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https://www.readbyqxmd.com/read/29196644/adaptive-laboratory-evolution-of-corynebacterium-glutamicum-towards-higher-growth-rates-on-glucose-minimal-medium
#1
Eugen Pfeifer, Cornelia Gätgens, Tino Polen, Julia Frunzke
In this work, we performed a comparative adaptive laboratory evolution experiment of the important biotechnological platform strain Corynebacterium glutamicum ATCC 13032 and its prophage-free variant MB001 towards improved growth rates on glucose minimal medium. Both strains displayed a comparable adaptation behavior and no significant differences in genomic rearrangements and mutation frequencies. Remarkably, a significant fitness leap by about 20% was observed for both strains already after 100 generations...
December 1, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29194201/evaluation-of-long-term-anatomic-changes-following-canaloplasty-with-anterior-segment-spectral-domain-optical-coherence-tomography-and-ultrasound-biomicroscopy
#2
David Kuerten, Niklas Plange, Jakob Becker, Peter Walter, Matthias Fuest
BACKGROUND: To analyze long-term structural changes in conjunctiva, sclera and Schlemm canal (SC) with anterior segment optical coherence tomography (AS-OCT) and ultrasound biomicroscopy (UBM) following uncomplicated canaloplasty. MATERIALS AND METHODS: Fifteen patients with planned canaloplasty and no intraoperative complications were included in this prospective study. AS-OCT images were acquired at 1, 3, 6 months and at a long-term time point (20±4.9 mo) postsurgery...
November 30, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/29170973/primary-acute-angle-closure-glaucoma-complicating-ciliochoroidal-detachment-report-of-four-cases-and-review-of-the-literature
#3
Yan Zhang, Chao Wang, Li Liu, Yingqing Lei, Yue He, Ling Yu
PURPOSE: To describe the ultrasound biomicroscopy (UBM) and B-scan ultrasonography findings and therapeutic approach for ciliochoroidal detachment secondary to acute primary angle-closure glaucoma (APACG) in four patients. We also reviewed the literature. METHODS: Case report and systematic literature review. RESULTS: The four patients were referred to our department for further management of APACG. The UBM and B-scan ultrasonography examinations were conducted 2 days after the beginning of medical treatment and demonstrated ciliochoroidal detachment in four eyes of the four patients...
November 23, 2017: International Ophthalmology
https://www.readbyqxmd.com/read/29168133/assessment-of-oral-and-lung-bioaccessibility-of-cd-and-pb-from-smelter-impacted-dust
#4
Aurélie Pelfrêne, Francis Douay
Soil and dust contamination by metals engenders significant environmental and health problems in northern France where a lead smelter was in activity for more than a century. This study aims to examine the long-term effects of the smelter, 10 years after its closedown, on the presence of metal in sidewalk dust for a better assessment of the local population's exposure to Cd and Pb. The investigation included: (i) the metal distribution in different dust particle sizes and (ii) the assessment of metal bioaccessibility via ingestion and inhalation of dust...
November 22, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/29160547/ultrasound-biomicroscopy-in-acetazolamide-induced-myopic-shift-with-appositional-angle-closure
#5
Julio Daniel Grigera, Emilio Daniel Grigera
We report a case of a 49-year-old female who presented to the emergency department with blurred vision and vomiting, hours after taking two tablets of 250 mg of acetazolamide. The anterior chamber was bilaterally flat, with normal intraocular pressure in both eyes. An ultrasound biomicroscopic (UBM) examination showed bilateral ciliary effusion and complete appositional angle closure in all quadrants. Acetazolamide-induced bilateral angle closure was diagnosed. Steroid and cycloplegic therapy were initiated, and acetazolamide was discontinued...
September 2017: Arquivos Brasileiros de Oftalmologia
https://www.readbyqxmd.com/read/29156467/-diagnosis-and-therapy-of-iris-lesions
#6
Joel M Mor, Konrad R Koch, Ludwig M Heindl
The most common iris lesions are iris nevi, iris melanomas and iris pigment epithelium cysts. However, there is an abundance of rare differential diagnoses that have to be considered, including other melanocytic and non-melanocytic lesions. Diagnostic tools include the slit lamp examination, gonioscopy, tonometry, transillumination, ultrasound biomicroscopy (UBM), optical coherence tomography, fluorescein angiography and standardized photography-assisted documentation. The timely identification of malignant lesions (i...
November 20, 2017: Klinische Monatsblätter Für Augenheilkunde
https://www.readbyqxmd.com/read/29151682/anterior-segment-morphology-in-primary-angle-closure-glaucoma-using-ultrasound-biomicroscopy
#7
Tarannum Mansoori, Nagalla Balakrishna
Aim: To evaluate the configuration of the anterior chamber angle quantitatively and study the morphological changes in the eye with ultrasound biomicroscopy (UBM) in primary angle closure glaucoma (PACG) patients after laser peripheral iridotomy (LPI). Materials and methods: A total of 185 eyes of 185 PACG patients post-LPI and 126 eyes of 126 normal subjects were included in this prospective study. All subjects underwent complete ophthalmic evaluation, A-scan biometry, and UBM...
September 2017: Journal of Current Glaucoma Practice
https://www.readbyqxmd.com/read/29119030/latanoprost-induced-iris-pigment-epithelial-and-ciliary-body-cyst-formation-in-hypermetropic-eyes
#8
Abhijit Anand Mohite, Rangarajan V Prabhu, Thomas Ressiniotis
Purpose: Latanoprost has become one of the most widely prescribed topical antihypertensive medications in recent years. Yet there have been few reports of secondary iris pigment epithelial (IPE) and ciliary body (CB) cyst formation to date and none, to our knowledge, reported in eyes predisposed to primary angle closure. Methods: We report the first documented case of bilateral IPE and CB cysts in a hypermetropic patient with prior laser peripheral iridotomies (LPIs) as a rare, delayed side effect of topical Latanoprost treatment...
2017: Case Reports in Ophthalmological Medicine
https://www.readbyqxmd.com/read/29117006/phacoemulsification-in-eyes-with-post-trabeculectomy-choroidal-detachment
#9
Artemios S Kandarakis, George A Kontadakis, Stylianos A Kandarakis, Chrysostomos Dimitriou, Iordanis Chatziangelidis, Dimitrios Karagiannis
Four patients developed choroidal detachment within three months after trabeculectomy, documented with b-scan ultrasonography (BUS) and ultrasound biomicroscopy (UBM). Intraocular pressure (IOP) of the patients ranged from 3 to 5 mm Hg. Patients were treated with cycloplegia and steroids without complete resolution of detachment. Twelve months later all patients had developed visually significant cataracts and underwent phacoemulsification and intraocular lens implantation. Procedures were uncomplicated. Visual acuity ranged 0...
November 7, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/29111598/comparison-of-corneal-thickness-measurements-using-ultrasound-pachymetry-ultrasound-biomicroscopy-and-digital-caliper-in-frozen-canine-corneas
#10
Seowoo Jeong, Seonmi Kang, Sangwan Park, Eunjin Park, Jaegook Lim, Taekjin Nam, Kangmoon Seo
OBJECTIVE: To evaluate the clinical efficacy among ultrasound pachymetry (UP), ultrasound biomicroscopy (UBM), and manual measurement (MM) with digital caliper by comparing corneal thickness (COT) values obtained from frozen canine corneas ex vivo. PROCEDURES: COT was measured using UP, UBM, and MM in eight enucleated normal canine eyes frozen at -20 °C for 4 weeks. After thawing at room temperature for 2 h, the COT values were obtained from five sites on each cornea: central corneal thickness (CCOT), thickness at the 3, 6, 9, and 12 o'clock positions (3COT, 6COT, 9COT, and 12COT, respectively)...
November 7, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/29099668/successful-conservative-treatment-of-massive-choroidal-relapse-in-2-retinoblastoma-patients-monitored-by-ultrasound-biomicroscopy-and-or-spectral-domain-optic-coherence-tomography
#11
Christina Stathopoulos, Marie-Claire Gaillard, Francesco Puccinelli, Philippe Maeder, Doris Hadjistilianou, Maja Beck-Popovic, Francis L Munier
PURPOSE: To report the occurrence and management of secondary choroidal infiltration in two retinoblastoma (rb) patients. METHODS: Fundus examination and imaging with spectral domain optical coherence tomography (SD-OCT), B-scan ultrasonography (B-scan), and ultrasound biomicroscopy (UBM). RESULTS: Case 1: A 19-month-old girl with multifocal unilateral group B rb pretreated with intravenous chemotherapy (IVC) was referred for further management...
November 3, 2017: Ophthalmic Genetics
https://www.readbyqxmd.com/read/29077625/chronic-and-intermittent-angle-closure-caused-by-in-the-bag-capsular-tension-ring-and-intraocular-lens-dislocation-in-patients-with-pseudoexfoliation-syndrome
#12
Frank Bochmann, Jörg Strümer
PURPOSE: A subluxation or dislocation of the intraocular lens (IOL) is a well-known complication after cataract surgery in patients with pseudoexfoliation syndrome (PEX). Capsular tension rings (CTR) are frequently used to prevent postoperative complications caused by zonular weakness. Here we present a series of 7 cases, 2 with intermittent and 5 with acute angle closure caused by a combination of PEX-associated zonular weakness and a resulting unstable capsular bag-CTR-IOL complex. MATERIALS AND METHODS: We describe the typical clinical course with elevated intraocular pressure, myopic shift, and shallowing of the anterior chamber in 7 patients with a new type of secondary angle closure...
November 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/29044057/clinicodemographic-profile-and-treatment-outcome-in-patients-of-ocular-surface-squamous-neoplasia
#13
Rachna Meel, Rebika Dhiman, Murugesan Vanathi, Neelam Pushker, Radhika Tandon, Saranya Devi
PURPOSE: The aim is to study the clinicodemographic profile and treatment outcome of ocular surface squamous neoplasia (OSSN). METHODS: This was a retrospective observational study of 57 eyes (56 cases) with clinically diagnosed OSSN, presenting in our center over the past year. RESULTS: The median age of presentation was 55 years with male:female ratio being 4.5:1. Systemic predisposing conditions were xeroderma pigmentosa (1) postkidney transplant immunosuppression (1), and human immunodeficiency virus infection (1)...
October 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/29040321/co-delivery-of-micronized-urinary-bladder-matrix-damps-regenerative-capacity-of-minced-muscle-grafts-in-the-treatment-of-volumetric-muscle-loss-injuries
#14
Stephen M Goldman, Benjamin T Corona
Minced muscle grafts (MG) promote de novo muscle fiber regeneration and neuromuscular strength recovery in small and large animal models of volumetric muscle loss. The most noteworthy limitation of this approach is its reliance on a finite supply of donor tissue. To address this shortcoming, this study sought to evaluate micronized acellular urinary bladder matrix (UBM) as a scaffolding to promote in vivo expansion of this MG therapy in a rat model. Rats received volumetric muscle loss injuries to the tibialis anterior muscle of their left hind limb which were either left untreated or repaired with minced muscle graft at dosages of 50% and 100% of the defect mass, urinary bladder matrix in isolation, or a with an expansion product consisting of a combination of the two putative therapies in which the minced graft is delivered at a dosage of 50% of the defect mass...
2017: PloS One
https://www.readbyqxmd.com/read/29037659/the-accuracy-of-wide-field-ultrasound-biomicroscopy-in-localizing-extraocular-rectus-muscle-insertions-in-strabismus-reoperations
#15
Arash Mirmohammadsadeghi, Vahideh Manuchehri, Mohammad Reza Akbari
PURPOSE: To compare the accuracy of widefield ultrasound biomicroscopy (UBM) with mechanical intraoperative measurements of the distance between rectus muscle insertions and the corneal limbus in strabismus reoperations. METHODS: Subjects with a history of horizontal rectus muscle surgery who required further surgery on horizontal rectus muscle(s) were recruited prospectively. All widefield UBM measurements were carried out under topical anesthesia using a 50 MHz linear probe without immersion cup and external caliper...
December 2017: Journal of AAPOS: the Official Publication of the American Association for Pediatric Ophthalmology and Strabismus
https://www.readbyqxmd.com/read/29034562/canine-central-corneal-thickness-measurements-via-pentacam-hr-%C3%A2-optical-coherence-tomography-optovue-ivue-%C3%A2-and-high-resolution-ultrasound-biomicroscopy
#16
Allison E Wolfel, Samantha L Pederson, Allison M Cleymaet, Ann M Hess, Kate S Freeman
OBJECTIVE: To compare central corneal thickness (CCT) values in canine eyes using Pentacam-HR(®) Scheimpflug imaging (Pentacam), Optovue(®) iVue spectral-domain optical coherence tomography (SD-OCT), and high-resolution ultrasound biomicroscopy (UBM) and generate normative canine Pentacam CCT values. ANIMALS STUDIED: Twenty-four client-owned dogs (37 eyes) with nondiseased cornea(s) presenting to the Colorado State University Veterinary Teaching Hospital. PROCEDURES: Corneal images were acquired via Pentacam, SD-OCT, and UBM in the listed order...
October 15, 2017: Veterinary Ophthalmology
https://www.readbyqxmd.com/read/29028106/correlation-between-presence-of-primary-iris-and-cilliary-body-cysts-and-intraocular-pressure
#17
K-C Xue, D-D Hu, L Zhao, N Li, H-Y Shen
OBJECTIVE: The objective of this study was to examine the correlation between the presence of primary iris-ciliary cysts and the intraocular pressure. PATIENTS AND METHODS: Sixty patients with short-sightedness undergoing routine examination for laser vision correction in our hospital in 2003 were enrolled. Patients with known high intraocular pressure and risk of glaucoma were excluded from the study. A total of 119 eyes were examined by the Ultrasound Biomicroscope (UBM), and the presence of the primary iris-ciliary cysts was confirmed...
September 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28991616/refractive-lens-exchange-and-piggyback-intraocular-lens-implantation-in-nanophthalmos-visual-and-structural-outcomes
#18
Masoomeh Mohebbi, Mohammad-Reza Fallah-Tafti, Kaveh Fadakar, Ramon Katoozpour, Seyed-Farzad Mohammadi, Zahra Fallah-Tafti, Azita Khorami
PURPOSE: To evaluate the best piggyback intraocular lens (IOL) implantation method and the outcomes in nanophthalmos eyes and to define the postoperative structural changes. SETTING: Farabi Eye Hospital, Tehran, Iran. DESIGN: Prospective case series. METHODS: Candidates for refractive surgery who were nanophthalmic were recruited. Patients had refractive lens exchange followed by implantation of both IOLs in the bag or 1 IOL in the bag and 1 IOL in the sulcus...
September 2017: Journal of Cataract and Refractive Surgery
https://www.readbyqxmd.com/read/28986594/plateau-iris-distribution-across-anterior-segment-optical-coherence-tomography-defined-subgroups-of-subjects-with-primary-angle-closure-glaucoma
#19
Sushma Verma, Monisha E Nongpiur, Hnin H Oo, Eray Atalay, David Goh, Tina T Wong, Shamira A Perera, Tin Aung
Purpose: We previously identified three distinct subgroups of patients with primary angle closure glaucoma (PACG) based on anterior segment optical coherence tomography (ASOCT) imaging. Group 1 was characterized by a large iris area with deepest anterior chambers, group 2 by a large lens vault (LV) and shallow anterior chamber depth (ACD), and group 3 displayed intermediate values across iris area, LV, and ACD. The purpose of the present study was to determine the distribution of plateau iris in these subgroups using ultrasound biomicroscopy (UBM) features...
October 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28956856/towards-a-continuous-biometric-system-based-on-ecg-signals-acquired-on-the-steering-wheel
#20
João Ribeiro Pinto, Jaime S Cardoso, André Lourenço, Carlos Carreiras
Electrocardiogram signals acquired through a steering wheel could be the key to seamless, highly comfortable, and continuous human recognition in driving settings. This paper focuses on the enhancement of the unprecedented lesser quality of such signals, through the combination of Savitzky-Golay and moving average filters, followed by outlier detection and removal based on normalised cross-correlation and clustering, which was able to render ensemble heartbeats of significantly higher quality. Discrete Cosine Transform (DCT) and Haar transform features were extracted and fed to decision methods based on Support Vector Machines (SVM), k-Nearest Neighbours (kNN), Multilayer Perceptrons (MLP), and Gaussian Mixture Models - Universal Background Models (GMM-UBM) classifiers, for both identification and authentication tasks...
September 28, 2017: Sensors
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