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Enzyme immobilization

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https://www.readbyqxmd.com/read/28231504/effect-of-biochar-on-cadmium-bioavailability-and-uptake-in-wheat-triticum-aestivum-l-grown-in-a-soil-with-aged-contamination
#1
Tahir Abbas, Muhammad Rizwan, Shafaqat Ali, Muhammad Zia-Ur-Rehman, Muhammad Farooq Qayyum, Farhat Abbas, Fakhir Hannan, Jörg Rinklebe, Yong Sik Ok
Cadmium (Cd) is a well-known and widespread toxic heavy metal while the effects of biochar (BC) on Cd bioavailability and toxicity in wheat, especially in soils with aged contamination are largely unknown. In the present study, the effect of rice straw BC on Cd immobilization in soil and uptake by wheat in an agricultural contaminated-soil was investigated. Different levels of rice straw BC (0%, 1.5%, 3.0% and 5% w/w) were incorporated into the soil and incubated for two weeks. After this, wheat plants were grown in the amended soil until maturity...
February 20, 2017: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/28225095/high-index-hk0-faceted-platinum-concave-nanocubes-with-enhanced-peroxidase-like-activity-for-an-ultrasensitive-colorimetric-immunoassay-of-the-human-prostate-specific-antigen
#2
Zhuangqiang Gao, Shuzhen Lv, Mingdi Xu, Dianping Tang
Developing simple, high-efficiency non-enzyme bioassays is of great importance for modern analytical systems, but remains a significant challenge. One promising route is to utilize highly efficient nanocatalysts with the exposure of active crystal facets. Herein, we for the first time propose a novel colorimetric immunoassay for the ultrasensitive detection of the human prostate-specific antigen (PSA) based on using a unique type of nanolabel - high-index {hk0} faceted platinum concave nanocubes (HIF-Pt-CNCs)...
February 22, 2017: Analyst
https://www.readbyqxmd.com/read/28224016/characterization-of-major-metabolites-of-polymethoxylated-flavonoids-in-pericarpium-citri-reticulatae-using-liver-microsomes-immobilized-on-magnetic-nanoparticles-coupled-with-uplc-ms-ms
#3
Jun Lei, Ying Xue, Yi-Ming Liu, Xun Liao
The peels of citrus fruits (Pericarpium Citri Reticulatae, PCR) have long been used in traditional Chinese medicines (TCMs). Polymethoxylated flavonoids (PMFs) were found to be the main components present in PCR extracts, but their metabolism remains unclear which restrain the utilization of this TCM. In the present work, rat liver microsomes were immobilized on magnetic nanoparticles (LMMNPs) for in vitro metabolic study on the whole PMFs of PCR. LMMNPs were characterized by transmission electron microscope and Fourier-transform infrared spectrum...
2017: Chemistry Central Journal
https://www.readbyqxmd.com/read/28223134/investigation-of-activity-and-stability-of-papain-by-adsorption-on-multi-wall-carbon-nanotubes
#4
Ahmad Homaei, Fayezeh Samari
Papain was non- covalently immobilized on multi-walled carbon nanotubes (MWCNTs). Its stabilities and catalytic activity for casein degradation were comprehensively investigated. Compared to free papain, the nano- enzyme exhibited significantly improved thermal, pH and recycling stability. Comparisons of the kinetic parameters between free papain and the heterogeneous enzyme revealed that the Km value of the immobilized papain experienced a slight increase, which suggested that the MWCNTs did not significantly hinder papain's access to substrate or release of product...
February 13, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28220537/immobilization-of-horseradish-peroxidase-on-zno-nanowires-macroporous-sio2-composites-for-the-complete-decolorization-of-anthraquinone-dyes
#5
Huaiyan Sun, Xinyu Jin, Feng Jiang, Ruifeng Zhang
A Zinc Oxide (ZnO) nanowires/ macroporous Silicon dioxide (SiO2 ) composite was used as support to immobilize horseradish peroxidase (HRP) simply by in-situ cross-linking method. As cross-linker was adsorbed on the surface of ZnO nanowires, the cross-linked HRP was quite different from the traditional cross-linking enzyme aggregates (CLEAs) on both structure and catalytic performance. Among three epoxy compounds diethylene glycol diglycidyl ether (DDE) was the best cross-linker, with which the loading amount of HRP with pI of 5...
February 21, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28219688/mesoporous-phenylalanine-ammonia-lyase-microspheres-with-improved-stability-through-calcium-carbonate-templating
#6
Jiandong Cui, Yamin Zhao, Zhilei Tan, Cheng Zhong, Peipei Han, Shiru Jia
Cross-linked enzyme aggregates (CLEAs) have recently emerged as a promising method for enzyme immobilization due to its simplicity and low cost. However, a lack of good size and morphological control over the as-prepared CLEAs has limited their practical applications in some cases. Here, monodisperse spherical CLEAs of phenylalanine ammonia lyase (PAL microspheres) were prepared based on CaCO3 microtemplates. The preparation procedure involves filling porous CaCO3 microtemplates with the protein by salt precipitation, glutaraldehyde crosslinking, and dissolution of the microtemplates...
February 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28219472/immunodiagnosis-of-paramphistomosis-using-monoclonal-antibody-based-sandwich-elisa-for-detection-of-paramphistomum-gracile-circulating-16-kda-antigen
#7
Panat Anuracpreeda, Kullanid Tepsupornkul, Runglawan Chawengkirttikul
In this study, we have produced a monoclonal antibody (MoAb) against 16 kDa antigen of Paramphistomum gracile (16 kDaAgPg), and developed an accurate sandwich enzyme-linked immunosorbent assay (sandwich ELISA) for the detection of circulating 16 kDaAg in the serum and fecal samples from cattle naturally infected with P. gracile. MoAb 1D10 was immobilized on a microtitre plate, and the antigen in the samples was captured and detected with biotinylated rabbit anti-16 kDaAgPg antibody. The lower detection limit of sandwich ELISA was 3·5 pg mL-1, and no cross-reaction with other parasite antigens was evaluated...
February 21, 2017: Parasitology
https://www.readbyqxmd.com/read/28219031/aspartate-aminotransferase-is-potently-inhibited-by-copper-complexes-exploring-copper-complex-binding-proteome
#8
Yuqi Jia, Liping Lu, Caixia Yuan, Sisi Feng, Miaoli Zhu
Recent researches indicated that a copper complex-binding proteome that potently interacted with copper complexes and then influenced cellular metabolism might exist in organism. In order to explore the copper complex-binding proteome, a copper chelating ion-immobilized affinity chromatography (Cu-IMAC) column and mass spectrometry were used to separate and identify putative Cu-binding proteins in primary rat hepatocytes. A total of 97 putative Cu-binding proteins were isolated and identified. Five higher abundance proteins, aspartate aminotransferase (AST), malate dehydrogenase (MDH), catalase (CAT), calreticulin (CRT) and albumin (Alb) were further purified using a SP-, and (or) Q-Sepharose Fast Flow column...
February 11, 2017: Journal of Inorganic Biochemistry
https://www.readbyqxmd.com/read/28218499/fine-tuning-sortase-mediated-immobilization-of-protein-layers-on-surfaces-using-sequential-deprotection-and-coupling
#9
Maryam Raeeszadeh-Sarmazdeh, Ranganath Parthasarathy, Eric T Boder
Increasing interest in protein immobilization on surfaces has heightened the need for techniques enabling layer-by-layer protein attachment. Here, we report a technique for controlling enzyme-mediated immobilization of layers of protein on the surface using a genetically-encoded protecting group. An enterokinase-cleavable peptide sequence was inserted at the N-terminus of bifunctional fluorescent proteins containing Sortase A substrate recognition tags at both ends to control Sortase A-mediated protein immobilization on the surface layer-by-layer...
February 20, 2017: Biotechnology Progress
https://www.readbyqxmd.com/read/28215872/detection-of-specific-antibodies-in-cats-infected-with-the-lung-nematode-aelurostrongylus-abstrusus
#10
Eva-Maria Zottler, Christina Strube, Manuela Schnyder
Feline aelurostrongylosis, caused by the metastrongylid nematode Aelurostrongylus abstrusus, is an underestimated respiratory parasitosis. Its diagnosis currently mainly relies on the isolation of first stage larvae from fresh faecal samples. The aim of our study was to develop a serological test for the detection of specific antibodies against A. abstrusus by ELISA. We used recombinant major sperm protein (MSP) of the bovine lungworm Dictyocaulus viviparus as detection antigen and evaluated two different ELISA plates (Maxisorp and Immobilizer™ Amino-plate, Nunc Roskilde, Denmark) with two different enzyme systems [alkaline phosphatase (AP) and horseradish peroxidase (HRP)]...
February 15, 2017: Veterinary Parasitology
https://www.readbyqxmd.com/read/28215668/potential-of-surfactant-coated-nanoparticles-to-improve-brain-delivery-of-arylsulfatase-a
#11
Tilman Schuster, Astrid Mühlstein, Claudia Yaghootfam, Olga Maksimenko, Elena Shipulo, Svetlana Gelperina, Jörg Kreuter, Volkmar Gieselmann, Ulrich Matzner
The lysosomal storage disorder (LSD) metachromatic leukodystrophy (MLD) is caused by a deficiency of the soluble, lysosomal hydrolase arylsulfatase A (ASA). The disease is characterized by accumulation of 3-O-sulfogalactosylceramide (sulfatide), progressive demyelination of the nervous system and premature death. Enzyme replacement therapy (ERT), based on regular intravenous injections of recombinant functional enzyme, is in clinical use for several LSDs. For MLD and other LSDs with central nervous system (CNS) involvement, however, ERT is limited by the blood-brain barrier (BBB) restricting transport of therapeutic enzymes from the blood to the brain...
February 16, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28215563/chitosan-immobilized-novel-peroxidase-from-azadirachta-indica-characterization-and-application
#12
Veda P Pandey, Jyoti Rani, Nivedita Jaiswal, Swati Singh, Manika Awasthi, Ajit K Shasany, Sameeksha Tiwari, Upendra N Dwivedi
In the present paper, a peroxidase was purified from the leaves of a medicinal tree, namely Azadirachta indica, to 45.2 folds with overall recovery of 61%. Based on the subunit size, the purified peroxidase was suggested to be a monomeric structure of size 50kDa and exhibited good thermostability as it was fully stable at 65°C for 1hr and also retained about 73% activity at 70°C till 30min. The substrate affinity was found to be in order of guaiacol > pyrogallol > o-dianisidine. The purified peroxidase was found to be insensitive towards high concentrations of Na(+), Ca(2+), Mg(2+) and Mn(2+)...
February 16, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28215403/performance-comparison-of-three-trypsin-columns-used-in-liquid-chromatography
#13
Tereza Šlechtová, Martin Gilar, Květa Kalíková, Stephanie M Moore, James W Jorgenson, Eva Tesařová
Trypsin is the most widely used enzyme in proteomic research due to its high specificity. Although the in-solution digestion is predominantly used, it has several drawbacks, such as long digestion times, autolysis, and intolerance to high temperatures or organic solvents. To overcome these shortcomings trypsin was covalently immobilized on solid support and tested for its proteolytic activity. Trypsin was immobilized on bridge-ethyl hybrid silica sorbent with 300Å pores, packed in 2.1×30mm column and compared with Perfinity and Poroszyme trypsin columns...
February 14, 2017: Journal of Chromatography. A
https://www.readbyqxmd.com/read/28215070/influence-of-hierarchical-interfacial-assembly-on-lipase-stability-and-performance-in-deep-eutectic-solvent
#14
Stephanie M Andler, Li-Sheng Wang, Vincent M Rotello, Julie M Goddard
Hierarchical systems that integrate nano- and macroscale structural elements can offer enhanced enzyme stability over traditional immobilization methods. Microparticles were synthesized using interfacial assembly of lipase B from Candida antarctica with (CLMP-N) and without (CLMP) nanoparticles around a crosslinked polymeric core, to characterize the influence of the hierarchical assembly on lipase stability in extreme environments. Kinetic analysis revealed the turnover rate (kcat) significantly increased after immobilization...
February 20, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28214445/immobilization-of-aspergillus-oryzae-%C3%AE-galactosidase-in-an-agarose-matrix-functionalized-by-four-different-methods-and-application-to-the-synthesis-of-lactulose
#15
Cecilia Guerrero, Carlos Vera, Nestor Serna, Andrés Illanes
Aspergillus oryzae β-galactosidase was immobilized in monofunctional glyoxyl-agarose and heterofunctional supports (amino-glyoxyl, carboxy-glyoxyl and chelate-glyoxyl agarose), for obtaining highly active and stable catalysts for lactulose synthesis. Specific activities of the amino-glyoxyl agarose, carboxy-glyoxyl agarose and chelate-glyoxyl agarose derivatives were 3676, 430 and 454IU/g biocatalyst with half-life values at 50°C of 247, 100 and 100h respectively. Specific activities of 3490, 2559 and 1060IU/g were obtained for fine, standard and macro agarose respectively...
February 7, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28212269/separation-and-characterization-of-angiotensin-i-converting-enzyme-ace-inhibitory-peptides-from-saurida-elongata-proteins-hydrolysate-by-imac-ni-2
#16
Lixia Sun, Shanguang Wu, Liqin Zhou, Feng Wang, Xiongdiao Lan, Jianhua Sun, Zhangfa Tong, Dankui Liao
Lizard fish protein hydrolysates (LFPH) were prepared from Lizard fish (Saurida elongata) proteins possessing powerful angiotensin I converting enzyme (ACE) inhibitory activity and the fraction (LFPH-I) with high ACE inhibitory activity was obtained through ultrafiltration. The active Fraction (F2) was isolated from LFPH-I using immobilized metal affinity chromatography (IMAC-Ni(2+)). Analysis of amino acid levels revealed that F2 eluted from IMAC was enriched in Met, His, Tyr, Pro, Ile, and Leu compared to the crude peptide LFPH-I...
February 15, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28208715/biotransformation-and-detoxification-of-xylidine-orange-dye-using-immobilized-cells-of-marine-derived-lysinibacillus-sphaericus-d3
#17
Prabha Devi, Solimabi Wahidullah, Farhan Sheikh, Rochelle Pereira, Niteen Narkhede, Divya Amonkar, Supriya Tilvi, Ram Murthy Meena
Lysinibacillus sphaericus D3 cell-immobilized beads in natural gel sodium alginate decolorized the xylidine orange dye 1-(dimethylphenylazo)-2-naphthol-6-sulfonic acid sodium salt in the laboratory. Optimal conditions were selected for decolorization and the products formed were evaluated for toxicity by disc diffusion assay against common marine bacteria which revealed the non-toxic nature of the dye-degraded products. Decolorization of the brightly colored dye to colorless products was measured on an Ultra Violet-Vis spectrophotometer and its biodegradation products monitored on Thin Layer Chromatographic plate and High Performance Liquid Chromatography (HPLC)...
February 8, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28208644/novel-magnetic-cross-linked-cellulase-aggregates-with-a-potential-application-in-lignocellulosic-biomass-bioconversion
#18
Junqi Jia, Weiwei Zhang, Zengjie Yang, Xianling Yang, Na Wang, Xiaoqi Yu
The utilization of renewable biomass resources to produce high-value chemicals by enzymatic processes is beneficial for alternative energy production, due to the accelerating depletion of fossil fuels. As immobilization techniques can improve enzyme stability and reusability, a novel magnetic cross-linked cellulase aggregate has been developed and applied for biomass bioconversion. The crosslinked aggregates could purify and immobilize enzymes in a single operation, and could then be combined with magnetic nanoparticles (MNPs), which provides easy separation of the materials...
February 10, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28208260/simultaneous-enhancement-of-bioactivity-and-stability-of-laccase-by-cu-2-paa-ppega-matrix-for-efficient-biosensing-and-recyclable-decontamination-of-pyrocatechol
#19
Tao Chen, Yuanhong Xu, Zhi Peng, Aihua Li, Jingquan Liu
Simultaneously enhancing the catalytic bioactivity and stability of enzyme is still an intractable issue in the enzymatic study. Herein, a facile and effective approach was designed to immobilize and modify laccase on a Cu(2+)-adsorbed pyrene-terminated block copolymer [poly(acrylic acid)/poly(poly(ethylene glycol) acrylate)] (PAA/PPEGA), which was prepared via well-controlled reversible addition-fragmentation chain transfer polymerization. PAA provided the supporting matrix for firm immobilization of Cu(2+), an enzyme bioactivity inducer, onto the microstructure of laccase, while avoiding any contamination of the heavy metal Cu(2+) into the following application system...
February 7, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28198433/a-cyclometalated-iridium-iii-complex-used-as-a-conductor-for-the-electrochemical-sensing-of-ifn-%C3%AE
#20
Xiangmin Miao, Chung-Nga Ko, Kasipandi Vellaisamy, Zongbing Li, Guanjun Yang, Chung-Hang Leung, Dik-Lung Ma
A novel iridium(III) complex was prepared and used as a conductor for sensitive and enzyme-free electrochemical detection of interferon gamma (IFN-γ). This assay is based on a dual signal amplification mechanism involving positively charged gold nanoparticles ((+)AuNPs) and hybridization chain reaction (HCR). To construct the sensor, nafion (Nf) and (+)AuNPs composite membrane was first immobilized onto the electrode surface. Subsequently, a loop-stem structured capture probe (CP) containing a special IFN-γ interact strand was modified onto the (+)AuNP surface via the formation of Au-S bonds...
February 15, 2017: Scientific Reports
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