keyword
MENU ▼
Read by QxMD icon Read
search

Camelid

keyword
https://www.readbyqxmd.com/read/29324815/biophysical-insights-from-a-single-chain-camelid-antibody-directed-against-the-disrupted-in-schizophrenia-1-protein
#1
Antony S K Yerabham, Andreas Müller-Schiffmann, Tamar Ziehm, Andreas Stadler, Sabrina Köber, Xela Indurkhya, Rita Marreiros, Svenja V Trossbach, Nicholas J Bradshaw, Ingrid Prikulis, Dieter Willbold, Oliver H Weiergräber, Carsten Korth
Accumulating evidence suggests an important role for the Disrupted-in-Schizophrenia 1 (DISC1) protein in neurodevelopment and chronic mental illness. In particular, the C-terminal 300 amino acids of DISC1 have been found to mediate important protein-protein interactions and to harbor functionally important phosphorylation sites and disease-associated polymorphisms. However, long disordered regions and oligomer-forming subdomains have so far impeded structural analysis. VHH domains derived from camelid heavy chain only antibodies are minimal antigen binding modules with appreciable solubility and stability, which makes them well suited for the stabilizing proteins prior to structural investigation...
2018: PloS One
https://www.readbyqxmd.com/read/29322100/bglbrick-strategy-for-the-construction-of-single-domain-antibody-fusions
#2
Ellen R Goldman, Aeris Broussard, George P Anderson, Jinny L Liu
Single domain antibodies, recombinantly expressed variable domains derived from camelid heavy chain antibodies, are often expressed as multimers for detection and therapeutic applications. Constructs in which several single domain antibodies are genetically fused serially, as well as those in which single domain antibodies are genetically linked with domains that naturally form multimers, yield improvement in apparent binding affinity due to avidity. Here, using a single domain antibody that binds envelope protein from the Dengue virus, we demonstrated the construction of single domain antibody dimers using the Bglbrick cloning strategy...
December 2017: Heliyon
https://www.readbyqxmd.com/read/29297802/prevalence-and-molecular-identification-of-piroplasmids-in-iranian-dromedaries-camelus-dromedarius
#3
Somayeh Bahrami, Mohammad Reza Tabandeh, Ali Reza Ganjali Tafreshi
Camels ( Camelus dromedarius) are important, multipurpose local animals in Iran. Despite their importance, camelid parasitic diseases have not received adequate attention in the veterinary literature. The present study investigated the prevalence of, and molecularly identified, camel piroplasms in Iran. Blood samples from 248 camels from five different regions of Iran were screened for the presence of piroplasmid infection using an 18SrRNA polymerase chain reaction (PCR) sequencing method. Of the 248 samples, 16 were positive for piroplasms via PCR (6...
December 2017: Journal of Zoo and Wildlife Medicine: Official Publication of the American Association of Zoo Veterinarians
https://www.readbyqxmd.com/read/29276097/abomasal-and-third-compartment-ulcers-in-ruminants-and-south-american-camelids
#4
REVIEW
Alexandra Hund, Thomas Wittek
Abomasal ulcers are frequent incidental findings in necropsies of domestic ruminants and South American Camelids (SAC) or in slaughter animals and are a frequent cause of death in the most affected group of cattle, veal calves. Their true prevalence and significance is unknown owing to limitations in diagnosing the condition in live animals. This article discusses types of ulcers, possible causes of ulceration, and the clinical consequences, symptoms, and differential diagnoses, as well as further diagnostics in cattle, small ruminants and SAC...
December 21, 2017: Veterinary Clinics of North America. Food Animal Practice
https://www.readbyqxmd.com/read/29249328/monitoring-and-controlling-follicular-activity-in-camelids
#5
Ahmed Tibary
This paper reviews that state of our knowledge concerning follicular wave dynamics, monitoring and manipulation. All camelids have overlapping follicular waves in absence of ovulation which is induced by a seminal plasma factor (βNGF). The interval between follicular waves varies. The size of the ovulatory follicle varies between 11 and 25 mm in camels and between in 6 and 13 mm in South American Camelids. The interval between induction of ovulation and next ovulatory follicle is 15 ± 1 day for all camelids...
December 8, 2017: Theriogenology
https://www.readbyqxmd.com/read/29233140/selection-characterization-and-thermal-stabilization-of-llama-single-domain-antibodies-towards-ebola-virus-glycoprotein
#6
Jinny L Liu, Lisa C Shriver-Lake, George P Anderson, Dan Zabetakis, Ellen R Goldman
BACKGROUND: A key advantage of recombinant antibody technology is the ability to optimize and tailor reagents. Single domain antibodies (sdAbs), the recombinantly produced variable domains derived from camelid and shark heavy chain antibodies, provide advantages of stability and solubility and can be further engineered to enhance their properties. In this study, we generated sdAbs specific for Ebola virus envelope glycoprotein (GP) and increased their stability to expand their utility for use in austere locals...
December 12, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/29228062/market-access-and-community-size-influence-pastoral-management-of-native-and-exotic-livestock-species-a-case-study-in-communities-of-the-cordillera-real-in-bolivia-s-high-andean-wetlands
#7
Quentin Struelens, Karina Gonzales Pomar, Susi Loza Herrera, Gaby Nina Huanca, Olivier Dangles, François Rebaudo
Grazing areas management is of utmost importance in the Andean region. In the valleys of the Bolivian Cordillera Real near La Paz, pastoralism constitutes the traditional way for people to insure food security and economical sustainability. In these harsh mountains, unique and productive wetlands sustained by glacial water streams are of utmost importance for feeding cattle herds during the dry season. After the colonization by the Spanish, a shift in livestock species has been observed, with the introduction of exotic species such as cows and sheep, resulting in a different impact on pastures compared to native camelid species-llamas and alpacas...
2017: PloS One
https://www.readbyqxmd.com/read/29213270/nanobodies-and-nanobody-based-human-heavy-chain-antibodies-as-antitumor-therapeutics
#8
REVIEW
Peter Bannas, Julia Hambach, Friedrich Koch-Nolte
Monoclonal antibodies have revolutionized cancer therapy. However, delivery to tumor cells in vivo is hampered by the large size (150 kDa) of conventional antibodies. The minimal target recognition module of a conventional antibody is composed of two non-covalently associated variable domains (VH and VL). The proper orientation of these domains is mediated by their hydrophobic interface and is stabilized by their linkage to disulfide-linked constant domains (CH1 and CL). VH and VL domains can be fused via a genetic linker into a single-chain variable fragment (scFv)...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29209322/camelid-single-domain-antibodies-historical-perspective-and-future-outlook
#9
Mehdi Arbabi-Ghahroudi
Tremendous effort has been expended over the past two and a half decades to understand many aspects of camelid heavy chain antibodies, from their biology, evolution, and immunogenetics to their potential applications in various fields of research and medicine. In this article, I present a historical perspective on the development of camelid single-domain antibodies (sdAbs or VHHs, also widely known as nanobodies) since their discovery and discuss the advantages and disadvantages of these unique molecules in various areas of research, industry, and medicine...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29207143/screening-and-antitumor-effect-of-an-anti%C3%A2-ctla%C3%A2-4-nanobody
#10
Ruirong Wan, Aiqun Liu, Xiaoqiong Hou, Zongqiang Lai, Jieping Li, Nuo Yang, Juntao Tan, Fengzhen Mo, Zixi Hu, Xiaomei Yang, Yongxiang Zhao, Xiaoling Lu
Cytotoxic T‑lymphocyte antigen‑4 (CTLA‑4) is a critical negative regulator of immune responses. CTLA‑4 is rapidly upregulated following T‑cell activation, and then binds to B7 molecules with a higher affinity than CD28. CTLA‑4 may abolish the initiation of the responses of T cells by raising the threshold of signals required for full activation of T cells, and it also may terminate ongoing T-cell responses. This regulatory role has led to the development of monoclonal antibodies (mAbs) designed to block CTLA‑4 activity for enhancing immune responses against cancer...
December 4, 2017: Oncology Reports
https://www.readbyqxmd.com/read/29182399/application-of-camelid-heavy-chain-variable-domains-vhhs-in-prevention-and-treatment-of-bacterial-and-viral-infections
#11
Lucas Wilken, Anne McPherson
Camelid heavy-chain variable domains (VHHs) are the smallest, intact, antigen-binding units to occur in nature. VHHs possess high degrees of solubility and robustness enabling generation of multivalent constructs with increased avidity - characteristics that mark their superiority to other antibody fragments and monoclonal antibodies. Capable of effectively binding to molecular targets inaccessible to classical immunotherapeutic agents and easily produced in microbial culture, VHHs are considered promising tools for pharmaceutical biotechnology...
November 28, 2017: International Reviews of Immunology
https://www.readbyqxmd.com/read/29181380/production-preservation-and-transfer-of-south-american-camelid-embryos
#12
REVIEW
Virginia L Trasorras, María Ignacia Carretero, Deborah M Neild, Maria Graciela Chaves, Susana M Giuliano, Marcelo H Miragaya
The current review summarizes progress in the field of in vitro and in vivo production of South American Camelid embryos. Both methods require ovarian superstimulation (with FSH and eCG) to obtain multiple ovulations (in vivo embryo production) or to induce follicle growth for oocyte collection (in vitro embryo production). Moreover, superstimulation entails prior administration of hormones that inhibit follicular growth (progesterone, progestagens, and estrogens). Cumulus-oocyte complexes obtained must mature in vivo (buserelin administration) or in vitro to then be subjected to in vitro fertilization or intracytoplasmic sperm injection...
2017: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29177623/expression-of-single-domain-antibody-in-different-systems
#13
REVIEW
Yongkang Liu, He Huang
Camelid single-domain antibodies (sdAbs, VHHs, or Nanobodies®) are types of antibody fragments that are composed of the heavy-chain variable domain only. These VHHs possess unique structural and functional features, as they are small in size and exhibit thermal stability and high solubility. Compared to conventional antibodies, VHHs can be manufactured in microorganisms to significantly save on cost, labor, and time since VHHs lack the Fc domain with its N-linked oligosaccharide. Until now, VHHs have been expressed in several kinds of production systems, ranging from prokaryotic cells, yeasts, fungi, insect cells, and mammalian cell lines, to plants...
November 25, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29163485/a-novel-affinity-tag-abtag-and-its-application-to-the-affinity-screening-of-single-domain-antibodies-selected-by-phage-display
#14
Greg Hussack, Toya Nath Baral, Jason Baardsnes, Henk van Faassen, Shalini Raphael, Kevin A Henry, Jianbing Zhang, C Roger MacKenzie
ABTAG is a camelid single-domain antibody (sdAb) that binds to bovine serum albumin (BSA) with low picomolar affinity. In surface plasmon resonance (SPR) analyses using BSA surfaces, bound ABTAG can be completely dissociated from the BSA surfaces at low pH, over multiple cycles, without any reduction in the capacity of the BSA surfaces to bind ABTAG. A moderate throughput, SPR-based, antibody screening assay exploiting the unique features of ABTAG is described. Anti-carcinoembryonic antigen-related cell adhesion molecule 6 (CEACAM6) sdAbs were isolated from a phage-displayed sdAb library derived from the heavy chain antibody repertoire of a llama immunized with CEACAM6...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/29156539/epidemiological-investigations-of-four-cowpox-virus-outbreaks-in-alpaca-herds-germany
#15
Almut Prkno, Donata Hoffmann, Daniela Goerigk, Matthias Kaiser, Anne Catherine Franscisca van Maanen, Kathrin Jeske, Maria Jenckel, Florian Pfaff, Thomas W Vahlenkamp, Martin Beer, Rainer G Ulrich, Alexander Starke, Martin Pfeffer
Four cowpox virus (CPXV) outbreaks occurred in unrelated alpaca herds in Eastern Germany during 2012-2017. All incidents were initially noticed due to severe, generalized, and finally lethal CPXV infections, which were confirmed by testing of tissue and serum samples. As CPXV-infection has been described in South American camelids (SACs) only three times, all four herds were investigated to gain a deeper understanding of CPXV epidemiology in alpacas. The different herds were investigated twice, and various samples (serum, swab samples, and crusts of suspicious pox lesions, feces) were taken to identify additionally infected animals...
November 18, 2017: Viruses
https://www.readbyqxmd.com/read/29123178/a-nanobody-based-tracer-targeting-dpp6-for-non-invasive-imaging-of-human-pancreatic-endocrine-cells
#16
Alexander Balhuizen, Sam Massa, Iris Mathijs, Jean-Valery Turatsinze, Jens De Vos, Stéphane Demine, Catarina Xavier, Olatz Villate, Isabelle Millard, Dominique Egrise, Carmen Capito, Raphaël Scharfmann, Pieter In't Veld, Piero Marchetti, Serge Muyldermans, Serge Goldman, Tony Lahoutte, Luc Bouwens, Decio L Eizirik, Nick Devoogdt
There are presently no reliable ways to quantify endocrine cell mass (ECM) in vivo, which prevents an accurate understanding of the progressive beta cell loss in diabetes or following islet transplantation. To address this unmet need, we coupled RNA sequencing of human pancreatic islets to a systems biology approach to identify new biomarkers of the endocrine pancreas. Dipeptidyl-Peptidase 6 (DPP6) was identified as a target whose mRNA expression is at least 25-fold higher in human pancreatic islets as compared to surrounding tissues and is not changed by proinflammatory cytokines...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29116505/construction-of-high-quality-camel-immune-antibody-libraries
#17
Ema Romão, Vianney Poignavent, Cécile Vincke, Christophe Ritzenthaler, Serge Muyldermans, Baptiste Monsion
Single-domain antibodies libraries of heavy-chain only immunoglobulins from camelids or shark are enriched for high-affinity antigen-specific binders by a short in vivo immunization. Thus, potent binders are readily retrieved from relatively small-sized libraries of 10(7)-10(8) individual transformants, mostly after phage display and panning on a purified target. However, the remaining drawback of this strategy arises from the need to generate a dedicated library, for nearly every envisaged target. Therefore, all the procedures that shorten and facilitate the construction of an immune library of best possible quality are definitely a step forward...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29110898/successful-pregnancies-from-vitrified-embryos-in-the-dromedary-camel-avoidance-of-a-possible-toxic-effect-of-sucrose-on-embryos
#18
M Herrid, M Billah, J A Skidmore
Successful embryo cryopreservation facilitates the wider application of assisted reproduction technologies and also provides a useful method for gene banking of valuable genetics. Unfortunately attempts to establish an effective cryopreservation protocol for camelid embryos have been unsuccessful. In the current study, a modified vitrification protocol with three steps was investigated, whereby embryos were exposed to solutions containing increasing amounts of glycerol and ethylene glycol for fixed time periods...
October 26, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/29054987/theranostic-radiolabeled-anti-cd20-sdab-for-targeted-radionuclide-therapy-of-non-hodgkin-lymphoma
#19
Ahmet Krasniqi, Matthias D'Huyvetter, Catarina Xavier, Kevin Van der Jeught, Serge Muyldermans, José Van Der Heyden, Tony Lahoutte, Jan Tavernier, Nick Devoogdt
Anti-CD20 radioimmunotherapy is an effective approach for therapy of relapsed or refractory CD20pos lymphomas, but faces limitations due to poor tumor penetration and undesirable pharmacokinetics of full antibodies. Camelid single-domain Ab fragments (sdAbs) might circumvent some of the limitations of radiolabeled full antibodies. In this study, a set of hCD20-targeting sdAbs was generated and their capacity to bind hCD20 was evaluated in vitro and in vivo. A lead sdAb, sdAb 9079, was selected based on its specific tumor targeting and significant lower kidney accumulation compared to other sdAbs...
October 20, 2017: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29045695/a-new-collection-of-amblyomma-parvitarsum-acari-ixodidae-in-peru-with-description-of-a-gynandromorph-and-report-of-rickettsia-detection
#20
S Muñoz-Leal, T F Martins, L R Luna, A Rodriguez, M B Labruna
Adult stages of Amblyomma parvitarsum Neumann parasitize wild and domestic camelids of the genera Lama and Vicugna in highlands of Andean Plateau and Patagonia. Within the Peruvian Andes, few reports have documented this tick-host association, and although reported in Chile and Argentina, Rickettsia-infected A. parvitarsum remains undocumented for this country. Here we report a new collection of A. parvitarsum from Peru, the finding of the first gynandromorph for the species and high prevalence of Rickettsia in adult stages...
October 17, 2017: Journal of Medical Entomology
keyword
keyword
18954
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"