as its human homolog NASP, has been implicated in an array of chromatin-related processes including histone H3/H4 transport, chromatin assembly and DNA repair. In this study, we elucidate the functional aspects of Hif1. Initially we establish the wide distribution of Hif1 homologs with an evolutionarily conserved pattern of four tetratricopeptide repeats (TPR) motifs throughout the major fungal lineages and beyond...
April 16, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29643123/intragenic-transcriptional-interference-regulates-the-human-immune-ligand-mica
#14
Da Lin, Thomas K Hiron, Christopher A O'Callaghan
Many human genes have tandem promoters driving overlapping transcription, but the value of this distributed promoter configuration is generally unclear. Here we show that MICA , a gene encoding a ligand for the activating immune receptor NKG2D, contains a conserved upstream promoter that expresses a noncoding transcript. Transcription from the upstream promoter represses the downstream standard promoter activity in cis through transcriptional interference. The effect of transcriptional interference depends on the strength of transcription from the upstream promoter and can be described quantitatively by a simple reciprocal repressor function...
April 11, 2018: EMBO Journal
https://www.readbyqxmd.com/read/29599486/usp52-acts-as-a-deubiquitinase-and-promotes-histone-chaperone-asf1a-stabilization
#15
Shangda Yang, Ling Liu, Cheng Cao, Nan Song, Yuejiao Wang, Shuai Ma, Qi Zhang, Na Yu, Xiang Ding, Fuquan Yang, Shanshan Tian, Kai Zhang, Tao Sun, Jie Yang, Zhi Yao, Shaoyuan Wu, Lei Shi
Histone chaperone ASF1A has been reported to be dysregulated in multiple tumors; however, the underlying molecular mechanism that how the abundance and function of ASF1A are regulated remains unclear. Here we report that ASF1A is physically associated with USP52, which is previously identified as a pseudo-deubiquitinase. Interestingly, we demonstrate that USP52 is a bona fide ubiquitin-specific protease, and USP52 promotes ASF1A deubiquitination and stabilization. USP52-promoted ASF1A stabilization facilitates chromatin assembly and favors cell cycle progression...
March 29, 2018: Nature Communications
https://www.readbyqxmd.com/read/29599176/h3k4-methylation-dependent-and-independent-chromatin-regulation-by-jhd2-and-set1-in-budding-yeast
#16
Kwan Yin Lee, Ziyan Chen, River Jiang, Marc D Meneghini
Set1 and Jhd2 regulate the methylation state of histone H3 lysine-4 (H3K4me) through their opposing methyltransferase and demethylase activities in the budding yeast Saccharomyces cerevisiae H3K4me associates with actively transcribed genes and, like both SET1 and JHD2 themselves, is known to regulate gene expression diversely. It remains unclear, however, if Set1 and Jhd2 act solely through H3K4me. Relevantly, Set1 methylates lysine residues in the kinetochore protein Dam1 while genetic studies of the S. pombe SET1 ortholog suggest the existence of non-H3K4 Set1 targets relevant to gene regulation...
March 29, 2018: G3: Genes—Genomes—Genetics
https://www.readbyqxmd.com/read/29579541/set-promotes-h2ak9-acetylation-by-suppressing-hdac1-in-trichloroethylene-induced-hepatic-cytotoxicity
#17
Weixue Lu, Zhihong Chen, Xiaohu Ren, Wei Liu, Rongxia Deng, Jianhui Yuan, Xinfeng Huang, Weiguo Zhu, Jianjun Liu
Trichloroethylene (TCE) was widely used as an industrial solvent which could cause severe liver damage. The histone chaperone SET have been identified as an important mediator of TCE-induced hepatic cytotoxicity in our previous study; however, the underlying regulatory mechanisms remain poorly understood. In this study, we found a total of 136 histone acetylation sites involved in TCE-induced hepatic cytotoxicity with the technique of Triton-acid-urea polyacrylamide gel electrophoresis (TAU-PAGE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS)...
March 17, 2018: Environmental Toxicology and Pharmacology
https://www.readbyqxmd.com/read/29576542/histone-variant-h3-3-and-its-functions-in-reprogramming
#18
Xing-Wei Huang, Xiang-Rong Cheng, Nan Wang, Yu-Wei Zhang, Chen Liao, Lian-Hong Jin, Lei Lei
Histones are a class of evolutionarily conserved nuclear proteins. Histone octamer wrapped by DNA sequence forms the nucleosome, the basic building blocks of eukaryotic chromatin. The nucleosomes keep the DNA in a condensed state, maintain the integrity of the genome, and ensure proper DNA replication, transcription, recombination and repair. Nucleosomes can regulate the biological processes of the cell through a number of distinct post-translational modifications (PTMs) and turnovers of histone variants. Although the histone H3 variant H3...
March 20, 2018: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/29573427/transgenerational-phenotype-aggravation-in-caf-1-mutants-reveals-parent-of-origin-specific-epigenetic-inheritance
#19
Iva Mozgova, Thomas Wildhaber, Minerva S Trejo-Arellano, Jiri Fajkus, Pawel Roszak, Claudia Köhler, Lars Hennig
Chromatin is assembled by histone chaperones such as chromatin assembly factor CAF-1. We had noticed that vigor of Arabidopsis thaliana CAF-1 mutants decreased over several generations. Because changes in mutant phenotype severity over generations are unusual, we asked how repeated selfing of Arabidopsis CAF-1 mutants affects phenotype severity. CAF-1 mutant plants of various generations were grown, and developmental phenotypes, transcriptomes and DNA cytosine-methylation profiles were compared quantitatively...
March 24, 2018: New Phytologist
https://www.readbyqxmd.com/read/29545103/design-and-synthesis-of-triple-inhibitors-of-janus-kinase-jak-histone-deacetylase-hdac-and-heat-shock-protein-90-hsp90
#20
Lianbin Yao, Sten Ohlson, Brian W Dymock
Inhibition of multiple signaling pathways in a cancer cell with a single molecule could result in better therapies that are simpler to administer. Efficacy may be achieved with reduced potency against individual targets if there is synergy through multiple pathway inhibition. To achieve this, it is necessary to be able to build multi-component ligands by joining together key pharmacophores in a way which maintains sufficient activity against the individual pathways. In this work, designed triple inhibiting ligands are explored aiming to block three completely different target types: a kinase (JAK2), an epigenetic target (HDAC) and a chaperone (HSP90)...
May 1, 2018: Bioorganic & Medicinal Chemistry Letters
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