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Enzyme immobilization

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https://www.readbyqxmd.com/read/28094405/controlled-and-tunable-design-of-polymer-interface-for-immobilization-of-enzymes-does-curvature-matter
#1
Claudia Marschelke, Ivan Raguzin, Anke Matura, Andreas Fery, Alla Synytska
Control and tuning of surface properties is indispensable for the programmed and rational design of materials. Particularly, polymeric brush-modified colloids can be used as carrier materials for enzyme immobilization. Although it is of prime importance to control the brush architecture, there is still a lack of systematic investigations concerning the impact of grafting density on the properties of the designed interface, as well as on the immobilization of biomolecules. In this work, we investigate the surface properties of polymer brushes with different grafting densities prepared using a "grafting from" approach on flat and on colloidal particle substrates by varying the density of initiator groups...
January 17, 2017: Soft Matter
https://www.readbyqxmd.com/read/28093127/magnetic-beads-based-dna-hybridization-chain-reaction-amplification-and-dnazyme-recognition-for-colorimetric-detection-of-uranyl-ion-in-seafood
#2
Hongyan Zhang, Xian Cheng, Lian Chen, Fan Mo, LiangJun Xu, FengFu Fu
A novel colorimetric biosensor, which employs DNAzyme-functionalized magnetic beads (MBs) as recognition probe, enzyme-assisted catalytic oxidation of TMB (3,3',5,5'-tetramethylbenzidine sulfate) as signal and DNA hybridization chain reaction as amplification strategy, has been developed for detecting trace uranyl ion (UO2(2+)) in seafood and aqueous environment with high sensitivity and specificity. We demonstrated that UO2(2+) can specifically cleave DNAzyme immobilized on MBs surface to release a short single-strand DNA (primer), and the released primer trigger DNA hybridization chain reaction to form a long one dimensional DNA concatamer on the MBs surface...
March 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28089928/improved-catalytic-properties-of-penicillium-notatum-lipase-immobilized-in-nanoscale-silicone-polymeric-films
#3
Saima Rehman, Ping Wang, Haq Nawaz Bhatti, Muhammad Bilal, Muhammad Asgher
Lipases are one of the most proficient biocatalysts having enormous biotechnological prospective. Immobilization offers a potential solution to improve the stability and recycling characteristics of lipases. An extracellular lipase from Penicillium notatum (PNL) was immobilized in silicon polymers (SiP) through entrapment, and subsequently coated this matrix on the network of fibers in the sponges. The silicone polymers-immobilized lipase (SiP-lipase) displayed highest apparent activity and entrapment efficiency of 1...
January 12, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28088750/continuous-minimally-invasive-alcohol-monitoring-using-microneedle-sensor-arrays
#4
A M Vinu Mohan, Joshua Ray Windmiller, Rupesh K Mishra, Joseph Wang
The present work describes an attractive skin-worn microneedle sensing device for the minimally invasive electrochemical monitoring of subcutaneous alcohol. The device consists of an assembly of pyramidal microneedle structures integrated with Pt and Ag wires, each with a microcavity opening. The microneedle aperture was modified by electropolymerizing o-phenylene diamine onto the Pt wire microtransducer, followed by the immobilization of alcohol oxidase (AOx) in an intermediate chitosan layer, along with an outer Nafion layer...
January 10, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28087528/engineering-mycobacteria-for-the-production-of-self-assembling-biopolyesters-displaying-mycobacterial-antigens-for-use-as-tuberculosis-vaccine
#5
Jason W Lee, Natalie A Parlane, Bernd H A Rehm, Bryce M Buddle, Axel Heiser
: Tuberculosis (TB) is a disease caused by Mycobacterium tuberculosis or Mycobacterium bovis and still remains one of the world's biggest global health burdens. Recently, engineered polyhydroxyalkanoate (PHA) biobeads produced in both E. coli and Lactococcus lactis displaying mycobacterial antigens were found to induce significant cell mediated immune responses in mice. We observed that such PHA beads contained host cell proteins as impurities which we hypothesized to have the potential to induce immunity...
January 13, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28080020/biocatalytic-self-assembly-using-reversible-and-irreversible-enzyme-immobilization
#6
Maria Paola Conte, King Hang Aaron Lau, Rein V Ulijn
Biocatalytic control of molecular self-assembly provides an effective approach for developing smart biomaterials, allowing versatile enzyme-mediated tuning of material structure and properties as well as biomedical applications. We functionalized surfaces with bioinspired polydopamine and polyphenol coatings to study the effects of enzyme surface localization and surface release on the self-assembly process. We show how these coatings could be conveniently used to release enzymes for bulk gelation as well as to irreversibly immobilize enzymes for localizing the self-assembly to the surface...
January 12, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28078719/carbon-nanotube-supported-bio-inspired-nickel-catalyst-and-its-integration-in-hybrid-hydrogen-air-fuel-cells
#7
Solène Gentil, Noémie Lalaoui, Arnab Dutta, Yannig Nedellec, Serge Cosnier, Wendy J Shaw, Vincent Artero, Alan Le Goff
A biomimetic nickel bis-diphosphine complex incorporating the amino acid arginine in the outer coordination sphere was immobilized on modified carbon nanotubes (CNTs) through electrostatic interactions. The functionalized redox nanomaterial exhibits reversible electrocatalytic activity for the H2 /2 H(+) interconversion from pH 0 to 9, with catalytic preference for H2 oxidation at all pH values. The high activity of the complex over a wide pH range allows us to integrate this bio-inspired nanomaterial either in an enzymatic fuel cell together with a multicopper oxidase at the cathode, or in a proton exchange membrane fuel cell (PEMFC) using Pt/C at the cathode...
January 12, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28078652/free-and-ca-alginate-beads-immobilized-horseradish-peroxidase-for-the-removal-of-reactive-dyes-an-experimental-and-modeling-study
#8
Simone Farias, Diego A Mayer, Débora de Oliveira, Selene M A Guelli U de Souza, Antônio Augusto Ulson de Souza
The aim of this work was to remove the dyes Reactive Blue 221 (RB 221) and Reactive Blue 198 (RB 198) of synthetic effluent using the immobilized enzyme horseradish peroxidase (HRP) in Ca-alginate beads. Experimental parameters affecting the dye removal process such as the effect of pH, temperature, hydrogen peroxide concentration, mass capsules, and reuse were evaluated, and a numerical model of mass transfer was developed. A maximum removal of 93 and 75%, respectively, for the dyes RB 221 and RB 198, at pH 5...
January 11, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28078417/covalent-organic-framework-coated-magnetic-graphene-as-a-novel-support-for-trypsin-immobilization
#9
Heping Wang, Fenglong Jiao, Fangyuan Gao, Xinyuan Zhao, Yan Zhao, Yehua Shen, Yangjun Zhang, Xiaohong Qian
Deep and efficient proteolysis is the critical premise in mass spectrometry-based bottom-up proteomics. It is difficult for traditional in-solution digestion to meet the requirement unless prolonged digestion time and enhanced enzyme dosage are employed, which makes the whole workflow time-consuming and costly. The abovementioned problems could be effectively ameliorated by anchoring many proteases on solid supports. In this work, covalent organic framework-coated magnetic graphene (MG@TpPa-1) was designed and prepared as a novel enzyme carrier for the covalent immobilization of trypsin with a high degree of loading (up to 268 μg mg(-1))...
January 11, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28070779/hydrolytic-activity-of-esterase-antibody-complexes-retained-within-gel-capsules-after-complex-isolation
#10
Youji Shimazaki, Rino Miyatsuka
Delipidation in biological samples is important for some diagnostic tests and protein analyses. Lipids in the samples can be hydrolyzed by native esterases (ESs) within gel capsules after ES, and ES-antibody complexes are specifically trapped, extracted, and separated. Acrylamide and agarose gel capsules containing complexes of ES antibody were produced after the complexes were extracted using protein A-immobilized membranes, separated by non-denaturing electrophoresis, and stained by colloidal silver using glucose as a reductant...
January 9, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28069495/surface-modification-of-copolymerized-films-from-three-armed-biodegradable-macromers-an-analytical-platform-for-modified-tissue-engineering-scaffolds
#11
Benno M Müller, Rudi Loth, Peter-Georg Hoffmeister, Friederike Zühl, Liv Kalbitzer, Michael C Hacker, Michaela Schulz-Siegmund
: The concept of macromers allows for a broad adjustment of biomaterial properties by macromer chemistry or copolymerization. Copolymerization strategies can also be used to introduce reactive sites for subsequent surface modification. Control over surface features enables adjustment of cellular reactions with regard to site and object of implantation. We designed macromer-derived polymer films which function as non-implantable analytical substrates for the investigation of surface properties of equally composed scaffolds for bone tissue engineering...
January 6, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28069208/ultrasound-pretreatment-as-a-novel-approach-for-intensification-of-lipase-catalyzed-esterification-of-tricaprylin
#12
Snehal B More, Jyotsna T Waghmare, Parag R Gogate
The current work deals with ultrasound assisted intensification of synthesis of tricaprylin based on the enzyme catalyzed reaction of caprylic acid and glycerol with novel approach of using ultrasound in only the initial stages of the reaction. Two types of immobilized lipases as Lipozyme RM (Rhizomucor miehei) and Novozym 435 (Candida antarctica) have been used in the work. The effect of ultrasonic conditions such as treatment time and power as well as the reaction conditions such as substrate molar ratio, reaction time and enzyme loading on the yield of tricaprylin has been investigated...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28069187/characteristics-of-pectinase-treated-with-ultrasound-both-during-and-after-the-immobilization-process
#13
Xiaobin Ma, Danli Wang, Michelle Yin, Juliet Lucente, Wenjun Wang, Tian Ding, Xingqian Ye, Donghong Liu
In this study, ultrasound was applied both during and after the immobilization process and characteristics of different immobilized pectinase samples were studied. When introduced during the immobilization process, ultrasound at an intensity of 9WmL(-1) for 20min increased the immobilization yield 92.28% more than the control. When introduced to the already immobilized pectinase, ultrasound at an intensity of 4.5WmL(-1) for 10min increased the pectinase activity by 30.05%. Results of scanning electron microscope demonstrated that ultrasound increased surface area and loosened structures of immobilized enzymes...
May 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28068396/effects-of-pulp-and-na-bentonite-amendments-on-the-mobility-of-trace-elements-soil-enzymes-activity-and-microbial-parameters-under-ex-situ-aided-phytostabilization
#14
Daniel Wasilkowski, Anna Nowak, Grażyna Płaza, Agnieszka Mrozik
The objective of this study was to explore the potential use of pulp (by-product) from coffee processing and Na-bentonite (commercial product) for minimizing the environmental risk of Zn, Pb and Cd in soil collected from a former mine and zinc-lead smelter. The effects of soil amendments on the physicochemical properties of soil, the structural and functional diversity of the soil microbiome as well as soil enzymes were investigated. Moreover, biomass of Festuca arundinacea Schreb. (cultivar Asterix) and the uptake of trace elements in plant tissues were studied...
2017: PloS One
https://www.readbyqxmd.com/read/28067789/desorption-of-lipases-immobilized-on-octyl-agarose-beads-and-coated-with-ionic-polymers-after-thermal-inactivation-stronger-adsorption-of-polymers-unfolded-protein-composites
#15
Jose J Virgen-Ortíz, Sara G Pedrero, Laura Fernandez-Lopez, Nerea Lopez-Carrobles, Beatriz C Gorines, Cristina Otero, Roberto Fernandez-Lafuente
Lipases from Candida antarctica (isoform B) and Rhizomucor miehei (CALB and RML) have been immobilized on octyl-agarose (OC) and further coated with polyethylenimine (PEI) and dextran sulfate (DS). The enzymes just immobilized on OC supports could be easily released from the support using 2% SDS at pH 7, both intact or after thermal inactivation (in fact, after inactivation most enzyme molecules were already desorbed). The coating with PEI and DS greatly reduced the enzyme release during thermal inactivation and improved enzyme stability...
January 5, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28065752/covalent-immobilization-of-trypsin-onto-modified-magnetite-nanoparticles-and-its-application-for-casein-digestion
#16
Keziban Atacan, Bekir Çakıroğlu, Mahmut Özacar
The immobilization method consists of the production magnetite nanoparticles (Fe3O4) by solvothermal treatment of FeCl3 and sodium acetate (NaAc) in the presence of ethylene glycol. Subsequently, the surface of magnetite nanoparticles was modified with a well-known polyphenol tannic acid. Trypsin was covalently immobilized on the tannic acid modified magnetite nanoparticles after exposing the modified nanoparticles to pH 9.4. Then, tryptic digestion of casein by free and immobilized trypsin was carried out for 13h and 1h, respectively...
January 5, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28064428/phospholipase-a1-catalysed-synthesis-of-docosahexaenoic-acid-enriched-phosphatidylcholine-in-reverse-micelles-system
#17
Wuxi Chen, Wei Guo, Feng Gao, Limei Chen, Shulin Chen, Demao Li
Phosphatidylcholine enriched with docosahexaenoic acid (DHA) was successfully produced by phospholipase A1-catalysed acidolysis. Reverse micelles were firstly selected as the reaction system to avoid the process of immobilization and to ensure the efficient catalysis of phospholipase A1. Parameters were optimized for a high incorporation of phosphatidylcholine enriched with DHA (DHA-PC). A response-surface design with four factors, including the water content, enzyme loading, pH and substrate-mass ratio were used to evaluate the influence of the major factors and to predict the optimal reaction conditions...
January 7, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28064425/enantioselective-resolution-of-r-s-carvedilol-to-s-carvedilol-by-biocatalysts
#18
Swetha Ettireddy, Vijitha Chandupatla, Ciddi Veeresham
Among the microorganisms employed in the study, Aspergillus niger (GUFCC5443), Escherichia coli (ATCC9637), Streptomyces halstedii (CKM-2), Pseudomonas putida (NCIB9494), Cunninghamella elegans (NCIM689) and Sphingomonas paucimobilis (NCTC11030) were capable for the enantioselective conversion of racemic Carvedilol. Immobilization technique enhanced the enantioselectivity of microorganisms and thus increased the enantiomeric purity of the drug. Excellent enantiomeric ratios (E) were found in reactions catalyzed by immobilized A...
January 7, 2017: Natural Products and Bioprospecting
https://www.readbyqxmd.com/read/28064043/conductive-hydrogel-composed-of-1-3-5-benzenetricarboxylic-acid-and-fe-3-used-as-enhanced-electrochemical-immunosensing-substrate-for-tumor-biomarker
#19
Huiqiang Wang, Hongliang Han, Zhanfang Ma
In this work, a new conductive hydrogel was prepared by a simple cross-linking coordination method using 1,3,5-benzenetricarboxylic acid as the ligand and Fe(3+) as the metal ion. The hydrogel film was formed on a glassy carbon electrode (GCE) by a drop coating method, which can dramatically facilitate the transport of electrons. A sensitive label-free electrochemical immunosensor was fabricated following electrodeposition of gold nanoparticles (AuNPs) on a hydrogel film and immobilization of an antibody. Neuron-specific enolase (NSE), a lung cancer biomarker, was used as the model analyte to be detected...
December 30, 2016: Bioelectrochemistry
https://www.readbyqxmd.com/read/28063621/biodegradation-of-carbon-nanotubes-graphene-and-their-derivatives
#20
REVIEW
Ming Chen, Xiaosheng Qin, Guangming Zeng
Carbon nanotubes (CNTs), graphene (GRA), and their derivatives are promising materials for a wide range of applications such as pollutant removal, enzyme immobilization, bioimaging, biosensors, and drug delivery and are rapidly increasing in use and increasingly mass produced. The biodegradation of carbon nanomaterials by microbes and enzymes is now of great importance for both reducing their toxicity to living organisms and removing them from the environment. Here we review recent progress in the biodegradation field from the point of view of the primary microbes and enzymes that can degrade these nanomaterials, along with experimental and molecular simulation methods for the exploration of nanomaterial degradation...
January 4, 2017: Trends in Biotechnology
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