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https://www.readbyqxmd.com/read/28531660/carbon-black-aggregates-cause-endothelial-dysfunction-by-activating-rock
#1
Junyan Yan, Chia-Hsiang Lai, Shih-Chun Candice Lung, Wen-Cheng Wang, Chih-Ching Huang, Guan-Wen Chen, Guangli Suo, Cheng-Tai Choug, Chia-Hua Lin
Carbon black nanoparticles (CBNs) have been associated with the progression of atherosclerosis. CBNs normally enter the bloodstream and crosslink together to form agglomerates. However, most studies have used nano-sized CB particles to clarify the involvement of CBN exposure in CBN-induced endothelial dysfunction. Herein, we studied endothelial toxicity of CBN aggregates (CBA) to human EA.hy926 vascular cells. Cell viability, lactate dehydrogenase leakage, and oxidative stress were affected by the highest concentration of CBA...
May 15, 2017: Journal of Hazardous Materials
https://www.readbyqxmd.com/read/28531196/co-inheritance-of-glucose-6-phosphate-dehydrogenase-deficiency-mutations-and-hemoglobin-e-in-a-kachin-population-in-a-malaria-endemic-region-of-southeast-asia
#2
Zeshuai Deng, Fang Yang, Yao Bai, Lijun He, Qing Li, Yanrui Wu, Lan Luo, Hong Li, Limei Ma, Zhaoqing Yang, Yongshu He, Liwang Cui
Glucose-6-phosphate dehydrogenase (G6PD) deficiency and hemoglobin E (HbE, β26 Glu-Lys) are two common red cell disorders in Southeast Asia. G6PD deficiency produces hemolytic anemia, which can be triggered by certain drugs or infections. HbE is asymptomatic or is manifested as microcytic, minimally hemolytic anemia. The association between G6PD deficiency and HbE is little understood. This study aimed to investigate G6PD deficiency and HbE in a Kachin ethnic group in the China-Myanmar border area. G6PD enzyme activity was measured using a quantitative G6PD assay, G6PD variants genotyped by the SNaPshot assay, and an HbE gene mutation identified by an amplification refractory mutation system and subsequently confirmed by using a reverse dot blot hybridization assay from 100 unrelated individuals in the study area...
2017: PloS One
https://www.readbyqxmd.com/read/28530221/micu1-drives-glycolysis-and-chemoresistance-in-ovarian-cancer
#3
Prabir K Chakraborty, Soumyajit Banerjee Mustafi, Xunhao Xiong, Shailendra Kumar Dhar Dwivedi, Vasyl Nesin, Sounik Saha, Min Zhang, Danny Dhanasekaran, Muralidharan Jayaraman, Robert Mannel, Kathleen Moore, Scott McMeekin, Da Yang, Rosemary Zuna, Kai Ding, Leonidas Tsiokas, Resham Bhattacharya, Priyabrata Mukherjee
Cancer cells actively promote aerobic glycolysis to sustain their metabolic requirements through mechanisms not always clear. Here, we demonstrate that the gatekeeper of mitochondrial Ca(2+) uptake, Mitochondrial Calcium Uptake 1 (MICU1/CBARA1) drives aerobic glycolysis in ovarian cancer. We show that MICU1 is overexpressed in a panel of ovarian cancer cell lines and that MICU1 overexpression correlates with poor overall survival (OS). Silencing MICU1 in vitro increases oxygen consumption, decreases lactate production, inhibits clonal growth, migration and invasion of ovarian cancer cells, whereas silencing in vivo inhibits tumour growth, increases cisplatin efficacy and OS...
May 22, 2017: Nature Communications
https://www.readbyqxmd.com/read/28530096/engineering-eschericha-coli-for-enhanced-tyrosol-production
#4
Yuxiang Xue, Xianzhong Chen, Cui Yang, Junzhuang Chang, Wei Shen, You Fan
Tyrosol is a phenolic compound found in olive oil and wines. The health benefits of tyrosol have attracted considerable attention. Because the tyrosol extraction from plants poses a major obstacle, biosynthesizing this compound using microbial hosts is of interest. In this study, the phenylpyruvate decarboxylase gene ARO10 and the aromatic amino acid aminotransferase gene ARO8 were introduced into Escherichia coli to generate two recombinant tyrosol producers. Deleting the prephenate dehydratase pheA and the phenylacetaldehyde dehydrogenase gene feaB improved the tyrosol production...
May 22, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28529818/high-protein-fish-oil-diet-prevents-hepatic-steatosis-in-noncnzo10-mice-association-with-diet-genetics-regulated-micro-rnas
#5
Nikhil Adi, Jennipher Adi, Roberta Marques Lassance-Soares, Paul Kurlansky, Hong Yu, Keith A Webster
OBJECTIVE: NONcNZO10 (NZ10) mice are predisposed to obesity and develop type 2 diabetes (T2D) and hepatic steatosis even when maintained on a control diet (CD) of 6% fat. Studies were designed to determine whether this extreme susceptibility phenotype could be alleviated by diet and if so the molecular targets of diet. METHODS: NZ10 and SWR/J (SWR) control mice were fed a CD or a test diet of high protein and fish oil (HPO) for 19 weeks and then analyzed for steatosis, blood chemistry, hepatic gene and micro-RNA expression...
June 2016: Journal of Diabetes & Metabolism
https://www.readbyqxmd.com/read/28529598/biological-interaction-of-cigarette-smoking-on-the-association-between-genetic-polymorphisms-involved-in-inflammation-and-the-risk-of-lung-cancer-a-case-control-study-in-japan
#6
Yuzo Yamamoto, Chikako Kiyohara, Saiko Suetsugu-Ogata, Naoki Hamada, Yoichi Nakanishi
Chronic inflammation serves an important role in lung carcinogenesis, thus genetic polymorphisms involved in this pathway may affect the risk of lung cancer. The present case-control study focused on the association between lung cancer risk and genetic polymorphisms involved in inflammatory pathways. The study comprised 462 lung cancer cases and 379 controls from Japan. The roles of interleukin 8 (IL8) rs4073, nuclear factor kappa B (NFκB) rs28362491, cytochrome b-245, alpha polypeptide (CYBA) rs4673, NAD(P) H dehydrogenase, quinone 1 (NQO1) rs1800566, nitric oxide synthase 2 and inducible (NOS2) rs2297518 polymorphisms in lung carcinogenesis were investigated...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28529047/bezafibrate-prevents-mitochondrial-dysfunction-antioxidant-system-disturbance-glial-reactivity-and-neuronal-damage-induced-by-sulfite-administration-in-striatum-of-rats-implications-for-a-possible-therapeutic-strategy-for-sulfite-oxidase-deficiency
#7
Mateus Grings, Alana Pimentel Moura, Belisa Parmeggiani, Julia Tauna Pletsch, Gabriela Miranda Fernandez Cardoso, Pauline Maciel August, Cristiane Matté, Angela T S Wyse, Moacir Wajner, Guilhian Leipnitz
Sulfite accumulates in tissues of patients affected by sulfite oxidase (SO) deficiency, a neurometabolic disease characterized by seizures and progressive encephalopathy, often resulting in early death. We investigated the effects of sulfite on mitochondrial function, antioxidant system, glial reactivity and neuronal damage in rat striatum, as well as the potential protective effects of bezafibrate on sulfite-induced toxicity. Thirty-day-old rats were intrastriatally administered with sulfite (2μmol) or NaCl (2μmol; control) and euthanized 30min after injection for evaluation of biochemical parameters and western blotting, or 7days after injection for analysis of glial reactivity and neuronal damage...
May 18, 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/28529009/assembly-defects-of-multiple-respiratory-chain-complexes-in-a-child-with-cardiac-hypertrophy-associated-with-a-novel-acad9-mutation
#8
Konstantina Fragaki, Annabelle Chaussenot, Audrey Boutron, Sylvie Bannwarth, Cecile Rouzier, Brigitte Chabrol, Veronique Paquis-Flucklinger
Patients carrying Acyl-CoA dehydrogenase 9 (ACAD9) mutations reported to date mainly present with severe hypertrophic cardiomyopathy and isolated complex I (CI) dysfunction. Here we report a novel ACAD9 mutation in a young girl presenting with severe hypertrophic cardiomyopathy, isolated CI deficiency and interestingly multiple respiratory chain complexes assembly defects. We show that ACAD9 analysis has to be performed in first intention in patients presenting with cardiac hypertrophy even in the presence of multiple assembly defects...
May 4, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28528862/crocetin-restores-diabetic-endothelial-progenitor-cell-dysfunction-by-enhancing-no-bioavailability-via-regulation-of-pi3k-akt-enos-and-ros-pathways
#9
Wei Cao, Jinjin Cui, Shufeng Li, Donghui Zhang, Yibo Guo, Qiannan Li, Ying Luan, Xian Liu
AIMS: Endothelial progenitor cell (EPC) dysfunction underlies a critical risk factor in diabetic vascular complications due to function defect in restoring endothelium injury. Crocetin has attracted increasing attention in several vascular-related diseases. In present study, we aimed to explore the role of crocetin in diabetic EPC dysfunction. MAIN METHODS: EPCs were isolated from bone marrow in diabetic mice and identified using the fluorescence staining and flow cytometry...
May 18, 2017: Life Sciences
https://www.readbyqxmd.com/read/28528849/extracellular-low-n-oligomers-of-tau-cause-selective-synaptotoxicity-without-affecting-cell-viability
#10
Senthilvelrajan Kaniyappan, Ram Reddy Chandupatla, Eva-Maria Mandelkow, Eckhard Mandelkow
INTRODUCTION: Tau-mediated toxicity in Alzheimer's disease is thought to operate through low-n oligomers, rather than filamentous aggregates. However, the nature of oligomers and pathways of toxicity are poorly understood. Therefore, we investigated structural and functional aspects of highly purified oligomers of a pro-aggregant tau species. METHODS: Purified oligomers of the tau repeat domain were characterized by biophysical and structural methods. Functional aspects were investigated by cellular assays ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) bromide assay of cell viability, lactate dehydrogenase release assay [for cell toxicity], reactive oxygen species production, and calcium assay), combined with analysis of neuronal dendritic spines exposed to oligomers...
May 18, 2017: Alzheimer's & Dementia: the Journal of the Alzheimer's Association
https://www.readbyqxmd.com/read/28528383/pretreatment-of-hardwood-and-miscanthus-with-trametes-versicolor-for-bioenergy-conversion-and-densification-strategies
#11
Ryan M Kalinoski, Hector D Flores, Sunil Thapa, Erin R Tuegel, Michael A Bilek, Evelin Y Reyes-Mendez, Michael J West, Tim J Dumonceaux, Thomas Canam
The pretreatment of plant biomass negatively impacts the economics of many bioenergy and bioproduct processes due to the thermochemical requirements for deconstruction of lignocelluluose. An effective strategy to reduce these severity requirements is to pretreat the biomass with white-rot fungi, such as Trametes versicolor, which have the innate ability to deconstruct lignocellulose with a suite of specialized enzymes. In the present study, the effects of 12 weeks of pretreatment with a wild-type strain (52J) and a cellobiose dehydrogenase-deficient strain (m4D) of T...
May 20, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28528091/antiproliferative-activity-of-ontario-grown-onions-against-colorectal-adenocarcinoma-cells
#12
Abdulmonem I Murayyan, Cynthya M Manohar, Gordon Hayward, Suresh Neethirajan
Cancer is the leading cause of mortality in Canada and other industrialized nations; the development of new/improved cancer therapies is desperately needed and continues to be a major focus of cancer research. Flavonoids, which are found in high levels in onions, have been shown to exert antiproliferative and potentially anti-cancer activities. To test their therapeutic potential, we assessed the antiproliferative, cytotoxic, apoptosis-inducing, and anti-migratory activities of five onion varieties grown in Ontario against human adenocarcinoma (Caco-2) cells...
June 2017: Food Research International
https://www.readbyqxmd.com/read/28528082/discovery-of-bi-135585-an-in-vivo-efficacious-oxazinanone-based-11%C3%AE-hydroxysteroid-dehydrogenase-type-1-inhibitor
#13
Linghang Zhuang, Colin M Tice, Zhenrong Xu, Wei Zhao, Salvacion Cacatian, Yuan-Jie Ye, Suresh B Singh, Peter Lindblom, Brian M McKeever, Paula M Krosky, Yi Zhao, Deepak Lala, Barbara A Kruk, Shi Meng, Lamont Howard, Judith A Johnson, Yuri Bukhtiyarov, Reshma Panemangalore, Joan Guo, Rong Guo, Frank Himmelsbach, Bradford Hamilton, Annette Schuler-Metz, Heike Schauerte, Richard Gregg, Gerard M McGeehan, Katerina Leftheris, David A Claremon
A potent, in vivo efficacious 11β hydroxysteroid dehydrogenase type 1 (11β HSD1) inhibitor (11j) has been identified. Compound 11j inhibited 11β HSD1 activity in human adipocytes with an IC50 of 4.3nM and in primary human adipose tissue with an IC80 of 53nM. Oral administration of 11j to cynomolgus monkey inhibited 11β HSD1 activity in adipose tissue. Compound 11j exhibited >1000× selectivity over other hydroxysteroid dehydrogenases, displays desirable pharmacodynamic properties and entered human clinical trials in 2011...
April 29, 2017: Bioorganic & Medicinal Chemistry
https://www.readbyqxmd.com/read/28527826/metabolic-pathway-analysis-of-the-xylose-metabolizing-yeast-protoplast-fusant-zlyrhz7
#14
Jingping Ge, Renpeng Du, Gang Song, Yuhuan Zhang, Wenxiang Ping
Xylose is the second major fermentable sugar present in hard woods and herbs (after d-glucose). Therefore, efficient conversion of xylose to ethanol is essential for the commercialization of lignocellulosic ethanol, which may provide an ideal alternative to fossil fuels in the future. ZLYRHZ7 is a fusant produced by protoplast fusion between two different yeast species, Saccharomyces cerevisiae W5 and Candida shehatae 20335, which is able to utilize xylose to produce ethanol. To improve ethanol production and to quantitatively analyze metabolic pathway in ZLYRHZ7, we used high performance liquid chromatography (HPLC) to assess the utilization rates of xylose, xylitol, and xylulose, and to measure ethanol yields using xylose, xylitol, and xylulose as sole carbon sources...
May 17, 2017: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/28527624/understanding-aerobic-anaerobic-metabolism-in-caldibacillus-debilis-through-a-comparison-with-model-organisms
#15
Scott Wushke, Victor Spicer, Xiang Li Zhang, Brian Fristensky, Oleg V Krokhin, David B Levin, Nazim Cicek, Richard Sparling
Caldibacillus debilis GB1 is a facultative anaerobe isolated from a thermophilic aero-tolerant cellulolytic enrichment culture. There is a lack of representative proteomes of facultative anaerobic thermophilic Bacillaceae, exploring aerobic/anaerobic expression. The C. debilis GB1 genome was sequenced and annotated, and the proteome characterized under aerobic and anaerobic conditions while grown on cellobiose. The draft sequence of C. debilis GB1 contains a 3,340,752 bp chromosome and a 5,386 bp plasmid distributed over 49 contigs...
April 13, 2017: Systematic and Applied Microbiology
https://www.readbyqxmd.com/read/28527616/catalase-increases-ethanol-oxidation-through-the-purine-catabolism-in-rat-liver
#16
Daniel Villalobos-García, Rolando Hernández-Muñoz
Hepatic ethanoloxidationincreases according to its concentration and is raised to near-saturation levels of alcohol dehydrogenase (ADH); therefore, re-oxidation of NADH becomes rate limiting in ethanol metabolism by the liver. Adenosine is able to increase liver ethanol oxidation in both in vivo and in vitro conditions; the enhancement being related with the capacity of the nucleoside to accelerate the transport of cytoplasmic reducing equivalents to mitochondria, by modifying the subcellular distribution of the malate-aspartate shuttle components...
May 17, 2017: Biochemical Pharmacology
https://www.readbyqxmd.com/read/28526813/radiomic-model-for-predicting-mutations-in-the-isocitrate-dehydrogenase-gene-in-glioblastomas
#17
Kevin Li-Chun Hsieh, Cheng-Yu Chen, Chung-Ming Lo
The present study proposed a computer-aided diagnosis system based on radiomic features extracted through magnetic resonance imaging to determine the isocitrate dehydrogenase status in glioblastomas. Magnetic resonance imaging data were obtained from 32 patients with wild-typeisocitrate dehydrogenase and 7 patients with mutant isocitrate dehydrogenase in glioblastomas. Radiomic features, namely morphological, intensity, and textural features, were extracted from the tumor area of each patient. The feature sets were evaluated using a logistic regression classifier to develop a prediction model...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28526793/genomic-analysis-of-calderihabitans-maritimus-kkc1-a-thermophilic-hydrogenogenic-carboxydotrophic-bacterium-isolated-from-marine-sediment
#18
Kimiho Omae, Yasuko Yoneda, Yuto Fukuyama, Takashi Yoshida, Yoshihiko Sako
Calderihabitans maritimus KKC1 is a thermophilic hydrogenogenic carboxydotroph isolated from a submerged marine caldera. Here, we describe the de novo sequencing and feature analysis of the C. maritimus KKC1 genome. Genome-based phylogenetic analysis confirmed that C. maritimus KKC1 was most closely related to the genus Moorella, which includes well-studied acetogenic members. Comparative genomic analysis revealed that, like Moorella, C. maritimus KKC1 retained both the CO2-reducing Wood-Ljungdahl pathway and energy-converting hydrogenase-based module activated by reduced ferredoxin, but lacked the HydABC and NfnAB electron bifurcating enzymes and pyruvate:ferredoxin oxidoreductase required for ferredoxin reduction during acetogenic growth...
May 19, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28526614/human-aldehyde-dehydrogenase-3a1-aldh3a1-exhibits-chaperone-like-function
#19
Georgia-Persephoni Voulgaridou, Ilias Tsochantaridis, Theodora Mantso, Rodrigo Franco, Mihalis I Panayiotidis, Aglaia Pappa
Aldehyde dehydrogenase 3A1 (ALDH3A1) is a metabolic enzyme that catalyzes the oxidation of various aldehydes. Certain types of epithelial tissues in mammals, especially those continually exposed to environmental stress (e.g., corneal epithelium), express ALDH3A1 at high levels and its abundance in such tissues is perceived to help to maintain cellular homeostasis under conditions of oxidative stress. Metabolic as well as non-metabolic roles for ALDH3A1 have been associated with its mediated resistance to cellular oxidative stress...
May 16, 2017: International Journal of Biochemistry & Cell Biology
https://www.readbyqxmd.com/read/28526345/facile-fabrication-of-3d-porous-hybrid-sphere-by-co-immobilization-of-multi-enzyme-directly-from-cell-lysates-as-an-efficient-and-recyclable-biocatalyst-for-asymmetric-reduction-with-coenzyme-regeneration-in-situ
#20
MinganYu, Duqiang Liu, Lili Sun, Jing Li, Qian Chen, Lijun Pan, Jingchuan Shang, Shurong Zhang, Wei Li
Ni(2+)-agarose bead-wrapped multi-enzyme/inorganic hybrid sphere composed of the immobilized enzymes as organic component and NaH2PO4 and NaCl as inorganic component was developed by co-immobilizing extracellular His-tagged 3-quinuclidinone reductases and glucose dehydrogenase without pre-purification. The resulting biocatalysts has 3D porous architectures as confirmed by SEM and FESEM, and it enabled the continuous biotransformation of 3-quinuclidone to (R)-3-quinuclidinol with cofactor regeneration in situ...
May 16, 2017: International Journal of Biological Macromolecules
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