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Enzyme immobilization

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https://www.readbyqxmd.com/read/29334175/efficient-catalytic-oxidation-of-5-hydroxymethylfurfural-to-2-5-furandicarboxylic-acid-by-magnetic-laccase-catalyst
#1
Ke-Feng Wang, Chun-Lei Liu, Kun-Yan Sui, Chen Guo, Chun-Zhao Liu
2,5-Furandicarboxylic acid (FDCA) is a biobased platform chemical for the production of polyesters. The magnetic laccase catalyst with TEMPO as the mediator has the remarkable capability of oxidizing HMF to 2,5-furandicarboxylic acid (FDCA). Under optimal reaction conditions, a quantitative yield (90.2%) of FDCA with complete HMF conversion was obtained after 96 h of reaction. More importantly, the magnetic laccase catalyst exhibited good recyclability and stability, maintaining 84.8% of its original activity following 6 reuse cycles...
January 15, 2018: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/29333698/design-of-enzyme-micelles-with-controllable-concavo-convex-micromorphologies-for-highly-enhanced-stability-and-catalytical-activity
#2
Tao Chen, Yuanhong Xu, Wenrong Yang, Aihua Li, Yao Wang, Jing Sun, Jingquan Liu
Concavo-convex micelles with controllable sizes and nanostructures are prepared via self-assembling polymer-enzyme (e.g., shellac enzyme) conjugates with heterogeneous polymer chains, which exhibit higher enzyme stability (300%) and bioactivity (760%) comparing with the well-defined ones. The applied amphiphilic and negatively charged copolymer, poly (methyl methacrylate)-block-poly (sodium p-styrene sulfonate), is synthesized via reversible addition-fragmentation chain transfer polymerization to modify shellac enzyme and immobilize the enzyme bioactivity inducer by covalent conjugation and electrostatic attraction, respectively...
January 15, 2018: Macromolecular Bioscience
https://www.readbyqxmd.com/read/29332820/a-strategy-for-screening-of-%C3%AE-glucosidase-inhibitors-from-morus-alba-root-bark-based-on-the-ligand-fishing-combined-with-high-performance-liquid-chromatography-mass-spectrometer-and-molecular-docking
#3
Zhen Wang, Xiaoqing Li, Menghan Chen, Feiyan Liu, Chao Han, Lingyi Kong, Jianguang Luo
A new method based on ligand fishing combined with high-performance liquid chromatography quadrupole-time-of-flight mass spectrometer and molecular docking was established to screen α-glucosidase inhibitors from a traditional Chinese medicine Morus alba root bark. α-Glucosidase was immobilized on magnetic nanoparticles, used as a solid support to incubate with crude extract. After ligand fishing, the eluates were analyzed by high-performance liquid chromatography quadrupole-time-of-flight mass spectrometer, obtaining eleven ligands (1-4, 6-12) eventually...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29332803/particle-based-immobilized-enzymatic-reactors-in-microfluidic-chips
#4
REVIEW
Adam Kecskemeti, Attila Gaspar
The research and applications of immobilized enzyme reactors (IMERs) have become more and more widespread due to the numerous advantages like reusability, easy handling, prolonged lifetime, easy separation from products and substrate specificity. The miniaturized form of these reactors (microchip IMERs) received outstanding attention due to their special features and advantages over the traditional, larger analytical systems. Large specific surface is essential for the efficient operation of the microreactors, thus these devices include one of the several types of porous solid supports, but in this work only the particle based microchip IMERs are reviewed...
April 1, 2018: Talanta
https://www.readbyqxmd.com/read/29331825/a-novel-strategy-for-the-efficient-removal-of-toxic-cyanate-by-the-combinatorial-use-of-recombinant-enzymes-immobilized-on-aminosilane-modified-magnetic-nanoparticles
#5
Bibhuti Ranjan, Santhosh Pillai, Kugenthiren Permaul, Suren Singh
Cyanase detoxifies cyanate by transforming it to ammonia and carbon dioxide in a bicarbonate-dependent reaction, however, dependence on bicarbonate limits its utilization in large-scale applications. A novel strategy was therefore developed for overcoming this bottleneck by the combined application of cyanase (rTl-Cyn) and carbonic anhydrase (rTl-CA). The synergistic effect of rTl-Cyn and rTl-CA could reduce the dependence of bicarbonate by 80%, compared to using rTl-Cyn alone. Complete degradation of cyanate (4 mM) was achieved with buffered conditions and 85 ± 5% degradation with industrial wastewater sample, when 20 U of rTl-Cyn was applied...
December 29, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/29329004/contemporary-enzyme-based-technologies-for-bioremediation-a-review
#6
REVIEW
Babita Sharma, Arun Kumar Dangi, Pratyoosh Shukla
The persistent disposal of xenobiotic compounds like insecticides, pesticides, fertilizers, plastics and other hydrocarbon containing substances is the major source of environmental pollution which needs to be eliminated. Many contemporary remediation methods such as physical, chemical and biological are currently being used, but they are not sufficient to clean the environment. The enzyme based bioremediation is an easy, quick, eco-friendly and socially acceptable approach used for the bioremediation of these recalcitrant xenobiotic compounds from the natural environment...
January 9, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29324678/immobilization-of-enzymes-on-a-phospholipid-bionically-modified-polysulfone-gradient-pore-membrane-for-the-enhanced-performance-of-enzymatic-membrane-bioreactors
#7
Yizong Guo, Xueyan Zhu, Fei Fang, Xiao Hong, Huimin Wu, Dajing Chen, Xiaojun Huang
Enzymatic membrane bioreactors (EMBRs), with synergistic catalysis-separation performance, have increasingly been used for practical applications. Generally, the membrane properties, particularly the pore structures and interface interactions, have a significant impact on the catalytic efficiency of the EMBR. Therefore, a biomimetic interface based on a phospholipid assembled onto a polysulfone hollow-fiber membrane with perfect radial gradient pores (RGM-PSF) has been prepared in this work to construct a highly efficient and stable EMBR...
January 11, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29323888/covalent-immobilization-of-cellulase-using-magnetic-poly-ionic-liquid-support-improvement-of-the-enzyme-activity-and-stability
#8
Seyed Hassan Hosseini, Seyedeh Ameneh Hosseini, Nasrin Zohreh, Mahshid Yaghoubi, Ali Pourjavadi
A magnetic nanocomposite was prepared by entrapment of Fe3O4 nanoparticles into the cross-linked ionic liquid/epoxy type polymer. The resulting support was used for covalent immobilization cellulase through the reaction with epoxy groups. The ionic surface of the support improved the adsorption of enzyme and a large amount of enzyme (106.1 mg/g) was loaded onto the support surface. The effect of the presence of ionic monomer and covalent binding of enzyme was also investigated. The structures of supports were characterized by various instruments such as FT-IR, TGA, VSM, XRD, TEM, SEM and DLS...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29323458/metal-organic-framework-encapsulation-for-the-preservation-and-photothermal-enhancement-of-enzyme-activity
#9
Sirimuvva Tadepalli, Jieun Yim, Sisi Cao, Zheyu Wang, Rajesh R Naik, Srikanth Singamaneni
Interfacing biomolecules with functional materials is a key strategy toward achieving externally-triggered biological function. The rational integration of functional proteins, such as enzymes, with plasmonic nanostructures that exhibit unique optical properties such as photothermal effect provides a means to externally control the enzyme activity. However, due to the labile nature of enzymes, the photothermal effect of plasmonic nanostructures is mostly utilized for the enhancement of the biocatalytic activity of thermophilic enzymes...
January 11, 2018: Small
https://www.readbyqxmd.com/read/29322650/molecular-characterization-of-the-%C3%AE-amyloid-4-10-epitope-of-plaque-specific-a%C3%AE-antibodies-by-affinity-mass-spectrometry-using-alanine-site-mutation
#10
Raluca Ștefănescu, Loredana Lupu, Marilena Manea, Roxana E Iacob, Michael Przybylski
Alzheimer disease is a neurodegenerative disease affecting an increasing number of patients worldwide. Current therapeutic strategies are directed to molecules capable to block the aggregation of the β-amyloid(1-42) (Aβ) peptide and its shorter naturally occurring peptide fragments into toxic oligomers and amyloid fibrils. Aβ-specific antibodies have been recently developed as powerful antiaggregation tools. The identification and functional characterization of the epitope structures of Aβ antibodies contributes to the elucidation of their mechanism of action in the human organism...
January 2018: Journal of Peptide Science: An Official Publication of the European Peptide Society
https://www.readbyqxmd.com/read/29322108/production-of-biodiesel-by-enzymatic-transesterification-of-waste-sardine-oil-and-evaluation-of-its-engine-performance
#11
A Arumugam, V Ponnusami
Waste sardine oil, a byproduct of fish industry, was employed as a low cost feedstock for biodiesel production. It has relatively high free fatty acid (FFA) content (32 mg KOH/g of oil). Lipase enzyme immobilized on activated carbon was used as the catalyst for the transesterification reaction. Process variables viz. reaction temperature, water content and oil to methanol molar ratio were optimized. Optimum methanol to oil molar ratio, water content and temperature were found to be 9:1, 10 v/v% and 30 °C respectively...
December 2017: Heliyon
https://www.readbyqxmd.com/read/29321979/improvement-of-aspergillus-flavus-saponin-hydrolase-thermal-stability-and-productivity-via-immobilization-on-a-novel-carrier-based-on-sugarcane-bagasse
#12
Hala A Amin, Francesco Secundo, Hassan Amer, Faten A Mostafa, Wafaa A Helmy
Soyasapogenol B (SB) is known to have many biological activities such as hepatoprotective, anti-inflammatory, anti-mutagenic, antiviral and anticancer activities. Enzymatic conversion of soyasaponins to SB was carried out using saponin hydrolase (SH) extracted from Aspergillus flavus. The partially purified enzyme was immobilized on different carriers by physical adsorption, covalent binding or entrapment. Among the investigated carriers, Eupergit C and sugarcane bagasse (SCB) activated by DIC and NHS were the most suitable two carriers for immobilization (the immobilized forms recovered 46...
March 2018: Biotechnology Reports
https://www.readbyqxmd.com/read/29319001/vertically-grown-zinc-oxide-nanorods-functionalized-with-ferric-oxide-for-in-vivo-and-non-enzymatic-glucose-detection
#13
Mohammed Marie, Anishkumar Manoharan, Andrian V Kuchuk, Simon Ang, M Omar Manasreh
An enzyme-free glucose sensor out of vertically grown Zinc oxide nanorods (ZnO NRs) functionalized with ferric oxide Fe2O3 is investigated. The well-aligned and high-dense ZnO NRs were synthesized on an FTO/glass substrate by sol-gel and hydrothermal growth methods. Dip-coating technique was utilized to modify the surface of the as-grown ZnO NRs with Fe2O3, and the immobilized surface was coated with a layer of a nafion membrane. The fabricated glucose sensor was characterized amperometrically using three electrodes station at room temperature in the phosphate buffer solution, where ZnO NRs/Fe2O3/nafion membrane was the sensing or working electrode, platinum plate and silver/silver chloride were the counter and the reference electrodes respectively...
January 10, 2018: Nanotechnology
https://www.readbyqxmd.com/read/29318883/colorimetric-and-electrochemiluminecence-dual-mode-sensing-of-lead-ion-based-on-integrated-lab-on-paper-device
#14
Jinmeng Xu, Yan Zhang, Li Li, Qingkun Kong, Lina Zhang, Shenguang Ge, Jinghua Yu
A highly selective two-point separation strategy was designed based on a cross-like all-in-one lab-on-paper analytical device. The stable and cleavable enzyme-coated reduced graphene oxide (rGO)-PdAu probe was fabricated as the signal reporter to enable visualization and electrochemiluminecence (ECL) dual mode sensing of Pb2+. Concretely, the experimental workflow consists of the following process, (i) fabrication of the lab-on-paper device and growth of Au NPs on ECL detection zone, (ii) immobilization of Pb2+-specific DNAzyme, (iii) hybridization between DNAzyme and rGO-PdAu-glucose oxidase (GOx) labeled oligonucleotide to form double-stranded DNA...
January 10, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29317354/laccase-catalyzed-elimination-of-morphine-from-aqueous-systems
#15
Daniela Huber, Klaus Bleymaier, Alessandro Pellis, Robert Vielnascher, Andreas Daxbacher, Katrin J Greimel, Georg M Guebitz
Pharmaceuticals contaminate the environment for several reasons, including metabolic excretion after intake, industrial waste and improper disposal. The narcotic drug morphine is commonly utilized for chronic pain management, and the distribution of morphine in aqueous systems and in waste waters is of high concern. Here. the removal of morphine by a laccase from Myceliophthora thermophila both in its free form as well as immobilized on Accurel MP1000 beads was investigated. Complete morphine elimination was achieved within 30 min for the free and the immobilized enzyme (70% bound protein) for concentrations between 1 and 1,000 mg L-1 according to LC-TOF mass spectrometry analysis...
January 6, 2018: New Biotechnology
https://www.readbyqxmd.com/read/29316370/physicochemical-and-catalytic-properties-of-acylase-i-from-aspergillus-melleus-immobilized-on-amino-and-carbonyl-grafted-st%C3%A3-ber-silica
#16
Agnieszka Kołodziejczak-Radzimska, Jakub Zdarta, Teofil Jesionowski
Acylase I from Aspergillus melleus was immobilized on supports consisting of unmodified and modified silica. Modification was performed using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (GA). The effectiveness of immobilization was investigated using the standard Bradford method in addition to a number of physicochemical techniques, including spectroscopic methods (FTIR, 29 Si and 13 C CP MAS NMR), porous structure and elemental analysis, and zeta potential measurement. A determination of catalytic activity was made based on the deacetylation reaction of N-acetyl-l-methionine...
January 8, 2018: Biotechnology Progress
https://www.readbyqxmd.com/read/29316144/quantitative-comparison-of-enzyme-immobilization-strategies-for-glucose-biosensing-in-real-time-using-fast-scan-cyclic-voltammetry-coupled-with-carbon-fiber-microelectrodes
#17
Samantha K Smith, Leyda Z Lugo-Morales, Christina Tang, Saahj P Gosrani, Christie A Lee, James G Roberts, Stephen W Morton, Gregory S McCarty, Saad A Khan, Leslie A Sombers
Electrochemical monitoring of non-electroactive species requires a biosensor that is stable and selective, with sensitivity to physiological concentrations of targeted analytes. We have combined glucose oxidase-modified carbon-fiber microelectrodes with fast-scan cyclic voltammetry for real-time measurements of glucose fluctuations in brain tissue. Work presented herein quantitatively compares three approaches to enzyme immobilization on the microelectrode surface - physical adsorption, hydrogel entrapment, and entrapment in electrospun nanofibers...
January 9, 2018: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
https://www.readbyqxmd.com/read/29315710/an-improved-design-to-capture-magnetic-microparticles-for-capillary-electrophoresis-based-immobilized-microenzyme-reactors
#18
Pranov Ramana, Jan Schejbal, Kristof Houthoofd, Johan Martens, Erwin Adams, Patrick Augustijns, Zdenĕk Glatz, Ann Van Schepdael
In this paper, we demonstrate the effectiveness of a new 3D printed magnet holder that enables capture of magnetic microparticles in commercially available capillary electrophoresis equipment with a liquid or air based coolant system. The design as well as the method to capture magnetic microparticles inside the capillary are discussed. This setup was tested at temperature and pH values suitable for performing enzymatic reactions. To demonstrate its applicability in CE- immobilized microenzyme reactors (IMER) development, human flavin-containing monooxygenase 3 and bovine serum albumin were immobilized on amino functionalized magnetic microparticles using glutaraldehyde...
January 5, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29313333/dithiothreitol-regulated-coverage-of-oligonucleotide-modified-gold-nanoparticles-to-achieve-optimized-biosensor-performance
#19
Pingping Liang, Juan Canoura, Haixiang Yu, Obtin Alkhamis, Yi Xiao
DNA-modified gold nanoparticles (AuNPs) are useful signal-reporters for the detection of a variety of molecules via different hybridization- and enzyme-based assays. However, their performance is heavily dependent on the surface coverage of the immobilized probe DNA, which can influence both target binding and enzymatic processing of the bound probes. Current methods used to adjust DNA surface coverage on AuNPs require the production of multiple batches of AuNPs under different conditions, which is costly and laborious...
January 9, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29310854/stabilization-of-dimeric-%C3%AE-glucosidase-from-aspergillus-niger-via-glutaraldehyde-immobilization-under-different-conditions
#20
Perla Guadalupe Vazquez-Ortega, Maria Teresa Alcaraz-Fructuoso, Juan A Rojas-Contreras, Javier López-Miranda, Roberto Fernandez-Lafuente
The dimeric enzyme β-glucosidase from Aspergillus niger has been immobilized on different amino-agarose beads at pH 5 and 7, exploiting the versatility of glutaraldehyde. The stability of the free enzyme depended on enzyme concentration. Immobilization via ion exchange improved enzyme stability/activity, depending on the immobilization pH. However, the enzyme was desorbed in 75 mM NaCl at pH 7 and some stability/enzyme concentration dependence still existed. TREATMENT: of these biocatalysts with glutaraldehyde increased enzyme stability (e...
March 2018: Enzyme and Microbial Technology
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