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Enzyme immobilization

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https://www.readbyqxmd.com/read/28918231/highly-sensitive-surface-plasmon-resonance-biosensor-for-the-detection-of-hiv-related-dna-based-on-dynamic-and-structural-dna-nanodevices
#1
Wei Diao, Min Tang, Shijia Ding, Xinmin Li, Wenbin Cheng, Fei Mo, Xiaoyu Yan, Hongmin Ma, Yurong Yan
Early detection, diagnosis and treatment of human immune deficiency virus (HIV) infection is the key to reduce acquired immunodeficiency syndrome (AIDS) mortality. In our research, an innovative surface plasmon resonance (SPR) biosensing strategy has been developed for highly sensitive detection of HIV-related DNA based on entropy-driven strand displacement reactions (ESDRs) and double-layer DNA tetrahedrons (DDTs). ESDRs as enzyme-free and label-free signal amplification circuit can be specifically triggered by target DNA, leading to the cyclic utilization of target DNA and the formation of plentiful double-stranded DNA (dsDNA) products...
September 9, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28917970/how-do-enzymes-meet-nanoparticles-and-nanomaterials
#2
REVIEW
Ming Chen, Guangming Zeng, Piao Xu, Cui Lai, Lin Tang
Enzymes are fundamental biological catalysts responsible for biological regulation and metabolism. The opportunity for enzymes to 'meet' nanoparticles and nanomaterials is rapidly increasing due to growing demands for applications in nanomaterial design, environmental monitoring, biochemical engineering, and biomedicine. Therefore, understanding the nature of nanomaterial-enzyme interactions is becoming important. Since 2014, enzymes have been used to modify, degrade, or make nanoparticles/nanomaterials, while numerous nanoparticles/nanomaterials have been used as materials for enzymatic immobilization and biosensors and as enzyme mimicry...
September 13, 2017: Trends in Biochemical Sciences
https://www.readbyqxmd.com/read/28917965/the-development-of-a-butyrylcholinesterase-porous-pellet-for-innovative-detection-of-cholinesterase-inhibitors
#3
Jiří Zeman, David Vetchý, Aleš Franc, Sylvie Pavloková, Vladimír Pitschmann, Lukáš Matějovský
The aim of the presented research was the preparation of an innovative carrier with significantly improved properties for the fast and sensitive detection of cholinesterase inhibitors such as nerve agents. This innovative carrier was in the form of spherical pellets containing different amounts of Neusilin. Neusilin is a synthetic and amorphous form of magnesium aluminometasilicate with a high specific surface area, and the immobilized enzyme butyrylcholinesterase with an activity of 50nkat·g(-1). Pellets were prepared by the extrusion-spheronization method and dried in a hot air oven under two conditions - at 30°C for 72h and at 60°C for 24h...
September 13, 2017: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28917804/development-and-validation-of-a-maleimide-based-enzyme-linked-immunosorbent-assay-for-the-detection-of-tetrodotoxin-in-oysters-and-mussels
#4
Laia Reverté, Maria Rambla-Alegre, Sandra Leonardo, Carlos Bellés, Katrina Campbell, Christopher T Elliott, Arjen Gerssen, Mirjam D Klijnstra, Jorge Diogène, Mònica Campàs
The recent detection of tetrodotoxins (TTXs) in puffer fish and shellfish in Europe highlights the necessity to monitor the levels of TTXs in seafood by rapid, specific, sensitive and reliable methods in order to protect human consumers. A previous immunoassay for TTX detection in puffer fish, based on the use of self-assembled monolayers (SAMs) for the immobilization of TTX on maleimide plates (mELISA), has been modified and adapted to the analysis of oyster and mussel samples. Changing dithiol for cysteamine-based SAMs enabled reductions in the assay time and cost, while maintaining the sensitivity of the assay...
January 1, 2018: Talanta
https://www.readbyqxmd.com/read/28917047/detection-of-selenoproteins-by-laser-ablation-inductively-coupled-plasma-mass-spectrometry-la-icp-ms-in-immobilized-ph-gradient-ipg-strips
#5
Jordan Sonet, Sandra Mounicou, Laurent Chavatte
In contrast to other trace elements that are cofactors of enzymes and removed from proteins under denaturing conditions, Se is covalently bound to proteins when incorporated into selenoproteins, since it is a component of selenocysteine aminoacid. It implies that selenoproteins can undergo several biochemical separation methods in stringent and chaotropic conditions and still maintain the presence of selenium in the primary sequence. This feature has been used to develop a method for the detection of trace levels of human selenoproteins in cell extracts without the use of radioactive isotopes...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28914766/development-of-a-sensitive-electrochemical-enzymatic-reaction-based-cholesterol-biosensor-using-nano-sized-carbon-interdigitated-electrodes-decorated-with-gold-nanoparticles
#6
Deepti Sharma, Jongmin Lee, Junyoung Seo, Heungjoo Shin
We developed a versatile and highly sensitive biosensor platform. The platform is based on electrochemical-enzymatic redox cycling induced by selective enzyme immobilization on nano-sized carbon interdigitated electrodes (IDEs) decorated with gold nanoparticles (AuNPs). Without resorting to sophisticated nanofabrication technologies, we used batch wafer-level carbon microelectromechanical systems (C-MEMS) processes to fabricate 3D carbon IDEs reproducibly, simply, and cost effectively. In addition, AuNPs were selectively electrodeposited on specific carbon nanoelectrodes; the high surface-to-volume ratio and fast electron transfer ability of AuNPs enhanced the electrochemical signal across these carbon IDEs...
September 15, 2017: Sensors
https://www.readbyqxmd.com/read/28911636/novel-electrochemical-xanthine-biosensor-based-on-chitosan-polypyrrole-gold-nanoparticles-hybrid-bio-nanocomposite-platform
#7
Muamer Dervisevic, Esma Dervisevic, Emre Çevik, Mehmet Şenel
The aim of this study was the electrochemical detection of the adenosine-3-phosphate degradation product, xanthine, using a new xanthine biosensor based on a hybrid bio-nanocomposite platform which has been successfully employed in the evaluation of meat freshness. In the design of the amperometric xanthine biosensor, chitosan-polypyrrole-gold nanoparticles fabricated by an in situ chemical synthesis method on a glassy carbon electrode surface was used to enhance electron transfer and to provide good enzyme affinity...
July 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911617/hydrolysis-of-isoflavone-in-black-soy-milk-using-cellulose-bead-as-enzyme-immobilizer
#8
Kuan-I Chen, Yijun Yao, Hong-Jhang Chen, Yi-Chen Lo, Roch-Chui Yu, Kuan-Chen Cheng
The establishment of a catalytic system to enrich isoflavone aglycones in black soybean milk was investigated in this study. Beta-glucosidase, which was covalently immobilized onto cellulose beads, exhibited a significant efficiency for the conversion of 4-nitrophenyl β-d-glucuronide to p-nitrophenol over the sol-gel method. The Michaelis constant (Km) of the cellulose bead enzymatic system was determined to be 1.50±0.10 mM. Operational reusability of the cellulose bead enzymatic system was justified for more than 10 batch reactions in black soy milk...
October 2016: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28911538/corrigendum-to-hydrolysis-of-isoflavone-in-black-soy-milk-using-cellulose-bead-as-enzyme-immobilizer-j-food-drug-anal-24-2016-788-795
#9
Kuan-I Chen, Yijun Yao, Hong-Jhang Chen, Yi-Chen Lo, Roch-Chui Yu, Kuan-Chen Cheng
No abstract text is available yet for this article.
January 2017: Journal of Food and Drug Analysis
https://www.readbyqxmd.com/read/28910866/an-aptamer-based-electrochemical-sensor-that-can-distinguish-influenza-virus-subtype-h1-from-h5
#10
Jin-Moo Lee, JunWon Kim, Ilhwan Ryu, Hye-Min Woo, Tae Gyun Lee, Woong Jung, Sanggyu Yim, Yong-Joo Jeong
The surface protein hemagglutinin (HA) mediates the attachment of influenza virus to host cells containing sialic acid and thus facilitate viral infection. Therefore, HA is considered as a good target for the development of diagnostic tools for influenza virus. Previously, we reported the isolation of single-stranded aptamers that can distinguish influenza subtype H1 from H5. In this study, we described a method for the selective electrical detection of H1 using the isolated aptamer as a molecular probe. After immobilization of the aptamer on Si wafer, enzyme-linked immunosorbent assay (ELISA) and field emission scanning electron microscope (FE-SEM) showed that the immobilized aptamer specifically bound to only the H1 subtype but not the H5 subtype...
September 15, 2017: Journal of Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28905328/lessons-learned-from-protein-aggregation-toward-technological-and-biomedical-applications
#11
REVIEW
César L Avila, Silvina Chaves, Sergio B Socias, Esteban Vera-Pingitore, Florencia González-Lizárraga, Cecilia Vera, Diego Ploper, Rosana Chehín
The close relationship between protein aggregation and neurodegenerative diseases has been the driving force behind the renewed interest in a field where biophysics, neurobiology and nanotechnology converge in the study of the aggregate state. On one hand, knowledge of the molecular principles that govern the processes of protein aggregation has a direct impact on the design of new drugs for high-incidence pathologies that currently can only be treated palliatively. On the other hand, exploiting the benefits of protein aggregation in the design of new nanomaterials could have a strong impact on biotechnology...
September 13, 2017: Biophysical Reviews
https://www.readbyqxmd.com/read/28903175/chelant-enhanced-washing-of-cca-contaminated-soil-coupled-with-selective-dissolution-or-soil-stabilization
#12
Jingzi Beiyuan, Abbe Y T Lau, Daniel C W Tsang, Weihua Zhang, Chih-Ming Kao, Kitae Baek, Yong Sik Ok, Xiang-Dong Li
Remediation of CCA-contaminated soil (Cr, Cu, and As) by biodegradable chelant-enhanced washing (EDDS, S,S-ethylene-diamine-disuccinic-acid) needs further enhancement. This study investigated the effectiveness of coupling with pre-treatment by selective dissolution and post-treatment by soil amendments, respectively. Three groups of reagents (reductants, alkaline solvents, and organic ligands) were adopted in the pre-treatment to dissolve the oxide minerals before EDDS extraction. In the post-treatment, soil amendments (coal fly ash (CFA), acid mine drainage sludge (AMDS), green waste compost (GWC)), and their mixtures) were used for a 2-month stabilization after 2-h EDDS washing...
September 9, 2017: Science of the Total Environment
https://www.readbyqxmd.com/read/28899486/immobilization-on-graphene-oxide-improves-the-thermal-stability-and-bioconversion-efficiency-of-d-psicose-3-epimerase-for-rare-sugar-production
#13
Samir R Dedania, Manisha J Patel, Dijit M Patel, Rekha C Akhani, Darshan H Patel
D-Psicose (D-ribo-2-hexulose or D-allulose), an epimer of D-fructose is considered as a rare low-calorie sugar displaying important physiological functions. Enzymatic production using ketose 3-epimerases is the feasible process for the production of D-Psicose. However, major drawbacks in application of ketose 3-epimerases are bioconversion efficiency and reusability of the enzyme. We have attempted immobilization of ketose 3-epimerases from Agrobacterium tumefaciens (agtu) D-psicose 3-epimerase (DPEase) on graphene oxide...
December 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28899483/preparation-activity-and-structure-of-cross-linked-enzyme-aggregates-cleas-with-nanoparticle
#14
Shuguang Wang, Debing Zheng, Lingyu Yin, Fei Wang
Cross-linked enzyme aggregates (CLEAs) have emerged as an interesting biocatalyst design for enzyme immobilization. However, the commercialization of CLEAs is often hampered by their shortcomings, such as poor-controlled particle size, low activity and sticky characteristic. In order to overcome these drawbacks, five nanoparticles (NPs) (nano-TiO2, nano-MgO, nano-Ni, nano-Cu and nano-Fe3O4) were used to improve CLEAs activity and structure in this study. Results showed that moderate dosage of nano-TiO2 addition increased CLEAs activity, and the most increment was 15...
December 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/28893251/identification-and-immobilization-of-a-novel-cold-adapted-esterase-and-its-potential-for-bioremediation-of-pyrethroid-contaminated-vegetables
#15
Xinjiong Fan, Weiqu Liang, Yanfang Li, He Li, Xiaolong Liu
BACKGROUND: Pyrethroids are potentially harmful to living organisms and ecosystems. Thus, concerns have been raised about pyrethroid residues and their persistence in agricultural products. To date, although several pyrethroid-hydrolyzing enzymes have been cloned, very few reports are available on pyrethroid-hydrolyzing enzymes with cold adaptation, high hydrolytic activity and good reusability, indispensable properties in practical bioremediation of pyrethroid-contaminated vegetables...
September 11, 2017: Microbial Cell Factories
https://www.readbyqxmd.com/read/28889752/covalent-immobilization-of-oxylipin-biosynthetic-enzymes-on-nanoporous-rice-husk-silica-for-production-of-cis-12-oxophytodienoic-acid
#16
Thu Bao Le, Chong Soo Han, Kyoungwon Cho, Oksoo Han
Soybean lipoxygenase, recombinant rice allene oxide synthase-1 and rice allene oxide cyclase were covalently immobilized on nanoporous rice husk silica using two types of linkers: glutardialdehyde and polyethylene glycol. The immobilization efficiency achieved using glutardialdehyde-linked rice husk silica was higher than that achieved using polyethylene glycol-linked rice husk silica (50-92% and 25-50%, respectively). Immobilization on both types of matrices significantly decreased the specific activities of the immobilized enzymes...
September 11, 2017: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/28888544/xylanase-immobilization-on-modified-superparamagnetic-graphene-oxide-nanocomposite-effect-of-pegylation-on-activity-and-stability
#17
Vajihe Mehnati-Najafabadi, Asghar Taheri-Kafrani, Abdol-Khalegh Bordbar
In order to utilize the advantages of immobilization such as improvement of stability, increasing the catalytic activity, ability to recovery and reuse of enzyme from reaction medium, xylanase enzyme was immobilized on superparamagnetic garphene oxide nanosheets (GOMNP). Xylanase, as a hydrolytic enzyme of xylan has widely used in industry. Since the xylan is bulk, for enhance accessibility of active sites of the immobilized xylanase, polyethylene glycol bis amine (PEGA) was used as a spacer for functionalization of GOMNP...
September 6, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28888230/effect-of-supercritical-carbon-dioxide-on-the-enzymatic-production-of-biodiesel-from-waste-animal-fat-using-immobilized-candida-antarctica-lipase-b-variant
#18
Aldricho Alpha Pollardo, Hong-Shik Lee, Dohoon Lee, Sangyong Kim, Jaehoon Kim
BACKGROUND: Waste animal fat is a promising feedstock to replace vegetable oil that widely used in commercial biodiesel process, however the high content of free fatty acid in waste fat makes it unfeasible to be processed with commercial base-catalytic process. Enzymatic process is preferable to convert waste fat into biodiesel since enzyme can catalyze both esterification of free fatty acid and transesterification of triglyceride. However, enzymatic reaction still has some drawbacks such as lower reaction rates than base-catalyzed transesterification and the limitation of reactant concentration due to the enzyme inhibition of methanol...
September 9, 2017: BMC Biotechnology
https://www.readbyqxmd.com/read/28888015/functionalization-of-titanium-dioxide-nanotubes-with-biomolecules-for-biomedical-applications
#19
REVIEW
Weslley F Oliveira, Isabel R S Arruda, Germana M M Silva, Giovanna Machado, Luana C B B Coelho, Maria T S Correia
Titanium (Ti) and its alloys are extensively used in the manufacture of implants because they have biocompatibility. The production of a nanostructured surface can be achieved by means of titanium dioxide nanotubes (TNTs) which can have dimensions equivalent to the nanometric components of human bone, in addition to increasing the efficiency of such implants. The search is ongoing for ways to improve the performance of these TNTs in terms of their functionalization through coating these nanotubular matrices with biomolecules...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28884170/sers-active-metal-dielectric-nanostructures-integrated-in-microfluidic-devices-for-label-free-quantitative-detection-of-mirna
#20
Chiara Novara, Alessandro Chiadò, Niccolò Paccotti, Silvia Catuogno, Carla Lucia Esposito, Gerolama Condorelli, Vittorio De Franciscis, Francesco Geobaldo, Paola Rivolo, Fabrizio Giorgis
In this work, SERS-based microfluidic PDMS chips integrating silver-coated porous silicon membranes were used for the detection and quantitation of microRNAs (miRNAs), which consist of short regulatory non-coding RNA sequences typically over- or under-expressed in connection with several diseases such as oncogenesis. In detail, metal-dielectric nanostructures which provide noticeable Raman enhancements were functionalized according to a biological protocol, adapted and optimized from an enzyme-linked immunosorbent assay (ELISA), for the detection of miR-222...
September 8, 2017: Faraday Discussions
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