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Natasha L Maia, Mariana de Barros, Leandro L de Oliveira, Silvia A Cardoso, Marcelo H Dos Santos, Fabio A Pieri, Teodorico C Ramalho, Elaine F F da Cunha, Maria A S Moreira
Mastitis is an inflammation of the mammary gland that causes major losses in the dairy industry. Streptococcus spp. are among the main agents of this disease. Increased resistance to antibiotics is one of the causes of therapeutic failure. Plants, due to their broad chemodiversity, are an interesting source of new molecules with antibacterial activity. Using these compounds along with traditional antibiotics is a possible method for reversing resistance. The objective of this work was to determine the interactions between the activities of guttiferone-A and 7-epiclusianone, two active substances isolated from the fruits of Garcinia brasiliensis , and traditional antibiotics against Streptococcus spp...
2018: Frontiers in Microbiology
Alfredo Ponce-de-Leon, Eduardo Rodríguez-Noriega, Rayo Morfín-Otero, Dora P Cornejo-Juárez, Juan C Tinoco, Areli Martínez-Gamboa, Carmen J Gaona-Tapia, M Lourdes Guerrero-Almeida, Alexandra Martin-Onraët, José Luis Vallejo Cervantes, José Sifuentes-Osornio
Antimicrobial resistance is an increasing worldwide concern, which poses unique challenges for the effective prevention and treatment of several infections, especially the ones triggered by organisms producing extended-spectrum β-lactamases (ESBL). Here, we present the surveillance results of the Study for Monitoring Antimicrobial Resistance Trends (SMART) of Gram-negative bacilli isolated from intra-abdominal infections (IAI, n = 1,235) and urinary-tract infections (UTI, n = 2,682), collected in Mexico from 2009 to 2015...
2018: PloS One
Hazem H Ramadan, Charlene R Jackson, Samaa A Taha, Amira A Moawad, John B Barrett, Tiffanie A Woodley
BACKGROUND: Chickens are considered potential reservoirs for human extraintestinal infections with pathogenic Escherichia coli. However, information about genetic relatedness between E. coli from healthy chickens and human patients is still limited. METHODS AND RESULTS: In this study, clinical samples from patients with extraintestinal infections and healthy broiler chickens were collected from geographically related locations in Egypt during the 2nd half of 2015...
June 21, 2018: Vector Borne and Zoonotic Diseases
Olfa Dziri, Raoudha Dziri, Abderrahmen Maraoub, Chedly Chouchani
Extended-spectrum beta-lactamase producing Enterobacteriaceae present a real problem worldwide. We aimed to investigate the molecular mechanisms of resistance to antibiotics among Klebsiella pneumoniae clinical isolates collected from a Hospital in the southeast of Tunisia. Eighteen cephalosporin-resistant K. pneumoniae were recovered between April 2015 and August 2016. Molecular characterization of antimicrobial resistance encoding genes was performed by PCR and sequencing. Results revealed several types of Ambler class A β-lactamase encoding genes among our isolates: [blaCTXM-15 (15), blaSHV-28 (6), blaSHV-1 (2), blaSHV-148 (1), blaSHV-61 (1), blaSHV-76 (1), blaSHV-186 (1), blaTEM-1 (8)]...
June 21, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Kwang Won Seo, Young Ju Lee
Antimicrobial resistance has become a serious public health threat throughout the world, and therapeutic options for several infectious diseases are currently limited by the presence of multidrug-resistant (MDR) bacteria. This study was designed to examine the drug resistance patterns, the prevalence of the β-lactamases, and class 1 integrons in MDR Escherichia coli isolates from chicken meat in Korea. Among 200 chicken meat samples, 101 isolates were observed to be positive for E. coli, of which 57 were identified as MDR E...
June 21, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
Branka Bedenić, Mia Slade, Lidija Žele Starčević, Sanda Sardelić, Mirna Vranić-Ladavac, Ana Benčić, Vlasta Zujić Atalić, Maja Bogdan, Marina Bubonja-Šonje, Maja Tomić-Paradžik, Tatjana Tot, Amarela Lukić-Grlić, Domagoj Drenjančević, Dijana Varda-Brkić, Daniela Bandić-Pavlović, Slobodan Mihaljević, Gernot Zarfel, Marija Gužvinec, Rick Conzemius, Ivan Barišić, Arjana Tambić-Andraševic
PURPOSE: A dramatic increase in OXA-48 β-lactamase was observed recently not only in large hospital centres, but also in smaller suburban hospital centres in geographic areas bordering Croatia. The aim of the study was to analyse the epidemiology, the mechanisms of antibiotic resistance and the routes of spread of OXA-48 carbapenemase in Croatia. METHODS: Carbapenemase and other β-lactamase and fluoroquinolone resistance genes were detected by PCR and sequencing...
June 21, 2018: Journal of Medical Microbiology
Daneeya Ekapopphan, Athicha Srisutthakarn, Walasinee Moonarmart, Ruangrat Buddhirongawatr, Norasuthi Bangphoomi
This study aims to determine the microbiological profile and risk factors associated with antimicrobial-resistant bacteria in canine severe corneal ulcers. Thirty-two corneal and conjunctival swabs were collected from dogs with diagnosed severe corneal ulcers that presented to Prasu-Arthorn veterinary teaching hospital in Nakhon Pathom, Thailand from June 2015 to June 2016. Microorganisms were identified by means of genotypic and phenotypic approaches. Of 32 ulcers sampled, 26 (81.3%) yielded culturable microorganisms with 24 bacterial isolates and 7 fungal isolates...
June 20, 2018: Journal of Veterinary Medical Science
Sheyber J Lifonzo-Mucha, Paula E Tamariz-Zamudio, Roky G Champi-Merino
Urinary infections are caused mainly by Escherichia coli (E. coli); indiscriminate use of antibiotics has caused an increase in infections due to extended-spectrum beta-lactamase (ESBL)-producing strains. Aiming to determine the sensitivity to fosfomycin, a study was conducted in ESBL-producing E. coli strains isolated from urine cultures at a hospital in Peru. Two hundred and sixty-six (266) strains of E. coli were collected, which were determined by conventional methods to be ESBL- producing. Sensitivity to fosfomycin was determined through minimum inhibitory concentration with the agar dilution method and the diffusion disc method...
January 2018: Revista Peruana de Medicina Experimental y Salud Pública
Angelo Falconí Sarmiento, Monthy Nolasco Mejia, Alonzo Bedoya Rozas, Catherine Amaro Giraldo, Germán Málaga
A cross-sectional study was conducted aimed at determining the frequency and the risk factors for bacteremia caused by extended-spectrum Beta-Lactamase (ESBL)-producing Enterobacteriaceae in patients hospitalized in a public hospital in Lima. The study included patients over 14 years of age, with positive blood cultures during their hospitalization in Hospital Nacional Cayetano Heredia in 2016. Patients were classified according to the isolated bacterium (ESBL-producing or not). Bacteremia was caused by ESBL-producing Enterobacteriacea in 50...
January 2018: Revista Peruana de Medicina Experimental y Salud Pública
Frédéric Robin, Michel Auzou, Richard Bonnet, Romain Lebreuilly, Christophe Isnard, Vincent Cattoir, François Guérin
The study evaluated the in vitro activity of ceftolozane-tazobactam (C/T) against 94 unique clinical isolates of Enterobacter cloacae complex (ECC). No difference was observed according to the ECC cluster. The in vitro activity greatly varied depending on the β-lactamase-producing profile: 100%, 67% and 19% of wild-type, ESBL-producing, and AmpC-overproducing strains were susceptible to C/T, respectively. The use of C/T could be of interest for the treatment of some infections caused by ESBL-producing AmpC-non-overexpressing ECC isolates...
June 18, 2018: Antimicrobial Agents and Chemotherapy
Elizabeth G Rhee, Matthew L Rizk, Nicole Calder, Marcela Nefliu, Steven J Warrington, Michael S Schwartz, Eric Mangin, Keith Boundy, Pratik Bhagunde, Francheska Colon-Gonzalez, Patricia Jumes, Yang Liu, Joan R Butterton
Relebactam is a novel class A and C β-lactamase inhibitor that is being developed in combination with imipenem/cilastatin for the treatment of serious gram-negative bacterial infections. Here we report on 2 phase 1 randomized, double-blind, placebo-controlled, pharmacokinetics, safety, and tolerability studies of relebactam administered with or without imipenem/cilastatin in healthy participants: 1) a single dose (25 to 1150 mg) and multiple dose (50 to 625 mg every 6 hours [q6h] for 7 to 14 days) escalation study in men and 2) a single dose (125 mg) study in women and elderly...
June 18, 2018: Antimicrobial Agents and Chemotherapy
Safa S Almarzoky Abuhussain, Joseph L Kuti, David P Nicolau
The role of inhalational combination therapy when treating carbapenem resistant Pseudomonas aeruginosa and Klebsiella pneumoniae with newer beta-lactam/beta-lactamase inhibitors has not been established. Using a 72 hour in vitro pharmacodynamic chemostat model, we simulated the human exposures achieved in epithelial lining fluid (ELF) following intravenous ceftazidime-avibactam (CZA) 2.5g q8h alone and in combination with inhaled amikacin (AMK) 400mg q12h, a reformulated aminoglycoside designed for inhalational administration, against 3 P...
June 18, 2018: Antimicrobial Agents and Chemotherapy
Sean M Stainton, Kamilia Abdelraouf, Luke Utley, Michael J Pucci, Troy Lister, David P Nicolau
SPR741 is a novel agent with structural similarity to polymyxins, capable of potentiating the activity of various classes of antibiotics. Previously published studies indicated that although Enterobacteriacae isolates had minimal susceptibilities to azithromycin (AZM), the in vitro antimicrobial activity of AZM against Enterobacteriacae was enhanced when combined with SPR741. The current study evaluated the in vivo activity of human-simulated regimens (HSR) of AZM equivalent to clinical doses of 500 mg IV q24h and SPR741 400 mg q8h IV (1h infusion) alone and in combination against multi-drug resistant (MDR) Enterobacteriacae...
June 18, 2018: Antimicrobial Agents and Chemotherapy
Vishal Diwan, Nada Hanna, Manju Purohit, Salesh Chandran, Emilia Riggi, Vivek Parashar, Ashok J Tamhankar, Cecilia Stålsby Lundborg
OBJECTIVES: To characterize the seasonal variation, over one year, in water-quality, antibiotic residue levels, antibiotic resistance genes and antibiotic resistance in Escherichia coli isolates from water and sediment of the Kshipra River in Central India. METHODS: Water and sediment samples were collected from seven selected points from the Kshipra River in the Indian city of Ujjain in the summer, rainy season, autumn and winter seasons in 2014. Water quality parameters (physical, chemical and microbiological) were analyzed using standard methods...
June 17, 2018: International Journal of Environmental Research and Public Health
Jess Vergis, Richa Pathak, Manesh Kumar, R Sunitha, S V S Malik, S B Barbuddhe, Deepak B Rawool
We explored and evaluated for the first time colorimetric nitrocefin assay in conjunction with the double disc test and PCR assay. We suggested the use of nitrocefin assay for rapid screening of ESBL-production by Enteroaggregative Escherichia coli.
June 15, 2018: Journal of Microbiological Methods
Bat-Sheva Gottesman, Pnina Shitrit, Michal Katzir, Michal Chowers
BACKGROUND: Increasing antibiotic resistance in the community results in greater use of empiric broad spectrum antibiotics for patients at hospital admission. As a measure of antibiotic stewardship it is important to identify a patient population that can receive narrow spectrum antibiotics. OBJECTIVES: To evaluate resistance patterns of Escherichia coli bloodstream infection (BSI) from strictly community-acquired infection and the impact of recent antibiotic use on this resistance...
June 2018: Israel Medical Association Journal: IMAJ
Carla Dias, Anabela Borges, Diana Oliveira, Antonio Martinez-Murcia, Maria José Saavedra, Manuel Simões
Background: The "One Health" concept recognizes that human health and animal health are interdependent and bound to the health of the ecosystem in which they (co)exist. This interconnection favors the transmission of bacteria and other infectious agents as well as the flow of genetic elements containing antibiotic resistance genes. This problem is worsened when pathogenic bacteria have the ability to establish as biofilms. Therefore, it is important to understand the characteristics and behaviour of microorganisms in both planktonic and biofilms states from the most diverse environmental niches to mitigate the emergence and dissemination of resistance...
2018: PeerJ
Danilo Garcia Sanchez, Fernanda Maciel de Melo, Eduardo Angelino Savazzi, Eliana Guedes Stehling
Bacterial resistance occurs by spontaneous mutations or horizontal gene transfer mediated by mobile genetic elements, which represents a great concern. Resistance to β-lactam antibiotics is mainly due to the production of β-lactamases, and an important mechanism of fluoroquinolone resistance is the acquisition plasmid determinants. The aim of this study was to verify the presence of β-lactamase-encoding genes and plasmid-mediated quinolone resistance genes in different water samples obtained from São Paulo state, Brazil...
June 16, 2018: Environmental Monitoring and Assessment
Zishuo Cheng, Pei W Thomas, Lincheng Ju, Alexander Bergstrom, Kelly Mason, Delaney Clayton, Callie Miller, Christopher R Bethel, Jamie VanPelt, David L Tierney, Richard C Page, Robert A Bonomo, Walter Fast, Michael W Crowder
Infections by carbapenem-resistant Enterobacteriaceae (CRE) are difficult to manage owing to broad antibiotic resistance profiles and because of the inability of clinically-used β-lactamase inhibitors to counter the activity of metallo-β-lactamases often harbored by these pathogens. Of particular importance is New Delhi metallo-β-lactamase (NDM), which requires a dinuclear zinc ion cluster for catalytic activity. Here, we compare the structures and functions of clinical NDM variants 1-17. The impact of NDM variants on structure is probed by comparing comparing melting temperature and refolding efficiency and also by spectroscopy (UV-Vis, 1 H-NMR, and EPR) of di-cobalt metalloforms...
June 16, 2018: Journal of Biological Chemistry
Olfa Dziri, Carla Andrea Alonso, Raoudha Dziri, Haythem Gharsa, Abderrahmen Maraoub, Carmen Torres, Chedly Chouchani
Carbapenem resistance in Gram-negative bacteria constitutes a major clinical problem. We propose to characterize molecular features among carbapenem-resistant Gram-negative clinical isolates collected from Southeastern Tunisian Island Hospital. Eighteen carbapenem-resistant clinical isolates (13 Klebsiella pneumoniae, 1 Proteus mirabilis, 1 Enterobacter cloacae, 3 Acinetobacter baumannii) were recovered during April 2015-August 2016. Molecular characterization of antimicrobial resistance was performed by PCR and sequencing...
June 14, 2018: International Journal of Antimicrobial Agents
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