keyword
MENU ▼
Read by QxMD icon Read
search

Carbapenemase

keyword
https://www.readbyqxmd.com/read/28337342/first-indian-report-of-incx3-plasmid-carrying-blandm-7-in-escherichia-coli-from-bloodstream-infection-potential-for-rapid-dissemination
#1
N K Devanga Ragupathi, D P Muthuirulandi Sethuvel, R Gajendiran, J L K Daniel, K Walia, B Veeraraghavan
Enterobacteriaceae with blaNDM-7 is only infrequently observed. Self-transmissible plasmids carrying the blaNDM gene increase the dissemination of carbapenem resistance in developing countries. This study investigates the whole genome sequence of a blaNDM-7-positive Escherichia coli. The isolate was an extended-spectrum β-lactamase producer by combined disc diffusion test and carbapenemase producer by CarbaNP method. Sequencing results revealed the isolate as E. coli ST-167 with IncX3 plasmid carrying blaNDM-7 in addition to blaTEM-1 and blaCMY-42 genes...
May 2017: New Microbes and New Infections
https://www.readbyqxmd.com/read/28337042/household-transmission-of-ndm-producing-e-coli-in-new-zealand
#2
Matthew Blakiston, Sally A Roberts, Joshua T Freeman, Helen Heffernan
This report describes the introduction of an extensively antibiotic-resistant carbapenemase-producing Escherichia coli into a hospital in Auckland, New Zealand, by a patient who was a household contact of recent travellers to the Indian subcontinent. The carbapenemase was identified as New Delhi metallo-β-lactamase (NDM) and reflects probable household transmission in the context of a recent upsurge in NDM-producing Enterobacteriaceae isolation in New Zealand. The observations in this report suggest that hospital screening practices to identify carbapenemase-producing Enterobacteriaceae (CPE) colonised patients may need to be extended to include travellers to high-risk countries who were not hospitalised during their trip, and possibly also their close contacts...
March 24, 2017: New Zealand Medical Journal
https://www.readbyqxmd.com/read/28333363/evaluation-of-the-%C3%AE-carba%C3%A2-test-a-colorimetric-test-for-the-rapid-detection-of-carbapenemase-activity-in-gram-negative-bacilli
#3
Sandrine Bernabeu, Laurent Dortet, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated a novel colorimetric test (the β-CARBA™ test; Bio-Rad) to detect carbapenemase-producing Gram-negative bacilli from cultured colonies. Methods: The performance of the β-CARBA™ test was compared with that of the Carba NP test (or the CarbAcineto NP test) and RAPIDEC ® CARBA NP (bioMérieux) using a collection of 290 isolates with characterized β-lactamase content...
March 10, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333331/multiregional-dissemination-of-kpc-producing-klebsiella-pneumoniae-st258-st512-genotypes-in-poland-2010-14
#4
Anna Baraniak, Radoslaw Izdebski, Dorota Zabicka, Katarzyna Bojarska, Sandra Górska, Elzbieta Literacka, Janusz Fiett, Waleria Hryniewicz, Marek Gniadkowski
Objectives: In 2008-09, the KPC carbapenemase epidemiology in Poland was dominated by a Klebsiella pneumoniae ST258 KPC-2 outbreak in Warsaw and its administrative region. The aim of this study was to analyse the situation in 2010-14, with a focus on new outbreaks in other parts of the country. Methods: KPCs were detected in all suspected isolates by PCR. The detailed study was performed on 173 isolates from 2010 to 2012, and included PFGE and MLST, PCR identification of K...
March 9, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333319/wck-4234-a-novel-diazabicyclooctane-potentiating-carbapenems-against-enterobacteriaceae-pseudomonas-and-acinetobacter-with-class-a-c-and-d-%C3%AE-lactamases
#5
Shazad Mushtaq, Anna Vickers, Neil Woodford, David M Livermore
Background: Several diazabicyclooctanes (DBOs) are under development as inhibitors of class A and C β-lactamases. Inhibition of OXA (class D) carbapenemases is variable, with those of Acinetobacter spp. remaining notably resistant. We describe a novel DBO, WCK 4234 (Wockhardt), with distinctive activity against OXA carbapenemases. Methods: MICs of imipenem and meropenem were determined by CLSI agar dilution with WCK 4234 added at 4 or 8 mg/L. Test organisms were clinical Enterobacteriaceae, Acinetobacter baumannii and Pseudomonas aeruginosa with carbapenemases or carbapenem resistance via porin loss plus AmpC or ESBL activity...
February 16, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28333193/increased-activity-of-colistin-in-combination-with-amikacin-against-escherichia-coli-co-producing-ndm-5-and-mcr-1
#6
Yu-Feng Zhou, Meng-Ting Tao, Youjun Feng, Run-Shi Yang, Xiao-Ping Liao, Ya-Hong Liu, Jian Sun
Objectives: Colistin and carbapenem are two lines of last-resort antibiotics against lethal infections caused by MDR Gram-negative pathogens. The emergence of carbapenemase-positive Escherichia coli with colistin resistance poses a serious threat to public health worldwide. Here we report, for the first time (to the best of our knowledge), a novel combination therapy used for the treatment of E. coli co-producing MCR-1 and NDM-5. Methods: The MICs of colistin were determined alone and with 1-4 mg/L amikacin...
February 20, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28332754/detection-of-carbapenemase-producing-organisms-with-a-carbapenem-based-fluorogenic-probe
#7
Wuyu Mao, Lingying Xia, Hexin Xie
Antibiotic resistance has become a major challenge to public health worldwide. This crisis is further aggravated by the increasing emergence of bacterial resistance to carbapenems, typically considered as the antibiotics of last resort, which is mainly due to the production of carbapenem-hydrolyzing carbapenemases in bacteria. Herein, the carbapenem-based fluorogenic probe CB-1 with an unprecedented enamine-BODIPY switch is developed for the detection of carbapenemase activity. This reagent is remarkably specific towards carbapenemases over other prevalent β-lactamases...
March 23, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28320716/in-vitro-activity-of-imipenem-relebactam-against-gram-negative-eskape-pathogens-isolated-by-clinical-laboratories-in-the-united-states-in-2015-results-from-the-smart-global-surveillance-program
#8
Sibylle H Lob, Meredith A Hackel, Krystyna M Kazmierczak, Katherine Young, Mary R Motyl, James A Karlowsky, Daniel F Sahm
Relebactam (formerly MK-7655) is an inhibitor of class A and C β-lactamases, including Klebsiella pneumoniae carbapenemase (KPC), and is currently in clinical development in combination with imipenem/cilastatin. Using Clinical and Laboratory Standards Institute (CLSI) defined broth microdilution methodology, we evaluated the in vitro activities of imipenem-relebactam, imipenem, and seven routinely tested parenteral antimicrobial agents against gram-negative ESKAPE pathogens (Pseudomonas aeruginosa, n = 845; Klebsiella pneumoniae, n = 689; Enterobacter spp...
March 20, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28320396/occurrence-of-bla-dha-1-mediated-cephalosporin-resistance-in-escherichia-coli-and-their-transcriptional-response-against-cephalosporin-stress-a-report-from-india
#9
Birson Ingti, Deepjyoti Paul, Anand Prakash Maurya, Debajyoti Bora, Debadatta Dhar Chanda, Atanu Chakravarty, Amitabha Bhattacharjee
BACKGROUND: Treatment alternatives for DHA-1 harboring strains are challenging as it confers resistance to broad spectrum cephalosporins and may further limit treatment option when expressed at higher levels. Therefore, this study was designed to know the prevalence of DHA genes and analyse the transcription level of DHA-1 against different β-lactam stress. METHODS: Screening of AmpC β-lactamase phenotypically by modified three dimensional extract method followed by Antimicrobial Susceptibility and MIC determination...
March 21, 2017: Annals of Clinical Microbiology and Antimicrobials
https://www.readbyqxmd.com/read/28303836/carbapenem-nonsusceptibility-with-modified-oprd-in-clinical-isolates-of-pseudomonas-aeruginosa-from-india
#10
Debarati Choudhury, Anupam Das Talukdar, Manabendra Dutta Choudhury, Anand Prakash Maurya, Debadatta Dhar Chanda, Atanu Chakravorty, Amitabha Bhattacharjee
This study was undertaken to investigate OprD porin-mediated carbapenem nonsusceptibility in clinical isolates of Pseudomonas aeruginosa from a tertiary referral hospital of Northeast India. A total of 267 nonduplicate, consecutive clinical isolates of P. aeruginosa were obtained. Mutation and expression levels of OprD gene were determined in carbapenem-nonsusceptible carbapenemase-nonproducing isolates. Among 19 carbapenem-nonsusceptible carbapenemase-nonproducing isolates, 11 of them demonstrated variable band pattern while performing denaturing gradient gel electrophoresis with amplified products of OprD gene...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303831/strengths-and-limitations-of-various-screening-methods-for-carbapenem-resistant-enterobacteriaceae-including-new-method-recommended-by-clinical-and-laboratory-standards-institute-2017-a-tertiary-care-experience
#11
Agila Kumari Pragasam, Balaji Veeraraghavan, Yamuna Devi Bakthavatchalam, Radha Gopi, Raziya Fathima Aslam
Carbapenemase-mediated carbapenem resistance is a major concern across the world. Rapid detection of carbapenemase-producing organisms is of great importance in clinical settings. However, it is essential to have a test with good sensitivity and specificity. The aim of the study was to compare the performance of RAPIDEC® CARBA NP and modified carbapenem inactivation method (mCIM) recommended by Clinical and Laboratory Standards Institute guideline 2017. A total of ninety carbapenem resistant Escherichia coli and Klebsiella pneumoniae have been tested...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28303191/vim-1-carbapenemase-producing-escherichia-coli-in-gulls-from-southern-france
#12
Marion Vittecoq, Chrislène Laurens, Lionel Brazier, Patrick Durand, Eric Elguero, Audrey Arnal, Frédéric Thomas, Salim Aberkane, Nicolas Renaud, Franck Prugnolle, Jérôme Solassol, Hélène Jean-Pierre, Sylvain Godreuil, François Renaud
Acquired carbapenemases currently pose one of the most worrying public health threats related to antimicrobial resistance. A NDM-1-producing Salmonella Corvallis was reported in 2013 in a wild raptor. Further research was needed to understand the role of wild birds in the transmission of bacteria resistant to carbapenems. Our aim was to investigate the presence of carbapenem-resistant Escherichia coli in gulls from southern France. In 2012, we collected 158 cloacal swabs samples from two gull species: yellow-legged gulls (Larus michahellis) that live in close contact with humans and slender-billed gulls (Chroicocephalus genei) that feed at sea...
February 2017: Ecology and Evolution
https://www.readbyqxmd.com/read/28302074/clonality-outer-membrane-proteins-profile-and-efflux-pump-in-kpc-producing-enterobacter-sp-in-brazil
#13
Juliana Ferraz Rosa, Camila Rizek, Ana Paula Marchi, Thais Guimaraes, Lourdes Miranda, Claudia Carrilho, Anna S Levin, Silvia F Costa
BACKGROUND: Carbapenems resistance in Enterobacter spp. has increased in the last decade, few studies, however, described the mechanisms of resistance in this bacterium. This study evaluated clonality and mechanisms of carbapenems resistance in clinical isolates of Enterobacter spp. identified in three hospitals in Brazil (Hospital A, B and C) over 7-year. METHODS: Antibiotics sensitivity, pulsed-field gel electrophoresis (PFGE), PCR for carbapenemase and efflux pump genes were performed for all carbapenems-resistant isolates...
March 17, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28298454/in-vitro-activity-of-imipenem-against-carbapenemase-positive-enterobacteriaceae-smart-global-surveillance-program-2008-2014
#14
James A Karlowsky, Sibylle H Lob, Krystyna M Kazmierczak, Robert E Badal, Katherine Young, Mary R Motyl, Daniel F Sahm
The Study for Monitoring Antimicrobial Resistance Trends (SMART) global surveillance program collected 103,960 isolates of Enterobacteriaceae from 2008 to 2014. From this isolate collection all ertapenem-non-susceptible isolates (MIC, ≥1 μg/ml; n = 3,428) and 9,371 isolates of Escherichia coli, Klebsiella pneumoniae, Klebsiella oxytoca, and Proteus mirabilis with an ertapenem-susceptible extended-spectrum β-lactamase (ESBL)-positive phenotype were assessed for the presence of common carbapenemase genes using the Check-MDR CT101 microarray (Check-Points, Wageningen, the Netherlands) and published multiplex PCR assays...
March 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28295617/carbapenemases-producing-klebsiella-pneumoniae-in-hospitals-of-two-regions-of-southern-italy
#15
Carla Calia, Carlo Pazzani, Marta Oliva, Maria Scrascia, Piero Lovreglio, Carmen Capolongo, Anna Maria Dionisi, Adriana Chiarelli, Rosa Monno
Carbapenem-resistant Klebsiella pneumoniae infections are reported with increasing frequency elsewhere in the world, representing a worrying phenomenon for global health. In Italy, there are hotspot data on the diffusion and type of carbapenemase-producing Enterobacteriaceae and K. pneumoniae in particular, with very few data coming from Apulia and Basilicata, two regions of Southern Italy. This study was aimed at characterizing by phenotypic and genotypic methods carbapenem-resistant K. pneumoniae isolated from several Hospitals of Apulia and Basilicata, Southern Italy...
March 15, 2017: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
https://www.readbyqxmd.com/read/28293307/sensitivity-to-antimicrobial-drugs-of-pseudomonas-aeruginosa-extreme-resistant-strains-isolated-in-the-major-hospitals-of-central-kazakhstan
#16
Ilya S Azizov, Alyona V Lavrinenko, Ilya A Belyaev, Dmitry B Babenko, Natalya A Shambilova, Nelya M Bissenova
AIM: The article presents the current data on the sensitivity of the main 37 strains of eXtremaly Drugs Resistance (XDR) category to anti-pseudomonas drugs. MATERIAL AND METHODS: The strains were collected during the prospective multicenter study in large multidisciplinary hospitals of Central Kazakhstan. Susceptibility to antimicrobial drugs was carried out by disk method and the serial dilution method with the interpretation of the results according to EUCAST criteria...
March 15, 2017: Open Access Macedonian Journal of Medical Sciences
https://www.readbyqxmd.com/read/28291698/detection-and-analysis-of-different-interactions-between-resistance-mechanisms-and-carbapenems-in-clinical-isolates-of-klebsiella-pneumoniae
#17
Tanise Vendruscolo Dalmolin, Bianca Vendruscolo Bianchini, Grazielle Guidolin Rossi, Ana Carolina Ramos, Ana Cristina Gales, Priscila de Arruda Trindade, Marli Matiko Anraku de Campos
Carbapenems are considered last-line agents for the treatment of serious infections caused by Klebsiella pneumoniae, and this microorganism may exhibit resistance to β-lactam antibiotics due to different mechanisms of resistance. We evaluated 27 isolates of K. pneumoniae resistant to carbapenems recovered from inpatients at the University Hospital of Santa Maria-RS from July 2013 to August 2014. We carried out antimicrobial susceptibility, carbapenemase detection, testing for the presence of efflux pump by broth microdilution and loss of porin by sodium dodecyl sulfate polyacrylamide gel electrophoresis...
February 9, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28291671/molecular-characterization-of-kpc-producing-klebsiella-pneumoniae-isolated-from-patients-in-a-public-hospital-in-caracas-venezuela
#18
Aura Dayana Falco Restrepo, Mariel Alexandra Velásquez Nieves, Howard Takiff
INTRODUCTION: Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria are amongst the most important causative agents of nosocomial infections worldwide. Isolates of this bacterium have been identified in Venezuela but little is known about their local spread. The aim of this study was to perform the molecular characterization of KPC-producing strains isolated from 2012 to 2013 in a public hospital in Caracas, Venezuela. METHODS: Twenty-two K. pneumoniae clinical isolates phenotypically classified as KPC producing were subjected to PCR screening for the presence of blaKPC genes and their location within transposon Tn4401...
March 11, 2017: Enfermedades Infecciosas y Microbiología Clínica
https://www.readbyqxmd.com/read/28291628/invitro-activity-of-imipenem-relebactam-against-gram-negative-bacilli-isolated-from-patients-with-lower-respiratory-tract-infections-in-the-united-states-in-2015-results-from-the-smart-global-surveillance-program
#19
Sibylle H Lob, Meredith A Hackel, Krystyna M Kazmierczak, Daryl J Hoban, Katherine Young, Mary R Motyl, James A Karlowsky, Daniel F Sahm
The β-lactamase inhibitor relebactam inactivates class A β-lactamases, including KPC-type carbapenemases, and class C β-lactamases. Relebactam combined with imipenem is in clinical development for several indications, including hospital-acquired and ventilator-associated pneumonia. Employing CLSI-defined broth microdilution methodology, we evaluated the activities of imipenem-relebactam (using imipenem MIC breakpoints) and comparators against non-Proteeae Enterobacteriaceae (n=853) and Pseudomonas aeruginosa (n=598) isolated from lower respiratory tract infection samples in 20 hospital laboratories in the United States participating in the 2015 SMART (Study for Monitoring Antimicrobial Resistance Trends) global surveillance program...
March 2, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28288398/characterization-of-extended-spectrum-%C3%AE-lactamases-esbls-producing-salmonella-in-retail-raw-chicken-carcasses
#20
Jing Qiao, Qiang Zhang, Walid Q Alali, Jiawei Wang, Lingyuan Meng, Yingping Xiao, Hua Yang, Sheng Chen, Shenghui Cui, Baowei Yang
Extended-spectrum β-lactamases (ESBLs)-producing Salmonella is considered a serious concern to public health worldwide. However, limited information is available on ESBLs-producing Salmonella in retail chicken products in China. The objective of this study was to characterize ESBLs-producing Salmonella isolates from retail chickens in China. A total of 890 Salmonella isolates from retail chicken carcasses collected from 4 provinces were firstly screened for ESBLs-production phenotype via the double-disk synergy test method...
February 24, 2017: International Journal of Food Microbiology
keyword
keyword
190
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"