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https://www.readbyqxmd.com/read/29692094/-dynamics-of-and-relationship-between-fat-accumulation-and-enzyme-activities-of-precocious-walnut
#1
Shuang Zhao, Yang Liu, Han Li, Yi Lan Chen, Bao Guo Li, Guo Hui Qi, Xue Mei Zhang
Two precocious walnut varieties, Lvling and Lvzao were used as materials in this study, the kernel fat contents and 3 related enzyme activities in different development periods after flowe-ring 50 days were analyzed. The key enzymes affecting walnut fat in different periods were illumina-ted. The results showed that the kernel fat accumulation trends of the two varieties were basically the same. The kernel began to solidify 50 days after flowering, and the increase in kernel fat content was rapid 60-90 days after flowering, slowed down 90-120 days after flowering, and stopped 120-130 days after flowering...
November 2017: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/29692070/-effects-of-elevated-ozone-concentrations-on-enzyme-activities-and-organic-acids-content-in-wheat-rhizospheric-soil
#2
Wei Qin Yin, Hao Qi Jing, Ya Bo Wang, Si Yu Wei, Yue Sun, Sheng Sen Wang, Xuai Zhi Wang
The elevated concentration of tropospheric ozone (O3 ) is an important global climate change driver, with adverse impacts on soil ecological environment and crop growth. In this study, a pot experiment was carried out in an open top chamber (OTC), to investigate the effects of elevated ozone concentration on soil enzyme activities (catalase, polyphenol oxidase, dehydrogenase and invertase), organic acids contents (oxalic acid, citric acid and malic acid) at different growth stages (tillering, jointing, heading and ripening stages) of wheat, and combined with the rhizospheric soil physicochemical properties and plant root characteristics to analyze the underlying reasons...
February 2018: Ying Yong Sheng Tai Xue Bao, the Journal of Applied Ecology
https://www.readbyqxmd.com/read/29691939/genome-sequencing-reveals-a-novel-genetic-mechanism-underlying-dihydropyrimidine-dehydrogenase-deficiency-a-novel-missense-variant-c-1700g-a-and-a-large-intragenic-inversion-in-dpyd-spanning-intron-8-to-intron-12
#3
André B P van Kuilenburg, Maja Tarailo-Graovac, Judith Meijer, Britt Drogemoller, Jerry Vockley, Dirk Maurer, Doreen Dobritzsch, Colin J Ross, Wyeth Wasserman, Rutger Meinsma, Lida Zoetekouw, Clara D M van Karnebeek
Dihydropyrimidine dehydrogenase (DPD) deficiency is associated with a variable clinical presentation. A family with three DPD deficient patients presented with unusual clinical phenotypes including pregnancy-induced symptoms, transient visual impairment, severe developmental delay, cortical blindness and delayed myelination in the brain. DPYD Sanger sequencing showed heterozygosity for the c.1905+1G > A mutation and a novel missense variant c.1700G > A (p.G456E). The recombinantly-expressed p.G456E DPD variant showed increased temperature lability probably caused by structural rearrangements within the DPD protein...
April 24, 2018: Human Mutation
https://www.readbyqxmd.com/read/29691935/mitochondria-and-wolbachia-titers-are-positively-correlated-during-maternal-transmission
#4
Lucas P Henry, Irene L G Newton
Mothers provide their offspring with symbionts. Maternally transmitted, intracellular symbionts must disperse from mother to offspring with other cytoplasmic elements, like mitochondria. Here, we investigated how the intracellular symbiont Wolbachia interacts with mitochondria during maternal transmission. Mitochondria and Wolbachia may interact antagonistically and compete as each population tries to ensure its own evolutionary success. Alternatively, mitochondria and Wolbachia may cooperate as both benefit from ensuring the fitness of the mother...
April 25, 2018: Molecular Ecology
https://www.readbyqxmd.com/read/29691575/-protective-effects-of-endogenous-carbon-monoxide-against-myocardial-ischemia-reperfusion-injury-in-rats
#5
Zhen Zhou, Shuang Ma, Jie Liu, Qiao-Rong Ji, Cheng-Zhu Cao, Xiao-Na Li, Feng Tang, Wei Zhang
The present study is aimed to explore the effects of endogenous carbon monoxide on the ischemia-reperfusion in rats. Wistar rats were intraperitoneally injected with protoporphyrin cobalt chloride (CoPP, an endogenous carbon monoxide agonist, 5 mg/kg), zinc protoporphyrin (ZnPP, an endogenous carbon monoxide inhibitor, 5 mg/kg) or saline. Twenty-four hours after injection, the myocardial ischemia-reperfusion model was made by Langendorff isolated cardiac perfusion system, and cardiac function parameters were collected...
April 25, 2018: Sheng Li Xue Bao: [Acta Physiologica Sinica]
https://www.readbyqxmd.com/read/29691295/metformin-targets-mitochondrial-glycerophosphate-dehydrogenase-mgpdh-to-control-rate-of-oxidative-phosphorylation-and-growth-of-thyroid-cancer-in-vitro-and-in-vivo
#6
Joanna Klubo-Gwiezdzinska, Shilpa Thakur, Brianna Daley, Kelli Gaskins, Vasyl V Vasko, Myriem Boufraqech, Dhaval Patel, Carole Sourbier, Jeff M Reece, Sheue-Yann Cheng, Electron Kebebew, Sunita K Agarwal
PURPOSE: Mitochondrial glycerophosphate dehydrogenase (mGPDH) is the key enzyme connecting oxidative phosphorylation (OXPHOS) and glycolysis as well as a target of the antidiabetic drug metformin (MF) in the liver. There are no data on the expression and role of mGPDH as a metformin target in cancer. In this study, we evaluated mGPDH as a potential target of metformin in thyroid cancer and investigated its contribution in thyroid cancer metabolism. EXPERIMENTAL DESIGN: We analyzed mGPDH expression in 253 thyroid cancer and normal tissues by immunostaining and examined its expression and localization in thyroid cancer-derived cell lines (FTC133, BCPAP) by confocal microscopy...
April 24, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29691195/d-lactate-electrochemical-biosensor-prepared-by-immobilization-of-thermostable-dye-linked-d-lactate-dehydrogenase-from-candidatus-caldiarchaeum-subterraneum
#7
Takenori Satomura, Junji Hayashi, Hiroaki Sakamoto, Takuro Nunoura, Yoshihiro Takaki, Ken Takai, Hideto Takami, Toshihisa Ohshima, Haruhiko Sakuraba, Shin-Ichiro Suye
A stable d-lactate electrochemical sensing system was developed using a dye-linked d-lactate dehydrogenase (Dye-DLDH) from an uncultivated thermophilic archaeon, Candidatus Caldiarchaeum subterraneum. To develop the system, the putative gene encoding the Dye-DLDH from Ca. Caldiarchaeum subterraneum was overexpressed in Escherichia coli, and the expressed product was purified. The recombinant enzyme was a highly thermostable Dye-DLDH that retained full activity after incubation for 10 min at 70°C. The electrode for detection of d-lactate was prepared by immobilizing the thermostable Dye-DLDH and multi-walled carbon nanotube (MWCNT) within Nafion membrane...
April 21, 2018: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/29690663/-expression-of-succinate-dehydrogenase-subunit-protein-in-succinate-dehydrogenase-deficient-gastrointestinal-stromal-tumors
#8
Y J Zhang, Y H Cheng, L X Guo, N Q Wang, H Liu
Objective: To investigate the expression of succinate dehydrogenase complex subunit protein in succinate dehydrogenase-deficient gastrointestinal stromal tumors (SDH-deficient GISTs). Methods: Three hundred fifty-two cases of GISTs were collected from January 2003 to January 2017 at the Affiliated Hospital of Guizhou Medical University and West China Hospital of Sichuan University.The expression of succinate dehydrogenase subunit protein was detected by immunohistochemical EnVision technique in 352 cases of GISTs, and the negative cases were analyzed for clinicopathologic features and outcome...
April 8, 2018: Zhonghua Bing Li Xue za Zhi Chinese Journal of Pathology
https://www.readbyqxmd.com/read/29690651/betulin-3-28-diphosphate-as-a-component-of-combination-cytostatic-drugs-for-the-treatment-of-ehrlich-ascites-carcinoma-in-vitro-and-in-vivo-experiments
#9
Olga Vorobyova, Olga Deryabina, Darina Malygina, Nadezhda Plotnikova, Anna Solovyeva, Kseniya Belyaeva, Nina Melnikova
The activity of betulin-3,28-diphosphate (BDP) in combination with the cytostatics such as 5-fluorouracil (5-FU) and hydrazine sulfate (HS) was demonstrated by using the transplanted Ehrlich ascites carcinoma (EAC) in mice. The dose-dependent effect of combination drugs BDP + HS and BDP + 5-FU was revealed by in vitro experiments on rats. The synergetic effect of HS and BDP on oxidative stress and energy metabolism was established. The malonic dialdehyde (MDA) level both in plasma and erythrocytes decreased by 87 ± 2%, and the superoxide dismutase (SOD) activity increased by 105 ± 7% in comparison with the control...
April 23, 2018: Scientia Pharmaceutica
https://www.readbyqxmd.com/read/29689791/a-new-species-of-fan-throated-lizard-of-the-genus-sitana-cuvier-1829-from-coastal-kerala-southern-india
#10
Kalesh Sadasivan, M B Ramesh, Muhamed Jafer Palot, Mayuresh Ambekar, Zeeshan A Mirza
We here describe Sitana attenboroughii sp. nov., a new species of fan-throated lizard of the genus Sitana Cuvier, 1829 from coastal Kerala in southern India. The new species morphologically is closer to Sitana visiri Deepak, 2016 (in Deepak et al. 2016a), however, differs in having higher numbers of ventral scales and a comparatively short but richly colored dewlap. Genetically the new species shows affinity to Sitana marudhamneydhal Deepak, Khandekar, Varma Chaitanya, 2016 from which it differs in an uncorrected pairwise sequence divergence of 2...
January 21, 2018: Zootaxa
https://www.readbyqxmd.com/read/29689567/ampk-contributes-to-cardioprotective-effects-of-pterostilbene-against-myocardial-ischemia-reperfusion-injury-in-diabetic-rats-by-suppressing-cardiac-oxidative-stress-and-apoptosis
#11
Ramoji Kosuru, Yin Cai, Vidya Kandula, Dan Yan, Chunyan Wang, Hong Zheng, Yalan Li, Michael G Irwin, Sanjay Singh, Zhengyuan Xia
BACKGROUND/AIMS: Pterostilbene (PT) exerts antidiabetic effects by decreasing blood glucose and modulating lipid metabolism and has been shown to attenuate myocardial ischemia-reperfusion (IR) injury in non-diabetic subjects. However, whether PT can protect against myocardial IR injury in diabetes is unknown. AMPK stimulation is indispensable in offering cardioprotection against myocardial IR injury in diabetes by limiting cardiac apoptosis. Thus, we hypothesized that PT may confer protection against myocardial IR injury in diabetes via AMPK activation...
April 18, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29689564/pulmonary-exposure-to-particulate-matter-pm2-5-affects-the-sensitivity-to-myocardial-ischemia-reperfusion-injury-through-farnesoid-x-receptor-induced-autophagy
#12
Fei Tong, Hua Zhang
BACKGROUND/AIMS: The purpose of this study was to investigate the effect of administering particulate matter (PM2.5) to the lungs on the sensitivity to myocardial ischemia/reperfusion injury (MI/RI) and the role of farnesoid-X-receptor (FXR)-induced autophagy in this process. METHODS: Male Sprague Dawley (SD) rats were subjected to 45 min of ischemia, and the lungs were exposed to 2.0 mg of PM2.5 in 0.3 mL of normal saline 5 min before reperfusion. After 24 h of reperfusion, the blood and myocardium were collected, and the myocardial infarct sizes, activities of serum creatine kinase (CK) and lactate dehydrogenase (LDH), and levels of serum high sensitivity C-reactive protein (hsCRP), interleukin-4 (IL-4), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), cardiac troponin T (cTnT), P-selectin and D-dimer were measured via biochemical analysis...
April 19, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/29689531/genomic-and-transcriptomic-alterations-in-leishmania-donovani-lines-experimentally-resistant-to-antileishmanial-drugs
#13
Alberto Rastrojo, Raquel García-Hernández, Paola Vargas, Esther Camacho, Laura Corvo, Hideo Imamura, Jean-Claude Dujardin, Santiago Castanys, Begoña Aguado, Francisco Gamarro, Jose M Requena
Leishmaniasis is a serious medical issue in many countries around the World, but it remains largely neglected in terms of research investment for developing new control and treatment measures. No vaccines exist for human use, and the chemotherapeutic agents currently used are scanty. Furthermore, for some drugs, resistance and treatment failure are increasing to alarming levels. The aim of this work was to identify genomic and trancriptomic alterations associated with experimental resistance against the common drugs used against VL: trivalent antimony (SbIII , S line), amphotericin B (AmB, A line), miltefosine (MIL, M line) and paromomycin (PMM, P line)...
April 13, 2018: International Journal for Parasitology, Drugs and Drug Resistance
https://www.readbyqxmd.com/read/29689346/b-type-natriuretic-peptide-attenuates-endoplasmic-reticulum-stress-in-h9c2-cardiomyocytes-underwent-hypoxia-reoxygenation-injury-under-high-glucose-high-fat-conditions
#14
Pan Chang, Mingyang Zhang, Xiaomeng Zhang, Guohua Li, Haiyan Hu, Juan Wu, Xihui Wang, Zihua Yang, Jing Zhang, Weiguo Chen, Minggang Ren, Xin Li, Miaozhang Zhu, Baoying Chen, Jun Yu
Exogenously administered B-type natriuretic peptide (BNP) has been shown to provide cardioprotection against various heart diseases. However, the underlying mechanisms remain elusive. This study explores whether BNP exerts its cardioprotection against hypoxia/reoxygenation (H/R) injury under high glucose/high fat (HG/HF) conditions in cardiac H9c2 cells and uncovers the underlying mechanisms. Our data revealed that BNP significantly increased the cell viability and decreased the release of lactate dehydrogenase (LDH) and creatine kinase (CK), with a maximal effect at the BNP concentration of 10-7  mol/L...
April 21, 2018: Peptides
https://www.readbyqxmd.com/read/29689131/unsupervised-statistically-based-systems-biology-approach-for-unraveling-the-genetics-of-complex-traits-a-demonstration-with-ethanol-metabolism
#15
Ryan Lusk, Laura M Saba, Lauren A Vanderlinden, Vaclav Zidek, Jan Silhavy, Michal Pravenec, Paula L Hoffman, Boris Tabakoff
BACKGROUND: A statistical pipeline was developed and used for determining candidate genes and candidate gene co-expression networks involved in two alcohol (i.e., ethanol) metabolism phenotypes, namely alcohol clearance and acetate area under the curve (AUC) in a recombinant inbred (HXB/BXH) rat panel. The approach was also used to provide an indication of how ethanol metabolism can impact the normal function of the identified networks. METHODS: RNA was extracted from alcohol-naïve liver tissue of 30 strains of HXB/BXH recombinant inbred rats...
April 24, 2018: Alcoholism, Clinical and Experimental Research
https://www.readbyqxmd.com/read/29688811/trehalose-6-phosphate-phosphatases-of-pseudomonas-aeruginosa
#16
Megan Cross, Sonja Biberacher, Suk-Youl Park, Siji Rajan, Pasi Korhonen, Robin B Gasser, Jeong-Sun Kim, Mark J Coster, Andreas Hofmann
The opportunistic bacterium Pseudomonas aeruginosa has been recognized as an important pathogen of clinical relevance and is a leading cause of hospital-acquired infections. The presence of a glycolytic enzyme in Pseudomonas, which is known to be inhibited by trehalose 6-phosphate (T6P) in other organisms, suggests that these bacteria may be vulnerable to the detrimental effects of intracellular T6P accumulation. In the present study, we explored the structural and functional properties of trehalose 6-phosphate phosphatase (TPP) in P...
April 24, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29688258/isoprenoids-increase-bovine-endometrial-stromal-cell-tolerance-to-the-cholesterol-dependent-cytolysin-from-trueperella-pyogenes
#17
Sholeem Griffin, Gareth D Healey, I Martin Sheldon
Preventing postpartum uterine disease depends on the ability of endometrial cells to tolerate the presence of the bacteria that invade the uterus after parturition. Postpartum uterine disease and endometrial pathology in cattle is most associated with the pathogen Trueperella pyogenes. Trueperella pyogenes secretes a cholesterol-dependent cytolysin, pyolysin, which causes cytolysis by forming pores in the plasma membrane of endometrial stromal cells. The aim of the present study was to identify cell-intrinsic pathways that increase bovine endometrial stromal cell tolerance to pyolysin...
April 24, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29688087/modification-of-translationally-controlled-tumor-protein-derived-protein-transduction-domain-for-improved-intranasal-delivery-of-insulin
#18
Hae-Duck Bae, Joohyun Lee, Kyu-Yeon Jun, Youngjoo Kwon, Kyunglim Lee
Carrier peptides, termed protein transduction domains (PTDs), serve as provide promising vehicles for intranasal delivery of macromolecular drugs. A mutant PTD derived from human translationally controlled tumor protein (TCTP-PTD 13, MIIFRALISHKK) was reported to provide enhanced intranasal delivery of insulin. In this study, we tested whether its efficiency could be further improved by replacing amino acids in TCTP-PTD 13 or changing the amino acids in the carrier peptides from the l- to the d-form. We assessed the pharmacokinetics of PTD-mediated transmucosal delivery of insulin in normal rats and the activity of insulin in alloxan-induced diabetic rats...
November 2018: Drug Delivery
https://www.readbyqxmd.com/read/29688059/comparative-study-on-effects-of-four-energy-plants-growth-on-chemical-fractions-of-heavy-metals-and-activity-of-soil-enzymes-in-copper-mine-tailings
#19
Jie Zhang, Shiyong Yang, Hongfei Yang, Yongjie Huang, Liming Zheng, Jing Yuan, Shoubiao Zhou
Four gramineous energy plants, Miscanthus sacchariflorus, M. floridulus, Phragmites australis, and Arundo donax were grown on copper tailings in the field for four years. Their phytoremediation potential was examined in terms of their effects on the fractions of heavy metals and soil enzyme activities. Results showed that plantation of these four gramineous plants has improved the proportion of organic material (OM)-binding fraction of heavy metals in copper tailings as a whole, and reduced the proportion of exchangeable and residual fractions...
May 12, 2018: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29687413/protective-effect-of-apigenin-on-acrylonitrile-induced-inflammation-and-apoptosis-in-testicular-cells-via-the-nf-%C3%AE%C2%BAb-pathway-in-rats
#20
Yuhui Dang, Zhilan Li, Qian Wei, Ruiping Zhang, Hongli Xue, Yingmei Zhang
Apigenin (AP) as a plant flavonoid is found to attenuate acrylonitrile (ACN) toxicity by reducing ROS production and inhibiting apoptosis. Therefore, the present study aimed to evaluate the role of AP on ACN-induced inflammation and apoptosis in germ cells and whether it is through the NF-κB signaling pathway. AP increased the concentrations of lactate dehydrogenase isozyme (LDH) and sorbitol dehydrogenase (SDH), while the concentrations of interleukin β (IL-1β), tumor-necrosis factor-α (TNF-α), and interleukin-6 (IL-6) were significantly reduced...
April 23, 2018: Inflammation
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