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https://www.readbyqxmd.com/read/28446763/hanging-drop-a-best-three-dimensional-3d-culture-method-for-primary-buffalo-and-sheep-hepatocytes
#1
Meena Shri, Himanshu Agrawal, Payal Rani, Dheer Singh, Suneel Kumar Onteru
Livestock, having close resemblance to humans, could be a better source of primary hepatocytes than rodents. Herein, we successfully developed three-dimensional (3D) culturing system for primary sheep and buffalo hepatocytes. The 3D-structures of sheep hepatocytes were formed on the fifth-day and maintained until the tenth-day on polyHEMA-coated plates and in hanging drops with William's E media (HDW). Between the cultured and fresh cells, we observed a similar expression of GAPDH, HNF4α, ALB, CYP1A1, CK8 and CK18...
April 26, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28444752/prevalence-and-pathogen-load-of-campylobacter-spp-salmonella-enterica-and-escherichia-coli-o157-o145-serogroup-in-sheep-faeces-collected-at-sale-yards-and-in-abattoir-effluent-in-western-australia
#2
R Yang, S Abraham, G E Gardner, U Ryan, C Jacobson
OBJECTIVE: Develop a multiplex quantitative PCR assay to investigate the prevalence and shedding of Escherichia coli O157/O145, Salmonella spp. and Campylobacter spp. in sheep at sale yards and abattoirs. METHODS: A qPCR for E. coli O157/O145 was developed, validated and multiplexed with an existing qPCR for Campylobacter and Salmonella enterica. The absolute numbers of E. coli O157/O145, Campylobacter and Salmonella in control samples was determined using droplet digital PCR...
May 2017: Australian Veterinary Journal
https://www.readbyqxmd.com/read/28444750/in-this-issue-may-2017-mutation-in-droughtmaster-cattle%C3%A2-%C3%A2-%C3%A2-measurement-of-zoonotic-pathogens-in-sheep%C3%A2-%C3%A2-%C3%A2-lymphoma-in-australian-dog-breeds%C3%A2-%C3%A2-%C3%A2-rapid-treatment-needed-in-cats-with-hypocobalinaemia%C3%A2-%C3%A2-%C3%A2-lockable-hinge-struts-for-salter-harris-fractures%C3%A2
#3
EDITORIAL
https://www.readbyqxmd.com/read/28443528/understanding-medusahead-low-intake-and-palatability-through-in-vitro-digestibility-and-fermentation-kinetics
#4
J J Montes-Sánchez, J J Villalba
The low intake of medusahead grass (Taeniatherum caput-medusae ssp. asperum) by sheep is attributed to the high silica content of the plant and its negative impact on digestibility, making this weed a successful competitor in grazed plant communities. The goals of this study were to determine the influence of (1) plant maturity stage (from late vegetative stage to beginning of senescence and thatch), (2) particle size (1, 5, 10 and 20 mm), and (3) high-quality feeds (alfalfa hay and a high-energy concentrate) on fermentation kinetics and apparent digestibility of medusahead relative to palatable feeds (alfalfa and tall fescue hays, and high-energy concentrates)...
April 26, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28442051/cerium-oxide-nanoparticles-ceo2-nps-improve-the-developmental-competence-of-in-vitro-matured-prepubertal-ovine-oocytes
#5
F Ariu, L Bogliolo, A Pinna, L Malfatti, P Innocenzi, L Falchi, D Bebbere, S Ledda
The present study investigated whether supplementation with different doses of cerium dioxide nanoparticles (CeO2 NPs) during in vitro maturation (IVM) of prepubertal ovine oocytes influenced their embryonic development in vitro. Cumulus-oocyte complexes derived from the ovaries of slaughtered prepubertal sheep underwent IVM with CeO2NPs (0, 44, 88 or 220µg mL(-1)). Matured oocytes were fertilised in vitro and zygotes were cultured for 7 days. The results demonstrated that CeO2NPs were internalised in the cumulus cells and not in the oocyte...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28442041/cytokine-gene-expression-at-the-maternal-fetal-interface-after-somatic-cell-nuclear-transfer-pregnancies-in-small-ruminants
#6
Heloisa M Rutigliano, Amanda Wilhelm, Justin Hall, Bi Shi, Qinggang Meng, Rusty Stott, Thomas D Bunch, Kenneth L White, Christopher J Davies, Irina A Polejaeva
The present retrospective study investigated pregnancy rates, the incidence of pregnancy loss and large offspring syndrome (LOS) and immune-related gene expression of sheep and goat somatic cell nuclear transfer (SCNT) pregnancies. We hypothesised that significantly higher pregnancy losses observed in sheep compared with goat SCNT pregnancies are due to the increased amounts of T-helper 1 cytokines and proinflammatory mediators at the maternal-fetal interface. Sheep and goat SCNT pregnancies were generated using the same procedure...
April 2017: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/28441680/magnetic-resonance-imaging-safety-of-magnetically-controlled-growing-rods-in-an-in-vivo-animal-model
#7
Mehmet Eroglu, Gokhan Demirkiran, Ismail Aykut Kocyigit, Hasan Bilgili, Mehmet Burak Kaynar, Ali Bumin, Sadan Ozcan, Muharrem Yazici
STUDY DESIGN: Experimental animal study. OBJECTIVE: To investigate the interaction between magnetically controlled growing rods (MCGRs) and magnetic resonance imaging (MRI). SUMMARY OF BACKGROUND DATA: Growing rod treatment through serial operations results in adverse effects on the patient and high treatment costs. MCGRs can be lengthened noninvasively in an outpatient setting and with lower treatment costs. When MRI investigation is required, the interaction between MCGRs and MRI is an issue of concern in patients with MCGRs...
May 1, 2017: Spine
https://www.readbyqxmd.com/read/28441662/buffalo-leukemia-inhibitory-factor-induces-differentiation-and-dome-like-secondary-structures-in-cos-1-cells
#8
Gurjeet Kaur, Syed Azmal Ali, Shikha Pachauri, Dhruba Malakar, Jai K Kaushik, Ashok K Mohanty, Sudarshan Kumar
This study aimed to understand the molecular characteristics of buffalo leukemia inhibitory factor (BuLIF) and the generation of a stably transfected COS-1_BuLIF cell line for its functional characterization. Cumulus cells, isolated from oocytes, were separated, and total cDNA was prepared. The BuLIF gene was ligated into the cloning vector pJET1.2/blunt and expression vector pAcGFP-N1 which was transfected into COS-1 cells and confirmed by qRT-PCR and Western blot. BuLIF was immunoprecipitated and evaluated through a MTT assay...
April 26, 2017: Cytogenetic and Genome Research
https://www.readbyqxmd.com/read/28441576/fate-and-redistribution-of-perfluoroalkyl-acids-through-afff-impacted-groundwater
#9
Jennifer Bräunig, Christine Baduel, Amy Heffernan, Anna Rotander, Eric Donaldson, Jochen F Mueller
Leaching of perfluoroalkyl acids (PFAAs) from a local point source, a fire-fighting training area, has led to extensive contamination of a groundwater aquifer which has spread underneath part of a nearby town, Oakey, situated in the State of Queensland, Australia. Groundwater is extracted by residents from privately owned wells for daily activities such as watering livestock and garden beds. The concentration of 10 PFAAs in environmental and biological samples (water, soil, grass, chicken egg yolk, serum of horses, cattle and sheep), as well as human serum was investigated to determine the extent of contamination in the town and discuss fate and redistribution of PFAAs...
April 22, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28441332/pregnant-sheep-in-a-farm-environment-did-not-develop-anaemia
#10
Gabrielle C Musk, Amanda James, Matthew W Kemp, Sara Ritchie, Andrew Ritchie, Michael Laurence
The aim of this study was to document the haematological profile of pregnant ewes throughout gestation. Sheep were divided into three groups (n = 8 per group): non-pregnant, singleton, or twin pregnancy. Blood samples were collected every 14 days from day 55 of gestation for haemoglobin concentration; packed cell volume; total protein; and albumin concentration. On days 55 and 125 of gestation blood was collected for trace element estimation: soluble copper and zinc; glutathione peroxidase (GSHPx); and methylmalonic acid (MMA)...
April 25, 2017: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/28441044/pharmacokinetics-and-pharmacodynamics-of-midazolam-following-intravenous-and-intramuscular-administration-to-sheep
#11
Bradley T Simon, Elizabeth M Scallan, Odette O, Lisa Sams Ebner, Michelle N Cerullo, Christelle Follette, Sherry K Cox, Thomas J Doherty, Ignacio Lizarraga
OBJECTIVE To determine the pharmacokinetic and pharmacodynamic effects of midazolam following IV and IM administration in sheep. ANIMALS 8 healthy adult rams. PROCEDURES Sheep were administered midazolam (0.5 mg/kg) by the IV route and then by the IM route 7 days later in a crossover study. Physiologic and behavioral variables were assessed and blood samples collected for determination of plasma midazolam and 1-hydroxymidazolam (primary midazolam metabolite) concentrations immediately before (baseline) and at predetermined times for 1,440 minutes after midazolam administration...
May 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28439905/der-f-35-an-md-2-like-house-dust-mite-allergen-that-cross-reacts-with-der-f-2-and-pso-o-2
#12
Takashi Fujimura, Tsunehiro Aki, Toshihide Isobe, Akito Matsuoka, Takaharu Hayashi, Kazuhisa Ono, Seiji Kawamoto
BACKGROUND: Dermatophagoides farinae is a source of airborne house dust mite (HDM) allergens. We elucidated IgE-reactive allergens from D. farinae by two-dimensional immunoblotting-based allergenome analysis, and identified one new allergen, named Der f 35, that possesses IgE-binding capacity comparable to that of Der f 2. The aim of this study is to clarify the allergenic capacity of new HDM allergen Der f 35. METHODS: We cloned der f 35 from D. farinae mRNA and produced recombinant Der f 35 in Escherichia coli...
April 25, 2017: Allergy
https://www.readbyqxmd.com/read/28439265/bacterial-and-fungal-community-structures-in-loess-plateau-grasslands-with-different-grazing-intensities
#13
Huhe, Xianjiang Chen, Fujiang Hou, Yanpei Wu, Yunxiang Cheng
The Loess Plateau of China is one of the most fragile ecosystems worldwide; thus, human production activities need to be conducted very cautiously. In this study, MiSeq high-throughput sequencing was applied to assess the relationship between bacterial and fungal community structures and changes in vegetation and soil physical and chemical properties induced by grazing, in four grasslands with different levels of grazing intensity (0, 2.67, 5.33, and 8.67 sheep/ha) in the semiarid region of the Loess Plateau...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28438225/meta-analysis-of-the-parasitic-phase-traits-of-haemonchus-contortus-infection-in-sheep
#14
Mathilde Saccareau, Guillaume Sallé, Christèle Robert-Granié, Tom Duchemin, Philippe Jacquiet, Alexandra Blanchard, Jacques Cabaret, Carole R Moreno
BACKGROUND: The parasitic nematode Haemonchus contortus shows highly variable life history traits. This highlights the need to have an average estimate and a quantification of the variation around it to calibrate epidemiological models. METHODS: This paper aimed to quantify the main life history traits of H. contortus and to identify explanatory factors affecting these traits using a powerful method based on a systematic review and meta-analysis of current literature...
April 24, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28438095/arcobacter-an-emerging-food-borne-zoonotic-pathogen-its-public-health-concerns-and-advances-in-diagnosis-and-control-a-comprehensive-review
#15
Thadiyam Puram Ramees, Kuldeep Dhama, Kumaragurubaran Karthik, Ramswaroop Singh Rathore, Ashok Kumar, Mani Saminathan, Ruchi Tiwari, Yashpal Singh Malik, Raj Kumar Singh
Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries...
April 25, 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28436433/pathways-and-genes-associated-with-immune-dysfunction-in-sheep-paratuberculosis
#16
Anton Gossner, Craig Watkins, Francesca Chianini, John Hopkins
Multibacillary and paucibacillary paratuberculosis are both caused by Mycobacterium avium subspecies paratuberculosis. Multibacillary lesions are composed largely of infected epithelioid macrophages and paucibacillary lesions contain T cells but few bacteria. Multibacillary disease is similar to human lepromatous leprosy, with variable/high levels of antibody and a dysfunctional immune response. Animals with paucibacillary disease have high cell-mediated immunity and variable levels of antibody. This study aims to characterize the immunological dysfunction using TruSeq analysis of the ileocaecal lymph node that drains disease lesions...
April 24, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28436273/proteome-analysis-of-sheep-b-lymphocytes-in-the-course-of-bovine-leukemia-virus-induced-leukemia
#17
Michal Reichert
Presented are the results of a study of the expression pattern of different proteins in the course of bovine leukemia virus-induced leukemia in experimental sheep and I discuss how the obtained data may be useful in gaining a better understanding of the pathogenesis of the disease, diagnosis, and for the selection of possible therapeutic targets. In cattle, the disease is characterized by life-long persistent lymphocytosis leading to leukemia/lymphoma in about 5% of infected animals. In sheep, as opposed to cattle, the course of the disease is always fatal and clinical symptoms usually occur within a three-year period after infection...
January 1, 2017: Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28436039/nutrient-retention-values-and-cooking-yield-factors-for-three-south-african-lamb-and-mutton-cuts
#18
Salomina M van Heerden, Phillip E Strydom
BACKGROUND: Nutrient content of raw and cooked foods is important for formulation healthy diets. The retention of nutrients during cooking can be influenced by various factors including animal age, carcass characteristics and cooking method and these factors are often unique to specific countries. We studied the effects of animal age (lamb and mutton), carcass cut (shoulder, loin and leg) combined with cooking method (moist-heat and dry-heat) on yield and nutrient retention of selected nutrients of South African sheep carcasses...
April 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28435817/molecular-and-morphometrical-characterization-of-fasciola-species-isolated-from-domestic-ruminants-in-ardabil-province-northwestern-iran
#19
Mojgan Aryaeipour, Arezoo Bozorgomid, Bahram Kazemi, Masoud Behnia, Hakim Azizi, Mohammad Bagher Rokni
BACKGROUND: We aimed to describe morphological and morphometrical characteristics of Fasciola spp. in livestock from Ardabil Province, Northwest Iran. METHODS: Forty adult flukes were collected from different definitive hosts (cattle and sheep). Previously specimens were identified as F. hepatica or F. gigantica based on PCR-RFLP of the ITS-1 region with RsaI enzyme. We identified Fasciola spp. based on morphological and metric assessment of external features of fresh adults, morphological and metric assessment of internal anatomy of stained mounted worms...
March 2017: Iranian Journal of Public Health
https://www.readbyqxmd.com/read/28435188/effect-of-kisspeptin-on-in-vitro-maturation-of-sheep-oocytes
#20
Priyanka Byri, Arunakumari Gangineni, K Ramachandra Reddy, K B P Raghavender
AIM: The aim of this study was to investigate the effect of kisspeptin (KP) on in vitro maturation (IVM) of sheep oocytes aspirated from the ovaries collected from slaughterhouse. MATERIALS AND METHODS: Two different experiments were conducted to investigate the effect of KP (5, 10 and 15 µg/ml) alone (experiment 1) or in combination with follicle-stimulating hormone (FSH), luteinizing hormone (LH), and Estradiol (E2) (experiment 2) on IVM of sheep oocytes. Tissue culture medium 199 supplemented with Gentamicin was used as control medium...
March 2017: Veterinary World
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