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https://www.readbyqxmd.com/read/28936980/mir-101a-targeting-ezh2-promotes-the-differentiation-of-goat-skeletal-muscle-satellite-cells
#1
Jun-Tao Li, Wei Zhao, Dan-Dan Li, Jing Feng, Gui Ba, Tian-Zeng Song, Hong-Ping Zhang
miR-101a promotes the differentiation of goat skeletal muscle satellite cells (SMSCs), as we previously reported, but the underpinning mechanism remains to be illuminated. In this study, we predicted the target gene of miR-101a by employing online softwares PicTar, TargetScan and miRanda, and found that enhancer of zeste homologue 2 (EZH2) was targeted by miR-101a. Further we identified that EZH2 contained miR-101a binding sites at its 3'UTR by using the dual-luciferase reporter assay system. In addition, we showed that during SMSC differentiation, the downregulated levels of EZH2 mRNA and protein were accompanied by increasing miR-101a expression via qRT-PCR and Western blot...
September 20, 2017: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/28935604/molecular-features-and-physiological-roles-of-k-cl-cotransporter-4-kcc4
#2
REVIEW
A A Marcoux, A P Garneau, R Frenette-Cotton, S Slimani, F Mac-Way, P Isenring
A K(+)-Cl(-) cotransport system was documented for the first time during the mid-seventies in sheep and goat red blood cells. It was then described as a Na(+)-independent and ouabain-insensitive ion carrier that could be stimulated by cell swelling and N-ethylmaleimide (NEM), a thiol-reacting agent. Twenty years later, this system was found to be dispensed by four different isoforms in animal cells. The first one was identified in the expressed sequence tag (EST) database by Gillen et al. based on the assumption that it would be homologous to the Na(+)-dependent K(+)-Cl(-) cotransport system for which the molecular identity had already been uncovered...
September 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28934997/teatcups-with-automatic-valves-in-machine-milking-of-goats
#3
Alberto Manzur, José Ramón Díaz, Nemesio Fernández, Sebastià Balasch, Cristòfol Peris
This Research Paper addresses the hypothesis that using teatcups with automatic valves, without cutting off the vacuum prior to cluster removal, could increases the risk of mastitis and affect other milking variables on goats. A first trial used 46 intramammary infection (IMI)-free goats that had been milked with normal teatcups (without automatic valves) during a pre-experimental period of 8 ± 2 d postpartum. These animals were divided into two groups (n = 23), randomly assigning each group to teatcups with automatic valves (teatcups A) or without automatic valves (teatcups B) for a 20-week experimental period...
September 22, 2017: Journal of Dairy Research
https://www.readbyqxmd.com/read/28934943/identification-of-new-corynebacterium-pseudotuberculosis-antigens-by-immunoscreening-of-gene-expression-library
#4
Cleber Eduardo Galvão, Stenio Perdigão Fragoso, Carina Elisei de Oliveira, Odinéia Forner, Renata Ribeiro Bastos Pereira, Cleber Oliveira Soares, Grácia Maria Soares Rosinha
BACKGROUND: Caseous lymphadenitis (CLA) is a disease that affects sheep, goats and occasionally humans. The etiologic agent is the Corynebacterium pseudotuberculosis bacillus. The objective of this study was to build a gene expression library from C. pseudotuberculosis and use immunoscreening to identify genes that encode potential antigenic proteins for the development of DNA and subunit vaccines against CLA. RESULTS: A wild strain of C. pseudotuberculosis was used for extraction and partial digestion of genomic DNA...
September 21, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28934224/identification-and-analysis-of-differentially-expressed-long-non-coding-rnas-between-multiparous-and-uniparous-goat-capra-hircus-ovaries
#5
Yinghui Ling, Lina Xu, Long Zhu, Menghua Sui, Qi Zheng, Wenyong Li, Yong Liu, Fugui Fang, Xiaorong Zhang
Long non-coding RNAs (lncRNAs) play important roles in almost all biological processes. However, there is little information on the effects of lncRNAs on ovulation and lambing rates. In the present study, we used high-throughput RNA sequencing to identify differentially expressed lncRNAs between the ovaries of multiparous (Mul) and uniparous (Uni) Anhui White goats. Among the 107,255,422 clean reads, 183,754 lncRNAs were significantly differentially expressed between the Uni and Mul. Among them, 455 lncRNAs were co-expressed between the two samples, whereas, 157,523 lncRNAs were uniquely expressed in the Uni, and 25,776 uniquely lncRNAs were expressed in the Mul...
2017: PloS One
https://www.readbyqxmd.com/read/28933185/identification-and-distribution-of-the-interstitial-cells-of-cajal-in-the-abomasum-of-goats
#6
Lingling Wang, Yu Liang, Qiusheng Chen, Nisar Ahmed, Feng Wang, Bing Hu, Ping Yang
The interstitial cells of Cajal (ICCs) are regarded as pacemakers and are involved in neurotransmission in the gastrointestinal tract (GIT) of animals. However, limited information is available about the existence of ICCs within the GIT of ruminants. In this study, we investigated the ultrastructural characteristics and distribution of ICCs in goat abomasum using transmission electron microscopy and c-kit immunohistochemistry. Two different kinds of c-kit immunoreactive cells were observed in the abomasum. The first was identified as ICCs, which appeared to be multipolar or bipolar in shape, with some processes...
January 1, 2017: Cell Transplantation
https://www.readbyqxmd.com/read/28932217/molecular-epidemiology-of-cryptosporidiosis-in-china
#7
REVIEW
Yaoyu Feng, Lihua Xiao
Molecular epidemiology of cryptosporidiosis is an active research area in China. The use of genotyping and subtyping tools in prevalence studies has led to the identification of unique characteristics of Cryptosporidium infections in humans and animals. Human cryptosporidiosis in China is exemplified by the high diversity of Cryptosporidium spp. at species and subtype levels, with dominant C. hominis and C. parvum subtypes being rarely detected in other countries. Similarly, preweaned dairy calves, lambs, and goat kids are mostly infected with non-pathogenic Cryptosporidium species (C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28931951/screening-of-mirna-profiles-and-construction-of-regulation-networks-in-early-and-late-lactation-of-dairy-goat-mammary-glands
#8
Zhibin Ji, Zhaohua Liu, Tianle Chao, Lei Hou, Rui Fan, Rongyan He, Guizhi Wang, Jianmin Wang
In recent years, studies related to the expression profiles of miRNAs in the dairy goat mammary gland were performed, but regulatory mechanisms in the physiological environment and the dynamic homeostasis of mammary gland development and lactation are not clear. In the present study, sequencing data analysis of early and late lactation uncovered a total of 1,487 unique miRNAs, including 45 novel miRNA candidates and 1,442 known and conserved miRNAs, of which 758 miRNAs were co-expressed and 378 differentially expressed with P < 0...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28931067/in-vivo-imaging-system-for-explants-analysis-a-new-approach-for-assessment-of-cell-transplantation-effects-in-large-animal-models
#9
Weronika Zarychta-Wiśniewska, Anna Burdzinska, Radosław Zagozdzon, Bartosz Dybowski, Marta Butrym, Zdzisław Gajewski, Leszek Paczek
INTRODUCTION: Despite spectacular progress in cellular transplantology, there are still many concerns about the fate of transplanted cells. More preclinical studies are needed, especially on large animal models, to bridge the translational gap between basic research and the clinic. Herein, we propose a novel approach in analysis of cell transplantation effects in large animals explants using in vivo imaging system (IVIS®) or similar equipment. MATERIAL AND METHODS: In the in vitro experiment cells labeled with fluorescent membrane dyes: DID (far red) or PKH26 (orange) were visualized with IVIS®...
2017: PloS One
https://www.readbyqxmd.com/read/28930654/a-tale-of-two-genes-microglial-apoe-and-trem2
#10
Anna A Pimenova, Edoardo Marcora, Alison M Goate
Microglial cell function is implicated in the etiology of Alzheimer's disease by human genetics. In this issue of Immunity, Krasemann et al. (2017) describe a gene expression signature associated with an APOE- and TREM2-dependent response of microglia to brain tissue damage that accumulates in aging and disease, defining an axis that might be amenable to therapeutic targeting.
September 19, 2017: Immunity
https://www.readbyqxmd.com/read/28928415/cultivation-of-anaplasma-ovis-in-the-hl-60-human-promyelocytic-leukemia-cell-line
#11
Ran Wei, Hong-Bo Liu, Frans Jongejan, Bao-Gui Jiang, Qiao-Cheng Chang, Xue Fu, Jia-Fu Jiang, Na Jia, Wu-Chun Cao
The tick-borne bacterium Anaplasma ovis is a widely distributed pathogen affecting sheep, goats and wild ruminants. Here, the HL-60 human promyelocytic leukemia cell line was used to isolate A. ovis from PCR-positive sheep and goats in Heilongjiang Province, China. Two weeks after inoculation, morulae were observed in cytoplasmic vacuoles in four different HL-60 cultures. Confocal microscopy using a Cy3-labeled A. ovis-specific probe confirmed that the HL-60 cells were infected with A. ovis. Cells from the 6th HL-60 subculture displayed positive fluorescence when incubated with A...
September 20, 2017: Emerging Microbes & Infections
https://www.readbyqxmd.com/read/28927492/quantitative-detection-of-pork-contamination-in-cooked-meat-products-by-elisa
#12
Cortlandt P Thienes, Jongkit Masiri, Lora A Benoit, Brianda Barrios-Lopez, Santosh A Samuel, David P Cox, Anatoly P Dobritsa, Cesar Nadala, Mansour Samadpour
Recent news of many cases of adulteration of meats with pork has bolstered the need for a way to detect and quantify the unwanted contamination of pork in other meats. To address this need, Microbiologique, Inc. has produced a sandwich ELISA assay that can rapidly quantify the presence of pork in cooked horse, beef, chicken, goat, and lamb meats. We carried out a validation study and showed that this assay has an analytical sensitivity of 0.00014 and 0.00040% (w/v) for cooked and autoclaved pork, respectively, and an analytical range of quantitation of 0...
September 19, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28927478/molecular-hydrogen-generated-by-elemental-magnesium-supplementation-alters-rumen-fermentation-and-microbiota-in-goats
#13
Min Wang, Rong Wang, XiuMin Zhang, Emilio M Ungerfeld, Donglei Long, HongXiang Mao, JinZhen Jiao, Karen A Beauchemin, Zhiliang Tan
We tested the hypotheses that supplementation of a diet with elemental Mg increases ruminal dissolved H2 (dH2) in rumen fluid, which in turn alters rumen fermentation and microbial community in goats. In a randomised block design, twenty growing goats were allocated to two treatments fed the same basal diet with 1·45 % Mg(OH)2 or 0·6 % elemental Mg. After 28 d of adaptation, we collected total faeces to measure total tract digestibility, rumen contents to analyse fermentation end products and microbial groups, and measured methane (CH4) emission using respiration chambers...
September 20, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28927467/associations-among-dietary-non-fiber-carbohydrate-ruminal-microbiota-and-epithelium-g-protein-coupled-receptor-and-histone-deacetylase-regulations-in-goats
#14
Hong Shen, Zhongyan Lu, Zhihui Xu, Zhan Chen, Zanming Shen
BACKGROUND: Diet-derived short-chain fatty acids (SCFAs) in the rumen have broad effects on the health and growth of ruminants. The microbe-G-protein-coupled receptor (GPR) and microbe-histone deacetylase (HDAC) axes might be the major pathway mediating these effects. Here, an integrated approach of transcriptome sequencing and 16S rRNA gene sequencing was applied to investigate the synergetic responses of rumen epithelium and rumen microbiota to the increased intake of dietary non-fiber carbohydrate (NFC) from 15 to 30% in the goat model...
September 19, 2017: Microbiome
https://www.readbyqxmd.com/read/28927447/protecting-effect-of-prp-codons-m142-and-k222-in-goats-orally-challenged-with-bovine-spongiform-encephalopathy-prions
#15
C Fast, W Goldmann, P Berthon, K Tauscher, O Andréoletti, I Lantier, C Rossignol, A Bossers, J G Jacobs, N Hunter, M H Groschup, F Lantier, J P M Langeveld
Breeding towards genetic resistance to prion disease is effective in eliminating scrapie. In sheep, classical forms of scrapie have been eradicated almost completely in several countries by breeding programs using a prion protein (PrP) gene (PRNP) amino acid polymorphism. For goats, field and experimental studies have provided evidence for several amino acid polymorphisms that are associated with resistance to scrapie, but only limited data are available concerning the susceptibility of caprine PRNP genotypes to BSE...
September 19, 2017: Veterinary Research
https://www.readbyqxmd.com/read/28927427/first-molecular-detection-and-characterization-of-zoonotic-bartonella-species-in-fleas-infesting-domestic-animals-in-tunisia
#16
Saba Zouari, Fatma Khrouf, Youmna M'ghirbi, Ali Bouattour
BACKGROUND: Bartonellosis is an emerging vector-borne disease caused by different intracellular bacteria of the genus Bartonella (Rhizobiales: Bartonellaceae) that is transmitted primarily by blood-sucking arthropods such as sandflies, ticks and fleas. In Tunisia, there are no data available identifying the vectors of Bartonella spp. In our research, we used molecular methods to detect and characterize Bartonella species circulating in fleas collected from domestic animals in several of the country's bioclimatic areas...
September 19, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28924723/sixteen-chinese-pediatric-brucellosis-patients-onset-of-fever-in-non-epidemic-areas-and-8-developed-with-osteoarticular-involvement
#17
Xinning Wang, Yuchun Yan, Fengqi Wu, Gaixiu Su, Shengnan Li, Xinyu Yuan, Jianming Lai, Zhixuan Zhou
The purpose of this study is to summarize the manifestations, diagnosis, differential diagnosis, and treatment of childhood brucellosis in non-epidemic areas of China. A retrospective review of 16 admitted children patients with brucella's disease who were diagnosed of brucellosis during the period from 2011 to 2016 was performed. Diagnostic criteria, clinical presentations, and outcomes were recorded. The most common symptom was fever. Osteoarticular involvement was found in 50% of the patients. They were infected by contacting with infected animals or consuming of unpasteurized milk or meat of sheep or goats, also...
September 19, 2017: Clinical Rheumatology
https://www.readbyqxmd.com/read/28923082/characterization-of-a-secreted-cystatin-of-the-parasitic-nematode-haemonchus-contortus-and-its-immune-modulatory-effect-on-goat-monocytes
#18
Yujian Wang, Lingyan Wu, Xinchao Liu, Shuai Wang, Muhammad Ehsan, RuoFeng Yan, XiaoKai Song, LiXin Xu, XiangRui Li
BACKGROUND: Haemonchosis is a disease of the small ruminant caused by a nematode parasite Haemonchus contortus, and it is most important and alarming challenges to the small ruminant's production. The infection of the H. contortus could cause high economic losses worldwide. H. contortus is a blood feeding parasite which penetrates into the abomasal mucosa to feed the blood of the host and causing the anemia and decreased total plasma protein. Modulation and suppression of the immune response of the host by nematode parasites have been reported extensively, and the cysteine protease inhibitor (cystatin) is identified as one of the major immunomodulators...
September 18, 2017: Parasites & Vectors
https://www.readbyqxmd.com/read/28922262/sugar-in-infants-children-and-adolescents-a-position-paper-of-the-european-society-for-paediatric-gastroenterology-hepatology-and-nutrition-committee-on-nutrition
#19
Nataša Fidler Mis, Christian Braegger, Jiri Bronsky, Cristina Campoy, Magnus Domellöf, Nicholas D Embleton, Iva Hojsak, Jessie Hulst, Flavia Indrio, Alexandre Lapillonne, Walter Mihatsch, Christian Molgaard, Rakesh Vora, Mary Fewtrell
The consumption of sugars, particularly sugar-sweetened beverages (SSBs; beverages or drinks that contain added caloric sweeteners (i.e. sucrose, high-fructose corn syrup, fruit-juice concentrates), in European children and adolescents exceeds current recommendations. This is of concern because there is no nutritional requirement for free sugars, and infants have an innate preference for sweet taste, which may be modified and reinforced by pre- and postnatal exposures. Sugar containing beverages/free sugars increase the risk for overweight/obesity and dental caries, can result in poor nutrient supply and reduced dietary diversity and may be associated with increased risk of type 2 diabetes mellitus, cardiovascular risk, and other health effects...
September 16, 2017: Journal of Pediatric Gastroenterology and Nutrition
https://www.readbyqxmd.com/read/28920418/myogenic-satellite-cells-differentiation-with-linolenic-and-retinoic-and-thiazolidenediones-from-prepubertal-korean-black-goat
#20
Sugathan Subi, Sungjin Lee, Supriya Shiwani, Naresh Kumar Singh
Objective: Myogenic satellite cells were isolated from semitendinosus muscle of prepubertal Korean black goat to observe the differential effect of linolenic and retinoic acid in thepresence of thiazolidinediones and also to observe the production insulin sensitive preadipocyte. Methods: Cells were characterized for its stemness with CD 34, CD 13, CD 106, CD44, Vimentin surface markers using flow cytometry. Cells characterized themselves to possess significant (p<0...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
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