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https://www.readbyqxmd.com/read/29679312/efficient-production-of-secretory-streptomyces-clavuligerus-%C3%AE-lactamase-inhibitory-protein-blip-in-pichia-pastoris
#1
Kin-Ho Law, Man-Wah Tsang, Yuk-Ki Wong, Ming-San Tsang, Pui-Yee Lau, Kwok-Yin Wong, Kwok-Ping Ho, Yun-Chung Leung
β-Lactamase inhibitory protein (BLIP), a low molecular weight protein from Streptomyces clavuligerus, has a wide range of potential applications in the fields of biotechnology and pharmaceutical industry because of its tight interaction with and potent inhibition on clinically important class A β-lactamases. To meet the demands for considerable amount of highly pure BLIP, this study aimed at developing an efficient expression system in eukaryotic Pichia pastoris (a methylotrophic yeast) for production of BLIP...
April 20, 2018: AMB Express
https://www.readbyqxmd.com/read/29675924/new-sts-in-multidrug-resistant-acinetobacter-baumannii-harboring-%C3%AE-lactamases-encoding-genes-isolated-from-brazilian-soils
#2
João Pedro Rueda Furlan, André Pitondo-Silva, Eliana Guedes Stehling
AIMS: We investigated the resistance profile, presence of β-lactamases encoding genes and the clonal relationships in Acinetobacter baumannii isolated from Brazilian soils. METHODS AND RESULTS: Soil isolates of Acinetobacter baumannii were subjected to antimicrobial susceptibility testing by disc diffusion and minimum inhibitory concentration methods. Different β-lactamases encoding genes were screened by PCR and the molecular typing of these isolates was performed through the multilocus sequence typing...
April 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29675838/t4-like-escherichia-coli-phages-from-the-environment-carry-bla-ctx-m
#3
Juliet Roshini Mohan Raj, Rajeshwari Vittal, Prashant Huilgol, Udaya Bhat, Indrani Karunasagar
The resistance determinant blaCTX -M has many variants and has been the most commonly reported gene in clinical isolates of extended spectrum beta-lactamase (ESBL) producing Escherichia coli. Phages have been speculated as potential reservoirs of resistance genes and efficient vehicles for horizontal gene transfer. The objective of the study was to determine the prevalence and characterize bacteriophages that harbour the resistance determinant blaCTX -M . E.coli specific bacteriophages were isolated from 15 samples including soil and water across Mangaluru, India using bacterial hosts that were sensitive to β-lactams...
April 20, 2018: Letters in Applied Microbiology
https://www.readbyqxmd.com/read/29675787/meropenem-vaborbactam-a-carbapenem-and-beta-lactamase-inhibitor-with-activity-against-carbapenem-resistant-enterobacteriaceae
#4
REVIEW
Young R Lee, Nathaniel T Baker
Meropenem-vaborbactam is a carbapenem and β-lactamase inhibitor combination that is newly indicated for the treatment of complicated urinary tract infections (cUTI), including adult pyelonephritis. Vaborbactam was developed due to emergence of carbapenem-resistant strains of Enterobacteriaceae. In a phase I trial, patients that received meropenem-vaborbactam 2-2 g intravenously over 3 h every 8 h, Cmax was 58.2 ± 10.8 μg/mL for meropenem and 59.0 ± 8.4 μg/mL for vaborbactam. AUC0-8 was 186 ± 33...
April 19, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29675014/the-resistome-of-low-impacted-marine-environments-is-composed-by-distant-metallo-%C3%AE-lactamases-homologs
#5
Erica L Fonseca, Bruno G N Andrade, Ana C P Vicente
The worldwide dispersion and sudden emergence of new antibiotic resistance genes (ARGs) determined the need in uncovering which environment participate most as their source and reservoir. ARGs closely related to those currently found in human pathogens occur in the resistome of anthropogenic impacted environments. However, the role of pristine environment as the origin and source of ARGs remains underexplored and controversy, particularly, the marine environments represented by the oceans. Here, due to the ocean nature, we hypothesized that the resistome of this pristine/low-impacted marine environment is represented by distant ARG homologs...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29675000/exploring-additional-dimensions-of-complexity-in-inhibitor-design-for-serine-%C3%AE-lactamases-mechanistic-and-intra-and-inter-molecular-chemistry-approaches
#6
REVIEW
Focco van den Akker, Robert A Bonomo
As a bacterial resistance strategy, serine β-lactamases have evolved from cell wall synthesizing enzymes known as penicillin-binding proteins (PBP), by not only covalently binding β-lactam antibiotics but, also acquiring mechanisms of deacylating these antibiotics. This critical deacylation step leads to release of hydrolyzed and inactivated β-lactams, thereby providing resistance for the bacteria against these antibiotics targeting the cell wall. To combat β-lactamase-mediated antibiotic resistance, numerous β-lactamase inhibitors were developed that utilize various strategies to inactivate the β-lactamase...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29674034/a-voltammetric-test-for-the-rapid-discrimination-of-%C3%AE-lactamase-producing-enterobacteriaceae-in-blood-cultures
#7
Laetitia Betelli, Catherine Neuwirth, Sébastien Solanas, Benoît Chantemesse, Fabienne Vienney, Alain Hartmann, Murielle Rochelet
The accurate identification of β-lactamases produced by Enterobacteriaceae is a major challenge in clinical laboratories in order to optimize antimicrobial treatment and patient care. We describe here a rapid voltammetric-based method to detect and to discriminate β-lactamase activity in Enterobacteriaceae i.e., penicillinase, cephalosporinase (inducible or overproduced), extended-spectrum beta-lactamase and carbapenemase producers. After a 2-h growth step of the sample under three separate conditions: 1) LB (Luria-Bertani) medium, 2) LB supplemented with 4 μg/mL cefotaxime and 3) LB supplemented with 4 μg/mL cefotaxime and 100 μg/mL potassium clavulanate, the β-lactamase activity was measured by incubating a 0...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29672703/phylogenomic-analysis-of-%C3%AE-lactamase-in-archaea-and-bacteria-enables-the-identification-of-putative-new-members
#8
Vivek Keshri, Arup Panda, Anthony Levasseur, Jean-Marc Rolain, Pierre Pontarotti, Didier Raoult
β-lactamases are enzymes which are commonly produced by bacteria and which degrade the β-lactam ring of β-lactam antibiotics, namely penicillins, cephalosporins, carbapenems, and monobactams, and inactivate these antibiotics. We performed a rational and comprehensive investigation of β-lactamases in different biological databases. In this study, we constructed hidden Markov model profiles as well as the ancestral sequence of four classes of β-lactamases (A, B, C, and D), which were used to identify potential β-lactamases from environmental metagenomic (1206), human microbiome metagenomic (6417), human microbiome reference genome (1310), and NCBI's nonredundant databases (44101)...
April 1, 2018: Genome Biology and Evolution
https://www.readbyqxmd.com/read/29671590/selecting-for-fast-protein-protein-association-as-demonstrated-on-a-random-tem1-yeast-library-binding-blip
#9
Ruth Cohen Khait, Gideon Schreiber
Protein-protein interactions mediate the vast majority of cellular processes. Though protein interactions obey basic chemical principles also within the cell, the in vivo physiological environment may not allow for equilibrium to be reached. Thus in vitro measured thermodynamic affinity may not provide a complete picture of protein interactions in the biological context. Binding kinetics composed of the association and dissociation rate constants are relevant and important in the cell. Therefore, changes in protein-protein interaction kinetics have a significant impact on the in vivo activity of the proteins...
April 19, 2018: Biochemistry
https://www.readbyqxmd.com/read/29671219/ceftazidime-avibactam-a-review-in-the-treatment-of-serious-gram-negative-bacterial-infections
#10
Matt Shirley
Ceftazidime-avibactam (Zavicefta® ) is an intravenously administered combination of the third-generation cephalosporin ceftazidime and the novel, non-β-lactam β-lactamase inhibitor avibactam. In the EU, ceftazidime-avibactam is approved for the treatment of adults with complicated urinary tract infections (cUTIs) [including pyelonephritis], complicated intra-abdominal infections (cIAIs), hospital-acquired pneumonia (HAP) [including ventilator-associated pneumonia (VAP)], and other infections caused by aerobic Gram-negative organisms in patients with limited treatment options...
April 18, 2018: Drugs
https://www.readbyqxmd.com/read/29670701/rhodanine-as-a-potent-scaffold-for-the-development-of-broad-spectrum-metallo-%C3%AE-lactamase-inhibitors
#11
Yang Xiang, Cheng Chen, Wen-Ming Wang, Li-Wei Xu, Ke-Wu Yang, Peter Oelschlaeger, Yuan He
A series of rhodanines was constructed, their Z-configuration was confirmed by small molecule X-ray crystal structures, and their activity against metallo-β-lactamases (MβLs) was measured. The obtained 26 molecules and a thioenolate specifically inhibited the MβL L1 with an IC50 range of 0.02-1.7 μM, and compounds 2h - m exhibited broad-spectrum inhibition of the MβLs NDM-1, VIM-2, ImiS, and L1 with IC50 values <16 μM. All inhibitors increased the antimicrobial effect of cefazolin against E. coli cells expressing L1, resulting in a 2-8-fold reduction in MIC...
April 12, 2018: ACS Medicinal Chemistry Letters
https://www.readbyqxmd.com/read/29670677/clinical-outcomes-of-extended-spectrum-beta-lactamase-producing-enterobacteriaceae-infections-with-susceptibilities-among-levofloxacin-cefepime-and-carbapenems
#12
Kristy J Walker, Young R Lee, Amanda R Klar
Purpose: Highly resistant Gram-negative bacterial infections are associated with high mortality. Increasing resistance to standard therapy illustrates the need for alternatives when treating resistant organisms, especially extended-spectrum beta-lactamase- (ESBL-) producing Enterobacteriaceae . Methods: A retrospective chart review at a community hospital was performed. Patients who developed ESBL-producing infections were included. Patients less than eighteen years old, who were pregnant, or who were incarcerated were excluded...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29666727/typhoid-fever-due-to-extended-spectrum-%C3%AE-lactamase-producing-salmonella-enterica-serovar-typhi-a-case-report-and-literature-review
#13
Abdul Azeez Ahamed Riyaaz, Vindya Perera, Sabaratnam Sivakumaran, Nelun de Silva
Emergence of cephalosporin-resistant strains of Salmonella enterica serovar Typhi is a cause of concern in the management of enteric fever. Cephalosporin resistance in Salmonella species is mainly due to the production of extended-spectrum β -lactamases (ESBLs). The majority of ESBLs in Salmonella enterica serovar Typhi are derivatives of the TEM, SHV, and CTX-M β -lactamase families. Of these, CTX-M appears to be predominant. This paper discusses the detection and molecular characterization of an ESBL-producing Salmonella enterica serovar Typhi strain isolated from a patient who was admitted to a private hospital in Sri Lanka...
2018: Case Reports in Infectious Diseases
https://www.readbyqxmd.com/read/29666664/oral-fosfomycin-for-the-treatment-of-acute-and-chronic-bacterial-prostatitis-caused-by-multidrug-resistant-escherichia-coli
#14
REVIEW
George G Zhanel, Michael A Zhanel, James A Karlowsky
Acute and chronic bacterial prostatitis in outpatients is commonly treated with oral fluoroquinolones; however, the worldwide dissemination of multidrug-resistant (MDR) Escherichia coli has resulted in therapeutic failures with fluoroquinolones. We reviewed the literature regarding the use of oral fosfomycin in the treatment of acute and chronic prostatitis caused by MDR E. coli . All English-language references on PubMed from 1986 to June 2017, inclusive, were reviewed from the search "fosfomycin prostatitis...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
https://www.readbyqxmd.com/read/29666548/inhibitory-effect-of-soy-saponins-on-the-activity-of-%C3%AE-lactamases-including-new-delhi-metallo-%C3%AE-lactamase-1
#15
Hitoshi Horie, Asuka Chiba, Shigeo Wada
β-Lactamase-producing bacteria encode enzymes that inactivate β-lactam antibiotics by catalyzing the hydrolysis of the β-lactam ring. Crude soy saponins were observed to have synergistic effects on the antimicrobial activity of β-lactam antibiotics against β-lactamase-producing Staphylococcus aureus strains. Furthermore, the activities of β-lactamases derived from Enterobacter cloacae , Escherichia coli , and S. aureus were decreased significantly in the presence of crude soy saponins. This inhibitory effect was also observed against the New Delhi metallo-β-lactamase 1 (NDM-1), an enzyme whose activity is not inhibited by the current β-lactamase inhibitors...
May 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29665885/prevalence-of-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-and-residual-antimicrobials-in-the-environment-in-vietnam
#16
Shinji Yamasaki, Tuyen Danh Le, Mai Quang Vien, Chinh Van Dang, Yoshimasa Yamamoto
Emergence and spread of antimicrobial-resistant bacteria, including extended-spectrum β-lactamase (ESBL)-producing Escherichia coli, have become serious problems worldwide. Recent studies conducted in Vietnam revealed that ESBL-producing E. coli are widely distributed in food animals and people. CTX-M-9 and CTX-M-1 are the most prevalent β-lactamases among the identified ESBLs. Furthermore, most of the ESBL-producing E. coli isolates were multi-drug resistant. Residual antimicrobials such as sulfamethoxazole, trimethoprim, sulfadimidine, cephalexin, and sulfadiazine were also detected at a high level in both animal meats and environmental water collected from several cities, including Ho Chi Minh city and Can Tho city...
December 2017: Animal Health Research Reviews
https://www.readbyqxmd.com/read/29665444/ndm-1-and-oxa-23-producing-acinetobacter-baumannii-isolated-from-intensive-care-unit-patients-in-tunisia
#17
Elaa Maamar, Carla Andrea Alonso, Sana Ferjani, Ali Jendoubi, Zaineb Hamzaoui, Alia Jebri, Mabrouka Saidani, Salma Ghedira, Carmen Torres, Ilhem Boutiba-Ben Boubaker
Gastrointestinal colonization by carbapenem-resistant Acinetobacter baumannii (CRAB), is a critical step before nosocomial infection. Our objective was to evaluate CRAB intestinal carriage in patients admitted in the intensive care unit (ICU) of a Tunisian Hospital and to determine the antimicrobial resistance mechanisms involved. From December-2014 to February-2015, all 63 patients admitted at the ICU were screened for rectal CRAB-colonization upon admission and once weekly thereafter. ICU-patients who acquired a CRAB-nosocomial-infection were also included...
April 14, 2018: International Journal of Antimicrobial Agents
https://www.readbyqxmd.com/read/29664687/distinctiveness-and-similarities-between-extended-spectrum-%C3%AE-lactamase-producing-escherichia-coli-isolated-from-cattle-and-the-community-in-israel
#18
Ziv Lifshitz, Na'ama Sturlesi, Miriam Parizade, Shlomo E Blum, Michal Gordon, Diana Taran, Amos Adler
The goal of this study was to compare the molecular features of bovine- and human community-acquired extended-spectrum β-lactamase (ESBL)-producing Escherichia coli in Israel. Bovine ESBL-producing E. coli were isolated during a point-prevalence study from the main farming locations throughout Israel. Human ESBL-producing E. coli isolates were collected from community-acquired urinary tract infection cases. Molecular typing was done initially by repetitive extragenic palindromic-PCR. Representative isolates were subjected to next-generation sequencing (NGS) and analyzed for multilocus sequence typing (MLST), core genome MLST (cgMLST), blaCTX-M gene allele, and mobile genetic elements (MGEs) surrounding it...
April 17, 2018: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/29663096/plasmids-carrying-dha-1-%C3%AE-lactamases
#19
REVIEW
Claire Hennequin, Viviane Ravet, Frédéric Robin
The aim of this review is to provide an update on the plasmids mediating DHA-1 cephalosporinase in Klebsiella pneumoniae. These plasmids have been mainly found in this bacterium but not only. The first was isolated from Salmonella sp. in France in the early 1990s. They are currently reported worldwide. BlaDHA-1 beta-lactamase gene is usually co-expressed with many other antibiotic resistance genes such as extended-spectrum β-lactamases (blaCTX-M -, bla SHV -types), oxacillinases (blaOXA-1, blaOXA-30 ), penicillinases (bla TEM -type), carbapenemases (bla OXA48 , blaKPC-2 ), aminoglycosides (aacA, aadA, armA), fluoroquinolones (qnrB4, aac6'-1b-cr), and sulfonamide (sul1) resistance genes...
April 17, 2018: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/29663045/infection-related-ventilator-associated-complications-in-icu-patients-colonised-with-extended-spectrum-%C3%AE-lactamase-producing-enterobacteriaceae
#20
François Barbier, Sébastien Bailly, Carole Schwebel, Laurent Papazian, Élie Azoulay, Hatem Kallel, Shidasp Siami, Laurent Argaud, Guillaume Marcotte, Benoît Misset, Jean Reignier, Michaël Darmon, Jean-Ralph Zahar, Dany Goldgran-Toledano, Étienne de Montmollin, Bertrand Souweine, Bruno Mourvillier, Jean-François Timsit
PURPOSE: To investigate the clinical significance of infection-related ventilator-associated complications (IVAC) and their impact on carbapenem consumption in mechanically ventilated (MV) patients colonised with extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBLE). METHODS: Inception cohort study from the French prospective multicenter OUTCOMEREA database (17 ICUs, 1997-2015) including all ESBLE carriers (systematic rectal swabbing at admission then weekly and/or urinary or superficial surgical site colonisation) with MV duration > 48 h and ≥ 1 episode of IVAC after carriage documentation...
April 16, 2018: Intensive Care Medicine
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