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Plakin antibodies

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https://www.readbyqxmd.com/read/27087170/longitudinal-analysis-of-antibody-profiles-against-plakins-in-severe-drug-eruptions-emphasis-on-correlation-with-tissue-damage-in-dihs-dress
#1
A Takehara, Y Aoyama, M Kurosawa, Y Shirafuji, H Umemura, K Kamiya, Y Ushigome, Y Kano, T Shiohara, K Iwatsuki
BACKGROUND: The evidence for severe drug eruption as a trigger for autoimmune disease has recently increased. No information is available on how tissue damage in severe drug eruptions can induce autoimmune responses. OBJECTIVES: To investigate whether the generation of autoantibodies (autoAbs) against plakin family proteins could be the cause or result of tissue damage in patients with severe drug eruptions and whether the generation of autoAbs could be prevented by systemic corticosteroids during the acute stage...
April 18, 2016: British Journal of Dermatology
https://www.readbyqxmd.com/read/26909655/anti-early-endosome-antigen-1-autoantibodies-were-detected-in-a-pemphigus-like-patient-but-not-in-the-majority-of-pemphigus-diseases
#2
Ryuhei Nishikawa, Hitoshi Takahashi, Mitsuhiro Matsuda, Kaoru Imaoka, Masahiro Ogawa, Kwesi Teye, Atsunari Tsuchisaka, Hiroshi Koga, Lars Komorowski, Christian Probst, Takahisa Hachiya, Marvin J Fritzler, Norito Ishii, Chika Ohata, Minao Furumura, Rafal P Krol, Yoshinao Muro, Eishin Morita, Takashi Hashimoto
Although the major autoantigens in classic pemphigus are desmogleins, sera from various types of pemphigus react with a number of other molecules, including desmocollins and plakin proteins. However, other novel pemphigus-related autoantigens remain to be identified. In this study, immunoblotting for serum from an atypical autoimmune bullous disease patient identified an unknown 175 kDa protein. Subsequent studies using two-dimensional gel electrophoresis, immunoblotting and mass-spectrometry identified the 175 kDa protein as early endosome antigen 1 (EEA1)...
May 2016: Experimental Dermatology
https://www.readbyqxmd.com/read/26802236/epiplakin-is-a-paraneoplastic-pemphigus-autoantigen-and-related-to-bronchiolitis-obliterans-in-japanese-patients
#3
COMPARATIVE STUDY
Atsunari Tsuchisaka, Sanae Numata, Kwesi Teye, Yohei Natsuaki, Tamihiro Kawakami, Yoshito Takeda, Wenqing Wang, Kazushi Ishikawa, Mizuki Goto, Hiroshi Koga, Ryosuke Sogame, Norito Ishii, Shinzo Takamori, Tomoaki Hoshino, Oliver Brandt, Hendri H Pas, Sakuhei Fujiwara, Takashi Hashimoto
All plakin family proteins are known to be autoantigens in paraneoplastic pemphigus (PNP). In this study, we first examined whether PNP sera also react with epiplakin, another plakin protein, by various immunological methods using 48 Japanese PNP sera. Immunofluorescence confirmed that cultured keratinocytes expressed epiplakin. Epiplakin was detected by 72.9% of PNP sera by immunoprecipitation-immunoblotting with KU-8 cell extract, but not by immunoblotting of either normal human epidermal extract or KU-8 cell extract...
February 2016: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/26480281/epiplakin-is-a-paraneoplastic-pemphigus-autoantigen-and-related-to-bronchiolitis-obliterans-in-japanese-patients
#4
Atsunari Tsuchisaka, Sanae Numata, Kwesi Teye, Yohei Natsuaki, Tamihiro Kawakami, Yoshito Takeda, Wenqing Wang, Kazushi Ishikawa, Mizuki Goto, Hiroshi Koga, Ryosuke Sogame, Norito Ishii, Shinzo Takamori, Tomoaki Hoshino, Oliver Brandt, Hendri H Pas, Sakuhei Fujiwara, Takashi Hashimoto
All plakin family proteins are known to be autoantigens in paraneoplastic pemphigus (PNP). In this study, we first examined whether PNP sera also react with epiplakin, another plakin protein, by various immunological methods using 48 Japanese PNP sera. Immunofluorescence confirmed that cultured keratinocytes expressed epiplakin. Epiplakin was detected by 72.9% of PNP sera by immunoprecipitation-immunoblotting with KU-8 cell extract, but not by immunoblotting of either normal human epidermal extract or KU-8 cell extract...
October 19, 2015: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/25797172/ige-autoantibodies-in-bullous-pemphigoid-supporting-role-or-leading-player
#5
REVIEW
Hideyuki Ujiie
Bullous pemphigoid (BP) is a common autoimmune blistering skin disease in which two hemidesmosomal components--the transmembrane collagen XVII (BP180 or BPAG2) and the plakin family protein BP230 (BPAG1)--are targeted by autoimmunity. Of these, collagen XVII (COL17) is thought to be a major autoantigen, and vital roles of IgG autoantibodies in blister formation have been elucidated. However, BP shows distinct features, including pruritic urticarial erythema and eosinophilic infiltration, which may be independent of IgG-mediated autoimmunity...
April 2015: Journal of Dermatological Science
https://www.readbyqxmd.com/read/24557264/utility-of-serological-assays-in-the-diagnosis-of-paraneoplastic-pemphigus
#6
Shane Kelly, Suzanne Culican, Roger Silvestrini, Mark Schifter, David A Fulcher, Ming-Wei Lin
BACKGROUND: Paraneoplastic pemphigus (PNP) is a rare autoimmune mucocutaneous blistering condition which has a universal association with malignancy and a poor prognosis. PNP, which is characterised by the presence of autoantibodies against plakins in the skin epithelial layer, can be a challenging diagnosis to make, particularly in those without an existing history of malignancy, as tissue biopsy findings may resemble other disorders that have similar clinical features. Thus, serology may be helpful in the diagnosis...
February 2014: Pathology
https://www.readbyqxmd.com/read/24457756/utility-of-serological-assays-in-the-diagnosis-of-paraneoplastic-pemphigus
#7
Shane Kelly, Suzanne Culican, Roger Silvestrini, Mark Schifter, David A Fulcher, Ming-Wei Lin
BACKGROUND: Paraneoplastic pemphigus (PNP) is a rare autoimmune mucocutaneous blistering condition which has a universal association with malignancy and a poor prognosis. PNP, which is characterised by the presence of autoantibodies against plakins in the skin epithelial layer, can be a challenging diagnosis to make, particularly in those without an existing history of malignancy, as tissue biopsy findings may resemble other disorders that have similar clinical features. Thus, serology may be helpful in the diagnosis...
February 2014: Pathology
https://www.readbyqxmd.com/read/24275244/detection-of-autoantibodies-to-periplakin-and-envoplakin-in-paraneoplastic-pemphigus-but-not-idiopathic-pulmonary-fibrosis-using-full-length-recombinant-proteins
#8
Yoshinao Muro, Kazumitsu Sugiura, Akira Shiraki, Norito Ishii, Takashi Hashimoto, Masashi Akiyama
BACKGROUND: Paraneoplastic pemphigus (PNP) serum preferentially reacts with periplakin and envoplakin, which are plakin family proteins localized to desmosomes and intermediate filaments. Recently, anti-periplakin antibodies were also detected in patients with idiopathic pulmonary fibrosis (IPF). Although previous epitope-mapping studies showed multiple epitopes in each protein, enzyme-linked immunosorbent assays have used several truncated, but not full-length, recombinant proteins. METHODS: This study aimed to produce full-length biotinylated recombinant proteins of periplakin and envoplakin for detection of autoantibodies by immunoprecipitation and ELISA...
February 15, 2014: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/23796242/laboratory-diagnosis-of-paraneoplastic-pemphigus
#9
A M Poot, G F H Diercks, D Kramer, I Schepens, G Klunder, T Hashimoto, L Borradori, M F Jonkman, H H Pas
BACKGROUND: Paraneoplastic pemphigus (PNP) is a multiorgan disease characterized by antibodies against plakins, desmogleins and the α2-macroglobulin-like-1 (A2ML1) protein, in association with an underlying neoplasm. Accurate diagnosis relies on the demonstration of these autoantibodies in serum. OBJECTIVES: To evaluate the value of different laboratory techniques in the serological diagnosis of PNP. METHODS: We performed immunoblotting, envoplakin (EP) enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence (IIF) on rat bladder, radioactive immunoprecipitation and a nonradioactive combined immunoprecipitation-immunoblot assay...
November 2013: British Journal of Dermatology
https://www.readbyqxmd.com/read/23407400/anti-%C3%AE-2-macroglobulin-like-1-autoantibodies-are-detected-frequently-and-may-be-pathogenic-in-paraneoplastic-pemphigus
#10
COMPARATIVE STUDY
Sanae Numata, Kwesi Teye, Daisuke Tsuruta, Ryosuke Sogame, Norito Ishii, Hiroshi Koga, Yohei Natsuaki, Atsunari Tsuchisaka, Takahiro Hamada, Tadashi Karashima, Takekuni Nakama, Minao Furumura, Chika Ohata, Tamihiro Kawakami, Isabelle Schepens, Luca Borradori, Takashi Hashimoto
Paraneoplastic pemphigus (PNP) shows autoantibodies mainly to plakin and desmosomal cadherin family proteins. We have recently identified alpha-2-macroglobulin-like-1 (A2ML1), a broad range protease inhibitor, as a unique PNP antigen. In this study, we tested a large number of PNP sera by various methods. Forty (69.0%) of 58 PNP sera recognized A2ML1 recombinant protein expressed in COS7 cells by immunofluorescence (IF) and/or immunoprecipitation (IP)/immunoblotting (IB). IP/IB showed higher sensitivity than IF...
July 2013: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/22801794/prognostic-factors-of-paraneoplastic-pemphigus
#11
MULTICENTER STUDY
Sandy Leger, Damien Picard, Saskia Ingen-Housz-Oro, Jean-Philippe Arnault, François Aubin, Francis Carsuzaa, Gilles Chaumentin, Jacqueline Chevrant-Breton, Olivier Chosidow, Béatrice Crickx, Michel D'incan, Michel Dandurand, Stéphane Debarbieux, Emmanuel Delaporte, Olivier Dereure, Marie-Sylvie Doutre, Gérard Guillet, Denis Jullien, Ingrid Kupfer, Jean-Philippe Lacour, Fabienne Leonard, Catherine Lok, Laurent Machet, Ludovic Martin, Carle Paul, Jean-Michel Pignon, Caroline Robert, Luc Thomas, Pierre-Jean Weiller, Vincent Ferranti, Danièle Gilbert, Philippe Courville, Estelle Houivet, Jacques Benichou, Pascal Joly
OBJECTIVE: To identify the prognostic factors of overall survival in a series of patients with paraneoplastic pemphigus (PNP). DESIGN: Multicenter retrospective cohort study. SETTING: Twenty-seven dermatology departments in France. PATIENTS: A total of 53 patients (31 men and 22 women; median age, 59 years; age range, 30-88 years) were diagnosed as having PNP between 1992 and 2010. MAIN OUTCOME MEASURES: Overall Kaplan-Meier survival rates were estimated, and features associated with survival were assessed using univariate (log-rank test) and multivariate (Cox regression) analyses...
October 2012: Archives of Dermatology
https://www.readbyqxmd.com/read/22715825/varicella-zoster-virus-vzv-and-alpha-1-antitrypsin-a-fatal-outcome-in-a-patient-affected-by-endemic-pemphigus-foliaceus
#12
Ana Maria Abreu Velez, Bruce R Smoller, Weiqing Gao, Hans E Grossniklaus, Zhe Jiao, Luis F Arias, Samuel C Dudley, Michael S Howard
BACKGROUND: Herpes virus infections are well known infectious complications of pemphigus and bullous pemphigoid. We describe pathologic findings utilizing autopsy tissue from several organs from a patient affected by a new variant of endemic pemphigus in El Bagre, Colombia, South America. CASE REPORT: We describe a patient by a new variant of endemic pemphigus foliaceus from El Bagre that was receiving high-dosage immunosuppressants when hospitalized and died suddenly following contact with a second patient affected by chicken pox...
July 2012: International Journal of Dermatology
https://www.readbyqxmd.com/read/22624125/endemic-pemphigus-over-a-century-part-ii
#13
Ana María Abréu-Vélez, Ana Maria Roselino, Michael S Howard, Iara J de Messias Reason
BACKGROUND: Endemic pemphigus foliaceus (EPF) is an autoimmune disease, classically occurring in a restricted geographic area. Foci of EPF have been described in several Central and South American countries, often affecting young people and Amerindians, with some female predilection. Although most American EPF cases have been documented in Brazil, cases have been reported in Peru, Paraguay, El Salvador and Venezuela. An additional variant of EPF has been described in El Bagre, Colombia, (El Bagre-EPF) affecting older men and a few post-menopausal females...
March 2010: North American Journal of Medical Sciences
https://www.readbyqxmd.com/read/22277944/pathogenic-anti-desmoglein-3-mabs-cloned-from-a-paraneoplastic-pemphigus-patient-by-phage-display
#14
Marwah A Saleh, Ken Ishii, Jun Yamagami, Yuji Shirakata, Koji Hashimoto, Masayuki Amagai
Paraneoplastic pemphigus (PNP) is an autoimmune blistering disease associated with lymphoproliferative neoplasms and characterized by antibodies against plakins and desmoglein 3 (Dsg3). Anti-Dsg3 antibodies have a primary role in blister formation in PNP. In this study, we used phage display to clone monoclonal anti-Dsg3 antibodies from a PNP patient to further characterize their pathogenicity. We isolated 20 unique Dsg3-reactive mAbs, which we classified into four groups according to the heavy-chain complementarity-determining region 3 (CDR3) region...
April 2012: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/21936565/map1b-and-clathrin-are-novel-interacting-partners-of-the-giant-cyto-linker-dystonin
#15
Kunal Bhanot, Kevin G Young, Rashmi Kothary
Dystonin is a large multidomain cytoskeletal-associated protein that plays an essential role in the nervous system. Loss of dystonin results in neuromuscular dysfunction and early death in a mouse mutant called dystonia musculorum. Conserved among related proteins, the plakin domain is a defining feature of all major dystonin isoforms, yet its interactions have not been explored in detail. The purpose of the present study was to identify novel interacting partners of the plakin domain of the neuronal isoform of dystonin (dystonin-a)...
November 4, 2011: Journal of Proteome Research
https://www.readbyqxmd.com/read/21796504/cardiac-autoantibodies-from-patients-affected-by-a-new-variant-of-endemic-pemphigus-foliaceus-in-colombia-south-america
#16
Ana Maria Abreu-Velez, Michael S Howard, Zhe Jiao, Weiqing Gao, Hong Yi, Hans E Grossniklaus, Mauricio Duque-Ramírez, Samuel C Dudley
Several patients affected by a new variant of endemic pemphigus foliaceus in El Bagre, Colombia (El Bagre-EPF) have experienced a sudden death syndrome, including persons below the age of 50. El Bagre-EPF patients share several autoantigens with paraneoplastic pemphigus patients, such as reactivity to plakins. Further, paraneoplastic pemphigus patients have autoantibodies to the heart. Therefore, we tested 15 El Bagre-EPF patients and 15 controls from the endemic area for autoreactivity to heart tissue using direct and indirect immunofluorescence, confocal microscopy, immunohistochemistry, immunoblotting, and immunoelectron microscopy utilizing heart extracts as antigens...
December 2011: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/20507390/immunoglobulin-a-antibodies-against-desmoglein-1-envoplakin-periplakin-and-bp230-in-a-patient-with-atypical-bullous-pemphigoid
#17
Fumi Yamaki, Nobuyasu Mayuzumi, Shigaku Ikeda, Takashi Hashimoto
Bullous pemphigoid is an autoimmune subepidermal blistering disease associated with autoantibodies against BP180 and BP230. We report herein a rare case of bullous pemphigoid with newly formed annular erythematous lesions when bullous skin lesions were in remission. Various immunological studies revealed immunoglobulin (Ig)A antibodies against desmoglein 1, envoplakin, periplakin and BP230 in addition to IgG antibodies against BP180 and BP230. These clinical and immunological changes in a patient are a rare event, suggesting an epitope-spreading phenomenon...
March 2010: Journal of Dermatology
https://www.readbyqxmd.com/read/20153198/crt-1-calreticulin-and-the-e3-ligase-eel-1-huwe1-control-hemidesmosome-maturation-in-c-elegans-development
#18
Hala Zahreddine, Huimin Zhang, Marie Diogon, Yasuko Nagamatsu, Michel Labouesse
Hemidesmosomes connect the extracellular matrix (ECM) to intermediate filaments through ECM receptors and plakins (plectin and BPAG1e). They affect tissue integrity, wound healing, and carcinoma invasion. Although biochemical and time-lapse studies indicate that alpha6beta4-integrin (ECM receptor) and plectin play a central role in modulating hemidesmosome disassembly, the mechanisms controlling hemidesmosome biogenesis in vivo remain poorly understood. The nematode C. elegans provides a powerful genetic model to address this issue...
February 23, 2010: Current Biology: CB
https://www.readbyqxmd.com/read/20071462/p21-activated-kinases-regulate-actin-remodeling-in-glomerular-podocytes
#19
Jianxin Zhu, Ortal Attias, Lamine Aoudjit, Ruihua Jiang, Hiroshi Kawachi, Tomoko Takano
The tyrosine phosphorylation of nephrin is reported to regulate podocyte morphology via the Nck adaptor proteins. The Pak family of kinases are regulators of the actin cytoskeleton and are recruited to the plasma membrane via Nck. Here, we investigated the role of Pak in podocyte morphology. Pak1/2 were expressed in cultured podocytes. In mouse podocytes, Pak2 was predominantly phosphorylated, concentrated at the tips of the cellular processes, and its expression and/or phosphorylation were further increased when differentiated...
April 2010: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/19737550/development-of-elisa-for-the-specific-determination-of-autoantibodies-against-envoplakin-and-periplakin-in-paraneoplastic-pemphigus
#20
Christian Probst, Wolfgang Schlumberger, Winfried Stöcker, Andreas Recke, Enno Schmidt, Takashi Hashimoto, Xue Jun Zhu, Detlef Zillikens, Lars Komorowski
INTRODUCTION: In paraneoplastic pemphigus (PNP), indirect immunofluorescence microscopy using rat bladder sections is widely used to search for circulating autoantibodies which are predominantly directed against envoplakin and periplakin. A sensitive and specific detection system for envoplakin/periplakin-specific autoantibodies is not yet available. METHODS: Overlapping fragments spanning envoplakin and periplakin, respectively, were analyzed for their ability to bind PNP-specific autoantibodies by immunoblotting and ELISA in sera from patients with PNP (n=31), pemphigus vulgaris (n=30), and bullous pemphigoid (n=50) as well as healthy volunteers (n=140)...
December 2009: Clinica Chimica Acta; International Journal of Clinical Chemistry
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