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Lung cancer metabolomics

A Blomme, B Costanza, P de Tullio, M Thiry, G Van Simaeys, S Boutry, G Doumont, E Di Valentin, T Hirano, T Yokobori, S Gofflot, O Peulen, A Bellahcène, F Sherer, C Le Goff, E Cavalier, A Mouithys-Mickalad, F Jouret, P G Cusumano, E Lifrange, R N Muller, S Goldman, P Delvenne, E De Pauw, M Nishiyama, V Castronovo, A Turtoi
Myoferlin is a multiple C2-domain-containing protein that regulates membrane repair, tyrosine kinase receptor function and endocytosis in myoblasts and endothelial cells. Recently it has been reported as overexpressed in several cancers and shown to contribute to proliferation, migration and invasion of cancer cells. We have previously demonstrated that myoferlin regulates epidermal growth factor receptor activity in breast cancer. In the current study, we report a consistent overexpression of myoferlin in triple-negative breast cancer cells (TNBC) over cells originating from other breast cancer subtypes...
October 24, 2016: Oncogene
Jiaqi Huang, Alison M Mondul, Stephanie J Weinstein, Stella Koutros, Andriy Derkach, Edward Karoly, Joshua N Sampson, Steven C Moore, Sonja I Berndt, Demetrius Albanes
BACKGROUND: Two recent metabolomic analyses found serum lipid, energy, and other metabolites related to aggressive prostate cancer risk up to 20 years prior to diagnosis. METHODS: We conducted a serum metabolomic investigation of prostate cancer risk in the Prostate, Lung, Colorectal, and Ovarian Cancer Screening Trial that included annual serum total prostate-specific antigen measurement and digital rectal examination. This nested study included 380 cases diagnosed post-screening and 380 controls individually matched to cases on age, race, study centre, and blood-collection date (median time to diagnosis, 10 years (range 4...
September 27, 2016: British Journal of Cancer
Sander Bins, Geert A Cirkel, Christa G Gadellaa-van Hooijdonk, Fleur Weeber, Isaac J Nijman, Annette H Bruggink, Paul J van Diest, Stefan M Willems, Wouter B Veldhuis, Michel M van den Heuvel, Rob J de Knegt, Marco J Koudijs, Erik van Werkhoven, Ron H J Mathijssen, Edwin Cuppen, Stefan Sleijfer, Jan H M Schellens, Emile E Voest, Marlies H G Langenberg, Maja J A de Jonge, Neeltje Steeghs, Martijn P Lolkema
BACKGROUND: The discovery of novel biomarkers that predict treatment response in advanced cancer patients requires acquisition of high-quality tumor samples. As cancer evolves over time, tissue is ideally obtained before the start of each treatment. Preferably, samples are freshly frozen to allow analysis by next-generation DNA/RNA sequencing (NGS) but also for making other emerging systematic techniques such as proteomics and metabolomics possible. Here, we describe the first 469 image-guided biopsies collected in a large collaboration in The Netherlands (Center for Personalized Cancer Treatment) and show the utility of these specimens for NGS analysis...
September 23, 2016: Oncologist
Xiaoli Zhang, Xinyue Zhu, Caihong Wang, Haixia Zhang, Zhiming Cai
Lung cancer is the most common cause of cancer death in China. We characterized metabolic alterations in lung cancer using two analytical platforms: a non-targeted metabolic profiling strategy based on proton nuclear magnetic resonance (1H-NMR) spectroscopy and a targeted metabolic profiling strategy based on rapid resolution liquid chromatography (RRLC). Changes in serum metabolite levels during oncogenesis were evaluated in 25 stage I lung cancer patients and matched healthy controls. We identified 25 metabolites that were differentially regulated between the lung cancer patients and matched controls...
August 23, 2016: Oncotarget
Teresa W-M Fan, Marc O Warmoes, Qiushi Sun, Huan Song, Jadwiga Turchan-Cholewo, Jeremiah T Martin, Angela Mahan, Richard M Higashi, Andrew N Lane
Cancer and stromal cell metabolism is important for understanding tumor development, which highly depends on the tumor microenvironment (TME). Cell or animal models cannot recapitulate the human TME. We have developed an ex vivo paired cancerous (CA) and noncancerous (NC) human lung tissue approach to explore cancer and stromal cell metabolism in the native human TME. This approach enabled full control of experimental parameters and acquisition of individual patient's target tissue response to therapeutic agents while eliminating interferences from genetic and physiological variations...
July 2016: Cold Spring Harbor Molecular Case Studies
Aryo D Pamungkas, Changyoung Park, Sungyong Lee, Sun Ha Jee, Youngja H Park
BACKGROUND: The cancer death rate escalated during 20th century. In South Korea, lung cancer is expected to contribute 12,736 deaths in men, the highest amount among all cancers. Several risk factors may increase the chance to acquiring lung cancer, with mostly related to exogenous compounds found in cigarette smoke and synthetic manufacturing materials. As the mortality rate of lung cancer increases, deeper understanding is necessary to explore risk factors that may lead to this malignancy...
2016: Respiratory Research
Andrew N Lane, Richard M Higashi, Teresa W-M Fan
AIMS: In this review we compare the advantages and disadvantages of different model biological systems for determining the metabolic functions of cells in complex environments, how they may change in different disease states, and respond to therapeutic interventions. BACKGROUND: All preclinical drug-testing models have advantages and drawbacks. We compare and contrast established cell, organoid and animal models with ex vivo organ or tissue culture and in vivo human experiments in the context of metabolic readout of drug efficacy...
July 2016: Metabolomics: Official Journal of the Metabolomic Society
Keiron O'Shea, Simon J S Cameron, Keir E Lewis, Chuan Lu, Luis A J Mur
BACKGROUND: Lung cancer (LC) is one of the leading lethal cancers worldwide, with an estimated 18.4% of all cancer deaths being attributed to the disease. Despite developments in cancer diagnosis and treatment over the previous thirty years, LC has seen little to no improvement in the overall five year survival rate after initial diagnosis. METHODS: In this paper, we extended a recent study which profiled the metabolites in sputum from patients with lung cancer and age-matched volunteers smoking controls using flow infusion electrospray ion mass spectrometry...
November 2016: Biochimica et Biophysica Acta
Silvia Veneroni, Matteo Dugo, Maria Grazia Daidone, Egidio Iorio, Barbara Valeri, Patrizia Pinciroli, Maida De Bortoli, Edoardo Marchesi, Patrizia Miodini, Elena Taverna, Alessandro Ricci, Silvana Canevari, Giuseppe Pelosi, Italia Bongarzone
CONTEXT: Biobanks of frozen human normal and malignant tissues represent a valuable source for "omics" analysis in translational cancer research and molecular pathology. However, the success of molecular and cellular analysis strongly relies on the collection, handling, storage procedures, and quality control of fresh human tissue samples. OBJECTIVE: We tested whether under vacuum storage (UVS) effectively preserves tissues during the time between surgery and storage for "omics" analyses...
July 12, 2016: Biopreservation and Biobanking
Ping-Ching Hsu, Renny S Lan, Theodore M Brasky, Catalin Marian, Amrita K Cheema, Habtom W Ressom, Christopher A Loffredo, Wallace B Pickworth, Peter G Shields
Smoking-related biomarkers for lung cancer and other diseases are needed to enhance early detection strategies and to provide a science base for tobacco product regulation. An untargeted metabolomics approach by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOF MS) totaling 957 assays was used in a novel experimental design where 105 current smokers smoked 2 cigarettes one hour apart. Blood was collected immediately before and after each cigarette allowing for within-subject replication...
June 24, 2016: Molecular Carcinogenesis
Fengwei Tan, Ning Li, Shugeng Gao, Jie He
Precision medicine is to developing the most appropriate individualized treatment for each patient based on the macro to the micro level of individual differences. Genomic, proteomics, metabolomics data, and other big data analysis methods are the essence of precision medicine. Precision medicine brings the hope to overcome cancer. Among all kinds of tumors, lung cancer is the biggest threat to human. This paper reviewed the development of precision medicine in the surgical treatment of lung cancer.
June 20, 2016: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
Vered Marks, Anisleidys Munoz, Priyamvada Rai, Jamie D Walls
Metabolomic profiling is an increasingly important method for identifying potential biomarkers in cancer cells with a view towards improved diagnosis and treatment. Nuclear magnetic resonance (NMR) provides a potentially noninvasive means to accurately characterize differences in the metabolomic profiles of cells. In this work, we use (1)H NMR to measure the metabolomic profiles of water soluble metabolites extracted from isogenic control and oncogenic HRAS-, KRAS-, and NRAS-transduced BEAS2B lung epithelial cells to determine the robustness of NMR metabolomic profiling in detecting differences between the transformed cells and their untransformed counterparts as well as differences among the RAS-transformed cells...
2016: PeerJ
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June 2016: Annals of Intensive Care
Paloma Navarro, Maria J Bueno, Ivana Zagorac, Tamara Mondejar, Jesus Sanchez, Silvana Mourón, Javier Muñoz, Gonzalo Gómez-López, Veronica Jimenez-Renard, Francisca Mulero, Navdeep S Chandel, Miguel Quintela-Fandino
Epithelial malignancies are effectively treated by antiangiogenics; however, acquired resistance is a major problem in cancer therapeutics. Epithelial tumors commonly have mutations in the MAPK/Pi3K-AKT pathways, which leads to high-rate aerobic glycolysis. Here, we show how multikinase inhibitor antiangiogenics (TKIs) induce hypoxia correction in spontaneous breast and lung tumor models. When this happens, the tumors downregulate glycolysis and switch to long-term reliance on mitochondrial respiration. A transcriptomic, metabolomic, and phosphoproteomic study revealed that this metabolic switch is mediated by downregulation of HIF1α and AKT and upregulation of AMPK, allowing uptake and degradation of fatty acids and ketone bodies...
June 21, 2016: Cell Reports
Elisa Caiola, Laura Brunelli, Mirko Marabese, Massimo Broggini, Monica Lupi, Roberta Pastorelli
KRAS mutations in non-small-cell lung cancer (NSCLC) patients are considered a negative predictive factor and indicate poor response to anticancer treatments. KRAS mutations lead to activation of the PI3K/akt/mTOR pathway, whose inhibition remains a challenging clinical target. Since the PI3K/akt/mTOR pathway and KRAS oncogene mutations all have roles in cancer cell metabolism, we investigated whether the activity of PI3K/akt/mTOR inhibitors (BEZ235 and BKM120) in cells harboring different KRAS status is related to their metabolic effect...
June 6, 2016: Oncotarget
Belén Callejón-Leblic, Tamara García-Barrera, Jesús Grávalos-Guzmán, Antonio Pereira-Vega, José Luis Gómez-Ariza
UNLABELLED: Lung cancer is one of the ten most common causes of death worldwide, so that the search for early diagnosis biomarkers is a very challenging task. Bronchoalveolar lavage fluid (BALF) provides information on cellular and biochemical epithelial surface of the lower respiratory tract constituents and no previous metabolomic studies have been performed with BALF samples from patients with lung cancer. Therefore, this fluid has been explored looking for new contributions in lung cancer metabolism...
August 11, 2016: Journal of Proteomics
Selma Masri, Thales Papagiannakopoulos, Kenichiro Kinouchi, Yu Liu, Marlene Cervantes, Pierre Baldi, Tyler Jacks, Paolo Sassone-Corsi
The circadian clock controls metabolic and physiological processes through finely tuned molecular mechanisms. The clock is remarkably plastic and adapts to exogenous "zeitgebers," such as light and nutrition. How a pathological condition in a given tissue influences systemic circadian homeostasis in other tissues remains an unanswered question of conceptual and biomedical importance. Here, we show that lung adenocarcinoma operates as an endogenous reorganizer of circadian metabolism. High-throughput transcriptomics and metabolomics revealed unique signatures of transcripts and metabolites cycling exclusively in livers of tumor-bearing mice...
May 5, 2016: Cell
Junjun Ni, Li Xu, Wei Li, Lijun Wu
Easy-to-use early cancer detection methods based on metabolomics using serum samples have been developed recently. Among metabolites, amino acids and acylcarnitine are two of the most suitable candidates for diagnosing lung cancer. The purpose of the present study was to develop a novel, sensitive and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to simultaneously determine thirteen amino acids and eight acylcarnitines in lung cancer patients in serum. After derivatization, the 21 analytes were separated by a C18 column with gradient elution program in 14 minutes, obtaining recovery within 90...
April 30, 2016: Biomedical Chromatography: BMC
Daniel Weindl, Thekla Cordes, Nadia Battello, Sean C Sapcariu, Xiangyi Dong, Andre Wegner, Karsten Hiller
BACKGROUND: Metabolism gained increasing interest for the understanding of diseases and to pinpoint therapeutic intervention points. However, classical metabolomics techniques only provide a very static view on metabolism. Metabolic flux analysis methods, on the other hand, are highly targeted and require detailed knowledge on metabolism beforehand. RESULTS: We present a novel workflow to analyze non-targeted metabolome-wide stable isotope labeling data to detect metabolic flux changes in a non-targeted manner...
2016: Cancer & Metabolism
Desirée Hao, M Omair Sarfaraz, Farshad Farshidfar, D Gwyn Bebb, Camelia Y Lee, Cynthia M Card, Marilyn David, Aalim M Weljie
Lung cancer causes more deaths in men and women than any other cancer related disease. Currently, few effective strategies exist to predict how patients will respond to treatment. We evaluated the serum metabolomic profiles of 25 lung cancer patients undergoing chemotherapy ± radiation to evaluate the feasibility of metabolites as temporal biomarkers of clinical outcomes. Serial serum specimens collected prospectively from lung cancer patients were analyzed using both nuclear magnetic resonance ((1)H-NMR) spectroscopy and gas chromatography mass spectrometry (GC-MS)...
2016: Metabolomics: Official Journal of the Metabolomic Society
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