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Enzyme immobilization

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https://www.readbyqxmd.com/read/29784306/enzymatic-hydrolysis-of-flaxseed-linum-usitatissimum-l-protein-and-sensory-characterization-of-maillard-reaction-products
#1
Chao-Kun Wei, Kiran Thakur, Dun-Hua Liu, Jian-Guo Zhang, Zhao-Jun Wei
We aimed to simplify the enzymolysis process for flaxseed protein hydrolysates production as Maillard reaction products (MRPs) to generate different flavor characteristics. More than 50% activity of immobilized enzymes (Alcalase and Flavourzyme) was retained after repeated use. Subsequently, after five weeks, the activities of the immobilized enzymes were also observed to be higher after storage at 4 °C. The optimum conditions for production of flaxseed protein hydrolysates using sequential enzymatic hydrolysis were as follow: 3,000 U/g of Alcalase at 60 °C and pH 8...
October 15, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29783080/development-and-characterization-of-a-field-effect-biosensor-for-the-detection-of-acetoin
#2
Denise Molinnus, Lukas Muschallik, Laura Osorio Gonzalez, Johannes Bongaerts, Torsten Wagner, Thorsten Selmer, Petra Siegert, Michael Keusgen, Michael J Schöning
A capacitive electrolyte-insulator-semiconductor (EIS) field-effect biosensor for acetoin detection has been presented for the first time. The EIS sensor consists of a layer structure of Al/p-Si/SiO2 /Ta2 O5 /enzyme acetoin reductase. The enzyme, also referred to as butane-2,3-diol dehydrogenase from B. clausii DSM 8716T , has been recently characterized. The enzyme catalyzes the (R)-specific reduction of racemic acetoin to (R,R)- and meso-butane-2,3-diol, respectively. Two different enzyme immobilization strategies (cross-linking by using glutaraldehyde and adsorption) have been studied...
May 18, 2018: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29783053/biomineralization-mechanism-of-u-vi-induced-by-bacillus-cereus-12-2-the-role-of-functional-groups-and-enzymes
#3
Jian Zhang, Han Song, Zhi Chen, Shasha Liu, Yali Wei, Jingyi Huang, Chuling Guo, Zhi Dang, Zhang Lin
Current studies reveal that the biomineralization of U(VI) by anaerobes normally produces nano-sized U(IV) minerals that can easily re-migrate/re-oxidize, while the biomineralization of U(VI) by aerobes has been constrained because the general mechanism has not yet been fully characterized. The biomineralization of U(VI) by Bacillus cereus 12-2 was investigated in this work. The maximum biosorption capability of intact cells was 448.68 mg U/g biomass (dry weight) at pH 5, while a decrease over 60% was induced when phosphate, amino, and especially carboxyl groups were shielded...
May 10, 2018: Chemosphere
https://www.readbyqxmd.com/read/29781041/a-bead-based-immunogold-silver-staining-assay-on-capillary-driven-microfluidics
#4
Ngoc M Pham, Sebastian Rusch, Yuksel Temiz, Robert D Lovchik, Hans-Peter Beck, Walter Karlen, Emmanuel Delamarche
Point-of-care (POC) diagnostics are critically needed for the detection of infectious diseases, particularly in remote settings where accurate and appropriate diagnosis can save lives. However, it is difficult to implement immunoassays, and specifically immunoassays relying on signal amplification using silver staining, into POC diagnostic devices. Effective immobilization of antibodies in such devices is another challenge. Here, we present strategies for immobilizing capture antibodies (cAbs) in capillary-driven microfluidic chips and implementing a gold-catalyzed silver staining reaction...
May 21, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/29779107/stabilization-of-butyrylcholinesterase-by-the-entrapment-into-the-natural-polymer-based-gels
#5
V I Lonshakova-Mukina, E N Esimbekova, V A Kratasyuk
A new method for obtaining stable butyrylcholinesterase (BuChE) samples based on the enzyme immobilization in starch and gelatin gels followed by drying is proposed. Coimmobilization of BuChE with the thiol group indicator 5,5'-dithiobis(2-nitrobenzoic) acid did not reduce the activity of BuChE, which allowed us to simplify the procedure and reduce the time of analysis of organophosphorus pesticides. The resulting immobilized samples retained activity for at least 300 days. BuChE samples based on the starch gel showed a greater sensitivity in the determination of pesticides as compared to the samples based on the gelatin gel...
March 2018: Doklady. Biochemistry and Biophysics
https://www.readbyqxmd.com/read/29778024/simultaneous-glucose-production-from-cellulose-and-fouling-reduction-using-a-magnetic-responsive-membrane-reactor-with-superparamagnetic-nanoparticles-carrying-cellulolytic-enzymes
#6
Abaynesh Yihdego Gebreyohannes, Madhav Dharmjeet, Tom Swusten, Matthias Mertens, Joran Verspreet, Thierry Verbiest, Christophe M Courtin, Ivo F J Vankelecom
This work aimed at investigating simultaneous hydrolysis of cellulose and in-situ foulant degradation in a cellulose fed superparamagnetic biocatalytic membrane reactor (BMRSP ). In this reactor, a dynamic layer of superparamagnetic bionanocomposites with immobilized cellulolytic enzymes were reversibly immobilized on superparamagnetic polymeric membrane using an external magnetic field. The formation of a dynamic layer of bionanocomposites on the membrane helped to prevent direct membrane-foulant interaction...
May 2, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29777816/aptamer-based-assay-for-monitoring-genetic-disorder-phenylketonuria-pku
#7
Mohammad Hasanzadeh, Amir Zargami, Hossein Navay Baghban, Ahad Mokhtarzadeh, Nasrin Shadjou, Soltanali Mahboob
The genetic disorder phenylketonuria (PKU) is the inability to metabolize phenylalanine because of a lack of the enzyme phenylalanine hydroxylase. Phenylalanine is used to biochemically form proteins, coded for by DNA. The development of an apta-assay for detection of l-Phenylalanine is presented in this work. A highly specific DNA-aptamer, selected to l-Phenylalanine was immobilized onto a gold nanostructure and electrochemical measurements were performed in a solution containing the phosphate buffer solution with physiological pH...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29777804/towards-an-affordable-enzymatic-production-of-biopolyols-comparing-the-immobilization-of-lipases-by-two-optimized-techniques
#8
Milda Šulcienė, Boris Kolvenbach, Erik Ammann, Inga Matijošytė
The production of biopolyols (sustainable, renewable and biodegradable precursors), is exclusively focused on the conventional chemical synthesis pathways, and currently, no polyols are produced by enzymatic processes at an industrial scale, because they are not competitive when compared to petrochemically based polyols due to the overall cost for their production. This can be mainly attributed to the high costs of commercial enzymes used in this process and their use is limited by their lack of stability during the bioprocess...
May 16, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29771598/understanding-the-potential-benefits-of-thyme-and-their-derived-products-on-food-industry-and-health-from-extraction-of-high-added-value-compounds-to-the-evaluation-of-bioaccessibility-bioavailability-anti-inflammatory-and-antimicrobial-activities
#9
Jose M Lorenzo, Amin Mousavi Khaneghah, Mohsen Gavahian, Krystian Marszałek, Ismail Eş, Paulo E S Munekata, Isabel C F R Ferreira, Francisco J Barba
Natural bioactive compounds isolated from aromatic plants have been studied for centuries due to their unique characteristics that carry great importance in food, pharmaceutical, and cosmetic industry. For instance, several beneficial activities have been attributed to some specific compounds found in Thymus such as anti-inflammatory, antioxidant, antimicrobial and antiseptic properties. Moreover, these compounds are classified as Generally Recognized as Safe (GRAS). Conventional extraction processes of these compounds and their derived forms from thyme leaves are well established, however, they present some important drawbacks such as long extraction time, low yield, high solvent consumption and unfavorable effect on thermolabile compounds...
May 17, 2018: Critical Reviews in Food Science and Nutrition
https://www.readbyqxmd.com/read/29769829/creatine-supplementation-supports-the-rehabilitation-of-adolescent-fin-swimmers-in-tendon-overuse-injury-cases
#10
Imre Juhasz, Judit Plachy Kopkane, Pal Hajdu, Gabor Szalay, Bence Kopper, Jozsef Tihanyi
Our purpose was to investigate the effect of creatine (Cr) supplementation on regeneration periods in tendon overuse injury rehabilitation of adolescent fin swimmers. The participants of this study were injured adolescent competitive fin swimmers (n = 18). The subjects were randomly assigned the creatine (CR) or placebo (PL) groups with a double-blind research design. The subjects were given Cr supplementation or received the placebo as part of the conservative treatment of the tendinopathy. We measured the segmental lean mass (SLM;kg), the ankle plantar flexion peak torque (PFT;N·m), the pain intensity (NRS;values), prior to immobilization, after immobilization (R2) and after the 2nd (R4) and 4th (R6) weeks of the rehabilitation period of the injured limb...
June 2018: Journal of Sports Science & Medicine
https://www.readbyqxmd.com/read/29768874/nanocarriers-immobilized-proteases-and-their-industrial-applications-an-overview
#11
Qayyum Husain
The enzymes proteases have attracted a lot of attention due to their high demand in various industrial and other sectors since past several decades. Nanosupport bound proteases showed high efficiency of binding and yield of immobilization. Enzymes attached to such support were found significantly more resistant to the denaturation mediated by change in pH and temperature, medium particularly in the presence of water miscible and immiscible organic solvents, detergents, and several kinds of other denaturants...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29768820/enzyme-immobilization-in-mesoporous-silica-for-enhancement-of-thermostability
#12
Shun-Ichi Matsuura, Manami Chiba, Tatsuo Tsunoda, Aritomo Yamaguchi
Direct enzyme immobilization by encapsulation in the pores of mesoporous silica particles enhances protein thermal and chemical stability. In this study, we investigated the effect of pore size on the thermostability and catalytic activity of Escherichia coli glutaminase YbaS encapsulated under high temperature conditions in two SBA-type mesoporous silicas: SBA5.4 and SBA10.6 with pore diameters of 5.4 and 10.6 nm, respectively. The changes in enzyme conformation under high temperature conditions were assessed using PSA, a benzophenoxazine-based fluorescent dye that is sensitive to denatured aggregated proteins...
January 1, 2018: Journal of Nanoscience and Nanotechnology
https://www.readbyqxmd.com/read/29768002/synergistic-enhancement-of-enzyme-performance-and-resilience-via-orthogonal-peptide-protein-chemistry-enabled-multilayer-construction
#13
Xue-Jian Zhang, Xiao-Wei Wang, Jiaxing Sun, Chao Su, Shuguang Yang, Wen-Bin Zhang
Protein immobilization is critical to utilize their unique functions in diverse applications. Herein, we report that orthogonal peptide-protein chemistry enabled multilayer construction can facilitate the incorporation of various folded structural domains, including calmodulin in different states, affibody and dihydrofolate reductase (DHFR). An extended conformation is found to be the most advantageous for steady film growth. The resulting protein thin films exhibit sensitive and selective responsive behaviors to bio-signals (Ca2+, TFP, NADPH, etc...
May 16, 2018: Biomacromolecules
https://www.readbyqxmd.com/read/29767959/stabilization-of-immobilized-enzymes-via-the-chaperone-like-activity-of-mixed-lipid-bilayers
#14
Andres F Chaparro Sosa, Daniel F Kienle, Rebecca M Falatach, Jessica Flanagan, Joel L Kaar, Daniel K Schwartz
Biomimetic lipid bilayers represent intriguing materials for enzyme immobilization, which is critical for many biotechnological applications. Here, through the creation of mixed lipid bilayers, the retention of immobilized enzyme structure and catalytic activity are dramatically enhanced. The enhancement in the retention of enzyme structure, which correlated with an increase in enzyme activity, is observed using dynamic single-molecule (SM) fluorescence methods. The results of SM analysis specifically show that lipid bilayers composed of mixtures of 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (DOPG) stabilize the folded state of nitroreductase (NfsB) increasing the rate of re-folding relative to unfolding of enzyme molecules on the bilayer surface...
May 16, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29766828/protective-effects-of-crocetin-on-depression-like-behavior-induced-by-immobilization-in-rat
#15
Tahereh Farkhondeh, Saeed Samarghandian, Fariborz Samini, Ali Rajabpour Sanati
Crocetin, an active ingredient of saffron, has been recognized as a potent antioxidant. Plant extracts or their components may be useful in ameliorating the various diseases, including neurodegenerative disorders. This study investigated the effects of crocetin on oxidative damage induced by chronic restraint stress in the rat brain. For this reason, rats were kept in the restrainers for 1 hour every day, for 21 consecutive days. The animals were injected of crocetin (20, 40, 60 mg/kg) or vehicle daily for 21 days...
May 15, 2018: CNS & Neurological Disorders Drug Targets
https://www.readbyqxmd.com/read/29766370/continuous-laminaribiose-production-using-an-immobilized-bienzymatic-system-in-a-packed-bed-reactor
#16
Akram Abi, Anqi Wang, Hans-Joachim Jördening
The first continuous production system of laminaribiose from sucrose and glucose in a bienzymatic reaction is reported in this study. Immobilized laminaribiose phosphorylase and sucrose phosphorylase were used in a packed bed reactor system comprising of a 3-cm glass column at 35 °C with a steady feeding flow rate of 0.1 ml/min. Factors affecting product formation including enzyme ratio, peal concept (both enzymes in one pearl or in separate pearls), and pearl size were studied. An enzyme ratio of 2:1 of laminaribiose phosphorylase (LP) to sucrose phosphorylase (SP) when encapsulated separately in bigger size peals resulted in higher concentration of product...
May 16, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/29759237/evaluation-of-the-activity-of-%C3%AE-glucosidase-immobilized-on-polydimethylsiloxane-pdms-with-a-microfluidic-flow-injection-analyzer-with-embedded-optical-fibers
#17
Francisco M Hernández-Maya, María P Cañizares-Macías
β-glucosidase from almonds was immobilized on a polydimethylsiloxane (PDMS) microdevice by covalent chain using 3-aminopropyltrietoxysilane and glutaraldehyde. Enzymatic activity was evaluated using p-nitro-phenyl-β-D-glucopyranoside dissolved in a 0.01 M pH 5.0 phosphate solution at 45 °C measuring the reaction product (p-nitrophenol) at 410 nm. The microdevice consisted of two parts: the one part where the enzymatic reaction was carried out and a second part where pH was adjusted at 10, with NaOH...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29759183/evaluation-of-thrombin-inhibitory-activity-of-catechins-by-online-capillary-electrophoresis-based-immobilized-enzyme-microreactor-and-molecular-docking
#18
Qiao-Qiao Li, Feng-Qing Yang, Yin-Zhen Wang, Zhao-Yu Wu, Zhi-Ning Xia, Hua Chen
An online capillary electrophoresis (CE)-based thrombin (THR) immobilized enzyme microreactor (IMER) method was established to screen THR inhibitors in this study. S-2366 was used as chromogenic substrate for determination of THR activity and other kinetic constants. After continuously run for 50 times, the prepared IMER could still remain 89% of the initial immobilized enzyme activity. The Michaelis-Menten constant (Km ) of immobilized THR was measured as 0.514 mmol/L and the half-maximal inhibitory concentration (IC50 ) and inhibition constant (Ki ) of argatroban on THR were determined as 78...
August 1, 2018: Talanta
https://www.readbyqxmd.com/read/29758537/biopolymers-conjugated-with-magnetite-as-support-materials-for-trypsin-immobilization-and-protein-digestion
#19
Jakub Zdarta, Katarzyna Antecka, Artur Jędrzak, Karol Synoradzki, Magdalena Łuczak, Teofil Jesionowski
In the presented study synthesized magnetic nanoparticles were used as an inorganic precursor for the preparation of novel magnetite-lignin and magnetite-chitin hybrid supports for enzyme immobilization. Effective synthesis of the hybrids was confirmed by Fourier transform infrared spectroscopy and powder X-ray diffraction analysis. The materials exhibited good thermal stability and surface areas of 4.3 and 5.6 m2 /g respectively. The magnetite-lignin + trypsin and magnetite-chitin + trypsin systems were found to have good storage stability and reusability...
May 8, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29758351/development-of-microreactors-with-surface-immobilized-biocatalysts-for-continuous-transamination
#20
Nataša Miložič, Gorazd Stojkovič, Andreas Vogel, Dominique Bouwes, Polona Žnidaršič-Plazl
The industrial importance of optically pure compounds has thrown a spotlight on ω-transaminases that have shown a high potential for the synthesis of bioactive compounds with a chiral amine moiety. The implementation of biocatalysts in industrial processes relies strongly on fast and cost effective process development, including selection of a biocatalyst form and the strategy for its immobilization. Here, microscale reactors with selected surface-immobilized amine-transaminase (ATA) in various forms are described as platforms for high-throughput process development...
May 11, 2018: New Biotechnology
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