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https://www.readbyqxmd.com/read/28339937/uterine-artery-leptin-receptors-during-the-ovarian-cycle-and-pregnancy-regulate-angiogenesis-in-ovine-uterine-artery-endothelial-cells
#1
Vladimir E Vargas, Rosalina Villalon Landeros, Gladys E Lopez, Jing Zheng, Ronald R Magness
Leptin regulates body weight, reproductive functions, blood pressure, endothelial function, and fetoplacental angiogenesis. Compared to the luteal phase, the follicular phase and pregnancy are physiological states of elevated estrogen, angiogenesis, and uterine blood flow (UBF). Little is known concerning leptin and its receptors (OB-R) for regulating uterine artery (UA) angiogenesis. We : 1) Ex vivo expression of OB-R in UA endothelium (UAendo) and UA vascular smooth muscle (UAvsm) is elevated in pregnant vs...
March 1, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/28333983/alterations-in-amino-acid-status-in-cats-with-feline-dysautonomia
#2
Bruce C McGorum, Herb W Symonds, Clare Knottenbelt, Tom A Cave, Susan J MacDonald, Joanna Stratton, Irene Leon, Judith A Turner, R Scott Pirie
Feline dysautonomia (FD) is a multiple system neuropathy of unknown aetiology. An apparently identical disease occurs in horses (equine grass sickness, EGS), dogs, rabbits, hares, sheep, alpacas and llamas. Horses with acute EGS have a marked reduction in plasma concentrations of the sulphur amino acids (SAA) cyst(e)ine and methionine, which may reflect exposure to a neurotoxic xenobiotic. The aim of this study was to determine whether FD cats have alterations in amino acid profiles similar to those of EGS horses...
2017: PloS One
https://www.readbyqxmd.com/read/28333616/cross-sectional-surveillance-of-middle-east-respiratory-syndrome-coronavirus-mers-cov-in-dromedary-camels-and-other-mammals-in-egypt-august-2015-to-january-2016
#3
Mohamed Ali, Rabeh El-Shesheny, Ahmed Kandeil, Mahmoud Shehata, Basma Elsokary, Mokhtar Gomaa, Naglaa Hassan, Ahmed El Sayed, Ahmed El-Taweel, Heba Sobhy, Folorunso Oludayo Fasina, Gwenaelle Dauphin, Ihab El Masry, Abebe Wossene Wolde, Peter Daszak, Maureen Miller, Sophie VonDobschuetz, Emma Gardner, Subhash Morzaria, Juan Lubroth, Yilma Jobre Makonnen
A cross-sectional study was conducted in Egypt to determine the prevalence of Middle East respiratory syndrome coronavirus (MERS-CoV) in imported and resident camels and bats, as well as to assess possible transmission of the virus to domestic ruminants and equines. A total of 1,031 sera, 1,078 nasal swabs, 13 rectal swabs, and 38 milk samples were collected from 1,078 camels in different types of sites. In addition, 145 domestic animals and 109 bats were sampled. Overall, of 1,031 serologically-tested camels, 871 (84...
March 16, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28333110/stakeholder-perceptions-of-welfare-issues-and-indicators-for-extensively-managed-sheep-in-australia
#4
Amanda K Doughty, Grahame J Coleman, Geoff N Hinch, Rebecca E Doyle
An online survey was designed to form the basis of a framework for the welfare assessment of extensively managed sheep in Australia. The survey focused on welfare compromise and useful welfare indicators. A total of 952 people completed the survey in its entirety, representing four stakeholder groups: Public (53.6%), Producer (27.4%), Scientist (9.9%), and Service provider (9.1%). Animal welfare was considered to be important by all participating groups in this survey (average score of 3.78/4). Respondents felt the welfare of grazing sheep was generally adequate but improvement was desired (2...
March 23, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/28332740/ultrasound-during-mid-gestation-agreement-with-physical-foetal-and-placental-measurements-and-use-in-predicting-gestational-age-in-sheep
#5
A K Jones, R E Gately, K K McFadden, M L Hoffman, S M Pillai, S A Zinn, K E Govoni, S A Reed
To determine the effects of poor maternal nutrition and litter size on foetal growth during mid-gestation, pregnant ewes (n = 82) were fed 100%, 60% or 140% of NRC TDN beginning at day 30.2 ± 0.2 of gestation. Transabdominal ultrasound was performed weekly between day 46.0 ± 0.4 and 86.0 ± 0.7 to monitor foetal heart width (HW), umbilical diameter (UMB), rib width (RW) and placentome outer (OD) and inner diameter (ID). Data were analysed with repeated-measures using the mixed procedure for effects of maternal diet, litter size and gestation, and equations predictive of gestational age were generated using the regression procedure...
March 23, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28332653/review-of-the-foot-and-mouth-disease-situation-in-north-africa-and-the-risk-of-introducing-the-disease-into-europe
#6
R Bouguedour, A Ripani
Foot and mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals, including cattle, pigs, sheep, goats, and certain wildlife species. The disease can cause massive economic losses when introduced into countries that were free from the infection, generating negative effects due to reduced animal productivity and restrictions on international livestock trade. Following 15 years of FMD absence, Tunisia and Algeria experienced an incursion of the disease in 2014. The epidemiological situation and disease control measures in operation for FMD in the North African region are not homogeneous...
December 2016: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/28332652/the-impact-of-importation-of-live-ruminants-on-the-epizootiology-of-foot-and-mouth-disease-in-saudi-arabia
#7
I H A Abd El-Rahim, A H Asghar, A M Mohamed, S M Fat'hi
Approximately five million live ruminants are imported annually into Saudi Arabia. The majority of these animals are imported shortly before the pilgrimage season from Sudan and the Horn of Africa, where foot and mouth disease (FMD) is known to be enzootic. This study was designed to investigate the impact of the importation of these live ruminants on the epizootiology of FMD in Saudi Arabia. The authors carried out antibody testing on a total of 480 sheep and 233 cattle from the sacrificial livestock yards of the Saudi Project for Utilization of Hajj Meat, which performs ritual slaughter on behalf of pilgrims in the Holy City of Makkah...
December 2016: Revue Scientifique et Technique
https://www.readbyqxmd.com/read/28330747/m-protein-is-sufficient-for-assembly-and-release-of-peste-des-petits-ruminants-virus-like-particles
#8
Qiuxia Wang, Changbo Ou, Yongxi Dou, Lei Chen, Xuelian Meng, Xingyou Liu, Yan Yu, Jinqing Jiang, Jinyou Ma, Zhidong Zhang, Jianhe Hu, Xuepeng Cai
Peste des petits ruminants virus (PPRV), belonging to paramyxoviruses, has six structure proteins (such as matrix protein (M), nucleocapsid proteins (N), fusion protein (F) and hemagglutinin protein (H)) and could cause high morbidity and mortality in sheep and goats. Although a vaccine strain of PPRV has been rescued and co-expression of M and N could yield PPRV-like particles, the roles of structure proteins in virion assembly and release have not been investigated in detail. In this study, plasmids carrying PPRV cDNA sequences encoding the N, M, H, and F proteins were expressed in Vero cells...
March 18, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28330726/provision-of-physiological-data-and-reference-values-in-awake-and-anaesthetized-female-sheep-aged-6-12-months
#9
Tim Kampmeier, Philip Arnemann, Michael Heßler, Sebastian Rehberg, Andrea Morelli, Martin Westphal, Matthias Lange, Hugo Van Aken, Christian Ertmer
OBJECTIVE: To provide physiological data and reference values in awake and anaesthetized sheep aged 6-12 months. STUDY DESIGN: Descriptive study. ANIMALS: Data from 260 female sheep of the species Ovis orientalis aries aged 6-12 months were extracted from 10 experimental trials. METHODS: Data from pulmonary arterial thermodilution in awake (cohort 1; n = 109) and anaesthetized animals (cohort 2; n = 81), and transpulmonary thermodilution in anaesthetized animals (cohort 3; n = 70) were analysed...
January 11, 2017: Veterinary Anaesthesia and Analgesia
https://www.readbyqxmd.com/read/28329164/large-animal-model-of-functional-tricuspid-regurgitation-in-pacing-induced-end-stage-heart-failure
#10
Marcin Malinowski, Alistair G Proudfoot, David Langholz, Lenora Eberhart, Michael Brown, Hans Schubert, Jeremy Wodarek, Tomasz A Timek
OBJECTIVES: Functional tricuspid regurgitation (FTR) is common in patients with advanced heart failure and frequently complicates left ventricular assist device implantation yet remains poorly understood. We set out to establish large animal model of FTR that could serve as a research platform to investigate the pathogenesis of FTR associated with end-stage heart failure. METHODS: : Through right thoracotomy, ten adult sheep underwent implantation of pacemaker with epicardial LV lead, five sonomicrometry crystals on the right ventricle, and left and right ventricular telemetry pressure sensors during a beating heart off-pump procedure...
February 21, 2017: Interactive Cardiovascular and Thoracic Surgery
https://www.readbyqxmd.com/read/28328240/insights-on-cryopreserved-sheep-fibroblasts-by-cryomicroscopy-and-gene-expression-analysis
#11
Antonella Idda, Daniela Bebbere, Giuseppina Corona, Laura Masala, Elisa Casula, Alberto Cincotti, Sergio Ledda
Cryopreservation includes a set of techniques aimed at storing biological samples and preserving their biochemical and functional features without any significant alterations. This study set out to investigate the effects induced by cryopreservation on cultured sheepskin fibroblasts (CSSF) through cryomicroscopy and gene expression analysis after subsequent in vitro culture. CSSF cells were cryopreserved in a cryomicroscope (CM) or in a straw programmable freezer (SPF) using a similar thermal profile (cooling rate -5°C/min to -120°C, then -150°C/min to -196°C)...
March 22, 2017: Biopreservation and Biobanking
https://www.readbyqxmd.com/read/28328155/does-dynorphin-play-a-role-in-the-onset-of-puberty-in-female-sheep
#12
J A Lopez, M N Bedenbaugh, R B McCosh, P W Weems, L J Meadows, B Wisman, L M Coolen, R L Goodman, S M Hileman
Puberty onset involves increased gonadotrophin-release (GnRH) release as a result of decreased sensitivity to oestrogen (E2 )-negative feedback. Because GnRH neurones lack E2 receptor α, this pathway must contain interneurones. One likely candidate is KNDy neurones (kisspeptin, neurokinin B, dynorphin). The overarching hypothesis of the present study was that the prepubertal hiatus in luteinising hormone (LH) release involves reduced kisspeptin and/or heightened dynorphin input. We first tested the specific hypothesis that E2 would reduce kisspeptin-immunopositive cell numbers and increase dynorphin-immunopositive cell numbers...
December 2016: Journal of Neuroendocrinology
https://www.readbyqxmd.com/read/28327211/reduced-fetal-vitamin-d-status-by-maternal-undernutrition-during-discrete-gestational-windows-in-sheep
#13
J K Cleal, M R Hargreaves, K R Poore, J C Y Tang, W D Fraser, M A Hanson, L R Green
Placental transport of vitamin D and other nutrients (e.g. amino acids, fats and glucose) to the fetus is sensitive to maternal and fetal nutritional cues. We studied the effect of maternal calorific restriction on fetal vitamin D status and the placental expression of genes for nutrient transport [aromatic T-type amino acid transporter-1 (TAT-1); triglyceride hydrolase/lipoprotein uptake facilitator lipoprotein lipase (LPL)] and vitamin D homeostasis [CYP27B1; vitamin D receptor (VDR)], and their association with markers of fetal cardiovascular function and skeletal muscle growth...
March 22, 2017: Journal of Developmental Origins of Health and Disease
https://www.readbyqxmd.com/read/28326711/-comparative-experiments-of-stripping-sheep-maxillary-sinus-mucosal-with-new-type-stripper-and-umbrella-detacher
#14
Fan Jiadong, Li Yanfeng, Liu Le, Han Yishi, Hu Pin, Zhang Yue, Bao Wenya
OBJECTIVE: To compare the stripping length obtained through the model of 0.012 and 0.014 of the nickel-titanium dual-stage maxillary sinus mucosa stripper, and the umbrella detacher. METHODS: Twenty-four goats (1.5-2 years old) were chosen, randomly divided into A, B, C groups, each group of eight. Group A was 0.012 model group, group B was 0.014 model group, group C was umbrella detacher group. Animal model was established, and maxillary sinus mucosa was stripped to the left and right sides of the bottom of the sinus in three groups...
October 1, 2016: Hua Xi Kou Qiang Yi Xue za Zhi, Huaxi Kouqiang Yixue Zazhi, West China Journal of Stomatology
https://www.readbyqxmd.com/read/28326632/reproductive-performance-of-postpartum-ewes-treated-with-insulin-or-progesterone-hormones-in-association-with-ram-effect
#15
J C Ferreira-Silva, Srl Basto, F Tenório Filho, M T Moura, M L Silva Filho, Mal Oliveira
The reproductive performance of postpartum Santa Inês (SI) and Morada Nova (MN) ewes treated with insulin or progesterone hormones in association with ram effect was evaluated. Ewes from SI (n = 69) and MN (n = 69) breeds were randomly allocated to three groups of each breed (T1-ram effect only; T2-ram effect + insulin; T3-ram effect + progesterone). Progesterone concentrations (ηg/ml; mean ± SD) before and after introduction of rams (n = 6) were 0.51 ± 0.22 and 3.78 ± 0.68 (T1), 0.65 ± 0...
March 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28325734/does-maternal-fetal-transfer-of-creatine-occur-in-pregnant-sheep
#16
Syed Baharom, Robert De Matteo, Stacey Ellery, Paul Della Gatta, Clinton R Bruce, Greg M Kowalski, Nadia Hale, Hayley Dickinson, Richard Harding, David Walker, Rodney J Snow
Our aim was to determine the disposition of creatine in ovine pregnancy, and whether creatine is transferred across the placenta from mother to fetus. Pregnant ewes received either: (i) a continuous intravenous infusion of creatine monohydrate or saline from 122 to 131 days gestation, with maternal and fetal arterial blood and amniotic fluid samples collected daily for creatine analysis and fetal tissues collected at necropsy at 133 days for analysis of creatine content, or; (ii) a single systemic bolus injection of (13)C labelled creatine monohydrate at 130 days of gestation, with maternal and fetal arterial blood, uterine vein blood and amniotic fluid samples collected prior to, and for 4 hours post injection and analysed for creatine, creatine isotopic enrichment, and guanidinoacetic acid (GAA, precursor of creatine) concentrations...
March 21, 2017: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/28324006/effects-of-season-and-estradiol-on-kndy-neuron-peptides-colocalization-with-d2-dopamine-receptors-and-dopaminergic-inputs-in-the-ewe
#17
Peyton Weems, Jeremy Smith, Iain J Clarke, Lique M Coolen, Robert L Goodman, Michael N Lehman
Seasonal reproduction in sheep is primarily due to a dramatic increase in the ability of estradiol (E2) to inhibit the pulsatile secretion of gonadotropin-releasing hormone (GnRH) during the non-breeding season (anestrus). Recent findings suggest that KNDy (kisspeptin/neurokinin B/dynorphin) neurons of the arcuate nucleus (ARC) play a key role in conveying this negative feedback influence, with dopaminergic projections from the retrochiasmatic area acting upon KNDy cells to decrease kisspeptin release and thus inhibit GnRH pulses...
January 16, 2017: Endocrinology
https://www.readbyqxmd.com/read/28323564/differential-susceptibility-of-bighorn-sheep-ovis-canadensis-and-domestic-sheep-ovis-aries-neutrophils-to-mannheimia-haemolytica-leukotoxin-is-not-due-to-differential-expression-of-cell-surface-cd18
#18
Rohana P Dassanayake, Sudarvili Shanthalingam, Weiguo Liu, Eduardo Casas, Subramaniam Srikumaran
Bighorn sheep ( Ovis canadensis ) are more susceptible to pneumonia caused by Mannheimia haemolytica than are domestic sheep ( Ovis aries ). Leukotoxin produced by M. haemolytica is the principal virulence factor involved in pneumonia pathogenesis. Although leukotoxin is cytolytic to all subsets of ruminant leukocytes, neutrophils are the most susceptible subset. Bighorn sheep neutrophils are 4- to 8-fold more susceptible to leukotoxin-induced cytolysis than are domestic sheep neutrophils. We hypothesized that the higher susceptibility of bighorn sheep neutrophils, in comparison to domestic sheep neutrophils, is due to higher expression of CD18, the receptor for leukotoxin on leukocytes...
March 21, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28323562/corynebacterium-pseudotuberculosis-infection-in-patagonian-huemul-hippocamelus-bisulcus
#19
Nelly Morales, Dennis Aldridge, Andrea Bahamonde, Julio Cerda, Claudio Araya, Rodrigo Muñoz, María Esther Saldías, Claudio Lecocq, Marcela Fresno, Pedro Abalos, Patricio Retamal
Corynebacterium pseudotuberculosis is an intracellular bacteria and the etiologic agent of caseous lymphadenitis in domestic and wildlife species. We report C. pseudotuberculosis infection in Patagonian huemul ( Hippocamelus bisulcus ) from the Cerro Castillo National Reserve, Region of Aysen, Chile. Subcutaneous abscesses in the abdominal and pectoral regions from two animals were sampled and bacteriologic isolation was performed. In both cases, we isolated a C. pseudotuberculosis strain belonging to the ovine genotype...
March 21, 2017: Journal of Wildlife Diseases
https://www.readbyqxmd.com/read/28323135/effect-of-bioaugmentation-by-cellulolytic-bacteria-enriched-from-sheep-rumen-on-methane-production-from-wheat-straw
#20
E Gozde Ozbayram, Sabine Kleinsteuber, Marcell Nikolausz, Bahar Ince, Orhan Ince
The aim of this study was to determine the potential of bioaugmentation with cellulolytic rumen microbiota to enhance the anaerobic digestion of lignocellulosic feedstock. An anaerobic cellulolytic culture was enriched from sheep rumen fluid using wheat straw as substrate under mesophilic conditions. To investigate the effects of bioaugmentation on methane production from straw, the enrichment culture was added to batch reactors in proportions of 2% (Set-1) and 4% (Set-2) of the microbial cell number of the standard inoculum slurry...
March 17, 2017: Anaerobe
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